Carg04403 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04403
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Procollagen-proline 4-dioxygenase
Location	Carg_Chr04: 20582420 .. 20585615 (-)
RNA-Seq Expression	Carg04403
Synteny	Carg04403

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCCCGTTGGTTCCTCACATTCTCCCTTTGCGTTCTGTTCGTCTTCACTGCCTTCGCTCGCTTGCCCCAATCGCTTACGCACAACAAACTGTATGATCCTTTTCCTCTTCTTCTTCTTACTCTTCGGTTTGTTTTCATGATTCCGCTTACGCATGGCTTTGGATTCTGATTTTTCGTGTTTTTTTCTTCGAATTTCAGAGGCGGATCTGCACTTCGGTTGGAGGGGAGTTCATCTCCGCTGATTTTTGATCCAACTCGAGTCACTCAGCTCTCCTGGCAACCTAGGTCATCTCTCTTTCTCAAAATTCGCTTCTCATATACTCTCAAACCTCTCCCTAATAAAATCGTGAGGCTCACGCCAATATGTAACATTGGTTAACTATGTGTTTGACTGTTACAAATGATATCAGAGCCAAACATTAGGCGGTGGCTCTCAAAGGGAGTGTATTATGAGATTCCACATTGATTAGAGAGGATAACGAAACATTCCTTAAGGTGTGGAAACCTCTCCCTACTTCACTCGTTTTAAAACTCTTATAAGTTTTATAAGAGGCGGTGTATTATGAGATAGAGAGGGGACGAAGTATTCCTTAAAGGGTGTGGAAACCTCTACCTAGTTGAAGCATTTTAAAATCGTGAGGCTAACGATAATAGATAACTAGCCAAAGCGAACAACATATGTTATGTGTGGGTTTGGGCGGTTACAAGTGGTATCAAAGCTAGACACTGGGTTAGTGTGCTGCTAGAAATTGGCACCCAAGGGATTGGATTGTGAGATCCCACGTCGGTTAGAGAGGGGACGAAACATTTCTTAAAGAGTGTGCAGAAACTTTTCTCTAGCAAACGCGTTTCAAAACAATAAAGCTAACGGCAATATATAACGAGCAAAAACGGACAATATTTGTCAGCTATGAGTTTGGGCTGTTACAAATGGTATTAGAGCCAGACATCGGTCGGTGTACCAACTATGACGTTGGCCCGAGATCCACGTTAGTTAGAGAGGGGAACACAACATTTCTTAAATATGTGGAAACGTCTCTCTAACAAATACATGTTTTAAAACCGTGAGGTTGATGACTATAGATAACTAGCCAAAGCTAACAATATCGGTTAGTGGTCGGCTTAGCTTGTCCCGTTACATACCCAAACCTGGCTTTAGTTTTTTTTATTGGGGATGTGTTTGTGCAAATGTACAGGGCATTATTATATAAGGGATTTCTATCTGATAAGGAATGCGATCACCTAATCAATCTGGTGAGTGAATAAGGGAATAATTTAGATTTGAATTCAACAGCTCTAAAATGTTTGTTTGATTGTATCATTTTTTGGGAATAGTCAAAGGGAAGGTTAGAGAGGTCGATGGTAGCAGATAACAAGTCCGGTAAGAGAATAAGTAGTGAAGTCCGGACCAGCTCCGGCACGTTCGTGCTGAAGGGGCAGGATGAAATAATTGCTGCCATTGAAGCCAGAATTGCGGCATGGACATTCCTTCCAATAGGTATATGTATATGTATATGTATATGTATATGTATATTTGTATATGTATATTCTGGTTATTAAAGTTACCGTTCTTTGAATCAGAAAATGGAGAGCCCATTCAAATTCTGCACTATGAGAATGGTGAGAAGTATGAACCGCATTTTGATTTTTTTGTGGACGAGGTGAATAAGGAGTTGGGTGGCCACCGAGTAGCCACAGTTTTGATGTATTTATCCAATGTTGAGAAGGGTGGAGAGACCGTGTTTCCACATTCAGAGGTATATGTATATATACATGCCACTGCCTCTGCAGCGTCTCTCTTCTTTATCTCTCTGAAAATTAATAGGCAAGCGTTGTTGCTCTGTTGTTTATGCAGTTGAAAGAGTCTCAAGAAAAGGATGATAGCTGGTCTGATTGTGCTCGAATGGGTTATGCAGGTACTCCTAATTTTTTTTTTAATCCCATCAATAATAAACTTACTTAAGGTGAAACTGAATAATAAACTCAGTTAAGGTGAAACTATCTGATCCAGAGAGAAATTAAATATTATTTATAACAATGTAGAAATATCTTCTTATATGTGTTTTAAAAATTTGAGAAAAACCTGAAAGTGATAGTTCAAAAAGGACCTGTACTTGGGCTGTTTTAAAAATTTGAACGAAAATTAGGAAGAGATAATTTAAAGAAAACAAAATCTACTAACGGTATAATATTTGAATTTGATTAGTTTGAGTTAGTTCCAGTCATTTATTCCATTGTTTTTTAATAGAGTAGAAGGGGAGGGTTTAAAACGTTAGTTTATTTATTTTTAAATATTGAATTAAAATTTATAATGAGTTGAAATTTTGCATTAAAAATACTAAAAAAAGTTTTTTTTTTTTTTTTTAATTTTCTTTTTAAAAAAATTATAGAATTAAATAAAATTTAAACAATTAGAAATATCTAATTCAACCTCACCCATATAATTTTATTTATTTAAATTCAATCCAACTCAAATCATATAATTTTATTTATTTAAATTCAATCCAACTCAAATCAAACTGCATGGATTTTTTTTAACATCATTTTTTACCACAATGTTTTCAAAATTGATGCTGAATTTTGAATTTTGAATTTTGAATTTTGATTTTTTTTTTAAAATTTCAACTCATTTTTGTTTATTGGATTTAGCCAAGAATTCAATGTCTTCCAAAGAAAAGACACAATTTTTCATTATGAAGTTGTATTCTATTTCTGGTGTCCATGTGTTCTAAAGCTTGGGGGTGATGGTTGTTGCAGTTAAACCGCAGAAGGGTGATGCATTGCTGTTCTTCAGCCTCAATGTGGATGGAACCACAAATCCAAGAAGCATGCACGGTAGCTGCCCAGTGATTGAAGGAGAGAAATGGTCTGCAACCAAATGGATTCATGTCAGATCCTTTGATAACCCAATTCTCCCAAACAAGGGCTGCATGGACTTCAACGAAAATTGCCCTTTGTGGGCCGAAAACGGTGAGTGCAAAAACAACCCCAGGTACATGCTGGGCTCTGAAACTGCTTCAGGATACTGTAGGAAGAGTTGCCAAGCCTGCTAAACTACAAACTCCAACAACCAAGTCTCCATTCTGTCATGGTTATCATGTACATAACATTCGACACTAACTAGATATAGCTTTGCTTCTTCTAGTCGACCTTCTCGGTTCCGTTATACAAATAAGGTGGGCGGTCAAATG

mRNA sequence

ATGGATTCCCGTTGGTTCCTCACATTCTCCCTTTGCGTTCTGTTCGTCTTCACTGCCTTCGCTCGCTTGCCCCAATCGCTTACGCACAACAAACTGTATGATCCTTTTCCTCTTCTTCTTCTTACTCTTCGAGGCGGATCTGCACTTCGGTTGGAGGGGAGTTCATCTCCGCTGATTTTTGATCCAACTCGAGTCACTCAGCTCTCCTGGCAACCTAGGGCATTATTATATAAGGGATTTCTATCTGATAAGGAATGCGATCACCTAATCAATCTGTCAAAGGGAAGGTTAGAGAGGTCGATGGTAGCAGATAACAAGTCCGGTAAGAGAATAAGTAGTGAAGTCCGGACCAGCTCCGGCACGTTCGTGCTGAAGGGGCAGGATGAAATAATTGCTGCCATTGAAGCCAGAATTGCGGCATGGACATTCCTTCCAATAGAAAATGGAGAGCCCATTCAAATTCTGCACTATGAGAATGGTGAGAAGTATGAACCGCATTTTGATTTTTTTGTGGACGAGGTGAATAAGGAGTTGGGTGGCCACCGAGTAGCCACAGTTTTGATGTATTTATCCAATGTTGAGAAGGGTGGAGAGACCGTGTTTCCACATTCAGAGTTGAAAGAGTCTCAAGAAAAGGATGATAGCTGGTCTGATTGTGCTCGAATGGGTTATGCAGTTAAACCGCAGAAGGGTGATGCATTGCTGTTCTTCAGCCTCAATGTGGATGGAACCACAAATCCAAGAAGCATGCACGGTAGCTGCCCAGTGATTGAAGGAGAGAAATGGTCTGCAACCAAATGGATTCATGTCAGATCCTTTGATAACCCAATTCTCCCAAACAAGGGCTGCATGGACTTCAACGAAAATTGCCCTTTGTGGGCCGAAAACGGTGAGTGCAAAAACAACCCCAGGTACATGCTGGGCTCTGAAACTGCTTCAGGATACTGTAGGAAGAGTTGCCAAGCCTGCTAAACTACAAACTCCAACAACCAAGTCTCCATTCTGTCATGGTTATCATGTACATAACATTCGACACTAACTAGATATAGCTTTGCTTCTTCTAGTCGACCTTCTCGGTTCCGTTATACAAATAAGGTGGGCGGTCAAATG

Coding sequence (CDS)

ATGGATTCCCGTTGGTTCCTCACATTCTCCCTTTGCGTTCTGTTCGTCTTCACTGCCTTCGCTCGCTTGCCCCAATCGCTTACGCACAACAAACTGTATGATCCTTTTCCTCTTCTTCTTCTTACTCTTCGAGGCGGATCTGCACTTCGGTTGGAGGGGAGTTCATCTCCGCTGATTTTTGATCCAACTCGAGTCACTCAGCTCTCCTGGCAACCTAGGGCATTATTATATAAGGGATTTCTATCTGATAAGGAATGCGATCACCTAATCAATCTGTCAAAGGGAAGGTTAGAGAGGTCGATGGTAGCAGATAACAAGTCCGGTAAGAGAATAAGTAGTGAAGTCCGGACCAGCTCCGGCACGTTCGTGCTGAAGGGGCAGGATGAAATAATTGCTGCCATTGAAGCCAGAATTGCGGCATGGACATTCCTTCCAATAGAAAATGGAGAGCCCATTCAAATTCTGCACTATGAGAATGGTGAGAAGTATGAACCGCATTTTGATTTTTTTGTGGACGAGGTGAATAAGGAGTTGGGTGGCCACCGAGTAGCCACAGTTTTGATGTATTTATCCAATGTTGAGAAGGGTGGAGAGACCGTGTTTCCACATTCAGAGTTGAAAGAGTCTCAAGAAAAGGATGATAGCTGGTCTGATTGTGCTCGAATGGGTTATGCAGTTAAACCGCAGAAGGGTGATGCATTGCTGTTCTTCAGCCTCAATGTGGATGGAACCACAAATCCAAGAAGCATGCACGGTAGCTGCCCAGTGATTGAAGGAGAGAAATGGTCTGCAACCAAATGGATTCATGTCAGATCCTTTGATAACCCAATTCTCCCAAACAAGGGCTGCATGGACTTCAACGAAAATTGCCCTTTGTGGGCCGAAAACGGTGAGTGCAAAAACAACCCCAGGTACATGCTGGGCTCTGAAACTGCTTCAGGATACTGTAGGAAGAGTTGCCAAGCCTGCTAA

Protein sequence

MDSRWFLTFSLCVLFVFTAFARLPQSLTHNKLYDPFPLLLLTLRGGSALRLEGSSSPLIFDPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSEVRTSSGTFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGGHRVATVLMYLSNVEKGGETVFPHSELKESQEKDDSWSDCARMGYAVKPQKGDALLFFSLNVDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCPLWAENGECKNNPRYMLGSETASGYCRKSCQAC

Homology

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BLAST of Carg04403 vs. NCBI nr
Match: KAG7033150.1 (putative prolyl 4-hydroxylase 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 676.8 bits (1745), Expect = 9.3e-191
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0


Query: 1   MDSRWFLTFSLCVLFVFTAFARLPQSLTHNKLYDPFPLLLLTLRGGSALRLEGSSSPLIF 60
           MDSRWFLTFSLCVLFVFTAFARLPQSLTHNKLYDPFPLLLLTLRGGSALRLEGSSSPLIF
Sbjct: 1   MDSRWFLTFSLCVLFVFTAFARLPQSLTHNKLYDPFPLLLLTLRGGSALRLEGSSSPLIF 60

Query: 61  DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSEVRTSSG 120
           DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSEVRTSSG
Sbjct: 61  DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSEVRTSSG 120

Query: 121 TFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG 180
           TFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG
Sbjct: 121 TFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG 180

Query: 181 HRVATVLMYLSNVEKGGETVFPHSELKESQEKDDSWSDCARMGYAVKPQKGDALLFFSLN 240
           HRVATVLMYLSNVEKGGETVFPHSELKESQEKDDSWSDCARMGYAVKPQKGDALLFFSLN
Sbjct: 181 HRVATVLMYLSNVEKGGETVFPHSELKESQEKDDSWSDCARMGYAVKPQKGDALLFFSLN 240

Query: 241 VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCPLWAENGECK 300
           VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCPLWAENGECK
Sbjct: 241 VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCPLWAENGECK 300

Query: 301 NNPRYMLGSETASGYCRKSCQAC 324
           NNPRYMLGSETASGYCRKSCQAC
Sbjct: 301 NNPRYMLGSETASGYCRKSCQAC 323

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BLAST of Carg04403 vs. NCBI nr
Match: XP_022961014.1 (probable prolyl 4-hydroxylase 6 [Cucurbita moschata])

HSP 1 Score: 642.5 bits (1656), Expect = 1.9e-180
Identity = 310/323 (95.98%), Postives = 310/323 (95.98%), Query Frame = 0


Query: 1   MDSRWFLTFSLCVLFVFTAFARLPQSLTHNKLYDPFPLLLLTLRGGSALRLEGSSSPLIF 60
           MDSRWFLTFSLCVLFVFTAFARLPQSLTHNKL            GGSALRLEGSSSPLIF
Sbjct: 1   MDSRWFLTFSLCVLFVFTAFARLPQSLTHNKL------------GGSALRLEGSSSPLIF 60

Query: 61  DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSEVRTSSG 120
           DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSEVRTSSG
Sbjct: 61  DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSEVRTSSG 120

Query: 121 TFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG 180
           TFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG
Sbjct: 121 TFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG 180

Query: 181 HRVATVLMYLSNVEKGGETVFPHSELKESQEKDDSWSDCARMGYAVKPQKGDALLFFSLN 240
           HRVATVLMYLSNVEKGGETVFPHSE KESQEKDDSWSDCARMGYAVKPQKGDALLFFSLN
Sbjct: 181 HRVATVLMYLSNVEKGGETVFPHSEFKESQEKDDSWSDCARMGYAVKPQKGDALLFFSLN 240

Query: 241 VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCPLWAENGECK 300
           VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCPLWAENGECK
Sbjct: 241 VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCPLWAENGECK 300

Query: 301 NNPRYMLGSETASGYCRKSCQAC 324
           NNPRYMLGSETASGYCRKSCQAC
Sbjct: 301 NNPRYMLGSETASGYCRKSCQAC 311

[Close]

BLAST of Carg04403 vs. NCBI nr
Match: KAG6602475.1 (putative prolyl 4-hydroxylase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 638.3 bits (1645), Expect = 3.7e-179
Identity = 307/323 (95.05%), Postives = 311/323 (96.28%), Query Frame = 0


Query: 1   MDSRWFLTFSLCVLFVFTAFARLPQSLTHNKLYDPFPLLLLTLRGGSALRLEGSSSPLIF 60
           MDSRWFLTFSLCVLFVFTAFARLPQSL+HNKL            GGSALRL+GSSSPLIF
Sbjct: 1   MDSRWFLTFSLCVLFVFTAFARLPQSLSHNKL------------GGSALRLKGSSSPLIF 60

Query: 61  DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSEVRTSSG 120
           DPTRVTQLSWQPRALLYKGFLSDKECDHL+NLSKGRLERSMVADN+SGKRISSEVRTSSG
Sbjct: 61  DPTRVTQLSWQPRALLYKGFLSDKECDHLMNLSKGRLERSMVADNESGKRISSEVRTSSG 120

Query: 121 TFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG 180
           TFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG
Sbjct: 121 TFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG 180

Query: 181 HRVATVLMYLSNVEKGGETVFPHSELKESQEKDDSWSDCARMGYAVKPQKGDALLFFSLN 240
           HRVATVLMYLSNVEKGGETVFPHSELKESQEKDDSWSDCARMGYAVKPQKGDALLFFSLN
Sbjct: 181 HRVATVLMYLSNVEKGGETVFPHSELKESQEKDDSWSDCARMGYAVKPQKGDALLFFSLN 240

Query: 241 VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCPLWAENGECK 300
           VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCPLWAENGECK
Sbjct: 241 VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCPLWAENGECK 300

Query: 301 NNPRYMLGSETASGYCRKSCQAC 324
           NNPRYMLGSETASGYCRKSCQAC
Sbjct: 301 NNPRYMLGSETASGYCRKSCQAC 311

[Close]

BLAST of Carg04403 vs. NCBI nr
Match: XP_023523636.1 (probable prolyl 4-hydroxylase 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 632.9 bits (1631), Expect = 1.5e-177
Identity = 303/323 (93.81%), Postives = 308/323 (95.36%), Query Frame = 0


Query: 1   MDSRWFLTFSLCVLFVFTAFARLPQSLTHNKLYDPFPLLLLTLRGGSALRLEGSSSPLIF 60
           MDSRWFLTFSLC LFVFTA ARLPQSLTHNKL            GGSALRL+GSSSPLIF
Sbjct: 1   MDSRWFLTFSLCYLFVFTALARLPQSLTHNKL------------GGSALRLKGSSSPLIF 60

Query: 61  DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSEVRTSSG 120
           DPTRVTQLSWQPRALLYKGFLSDKECDHLINLS+GRLERSMVADNKSGKR+SS+VRTSSG
Sbjct: 61  DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSRGRLERSMVADNKSGKRVSSKVRTSSG 120

Query: 121 TFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG 180
           TFVLKGQDEIIAAIEARIAAWTFLP+ENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG
Sbjct: 121 TFVLKGQDEIIAAIEARIAAWTFLPLENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG 180

Query: 181 HRVATVLMYLSNVEKGGETVFPHSELKESQEKDDSWSDCARMGYAVKPQKGDALLFFSLN 240
           HRVATVLMYLSNVEKGGETVFPHSE KESQEKDDSWSDCARMGYAVKPQKGDALLFFSLN
Sbjct: 181 HRVATVLMYLSNVEKGGETVFPHSEFKESQEKDDSWSDCARMGYAVKPQKGDALLFFSLN 240

Query: 241 VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCPLWAENGECK 300
           VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCPLWAENGECK
Sbjct: 241 VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCPLWAENGECK 300

Query: 301 NNPRYMLGSETASGYCRKSCQAC 324
           NNPRYMLGSETASGYCRKSCQAC
Sbjct: 301 NNPRYMLGSETASGYCRKSCQAC 311

[Close]

BLAST of Carg04403 vs. NCBI nr
Match: XP_022990688.1 (probable prolyl 4-hydroxylase 7 [Cucurbita maxima])

HSP 1 Score: 631.3 bits (1627), Expect = 4.5e-177
Identity = 302/323 (93.50%), Postives = 309/323 (95.67%), Query Frame = 0


Query: 1   MDSRWFLTFSLCVLFVFTAFARLPQSLTHNKLYDPFPLLLLTLRGGSALRLEGSSSPLIF 60
           MDSRWFLTFSLC LFVFTAFARLPQSLTHN+L            GGSALRL+G+SSPLIF
Sbjct: 1   MDSRWFLTFSLCFLFVFTAFARLPQSLTHNEL------------GGSALRLKGNSSPLIF 60

Query: 61  DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSEVRTSSG 120
           DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISS+VRTSSG
Sbjct: 61  DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSKVRTSSG 120

Query: 121 TFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG 180
           TFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG
Sbjct: 121 TFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG 180

Query: 181 HRVATVLMYLSNVEKGGETVFPHSELKESQEKDDSWSDCARMGYAVKPQKGDALLFFSLN 240
           HRVATVLMYLSNVEKGGETVFPHSE KESQEKDDSWSDCARMGYAVKP+KGDALLFFSLN
Sbjct: 181 HRVATVLMYLSNVEKGGETVFPHSEFKESQEKDDSWSDCARMGYAVKPEKGDALLFFSLN 240

Query: 241 VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCPLWAENGECK 300
           VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFN+NCPLWAENGECK
Sbjct: 241 VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNQNCPLWAENGECK 300

Query: 301 NNPRYMLGSETASGYCRKSCQAC 324
           NNPRYMLGSET+SGYCRKSCQAC
Sbjct: 301 NNPRYMLGSETSSGYCRKSCQAC 311

[Close]

BLAST of Carg04403 vs. ExPASy Swiss-Prot
Match: Q8L970 (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 9.9e-119
Identity = 200/324 (61.73%), Postives = 248/324 (76.54%), Query Frame = 0


Query: 1   MDSRWFLTFSLCVLFVFTAFARLP-QSLTHNKLYDPFPLLLLTLRGGSALRLEGSSSPLI 60
           MDSR FL FSLC LF     +  P + LT +             R GS ++++ S+S   
Sbjct: 1   MDSRIFLAFSLCFLFTLPLISSAPNRFLTRSS----------NTRDGSVIKMKTSASSFG 60

Query: 61  FDPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSEVRTSS 120
           FDPTRVTQLSW PR  LY+GFLSD+ECDH I L+KG+LE+SMVADN SG+ + SEVRTSS
Sbjct: 61  FDPTRVTQLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSS 120

Query: 121 GTFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELG 180
           G F+ K QD+I++ +EA++AAWTFLP ENGE +QILHYENG+KYEPHFD+F D+ N ELG
Sbjct: 121 GMFLSKRQDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELG 180

Query: 181 GHRVATVLMYLSNVEKGGETVFPHSELKESQEKDDSWSDCARMGYAVKPQKGDALLFFSL 240
           GHR+ATVLMYLSNVEKGGETVFP  + K +Q KDDSW++CA+ GYAVKP+KGDALLFF+L
Sbjct: 181 GHRIATVLMYLSNVEKGGETVFPMWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFNL 240

Query: 241 NVDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCPLWAENGEC 300
           + + TT+  S+HGSCPV+EGEKWSAT+WIHV+SF+       GCMD N +C  WA+ GEC
Sbjct: 241 HPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCEKWAKAGEC 300

Query: 301 KNNPRYMLGSETASGYCRKSCQAC 324
           + NP YM+GS+   GYCRKSC+AC
Sbjct: 301 QKNPTYMVGSDKDHGYCRKSCKAC 314

[Close]

BLAST of Carg04403 vs. ExPASy Swiss-Prot
Match: F4J0A8 (Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 2.4e-109
Identity = 195/324 (60.19%), Postives = 238/324 (73.46%), Query Frame = 0


Query: 1   MDSRWFLTFSLCVLFVFTAFARLPQSLTHNKLYDPFPLLLLTLRGGSALRLEGSSSPLIF 60
           MDS++FL FSL +L +F+                                   SS     
Sbjct: 1   MDSQYFLAFSLSLLLIFSQI---------------------------------SSFSFSV 60

Query: 61  DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSM-VADNKSGKRISSEVRTSS 120
           DPTR+TQLSW PRA LYKGFLSD+ECDHLI L+KG+LE+SM VAD  SG+   SEVRTSS
Sbjct: 61  DPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGESEDSEVRTSS 120

Query: 121 GTFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELG 180
           G F+ K QD+I+A +EA++AAWTFLP ENGE +QILHYENG+KY+PHFD+F D+   ELG
Sbjct: 121 GMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDYFYDKKALELG 180

Query: 181 GHRVATVLMYLSNVEKGGETVFPHSELKESQEKDDSWSDCARMGYAVKPQKGDALLFFSL 240
           GHR+ATVLMYLSNV KGGETVFP+ + K  Q KDDSWS CA+ GYAVKP+KGDALLFF+L
Sbjct: 181 GHRIATVLMYLSNVTKGGETVFPNWKGKTPQLKDDSWSKCAKQGYAVKPRKGDALLFFNL 240

Query: 241 NVDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCPLWAENGEC 300
           +++GTT+P S+HGSCPVIEGEKWSAT+WIHVRSF    L    C+D +E+C  WA+ GEC
Sbjct: 241 HLNGTTDPNSLHGSCPVIEGEKWSATRWIHVRSFGKKKLV---CVDDHESCQEWADAGEC 288

Query: 301 KNNPRYMLGSETASGYCRKSCQAC 324
           + NP YM+GSET+ G+CRKSC+AC
Sbjct: 301 EKNPMYMVGSETSLGFCRKSCKAC 288

[Close]

BLAST of Carg04403 vs. ExPASy Swiss-Prot
Match: Q8LAN3 (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 4.5e-95
Identity = 160/273 (58.61%), Postives = 207/273 (75.82%), Query Frame = 0


Query: 54  SSSPLIFDPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISS 113
           SSS +  +P++V Q+S +PRA +Y+GFL++ ECDH+++L+K  L+RS VADN SG+   S
Sbjct: 26  SSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSLAKASLKRSAVADNDSGESKFS 85

Query: 114 EVRTSSGTFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDE 173
           EVRTSSGTF+ KG+D I++ IE +I+ WTFLP ENGE IQ+L YE+G+KY+ HFD+F D+
Sbjct: 86  EVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYEHGQKYDAHFDYFHDK 145

Query: 174 VNKELGGHRVATVLMYLSNVEKGGETVFPHSELKESQ---EKDDSWSDCARMGYAVKPQK 233
           VN   GGHR+AT+LMYLSNV KGGETVFP +E+   +   E  +  SDCA+ G AVKP+K
Sbjct: 146 VNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVLSENKEDLSDCAKRGIAVKPRK 205

Query: 234 GDALLFFSLNVDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENC 293
           GDALLFF+L+ D   +P S+HG CPVIEGEKWSATKWIHV SFD  + P+  C D NE+C
Sbjct: 206 GDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIHVDSFDRIVTPSGNCTDMNESC 265

Query: 294 PLWAENGECKNNPRYMLGSETASGYCRKSCQAC 324
             WA  GEC  NP YM+G+    GYCR+SC+AC
Sbjct: 266 ERWAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

[Close]

BLAST of Carg04403 vs. ExPASy Swiss-Prot
Match: F4JAU3 (Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 1.0e-91
Identity = 161/291 (55.33%), Postives = 209/291 (71.82%), Query Frame = 0


Query: 36  FPLLLLTLRGGSALRLEGSSSPLIFDPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKG 95
           F  +LL L   S   +   SS  I +P++V Q+S +PRA +Y+GFL+D ECDHLI+L+K 
Sbjct: 11  FVAILLVLLQSSTCLISSPSS--IINPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKE 70

Query: 96  RLERSMVADNKSGKRISSEVRTSSGTFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQIL 155
            L+RS VADN +G+   S+VRTSSGTF+ KG+D I++ IE +++ WTFLP ENGE +Q+L
Sbjct: 71  NLQRSAVADNDNGESQVSDVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVL 130

Query: 156 HYENGEKYEPHFDFFVDEVNKELGGHRVATVLMYLSNVEKGGETVFPHSE---LKESQEK 215
            YE+G+KY+ HFD+F D+VN   GGHR+ATVL+YLSNV KGGETVFP ++    +   E 
Sbjct: 131 RYEHGQKYDAHFDYFHDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSEN 190

Query: 216 DDSWSDCARMGYAVKPQKGDALLFFSLNVDGTTNPRSMHGSCPVIEGEKWSATKWIHVRS 275
            D  SDCA+ G AVKP+KG+ALLFF+L  D   +P S+HG CPVIEGEKWSATKWIHV S
Sbjct: 191 KDDLSDCAKKGIAVKPKKGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHVDS 250

Query: 276 FDNPILPNKGCMDFNENCPLWAENGECKNNPRYMLGSETASGYCRKSCQAC 324
           FD  +  +  C D NE+C  WA  GEC  NP YM+G+    G CR+SC+AC
Sbjct: 251 FDKILTHDGNCTDVNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

[Close]

BLAST of Carg04403 vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.4e-64
Identity = 113/207 (54.59%), Postives = 157/207 (75.85%), Query Frame = 0


Query: 68  LSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSEVRTSSGTFVLKGQ 127
           LSW+PRA +Y  FLS +EC++LI+L+K  + +S V D+++GK   S VRTSSGTF+ +G+
Sbjct: 79  LSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKSKDSRVRTSSGTFLRRGR 138

Query: 128 DEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGGHRVATVL 187
           D+II  IE RIA +TF+P ++GE +Q+LHYE G+KYEPH+D+FVDE N + GG R+AT+L
Sbjct: 139 DKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDYFVDEFNTKNGGQRMATML 198

Query: 188 MYLSNVEKGGETVFPHSELK-ESQEKDDSWSDCARMGYAVKPQKGDALLFFSLNVDGTTN 247
           MYLS+VE+GGETVFP + +   S    +  S+C + G +VKP+ GDALLF+S+  D T +
Sbjct: 199 MYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSVKPRMGDALLFWSMRPDATLD 258

Query: 248 PRSMHGSCPVIEGEKWSATKWIHVRSF 274
           P S+HG CPVI G KWS+TKW+HV  +
Sbjct: 259 PTSLHGGCPVIRGNKWSSTKWMHVGEY 285

[Close]

BLAST of Carg04403 vs. ExPASy TrEMBL
Match: A0A6J1HCS1 (Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111461644 PE=3 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 9.4e-181
Identity = 310/323 (95.98%), Postives = 310/323 (95.98%), Query Frame = 0


Query: 1   MDSRWFLTFSLCVLFVFTAFARLPQSLTHNKLYDPFPLLLLTLRGGSALRLEGSSSPLIF 60
           MDSRWFLTFSLCVLFVFTAFARLPQSLTHNKL            GGSALRLEGSSSPLIF
Sbjct: 1   MDSRWFLTFSLCVLFVFTAFARLPQSLTHNKL------------GGSALRLEGSSSPLIF 60

Query: 61  DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSEVRTSSG 120
           DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSEVRTSSG
Sbjct: 61  DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSEVRTSSG 120

Query: 121 TFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG 180
           TFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG
Sbjct: 121 TFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG 180

Query: 181 HRVATVLMYLSNVEKGGETVFPHSELKESQEKDDSWSDCARMGYAVKPQKGDALLFFSLN 240
           HRVATVLMYLSNVEKGGETVFPHSE KESQEKDDSWSDCARMGYAVKPQKGDALLFFSLN
Sbjct: 181 HRVATVLMYLSNVEKGGETVFPHSEFKESQEKDDSWSDCARMGYAVKPQKGDALLFFSLN 240

Query: 241 VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCPLWAENGECK 300
           VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCPLWAENGECK
Sbjct: 241 VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCPLWAENGECK 300

Query: 301 NNPRYMLGSETASGYCRKSCQAC 324
           NNPRYMLGSETASGYCRKSCQAC
Sbjct: 301 NNPRYMLGSETASGYCRKSCQAC 311

[Close]

BLAST of Carg04403 vs. ExPASy TrEMBL
Match: A0A6J1JU08 (Procollagen-proline 4-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111487502 PE=3 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 2.2e-177
Identity = 302/323 (93.50%), Postives = 309/323 (95.67%), Query Frame = 0


Query: 1   MDSRWFLTFSLCVLFVFTAFARLPQSLTHNKLYDPFPLLLLTLRGGSALRLEGSSSPLIF 60
           MDSRWFLTFSLC LFVFTAFARLPQSLTHN+L            GGSALRL+G+SSPLIF
Sbjct: 1   MDSRWFLTFSLCFLFVFTAFARLPQSLTHNEL------------GGSALRLKGNSSPLIF 60

Query: 61  DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSEVRTSSG 120
           DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISS+VRTSSG
Sbjct: 61  DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSKVRTSSG 120

Query: 121 TFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG 180
           TFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG
Sbjct: 121 TFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG 180

Query: 181 HRVATVLMYLSNVEKGGETVFPHSELKESQEKDDSWSDCARMGYAVKPQKGDALLFFSLN 240
           HRVATVLMYLSNVEKGGETVFPHSE KESQEKDDSWSDCARMGYAVKP+KGDALLFFSLN
Sbjct: 181 HRVATVLMYLSNVEKGGETVFPHSEFKESQEKDDSWSDCARMGYAVKPEKGDALLFFSLN 240

Query: 241 VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCPLWAENGECK 300
           VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFN+NCPLWAENGECK
Sbjct: 241 VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNQNCPLWAENGECK 300

Query: 301 NNPRYMLGSETASGYCRKSCQAC 324
           NNPRYMLGSET+SGYCRKSCQAC
Sbjct: 301 NNPRYMLGSETSSGYCRKSCQAC 311

[Close]

BLAST of Carg04403 vs. ExPASy TrEMBL
Match: A0A6J1BXN9 (Procollagen-proline 4-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111006412 PE=3 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 1.1e-139
Identity = 242/325 (74.46%), Postives = 272/325 (83.69%), Query Frame = 0


Query: 1   MDSRWFLTFSLCVLFVFTAFARLPQSLTHNKLYDPFPLLLLTLRGGSALRLEGSSSPLIF 60
           MDS  FL+FSLC LFVFTA ARLP    H K+             GS LRL+G  SPLIF
Sbjct: 1   MDSPRFLSFSLCFLFVFTALARLPDMRAHKKI------------SGSVLRLKGEPSPLIF 60

Query: 61  DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSEVRTSSG 120
           DPTRVTQLSWQPRA LYKGFLSDKECDHLI+L+K +LE+SMVADN SGK +SSEVRTSSG
Sbjct: 61  DPTRVTQLSWQPRAFLYKGFLSDKECDHLIDLAKDKLEKSMVADNNSGKSVSSEVRTSSG 120

Query: 121 TFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG 180
            F+ K QDEI+AA+EARIAAWTFLP ENGE IQILHYENG+KYEPHFD+F D+VN+ELGG
Sbjct: 121 MFLHKAQDEIVAAVEARIAAWTFLPAENGESIQILHYENGQKYEPHFDYFHDKVNQELGG 180

Query: 181 HRVATVLMYLSNVEKGGETVFPHSELKESQEKDDSWSDCARMGYAVKPQKGDALLFFSLN 240
           HRVATVLMYLSNVEKGGET+FP+SE KESQEKDDSWSDCAR GYAVK +KGDALLFFSL+
Sbjct: 181 HRVATVLMYLSNVEKGGETIFPNSEFKESQEKDDSWSDCARKGYAVKAKKGDALLFFSLH 240

Query: 241 VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNK--GCMDFNENCPLWAENGE 300
           +D TT+ +S+HGSCPVIEGEKWSATKWIHVRSF+ P  P++   C+D NENC  WA+ GE
Sbjct: 241 LDATTDVKSLHGSCPVIEGEKWSATKWIHVRSFEKPTRPSRRLDCVDENENCASWAKRGE 300

Query: 301 CKNNPRYMLGSETASGYCRKSCQAC 324
           CK NP YM+GSE+A GYCRKSCQAC
Sbjct: 301 CKKNPTYMVGSESALGYCRKSCQAC 313

[Close]

BLAST of Carg04403 vs. ExPASy TrEMBL
Match: A0A1S3C8G4 (Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103498028 PE=3 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 1.1e-136
Identity = 238/324 (73.46%), Postives = 270/324 (83.33%), Query Frame = 0


Query: 1   MDSRWFLTFSLCVLFVFTAFARLPQSLTHNKLYDPFPLLLLTLRGGSALRLEGSSSPLIF 60
           MDSR FL FSLC L VFTAFARLP++      Y            GS LRL+  SSPLIF
Sbjct: 1   MDSRPFLAFSLCFLSVFTAFARLPETRMLKHSYKQ--------STGSVLRLKTDSSPLIF 60

Query: 61  DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSEVRTSSG 120
           DPTRVTQLSWQPRA LYKGFLSD+ECDHLI+L+K +LE+SMVADN+SGK +SSEVRTSSG
Sbjct: 61  DPTRVTQLSWQPRAFLYKGFLSDEECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSG 120

Query: 121 TFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG 180
            F+ K QD+I+A +EARIAAWT LP ENGE IQILHYENG+KYEPHFDFF D+VN+ELGG
Sbjct: 121 MFLRKAQDKIVAGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGG 180

Query: 181 HRVATVLMYLSNVEKGGETVFPHSELKESQEKDDSWSDCARMGYAVKPQKGDALLFFSLN 240
           HR+ATVLMYLSNVEKGGET+FP+SE KESQEKDDSWSDC+R GYAVK QKGDALLFFSL+
Sbjct: 181 HRIATVLMYLSNVEKGGETIFPNSEFKESQEKDDSWSDCSRKGYAVKAQKGDALLFFSLH 240

Query: 241 VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDN-PILPNKGCMDFNENCPLWAENGEC 300
           +D TT+ RS+HGSCPVIEGEKWSATKWIHVRSF+  P +  + C+D NENCP WA+ GEC
Sbjct: 241 LDATTDERSLHGSCPVIEGEKWSATKWIHVRSFEKLPRVSRQDCVDENENCPAWAKRGEC 300

Query: 301 KNNPRYMLGSETASGYCRKSCQAC 324
           K NP YM+GSE A GYCRKSC+AC
Sbjct: 301 KKNPTYMVGSEGALGYCRKSCKAC 316

[Close]

BLAST of Carg04403 vs. ExPASy TrEMBL
Match: A0A0A0KS38 (Procollagen-proline 4-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_5G633280 PE=3 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 1.0e-134
Identity = 235/325 (72.31%), Postives = 266/325 (81.85%), Query Frame = 0


Query: 1   MDSRWFLTFSLCVLFVFTAFARLPQSLTHNKLYDPFPLLLLTLRGGSALRLEGSSSPLIF 60
           MDSR FL FSLC L VFTAFARLP++ TH +              GS LRL+  SSPLIF
Sbjct: 1   MDSRPFLAFSLCFLSVFTAFARLPETRTHKQ------------SSGSVLRLKTDSSPLIF 60

Query: 61  DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSEVRTSSG 120
           DPTRVTQLSWQPRA LYKGFLSD ECDHLI+L+K +LE+SMVADN SGK +SSEVRTSSG
Sbjct: 61  DPTRVTQLSWQPRAFLYKGFLSDAECDHLIDLAKDKLEKSMVADNDSGKSVSSEVRTSSG 120

Query: 121 TFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELGG 180
            F+ K QDE++A +EARIAAWT LP ENGE IQILHYENG+KYEPHFDFF D+VN+ELGG
Sbjct: 121 MFLRKAQDEVVAGVEARIAAWTLLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGG 180

Query: 181 HRVATVLMYLSNVEKGGETVFPHSELKESQEKDDSWSDCARMGYAVKPQKGDALLFFSLN 240
           HR+ATVLMYLSNVEKGGET+FP+SE KESQ KD+SWSDC+R GYAVK QKGDALLFFSLN
Sbjct: 181 HRIATVLMYLSNVEKGGETIFPNSEFKESQAKDESWSDCSRKGYAVKAQKGDALLFFSLN 240

Query: 241 VDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFD--NPILPNKGCMDFNENCPLWAENGE 300
           +D TT+ RS+HGSCPVI GEKWSATKWIHVRSF+     +  +GC+D NENC  WA+ GE
Sbjct: 241 LDATTDERSLHGSCPVIAGEKWSATKWIHVRSFEKITSRVSRQGCVDENENCLAWAKKGE 300

Query: 301 CKNNPRYMLGSETASGYCRKSCQAC 324
           CK NP YM+GS  A GYCRKSC+AC
Sbjct: 301 CKKNPTYMVGSGGALGYCRKSCKAC 313

[Close]

BLAST of Carg04403 vs. TAIR 10
Match: AT3G28480.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 427.9 bits (1099), Expect = 7.0e-120
Identity = 200/324 (61.73%), Postives = 248/324 (76.54%), Query Frame = 0


Query: 1   MDSRWFLTFSLCVLFVFTAFARLP-QSLTHNKLYDPFPLLLLTLRGGSALRLEGSSSPLI 60
           MDSR FL FSLC LF     +  P + LT +             R GS ++++ S+S   
Sbjct: 1   MDSRIFLAFSLCFLFTLPLISSAPNRFLTRSS----------NTRDGSVIKMKTSASSFG 60

Query: 61  FDPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSEVRTSS 120
           FDPTRVTQLSW PR  LY+GFLSD+ECDH I L+KG+LE+SMVADN SG+ + SEVRTSS
Sbjct: 61  FDPTRVTQLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSS 120

Query: 121 GTFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELG 180
           G F+ K QD+I++ +EA++AAWTFLP ENGE +QILHYENG+KYEPHFD+F D+ N ELG
Sbjct: 121 GMFLSKRQDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELG 180

Query: 181 GHRVATVLMYLSNVEKGGETVFPHSELKESQEKDDSWSDCARMGYAVKPQKGDALLFFSL 240
           GHR+ATVLMYLSNVEKGGETVFP  + K +Q KDDSW++CA+ GYAVKP+KGDALLFF+L
Sbjct: 181 GHRIATVLMYLSNVEKGGETVFPMWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFNL 240

Query: 241 NVDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCPLWAENGEC 300
           + + TT+  S+HGSCPV+EGEKWSAT+WIHV+SF+       GCMD N +C  WA+ GEC
Sbjct: 241 HPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCEKWAKAGEC 300

Query: 301 KNNPRYMLGSETASGYCRKSCQAC 324
           + NP YM+GS+   GYCRKSC+AC
Sbjct: 301 QKNPTYMVGSDKDHGYCRKSCKAC 314

[Close]

BLAST of Carg04403 vs. TAIR 10
Match: AT3G28480.2 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 404.8 bits (1039), Expect = 6.4e-113
Identity = 194/332 (58.43%), Postives = 243/332 (73.19%), Query Frame = 0


Query: 1   MDSRWFLTFSLCVLFVFTAFARLP-QSLTHNKLYDPFPLLLLTLRGGSALRLEGSSSPLI 60
           MDSR FL FSLC LF     +  P + LT +             R GS ++++ S+S   
Sbjct: 1   MDSRIFLAFSLCFLFTLPLISSAPNRFLTRSS----------NTRDGSVIKMKTSASSFG 60

Query: 61  FDPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISSE----V 120
           FDPTRVTQLSW PR  LY+GFLSD+ECDH I L+KG+LE+SMVADN SG+ + SE    V
Sbjct: 61  FDPTRVTQLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEDSVSV 120

Query: 121 RTSSGTFVLKGQ----DEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFV 180
              S +F+        D+I++ +EA++AAWTFLP ENGE +QILHYENG+KYEPHFD+F 
Sbjct: 121 VRQSSSFIANMDSLEIDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFH 180

Query: 181 DEVNKELGGHRVATVLMYLSNVEKGGETVFPHSELKESQEKDDSWSDCARMGYAVKPQKG 240
           D+ N ELGGHR+ATVLMYLSNVEKGGETVFP  + K +Q KDDSW++CA+ GYAVKP+KG
Sbjct: 181 DQANLELGGHRIATVLMYLSNVEKGGETVFPMWKGKATQLKDDSWTECAKQGYAVKPRKG 240

Query: 241 DALLFFSLNVDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCP 300
           DALLFF+L+ + TT+  S+HGSCPV+EGEKWSAT+WIHV+SF+       GCMD N +C 
Sbjct: 241 DALLFFNLHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCE 300

Query: 301 LWAENGECKNNPRYMLGSETASGYCRKSCQAC 324
            WA+ GEC+ NP YM+GS+   GYCRKSC+AC
Sbjct: 301 KWAKAGECQKNPTYMVGSDKDHGYCRKSCKAC 322

[Close]

BLAST of Carg04403 vs. TAIR 10
Match: AT3G28490.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 396.7 bits (1018), Expect = 1.7e-110
Identity = 195/324 (60.19%), Postives = 238/324 (73.46%), Query Frame = 0


Query: 1   MDSRWFLTFSLCVLFVFTAFARLPQSLTHNKLYDPFPLLLLTLRGGSALRLEGSSSPLIF 60
           MDS++FL FSL +L +F+                                   SS     
Sbjct: 1   MDSQYFLAFSLSLLLIFSQI---------------------------------SSFSFSV 60

Query: 61  DPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSM-VADNKSGKRISSEVRTSS 120
           DPTR+TQLSW PRA LYKGFLSD+ECDHLI L+KG+LE+SM VAD  SG+   SEVRTSS
Sbjct: 61  DPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGESEDSEVRTSS 120

Query: 121 GTFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDEVNKELG 180
           G F+ K QD+I+A +EA++AAWTFLP ENGE +QILHYENG+KY+PHFD+F D+   ELG
Sbjct: 121 GMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDYFYDKKALELG 180

Query: 181 GHRVATVLMYLSNVEKGGETVFPHSELKESQEKDDSWSDCARMGYAVKPQKGDALLFFSL 240
           GHR+ATVLMYLSNV KGGETVFP+ + K  Q KDDSWS CA+ GYAVKP+KGDALLFF+L
Sbjct: 181 GHRIATVLMYLSNVTKGGETVFPNWKGKTPQLKDDSWSKCAKQGYAVKPRKGDALLFFNL 240

Query: 241 NVDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENCPLWAENGEC 300
           +++GTT+P S+HGSCPVIEGEKWSAT+WIHVRSF    L    C+D +E+C  WA+ GEC
Sbjct: 241 HLNGTTDPNSLHGSCPVIEGEKWSATRWIHVRSFGKKKLV---CVDDHESCQEWADAGEC 288

Query: 301 KNNPRYMLGSETASGYCRKSCQAC 324
           + NP YM+GSET+ G+CRKSC+AC
Sbjct: 301 EKNPMYMVGSETSLGFCRKSCKAC 288

[Close]

BLAST of Carg04403 vs. TAIR 10
Match: AT5G18900.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 349.4 bits (895), Expect = 3.2e-96
Identity = 160/273 (58.61%), Postives = 207/273 (75.82%), Query Frame = 0


Query: 54  SSSPLIFDPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKGRLERSMVADNKSGKRISS 113
           SSS +  +P++V Q+S +PRA +Y+GFL++ ECDH+++L+K  L+RS VADN SG+   S
Sbjct: 26  SSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSLAKASLKRSAVADNDSGESKFS 85

Query: 114 EVRTSSGTFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQILHYENGEKYEPHFDFFVDE 173
           EVRTSSGTF+ KG+D I++ IE +I+ WTFLP ENGE IQ+L YE+G+KY+ HFD+F D+
Sbjct: 86  EVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYEHGQKYDAHFDYFHDK 145

Query: 174 VNKELGGHRVATVLMYLSNVEKGGETVFPHSELKESQ---EKDDSWSDCARMGYAVKPQK 233
           VN   GGHR+AT+LMYLSNV KGGETVFP +E+   +   E  +  SDCA+ G AVKP+K
Sbjct: 146 VNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVLSENKEDLSDCAKRGIAVKPRK 205

Query: 234 GDALLFFSLNVDGTTNPRSMHGSCPVIEGEKWSATKWIHVRSFDNPILPNKGCMDFNENC 293
           GDALLFF+L+ D   +P S+HG CPVIEGEKWSATKWIHV SFD  + P+  C D NE+C
Sbjct: 206 GDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIHVDSFDRIVTPSGNCTDMNESC 265

Query: 294 PLWAENGECKNNPRYMLGSETASGYCRKSCQAC 324
             WA  GEC  NP YM+G+    GYCR+SC+AC
Sbjct: 266 ERWAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

[Close]

BLAST of Carg04403 vs. TAIR 10
Match: AT3G06300.1 (P4H isoform 2 )

HSP 1 Score: 338.2 bits (866), Expect = 7.3e-93
Identity = 161/291 (55.33%), Postives = 209/291 (71.82%), Query Frame = 0


Query: 36  FPLLLLTLRGGSALRLEGSSSPLIFDPTRVTQLSWQPRALLYKGFLSDKECDHLINLSKG 95
           F  +LL L   S   +   SS  I +P++V Q+S +PRA +Y+GFL+D ECDHLI+L+K 
Sbjct: 11  FVAILLVLLQSSTCLISSPSS--IINPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKE 70

Query: 96  RLERSMVADNKSGKRISSEVRTSSGTFVLKGQDEIIAAIEARIAAWTFLPIENGEPIQIL 155
            L+RS VADN +G+   S+VRTSSGTF+ KG+D I++ IE +++ WTFLP ENGE +Q+L
Sbjct: 71  NLQRSAVADNDNGESQVSDVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVL 130

Query: 156 HYENGEKYEPHFDFFVDEVNKELGGHRVATVLMYLSNVEKGGETVFPHSE---LKESQEK 215
            YE+G+KY+ HFD+F D+VN   GGHR+ATVL+YLSNV KGGETVFP ++    +   E 
Sbjct: 131 RYEHGQKYDAHFDYFHDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSEN 190

Query: 216 DDSWSDCARMGYAVKPQKGDALLFFSLNVDGTTNPRSMHGSCPVIEGEKWSATKWIHVRS 275
            D  SDCA+ G AVKP+KG+ALLFF+L  D   +P S+HG CPVIEGEKWSATKWIHV S
Sbjct: 191 KDDLSDCAKKGIAVKPKKGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHVDS 250

Query: 276 FDNPILPNKGCMDFNENCPLWAENGECKNNPRYMLGSETASGYCRKSCQAC 324
           FD  +  +  C D NE+C  WA  GEC  NP YM+G+    G CR+SC+AC
Sbjct: 251 FDKILTHDGNCTDVNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

The following BLAST results are available for this feature:

BLAST of Carg04403 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7033150.1	9.3e-191	100.00	putative prolyl 4-hydroxylase 7, partial [Cucurbita argyrosperma subsp. argyrosp...	[more]
XP_022961014.1	1.9e-180	95.98	probable prolyl 4-hydroxylase 6 [Cucurbita moschata]	[more]
KAG6602475.1	3.7e-179	95.05	putative prolyl 4-hydroxylase 7, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023523636.1	1.5e-177	93.81	probable prolyl 4-hydroxylase 7 [Cucurbita pepo subsp. pepo]	[more]
XP_022990688.1	4.5e-177	93.50	probable prolyl 4-hydroxylase 7 [Cucurbita maxima]	[more]

BLAST of Carg04403 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L970	9.9e-119	61.73	Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=...	[more]
F4J0A8	2.4e-109	60.19	Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=...	[more]
Q8LAN3	4.5e-95	58.61	Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=...	[more]
F4JAU3	1.0e-91	55.33	Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1	[more]
Q9LN20	1.4e-64	54.59	Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=...	[more]

BLAST of Carg04403 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HCS1	9.4e-181	95.98	Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111461644 ...	[more]
A0A6J1JU08	2.2e-177	93.50	Procollagen-proline 4-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111487502 PE...	[more]
A0A6J1BXN9	1.1e-139	74.46	Procollagen-proline 4-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111006412...	[more]
A0A1S3C8G4	1.1e-136	73.46	Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103498028 PE=3 S...	[more]
A0A0A0KS38	1.0e-134	72.31	Procollagen-proline 4-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_5G633280 PE=...	[more]

BLAST of Carg04403 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G28480.1	7.0e-120	61.73	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G28480.2	6.4e-113	58.43	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G28490.1	1.7e-110	60.19	Oxoglutarate/iron-dependent oxygenase	[more]
AT5G18900.1	3.2e-96	58.61	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G06300.1	7.3e-93	55.33	P4H isoform 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006620	Prolyl 4-hydroxylase, alpha subunit	SMART	SM00702	p4hc	coord: 72..269 e-value: 4.0E-57 score: 205.8
IPR003582	ShKT domain	SMART	SM00254	ShkT_1	coord: 282..323 e-value: 0.0014 score: 27.9
IPR003582	ShKT domain	PFAM	PF01549	ShK	coord: 282..323 e-value: 3.2E-4 score: 21.2
IPR003582	ShKT domain	PROSITE	PS51670	SHKT	coord: 283..323 score: 8.161264
IPR044862	Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domain	PFAM	PF13640	2OG-FeII_Oxy_3	coord: 153..269 e-value: 8.8E-20 score: 71.4
None	No IPR available	GENE3D	2.60.120.620	q2cbj1_9rhob like domain	coord: 64..270 e-value: 1.7E-76 score: 258.5
None	No IPR available	PANTHER	PTHR10869:SF140	OS03G0803500 PROTEIN	coord: 52..323
IPR045054	Prolyl 4-hydroxylase	PANTHER	PTHR10869	PROLYL 4-HYDROXYLASE ALPHA SUBUNIT	coord: 52..323
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 148..270 score: 12.358149

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg04403-RA	Carg04403-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018401	peptidyl-proline hydroxylation to 4-hydroxy-L-proline
cellular_component	GO:0005789	endoplasmic reticulum membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0031418	L-ascorbic acid binding
molecular_function	GO:0004656	procollagen-proline 4-dioxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen