Carg04428 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04428
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	2-methylene-furan-3-one reductase-like
Location	Carg_Chr04: 20447761 .. 20449657 (-)
RNA-Seq Expression	Carg04428
Synteny	Carg04428

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCCCATCCATGGCTGCTCCTTCTGTTCCCACCCACATGAAGGCTTGGGTCTACCATGATTTCGGCAACACTGCCGATGTTCTTAAACTCGATTCCAATTTCCCTGTTCCTCAAATCAACCAACACCAGGTCCTCGTCAAGGTCGTTGCTGCATCTCTCAACCCCATTGATTACAAGAGAATCCATGGAATGTTGAAACCTTCTAACTACTCCCCTCCGGTCAGTTATCATTCTCTCTCCCTCTCTTTTTCTTTTGATTTTACATGGGTTGTTTTGTTTTGATCTGAGCTTTTCTGGGTTTGTTCTTGATTTTGCATGCACGATACCATCTGATCTGGGTTGAGTTAAGGTCTTTTTCATCAGAACATATTGTGTAGTTCTTTCTGCCGTTTCTCATATGATCCTGAAGAAATTCATCTAACACCCTGTTGCAAACATAGAATCCTCTGCAGTAATGAGAATATCTTGGTATATCTGGAATCATAACATCTTTGAGATTTTAGACTGAATCTCAAAGTCAAACCATACGGATTCATATCATATCGTTTTGTTGAACAACAAAAGAAAATGTGGATTAGCAATTGAATGGATGCAAAATCAGGGTGGCTTATTGAGATGATTTTGGTTTCTCTAATTAATGAGATTGTGGGGAAGTTTGGGAACTAACATATTATGTGTCTTCTTCTTCCTGTAATTGATTTGTTCTTACAGTCAGTACCTGGCTATGATGTTGCTGGTGTGGTGGTGAAGGTGGGAAGTGAGGTGAAAAAGTTCAAGGTAGGAGACGAAGTATATGGAGATATAAATCACAAAGCACTAGATAAGCCAAAGAACGTTGGCACTTTGGCAGAATATACTGCTACAGAAGAGAAAGTTTTGGCTCTGAAGCCTAACAATCTGAGTTTCTTAGAGGCTGCCAGCCTTCCCCTTGCCATGGAAACTGCCTATGAAGGTCTCGAACGGGTTGGGTTCTCTGCTGGAAAATCGATCCTTGTTTTGGGAGGGGCTGGCGGAGTCGGAGCTTATGTTATACAGGTAAATGATATCTGTGCTGCTACACTCATTGTCTAATAAGTTTTGTTCTTCTTCATTGCTGGCTAATGTGATCTTGGTGTTTCTGTATGGACAGCTCGCAAAGCACGTGTTTGGTGCGTCGAGAGTTGTTGCTACTGCAAGCACATCAAAACAGGAATTCTTGAGTTGCTTGGGAGCTGATCAGCCTATTGATTACACAAAGGAAAACTTCGAAGACCTTCCCGAGAAATTCGATGTAGTCTACGATGCAGTCGGTAATAGTTACACTTCTTCACCTGGTTGTTAGTCTATGAAAAAATGTCTACAAATGATCCATGTCTTGATTTTCAAACTGCAGGCCAGCCCGATAAAGCCGTTAAGGCGATCAAAGAAGGTGGGAATATCGTAAGCATTGCTGCACCAGTTGCAGAATTTCCAGATGCTTTCTTCCTTCTTACTTCAGATGCTGCCATGTTGGAGAAACTGAAACCTTACCTAGAAAATGGAAAGGTGAAGCCGATCATCGACCCCAAATCTCCATTCCCATTCTCCGACACTCTCGATGCATTCGCCTATCTTGAAACCTCCCGAGCTACTGGGAAAGTTATGGTTTATCCCATCCCTTAGAAGCAAGTTTCTTAATCAGTTTTCTGTCATAATGCACCGGAGAAGAATCCTTGTTGTGTTTGGGAAAATAAGTTGTGTAAAATCTGCACATCATGTTTCTTGTTCATCTTGCTTAATGTACATTGTTGTGATGTTTGCTGTTTTAGAGCTGGAACGAAATAGCTTTTGACCATAATTTTGAGAGTTTTATTTAAATTTGCAATGCATAAACACATTCTTCTCCATATTTATTACAAACTTATCATATGATTTATGA

mRNA sequence

ATCCCATCCATGGCTGCTCCTTCTGTTCCCACCCACATGAAGGCTTGGGTCTACCATGATTTCGGCAACACTGCCGATGTTCTTAAACTCGATTCCAATTTCCCTGTTCCTCAAATCAACCAACACCAGGTCCTCGTCAAGGTCGTTGCTGCATCTCTCAACCCCATTGATTACAAGAGAATCCATGGAATGTTGAAACCTTCTAACTACTCCCCTCCGTCAGTACCTGGCTATGATGTTGCTGGTGTGGTGGTGAAGGTGGGAAGTGAGGTGAAAAAGTTCAAGGTAGGAGACGAAGTATATGGAGATATAAATCACAAAGCACTAGATAAGCCAAAGAACGTTGGCACTTTGGCAGAATATACTGCTACAGAAGAGAAAGTTTTGGCTCTGAAGCCTAACAATCTGAGTTTCTTAGAGGCTGCCAGCCTTCCCCTTGCCATGGAAACTGCCTATGAAGGTCTCGAACGGGTTGGGTTCTCTGCTGGAAAATCGATCCTTGTTTTGGGAGGGGCTGGCGGAGTCGGAGCTTATGTTATACAGCTCGCAAAGCACGTGTTTGGTGCGTCGAGAGTTGTTGCTACTGCAAGCACATCAAAACAGGAATTCTTGAGTTGCTTGGGAGCTGATCAGCCTATTGATTACACAAAGGAAAACTTCGAAGACCTTCCCGAGAAATTCGATGTAGTCTACGATGCAGTCGGCCAGCCCGATAAAGCCGTTAAGGCGATCAAAGAAGGTGGGAATATCGTAAGCATTGCTGCACCAGTTGCAGAATTTCCAGATGCTTTCTTCCTTCTTACTTCAGATGCTGCCATGTTGGAGAAACTGAAACCTTACCTAGAAAATGGAAAGGTGAAGCCGATCATCGACCCCAAATCTCCATTCCCATTCTCCGACACTCTCGATGCATTCGCCTATCTTGAAACCTCCCGAGCTACTGGGAAAGTTATGGTTTATCCCATCCCTTAGAAGCAAGTTTCTTAATCAGTTTTCTGTCATAATGCACCGGAGAAGAATCCTTGTTGTGTTTGGGAAAATAAGTTGTGTAAAATCTGCACATCATGTTTCTTGTTCATCTTGCTTAATGTACATTGTTGTGATGTTTGCTGTTTTAGAGCTGGAACGAAATAGCTTTTGACCATAATTTTGAGAGTTTTATTTAAATTTGCAATGCATAAACACATTCTTCTCCATATTTATTACAAACTTATCATATGATTTATGA

