Carg04423 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04423
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Folylpolyglutamate synthase
Location	Carg_Chr04: 20468650 .. 20473594 (+)
RNA-Seq Expression	Carg04423
Synteny	Carg04423

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAATGGAAGTTCGCAAGTTATCATTCACACCATGGCGCTGCTTTCGGCTGCTGATGAATCACCGCAGTAGTGACTTTCCAACGACATTGACTCGACCTCCACGCCACCCCACAACAACTTGAGCAACTCCTGCATTGTTATTGTTATTTGATCCATCCAATTCCTGTCCGCCCGAAACGATTCCTTTATCACCTGTTTCGACGGTCCAAGACATACCCTCTTCAGCTTATGCCACTCTCTTTCTCTAGCCGCCCCCTTCCAGTCTTCGGCACTCGCCCTCGGCTTCTCCGACGTTCCCGAAAGGAAGAGATTGGTGAAAAGGTGAGGACCCTTTTCCCCTTTTGACTCTGTTCTTATCTGAGCACTGAATCCTTGGTTATGGAATCTGTAATTATGTTTTGTTGAATATTACTCTGCTGCTTGTACGAGAAGGCGATGAAAATTGAACCCGTTTTTTCTTTATTGCCAATTAGTGTAAAGGAAACGGGGAAATTAGATACCCAATCGAATATATTCTTAATTAGAGCAGGGAATAAGCAGGGAATAAGCTAGACTATGAATGTAATATGTGTAAAGAAATTCTACCTCATCTTAAGCGATTAGGTAGCTTTTTTTTAGAGGGGGTTTGTTCCGTGAGCTATATGTTGGAACTTCCTGATTGTTTGATGTATTCTATGCTTTTCCCTGCATCTAAAAGACATTAGCTGTTTTGCAGCTTTGGAGAGTAGCAAACGTTGGGGGCCTTTGAATCTTTTAGACTAGGCTGAATCCTCAGGATATTGGAGAAGCAGGGGGTCATCTAGTAACCATGGAGGAAGGTATGGACACATTTTAAACCACTTTTGATGTGTTAAGCTATTCTATCATGTAAAACCAAAAAACGTTTTGGGGTTGTGATGGGTGCTTTTGAAGGTATGGGAGCATTGAAATACTCGCGGACAGCCTATGAAGAGGCATTGGATGTACTGTCCTCGTTAATTGTCAGCCGTAGACGCTGCGATAAATCTGGAGATCGGTTTGAGTTGCTGTTTGAGTATGTAAAGGTATTACTGAAACTTAACACTTTATTATTGCTATAGTATACTTGAGCTGATGATCATTGAGTTGACTCAATATAATAGATGCTTGACTTAGAGGAGTCCATGTCGAAAATGAAGGTCATCCATGTAGCTGGCACCAAAGGCAAGGTACCATCTTCATCAGTTCATGCTCTTTCATTTGGATACTTCTACTTTTGTCCCATTCACTTTAACATTGTCAACTTAGAATTTTCGAACTTTGCTTAGAAAACATCATTGAGTTAGCTTAAAAATGATGCTGTGTTAACTAAACTTCTTTCTTTTTTCGGGACTTACATAGGGATCCACGTGCACCTTTACCGAATCCATATTGCGTAAATGTGGCTTCCACACTGGCCTTTTCACTTCTCCTCACCTTATTGATGTCCGAGAAAGATTTCGCTTGGATGGGTAACTATTGTGCATACTACGTAGTTTTGTTGTTCTTTTACGTGGTAAAGTTGGTCGATCGCTACTTTGTTTATTGAGATGATGTAGATGGTTGTCCTTACAGTAAATCCATTGATTTTTCGTTGTATCAACGACTTGCTTCGTATTATCTAACTTTATTTCTGGAACTATTTTCTCTCTTCCCACTTTGAAGTTGCAAACTCTTGAATACCTTCTAATTGGTTGTTGCAGCTTCGACATCAGTGAAGAGAAGTTTTTAGCCTATTTCTGGTGGTGTTATAATAGGCTAAAGGTACAATTTGATGTGTTATTATTACTTTTAATACATAAGTTCATCTAATGGATAAAATATATGGTGTCTTTGAATCTAATTGTATATGTAAACATTATATTTAGATTCTGAGTTGCATATTCTTGTAGTAAAGAGACGTTTGAACTCTATAAAACTAAACTATGGGATTCATTATTATCTTTTAACTGTAAATAAAGCATTTCTTCGATATTTTGGAAGTTTTCTAGAACAATTTATTTTCTTCAGGAAAAAACTAGTGAGGATGTACCGATGCCTCACTTGTTTCGCTTCCTAGCGTTACTTGCGTTCAAAATTTTTGCAGATGAGCAGGTTTGATGTGTTCTTTTTATTTTAATCTTTTTCTGGATACGCTTTCTTTGGTCAATTTATTACTGGGATTATAATGATGATGTTTTTATTGGCATCAATGAAGGTGGATGTAGCTATCTTGGAGGTTGGTTTAGGTGGAACTTTTGATGCTACTAATGTGGTATGTTCATTCTCTTTTGGCTTAAATTATAAAGCATTTCAGGAGATCCCACATCGGTTGGAGAGGGGAACGAAGCATTCCTTATAAGGGTGTGGAAACCTTTCCCTAACAGACGCGATTTAAAACTGTGAGACTGATGGTGATACGTAACGGCCCAAAGTGGATAATATTTGCTAGCGGTGGGCTTGGGCTGTTACCGATATAATTTATTCTTCATTAGAAAGTAGAAATCTTCATCAAGGTATAGCCATTTCATAGATTCAAATGTTTCAGGTCCAGGCACCCATTGTGTGTGGTATAGCTTCTTTGGGTTACGACCACACGGAACTTCTTGGTTAGCCTTCTCTCGACCTTGACAATAGTTTTCCTGGAAAATAAATGTTCGACAACTGTTTGCGTTTATGGCTTAAGCACAAGCGTAGATGTTTTCTATCCTGTTTGATAAGTTTGATAATGTCAAGGTTTTCTTACATTGTTGCTTTCTTTCTGTTTTGAATTTGTCCAATTAGGAAACACTCTTGGTGCGATAGCTTCGGAGAAAGCTGGTATCTTCAAGGTTTGCCTCAAGAAACTTTCCTTAAAGCATGAACGTCTAGGAATAATCATTTCATTTTGTTTCCATCCTTTCACTTTGGTGCAGCGTGGGGCTCCAGCTTTTACTGTATGTCAACCTGATGAAGCCATGCATGTGCTTAGAGATAAAGCTTCCCAGTTGGATGTATGTGCAAAGCTCTTCCGTTAGCTACTTCATGACTATGTGTTGTTCACACTGCCTTATATTTGCACGTCCCAGGTACAACTTACAGTAGTGCCATCGTTGGATCCGGAGCTTCTACAAGGTCATAAACTCGGGCTTGAAGGCGAGCACCAGTATCTAAATGCTCGTCTTGCTATTGCGCTATCCTCCACTTGGCTTCACAGGACCGGCCATTCCAATCTGTCGTACAAGGAACAGAGTGTAAGTTCTACATATGGGATTTGTCATCAGTTTTCCGTAAACATATCTCGTGTTTTCTGATTCTTTTTCTTCTTAACAATATGTTACCAGGGGTCTTTGCCTGAGCACTTCATAGAAGGGCTTACCACTGCCAGCTTTGAAGGGCGAGCTCAGATTGTTCCTGATAGATATAATGATATTGAATCCCCGGAAGATCTTGTCTTTTATTTGGATGGTGCCCATAGTCCTGAGAGCATGGTGGTTTGCAGTAGCTGGTTTTGTGCTGCAATAAAACATAACCAGGAAGGGATCTTAGATCATCAGTTGGATGATACTGGTACATCTTCAAATAAACTTCTTCAAGGATATGATAATGATGAGGCAAGGAAGAAATCTGTGCAGGTAGATTTCGACTTTAGCTCGACTTCTCCAAGCTCGTTTTATTGTTTGTCAAGAAAAAGGGATCATAAATAAGAAAGCTAGTTTCAGTTTGAATACCTTTTCATTTATGCAGTGCTTCAACTTCTTTAGATGTTGTTTAATCTAGTAGTGTTGGACATTGTAGGTGCTATTGTTCAATTGTATGTCGGTACGAGATCCACAATTGCTTCTTCAACCTCTGATCAAAACGTGCGCAAATAATGGTATGTATTTGTCGAACTCTTCGAACTGAAAAAATCTGGTGATCTGGTTAATACACAGTATTTTTTTAGGATTTAAAAGAATGATCTAAATATAACATTACTCGGAAAATCGAAGCTATAGAATAAGTTTTTAGCTGATGGATTAACGTCTTACGTTGTGAGATCTCATTTTGGTTAGAGAGGGGAACAAAGCATTCCTTATAAGAATGTGGAAACTTCTCTCTAACAGATGCGTTTTAAAACCGTGAGGCTGACGGCGATACGTAATGGTTCAAAGTGGACAATATCTATTAGCATTGGGCTTGGATGTAACGTTAAATAAGATATGCCAGAAATCAATAAAATTCCGTAAAAGTTCGGTTATATGAACTCAGGGGTCCACTTCAAGAAGGCAGTCTTTGTTCCAAATACATCATTGTTCAACAAGGTTGGAACCCATGGTTTAGCAGAAGCAAATGGTGAAGTAGACCTTTCTTGGCAGTTCCATGTTCGAAGGGTGTGGGAAAACATCATCCAATCTGAGAAAGGTATTATCTGTTTATGCTAGTGATCTTTTTCTAAGTCTTTGGAACCATTCTTTATCCTTCCACATATTAGGAGGCGATTCTCAAGGCATTGGTGCCATGGTTGAAGAAGAAAGTGAAAAACCAGAAGTGAGTGGTAAGAGCTGCGAGAACAGCGCAGTGTTTTCTTCTCTCCCAATGGCCATTAAATGGCTCCGAGATAGTGTTCTGCAGCAGAACAGGTCCATTCGACTTCAGGTGCGAATATTTTCGAGTATGTAGACATGGGCACGTTTGGAACGAAGAAATTTACGAGCTTTCTATTATTCAGGTTCTTGTGACTGGTTCTTTACATCTTGTGGGAGATGTGTTGAAATTAATCAAGTGAAGGACGGGACGTGTTTAGCGTCCACGGCGATGGGACTACGCTTTTACTCGAACAACGACATCAGCAAGAACTCGGTGCTAGAATTCTTTGAACATTCATGGTCTCATATTTGTTGTTTACAAGACTTGAGGTAATAATGGCCTTCTTAGGCTTCCATTTTGATCAGGGTTACATTTATTCTCTTTTTCATTCATTATTCTTATTTCATTATTATAATATATA

mRNA sequence

TAATGGAAGTTCGCAAGTTATCATTCACACCATGGCGCTGCTTTCGGCTGCTGATGAATCACCGCAGTAGTGACTTTCCAACGACATTGACTCGACCTCCACGCCACCCCACAACAACTTGAGCAACTCCTGCATTGTTATTGTTATTTGATCCATCCAATTCCTGTCCGCCCGAAACGATTCCTTTATCACCTGTTTCGACGGTCCAAGACATACCCTCTTCAGCTTATGCCACTCTCTTTCTCTAGCCGCCCCCTTCCAGTCTTCGGCACTCGCCCTCGGCTTCTCCGACGTTCCCGAAAGGAAGAGATTGGTGAAAAGCTTTGGAGAGTAGCAAACGTTGGGGGCCTTTGAATCTTTTAGACTAGGCTGAATCCTCAGGATATTGGAGAAGCAGGGGGTCATCTAGTAACCATGGAGGAAGGTATGGGAGCATTGAAATACTCGCGGACAGCCTATGAAGAGGCATTGGATGTACTGTCCTCGTTAATTGTCAGCCGTAGACGCTGCGATAAATCTGGAGATCGGTTTGAGTTGCTGTTTGAGTATGTAAAGATGCTTGACTTAGAGGAGTCCATGTCGAAAATGAAGGTCATCCATGTAGCTGGCACCAAAGGCAAGGGATCCACGTGCACCTTTACCGAATCCATATTGCGTAAATGTGGCTTCCACACTGGCCTTTTCACTTCTCCTCACCTTATTGATGTCCGAGAAAGATTTCGCTTGGATGGCTTCGACATCAGTGAAGAGAAGTTTTTAGCCTATTTCTGGTGGTGTTATAATAGGCTAAAGGAAAAAACTAGTGAGGATGTACCGATGCCTCACTTGTTTCGCTTCCTAGCGTTACTTGCGTTCAAAATTTTTGCAGATGAGCAGGTGGATGTAGCTATCTTGGAGGTTGGTTTAGGTGGAACTTTTGATGCTACTAATGTGGTCCAGGCACCCATTGTGTGTGGTATAGCTTCTTTGGGTTACGACCACACGGAACTTCTTGGAAACACTCTTGGTGCGATAGCTTCGGAGAAAGCTGGTATCTTCAAGCGTGGGGCTCCAGCTTTTACTGTATGTCAACCTGATGAAGCCATGCATGTGCTTAGAGATAAAGCTTCCCAGTTGGATGTACAACTTACAGTAGTGCCATCGTTGGATCCGGAGCTTCTACAAGGTCATAAACTCGGGCTTGAAGGCGAGCACCAGTATCTAAATGCTCGTCTTGCTATTGCGCTATCCTCCACTTGGCTTCACAGGACCGGCCATTCCAATCTGTCGTACAAGGAACAGAGTGGGTCTTTGCCTGAGCACTTCATAGAAGGGCTTACCACTGCCAGCTTTGAAGGGCGAGCTCAGATTGTTCCTGATAGATATAATGATATTGAATCCCCGGAAGATCTTGTCTTTTATTTGGATGGTGCCCATAGTCCTGAGAGCATGGTGGTTTGCAGTAGCTGGTTTTGTGCTGCAATAAAACATAACCAGGAAGGGATCTTAGATCATCAGTTGGATGATACTGGTACATCTTCAAATAAACTTCTTCAAGGATATGATAATGATGAGGCAAGGAAGAAATCTGTGCAGGTGCTATTGTTCAATTGTATGTCGGTACGAGATCCACAATTGCTTCTTCAACCTCTGATCAAAACGTGCGCAAATAATGGGGTCCACTTCAAGAAGGCAGTCTTTGTTCCAAATACATCATTGTTCAACAAGGTTGGAACCCATGGTTTAGCAGAAGCAAATGGTGAAGTAGACCTTTCTTGGCAGTTCCATGTTCGAAGGGTGTGGGAAAACATCATCCAATCTGAGAAAGGAGGCGATTCTCAAGGCATTGGTGCCATGGTTGAAGAAGAAAGTGAAAAACCAGAAGTGAGTGGTAAGAGCTGCGAGAACAGCGCAGTGTTTTCTTCTCTCCCAATGGCCATTAAATGGCTCCGAGATAGTGTTCTGCAGCAGAACAGGTCCATTCGACTTCAGGTTCTTGTGACTGGTTCTTTACATCTTGTGGGAGATGTGTTGAAATTAATCAAGTGAAGGACGGGACGTGTTTAGCGTCCACGGCGATGGGACTACGCTTTTACTCGAACAACGACATCAGCAAGAACTCGGTGCTAGAATTCTTTGAACATTCATGGTCTCATATTTGTTGTTTACAAGACTTGAGGTAATAATGGCCTTCTTAGGCTTCCATTTTGATCAGGGTTACATTTATTCTCTTTTTCATTCATTATTCTTATTTCATTATTATAATATATA

Coding sequence (CDS)

ATGGAGGAAGGTATGGGAGCATTGAAATACTCGCGGACAGCCTATGAAGAGGCATTGGATGTACTGTCCTCGTTAATTGTCAGCCGTAGACGCTGCGATAAATCTGGAGATCGGTTTGAGTTGCTGTTTGAGTATGTAAAGATGCTTGACTTAGAGGAGTCCATGTCGAAAATGAAGGTCATCCATGTAGCTGGCACCAAAGGCAAGGGATCCACGTGCACCTTTACCGAATCCATATTGCGTAAATGTGGCTTCCACACTGGCCTTTTCACTTCTCCTCACCTTATTGATGTCCGAGAAAGATTTCGCTTGGATGGCTTCGACATCAGTGAAGAGAAGTTTTTAGCCTATTTCTGGTGGTGTTATAATAGGCTAAAGGAAAAAACTAGTGAGGATGTACCGATGCCTCACTTGTTTCGCTTCCTAGCGTTACTTGCGTTCAAAATTTTTGCAGATGAGCAGGTGGATGTAGCTATCTTGGAGGTTGGTTTAGGTGGAACTTTTGATGCTACTAATGTGGTCCAGGCACCCATTGTGTGTGGTATAGCTTCTTTGGGTTACGACCACACGGAACTTCTTGGAAACACTCTTGGTGCGATAGCTTCGGAGAAAGCTGGTATCTTCAAGCGTGGGGCTCCAGCTTTTACTGTATGTCAACCTGATGAAGCCATGCATGTGCTTAGAGATAAAGCTTCCCAGTTGGATGTACAACTTACAGTAGTGCCATCGTTGGATCCGGAGCTTCTACAAGGTCATAAACTCGGGCTTGAAGGCGAGCACCAGTATCTAAATGCTCGTCTTGCTATTGCGCTATCCTCCACTTGGCTTCACAGGACCGGCCATTCCAATCTGTCGTACAAGGAACAGAGTGGGTCTTTGCCTGAGCACTTCATAGAAGGGCTTACCACTGCCAGCTTTGAAGGGCGAGCTCAGATTGTTCCTGATAGATATAATGATATTGAATCCCCGGAAGATCTTGTCTTTTATTTGGATGGTGCCCATAGTCCTGAGAGCATGGTGGTTTGCAGTAGCTGGTTTTGTGCTGCAATAAAACATAACCAGGAAGGGATCTTAGATCATCAGTTGGATGATACTGGTACATCTTCAAATAAACTTCTTCAAGGATATGATAATGATGAGGCAAGGAAGAAATCTGTGCAGGTGCTATTGTTCAATTGTATGTCGGTACGAGATCCACAATTGCTTCTTCAACCTCTGATCAAAACGTGCGCAAATAATGGGGTCCACTTCAAGAAGGCAGTCTTTGTTCCAAATACATCATTGTTCAACAAGGTTGGAACCCATGGTTTAGCAGAAGCAAATGGTGAAGTAGACCTTTCTTGGCAGTTCCATGTTCGAAGGGTGTGGGAAAACATCATCCAATCTGAGAAAGGAGGCGATTCTCAAGGCATTGGTGCCATGGTTGAAGAAGAAAGTGAAAAACCAGAAGTGAGTGGTAAGAGCTGCGAGAACAGCGCAGTGTTTTCTTCTCTCCCAATGGCCATTAAATGGCTCCGAGATAGTGTTCTGCAGCAGAACAGGTCCATTCGACTTCAGGTTCTTGTGACTGGTTCTTTACATCTTGTGGGAGATGTGTTGAAATTAATCAAGTGA

Protein sequence

MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKVIHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWWCYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDHQLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK

Homology

[Close]

BLAST of Carg04423 vs. NCBI nr
Match: KAG7033130.1 (Folylpolyglutamate synthase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 537/537 (100.00%), Postives = 537/537 (100.00%), Query Frame = 0


Query: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60
           MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWW 120
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWW 120

Query: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG 300
           VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG 300

Query: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360
           LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH
Sbjct: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360

Query: 361 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420
           QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA
Sbjct: 361 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420

Query: 421 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480
           VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE
Sbjct: 421 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480

Query: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

[Close]

BLAST of Carg04423 vs. NCBI nr
Match: KAG6602447.1 (Folylpolyglutamate synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 536/537 (99.81%), Postives = 536/537 (99.81%), Query Frame = 0


Query: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60
           MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV
Sbjct: 49  MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 108

Query: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWW 120
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDG DISEEKFLAYFWW
Sbjct: 109 IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 168

Query: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 169 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 228

Query: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV
Sbjct: 229 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 288

Query: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG 300
           VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG
Sbjct: 289 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG 348

Query: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360
           LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH
Sbjct: 349 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 408

Query: 361 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420
           QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA
Sbjct: 409 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 468

Query: 421 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480
           VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE
Sbjct: 469 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 528

Query: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 529 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 585

[Close]

BLAST of Carg04423 vs. NCBI nr
Match: XP_022961882.1 (folylpolyglutamate synthase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1073.2 bits (2774), Expect = 8.2e-310
Identity = 534/537 (99.44%), Postives = 534/537 (99.44%), Query Frame = 0


Query: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60
           MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWW 120
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDG DISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 120

Query: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG 300
           VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPE FIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360
           LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH
Sbjct: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360

Query: 361 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420
           QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTC NNGVHFKKA
Sbjct: 361 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCTNNGVHFKKA 420

Query: 421 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480
           VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE
Sbjct: 421 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480

Query: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

[Close]

BLAST of Carg04423 vs. NCBI nr
Match: XP_023533507.1 (folylpolyglutamate synthase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1065.8 bits (2755), Expect = 1.2e-307
Identity = 528/537 (98.32%), Postives = 532/537 (99.07%), Query Frame = 0


Query: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60
           MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFEL+FEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWW 120
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLID+RERFRLDG DISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 120

Query: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIF+DEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG 300
           VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPE FIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360
           LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH
Sbjct: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360

Query: 361 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420
           QLDD GTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA
Sbjct: 361 QLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420

Query: 421 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480
           VFVPNTS+FNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIG MVEEESE
Sbjct: 421 VFVPNTSVFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGTMVEEESE 480

Query: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           KPEVSGK CENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

[Close]

BLAST of Carg04423 vs. NCBI nr
Match: XP_022961890.1 (folylpolyglutamate synthase-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1057.0 bits (2732), Expect = 5.5e-305
Identity = 529/537 (98.51%), Postives = 529/537 (98.51%), Query Frame = 0


Query: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60
           MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWW 120
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDG DISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 120

Query: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG 300
           VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPE FIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360
           LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH
Sbjct: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360

Query: 361 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420
           QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTC NNGVHFKKA
Sbjct: 361 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCTNNGVHFKKA 420

Query: 421 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480
           VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEK     GIGAMVEEESE
Sbjct: 421 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEK-----GIGAMVEEESE 480

Query: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 532

[Close]

BLAST of Carg04423 vs. ExPASy Swiss-Prot
Match: F4K2A1 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS1 PE=1 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 8.9e-181
Identity = 330/528 (62.50%), Postives = 394/528 (74.62%), Query Frame = 0


Query: 14  AYEEALDVLSSLIVSRRRCDKS--GDRFELLFEYVKMLDLEESMSKMKVIHVAGTKGKGS 73
           +YEEAL  LSSLI  R R DKS  GDRFEL+F+Y+K+LDLEE + KM VIHVAGTKGKGS
Sbjct: 66  SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 125

Query: 74  TCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWWCYNRLKEKTSE 133
           TCTFTESI+R  GF TGLFTSPHLIDVRERFRLDG DISEEKFL YFWWCYNRLKE+T+E
Sbjct: 126 TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 185

Query: 134 DVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHTE 193
           ++PMP  FRFLALLAFKIFA E+VD AILEVGLGG FDATN VQ P+VCGI+SLGYDH E
Sbjct: 186 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 245

Query: 194 LLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPELLQG 253
           +LG+TLG IA EKAGIFK G PAFTV QPDEAM VL +KAS+ +V L VV  L   LL G
Sbjct: 246 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSG 305

Query: 254 HKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEGLTTASFEGRAQ 313
            KLGL+GEHQY+NA LA++L+S WL + G   +  + Q   LPE FI+GL TAS +GRAQ
Sbjct: 306 QKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 365

Query: 314 IVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIK-HNQEGILDHQLDDTGTSSN 373
           +VPD+Y +  +  DLVFYLDGAHSPESM  C+ WF  A+K  NQ G   H ++ +  SS+
Sbjct: 366 VVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSH 425

Query: 374 KLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSLFN 433
                + N+       Q+LLFNCMSVRDP LLL  L   CA  GV+FKKA+FVPN S+++
Sbjct: 426 ---DKWSNETCE----QILLFNCMSVRDPNLLLPHLKNMCAKYGVNFKKALFVPNMSVYH 485

Query: 434 KVGTHG-LAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSC 493
           KVGT   L E + +VDLSWQF +++VWE+++QSE+ G                E  G+S 
Sbjct: 486 KVGTAADLPENDPQVDLSWQFTLQKVWESLVQSERDG----------------EKDGESD 545

Query: 494 ENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
            NS VF+SLPMAIK LRD+V + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 546 GNSEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIR 570

[Close]

BLAST of Carg04423 vs. ExPASy Swiss-Prot
Match: Q8W035 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS3 PE=1 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 4.0e-157
Identity = 297/525 (56.57%), Postives = 363/525 (69.14%), Query Frame = 0


Query: 15  YEEALDVLSSLIVSRRR--CDKSGDRFELLFEYVKMLDLEESMSKMKVIHVAGTKGKGST 74
           Y+  LD LSSLI  R R   +    RF LLF Y+K+L+LE+++S+MK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 75  CTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWWCYNRLKEKTSED 134
           CTF ESILR  G  TGLFTSPHLIDVRERFRL+G +IS+EKF+ YFW C+++LKEKTS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 135 VPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHTEL 194
           VPMP  F FLALLAFKIF  EQVDV ILEVGLGG FDATNV+Q P+VCGI+SLGYDH E+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPVVCGISSLGYDHMEI 192

Query: 195 LGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPELLQGH 254
           LG TL  IA+EKAGIFK G PAFTV QPDEAM VL +KAS+L+V L VV  LD       
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 255 KLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEGLTTASFEGRAQI 314
           +LGL+GEHQYLNA LA+AL ST+L   G  + +  +Q+  LPE FI GL+ A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 315 VPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDHQLDDTGTSSNKL 374
           VPD     E PE++V+YLDGAHSPESM  C+ WF   IK NQE                 
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQE----------------- 372

Query: 375 LQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSLFNKV 434
                    +K+S Q+LLFNCMSVRDP LLL  L   C + GV FK+AVFVPN S++N+V
Sbjct: 373 -------RNQKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQV 432

Query: 435 GTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSCENS 494
           G+        E  +SWQF ++R+WE++ + E   +S            K +  GK  E S
Sbjct: 433 GSSTNVGTRVE-SMSWQFGLQRIWESLARGEAKSNS------------KSDSKGKEEEKS 491

Query: 495 AVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
            VFSSLP+A+ WLRD+  +Q++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 FVFSSLPVAVDWLRDNA-RQSKQVRFQVLVTGSLHLVGDLLRFIK 491

[Close]

BLAST of Carg04423 vs. ExPASy Swiss-Prot
Match: F4J2K2 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS2 PE=1 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 3.9e-144
Identity = 279/554 (50.36%), Postives = 369/554 (66.61%), Query Frame = 0


Query: 13  TAYEEALDVLSSLIVSRRRCDK-----SGDRFELLFEYVKMLDLEESMSKMKVIHVAGTK 72
           ++Y++A++ LS+LI  R R D+     + D+ E +  Y+K+LDLE+ + ++KVIHVAGTK
Sbjct: 81  SSYDDAMEALSTLISRRNRGDRTPTKGNRDKLEQVVTYLKILDLEDKIKELKVIHVAGTK 140

Query: 73  GKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWWCYNRLKE 132
           GKGSTC F+E+ILR CGF TG+FTSPHLIDVRERFR+DG DISEEKFL YFW C+  LKE
Sbjct: 141 GKGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRIDGLDISEEKFLQYFWECWKLLKE 200

Query: 133 KTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGY 192
           K  + + MP LF+FL +LAFKIF  E+VDVA++EVGLGG  D+TNV+Q P+VCGIASLG 
Sbjct: 201 KAVDGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVIQKPVVCGIASLGM 260

Query: 193 DHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPE 252
           DH ++LGNTL  IA  KAGIFK   PAFTV Q  EAM VL+  A+ L+V L VV  L+P+
Sbjct: 261 DHMDILGNTLADIAFHKAGIFKPQIPAFTVPQLSEAMDVLQKTANNLEVPLEVVAPLEPK 320

Query: 253 LLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQS--GSLPEHFIEGLTTAS 312
            L G  LGL G+HQ +NA LA++LS  WL RTG+    +  +S    +P  F  GL TA 
Sbjct: 321 KLDGVTLGLSGDHQLVNAGLAVSLSRCWLQRTGNWKKIFPNESKETEIPVAFCRGLATAR 380

Query: 313 FEGRAQIVPDRYND-------IESP-EDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGI 372
             GRAQ+V D  +D       +E+P  DL+FYLDGAHSPESM  C  WF +A++ ++   
Sbjct: 381 LHGRAQVVHDVVSDPQDSSDSMETPCGDLIFYLDGAHSPESMEACGRWFSSAVRGDK--- 440

Query: 373 LDHQLDDTGTSSNKLLQ-GYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVH 432
                    T+ N  ++ G    +  + S Q+LLFNCM VRDPQ+LL  L+ TCA++G H
Sbjct: 441 ------SLSTAVNGYMRHGEYGTDLNRVSKQILLFNCMEVRDPQVLLPKLVTTCASSGTH 500

Query: 433 FKKAVFVPNTSLFNKV--GTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGG-----DSQ 492
           F +A+FVP+ S +NKV  G   +       DL+WQF ++R+WE  IQ    G        
Sbjct: 501 FSRALFVPSMSTYNKVISGASAIPSDTRRKDLTWQFRLQRLWEKSIQGTDAGLDHTLKPD 560

Query: 493 GIGAMVEEE---SEKPEVSGKS---CENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVT 538
           GI A+   +    + P+  G +     +SAV  SLP+ I WLRD V ++N S++L+VLVT
Sbjct: 561 GITALPPHDFLCGDAPQCGGPAGTPVTSSAVMPSLPLTINWLRDCV-RRNPSLKLEVLVT 620

[Close]

BLAST of Carg04423 vs. ExPASy Swiss-Prot
Match: A6H751 (Folylpolyglutamate synthase, mitochondrial OS=Bos taurus OX=9913 GN=FPGS PE=2 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.3e-80
Identity = 213/585 (36.41%), Postives = 296/585 (50.60%), Query Frame = 0


Query: 15  YEEALDVLSSL--------IVSRRRCDKSGDRFELLFEYVKMLDLE-ESMSKMKVIHVAG 74
           Y++A+  L++L        +V R+R D    + E +  Y+    L+ E + ++ +IH+ G
Sbjct: 42  YQDAVRTLNTLQTNANYLEMVKRKRGDPQ-TQLEAMKLYLARSGLQVEDLDQLNIIHITG 101

Query: 75  TKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWWCYNRL 134
           TKGKGSTC FTE ILR  G  TG F+SPHL+ VRER R++G  IS E F  +FW  Y+RL
Sbjct: 102 TKGKGSTCAFTERILRSYGLKTGFFSSPHLVQVRERIRINGQPISSELFTKHFWRLYHRL 161

Query: 135 KEKTSED--VPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIA 194
           +E   +   V MP  FRFL L+AF +F  E+VD+A+LEVG+GG +D TN+++ P+VCGI 
Sbjct: 162 EETKDDSSCVSMPGYFRFLTLMAFHVFLQEKVDLAVLEVGIGGAYDCTNIIRKPVVCGIT 221

Query: 195 SLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPS 254
           SLG DHT LLG+T+  IA +K GIFK G PAFTV QPDE + VLRD+A Q+   L + P 
Sbjct: 222 SLGIDHTGLLGDTMEKIAWQKGGIFKSGVPAFTVLQPDEPLAVLRDRAQQISCPLYLCPP 281

Query: 255 LDPELLQGH---KLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQS-----GSLP- 314
           L   L +G     LGLEGEHQ  NA LA+ ++  WL + G+ +    + S     G LP 
Sbjct: 282 LQ-ALEEGGPPLTLGLEGEHQRSNAALALQVARCWLQQKGYQDAGELKASRPSLPGQLPL 341

Query: 315 -------EHFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFC 374
                      +GL    + GR Q++            L +YLDGAH+  SM  C  WF 
Sbjct: 342 APVFQPTPRMQQGLRHTEWPGRTQLL--------RRGPLTWYLDGAHTTSSMQACVRWFR 401

Query: 375 AAIKHNQEGILDHQLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLI 434
            A+         H+ +   + S                V+VLLFN    RD   LL+ L+
Sbjct: 402 QAL---------HRCERPDSGS---------------EVRVLLFNSTGDRDSAALLK-LL 461

Query: 435 KTCANNGVHFKKAVFVPNTSLFNKVGTHGLAEANGEVD--------LSWQFHVRRVWENI 494
           + C      F  A+F PN  L     T    + N  V         L+ Q H  R+ E  
Sbjct: 462 QPC-----QFDYAIFCPN--LAEMASTDNADQQNFTVTLDQVLLRCLAHQQHWSRLHEEQ 521

Query: 495 IQ----SEKGGDSQGIGAMVEEESEKPEVSGKSCENSAVFSSLPMAIKWL---RDSVLQQ 538
           +     S  G +  G  +++      P        +S VFS +  A++W+   RD + Q 
Sbjct: 522 VSPDPWSTPGQEQDGPASLL----LAPHPPHTHSASSLVFSCISHALQWITQGRDPLFQP 580

[Close]

BLAST of Carg04423 vs. ExPASy Swiss-Prot
Match: P48760 (Folylpolyglutamate synthase, mitochondrial OS=Mus musculus OX=10090 GN=Fpgs PE=1 SV=3)

HSP 1 Score: 300.1 bits (767), Expect = 5.2e-80
Identity = 205/580 (35.34%), Postives = 291/580 (50.17%), Query Frame = 0


Query: 15  YEEALDVLSSL--------IVSRRRCDKSGDRFELLFEYVKMLDLE-ESMSKMKVIHVAG 74
           Y++A+  L++L         V R+R D    + E +  Y+    L+ E ++++ +IHV G
Sbjct: 45  YQDAVRTLNTLQTNASYLEQVKRQRSDPQA-QLEAMEMYLARSGLQVEDLNRLNIIHVTG 104

Query: 75  TKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWWCYNRL 134
           TKGKGSTC FTE ILR  G  TG F+SPH++ VRER R++G  IS E F  +FW  YN+L
Sbjct: 105 TKGKGSTCAFTERILRNYGLKTGFFSSPHMVQVRERIRINGKPISPELFTKHFWCLYNQL 164

Query: 135 KE-KTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIAS 194
           +E K    V MP  FRFL L+AF +F  E+VD+A++EVG+GG FD TN+++ P+VCG++S
Sbjct: 165 EEFKDDSHVSMPSYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAFDCTNIIRKPVVCGVSS 224

Query: 195 LGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSL 254
           LG DHT LLG+T+  IA +K GIFK G PAFTV QP+  + VLRD+A Q+   L + P L
Sbjct: 225 LGIDHTSLLGDTVEKIAWQKGGIFKPGVPAFTVVQPEGPLAVLRDRAQQIGCPLYLCPPL 284

Query: 255 D--PELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQS-----GSLP--- 314
           +   E+     LGLEG HQ  NA LA+ L+  WL R  H ++   + S       LP   
Sbjct: 285 EALEEVGLPLSLGLEGAHQRSNAALALQLAHCWLERQDHQDIQELKVSRPSIRWQLPLAP 344

Query: 315 -----EHFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAA 374
                 H   GL    + GR QI+            L +YLDGAH+  S+  C  W+  +
Sbjct: 345 VFRPTPHMRRGLRDTVWPGRTQIL--------QRGPLTWYLDGAHTTSSVQACVHWYRQS 404

Query: 375 IKHNQEGILDHQLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKT 434
           ++ ++         D G+                  V +LLFN    RD   LL+ L++ 
Sbjct: 405 LERSKR-------TDGGS-----------------EVHILLFNSTGDRDSAALLK-LLQP 464

Query: 435 CANNGVHFKKAVFVPNTSLFNKVGTHGLAEANGEVD----------LSWQFHVRRVWENI 494
           C      F  AVF PN +  + +G          +D            W     +   + 
Sbjct: 465 C-----QFDYAVFCPNVTEVSSIGNADQQNFTVTLDQVLLRCLQHQQHWNGLAEKQASSN 524

Query: 495 IQSEKGGDSQGIGAMVEEESEKPEVSGKSCENSAVFSSLPMAIKWL---RDSVLQQ---- 537
           + S  G D  G G+++      P     +  +S VFS +  A+ W+   RD + Q     
Sbjct: 525 LWSSCGPDPAGPGSLL----LAPHPPQPTRTSSLVFSCISHALLWISQGRDPIFQPQSLP 581

[Close]

BLAST of Carg04423 vs. ExPASy TrEMBL
Match: A0A6J1HBB1 (Folylpolyglutamate synthase OS=Cucurbita moschata OX=3662 GN=LOC111462519 PE=3 SV=1)

HSP 1 Score: 1073.2 bits (2774), Expect = 4.0e-310
Identity = 534/537 (99.44%), Postives = 534/537 (99.44%), Query Frame = 0


Query: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60
           MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWW 120
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDG DISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 120

Query: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG 300
           VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPE FIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360
           LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH
Sbjct: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360

Query: 361 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420
           QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTC NNGVHFKKA
Sbjct: 361 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCTNNGVHFKKA 420

Query: 421 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480
           VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE
Sbjct: 421 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480

Query: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

[Close]

BLAST of Carg04423 vs. ExPASy TrEMBL
Match: A0A6J1HDI1 (Folylpolyglutamate synthase OS=Cucurbita moschata OX=3662 GN=LOC111462519 PE=3 SV=1)

HSP 1 Score: 1057.0 bits (2732), Expect = 2.7e-305
Identity = 529/537 (98.51%), Postives = 529/537 (98.51%), Query Frame = 0


Query: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60
           MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWW 120
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDG DISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 120

Query: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG 300
           VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPE FIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360
           LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH
Sbjct: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360

Query: 361 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420
           QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTC NNGVHFKKA
Sbjct: 361 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCTNNGVHFKKA 420

Query: 421 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480
           VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEK     GIGAMVEEESE
Sbjct: 421 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEK-----GIGAMVEEESE 480

Query: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 532

[Close]

BLAST of Carg04423 vs. ExPASy TrEMBL
Match: A0A6J1JQH1 (Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=1)

HSP 1 Score: 1053.5 bits (2723), Expect = 2.9e-304
Identity = 523/537 (97.39%), Postives = 530/537 (98.70%), Query Frame = 0


Query: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60
           MEEGMGALKYSRTAYEEALDVL SLIV+RRRCDKSGDRFEL+FEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLFSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWW 120
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLID+RERFRLDG DISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 120

Query: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIF+DEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTV QPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG 300
           VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTG SNLSYKEQSGSLPE FIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGRSNLSYKEQSGSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360
           LTTA FEGRAQIVPDRYNDIE+PEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGIL+H
Sbjct: 301 LTTARFEGRAQIVPDRYNDIETPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILNH 360

Query: 361 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420
           QLDD GTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA
Sbjct: 361 QLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420

Query: 421 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480
           +FVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE
Sbjct: 421 LFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480

Query: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

[Close]

BLAST of Carg04423 vs. ExPASy TrEMBL
Match: A0A6J1JTP4 (Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=1)

HSP 1 Score: 1038.5 bits (2684), Expect = 9.8e-300
Identity = 523/565 (92.57%), Postives = 530/565 (93.81%), Query Frame = 0


Query: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60
           MEEGMGALKYSRTAYEEALDVL SLIV+RRRCDKSGDRFEL+FEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLFSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWW 120
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLID+RERFRLDG DISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 120

Query: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIF+DEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHTELLGNTLGAIASEKAGIFK----------------------------RGA 240
           GIASLGYDHTELLGNTLGAIASEKAGIFK                            RGA
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKVCLKKLSLKHERLGIISSFCFHPFTLVQRGA 240

Query: 241 PAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALS 300
           PAFTV QPDEAMHVLRDKASQLDVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALS
Sbjct: 241 PAFTVFQPDEAMHVLRDKASQLDVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALS 300

Query: 301 STWLHRTGHSNLSYKEQSGSLPEHFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDG 360
           STWLHRTG SNLSYKEQSGSLPE FIEGLTTA FEGRAQIVPDRYNDIE+PEDLVFYLDG
Sbjct: 301 STWLHRTGRSNLSYKEQSGSLPEQFIEGLTTARFEGRAQIVPDRYNDIETPEDLVFYLDG 360

Query: 361 AHSPESMVVCSSWFCAAIKHNQEGILDHQLDDTGTSSNKLLQGYDNDEARKKSVQVLLFN 420
           AHSPESMVVCSSWFCAAIKHNQEGIL+HQLDD GTSSNKLLQGYDNDEARKKSVQVLLFN
Sbjct: 361 AHSPESMVVCSSWFCAAIKHNQEGILNHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFN 420

Query: 421 CMSVRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSLFNKVGTHGLAEANGEVDLSWQFHV 480
           CMSVRDPQLLLQPLIKTCANNGVHFKKA+FVPNTSLFNKVGTHGLAEANGEVDLSWQFHV
Sbjct: 421 CMSVRDPQLLLQPLIKTCANNGVHFKKALFVPNTSLFNKVGTHGLAEANGEVDLSWQFHV 480

Query: 481 RRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQ 538
           RRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQ
Sbjct: 481 RRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQ 540

[Close]

BLAST of Carg04423 vs. ExPASy TrEMBL
Match: A0A6J1JNC2 (Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=1)

HSP 1 Score: 1037.3 bits (2681), Expect = 2.2e-299
Identity = 518/537 (96.46%), Postives = 525/537 (97.77%), Query Frame = 0


Query: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60
           MEEGMGALKYSRTAYEEALDVL SLIV+RRRCDKSGDRFEL+FEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLFSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWW 120
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLID+RERFRLDG DISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 120

Query: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIF+DEQVDVAILEVGLGGTFDATNVVQAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTV QPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEG 300
           VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTG SNLSYKEQSGSLPE FIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGRSNLSYKEQSGSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360
           LTTA FEGRAQIVPDRYNDIE+PEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGIL+H
Sbjct: 301 LTTARFEGRAQIVPDRYNDIETPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILNH 360

Query: 361 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420
           QLDD GTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA
Sbjct: 361 QLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420

Query: 421 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480
           +FVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEK     GIGAMVEEESE
Sbjct: 421 LFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEK-----GIGAMVEEESE 480

Query: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 532

[Close]

BLAST of Carg04423 vs. TAIR 10
Match: AT5G05980.1 (DHFS-FPGS homolog B )

HSP 1 Score: 634.8 bits (1636), Expect = 6.3e-182
Identity = 330/528 (62.50%), Postives = 394/528 (74.62%), Query Frame = 0


Query: 14  AYEEALDVLSSLIVSRRRCDKS--GDRFELLFEYVKMLDLEESMSKMKVIHVAGTKGKGS 73
           +YEEAL  LSSLI  R R DKS  GDRFEL+F+Y+K+LDLEE + KM VIHVAGTKGKGS
Sbjct: 66  SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 125

Query: 74  TCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWWCYNRLKEKTSE 133
           TCTFTESI+R  GF TGLFTSPHLIDVRERFRLDG DISEEKFL YFWWCYNRLKE+T+E
Sbjct: 126 TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 185

Query: 134 DVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHTE 193
           ++PMP  FRFLALLAFKIFA E+VD AILEVGLGG FDATN VQ P+VCGI+SLGYDH E
Sbjct: 186 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 245

Query: 194 LLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPELLQG 253
           +LG+TLG IA EKAGIFK G PAFTV QPDEAM VL +KAS+ +V L VV  L   LL G
Sbjct: 246 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSG 305

Query: 254 HKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEGLTTASFEGRAQ 313
            KLGL+GEHQY+NA LA++L+S WL + G   +  + Q   LPE FI+GL TAS +GRAQ
Sbjct: 306 QKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 365

Query: 314 IVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIK-HNQEGILDHQLDDTGTSSN 373
           +VPD+Y +  +  DLVFYLDGAHSPESM  C+ WF  A+K  NQ G   H ++ +  SS+
Sbjct: 366 VVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSH 425

Query: 374 KLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSLFN 433
                + N+       Q+LLFNCMSVRDP LLL  L   CA  GV+FKKA+FVPN S+++
Sbjct: 426 ---DKWSNETCE----QILLFNCMSVRDPNLLLPHLKNMCAKYGVNFKKALFVPNMSVYH 485

Query: 434 KVGTHG-LAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSC 493
           KVGT   L E + +VDLSWQF +++VWE+++QSE+ G                E  G+S 
Sbjct: 486 KVGTAADLPENDPQVDLSWQFTLQKVWESLVQSERDG----------------EKDGESD 545

Query: 494 ENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
            NS VF+SLPMAIK LRD+V + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 546 GNSEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIR 570

[Close]

BLAST of Carg04423 vs. TAIR 10
Match: AT5G05980.2 (DHFS-FPGS homolog B )

HSP 1 Score: 634.8 bits (1636), Expect = 6.3e-182
Identity = 330/528 (62.50%), Postives = 394/528 (74.62%), Query Frame = 0


Query: 14  AYEEALDVLSSLIVSRRRCDKS--GDRFELLFEYVKMLDLEESMSKMKVIHVAGTKGKGS 73
           +YEEAL  LSSLI  R R DKS  GDRFEL+F+Y+K+LDLEE + KM VIHVAGTKGKGS
Sbjct: 8   SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 67

Query: 74  TCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWWCYNRLKEKTSE 133
           TCTFTESI+R  GF TGLFTSPHLIDVRERFRLDG DISEEKFL YFWWCYNRLKE+T+E
Sbjct: 68  TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 127

Query: 134 DVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHTE 193
           ++PMP  FRFLALLAFKIFA E+VD AILEVGLGG FDATN VQ P+VCGI+SLGYDH E
Sbjct: 128 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 187

Query: 194 LLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPELLQG 253
           +LG+TLG IA EKAGIFK G PAFTV QPDEAM VL +KAS+ +V L VV  L   LL G
Sbjct: 188 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSG 247

Query: 254 HKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEGLTTASFEGRAQ 313
            KLGL+GEHQY+NA LA++L+S WL + G   +  + Q   LPE FI+GL TAS +GRAQ
Sbjct: 248 QKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 307

Query: 314 IVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIK-HNQEGILDHQLDDTGTSSN 373
           +VPD+Y +  +  DLVFYLDGAHSPESM  C+ WF  A+K  NQ G   H ++ +  SS+
Sbjct: 308 VVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSH 367

Query: 374 KLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSLFN 433
                + N+       Q+LLFNCMSVRDP LLL  L   CA  GV+FKKA+FVPN S+++
Sbjct: 368 ---DKWSNETCE----QILLFNCMSVRDPNLLLPHLKNMCAKYGVNFKKALFVPNMSVYH 427

Query: 434 KVGTHG-LAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSC 493
           KVGT   L E + +VDLSWQF +++VWE+++QSE+ G                E  G+S 
Sbjct: 428 KVGTAADLPENDPQVDLSWQFTLQKVWESLVQSERDG----------------EKDGESD 487

Query: 494 ENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
            NS VF+SLPMAIK LRD+V + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 488 GNSEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIR 512

[Close]

BLAST of Carg04423 vs. TAIR 10
Match: AT3G55630.3 (DHFS-FPGS homolog D )

HSP 1 Score: 556.2 bits (1432), Expect = 2.8e-158
Identity = 297/525 (56.57%), Postives = 363/525 (69.14%), Query Frame = 0


Query: 15  YEEALDVLSSLIVSRRR--CDKSGDRFELLFEYVKMLDLEESMSKMKVIHVAGTKGKGST 74
           Y+  LD LSSLI  R R   +    RF LLF Y+K+L+LE+++S+MK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 75  CTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWWCYNRLKEKTSED 134
           CTF ESILR  G  TGLFTSPHLIDVRERFRL+G +IS+EKF+ YFW C+++LKEKTS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 135 VPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHTEL 194
           VPMP  F FLALLAFKIF  EQVDV ILEVGLGG FDATNV+Q P+VCGI+SLGYDH E+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPVVCGISSLGYDHMEI 192

Query: 195 LGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPELLQGH 254
           LG TL  IA+EKAGIFK G PAFTV QPDEAM VL +KAS+L+V L VV  LD       
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 255 KLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEGLTTASFEGRAQI 314
           +LGL+GEHQYLNA LA+AL ST+L   G  + +  +Q+  LPE FI GL+ A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 315 VPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDHQLDDTGTSSNKL 374
           VPD     E PE++V+YLDGAHSPESM  C+ WF   IK NQE                 
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQE----------------- 372

Query: 375 LQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSLFNKV 434
                    +K+S Q+LLFNCMSVRDP LLL  L   C + GV FK+AVFVPN S++N+V
Sbjct: 373 -------RNQKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQV 432

Query: 435 GTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSCENS 494
           G+        E  +SWQF ++R+WE++ + E   +S            K +  GK  E S
Sbjct: 433 GSSTNVGTRVE-SMSWQFGLQRIWESLARGEAKSNS------------KSDSKGKEEEKS 491

Query: 495 AVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
            VFSSLP+A+ WLRD+  +Q++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 FVFSSLPVAVDWLRDNA-RQSKQVRFQVLVTGSLHLVGDLLRFIK 491

[Close]

BLAST of Carg04423 vs. TAIR 10
Match: AT3G55630.2 (DHFS-FPGS homolog D )

HSP 1 Score: 550.1 bits (1416), Expect = 2.0e-156
Identity = 296/525 (56.38%), Postives = 362/525 (68.95%), Query Frame = 0


Query: 15  YEEALDVLSSLIVSRRR--CDKSGDRFELLFEYVKMLDLEESMSKMKVIHVAGTKGKGST 74
           Y+  LD LSSLI  R R   +    RF LLF Y+K+L+LE+++S+MK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 75  CTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWWCYNRLKEKTSED 134
           CTF ESILR  G  TGLFTSPHLIDVRERFRL+G +IS+EKF+ YFW C+++LKEKTS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 135 VPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHTEL 194
           VPMP  F FLALLAFKIF  EQVDV ILEVGLGG FDATN +Q P+VCGI+SLGYDH E+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATN-IQKPVVCGISSLGYDHMEI 192

Query: 195 LGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPELLQGH 254
           LG TL  IA+EKAGIFK G PAFTV QPDEAM VL +KAS+L+V L VV  LD       
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 255 KLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEHFIEGLTTASFEGRAQI 314
           +LGL+GEHQYLNA LA+AL ST+L   G  + +  +Q+  LPE FI GL+ A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 315 VPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDHQLDDTGTSSNKL 374
           VPD     E PE++V+YLDGAHSPESM  C+ WF   IK NQE                 
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQE----------------- 372

Query: 375 LQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSLFNKV 434
                    +K+S Q+LLFNCMSVRDP LLL  L   C + GV FK+AVFVPN S++N+V
Sbjct: 373 -------RNQKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQV 432

Query: 435 GTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSCENS 494
           G+        E  +SWQF ++R+WE++ + E   +S            K +  GK  E S
Sbjct: 433 GSSTNVGTRVE-SMSWQFGLQRIWESLARGEAKSNS------------KSDSKGKEEEKS 490

Query: 495 AVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
            VFSSLP+A+ WLRD+  +Q++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 FVFSSLPVAVDWLRDNA-RQSKQVRFQVLVTGSLHLVGDLLRFIK 490

[Close]

BLAST of Carg04423 vs. TAIR 10
Match: AT3G10160.1 (DHFS-FPGS homolog C )

HSP 1 Score: 513.1 bits (1320), Expect = 2.8e-145
Identity = 279/554 (50.36%), Postives = 369/554 (66.61%), Query Frame = 0


Query: 13  TAYEEALDVLSSLIVSRRRCDK-----SGDRFELLFEYVKMLDLEESMSKMKVIHVAGTK 72
           ++Y++A++ LS+LI  R R D+     + D+ E +  Y+K+LDLE+ + ++KVIHVAGTK
Sbjct: 81  SSYDDAMEALSTLISRRNRGDRTPTKGNRDKLEQVVTYLKILDLEDKIKELKVIHVAGTK 140

Query: 73  GKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWWCYNRLKE 132
           GKGSTC F+E+ILR CGF TG+FTSPHLIDVRERFR+DG DISEEKFL YFW C+  LKE
Sbjct: 141 GKGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRIDGLDISEEKFLQYFWECWKLLKE 200

Query: 133 KTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGY 192
           K  + + MP LF+FL +LAFKIF  E+VDVA++EVGLGG  D+TNV+Q P+VCGIASLG 
Sbjct: 201 KAVDGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVIQKPVVCGIASLGM 260

Query: 193 DHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVPSLDPE 252
           DH ++LGNTL  IA  KAGIFK   PAFTV Q  EAM VL+  A+ L+V L VV  L+P+
Sbjct: 261 DHMDILGNTLADIAFHKAGIFKPQIPAFTVPQLSEAMDVLQKTANNLEVPLEVVAPLEPK 320

Query: 253 LLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQS--GSLPEHFIEGLTTAS 312
            L G  LGL G+HQ +NA LA++LS  WL RTG+    +  +S    +P  F  GL TA 
Sbjct: 321 KLDGVTLGLSGDHQLVNAGLAVSLSRCWLQRTGNWKKIFPNESKETEIPVAFCRGLATAR 380

Query: 313 FEGRAQIVPDRYND-------IESP-EDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGI 372
             GRAQ+V D  +D       +E+P  DL+FYLDGAHSPESM  C  WF +A++ ++   
Sbjct: 381 LHGRAQVVHDVVSDPQDSSDSMETPCGDLIFYLDGAHSPESMEACGRWFSSAVRGDK--- 440

Query: 373 LDHQLDDTGTSSNKLLQ-GYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVH 432
                    T+ N  ++ G    +  + S Q+LLFNCM VRDPQ+LL  L+ TCA++G H
Sbjct: 441 ------SLSTAVNGYMRHGEYGTDLNRVSKQILLFNCMEVRDPQVLLPKLVTTCASSGTH 500

Query: 433 FKKAVFVPNTSLFNKV--GTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGG-----DSQ 492
           F +A+FVP+ S +NKV  G   +       DL+WQF ++R+WE  IQ    G        
Sbjct: 501 FSRALFVPSMSTYNKVISGASAIPSDTRRKDLTWQFRLQRLWEKSIQGTDAGLDHTLKPD 560

Query: 493 GIGAMVEEE---SEKPEVSGKS---CENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVT 538
           GI A+   +    + P+  G +     +SAV  SLP+ I WLRD V ++N S++L+VLVT
Sbjct: 561 GITALPPHDFLCGDAPQCGGPAGTPVTSSAVMPSLPLTINWLRDCV-RRNPSLKLEVLVT 620

The following BLAST results are available for this feature:

BLAST of Carg04423 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7033130.1	0.0e+00	100.00	Folylpolyglutamate synthase [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6602447.1	0.0e+00	99.81	Folylpolyglutamate synthase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022961882.1	8.2e-310	99.44	folylpolyglutamate synthase-like isoform X1 [Cucurbita moschata]	[more]
XP_023533507.1	1.2e-307	98.32	folylpolyglutamate synthase-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022961890.1	5.5e-305	98.51	folylpolyglutamate synthase-like isoform X2 [Cucurbita moschata]	[more]

BLAST of Carg04423 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4K2A1	8.9e-181	62.50	Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS1 PE=1 SV=1	[more]
Q8W035	4.0e-157	56.57	Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS3 PE=1 SV=1	[more]
F4J2K2	3.9e-144	50.36	Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS2 PE=1 SV=1	[more]
A6H751	2.3e-80	36.41	Folylpolyglutamate synthase, mitochondrial OS=Bos taurus OX=9913 GN=FPGS PE=2 SV...	[more]
P48760	5.2e-80	35.34	Folylpolyglutamate synthase, mitochondrial OS=Mus musculus OX=10090 GN=Fpgs PE=1...	[more]

BLAST of Carg04423 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HBB1	4.0e-310	99.44	Folylpolyglutamate synthase OS=Cucurbita moschata OX=3662 GN=LOC111462519 PE=3 S...	[more]
A0A6J1HDI1	2.7e-305	98.51	Folylpolyglutamate synthase OS=Cucurbita moschata OX=3662 GN=LOC111462519 PE=3 S...	[more]
A0A6J1JQH1	2.9e-304	97.39	Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=...	[more]
A0A6J1JTP4	9.8e-300	92.57	Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=...	[more]
A0A6J1JNC2	2.2e-299	96.46	Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=...	[more]

BLAST of Carg04423 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G05980.1	6.3e-182	62.50	DHFS-FPGS homolog B	[more]
AT5G05980.2	6.3e-182	62.50	DHFS-FPGS homolog B	[more]
AT3G55630.3	2.8e-158	56.57	DHFS-FPGS homolog D	[more]
AT3G55630.2	2.0e-156	56.38	DHFS-FPGS homolog D	[more]
AT3G10160.1	2.8e-145	50.36	DHFS-FPGS homolog C	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036565	Mur-like, catalytic domain superfamily	GENE3D	3.40.1190.10		coord: 6..289 e-value: 8.9E-89 score: 299.4
IPR036565	Mur-like, catalytic domain superfamily	SUPERFAMILY	53623	MurD-like peptide ligases, catalytic domain	coord: 31..277
IPR036615	Mur ligase, C-terminal domain superfamily	GENE3D	3.90.190.20		coord: 308..537 e-value: 1.1E-9 score: 40.7
IPR036615	Mur ligase, C-terminal domain superfamily	SUPERFAMILY	53244	MurD-like peptide ligases, peptide-binding domain	coord: 308..536
IPR023600	Folylpolyglutamate synthase, eukaryota	PIRSF	PIRSF038895	FPGS	coord: 3..537 e-value: 1.5E-149 score: 496.6
IPR013221	Mur ligase, central	PFAM	PF08245	Mur_ligase_M	coord: 63..235 e-value: 1.6E-4 score: 21.7
IPR001645	Folylpolyglutamate synthetase	TIGRFAM	TIGR01499	TIGR01499	coord: 52..352 e-value: 1.1E-86 score: 289.6
IPR001645	Folylpolyglutamate synthetase	PANTHER	PTHR11136	FOLYLPOLYGLUTAMATE SYNTHASE-RELATED	coord: 11..537
None	No IPR available	PANTHER	PTHR11136:SF10	FOLYLPOLYGLUTAMATE SYNTHASE	coord: 11..537
IPR018109	Folylpolyglutamate synthetase, conserved site	PROSITE	PS01011	FOLYLPOLYGLU_SYNT_1	coord: 61..84
IPR018109	Folylpolyglutamate synthetase, conserved site	PROSITE	PS01012	FOLYLPOLYGLU_SYNT_2	coord: 159..174

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg04423-RA	Carg04423-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006730	one-carbon metabolic process
biological_process	GO:0046901	tetrahydrofolylpolyglutamate biosynthetic process
biological_process	GO:0009058	biosynthetic process
biological_process	GO:0009396	folic acid-containing compound biosynthetic process
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004326	tetrahydrofolylpolyglutamate synthase activity
molecular_function	GO:0016874	ligase activity