Carg04394 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04394
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Protein kinase domain-containing protein
Location	Carg_Chr04: 20649534 .. 20650685 (+)
RNA-Seq Expression	Carg04394
Synteny	Carg04394

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTTGGCACCTAATGGCTCGAAGGTCGAGACGTGGATTGAGGGCAAGTCAAGAACAGCTAGCGAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGAAAACGAGTTGGAGAAGGAGGAGACTGGATGTTGGGTTAGGTTGAAGTTCATTAGGAGTTGCATTCCTTCTAGATCAAAGGTTGATAGTTCTTTAAGTGGATCAAAGGTCGATAGCTTCATTAGTGGCACCACTACCCATTGCGGTAAACTTCTTGTTCTTTTGGTTTTTGTTTGTATGAGTTAACATAATTCTGAAACGTATTTAGTCTCTTAGTGGATTGAGAAGATTATCCTCATCTTTGAGCTGTTCTTACTTTGTGTTATCATAGAATTTGGAGCATGTGAAGCTAATAACTAAGTTCAAGTGGTGATTAGGTCACGGGTTTACTAAATTAAATTCCCTAGCAATGCAATTTTAAGCGTATCCTGTATTTAAAGCTTGGCAGTACTATGTGAATTGAGATGCCAGCTTTTAAAAGTGATAGATTATTTAACTCGAAAGTGAAGCGTCTGCAAGTTCTATTGAGTTTTGAAAAGCTTCTATATTCATGTTTCTCTGCAATGTTCTTTTGGCATATTGTACTTGATTTCAATACATTTAATACACAAACCAGGGAGTGAGGACTTAACTTTTAGAAGGTAACAAGGACATTATGGATTCAAATGTGCTGGTCATATAGAACAAGGGGAATAAGTGATAGATGTTTCTAAAATCTGATTAATTGGTATTCTTATATTTGTAAATGTTGCTTCTAACTATGTGTAACTAGCAGATAGTAAATCTAAACTCGATGAAAAGAAAGTTATTCTGTTTACTGCATTGGGGTCATCAGCGACAACTGATAATGCTGAAAGTAGCTCGTCCACGCCCAAGCTTAGTGCCGAGCTGAAGGTTGCTTCTCGGCTTCGTAAGTTCACTTTTAATGAGCTTAAGTTAGCCACAAGAAATTTCAGACCTGAGAGCCTTCTTGGCGAGGGGGGTTTTGGTTGTGTTTTCAAAGGATGGATTGAGGAGAATGGAACTGCTCCTGCTAAACCGGGAACTGGGCTTACAGTTGCAGTCAAGACTCTTAATCATGACGGTCTACAAGGTCATAAAGAGTGGATGGTATGA

mRNA sequence

ATGGGTTTGGCACCTAATGGCTCGAAGGTCGAGACGTGGATTGAGGGCAAGTCAAGAACAGCTAGCGAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGAAAACGAGTTGGAGAAGGAGGAGACTGGATGTTGGGTTAGGTTGAAGTTCATTAGGAGTTGCATTCCTTCTAGATCAAAGGTTGATAGTTCTTTAAGTGGATCAAAGGTCGATAGCTTCATTAGTGGCACCACTACCCATTGCGATAGTAAATCTAAACTCGATGAAAAGAAAGTTATTCTGTTTACTGCATTGGGGTCATCAGCGACAACTGATAATGCTGAAAGTAGCTCGTCCACGCCCAAGCTTAGTGCCGAGCTGAAGGTTGCTTCTCGGCTTCGTAAGTTCACTTTTAATGAGCTTAAGTTAGCCACAAGAAATTTCAGACCTGAGAGCCTTCTTGGCGAGGGGGGTTTTGGTTGTGTTTTCAAAGGATGGATTGAGGAGAATGGAACTGCTCCTGCTAAACCGGGAACTGGGCTTACAGTTGCAGTCAAGACTCTTAATCATGACGGTCTACAAGGTCATAAAGAGTGGATGGTATGA

Coding sequence (CDS)

ATGGGTTTGGCACCTAATGGCTCGAAGGTCGAGACGTGGATTGAGGGCAAGTCAAGAACAGCTAGCGAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGAAAACGAGTTGGAGAAGGAGGAGACTGGATGTTGGGTTAGGTTGAAGTTCATTAGGAGTTGCATTCCTTCTAGATCAAAGGTTGATAGTTCTTTAAGTGGATCAAAGGTCGATAGCTTCATTAGTGGCACCACTACCCATTGCGATAGTAAATCTAAACTCGATGAAAAGAAAGTTATTCTGTTTACTGCATTGGGGTCATCAGCGACAACTGATAATGCTGAAAGTAGCTCGTCCACGCCCAAGCTTAGTGCCGAGCTGAAGGTTGCTTCTCGGCTTCGTAAGTTCACTTTTAATGAGCTTAAGTTAGCCACAAGAAATTTCAGACCTGAGAGCCTTCTTGGCGAGGGGGGTTTTGGTTGTGTTTTCAAAGGATGGATTGAGGAGAATGGAACTGCTCCTGCTAAACCGGGAACTGGGCTTACAGTTGCAGTCAAGACTCTTAATCATGACGGTCTACAAGGTCATAAAGAGTGGATGGTATGA

Protein sequence

MGLAPNGSKVETWIEGKSRTASEKKKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKVDSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELKVASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTLNHDGLQGHKEWMV

Homology

[Close]

BLAST of Carg04394 vs. NCBI nr
Match: KAG7033158.1 (putative serine/threonine-protein kinase PIX7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 377.1 bits (967), Expect = 9.1e-101
Identity = 193/193 (100.00%), Postives = 193/193 (100.00%), Query Frame = 0


Query: 1   MGLAPNGSKVETWIEGKSRTASEKKKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKV 60
           MGLAPNGSKVETWIEGKSRTASEKKKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKV
Sbjct: 1   MGLAPNGSKVETWIEGKSRTASEKKKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKV 60

Query: 61  DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK 120
           DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK
Sbjct: 61  DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK 120

Query: 121 VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL 180
           VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL
Sbjct: 121 VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL 180

Query: 181 NHDGLQGHKEWMV 194
           NHDGLQGHKEWMV
Sbjct: 181 NHDGLQGHKEWMV 193

[Close]

BLAST of Carg04394 vs. NCBI nr
Match: XP_022962548.1 (probable serine/threonine-protein kinase PIX7 isoform X1 [Cucurbita moschata])

HSP 1 Score: 375.6 bits (963), Expect = 2.7e-100
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MGLAPNGSKVETWIEGKSRTASEKKKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKV 60
           MGLAPNGSKVETWIEGKSRTASEKKKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKV
Sbjct: 1   MGLAPNGSKVETWIEGKSRTASEKKKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKV 60

Query: 61  DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK 120
           DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK
Sbjct: 61  DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK 120

Query: 121 VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL 180
           VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL
Sbjct: 121 VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL 180

Query: 181 NHDGLQGHKEWM 193
           NHDGLQGHKEWM
Sbjct: 181 NHDGLQGHKEWM 192

[Close]

BLAST of Carg04394 vs. NCBI nr
Match: XP_022962554.1 (probable serine/threonine-protein kinase PIX7 isoform X2 [Cucurbita moschata])

HSP 1 Score: 375.6 bits (963), Expect = 2.7e-100
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MGLAPNGSKVETWIEGKSRTASEKKKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKV 60
           MGLAPNGSKVETWIEGKSRTASEKKKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKV
Sbjct: 1   MGLAPNGSKVETWIEGKSRTASEKKKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKV 60

Query: 61  DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK 120
           DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK
Sbjct: 61  DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK 120

Query: 121 VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL 180
           VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL
Sbjct: 121 VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL 180

Query: 181 NHDGLQGHKEWM 193
           NHDGLQGHKEWM
Sbjct: 181 NHDGLQGHKEWM 192

[Close]

BLAST of Carg04394 vs. NCBI nr
Match: KAG6602482.1 (putative serine/threonine-protein kinase PIX7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 369.8 bits (948), Expect = 1.5e-98
Identity = 191/192 (99.48%), Postives = 191/192 (99.48%), Query Frame = 0


Query: 1   MGLAPNGSKVETWIEGKSRTASEKKKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKV 60
           MGLAPNGSKVETWIEGKSRTASE KKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKV
Sbjct: 1   MGLAPNGSKVETWIEGKSRTASE-KKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKV 60

Query: 61  DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK 120
           DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK
Sbjct: 61  DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK 120

Query: 121 VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL 180
           VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL
Sbjct: 121 VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL 180

Query: 181 NHDGLQGHKEWM 193
           NHDGLQGHKEWM
Sbjct: 181 NHDGLQGHKEWM 191

[Close]

BLAST of Carg04394 vs. NCBI nr
Match: XP_022991185.1 (probable serine/threonine-protein kinase PIX7 isoform X1 [Cucurbita maxima])

HSP 1 Score: 367.5 bits (942), Expect = 7.2e-98
Identity = 190/192 (98.96%), Postives = 190/192 (98.96%), Query Frame = 0


Query: 1   MGLAPNGSKVETWIEGKSRTASEKKKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKV 60
           MGLAPNGSKVETWIEGKSRTASE KKKKKKKKENELEK ETGCWVRLKFIRSCIPSRSKV
Sbjct: 1   MGLAPNGSKVETWIEGKSRTASE-KKKKKKKKENELEKAETGCWVRLKFIRSCIPSRSKV 60

Query: 61  DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK 120
           DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK
Sbjct: 61  DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK 120

Query: 121 VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL 180
           VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL
Sbjct: 121 VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL 180

Query: 181 NHDGLQGHKEWM 193
           NHDGLQGHKEWM
Sbjct: 181 NHDGLQGHKEWM 191

[Close]

BLAST of Carg04394 vs. ExPASy Swiss-Prot
Match: Q9LFP7 (Serine/threonine-protein kinase PBL34 OS=Arabidopsis thaliana OX=3702 GN=PBL34 PE=1 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 3.8e-49
Identity = 113/200 (56.50%), Postives = 135/200 (67.50%), Query Frame = 0


Query: 1   MGLAPNGSKVETWIEGKSRTASEKKKKK-----KKKKENELEKEETGCWVRLKFIRSCIP 60
           MGL    +K     E    T ++  KKK     K + E E E E +GCWV+ +F+  CIP
Sbjct: 1   MGLDAVKAKGNWKSEKPKETENKNHKKKNGDDNKSRNEEEEEGEASGCWVKFRFMIGCIP 60

Query: 61  SRSKVD---SSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSST 120
           S+S +D   SS+ GS        T T  +SKS  ++        + S+ TT NAESSSST
Sbjct: 61  SKSDLDASSSSIYGSNC------TVTTMESKSANEKSNDQPVGQVSSTTTTSNAESSSST 120

Query: 121 PKLSAELKVASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTG 180
           P +S EL ++S LRKFTFN+LKL+TRNFRPESLLGEGGFGCVFKGWIEENGTAP KPGTG
Sbjct: 121 PVISEELNISSHLRKFTFNDLKLSTRNFRPESLLGEGGFGCVFKGWIEENGTAPVKPGTG 180

Query: 181 LTVAVKTLNHDGLQGHKEWM 193
           LTVAVKTLN DGLQGHKEW+
Sbjct: 181 LTVAVKTLNPDGLQGHKEWL 194

[Close]

BLAST of Carg04394 vs. ExPASy Swiss-Prot
Match: Q9SRH7 (Serine/threonine-protein kinase PBL35 OS=Arabidopsis thaliana OX=3702 GN=PBL35 PE=1 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 5.1e-46
Identity = 103/190 (54.21%), Postives = 131/190 (68.95%), Query Frame = 0


Query: 10  VETWIEGKSRTASEKKKKKKKKKEN-------ELEKEETGCWVRLKFIRSCIPSRSKVDS 69
           +E W + K+   +EKKKKK+++K+N         E+E  GCWV+ ++I  C  S S V++
Sbjct: 9   MENW-QSKTSNENEKKKKKRRRKKNNNVRNSEHYEEEANGCWVKFRYIVCCASSTSDVET 68

Query: 70  SLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELKVA 129
           SL+ S         T+   S+S + +        + S+ TT NAESS STP +S EL + 
Sbjct: 69  SLTLS---------TSTVGSQSAIVQSNDQPVGPVSSTTTTSNAESSLSTPIISEELNIY 128

Query: 130 SRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTLNH 189
           S L+KF+F +LKLATRNFRPESLLGEGGFGCVFKGW+EENGTAP KPGTGLTVAVKTLN 
Sbjct: 129 SHLKKFSFIDLKLATRNFRPESLLGEGGFGCVFKGWVEENGTAPVKPGTGLTVAVKTLNP 188

Query: 190 DGLQGHKEWM 193
           DGLQGHKEW+
Sbjct: 189 DGLQGHKEWL 188

[Close]

BLAST of Carg04394 vs. ExPASy Swiss-Prot
Match: F4J0D2 (Serine/threonine-protein kinase PBL36 OS=Arabidopsis thaliana OX=3702 GN=PBL36 PE=1 SV=2)

HSP 1 Score: 181.0 bits (458), Expect = 1.3e-44
Identity = 103/180 (57.22%), Postives = 121/180 (67.22%), Query Frame = 0


Query: 16  GKSRTASEKKKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKVDSSLSGSKVDSFISG 75
           G  +    K   KKK  + + ++ E+G W R KFI SCI SRSKVDSS++ + V      
Sbjct: 15  GVEKGEKGKMMSKKKNVKKDGDESESGFWFRFKFIFSCISSRSKVDSSMNATAV------ 74

Query: 76  TTTHCDSKSKLDEKKVILFTALGSSATTDN---AESSSSTPKLSAELKVASRLRKFTFNE 135
                     + E K ++    G  A T +   AES SSTP +S ELK +S+LR F FN+
Sbjct: 75  ----------IAEPKKVIEKLEGHPAPTKDTGCAESGSSTPLMSGELKYSSKLRIFMFND 134

Query: 136 LKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTLNHDGLQGHKEWM 193
           LKLATRNFRPESLLGEGGFGCVFKGWIEENGTAP KPGTGLTVAVKTLN DGLQGHKEW+
Sbjct: 135 LKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPVKPGTGLTVAVKTLNPDGLQGHKEWL 178

[Close]

BLAST of Carg04394 vs. ExPASy Swiss-Prot
Match: O48814 (Serine/threonine-protein kinase BIK1 OS=Arabidopsis thaliana OX=3702 GN=BIK1 PE=1 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.3e-25
Identity = 50/94 (53.19%), Postives = 72/94 (76.60%), Query Frame = 0


Query: 99  SSATTDNAESSSSTPKLSAELKVASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGW 158
           S ++  ++ + ++  K   E+  ++ ++ FTFNELKLATRNFRP+S++GEGGFGCVFKGW
Sbjct: 26  SLSSRKSSSTVAAAQKTEGEILSSTPVKSFTFNELKLATRNFRPDSVIGEGGFGCVFKGW 85

Query: 159 IEENGTAPAKPGTGLTVAVKTLNHDGLQGHKEWM 193
           ++E+   P KPGTGL +AVK LN +G QGH+EW+
Sbjct: 86  LDESTLTPTKPGTGLVIAVKKLNQEGFQGHREWL 119

[Close]

BLAST of Carg04394 vs. ExPASy Swiss-Prot
Match: P43293 (Probable serine/threonine-protein kinase PBL11 OS=Arabidopsis thaliana OX=3702 GN=PBL11 PE=1 SV=2)

HSP 1 Score: 115.2 bits (287), Expect = 8.5e-25
Identity = 49/85 (57.65%), Postives = 66/85 (77.65%), Query Frame = 0


Query: 108 SSSSTPKLSAELKVASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPA 167
           S S  P+   E+   + L+ F+ +ELK ATRNFRP+S++GEGGFGCVFKGWI+E+  AP+
Sbjct: 36  SFSYMPRTEGEILQNANLKNFSLSELKSATRNFRPDSVVGEGGFGCVFKGWIDESSLAPS 95

Query: 168 KPGTGLTVAVKTLNHDGLQGHKEWM 193
           KPGTG+ +AVK LN +G QGH+EW+
Sbjct: 96  KPGTGIVIAVKRLNQEGFQGHREWL 120

[Close]

BLAST of Carg04394 vs. ExPASy TrEMBL
Match: A0A6J1HF39 (probable serine/threonine-protein kinase PIX7 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462942 PE=3 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 1.3e-100
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MGLAPNGSKVETWIEGKSRTASEKKKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKV 60
           MGLAPNGSKVETWIEGKSRTASEKKKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKV
Sbjct: 1   MGLAPNGSKVETWIEGKSRTASEKKKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKV 60

Query: 61  DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK 120
           DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK
Sbjct: 61  DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK 120

Query: 121 VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL 180
           VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL
Sbjct: 121 VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL 180

Query: 181 NHDGLQGHKEWM 193
           NHDGLQGHKEWM
Sbjct: 181 NHDGLQGHKEWM 192

[Close]

BLAST of Carg04394 vs. ExPASy TrEMBL
Match: A0A6J1HHF4 (probable serine/threonine-protein kinase PIX7 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462942 PE=3 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 1.3e-100
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MGLAPNGSKVETWIEGKSRTASEKKKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKV 60
           MGLAPNGSKVETWIEGKSRTASEKKKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKV
Sbjct: 1   MGLAPNGSKVETWIEGKSRTASEKKKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKV 60

Query: 61  DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK 120
           DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK
Sbjct: 61  DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK 120

Query: 121 VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL 180
           VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL
Sbjct: 121 VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL 180

Query: 181 NHDGLQGHKEWM 193
           NHDGLQGHKEWM
Sbjct: 181 NHDGLQGHKEWM 192

[Close]

BLAST of Carg04394 vs. ExPASy TrEMBL
Match: A0A6J1JS65 (probable serine/threonine-protein kinase PIX7 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111487857 PE=3 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 3.5e-98
Identity = 190/192 (98.96%), Postives = 190/192 (98.96%), Query Frame = 0


Query: 1   MGLAPNGSKVETWIEGKSRTASEKKKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKV 60
           MGLAPNGSKVETWIEGKSRTASE KKKKKKKKENELEK ETGCWVRLKFIRSCIPSRSKV
Sbjct: 1   MGLAPNGSKVETWIEGKSRTASE-KKKKKKKKENELEKAETGCWVRLKFIRSCIPSRSKV 60

Query: 61  DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK 120
           DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK
Sbjct: 61  DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK 120

Query: 121 VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL 180
           VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL
Sbjct: 121 VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL 180

Query: 181 NHDGLQGHKEWM 193
           NHDGLQGHKEWM
Sbjct: 181 NHDGLQGHKEWM 191

[Close]

BLAST of Carg04394 vs. ExPASy TrEMBL
Match: A0A6J1JQ16 (probable serine/threonine-protein kinase PIX7 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111487857 PE=3 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 3.5e-98
Identity = 190/192 (98.96%), Postives = 190/192 (98.96%), Query Frame = 0


Query: 1   MGLAPNGSKVETWIEGKSRTASEKKKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKV 60
           MGLAPNGSKVETWIEGKSRTASE KKKKKKKKENELEK ETGCWVRLKFIRSCIPSRSKV
Sbjct: 1   MGLAPNGSKVETWIEGKSRTASE-KKKKKKKKENELEKAETGCWVRLKFIRSCIPSRSKV 60

Query: 61  DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK 120
           DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK
Sbjct: 61  DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK 120

Query: 121 VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL 180
           VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL
Sbjct: 121 VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL 180

Query: 181 NHDGLQGHKEWM 193
           NHDGLQGHKEWM
Sbjct: 181 NHDGLQGHKEWM 191

[Close]

BLAST of Carg04394 vs. ExPASy TrEMBL
Match: A0A0A0KXB4 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G635410 PE=3 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 5.4e-91
Identity = 180/192 (93.75%), Postives = 183/192 (95.31%), Query Frame = 0


Query: 1   MGLAPNGSKVETWIEGKSRTASEKKKKKKKKKENELEKEETGCWVRLKFIRSCIPSRSKV 60
           MGLAPN SKVETWIEGKSRTASE    KKKKKENELE EETGCWV+LKFI SCIPSRSKV
Sbjct: 1   MGLAPNDSKVETWIEGKSRTASE----KKKKKENELE-EETGCWVKLKFIGSCIPSRSKV 60

Query: 61  DSSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELK 120
           DSSLSGSKVDSFISGT+THCDSKSKLDEK+ IL TALGSSATTDNAESSSSTPKLSAELK
Sbjct: 61  DSSLSGSKVDSFISGTSTHCDSKSKLDEKRDILATALGSSATTDNAESSSSTPKLSAELK 120

Query: 121 VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL 180
           VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL
Sbjct: 121 VASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTL 180

Query: 181 NHDGLQGHKEWM 193
           NHDGLQGHKEWM
Sbjct: 181 NHDGLQGHKEWM 187

[Close]

BLAST of Carg04394 vs. TAIR 10
Match: AT5G15080.1 (Protein kinase superfamily protein )

HSP 1 Score: 196.1 bits (497), Expect = 2.7e-50
Identity = 113/200 (56.50%), Postives = 135/200 (67.50%), Query Frame = 0


Query: 1   MGLAPNGSKVETWIEGKSRTASEKKKKK-----KKKKENELEKEETGCWVRLKFIRSCIP 60
           MGL    +K     E    T ++  KKK     K + E E E E +GCWV+ +F+  CIP
Sbjct: 1   MGLDAVKAKGNWKSEKPKETENKNHKKKNGDDNKSRNEEEEEGEASGCWVKFRFMIGCIP 60

Query: 61  SRSKVD---SSLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSST 120
           S+S +D   SS+ GS        T T  +SKS  ++        + S+ TT NAESSSST
Sbjct: 61  SKSDLDASSSSIYGSNC------TVTTMESKSANEKSNDQPVGQVSSTTTTSNAESSSST 120

Query: 121 PKLSAELKVASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTG 180
           P +S EL ++S LRKFTFN+LKL+TRNFRPESLLGEGGFGCVFKGWIEENGTAP KPGTG
Sbjct: 121 PVISEELNISSHLRKFTFNDLKLSTRNFRPESLLGEGGFGCVFKGWIEENGTAPVKPGTG 180

Query: 181 LTVAVKTLNHDGLQGHKEWM 193
           LTVAVKTLN DGLQGHKEW+
Sbjct: 181 LTVAVKTLNPDGLQGHKEWL 194

[Close]

BLAST of Carg04394 vs. TAIR 10
Match: AT3G01300.1 (Protein kinase superfamily protein )

HSP 1 Score: 185.7 bits (470), Expect = 3.6e-47
Identity = 103/190 (54.21%), Postives = 131/190 (68.95%), Query Frame = 0


Query: 10  VETWIEGKSRTASEKKKKKKKKKEN-------ELEKEETGCWVRLKFIRSCIPSRSKVDS 69
           +E W + K+   +EKKKKK+++K+N         E+E  GCWV+ ++I  C  S S V++
Sbjct: 9   MENW-QSKTSNENEKKKKKRRRKKNNNVRNSEHYEEEANGCWVKFRYIVCCASSTSDVET 68

Query: 70  SLSGSKVDSFISGTTTHCDSKSKLDEKKVILFTALGSSATTDNAESSSSTPKLSAELKVA 129
           SL+ S         T+   S+S + +        + S+ TT NAESS STP +S EL + 
Sbjct: 69  SLTLS---------TSTVGSQSAIVQSNDQPVGPVSSTTTTSNAESSLSTPIISEELNIY 128

Query: 130 SRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTLNH 189
           S L+KF+F +LKLATRNFRPESLLGEGGFGCVFKGW+EENGTAP KPGTGLTVAVKTLN 
Sbjct: 129 SHLKKFSFIDLKLATRNFRPESLLGEGGFGCVFKGWVEENGTAPVKPGTGLTVAVKTLNP 188

Query: 190 DGLQGHKEWM 193
           DGLQGHKEW+
Sbjct: 189 DGLQGHKEWL 188

[Close]

BLAST of Carg04394 vs. TAIR 10
Match: AT3G28690.2 (Protein kinase superfamily protein )

HSP 1 Score: 183.7 bits (465), Expect = 1.4e-46
Identity = 101/168 (60.12%), Postives = 118/168 (70.24%), Query Frame = 0


Query: 28  KKKKKENELEKEETGCWVRLKFIRSCIPSRSKVDSSLSGSKVDSFISGTTTHCDSKSKLD 87
           KKK  + + ++ E+G W R KFI SCI SRSKVDSS++ + V                + 
Sbjct: 4   KKKNVKKDGDESESGFWFRFKFIFSCISSRSKVDSSMNATAV----------------IA 63

Query: 88  EKKVILFTALGSSATTDN---AESSSSTPKLSAELKVASRLRKFTFNELKLATRNFRPES 147
           E K ++    G  A T +   AES SSTP +S ELK +S+LR F FN+LKLATRNFRPES
Sbjct: 64  EPKKVIEKLEGHPAPTKDTGCAESGSSTPLMSGELKYSSKLRIFMFNDLKLATRNFRPES 123

Query: 148 LLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTLNHDGLQGHKEWM 193
           LLGEGGFGCVFKGWIEENGTAP KPGTGLTVAVKTLN DGLQGHKEW+
Sbjct: 124 LLGEGGFGCVFKGWIEENGTAPVKPGTGLTVAVKTLNPDGLQGHKEWL 155

[Close]

BLAST of Carg04394 vs. TAIR 10
Match: AT3G28690.3 (Protein kinase superfamily protein )

HSP 1 Score: 157.1 bits (396), Expect = 1.4e-38
Identity = 81/110 (73.64%), Postives = 88/110 (80.00%), Query Frame = 0


Query: 85  KLDEKKVILFTALGSSATTDN--AESSSSTPKLSAELKVASRLRKFTFNELKLATRNFRP 144
           K + KKVI       + T D   AES SSTP +S ELK +S+LR F FN+LKLATRNFRP
Sbjct: 18  KAEPKKVIEKLEGHPAPTKDTGCAESGSSTPLMSGELKYSSKLRIFMFNDLKLATRNFRP 77

Query: 145 ESLLGEGGFGCVFKGWIEENGTAPAKPGTGLTVAVKTLNHDGLQGHKEWM 193
           ESLLGEGGFGCVFKGWIEENGTAP KPGTGLTVAVKTLN DGLQGHKEW+
Sbjct: 78  ESLLGEGGFGCVFKGWIEENGTAPVKPGTGLTVAVKTLNPDGLQGHKEWL 127

[Close]

BLAST of Carg04394 vs. TAIR 10
Match: AT3G28690.1 (Protein kinase superfamily protein )

HSP 1 Score: 145.6 bits (366), Expect = 4.2e-35
Identity = 67/78 (85.90%), Postives = 72/78 (92.31%), Query Frame = 0


Query: 115 LSAELKVASRLRKFTFNELKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPAKPGTGLT 174
           +S ELK +S+LR F FN+LKLATRNFRPESLLGEGGFGCVFKGWIEENGTAP KPGTGLT
Sbjct: 1   MSGELKYSSKLRIFMFNDLKLATRNFRPESLLGEGGFGCVFKGWIEENGTAPVKPGTGLT 60

Query: 175 VAVKTLNHDGLQGHKEWM 193
           VAVKTLN DGLQGHKEW+
Sbjct: 61  VAVKTLNPDGLQGHKEWL 78

The following BLAST results are available for this feature:

BLAST of Carg04394 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7033158.1	9.1e-101	100.00	putative serine/threonine-protein kinase PIX7, partial [Cucurbita argyrosperma s...	[more]
XP_022962548.1	2.7e-100	100.00	probable serine/threonine-protein kinase PIX7 isoform X1 [Cucurbita moschata]	[more]
XP_022962554.1	2.7e-100	100.00	probable serine/threonine-protein kinase PIX7 isoform X2 [Cucurbita moschata]	[more]
KAG6602482.1	1.5e-98	99.48	putative serine/threonine-protein kinase PIX7, partial [Cucurbita argyrosperma s...	[more]
XP_022991185.1	7.2e-98	98.96	probable serine/threonine-protein kinase PIX7 isoform X1 [Cucurbita maxima]	[more]

BLAST of Carg04394 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LFP7	3.8e-49	56.50	Serine/threonine-protein kinase PBL34 OS=Arabidopsis thaliana OX=3702 GN=PBL34 P...	[more]
Q9SRH7	5.1e-46	54.21	Serine/threonine-protein kinase PBL35 OS=Arabidopsis thaliana OX=3702 GN=PBL35 P...	[more]
F4J0D2	1.3e-44	57.22	Serine/threonine-protein kinase PBL36 OS=Arabidopsis thaliana OX=3702 GN=PBL36 P...	[more]
O48814	1.3e-25	53.19	Serine/threonine-protein kinase BIK1 OS=Arabidopsis thaliana OX=3702 GN=BIK1 PE=...	[more]
P43293	8.5e-25	57.65	Probable serine/threonine-protein kinase PBL11 OS=Arabidopsis thaliana OX=3702 G...	[more]

BLAST of Carg04394 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HF39	1.3e-100	100.00	probable serine/threonine-protein kinase PIX7 isoform X1 OS=Cucurbita moschata O...	[more]
A0A6J1HHF4	1.3e-100	100.00	probable serine/threonine-protein kinase PIX7 isoform X2 OS=Cucurbita moschata O...	[more]
A0A6J1JS65	3.5e-98	98.96	probable serine/threonine-protein kinase PIX7 isoform X1 OS=Cucurbita maxima OX=...	[more]
A0A6J1JQ16	3.5e-98	98.96	probable serine/threonine-protein kinase PIX7 isoform X2 OS=Cucurbita maxima OX=...	[more]
A0A0A0KXB4	5.4e-91	93.75	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G635...	[more]

BLAST of Carg04394 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G15080.1	2.7e-50	56.50	Protein kinase superfamily protein	[more]
AT3G01300.1	3.6e-47	54.21	Protein kinase superfamily protein	[more]
AT3G28690.2	1.4e-46	60.12	Protein kinase superfamily protein	[more]
AT3G28690.3	1.4e-38	73.64	Protein kinase superfamily protein	[more]
AT3G28690.1	4.2e-35	85.90	Protein kinase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 19..42
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 124..193 e-value: 4.5E-14 score: 54.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..39
None	No IPR available	PANTHER	PTHR45621:SF182	KINASE APK1B, CHLOROPLAST, PUTATIVE-RELATED	coord: 66..192
None	No IPR available	PANTHER	PTHR45621	OS01G0588500 PROTEIN-RELATED	coord: 66..192
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 146..178
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 113..189

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg04394-RA	Carg04394-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004674	protein serine/threonine kinase activity