Carg04413 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04413
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	ZnMc domain-containing protein
Location	Carg_Chr04: 20513954 .. 20514642 (+)
RNA-Seq Expression	Carg04413
Synteny	Carg04413

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTAATGAGCACCAAAAAATGGCAAAAAGTTCCATTGTTTATGGCTTCCAGACACTTGGTTCTTTAGGCTGTGACCCAGCCAAGGGTATACCATATTGCCTGTATGCGTCGTGGTGGGTCGAGGGTCATTTTCCGCAACCCCTGGTCCAGTGCTCTACCTTCATCAAACTGACAATATCAAAAATAAAATTTCGAGAATTTTTGTTTAAAATGATATCAGAGTCATGTCTTTATCTTTACTTAATCATGTCAATAATGACTCAGGTGTCGGATTCGGATGGTATGATCATTTTTTCCCCTATTTAAGCATATCAATAGACGTGTGGCTAATATCTTTCATAGCAATGGCCTTCATAGCTCTCCAATTCTTCTCCTTCATCCTTCTACTCTCCTTCTTCCCTCATTTAAACATCTCATCTCCGTTGATTTTGAATCATAATTCCCACGGCATGAATTCATCTTTCATGTTGTTTCTAAAAGTACCTTCAACGCTTTGGTTACATTACCAATGTCCAAAAACACTCCAACGCCATGGATCATGATGACACCTTTGATAAAGCAGCTGATATGAACATAAGCTTTGAAAAACAAGAGCATGGAGATATTGATCCGTTTAATGGTGCGGGAAAAATTTTGGCCCATGCTTTTCCACCCACGGATTGTACTCCTTCTGGGGCTTCAACATAG

mRNA sequence

ATGTACCTTCAACGCTTTGGTTACATTACCAATGTCCAAAAACACTCCAACGCCATGGATCATGATGACACCTTTGATAAAGCAGCTGATATGAACATAAGCTTTGAAAAACAAGAGCATGGAGATATTGATCCGTTTAATGGTGCGGGAAAAATTTTGGCCCATGCTTTTCCACCCACGGATTGTACTCCTTCTGGGGCTTCAACATAG

Coding sequence (CDS)

ATGTACCTTCAACGCTTTGGTTACATTACCAATGTCCAAAAACACTCCAACGCCATGGATCATGATGACACCTTTGATAAAGCAGCTGATATGAACATAAGCTTTGAAAAACAAGAGCATGGAGATATTGATCCGTTTAATGGTGCGGGAAAAATTTTGGCCCATGCTTTTCCACCCACGGATTGTACTCCTTCTGGGGCTTCAACATAG

Protein sequence

MYLQRFGYITNVQKHSNAMDHDDTFDKAADMNISFEKQEHGDIDPFNGAGKILAHAFPPTDCTPSGAST

Homology

[Close]

BLAST of Carg04413 vs. NCBI nr
Match: KAG7033140.1 (Stromelysin-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 148.3 bits (373), Expect = 2.5e-32
Identity = 69/69 (100.00%), Postives = 69/69 (100.00%), Query Frame = 0


Query: 1  MYLQRFGYITNVQKHSNAMDHDDTFDKAADMNISFEKQEHGDIDPFNGAGKILAHAFPPT 60
          MYLQRFGYITNVQKHSNAMDHDDTFDKAADMNISFEKQEHGDIDPFNGAGKILAHAFPPT
Sbjct: 1  MYLQRFGYITNVQKHSNAMDHDDTFDKAADMNISFEKQEHGDIDPFNGAGKILAHAFPPT 60

Query: 61 DCTPSGAST 70
          DCTPSGAST
Sbjct: 61 DCTPSGAST 69

[Close]

BLAST of Carg04413 vs. NCBI nr
Match: XP_023552591.1 (metalloendoproteinase 5-MMP-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 59.3 bits (142), Expect = 1.5e-05
Identity = 26/29 (89.66%), Postives = 27/29 (93.10%), Query Frame = 0


Query: 2   YLQRFGYITNVQKHSNAMDHDDTFDKAAD 31
           YLQRFGYITNVQKHSNAMDHDDTFD A +
Sbjct: 78  YLQRFGYITNVQKHSNAMDHDDTFDNALE 106

[Close]

BLAST of Carg04413 vs. NCBI nr
Match: KAG6602462.1 (Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 56.2 bits (134), Expect = 1.3e-04
Identity = 25/29 (86.21%), Postives = 26/29 (89.66%), Query Frame = 0


Query: 2   YLQRFGYITNVQKHSNAMDHDDTFDKAAD 31
           YLQRFGYITNVQK SNAMDHDDTFD A +
Sbjct: 79  YLQRFGYITNVQKQSNAMDHDDTFDNALE 107

[Close]

BLAST of Carg04413 vs. NCBI nr
Match: XP_022962668.1 (metalloendoproteinase 2-MMP-like [Cucurbita moschata])