Coding sequence (CDS)

ATGGCTGCTCCTTCTGTTCCCACCCACATGAAGGCTTGGGTCTACCATGATTTCGGCAACACTGCCGATGTTCTTAAACTCGATTCCAATTTCCCTGTTCCTCAAATCAACCAACACCAGGTCCTCGTCAAGGTCGTTGCTGCATCTCTCAACCCCATTGATTACAAGAGAATCCATGGAATGTTGAAACCTTCTAACTACTCCCCTCCGTCAGTACCTGGCTATGATGTTGCTGGTGTGGTGGTGAAGGTGGGAAGTGAGGTGAAAAAGTTCAAGGTAGGAGACGAAGTATATGGAGATATAAATCACAAAGCACTAGATAAGCCAAAGAACGTTGGCACTTTGGCAGAATATACTGCTACAGAAGAGAAAGTTTTGGCTCTGAAGCCTAACAATCTGAGTTTCTTAGAGGCTGCCAGCCTTCCCCTTGCCATGGAAACTGCCTATGAAGGTCTCGAACGGGTTGGGTTCTCTGCTGGAAAATCGATCCTTGTTTTGGGAGGGGCTGGCGGAGTCGGAGCTTATGTTATACAGCTCGCAAAGCACGTGTTTGGTGCGTCGAGAGTTGTTGCTACTGCAAGCACATCAAAACAGGAATTCTTGAGTTGCTTGGGAGCTGATCAGCCTATTGATTACACAAAGGAAAACTTCGAAGACCTTCCCGAGAAATTCGATGTAGTCTACGATGCAGTCGGCCAGCCCGATAAAGCCGTTAAGGCGATCAAAGAAGGTGGGAATATCGTAAGCATTGCTGCACCAGTTGCAGAATTTCCAGATGCTTTCTTCCTTCTTACTTCAGATGCTGCCATGTTGGAGAAACTGAAACCTTACCTAGAAAATGGAAAGGTGAAGCCGATCATCGACCCCAAATCTCCATTCCCATTCTCCGACACTCTCGATGCATTCGCCTATCTTGAAACCTCCCGAGCTACTGGGAAAGTTATGGTTTATCCCATCCCTTAG

Protein sequence

MAAPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHGMLKPSNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTATEEKVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLAKHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKAIKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLDAFAYLETSRATGKVMVYPIP

Homology

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BLAST of Carg04428 vs. NCBI nr
Match: XP_022961926.1 (2-methylene-furan-3-one reductase-like [Cucurbita moschata] >KAG6602443.1 2-methylene-furan-3-one reductase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033125.1 2-methylene-furan-3-one reductase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 634.0 bits (1634), Expect = 6.9e-178
Identity = 320/320 (100.00%), Postives = 320/320 (100.00%), Query Frame = 0


Query: 1   MAAPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHG 60
           MAAPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHG
Sbjct: 1   MAAPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHG 60

Query: 61  MLKPSNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120
           MLKPSNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA
Sbjct: 61  MLKPSNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEEKVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180
           TEEKVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA
Sbjct: 121 TEEKVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180

Query: 181 KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240
           KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA
Sbjct: 181 KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240

Query: 241 IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLD 300
           IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLD
Sbjct: 241 IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLD 300

Query: 301 AFAYLETSRATGKVMVYPIP 321
           AFAYLETSRATGKVMVYPIP
Sbjct: 301 AFAYLETSRATGKVMVYPIP 320

[Close]

BLAST of Carg04428 vs. NCBI nr
Match: XP_022990587.1 (2-methylene-furan-3-one reductase-like [Cucurbita maxima])

HSP 1 Score: 625.5 bits (1612), Expect = 2.4e-175
Identity = 316/320 (98.75%), Postives = 317/320 (99.06%), Query Frame = 0


Query: 1   MAAPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHG 60
           MA PSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHG
Sbjct: 1   MAVPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHG 60

Query: 61  MLKPSNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120
           +LK SNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA
Sbjct: 61  ILKASNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEEKVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180
           TEEKVLALKP NLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA
Sbjct: 121 TEEKVLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180

Query: 181 KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240
           KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA
Sbjct: 181 KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240

Query: 241 IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLD 300
           IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLD
Sbjct: 241 IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLD 300

Query: 301 AFAYLETSRATGKVMVYPIP 321
           AFAYLETSRATGKVMVYPIP
Sbjct: 301 AFAYLETSRATGKVMVYPIP 320

[Close]

BLAST of Carg04428 vs. NCBI nr
Match: XP_023543317.1 (2-methylene-furan-3-one reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 618.6 bits (1594), Expect = 3.0e-173
Identity = 314/320 (98.12%), Postives = 316/320 (98.75%), Query Frame = 0


Query: 1   MAAPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHG 60
           MAAPSVPTHMKAWVYHDFGNTADVLKLDS+FPVPQINQHQVLVKVVAASLNPIDYKRIHG
Sbjct: 1   MAAPSVPTHMKAWVYHDFGNTADVLKLDSSFPVPQINQHQVLVKVVAASLNPIDYKRIHG 60

Query: 61  MLKPSNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120
            LK SNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA
Sbjct: 61  SLKASNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEEKVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180
           TEE VLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA
Sbjct: 121 TEETVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180

Query: 181 KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240
           KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQP+KAVKA
Sbjct: 181 KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPNKAVKA 240

Query: 241 IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLD 300
           IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPF FSDTLD
Sbjct: 241 IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFLFSDTLD 300

Query: 301 AFAYLETSRATGKVMVYPIP 321
           AFAYLETSRATGKVMVYPIP
Sbjct: 301 AFAYLETSRATGKVMVYPIP 320

[Close]

BLAST of Carg04428 vs. NCBI nr
Match: XP_038890251.1 (2-methylene-furan-3-one reductase-like [Benincasa hispida])

HSP 1 Score: 568.2 bits (1463), Expect = 4.6e-158
Identity = 283/320 (88.44%), Postives = 302/320 (94.38%), Query Frame = 0


Query: 1   MAAPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHG 60
           MAA SVPTHMKAWVYH+FGNTADVLKLDSN+PVP+IN++Q+L+KVVAASLNPIDYKRIHG
Sbjct: 1   MAALSVPTHMKAWVYHEFGNTADVLKLDSNYPVPRINENQLLIKVVAASLNPIDYKRIHG 60

Query: 61  MLKPSNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120
            LK  +YSPP VPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA
Sbjct: 61  ALKAFDYSPPYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEEKVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180
           TEE+VLALKP NLSFLEAASLPLAMETAYEGLER G SAGKSI+VLGGAGGVGAYVIQLA
Sbjct: 121 TEERVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSAGKSIIVLGGAGGVGAYVIQLA 180

Query: 181 KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240
           KHVFGAS+VVATASTSKQ+ L  LGADQPIDYTKENFEDL EKFD+VYDAVGQPDKAVKA
Sbjct: 181 KHVFGASKVVATASTSKQDLLRSLGADQPIDYTKENFEDLDEKFDIVYDAVGQPDKAVKA 240

Query: 241 IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLD 300
           +KEGGN+VSIA PV  FPDAFFLLTSDA MLEKL PYLE+GKVKPIID KSPFPFSDTLD
Sbjct: 241 LKEGGNLVSIATPVPGFPDAFFLLTSDAVMLEKLNPYLESGKVKPIIDSKSPFPFSDTLD 300

Query: 301 AFAYLETSRATGKVMVYPIP 321
           AFAYLETSRATGK+++YPIP
Sbjct: 301 AFAYLETSRATGKIVIYPIP 320

[Close]

BLAST of Carg04428 vs. NCBI nr
Match: XP_022938615.1 (2-methylene-furan-3-one reductase-like [Cucurbita moschata])

HSP 1 Score: 565.5 bits (1456), Expect = 3.0e-157
Identity = 281/320 (87.81%), Postives = 304/320 (95.00%), Query Frame = 0


Query: 1   MAAPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHG 60
           MAA SVPTHMKAWVYH+FGNTADVLKLD+N+PVP+IN++QVLVKVVAASLNPIDYKRIHG
Sbjct: 1   MAALSVPTHMKAWVYHEFGNTADVLKLDTNYPVPEINENQVLVKVVAASLNPIDYKRIHG 60

Query: 61  MLKPSNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120
            LK  NY PPS+PGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA
Sbjct: 61  SLKALNYLPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEEKVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180
           TE+KVLALKP NLSFLEAASLPLAMETAYEGLER G SAGKSI+VLGGAGGVGA+VIQLA
Sbjct: 121 TEDKVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSAGKSIIVLGGAGGVGAHVIQLA 180

Query: 181 KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240
           K+VFGAS++ ATASTSKQ+ L  LGADQPIDYTKENFEDLPEKFDVVYDAVGQ DKA+KA
Sbjct: 181 KNVFGASKIAATASTSKQDLLRSLGADQPIDYTKENFEDLPEKFDVVYDAVGQADKAIKA 240

Query: 241 IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLD 300
           +KEGG++VSIAAPVA FPDAFFLLTSDA +LEKL PYLE+GKVKPIIDPKSPFPFSDTL+
Sbjct: 241 MKEGGHLVSIAAPVAGFPDAFFLLTSDAVILEKLNPYLESGKVKPIIDPKSPFPFSDTLE 300

Query: 301 AFAYLETSRATGKVMVYPIP 321
           AFAYLETSRATGK++VYPIP
Sbjct: 301 AFAYLETSRATGKIVVYPIP 320

[Close]

BLAST of Carg04428 vs. ExPASy Swiss-Prot
Match: Q84V25 (2-methylene-furan-3-one reductase OS=Fragaria ananassa OX=3747 GN=EO PE=1 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 3.4e-119
Identity = 218/321 (67.91%), Postives = 261/321 (81.31%), Query Frame = 0


Query: 2   AAP--SVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIH 61
           AAP  S+P+  KAWVY ++G T+DVLK D +  VP++ + QVL+KVVAASLNP+D+KR  
Sbjct: 3   AAPSESIPSVNKAWVYSEYGKTSDVLKFDPSVAVPEVKEDQVLIKVVAASLNPVDFKRAL 62

Query: 62  GMLKPSNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYT 121
           G  K ++   P+VPGYDVAGVVVKVGS+V KFKVGDEVYGD+N  AL  P   G+LAEYT
Sbjct: 63  GYFKDTDSPLPTVPGYDVAGVVVKVGSQVTKFKVGDEVYGDLNEAALVNPTRFGSLAEYT 122

Query: 122 ATEEKVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQL 181
           A +E+VLA KP +LSF+EAASLPLA+ETAYEGLER   SAGKSILVLGGAGGVG ++IQL
Sbjct: 123 AADERVLAHKPKDLSFIEAASLPLAIETAYEGLERAELSAGKSILVLGGAGGVGTHIIQL 182

Query: 182 AKHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVK 241
           AKHVFGAS+V ATAST K +FL  LG D  IDYTKEN EDLPEKFDVVYDAVG+ DKAVK
Sbjct: 183 AKHVFGASKVAATASTKKLDFLRTLGVDLAIDYTKENIEDLPEKFDVVYDAVGETDKAVK 242

Query: 242 AIKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTL 301
           A+KEGG +V+I  P A  P   F+LTS  ++LEKLKPYLE+GKVKP++DP SP+PF+  +
Sbjct: 243 AVKEGGKVVTIVGP-ATPPAIHFVLTSKGSVLEKLKPYLESGKVKPVLDPTSPYPFTKLV 302

Query: 302 DAFAYLETSRATGKVMVYPIP 321
           +AF YLE+SRATGKV+VYPIP
Sbjct: 303 EAFGYLESSRATGKVVVYPIP 322

[Close]

BLAST of Carg04428 vs. ExPASy Swiss-Prot
Match: Q941I0 (2-methylene-furan-3-one reductase OS=Fragaria ananassa OX=3747 GN=EO PE=1 SV=2)

HSP 1 Score: 425.2 bits (1092), Expect = 6.3e-118
Identity = 218/322 (67.70%), Postives = 261/322 (81.06%), Query Frame = 0


Query: 2   AAP--SVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIH 61
           AAP  S+P+  KAWVY ++G T+DVLK D +  VP+I + QVL+KVVAASLNP+D+KR  
Sbjct: 3   AAPSESIPSVNKAWVYSEYGKTSDVLKFDPSVAVPEIKEDQVLIKVVAASLNPVDFKRAL 62

Query: 62  GMLKPSNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYT 121
           G  K ++   P++PGYDVAGVVVKVGS+V KFKVGDEVYGD+N  AL  P   G+LAEYT
Sbjct: 63  GYFKDTDSPLPTIPGYDVAGVVVKVGSQVTKFKVGDEVYGDLNETALVNPTRFGSLAEYT 122

Query: 122 ATEEKVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQL 181
           A  E+VLA KP NLSF+EAASLPLA+ETA+EGLER   SAGKS+LVLGGAGGVG ++IQL
Sbjct: 123 AAYERVLAHKPKNLSFIEAASLPLAIETAHEGLERAELSAGKSVLVLGGAGGVGTHIIQL 182

Query: 182 AKHVFGASRVVATASTSKQEFLSCLG-ADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAV 241
           AKHVFGAS+V ATAST K + L  LG AD  IDYTKENFEDLPEKFDVVYDAVG+ DKAV
Sbjct: 183 AKHVFGASKVAATASTKKLDLLRTLGAADLAIDYTKENFEDLPEKFDVVYDAVGETDKAV 242

Query: 242 KAIKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDT 301
           KA+KEGG +V+I  P A  P   F+LTS  ++LEKLKPYLE+GKVKP++DP SP+PF+  
Sbjct: 243 KAVKEGGKVVTIVGP-ATPPAILFVLTSKGSVLEKLKPYLESGKVKPVLDPTSPYPFTKV 302

Query: 302 LDAFAYLETSRATGKVMVYPIP 321
           ++AF YLE+SRATGKV+VYPIP
Sbjct: 303 VEAFGYLESSRATGKVVVYPIP 323

[Close]

BLAST of Carg04428 vs. ExPASy Swiss-Prot
Match: O23939 (2-methylene-furan-3-one reductase OS=Fragaria vesca OX=57918 GN=EO PE=2 SV=2)

HSP 1 Score: 424.9 bits (1091), Expect = 8.3e-118
Identity = 216/321 (67.29%), Postives = 260/321 (81.00%), Query Frame = 0


Query: 2   AAP--SVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIH 61
           AAP  S+P+  KAWV  ++G T+DVLK D +  VP+I + QVL+KVVAASLNP+D+KR  
Sbjct: 3   AAPSESIPSVNKAWVXSEYGKTSDVLKFDPSVAVPEIKEDQVLIKVVAASLNPVDFKRAL 62

Query: 62  GMLKPSNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYT 121
           G  K ++   P++PGY VAGVVVKVGS+V KFKVGDEVYGD+N  AL  P   G+LAEYT
Sbjct: 63  GYFKDTDSPLPTIPGYYVAGVVVKVGSQVTKFKVGDEVYGDLNETALVNPTRFGSLAEYT 122

Query: 122 ATEEKVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQL 181
           A +E+VLA KP NLSF+EAASLPLA+ETA+EGLER   SAGKS+LVLGGAGGVG ++IQL
Sbjct: 123 AADERVLAHKPKNLSFIEAASLPLAIETAHEGLERAELSAGKSVLVLGGAGGVGTHIIQL 182

Query: 182 AKHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVK 241
           AKHVFGAS+V ATAST K + L  LGAD  IDYTKENFEDLPEKFDVVYDAVG+ DKAVK
Sbjct: 183 AKHVFGASKVAATASTKKLDLLRTLGADLAIDYTKENFEDLPEKFDVVYDAVGETDKAVK 242

Query: 242 AIKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTL 301
           A+KEGG +V+I  P A  P   F+LTS  ++LEKLKPYLE+GKVKP++DP SP+PF+  +
Sbjct: 243 AVKEGGKVVTIVGP-ATPPAILFVLTSKGSVLEKLKPYLESGKVKPVLDPTSPYPFTKVV 302

Query: 302 DAFAYLETSRATGKVMVYPIP 321
           +AF YLE+SRATGKV+VYPIP
Sbjct: 303 EAFGYLESSRATGKVVVYPIP 322

[Close]

BLAST of Carg04428 vs. ExPASy Swiss-Prot
Match: K4BW79 (2-methylene-furan-3-one reductase OS=Solanum lycopersicum OX=4081 GN=EO PE=1 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 7.0e-117
Identity = 213/316 (67.41%), Postives = 257/316 (81.33%), Query Frame = 0


Query: 5   SVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHGMLKP 64
           S+P+ MKAW Y D+G + DVLKL+SN  VP I + QVL+K+VAA+LNP+D+KR  G  K 
Sbjct: 75  SIPSEMKAWSYTDYG-SVDVLKLESNVAVPDIKEDQVLIKIVAAALNPVDFKRRLGKFKA 134

Query: 65  SNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTATEEK 124
           ++   P+VPGYDVAGVVVKVGS+VK  K GDEVYGDI+ KALD PK  G+LAEYTA EEK
Sbjct: 135 TDSPLPTVPGYDVAGVVVKVGSQVKGLKEGDEVYGDIHEKALDGPKQFGSLAEYTAVEEK 194

Query: 125 VLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLAKHVF 184
           ++ALKP NLSF EAA+LPLA+ETAYEGLE+ GFS+GKSILVLGGAGGVG+ VIQLAKHVF
Sbjct: 195 LVALKPKNLSFAEAAALPLAIETAYEGLEKAGFSSGKSILVLGGAGGVGSLVIQLAKHVF 254

Query: 185 GASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKAIKEG 244
           GAS+V AT+ST K E L  LGAD  IDYTKENFEDLP+KFDVVYD+VGQ +KAVK +KEG
Sbjct: 255 GASKVAATSSTGKLELLKSLGADLAIDYTKENFEDLPDKFDVVYDSVGQGEKAVKVVKEG 314

Query: 245 GNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLDAFAY 304
           G++V +   V   P   F++TS+  ML+KL PYLE+GKVKP+IDPK PF F   +DAF+Y
Sbjct: 315 GSVVVLTGAVTP-PGFRFVVTSNGEMLKKLNPYLESGKVKPVIDPKGPFSFDKVVDAFSY 374

Query: 305 LETSRATGKVMVYPIP 321
           LET RATGKV+++PIP
Sbjct: 375 LETGRATGKVVIHPIP 388

[Close]

BLAST of Carg04428 vs. ExPASy Swiss-Prot
Match: Q9ZUC1 (NADPH-dependent alkenal/one oxidoreductase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AOR PE=1 SV=2)

HSP 1 Score: 400.6 bits (1028), Expect = 1.7e-110
Identity = 205/319 (64.26%), Postives = 248/319 (77.74%), Query Frame = 0


Query: 2   AAPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHGM 61
           A  S+P  MKAWVY D+G   DVLKL+SN  VP+I + QVL+KVVAA+LNP+D KR  G 
Sbjct: 70  ADASIPKEMKAWVYSDYGG-VDVLKLESNIVVPEIKEDQVLIKVVAAALNPVDAKRRQGK 129

Query: 62  LKPSNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTAT 121
            K ++   P+VPGYDVAGVVVKVGS VK  K GDEVY +++ KAL+ PK  G+LAEYTA 
Sbjct: 130 FKATDSPLPTVPGYDVAGVVVKVGSAVKDLKEGDEVYANVSEKALEGPKQFGSLAEYTAV 189

Query: 122 EEKVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLAK 181
           EEK+LALKP N+ F +AA LPLA+ETA EGL R  FSAGKSILVL GAGGVG+ VIQLAK
Sbjct: 190 EEKLLALKPKNIDFAQAAGLPLAIETADEGLVRTEFSAGKSILVLNGAGGVGSLVIQLAK 249

Query: 182 HVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKAI 241
           HV+GAS+V ATAST K E +  LGAD  IDYTKEN EDLP+K+DVV+DA+G  DKAVK I
Sbjct: 250 HVYGASKVAATASTEKLELVRSLGADLAIDYTKENIEDLPDKYDVVFDAIGMCDKAVKVI 309

Query: 242 KEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLDA 301
           KEGG +V++   V   P   F++TS+  +L+KL PY+E+GKVKP++DPK PFPFS   DA
Sbjct: 310 KEGGKVVALTGAVTP-PGFRFVVTSNGDVLKKLNPYIESGKVKPVVDPKGPFPFSRVADA 369

Query: 302 FAYLETSRATGKVMVYPIP 321
           F+YLET+ ATGKV+VYPIP
Sbjct: 370 FSYLETNHATGKVVVYPIP 386

[Close]

BLAST of Carg04428 vs. ExPASy TrEMBL
Match: A0A6J1HBQ4 (2-methylene-furan-3-one reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111462548 PE=3 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 3.3e-178
Identity = 320/320 (100.00%), Postives = 320/320 (100.00%), Query Frame = 0


Query: 1   MAAPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHG 60
           MAAPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHG
Sbjct: 1   MAAPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHG 60

Query: 61  MLKPSNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120
           MLKPSNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA
Sbjct: 61  MLKPSNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEEKVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180
           TEEKVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA
Sbjct: 121 TEEKVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180

Query: 181 KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240
           KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA
Sbjct: 181 KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240

Query: 241 IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLD 300
           IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLD
Sbjct: 241 IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLD 300

Query: 301 AFAYLETSRATGKVMVYPIP 321
           AFAYLETSRATGKVMVYPIP
Sbjct: 301 AFAYLETSRATGKVMVYPIP 320

[Close]

BLAST of Carg04428 vs. ExPASy TrEMBL
Match: A0A6J1JJ69 (2-methylene-furan-3-one reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111487421 PE=3 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 1.2e-175
Identity = 316/320 (98.75%), Postives = 317/320 (99.06%), Query Frame = 0


Query: 1   MAAPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHG 60
           MA PSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHG
Sbjct: 1   MAVPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHG 60

Query: 61  MLKPSNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120
           +LK SNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA
Sbjct: 61  ILKASNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEEKVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180
           TEEKVLALKP NLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA
Sbjct: 121 TEEKVLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180

Query: 181 KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240
           KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA
Sbjct: 181 KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240

Query: 241 IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLD 300
           IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLD
Sbjct: 241 IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLD 300

Query: 301 AFAYLETSRATGKVMVYPIP 321
           AFAYLETSRATGKVMVYPIP
Sbjct: 301 AFAYLETSRATGKVMVYPIP 320

[Close]

BLAST of Carg04428 vs. ExPASy TrEMBL
Match: A0A6J1FK96 (2-methylene-furan-3-one reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111444796 PE=3 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 1.5e-157
Identity = 281/320 (87.81%), Postives = 304/320 (95.00%), Query Frame = 0


Query: 1   MAAPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHG 60
           MAA SVPTHMKAWVYH+FGNTADVLKLD+N+PVP+IN++QVLVKVVAASLNPIDYKRIHG
Sbjct: 1   MAALSVPTHMKAWVYHEFGNTADVLKLDTNYPVPEINENQVLVKVVAASLNPIDYKRIHG 60

Query: 61  MLKPSNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120
            LK  NY PPS+PGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA
Sbjct: 61  SLKALNYLPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEEKVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180
           TE+KVLALKP NLSFLEAASLPLAMETAYEGLER G SAGKSI+VLGGAGGVGA+VIQLA
Sbjct: 121 TEDKVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSAGKSIIVLGGAGGVGAHVIQLA 180

Query: 181 KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240
           K+VFGAS++ ATASTSKQ+ L  LGADQPIDYTKENFEDLPEKFDVVYDAVGQ DKA+KA
Sbjct: 181 KNVFGASKIAATASTSKQDLLRSLGADQPIDYTKENFEDLPEKFDVVYDAVGQADKAIKA 240

Query: 241 IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLD 300
           +KEGG++VSIAAPVA FPDAFFLLTSDA +LEKL PYLE+GKVKPIIDPKSPFPFSDTL+
Sbjct: 241 MKEGGHLVSIAAPVAGFPDAFFLLTSDAVILEKLNPYLESGKVKPIIDPKSPFPFSDTLE 300

Query: 301 AFAYLETSRATGKVMVYPIP 321
           AFAYLETSRATGK++VYPIP
Sbjct: 301 AFAYLETSRATGKIVVYPIP 320

[Close]

BLAST of Carg04428 vs. ExPASy TrEMBL
Match: A0A6J1BXV5 (2-methylene-furan-3-one reductase-like OS=Momordica charantia OX=3673 GN=LOC111006288 PE=3 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 3.2e-157
Identity = 281/320 (87.81%), Postives = 303/320 (94.69%), Query Frame = 0


Query: 1   MAAPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHG 60
           MAA S+P  MKAWVYHDFGNTADVLKL+S++PVPQI+++QVLV+VVAASLNPID+KRIHG
Sbjct: 1   MAALSIPNQMKAWVYHDFGNTADVLKLESSYPVPQISENQVLVRVVAASLNPIDFKRIHG 60

Query: 61  MLKPSNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120
           +LK  NY+PPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA
Sbjct: 61  ILKAFNYNPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEEKVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180
           TE+KVLALKP NLSF+EAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGA+VIQLA
Sbjct: 121 TEDKVLALKPKNLSFIEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAHVIQLA 180

Query: 181 KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240
           KHVFGA+R+ ATAST+KQEFL  LGAD PIDYTK+NFEDLP+KFDVVYDAVGQPDKAVKA
Sbjct: 181 KHVFGAARIAATASTAKQEFLRSLGADLPIDYTKQNFEDLPDKFDVVYDAVGQPDKAVKA 240

Query: 241 IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLD 300
           IKEGG +VSIAAPV  FPDAFFLLTSDA MLEKL PYLENGKVKPIIDPKSPFPFSD L 
Sbjct: 241 IKEGGKLVSIAAPVPNFPDAFFLLTSDAVMLEKLNPYLENGKVKPIIDPKSPFPFSDVLQ 300

Query: 301 AFAYLETSRATGKVMVYPIP 321
           AFA+LETSRATGKV+VYPIP
Sbjct: 301 AFAHLETSRATGKVVVYPIP 320

[Close]

BLAST of Carg04428 vs. ExPASy TrEMBL
Match: A0A6J1JTD8 (2-methylene-furan-3-one reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111489564 PE=3 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 4.2e-157
Identity = 281/320 (87.81%), Postives = 303/320 (94.69%), Query Frame = 0


Query: 1   MAAPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHG 60
           MAA SVPTHMKAWVYH+FGNTADVLKLD+N+PVP+IN++QVLVKVVAASLNPIDYKRIHG
Sbjct: 1   MAALSVPTHMKAWVYHEFGNTADVLKLDTNYPVPEINENQVLVKVVAASLNPIDYKRIHG 60

Query: 61  MLKPSNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120
            LK  NY PPS+PGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA
Sbjct: 61  ALKALNYLPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEEKVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180
           TE+KVLALKP NLSFLEAASLPLAMETAYEGLER G SAGKSI+VLGGAGGVGA+VIQLA
Sbjct: 121 TEDKVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSAGKSIIVLGGAGGVGAHVIQLA 180

Query: 181 KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240
           K+VFGAS++ ATASTSKQ+ L  LGADQPIDYTKE+FEDLPEKFDVVYDAVGQ DKA+KA
Sbjct: 181 KNVFGASKIAATASTSKQDLLRSLGADQPIDYTKESFEDLPEKFDVVYDAVGQADKAIKA 240

Query: 241 IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLD 300
           +KEGG++VSIAAPVA FPDAFFLLTSDA MLEKL PYLE+GKVKPIIDPKSPFPFSDTL 
Sbjct: 241 MKEGGHLVSIAAPVAGFPDAFFLLTSDAVMLEKLNPYLESGKVKPIIDPKSPFPFSDTLK 300

Query: 301 AFAYLETSRATGKVMVYPIP 321
           AFAYLETSRATGK++VYPIP
Sbjct: 301 AFAYLETSRATGKIVVYPIP 320

[Close]

BLAST of Carg04428 vs. TAIR 10
Match: AT1G23740.1 (Oxidoreductase, zinc-binding dehydrogenase family protein )

HSP 1 Score: 400.6 bits (1028), Expect = 1.2e-111
Identity = 205/319 (64.26%), Postives = 248/319 (77.74%), Query Frame = 0


Query: 2   AAPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHGM 61
           A  S+P  MKAWVY D+G   DVLKL+SN  VP+I + QVL+KVVAA+LNP+D KR  G 
Sbjct: 70  ADASIPKEMKAWVYSDYGG-VDVLKLESNIVVPEIKEDQVLIKVVAAALNPVDAKRRQGK 129

Query: 62  LKPSNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTAT 121
            K ++   P+VPGYDVAGVVVKVGS VK  K GDEVY +++ KAL+ PK  G+LAEYTA 
Sbjct: 130 FKATDSPLPTVPGYDVAGVVVKVGSAVKDLKEGDEVYANVSEKALEGPKQFGSLAEYTAV 189

Query: 122 EEKVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLAK 181
           EEK+LALKP N+ F +AA LPLA+ETA EGL R  FSAGKSILVL GAGGVG+ VIQLAK
Sbjct: 190 EEKLLALKPKNIDFAQAAGLPLAIETADEGLVRTEFSAGKSILVLNGAGGVGSLVIQLAK 249

Query: 182 HVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKAI 241
           HV+GAS+V ATAST K E +  LGAD  IDYTKEN EDLP+K+DVV+DA+G  DKAVK I
Sbjct: 250 HVYGASKVAATASTEKLELVRSLGADLAIDYTKENIEDLPDKYDVVFDAIGMCDKAVKVI 309

Query: 242 KEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLDA 301
           KEGG +V++   V   P   F++TS+  +L+KL PY+E+GKVKP++DPK PFPFS   DA
Sbjct: 310 KEGGKVVALTGAVTP-PGFRFVVTSNGDVLKKLNPYIESGKVKPVVDPKGPFPFSRVADA 369

Query: 302 FAYLETSRATGKVMVYPIP 321
           F+YLET+ ATGKV+VYPIP
Sbjct: 370 FSYLETNHATGKVVVYPIP 386

[Close]

BLAST of Carg04428 vs. TAIR 10
Match: AT4G13010.1 (Oxidoreductase, zinc-binding dehydrogenase family protein )

HSP 1 Score: 141.0 bits (354), Expect = 1.7e-33
Identity = 118/337 (35.01%), Postives = 162/337 (48.07%), Query Frame = 0


Query: 10  MKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHGMLKPSNYSP 69
           M A  Y+ +G  A  L+     PVP    ++V +K+ A SLNP+D+K   GM++P  + P
Sbjct: 6   MHALQYNSYGGGAAGLE-HVQVPVPTPKSNEVCLKLEATSLNPVDWKIQKGMIRP--FLP 65

Query: 70  ---PSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTATEEKVL 129
              P +P  DVAG VV+VGS VK FK GD+V   ++H         G LAE+    EK+ 
Sbjct: 66  RKFPCIPATDVAGEVVEVGSGVKNFKAGDKVVAVLSH------LGGGGLAEFAVATEKLT 125

Query: 130 ALKPNNLSFLEAASLPLAMETAYE------GLERVGFSAGKSILVLGGAGGVGAYVIQLA 189
             +P  +   EAA+LP+A  TA +      GL+  G     +ILV   +GGVG Y +QLA
Sbjct: 126 VKRPQEVGAAEAAALPVAGLTALQALTNPAGLKLDGTGKKANILVTAASGGVGHYAVQLA 185

Query: 190 KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAV-----GQPD 249
           K     + V AT      EF+  LGAD+ +DY       L       YDAV     G P 
Sbjct: 186 K--LANAHVTATCGARNIEFVKSLGADEVLDYKTPEGAALKSPSGKKYDAVVHCANGIPF 245

Query: 250 KAVKA-IKEGGNIVSIA-APVAEFPDAF------------FLLTSDAAMLEKLKPYLENG 309
              +  + E G ++ I   P A +  A              LL   A  LE +   ++ G
Sbjct: 246 SVFEPNLSENGKVIDITPGPNAMWTYAVKKITMSKKQLVPLLLIPKAENLEFMVNLVKEG 305

Query: 310 KVKPIIDPKSPFPFSDTLDAFAYLETSRATGKVMVYP 319
           KVK +ID K   P S   DA+A      ATGK++V P
Sbjct: 306 KVKTVIDSK--HPLSKAEDAWAKSIDGHATGKIIVEP 329

[Close]

BLAST of Carg04428 vs. TAIR 10
Match: AT3G15090.1 (GroES-like zinc-binding alcohol dehydrogenase family protein )

HSP 1 Score: 136.7 bits (343), Expect = 3.2e-32
Identity = 107/349 (30.66%), Postives = 168/349 (48.14%), Query Frame = 0


Query: 5   SVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHGMLKP 64
           SV T  +A +   FG   +V +L  N PVP +N ++VLVK  A S+NP+D  RI      
Sbjct: 27  SVFTGCRAVILPRFGG-PEVFELRENVPVPNLNPNEVLVKAKAVSVNPLDC-RIRAGYGR 86

Query: 65  SNYSP--PSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTATE 124
           S + P  P + G DV+G V  +G+ VK  KVG EV+G ++  AL      GT  +Y    
Sbjct: 87  SVFQPHLPIIVGRDVSGEVAAIGTSVKSLKVGQEVFGALHPTALR-----GTYTDYGILS 146

Query: 125 EKVLALKPNNLSFLEAASLPLAMETAYEGLE-RVGFSAGKSILVLGGAGGVGAYVIQLAK 184
           E  L  KP+++S +EA+++P A  TA+  L+     + G+ +LV GG G VG   IQLA 
Sbjct: 147 EDELTEKPSSISHVEASAIPFAALTAWRALKSNARITEGQRLLVFGGGGAVGFSAIQLA- 206

Query: 185 HVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFE-DLPEKFDVVYDAVGQPDK---A 244
            V     V A+     ++ +   GA+Q +DYT E+ E  +  KFD V D +G P+     
Sbjct: 207 -VASGCHVTASCVGQTKDRILAAGAEQAVDYTTEDIELAVKGKFDAVLDTIGGPETERIG 266

Query: 245 VKAIKEGGNIVSIAAPVAEFPDAF------------------------------FLLTSD 304
           +  +++GGN +++    A   D +                                + +D
Sbjct: 267 INFLRKGGNYMTLQGEAASLTDKYGFVVGLPLATSLLMKKKIQYQYSHGIDYWWTYMRAD 326

Query: 305 AAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLDAFAYLETSRATGKVMV 317
              L +++  +  GK+K  I  +  FP +D + A    E  +  GKV++
Sbjct: 327 PEGLAEIQRLVGAGKLK--IPVEKTFPITDVVAAHEAKEKKQIPGKVVL 364

[Close]

BLAST of Carg04428 vs. TAIR 10
Match: AT3G56460.1 (GroES-like zinc-binding alcohol dehydrogenase family protein )

HSP 1 Score: 104.8 bits (260), Expect = 1.3e-22
Identity = 80/254 (31.50%), Postives = 125/254 (49.21%), Query Frame = 0


Query: 19  GNTADVLKLDSNFPVPQINQH-QVLVKVVAASLNPIDYKRIHGMLKPSNYSP-PSVPGYD 78
           G+    +++    P+P +N    V V+V+A SLN  +Y +I G  K     P P +PG D
Sbjct: 18  GSPESPVEVSKTHPIPSLNSDTSVRVRVIATSLNYANYLQILG--KYQEKPPLPFIPGSD 77

Query: 79  VAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTATEEKVLALKPNNLSFL 138
            +G+V  +G  V KF+VGD V             ++G+ A++   ++  L L P     +
Sbjct: 78  YSGIVDAIGPAVTKFRVGDRVCSF---------ADLGSFAQFIVADQSRLFLVPERCDMV 137

Query: 139 EAASLPLAMETAYEGL-ERVGFSAGKSILVLGGAGGVGAYVIQLAKHVFGASRVVATAST 198
            AA+LP+A  T++  L  R   ++G+ +LVLG AGGVG   +Q+ K V GA  +     T
Sbjct: 138 AAAALPVAFGTSHVALVHRARLTSGQVLLVLGAAGGVGLAAVQIGK-VCGAIVIAVARGT 197

Query: 199 SKQEFLSCLGADQPIDYTKENFEDLPEKF---------DVVYDAVG--QPDKAVKAIKEG 258
            K + L  +G D  +D   EN     ++F         DV+YD VG     +++K +K G
Sbjct: 198 EKIQLLKSMGVDHVVDLGTENVISSVKEFIKTRKLKGVDVLYDPVGGKLTKESMKVLKWG 257

[Close]

BLAST of Carg04428 vs. TAIR 10
Match: AT4G21580.1 (oxidoreductase, zinc-binding dehydrogenase family protein )

HSP 1 Score: 91.3 bits (225), Expect = 1.5e-18
Identity = 97/340 (28.53%), Postives = 158/340 (46.47%), Query Frame = 0


Query: 10  MKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHGMLKPSNYSP 69
           MKA V  + G   +VL+L  +   P++   +VL++V+A +LN  D  +  G+  P   S 
Sbjct: 1   MKAIVISEPGK-PEVLQL-RDVADPEVKDDEVLIRVLATALNRADTLQRLGLYNPPPGSS 60

Query: 70  PSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTATEEKVLALK 129
           P + G + +G +  VG  V ++KVGD+V   ++          G  AE  +     +   
Sbjct: 61  PYL-GLECSGTIESVGKGVSRWKVGDQVCALLSG---------GGYAEKVSVPAGQIFPI 120

Query: 130 PNNLSFLEAASLPLAMETAYEGLERVG-FSAGKSILVLGGAGGVGAYVIQLAKHVFGASR 189
           P  +S  +AA+ P    T +  +  +G  S G+S L+ GG+ G+G + IQ+AKH+    R
Sbjct: 121 PAGISLKDAAAFPEVACTVWSTVFMMGRLSVGESFLIHGGSSGIGTFAIQIAKHL--GVR 180

Query: 190 VVATASTSKQEFLSC--LGADQPIDYTKENF------EDLPEKFDVVYDAVGQP--DKAV 249
           V  TA  S ++  +C  LGAD  I+Y  E+F      E   +  DV+ D +G P   K +
Sbjct: 181 VFVTAG-SDEKLAACKELGADVCINYKTEDFVAKVKAETDGKGVDVILDCIGAPYLQKNL 240

Query: 250 KAIKEGGNIVSI---AAPVAEFPDAFFL---LTSDAAML----------------EKLKP 309
            ++   G +  I       AE   +  L   LT   A L                + + P
Sbjct: 241 DSLNFDGRLCIIGLMGGANAEIKLSSLLPKRLTVLGAALRPRSPENKAVVVREVEKNVWP 300

Query: 310 YLENGKVKPIIDPKSPFPFSDTLDAFAYLETSRATGKVMV 317
            +E GKVKP+I      P S   +  + +E+S   GK+++
Sbjct: 301 AIEAGKVKPVI--YKYLPLSQAAEGHSLMESSNHIGKILL 323

The following BLAST results are available for this feature:

BLAST of Carg04428 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022961926.1	6.9e-178	100.00	2-methylene-furan-3-one reductase-like [Cucurbita moschata] >KAG6602443.1 2-meth...	[more]
XP_022990587.1	2.4e-175	98.75	2-methylene-furan-3-one reductase-like [Cucurbita maxima]	[more]
XP_023543317.1	3.0e-173	98.13	2-methylene-furan-3-one reductase-like [Cucurbita pepo subsp. pepo]	[more]
XP_038890251.1	4.6e-158	88.44	2-methylene-furan-3-one reductase-like [Benincasa hispida]	[more]
XP_022938615.1	3.0e-157	87.81	2-methylene-furan-3-one reductase-like [Cucurbita moschata]	[more]

BLAST of Carg04428 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84V25	3.4e-119	67.91	2-methylene-furan-3-one reductase OS=Fragaria ananassa OX=3747 GN=EO PE=1 SV=1	[more]
Q941I0	6.3e-118	67.70	2-methylene-furan-3-one reductase OS=Fragaria ananassa OX=3747 GN=EO PE=1 SV=2	[more]
O23939	8.3e-118	67.29	2-methylene-furan-3-one reductase OS=Fragaria vesca OX=57918 GN=EO PE=2 SV=2	[more]
K4BW79	7.0e-117	67.41	2-methylene-furan-3-one reductase OS=Solanum lycopersicum OX=4081 GN=EO PE=1 SV=...	[more]
Q9ZUC1	1.7e-110	64.26	NADPH-dependent alkenal/one oxidoreductase, chloroplastic OS=Arabidopsis thalian...	[more]

BLAST of Carg04428 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HBQ4	3.3e-178	100.00	2-methylene-furan-3-one reductase-like OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A6J1JJ69	1.2e-175	98.75	2-methylene-furan-3-one reductase-like OS=Cucurbita maxima OX=3661 GN=LOC1114874...	[more]
A0A6J1FK96	1.5e-157	87.81	2-methylene-furan-3-one reductase-like OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1BXV5	3.2e-157	87.81	2-methylene-furan-3-one reductase-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1JTD8	4.2e-157	87.81	2-methylene-furan-3-one reductase-like OS=Cucurbita maxima OX=3661 GN=LOC1114895...	[more]

BLAST of Carg04428 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G23740.1	1.2e-111	64.26	Oxidoreductase, zinc-binding dehydrogenase family protein	[more]
AT4G13010.1	1.7e-33	35.01	Oxidoreductase, zinc-binding dehydrogenase family protein	[more]
AT3G15090.1	3.2e-32	30.66	GroES-like zinc-binding alcohol dehydrogenase family protein	[more]
AT3G56460.1	1.3e-22	31.50	GroES-like zinc-binding alcohol dehydrogenase family protein	[more]
AT4G21580.1	1.5e-18	28.53	oxidoreductase, zinc-binding dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020843	Polyketide synthase, enoylreductase domain	SMART	SM00829	PKS_ER_names_mod	coord: 20..316 e-value: 2.9E-13 score: 35.7
None	No IPR available	PFAM	PF13602	ADH_zinc_N_2	coord: 204..316 e-value: 4.8E-19 score: 69.7
None	No IPR available	GENE3D	3.40.50.720		coord: 152..267 e-value: 1.5E-106 score: 357.9
None	No IPR available	GENE3D	3.90.180.10		coord: 18..316 e-value: 1.5E-106 score: 357.9
None	No IPR available	PANTHER	PTHR44573:SF3	CYTOSOLIC ALKENAL/ONE OXIDOREDUCTASE	coord: 3..320
None	No IPR available	CDD	cd05289	MDR_like_2	coord: 10..316 e-value: 2.15578E-99 score: 293.313
IPR013154	Alcohol dehydrogenase, N-terminal	PFAM	PF08240	ADH_N	coord: 39..101 e-value: 7.4E-11 score: 41.9
IPR044626	NADPH-dependent oxidoreductase AOR-like	PANTHER	PTHR44573	NADPH-DEPENDENT ALKENAL/ONE OXIDOREDUCTASE, CHLOROPLASTIC	coord: 3..320
IPR002364	Quinone oxidoreductase/zeta-crystallin, conserved site	PROSITE	PS01162	QOR_ZETA_CRYSTAL	coord: 160..181
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 133..287
IPR011032	GroES-like superfamily	SUPERFAMILY	50129	GroES-like	coord: 6..167

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg04428-RA	Carg04428-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016628	oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0016491	oxidoreductase activity