HSP 1 Score: 56.2 bits (134), Expect = 1.3e-04
Identity = 25/29 (86.21%), Postives = 26/29 (89.66%), Query Frame = 0


Query: 2   YLQRFGYITNVQKHSNAMDHDDTFDKAAD 31
           YLQRFGYITNVQK SNAMDHDDTFD A +
Sbjct: 79  YLQRFGYITNVQKQSNAMDHDDTFDNALE 107

[Close]

BLAST of Carg04413 vs. NCBI nr
Match: XP_022991021.1 (metalloendoproteinase 2-MMP-like [Cucurbita maxima])

HSP 1 Score: 56.2 bits (134), Expect = 1.3e-04
Identity = 24/29 (82.76%), Postives = 26/29 (89.66%), Query Frame = 0


Query: 2   YLQRFGYITNVQKHSNAMDHDDTFDKAAD 31
           YLQRFGYI+NVQKHSNAMDHDDTFD   +
Sbjct: 79  YLQRFGYISNVQKHSNAMDHDDTFDNTLE 107

[Close]

BLAST of Carg04413 vs. ExPASy Swiss-Prot
Match: Q6Y4Q5 (Stromelysin-1 OS=Canis lupus familiaris OX=9615 GN=MMP3 PE=2 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 3.5e-05
Identity = 21/49 (42.86%), Postives = 30/49 (61.22%), Query Frame = 0


Query: 11  NVQKHSNAMDHDDTFDKAADMNISFEKQEHGDIDPFNGAGKILAHAFPP 60
           NV K    +    T +  AD+ ISF  ++HGD +PF+G G +L HA+PP
Sbjct: 139 NVWKEVTPLTFSRTDEGEADIKISFAVRDHGDFNPFDGPGNVLGHAYPP 187

[Close]

BLAST of Carg04413 vs. ExPASy Swiss-Prot
Match: Q28397 (Stromelysin-1 OS=Equus caballus OX=9796 GN=MMP3 PE=2 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 5.9e-05
Identity = 19/35 (54.29%), Postives = 27/35 (77.14%), Query Frame = 0


Query: 25  FDKAADMNISFEKQEHGDIDPFNGAGKILAHAFPP 60
           ++  AD+ I+F  +EHGD  PF+G GK+LAHA+PP
Sbjct: 153 YEGEADIMITFAVREHGDFFPFDGPGKVLAHAYPP 187

[Close]

BLAST of Carg04413 vs. ExPASy Swiss-Prot
Match: P33435 (Collagenase 3 OS=Mus musculus OX=10090 GN=Mmp13 PE=1 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 1.3e-04
Identity = 20/35 (57.14%), Postives = 25/35 (71.43%), Query Frame = 0


Query: 25  FDKAADMNISFEKQEHGDIDPFNGAGKILAHAFPP 60
           +D  AD+ ISF  +EHGD  PF+G   +LAHAFPP
Sbjct: 158 YDGTADIMISFGTKEHGDFYPFDGPSGLLAHAFPP 192

[Close]

BLAST of Carg04413 vs. ExPASy Swiss-Prot
Match: P23097 (Collagenase 3 (Fragment) OS=Rattus norvegicus OX=10116 GN=Mmp13 PE=1 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 2.9e-04
Identity = 20/34 (58.82%), Postives = 24/34 (70.59%), Query Frame = 0


Query: 26  DKAADMNISFEKQEHGDIDPFNGAGKILAHAFPP 60
           D  AD+ ISF  +EHGD  PF+G   +LAHAFPP
Sbjct: 153 DGTADIMISFGTKEHGDFYPFDGPSGLLAHAFPP 186

[Close]

BLAST of Carg04413 vs. ExPASy Swiss-Prot
Match: Q10835 (Collagenase 3 (Fragment) OS=Xenopus laevis OX=8355 GN=mmp13 PE=2 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 3.9e-04
Identity = 19/31 (61.29%), Postives = 24/31 (77.42%), Query Frame = 0


Query: 29  ADMNISFEKQEHGDIDPFNGAGKILAHAFPP 60
           AD+ +SF K+EHGD  PF+G   +LAHAFPP
Sbjct: 159 ADIMVSFGKKEHGDYYPFDGPDGLLAHAFPP 189

[Close]

BLAST of Carg04413 vs. ExPASy TrEMBL
Match: A0A6J1JPK1 (metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111487736 PE=3 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 6.2e-05
Identity = 24/29 (82.76%), Postives = 26/29 (89.66%), Query Frame = 0


Query: 2   YLQRFGYITNVQKHSNAMDHDDTFDKAAD 31
           YLQRFGYI+NVQKHSNAMDHDDTFD   +
Sbjct: 79  YLQRFGYISNVQKHSNAMDHDDTFDNTLE 107

[Close]

BLAST of Carg04413 vs. ExPASy TrEMBL
Match: A0A6J1HFG1 (metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111463086 PE=3 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 6.2e-05
Identity = 25/29 (86.21%), Postives = 26/29 (89.66%), Query Frame = 0


Query: 2   YLQRFGYITNVQKHSNAMDHDDTFDKAAD 31
           YLQRFGYITNVQK SNAMDHDDTFD A +
Sbjct: 79  YLQRFGYITNVQKQSNAMDHDDTFDNALE 107

[Close]

BLAST of Carg04413 vs. ExPASy TrEMBL
Match: A0A6S7PL12 (ZnMc domain-containing protein OS=Lactuca saligna OX=75948 GN=LSAL_LOCUS42559 PE=3 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.1e-04
Identity = 28/53 (52.83%), Postives = 36/53 (67.92%), Query Frame = 0


Query: 14  KHSNAMDHDDTFDKA-----ADMNISFEKQEHGDIDPFNGAGKILAHAFPPTD 62
           K + A     TF +A     AD+ ISFE+ +HGD  PFNG+G++LAHAFPPTD
Sbjct: 166 KWATASSRYFTFSRAGSYQGADLKISFERGDHGDGYPFNGSGEVLAHAFPPTD 218

[Close]

BLAST of Carg04413 vs. ExPASy TrEMBL
Match: A0A6J1BXD5 (metalloendoproteinase 2-MMP-like OS=Momordica charantia OX=3673 GN=LOC111006498 PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 3.1e-04
Identity = 27/46 (58.70%), Postives = 32/46 (69.57%), Query Frame = 0


Query: 16  SNAMDHDDTFDKAADMNISFEKQEHGDIDPFNGAGKILAHAFPPTD 62
           S+  DH     K AD+ ISFE +EHGD   F+GAG ILAHA+PPTD
Sbjct: 209 SHVSDH-----KTADIKISFEIEEHGDDAAFDGAGGILAHAYPPTD 249

[Close]

BLAST of Carg04413 vs. ExPASy TrEMBL
Match: A0A6S7NKS7 (ZnMc domain-containing protein OS=Lactuca saligna OX=75948 GN=LSAL_LOCUS24722 PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 3.1e-04
Identity = 22/35 (62.86%), Postives = 29/35 (82.86%), Query Frame = 0


Query: 27  KAADMNISFEKQEHGDIDPFNGAGKILAHAFPPTD 62
           + AD+ ISFE+ +HGD  PFNG+G++LAHAFPP D
Sbjct: 223 EGADLKISFERGDHGDGYPFNGSGEVLAHAFPPPD 257

The following BLAST results are available for this feature:

BLAST of Carg04413 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7033140.1	2.5e-32	100.00	Stromelysin-1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023552591.1	1.5e-05	89.66	metalloendoproteinase 5-MMP-like [Cucurbita pepo subsp. pepo]	[more]
KAG6602462.1	1.3e-04	86.21	Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022962668.1	1.3e-04	86.21	metalloendoproteinase 2-MMP-like [Cucurbita moschata]	[more]
XP_022991021.1	1.3e-04	82.76	metalloendoproteinase 2-MMP-like [Cucurbita maxima]	[more]

BLAST of Carg04413 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6Y4Q5	3.5e-05	42.86	Stromelysin-1 OS=Canis lupus familiaris OX=9615 GN=MMP3 PE=2 SV=1	[more]
Q28397	5.9e-05	54.29	Stromelysin-1 OS=Equus caballus OX=9796 GN=MMP3 PE=2 SV=1	[more]
P33435	1.3e-04	57.14	Collagenase 3 OS=Mus musculus OX=10090 GN=Mmp13 PE=1 SV=1	[more]
P23097	2.9e-04	58.82	Collagenase 3 (Fragment) OS=Rattus norvegicus OX=10116 GN=Mmp13 PE=1 SV=1	[more]
Q10835	3.9e-04	61.29	Collagenase 3 (Fragment) OS=Xenopus laevis OX=8355 GN=mmp13 PE=2 SV=1	[more]

BLAST of Carg04413 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JPK1	6.2e-05	82.76	metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111487736 PE=...	[more]
A0A6J1HFG1	6.2e-05	86.21	metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111463086 P...	[more]
A0A6S7PL12	1.1e-04	52.83	ZnMc domain-containing protein OS=Lactuca saligna OX=75948 GN=LSAL_LOCUS42559 PE...	[more]
A0A6J1BXD5	3.1e-04	58.70	metalloendoproteinase 2-MMP-like OS=Momordica charantia OX=3673 GN=LOC111006498 ...	[more]
A0A6S7NKS7	3.1e-04	62.86	ZnMc domain-containing protein OS=Lactuca saligna OX=75948 GN=LSAL_LOCUS24722 PE...	[more]

BLAST of Carg04413 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 17..61 e-value: 8.8E-10 score: 38.6
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 9..68 e-value: 6.6E-8 score: 34.2
None	No IPR available	SUPERFAMILY	55486	Metalloproteases ("zincins"), catalytic domain	coord: 24..64

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg04413-RA	Carg04413-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030574	collagen catabolic process
biological_process	GO:0030198	extracellular matrix organization
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity