Carg04339 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg04339
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionVacuolar cation/proton exchanger
LocationCarg_Chr01: 106379 .. 110627 (-)
RNA-Seq ExpressionCarg04339
SyntenyCarg04339
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTATTCTGTTTTATTATTGTTAATTTTATAAAAAATTACACTTAATAAACAAGTCAAAGCTAAGCGGGGAAGACGCAAGAGGACGATATTTCGCAGAAGCTTCCTAGCTGCTGTGGAGCTTGCCGTCTTGCCTTCCCTTCCTAATCGCCACCCACTCTCGTCTTCTTCATCGCTTGGTCTATTTATTATTTTTGTGAACTCGAGGATCTTATTTCGAAGATCTTTCCTTCTGTCTGGCCTTTGCCGACTGTGGTCACCCAAGTATTTATTTTTAAGCAACCGAATGAACCTATACGATCCAATCTAGTGGGACCCGATTGTTTCTTGTCTTACTCTCGCCTTCTTCCTTTTGAGGTTGGTATCTCAGCTCCATTTCAGGAGTTCTTAATCATTTTGCAGCTGGGATTCGACCTGGATATCCTCGTTGCGTCACTTCAAGTTCATCTCCAGCCTGGTAAATGCTCCCCCTCATTCTTTCTATTCATGCACTCACTAAACCTCGTCCTAATCTCGACTCGATCTGGTTTCTTCTTTCTGTTAAGTTAAAAATGCTTCGGAGTTGACATGTGGAAGATGAAGTAGCTGATGGGTAGCACATGTAAATCTACCCAGATTTGTATTTTACTGTAGTTTTGTCTAAATGGGTCTTTGGTATTGGAAGTGAAAAGGGTTAATTGATCGAGATAACTATGCTTGCAGATGGGATCGATAGAGGAGGGAACAAATTACCTATCTGACGAAGATATTCCATTGAAGGGAACACCAGTTGCGAAAAACTTATCGCATAGCTTTGACTGTGAGACGGTTCATGTGCGTTCCTCCCCAGATGATAAAAGATGGTGGAAGACTAACAACATTCGTTTTAGACCTCTTGAAAGTGCTAAAATAGTGTTGATTAAAGCAAAGATCAACGTGTTACTTCCTTTTGGTCCTTTGGCAATCCTGCTACATTATTTAACTGGAAAGCATGTAAGAAGTAGTACTCCTCTTCATGATCTTACTGGTTGCATTCTGAAATTTGTATTAAGCAAATGACATGACTAGATTTGAGAACAAAGAAATGGTGGAAGACTTGTATTTAGCAAGCCTTTCATGAAACAGAAGCTCCTAGACAATCAAATATTTTTCCTAATTATTTGATTTGATGAGGATATGGTCTATGATGACCTCGTTGTGATGCTTTATATCATCTATTGATGTACTCATAAAATCTTGGATCTTCGAACATTCTAACTTTAGGATCTTTTTGCTGCTTAGTACCGTGGCTTGTATGATCTGTCTCATGGTTCATAATTCCTTGATAGTCAGCTTTATATGTCAGGGTTTGGTCTTCTTTTTCACGTTACTTGGAATTACACCTTTGGCAGAACGTCTTGGTTATGCTACAGAGTAAGATTTGGCCTCCCATATTCTCCGTTTAGGATTTCATATTATTTCTATTGTGCTACACTAACTTTTAAAAATACTCGTCATGCAGGCAGCTTGCTTCCTATACTGGTCCAACAGGTACAATTGCTAGCATGATCTGGCATTGCTTGTTTTGGTCATTTTCTTTATTCAAGTAGTTCCTCGTTTGATCTCTAATCGATGGCTGTTCAGTTAGTTTCTCTAGTAACTCAAATGTTTTAATGGACTTTGCAGTTGGTGGTCTTTTGAATGCAACATTTGGAAATGCAACCGAAATGATTATATCGATTTATGCATTAAAGAGTGGGATGATTAGGGTTGTTCAACAGTCGTTATTGGGATCAATCTTGTCGAATATGCTCCTAGTGCTTGGTTGCGCTTTCTTCACTGGTGGGATTGTTCATCATAAGAATGTGCAGACTTTCAATAAGGTACAAAGTTTTGAGCTCTATGAAATGTTCTTCACCGGTGGTGATATTTTACTCCATTTTCTTGGTTTACATTTAAATCTGACTATTTGGCAACAGGGTACTGCTGTTGTGAATTCAGGATTGTTGTTAATGGCTGTTATGGGAACAACGTTTCCGGCTGTGCTTCATTTCACTCACACAGAACTTCATTCTGGAGAGTCAGCATTGTCTCTTTCAAGGTTTAGCAGTTGTATAATGTTAATTGCATATGCAAGCTTCCTTTTCTTTCAACTAAAAAGTCAGCACAATCTCTATGGTTCTCTTGATGAGGTGGGTCTAAAAAATGCAGAATCAAGGAAATAGCTATCTATCTTTTCCACGCAGTAGTTTCATTTCAAGCAGAGATGCTTTTAATTCTAATGGTTTTAGCCTTTTTATATTTCTTTAGGAAGGTGATGGTGAAGTTGAGGAGGATCCTGAGATTTATGCTTGGGAAGCAATTGGGTGGCTTGCTATCTTGACTGTATGGGTATCGATTTTATCTGGATACCTTGTTGATGCTATCGAGGTAAACTTTATTCTAATCAGCATTACAACTTTATTCTAATCCAGGGCTCGACTCTAATGTTTATTCATGGTAATTCAAAGCTTTTCTGTAGTGAAGCTTACAAACTTTGTTCAGTAGGTTTCTTTTCTGGAAACCCTTGATAAAATAACAGCAAGAAGATATAGAAGAACAAAAACTATTTTTCACTCACCTAAAATGTTGATGTTGTGTGAATCTCTTAACGTCAAAATTCTACGGTTGAACAGGGAAAGTTTCTAGTACTACCACAGTACAAACTCTTTTCATTTAGCATTTGACACGTCATTTAGTGATATATATATATATACACACACATGCTAAGCTTCTAGGCTTCGCACCATTCTTAGATATTTCTTTTATGACAAGTATTGAAGAGAAGTAGTTTGAAATTTTTTGTGTACTGCTATTGCACTATAATTTTTTCTGATTAAAACAGTAATCCTTTGGGACAAACTCATGTTCTATGGTCTGTTCTCATTTGCCTTCGGATCACGCATATTTTCTATATGAAGTCTTCAAATTCTGCACTTCATTTTTATCTTGGTCGCTGGACTCTCTAATTTTTTCCCTAAAAAAAAAACCAAAAAAAAAAAAAAACAAAAAAAAAAAGTGACAAGGACCTTGCAGTATCCGGCAAATTTAATTAGTTGGCGTGTATTCTCTCAGGGAGCATCTGAATCATTACATTTGCCAGTGGCTTTTATAAGTGTAATTTTGTTGCCAATAGTTGGAAATGCTGCTGAACATGCAAGTGCAATCATGTTTGCCATGAAGGATAAGCTTGTGAGTACTTTGTGGATTTTTTTTCATTTTCATCTAATCATCTATTTTCATTGGCTTAATTGACCTAAAAGTTACTGCAGAACATCACCCTTGGAGTTGCTATTGGATCATCAACACAAATATCCATGTTTGTGGTTAGTCTTGAAGATTATTTTTAAGTTCCTAATAACACTTAAAATCATTTGATTCTAACAAAACTTTAGGTGAAATGGAAACAGAATTACCACTAGTTCAACTCTTCTATAACATTTAGCTGTACTGTGTGCCATTGACGATTGCAGATCCCATTTTGCGTCGTTGTTGGATGGTGTATGGGAATACCAATGGACTTGAACTTTAAACTCTTTGAGACTGCAACTCTGTTTATAACTGTGCTAGTGGTGGCCTTTATGTTGCAGGTGCGTTATCTTCTATCCATCCTTCCATATTTAACGCTGCATTATGGGACTATTACTTTGAACTTTTCGGTGCATTTTCATTAAAAAGTTTCGTTTATATACCAAAAGCCATTCCGTCGGAACATGAATGTATAAACGAAAATGGGAAATAAAAGTGGCTTATCCATGATAAGTTCAGTATAAATGCATTAGACACTGTGGTATGCGAACAATTGCTGTCCTTATCTCTGTTTGTTCTGTACGATAAGTCATTGTTCTCATGTTCTTGGCAATGGTCATATTTGCAGGAGGGGACGTCAAATTATTTCAAAGGTCTAATGCTTATTCTATGCTACCTCATTGTGGCAGCCAGCTTCTTTGTACATGTTGACCCTAGCAGTGGTGAGTAGTTACTCCTTCAATCCACTACAAATAGAAAATCTACTGCTTGGTTTCTGGAATTGCCTTGTAATTACATCTTCTCACCTTATTTATTTTTAGTTTAACGGTATGTTGGGGTTGGAGATTCGAACCTCTGACCTTTTGGCTGAAGTTACATGTTTTATGCTAGTTGAGCTTTGATTTCTCTGAAATATTTTTGTTCATTATAATCTTCTAGACTTGTGGACATAGGATTCCCTTCTTATGTCGATATTTAACAGCTAAAACTGCGACTGAATTTGGCT

mRNA sequence

TTATTCTGTTTTATTATTGTTAATTTTATAAAAAATTACACTTAATAAACAAGTCAAAGCTAAGCGGGGAAGACGCAAGAGGACGATATTTCGCAGAAGCTTCCTAGCTGCTGTGGAGCTTGCCGTCTTGCCTTCCCTTCCTAATCGCCACCCACTCTCGTCTTCTTCATCGCTTGGTCTATTTATTATTTTTGTGAACTCGAGGATCTTATTTCGAAGATCTTTCCTTCTGTCTGGCCTTTGCCGACTGTGGTCACCCAAGTATTTATTTTTAAGCAACCGAATGAACCTATACGATCCAATCTAGTGGGACCCGATTGTTTCTTGTCTTACTCTCGCCTTCTTCCTTTTGAGGTTGGTATCTCAGCTCCATTTCAGGAGTTCTTAATCATTTTGCAGCTGGGATTCGACCTGGATATCCTCGTTGCGTCACTTCAAGTTCATCTCCAGCCTGGTAAATGCTCCCCCTCATTCTTTCTATTCATGCACTCACTAAACCTCGTCCTAATCTCGACTCGATCTGGTTTCTTCTTTCTGTTAAGTTAAAAATGCTTCGGAGTTGACATGTGGAAGATGAAGTAGCTGATGGGTAGCACATGTAAATCTACCCAGGGTTAATTGATCGAGATAACTATGCTTGCAGATGGGATCGATAGAGGAGGGAACAAATTACCTATCTGACGAAGATATTCCATTGAAGGGAACACCAGTTGCGAAAAACTTATCGCATAGCTTTGACTGTGAGACGGTTCATGTGCGTTCCTCCCCAGATGATAAAAGATGGTGGAAGACTAACAACATTCGTTTTAGACCTCTTGAAAGTGCTAAAATAGTGTTGATTAAAGCAAAGATCAACGTGTTACTTCCTTTTGGTCCTTTGGCAATCCTGCTACATTATTTAACTGGAAAGCATGGTTTGGTCTTCTTTTTCACGTTACTTGGAATTACACCTTTGGCAGAACGTCTTGGTTATGCTACAGAGCAGCTTGCTTCCTATACTGGTCCAACAGTTGGTGGTCTTTTGAATGCAACATTTGGAAATGCAACCGAAATGATTATATCGATTTATGCATTAAAGAGTGGGATGATTAGGGTTGTTCAACAGTCGTTATTGGGATCAATCTTGTCGAATATGCTCCTAGTGCTTGGTTGCGCTTTCTTCACTGGTGGGATTGTTCATCATAAGAATGTGCAGACTTTCAATAAGGGTACTGCTGTTGTGAATTCAGGATTGTTGTTAATGGCTGTTATGGGAACAACGTTTCCGGCTGTGCTTCATTTCACTCACACAGAACTTCATTCTGGAGAGTCAGCATTGTCTCTTTCAAGGTTTAGCAGTTGTATAATGTTAATTGCATATGCAAGCTTCCTTTTCTTTCAACTAAAAAGTCAGCACAATCTCTATGGTTCTCTTGATGAGGAAGGTGATGGTGAAGTTGAGGAGGATCCTGAGATTTATGCTTGGGAAGCAATTGGGTGGCTTGCTATCTTGACTGTATGGGTATCGATTTTATCTGGATACCTTGTTGATGCTATCGAGGGAGCATCTGAATCATTACATTTGCCAGTGGCTTTTATAAGTGTAATTTTGTTGCCAATAGTTGGAAATGCTGCTGAACATGCAAGTGCAATCATGTTTGCCATGAAGGATAAGCTTAACATCACCCTTGGAGTTGCTATTGGATCATCAACACAAATATCCATGTTTGTGATCCCATTTTGCGTCGTTGTTGGATGGTGTATGGGAATACCAATGGACTTGAACTTTAAACTCTTTGAGACTGCAACTCTGTTTATAACTGTGCTAGTGGTGGCCTTTATGTTGCAGGAGGGGACGTCAAATTATTTCAAAGGTCTAATGCTTATTCTATGCTACCTCATTGTGGCAGCCAGCTTCTTTGTACATGTTGACCCTAGCAGTGGTGAGTAGTTACTCCTTCAATCCACTACAAATAGAAAATCTACTGCTTGGTTTCTGGAATTGCCTTGTAATTACATCTTCTCACCTTATTTATTTTTAGTTTAACGGTATGTTGGGGTTGGAGATTCGAACCTCTGACCTTTTGGCTGAAGTTACATGTTTTATGCTAGTTGAGCTTTGATTTCTCTGAAATATTTTTGTTCATTATAATCTTCTAGACTTGTGGACATAGGATTCCCTTCTTATGTCGATATTTAACAGCTAAAACTGCGACTGAATTTGGCT

Coding sequence (CDS)

ATGGGATCGATAGAGGAGGGAACAAATTACCTATCTGACGAAGATATTCCATTGAAGGGAACACCAGTTGCGAAAAACTTATCGCATAGCTTTGACTGTGAGACGGTTCATGTGCGTTCCTCCCCAGATGATAAAAGATGGTGGAAGACTAACAACATTCGTTTTAGACCTCTTGAAAGTGCTAAAATAGTGTTGATTAAAGCAAAGATCAACGTGTTACTTCCTTTTGGTCCTTTGGCAATCCTGCTACATTATTTAACTGGAAAGCATGGTTTGGTCTTCTTTTTCACGTTACTTGGAATTACACCTTTGGCAGAACGTCTTGGTTATGCTACAGAGCAGCTTGCTTCCTATACTGGTCCAACAGTTGGTGGTCTTTTGAATGCAACATTTGGAAATGCAACCGAAATGATTATATCGATTTATGCATTAAAGAGTGGGATGATTAGGGTTGTTCAACAGTCGTTATTGGGATCAATCTTGTCGAATATGCTCCTAGTGCTTGGTTGCGCTTTCTTCACTGGTGGGATTGTTCATCATAAGAATGTGCAGACTTTCAATAAGGGTACTGCTGTTGTGAATTCAGGATTGTTGTTAATGGCTGTTATGGGAACAACGTTTCCGGCTGTGCTTCATTTCACTCACACAGAACTTCATTCTGGAGAGTCAGCATTGTCTCTTTCAAGGTTTAGCAGTTGTATAATGTTAATTGCATATGCAAGCTTCCTTTTCTTTCAACTAAAAAGTCAGCACAATCTCTATGGTTCTCTTGATGAGGAAGGTGATGGTGAAGTTGAGGAGGATCCTGAGATTTATGCTTGGGAAGCAATTGGGTGGCTTGCTATCTTGACTGTATGGGTATCGATTTTATCTGGATACCTTGTTGATGCTATCGAGGGAGCATCTGAATCATTACATTTGCCAGTGGCTTTTATAAGTGTAATTTTGTTGCCAATAGTTGGAAATGCTGCTGAACATGCAAGTGCAATCATGTTTGCCATGAAGGATAAGCTTAACATCACCCTTGGAGTTGCTATTGGATCATCAACACAAATATCCATGTTTGTGATCCCATTTTGCGTCGTTGTTGGATGGTGTATGGGAATACCAATGGACTTGAACTTTAAACTCTTTGAGACTGCAACTCTGTTTATAACTGTGCTAGTGGTGGCCTTTATGTTGCAGGAGGGGACGTCAAATTATTTCAAAGGTCTAATGCTTATTCTATGCTACCTCATTGTGGCAGCCAGCTTCTTTGTACATGTTGACCCTAGCAGTGGTGAGTAG

Protein sequence

MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLESAKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHHKNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYASFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEGASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFCVVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFVHVDPSSGE
Homology
BLAST of Carg04339 vs. NCBI nr
Match: KAG7036402.1 (Vacuolar cation/proton exchanger 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 834.7 bits (2155), Expect = 3.5e-238
Identity = 428/428 (100.00%), Postives = 428/428 (100.00%), Query Frame = 0

Query: 1   MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLES 60
           MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLES
Sbjct: 1   MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLES 60

Query: 61  AKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTG 120
           AKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTG
Sbjct: 61  AKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180

Query: 181 KNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240
           KNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA
Sbjct: 181 KNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240

Query: 241 SFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEG 300
           SFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEG
Sbjct: 241 SFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEG 300

Query: 301 ASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFC 360
           ASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFC
Sbjct: 301 ASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFC 360

Query: 361 VVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV
Sbjct: 361 VVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420

Query: 421 HVDPSSGE 429
           HVDPSSGE
Sbjct: 421 HVDPSSGE 428

BLAST of Carg04339 vs. NCBI nr
Match: XP_022949566.1 (vacuolar cation/proton exchanger 3-like [Cucurbita moschata] >XP_022949567.1 vacuolar cation/proton exchanger 3-like [Cucurbita moschata] >XP_023521834.1 vacuolar cation/proton exchanger 3-like [Cucurbita pepo subsp. pepo] >XP_023521840.1 vacuolar cation/proton exchanger 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 830.9 bits (2145), Expect = 5.1e-237
Identity = 426/428 (99.53%), Postives = 427/428 (99.77%), Query Frame = 0

Query: 1   MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLES 60
           MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLES
Sbjct: 1   MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLES 60

Query: 61  AKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTG 120
           AKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTG
Sbjct: 61  AKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180

Query: 181 KNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240
           KNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA
Sbjct: 181 KNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240

Query: 241 SFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEG 300
           SFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEG
Sbjct: 241 SFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEG 300

Query: 301 ASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFC 360
           ASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFC
Sbjct: 301 ASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFC 360

Query: 361 VVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV
Sbjct: 361 VVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420

Query: 421 HVDPSSGE 429
           HVDPSS +
Sbjct: 421 HVDPSSDD 428

BLAST of Carg04339 vs. NCBI nr
Match: KAG6606682.1 (Vacuolar cation/proton exchanger 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 830.9 bits (2145), Expect = 5.1e-237
Identity = 426/428 (99.53%), Postives = 427/428 (99.77%), Query Frame = 0

Query: 1   MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLES 60
           MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLES
Sbjct: 69  MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLES 128

Query: 61  AKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTG 120
           AKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTG
Sbjct: 129 AKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTG 188

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH
Sbjct: 189 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 248

Query: 181 KNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240
           KNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA
Sbjct: 249 KNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 308

Query: 241 SFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEG 300
           SFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEG
Sbjct: 309 SFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEG 368

Query: 301 ASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFC 360
           ASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFC
Sbjct: 369 ASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFC 428

Query: 361 VVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV
Sbjct: 429 VVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 488

Query: 421 HVDPSSGE 429
           HVDPSS +
Sbjct: 489 HVDPSSDD 496

BLAST of Carg04339 vs. NCBI nr
Match: XP_022998783.1 (vacuolar cation/proton exchanger 3-like [Cucurbita maxima] >XP_022998784.1 vacuolar cation/proton exchanger 3-like [Cucurbita maxima])

HSP 1 Score: 829.7 bits (2142), Expect = 1.1e-236
Identity = 425/428 (99.30%), Postives = 427/428 (99.77%), Query Frame = 0

Query: 1   MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLES 60
           MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLES
Sbjct: 1   MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLES 60

Query: 61  AKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTG 120
           AKIVLIKAKIN+LLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTG
Sbjct: 61  AKIVLIKAKINLLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180

Query: 181 KNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240
           KNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA
Sbjct: 181 KNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240

Query: 241 SFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEG 300
           SFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEG
Sbjct: 241 SFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEG 300

Query: 301 ASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFC 360
           ASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFC
Sbjct: 301 ASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFC 360

Query: 361 VVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV
Sbjct: 361 VVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420

Query: 421 HVDPSSGE 429
           HVDPSS +
Sbjct: 421 HVDPSSDD 428

BLAST of Carg04339 vs. NCBI nr
Match: XP_008463979.1 (PREDICTED: vacuolar cation/proton exchanger 3-like [Cucumis melo] >XP_008463980.1 PREDICTED: vacuolar cation/proton exchanger 3-like [Cucumis melo] >XP_008463981.1 PREDICTED: vacuolar cation/proton exchanger 3-like [Cucumis melo])

HSP 1 Score: 760.8 bits (1963), Expect = 6.5e-216
Identity = 393/428 (91.82%), Postives = 407/428 (95.09%), Query Frame = 0

Query: 1   MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLES 60
           MGS EE TNYLSDE+IPLK +PVAKN+ HSFDCET  VRSSP D RWWKTNNIR RPL S
Sbjct: 1   MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSP-DTRWWKTNNIRIRPLRS 60

Query: 61  AKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTG 120
           A IVL++AKINVLLPFGPLAILLHYLTGKHG VFFFTLLGITPLAERLGYATEQLA YTG
Sbjct: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI+HH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180

Query: 181 KNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240
           K VQ FNKG AVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA
Sbjct: 181 KKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240

Query: 241 SFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEG 300
           S+LFFQLKSQHNLYG LDEE DGEVE+D EI+AWEAIGWLAILTVWVSILSGYLVDAI+G
Sbjct: 241 SYLFFQLKSQHNLYGPLDEEVDGEVEDDSEIFAWEAIGWLAILTVWVSILSGYLVDAIQG 300

Query: 301 ASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFC 360
           ASESL+LPVAFISVILLPIVGNAAEHASAIMFAMKDKL+ITLGVAIGSSTQISMFVIPFC
Sbjct: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360

Query: 361 VVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMG PMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV
Sbjct: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420

Query: 421 HVDPSSGE 429
           HVDPS+ +
Sbjct: 421 HVDPSNDD 427

BLAST of Carg04339 vs. ExPASy Swiss-Prot
Match: Q5KQN0 (Vacuolar cation/proton exchanger 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CAX2 PE=2 SV=2)

HSP 1 Score: 569.7 bits (1467), Expect = 2.8e-161
Identity = 310/427 (72.60%), Postives = 349/427 (81.73%), Query Frame = 0

Query: 4   IEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLESAKI 63
           +EEG    S   +   G   A +  H      V    S   K  W+      R L S ++
Sbjct: 15  LEEGGGSPSPSPMTAAGKMQALDFEHIGSLAAVAESLSTGSK--WR------RALTSVRV 74

Query: 64  VLIKAKINVLLPFGPLAILLHYLTGKH-GLVFFFTLLGITPLAERLGYATEQLASYTGPT 123
           V+++AKINVLLPFGPLA++LHYL+  H G VF F+L+GITPLAERLGYATEQLA YTGPT
Sbjct: 75  VILQAKINVLLPFGPLAVMLHYLSANHQGWVFLFSLIGITPLAERLGYATEQLALYTGPT 134

Query: 124 VGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHHKN 183
           +GGLLNATFGNATEMIIS+YALK+GMIRVVQQSLLGSILSNMLLVLGCAFF GG+VH   
Sbjct: 135 IGGLLNATFGNATEMIISLYALKNGMIRVVQQSLLGSILSNMLLVLGCAFFAGGLVHPSR 194

Query: 184 VQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYASF 243
            Q FNK +AVVNSGLLLMAV+G  FPAVLHFTH+E+  G+S +SLSRFSSCIML+AYAS+
Sbjct: 195 DQVFNKASAVVNSGLLLMAVLGLMFPAVLHFTHSEVQYGKSEVSLSRFSSCIMLVAYASY 254

Query: 244 LFFQLKSQHNLY---GSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIE 303
           LFFQLKSQ +LY   G  +EE   + EE+ EI   EAI WL +LT+W+SILSGYLVDAI+
Sbjct: 255 LFFQLKSQRSLYSPIGEQEEEVTEDEEEEKEITQGEAICWLFVLTIWISILSGYLVDAIQ 314

Query: 304 GASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPF 363
           GASESL++PVAFISVILLPIVGNAAEHASAIMFAMKDKL+ITLGVAIGSSTQISMFVIPF
Sbjct: 315 GASESLNMPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPF 374

Query: 364 CVVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFF 423
           CVV+GW MG  MDLNF+LFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFF
Sbjct: 375 CVVIGWIMGQQMDLNFQLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFF 433

Query: 424 VHVDPSS 427
           VHVDP S
Sbjct: 435 VHVDPDS 433

BLAST of Carg04339 vs. ExPASy Swiss-Prot
Match: Q6K1C4 (Vacuolar cation/proton exchanger 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CAX3 PE=2 SV=2)

HSP 1 Score: 556.2 bits (1432), Expect = 3.2e-157
Identity = 290/386 (75.13%), Postives = 327/386 (84.72%), Query Frame = 0

Query: 48  WKTNNIRFRPLESAKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAER 107
           W         L S +IV+  +K+N+LLPFGP +I+LHY T +HGLVF F++LGITPLAER
Sbjct: 31  WNLQRFVESALRSIRIVIFTSKLNLLLPFGPASIILHYTTSRHGLVFLFSMLGITPLAER 90

Query: 108 LGYATEQLASYTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLV 167
           LGYATEQLA YTGPTVGGLLNATFGNATEMII+IYALK+GMIRVVQQSLLGSILSNMLLV
Sbjct: 91  LGYATEQLAIYTGPTVGGLLNATFGNATEMIIAIYALKNGMIRVVQQSLLGSILSNMLLV 150

Query: 168 LGCAFFTGGIVHHKNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSL 227
           +GCAFF GGIVH    Q F+K TAVVNSGLLLMAVMG  FPAVLHFTH+E+  G S +SL
Sbjct: 151 MGCAFFAGGIVHRNKDQVFSKATAVVNSGLLLMAVMGLMFPAVLHFTHSEVRQGASEVSL 210

Query: 228 SRFSSCIMLIAYASFLFFQLKSQHNLYG-----SLDEEGDGEVEEDPEIYAWEAIGWLAI 287
           SRFSSCIML+AYAS+L+FQL  ++N Y       +  E   E +E+ EI  WE+I WLA+
Sbjct: 211 SRFSSCIMLVAYASYLYFQLSGRNNAYSPIGSEEMPNEDAAEEDEESEIGMWESIAWLAM 270

Query: 288 LTVWVSILSGYLVDAIEGASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITL 347
           LT+WVSILS YLV+AIEGAS+SL+LPVAFISVILLPIVGNAAEHASAIMFAMKDKL+ITL
Sbjct: 271 LTLWVSILSEYLVNAIEGASDSLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITL 330

Query: 348 GVAIGSSTQISMFVIPFCVVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYF 407
           GVAIGSSTQISMFVIPFCVV+GW MG  MDLNF+LFETATLFITVLVVAFMLQ+G +NY 
Sbjct: 331 GVAIGSSTQISMFVIPFCVVIGWMMGQKMDLNFQLFETATLFITVLVVAFMLQDGVANYL 390

Query: 408 KGLMLILCYLIVAASFFVHVDPSSGE 429
           KGLMLILCYLIVAASFFVHVDP S +
Sbjct: 391 KGLMLILCYLIVAASFFVHVDPQSSD 416

BLAST of Carg04339 vs. ExPASy Swiss-Prot
Match: Q8L783 (Vacuolar cation/proton exchanger 5 OS=Arabidopsis thaliana OX=3702 GN=CAX5 PE=2 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 6.9e-152
Identity = 282/372 (75.81%), Postives = 320/372 (86.02%), Query Frame = 0

Query: 58  LESAKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLAS 117
           L+S KIV++  K+N+LLPFGPLAILLHYLT   G +F  +L+GITPLAERLGYATEQLA 
Sbjct: 65  LQSFKIVILSNKLNLLLPFGPLAILLHYLTDNKGWIFLLSLVGITPLAERLGYATEQLAC 124

Query: 118 YTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI 177
           YTG TVGGLLNATFGN TE+IISI+ALKSGMIRVVQ +LLGSILSNMLLVLGCAFF GG+
Sbjct: 125 YTGSTVGGLLNATFGNVTELIISIFALKSGMIRVVQLTLLGSILSNMLLVLGCAFFCGGL 184

Query: 178 VHHKNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLI 237
           V  +  Q F+KG AVVNSGLLLMAVMG  FPAVLH+TH+E+H+G S L+LSRFSSCIML+
Sbjct: 185 VFSQKEQVFDKGNAVVNSGLLLMAVMGLLFPAVLHYTHSEVHAGSSELALSRFSSCIMLV 244

Query: 238 AYASFLFFQLKSQHNLYGSLDEEGD-----GEVEEDPEIYAWEAIGWLAILTVWVSILSG 297
           AYA++LFFQLKSQ + Y  L EE +      + +EDPEI  WEAI WL+ILT WVS+LSG
Sbjct: 245 AYAAYLFFQLKSQPSSYTPLTEETNQNEETSDDDEDPEISKWEAIIWLSILTAWVSLLSG 304

Query: 298 YLVDAIEGASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQI 357
           YLVDAIEGAS S  +P++FISVILLPIVGNAAEHA AIMFAMKDKL+++LGVAIGSS QI
Sbjct: 305 YLVDAIEGASVSWKIPISFISVILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSSIQI 364

Query: 358 SMFVIPFCVVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYL 417
           SMF +PFCVV+GW MG  MDLNF+LFETATLFITV+VVAF LQEGTSNYFKGLMLILCYL
Sbjct: 365 SMFAVPFCVVIGWMMGAQMDLNFQLFETATLFITVIVVAFFLQEGTSNYFKGLMLILCYL 424

Query: 418 IVAASFFVHVDP 425
           IVAASFFVH DP
Sbjct: 425 IVAASFFVHEDP 436

BLAST of Carg04339 vs. ExPASy Swiss-Prot
Match: Q39254 (Vacuolar cation/proton exchanger 2 OS=Arabidopsis thaliana OX=3702 GN=CAX2 PE=1 SV=2)

HSP 1 Score: 537.3 bits (1383), Expect = 1.5e-151
Identity = 282/372 (75.81%), Postives = 317/372 (85.22%), Query Frame = 0

Query: 58  LESAKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLAS 117
           L S KIV+   K+N+LLPFGPLAIL+HY+    G VF  TL+GITPLAERLGYATEQLA 
Sbjct: 65  LNSIKIVIFCNKLNLLLPFGPLAILVHYMIDSKGWVFLLTLVGITPLAERLGYATEQLAC 124

Query: 118 YTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI 177
           YTGPTVGGLLNATFGN TE+IISI+ALK+GMIRVVQ +LLGSILSNMLLVLGCAFF GG+
Sbjct: 125 YTGPTVGGLLNATFGNVTELIISIFALKNGMIRVVQLTLLGSILSNMLLVLGCAFFCGGL 184

Query: 178 VHHKNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLI 237
           V ++  Q F+KG A VNSGLLLMAVMG  FPAVLH+TH+E+H+G S L+LSRFSSCIMLI
Sbjct: 185 VFYQKDQVFDKGIATVNSGLLLMAVMGILFPAVLHYTHSEVHAGSSELALSRFSSCIMLI 244

Query: 238 AYASFLFFQLKSQHNLYGSLDEEGDGEVE-----EDPEIYAWEAIGWLAILTVWVSILSG 297
           AYA++LFFQLKSQ N Y  LDEE +   E     EDPEI  WEAI WL+ILT WVS+LSG
Sbjct: 245 AYAAYLFFQLKSQSNSYSPLDEESNQNEETSAEDEDPEISKWEAIIWLSILTAWVSLLSG 304

Query: 298 YLVDAIEGASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQI 357
           YLVDAIEGAS S ++P+AFIS ILLPIVGNAAEHA AIMFAMKDKL+++LGVAIGSS QI
Sbjct: 305 YLVDAIEGASVSWNIPIAFISTILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSSIQI 364

Query: 358 SMFVIPFCVVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYL 417
           SMF +PFCVV+GW MG  MDLNF+LFETA LFITV+VVAF LQEG+SNYFKGLMLILCYL
Sbjct: 365 SMFAVPFCVVIGWMMGQQMDLNFQLFETAMLFITVIVVAFFLQEGSSNYFKGLMLILCYL 424

Query: 418 IVAASFFVHVDP 425
           IVAASFFVH DP
Sbjct: 425 IVAASFFVHEDP 436

BLAST of Carg04339 vs. ExPASy Swiss-Prot
Match: Q9LFZ8 (Putative vacuolar cation/proton exchanger 6 OS=Arabidopsis thaliana OX=3702 GN=CAX6 PE=3 SV=3)

HSP 1 Score: 498.0 bits (1281), Expect = 1.0e-139
Identity = 263/373 (70.51%), Postives = 305/373 (81.77%), Query Frame = 0

Query: 58  LESAKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLAS 117
           ++S KIV++  K+N+LLPFGPLAIL+HYLT   G  F  +L+GITPLAERLGYATEQL+ 
Sbjct: 76  VQSFKIVILSNKLNLLLPFGPLAILVHYLTDNKGWFFLLSLVGITPLAERLGYATEQLSC 135

Query: 118 YTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI 177
           YTG TVGGLLNATFGN  E+IISI ALK+GMIRVVQ +LLGSILSN+LLVLGCAFF GG+
Sbjct: 136 YTGATVGGLLNATFGNVIELIISIIALKNGMIRVVQLTLLGSILSNILLVLGCAFFCGGL 195

Query: 178 VHHKNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLI 237
           V     Q F+K  AVV+SG+LLMAVMG  FP  LH+TH+E+H+G S L+LSRF SCIML+
Sbjct: 196 VFPGKDQVFDKRNAVVSSGMLLMAVMGLLFPTFLHYTHSEVHAGSSELALSRFISCIMLV 255

Query: 238 AYASFLFFQLKSQHNLYGSLDEEGD--GEVEEDPEIYAWEAIGWLAILTVWVSILSGYLV 297
           AYA++LFFQLKSQ + Y     + +     +EDPEI  WEAI WL+I T WVS+LSGYLV
Sbjct: 256 AYAAYLFFQLKSQPSFYTEKTNQNEETSNDDEDPEISKWEAIIWLSIFTAWVSLLSGYLV 315

Query: 298 DAIEGASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMF 357
           DAIEG S S  +P++FISVILLPIVGNAAEHA AIMFAMKDKL+++LGVAIGSS QISMF
Sbjct: 316 DAIEGTSVSWKIPISFISVILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSSIQISMF 375

Query: 358 VIPFCVVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQ-EGTSNYFKGLMLILCYLIV 417
            +PFCVV+GW MG  MDLN +LFETATL ITV+VVAF LQ EGTSNYFK LMLILCYLIV
Sbjct: 376 AVPFCVVIGWMMGAQMDLNLQLFETATLLITVIVVAFFLQLEGTSNYFKRLMLILCYLIV 435

Query: 418 AASFFVHVDPSSG 428
           AASFFVH DP  G
Sbjct: 436 AASFFVHEDPHQG 448

BLAST of Carg04339 vs. ExPASy TrEMBL
Match: A0A6J1GCH0 (Vacuolar cation/proton exchanger OS=Cucurbita moschata OX=3662 GN=LOC111452880 PE=3 SV=1)

HSP 1 Score: 830.9 bits (2145), Expect = 2.5e-237
Identity = 426/428 (99.53%), Postives = 427/428 (99.77%), Query Frame = 0

Query: 1   MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLES 60
           MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLES
Sbjct: 1   MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLES 60

Query: 61  AKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTG 120
           AKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTG
Sbjct: 61  AKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180

Query: 181 KNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240
           KNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA
Sbjct: 181 KNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240

Query: 241 SFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEG 300
           SFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEG
Sbjct: 241 SFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEG 300

Query: 301 ASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFC 360
           ASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFC
Sbjct: 301 ASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFC 360

Query: 361 VVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV
Sbjct: 361 VVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420

Query: 421 HVDPSSGE 429
           HVDPSS +
Sbjct: 421 HVDPSSDD 428

BLAST of Carg04339 vs. ExPASy TrEMBL
Match: A0A6J1KFA3 (Vacuolar cation/proton exchanger OS=Cucurbita maxima OX=3661 GN=LOC111493348 PE=3 SV=1)

HSP 1 Score: 829.7 bits (2142), Expect = 5.5e-237
Identity = 425/428 (99.30%), Postives = 427/428 (99.77%), Query Frame = 0

Query: 1   MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLES 60
           MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLES
Sbjct: 1   MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLES 60

Query: 61  AKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTG 120
           AKIVLIKAKIN+LLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTG
Sbjct: 61  AKIVLIKAKINLLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180

Query: 181 KNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240
           KNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA
Sbjct: 181 KNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240

Query: 241 SFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEG 300
           SFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEG
Sbjct: 241 SFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEG 300

Query: 301 ASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFC 360
           ASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFC
Sbjct: 301 ASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFC 360

Query: 361 VVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV
Sbjct: 361 VVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420

Query: 421 HVDPSSGE 429
           HVDPSS +
Sbjct: 421 HVDPSSDD 428

BLAST of Carg04339 vs. ExPASy TrEMBL
Match: A0A1S3CKH8 (Vacuolar cation/proton exchanger OS=Cucumis melo OX=3656 GN=LOC103501973 PE=3 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 3.1e-216
Identity = 393/428 (91.82%), Postives = 407/428 (95.09%), Query Frame = 0

Query: 1   MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLES 60
           MGS EE TNYLSDE+IPLK +PVAKN+ HSFDCET  VRSSP D RWWKTNNIR RPL S
Sbjct: 1   MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSP-DTRWWKTNNIRIRPLRS 60

Query: 61  AKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTG 120
           A IVL++AKINVLLPFGPLAILLHYLTGKHG VFFFTLLGITPLAERLGYATEQLA YTG
Sbjct: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI+HH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180

Query: 181 KNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240
           K VQ FNKG AVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA
Sbjct: 181 KKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240

Query: 241 SFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEG 300
           S+LFFQLKSQHNLYG LDEE DGEVE+D EI+AWEAIGWLAILTVWVSILSGYLVDAI+G
Sbjct: 241 SYLFFQLKSQHNLYGPLDEEVDGEVEDDSEIFAWEAIGWLAILTVWVSILSGYLVDAIQG 300

Query: 301 ASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFC 360
           ASESL+LPVAFISVILLPIVGNAAEHASAIMFAMKDKL+ITLGVAIGSSTQISMFVIPFC
Sbjct: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360

Query: 361 VVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMG PMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV
Sbjct: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420

Query: 421 HVDPSSGE 429
           HVDPS+ +
Sbjct: 421 HVDPSNDD 427

BLAST of Carg04339 vs. ExPASy TrEMBL
Match: C8CBQ5 (Vacuolar cation/proton exchanger OS=Cucumis sativus OX=3659 GN=CAX2 PE=2 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 5.0e-214
Identity = 389/428 (90.89%), Postives = 405/428 (94.63%), Query Frame = 0

Query: 1   MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLES 60
           MGS EE TNYLSDE+IPLK +PVAKN+ HSFDCET  VRSSP D RWWKT+NIR RPL S
Sbjct: 1   MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSP-DTRWWKTSNIRIRPLRS 60

Query: 61  AKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTG 120
           A IVL++AKINVLLPFGPLAILLHYLTGKHG VFFFTLLGITPLAERLGYATEQLA YTG
Sbjct: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI+HH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180

Query: 181 KNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240
             VQ FNKG AVVNSGLLLMAVMGTTFPAVLHFTHTELH GESALSLSRFSSCIMLIAYA
Sbjct: 181 TKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLIAYA 240

Query: 241 SFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEG 300
           S+LFFQLKSQH+LYG LDEE DGEVE+D EIYAWEAIGWLAILTVWVS+LSGYLVDAI+G
Sbjct: 241 SYLFFQLKSQHDLYGPLDEEVDGEVEDDSEIYAWEAIGWLAILTVWVSVLSGYLVDAIQG 300

Query: 301 ASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFC 360
           ASESL+LPVAFISVILLPIVGNAAEHASAIMFAMKDKL+ITLGVAIGSSTQISMFVIPFC
Sbjct: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360

Query: 361 VVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMG PMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV
Sbjct: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420

Query: 421 HVDPSSGE 429
           HVDPS+ +
Sbjct: 421 HVDPSNDD 427

BLAST of Carg04339 vs. ExPASy TrEMBL
Match: A0A6J1IRU5 (Vacuolar cation/proton exchanger OS=Cucurbita maxima OX=3661 GN=LOC111479941 PE=3 SV=1)

HSP 1 Score: 745.7 bits (1924), Expect = 1.0e-211
Identity = 387/426 (90.85%), Postives = 403/426 (94.60%), Query Frame = 0

Query: 1   MGSIEEGTNYLSDEDIPLKGTPVAKNLSHSFDCETVHVRSSPDDKRWWKTNNIRFRPLES 60
           MGS EE TNYLSDE  PLK +PVAKN+SHSFDCET  VRSSPD  RWWKTNNIR RPL S
Sbjct: 1   MGSTEEKTNYLSDEGTPLKSSPVAKNVSHSFDCETGPVRSSPDVTRWWKTNNIRIRPLRS 60

Query: 61  AKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLASYTG 120
           A IVL++AKINVLLPFGPLAILLHYLTGKHG VFFFTLLGI PLAERLGYATEQLA YTG
Sbjct: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGIAPLAERLGYATEQLAFYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180

Query: 181 KNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240
           K VQ FNKG+AVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA
Sbjct: 181 K-VQVFNKGSAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240

Query: 241 SFLFFQLKSQHNLYGSLDEEGDGEVEEDPEIYAWEAIGWLAILTVWVSILSGYLVDAIEG 300
           S+LFFQLKSQ +LYG LDE G+ EVE+D EIYAWEAIGWLAILTVWVSILSGYLVDAI+G
Sbjct: 241 SYLFFQLKSQQDLYGPLDEVGEDEVEDDSEIYAWEAIGWLAILTVWVSILSGYLVDAIQG 300

Query: 301 ASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMFVIPFC 360
           ASESL+LPVAFISVILLPIVGNAAEHASAIMFAMKDKL+ITLGVAIGSSTQISMFVIPFC
Sbjct: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360

Query: 361 VVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMG PMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKG+MLILCYLIVAASFFV
Sbjct: 361 VVVGWCMGEPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGVMLILCYLIVAASFFV 420

Query: 421 HVDPSS 427
           H+DP++
Sbjct: 421 HIDPTN 425

BLAST of Carg04339 vs. TAIR 10
Match: AT1G55730.1 (cation exchanger 5 )

HSP 1 Score: 538.5 bits (1386), Expect = 4.9e-153
Identity = 282/372 (75.81%), Postives = 320/372 (86.02%), Query Frame = 0

Query: 58  LESAKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLAS 117
           L+S KIV++  K+N+LLPFGPLAILLHYLT   G +F  +L+GITPLAERLGYATEQLA 
Sbjct: 65  LQSFKIVILSNKLNLLLPFGPLAILLHYLTDNKGWIFLLSLVGITPLAERLGYATEQLAC 124

Query: 118 YTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI 177
           YTG TVGGLLNATFGN TE+IISI+ALKSGMIRVVQ +LLGSILSNMLLVLGCAFF GG+
Sbjct: 125 YTGSTVGGLLNATFGNVTELIISIFALKSGMIRVVQLTLLGSILSNMLLVLGCAFFCGGL 184

Query: 178 VHHKNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLI 237
           V  +  Q F+KG AVVNSGLLLMAVMG  FPAVLH+TH+E+H+G S L+LSRFSSCIML+
Sbjct: 185 VFSQKEQVFDKGNAVVNSGLLLMAVMGLLFPAVLHYTHSEVHAGSSELALSRFSSCIMLV 244

Query: 238 AYASFLFFQLKSQHNLYGSLDEEGD-----GEVEEDPEIYAWEAIGWLAILTVWVSILSG 297
           AYA++LFFQLKSQ + Y  L EE +      + +EDPEI  WEAI WL+ILT WVS+LSG
Sbjct: 245 AYAAYLFFQLKSQPSSYTPLTEETNQNEETSDDDEDPEISKWEAIIWLSILTAWVSLLSG 304

Query: 298 YLVDAIEGASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQI 357
           YLVDAIEGAS S  +P++FISVILLPIVGNAAEHA AIMFAMKDKL+++LGVAIGSS QI
Sbjct: 305 YLVDAIEGASVSWKIPISFISVILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSSIQI 364

Query: 358 SMFVIPFCVVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYL 417
           SMF +PFCVV+GW MG  MDLNF+LFETATLFITV+VVAF LQEGTSNYFKGLMLILCYL
Sbjct: 365 SMFAVPFCVVIGWMMGAQMDLNFQLFETATLFITVIVVAFFLQEGTSNYFKGLMLILCYL 424

Query: 418 IVAASFFVHVDP 425
           IVAASFFVH DP
Sbjct: 425 IVAASFFVHEDP 436

BLAST of Carg04339 vs. TAIR 10
Match: AT1G55730.2 (cation exchanger 5 )

HSP 1 Score: 538.5 bits (1386), Expect = 4.9e-153
Identity = 282/372 (75.81%), Postives = 320/372 (86.02%), Query Frame = 0

Query: 58  LESAKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLAS 117
           L+S KIV++  K+N+LLPFGPLAILLHYLT   G +F  +L+GITPLAERLGYATEQLA 
Sbjct: 65  LQSFKIVILSNKLNLLLPFGPLAILLHYLTDNKGWIFLLSLVGITPLAERLGYATEQLAC 124

Query: 118 YTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI 177
           YTG TVGGLLNATFGN TE+IISI+ALKSGMIRVVQ +LLGSILSNMLLVLGCAFF GG+
Sbjct: 125 YTGSTVGGLLNATFGNVTELIISIFALKSGMIRVVQLTLLGSILSNMLLVLGCAFFCGGL 184

Query: 178 VHHKNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLI 237
           V  +  Q F+KG AVVNSGLLLMAVMG  FPAVLH+TH+E+H+G S L+LSRFSSCIML+
Sbjct: 185 VFSQKEQVFDKGNAVVNSGLLLMAVMGLLFPAVLHYTHSEVHAGSSELALSRFSSCIMLV 244

Query: 238 AYASFLFFQLKSQHNLYGSLDEEGD-----GEVEEDPEIYAWEAIGWLAILTVWVSILSG 297
           AYA++LFFQLKSQ + Y  L EE +      + +EDPEI  WEAI WL+ILT WVS+LSG
Sbjct: 245 AYAAYLFFQLKSQPSSYTPLTEETNQNEETSDDDEDPEISKWEAIIWLSILTAWVSLLSG 304

Query: 298 YLVDAIEGASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQI 357
           YLVDAIEGAS S  +P++FISVILLPIVGNAAEHA AIMFAMKDKL+++LGVAIGSS QI
Sbjct: 305 YLVDAIEGASVSWKIPISFISVILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSSIQI 364

Query: 358 SMFVIPFCVVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYL 417
           SMF +PFCVV+GW MG  MDLNF+LFETATLFITV+VVAF LQEGTSNYFKGLMLILCYL
Sbjct: 365 SMFAVPFCVVIGWMMGAQMDLNFQLFETATLFITVIVVAFFLQEGTSNYFKGLMLILCYL 424

Query: 418 IVAASFFVHVDP 425
           IVAASFFVH DP
Sbjct: 425 IVAASFFVHEDP 436

BLAST of Carg04339 vs. TAIR 10
Match: AT3G13320.1 (cation exchanger 2 )

HSP 1 Score: 537.3 bits (1383), Expect = 1.1e-152
Identity = 282/372 (75.81%), Postives = 317/372 (85.22%), Query Frame = 0

Query: 58  LESAKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLAS 117
           L S KIV+   K+N+LLPFGPLAIL+HY+    G VF  TL+GITPLAERLGYATEQLA 
Sbjct: 65  LNSIKIVIFCNKLNLLLPFGPLAILVHYMIDSKGWVFLLTLVGITPLAERLGYATEQLAC 124

Query: 118 YTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI 177
           YTGPTVGGLLNATFGN TE+IISI+ALK+GMIRVVQ +LLGSILSNMLLVLGCAFF GG+
Sbjct: 125 YTGPTVGGLLNATFGNVTELIISIFALKNGMIRVVQLTLLGSILSNMLLVLGCAFFCGGL 184

Query: 178 VHHKNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLI 237
           V ++  Q F+KG A VNSGLLLMAVMG  FPAVLH+TH+E+H+G S L+LSRFSSCIMLI
Sbjct: 185 VFYQKDQVFDKGIATVNSGLLLMAVMGILFPAVLHYTHSEVHAGSSELALSRFSSCIMLI 244

Query: 238 AYASFLFFQLKSQHNLYGSLDEEGDGEVE-----EDPEIYAWEAIGWLAILTVWVSILSG 297
           AYA++LFFQLKSQ N Y  LDEE +   E     EDPEI  WEAI WL+ILT WVS+LSG
Sbjct: 245 AYAAYLFFQLKSQSNSYSPLDEESNQNEETSAEDEDPEISKWEAIIWLSILTAWVSLLSG 304

Query: 298 YLVDAIEGASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQI 357
           YLVDAIEGAS S ++P+AFIS ILLPIVGNAAEHA AIMFAMKDKL+++LGVAIGSS QI
Sbjct: 305 YLVDAIEGASVSWNIPIAFISTILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSSIQI 364

Query: 358 SMFVIPFCVVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYL 417
           SMF +PFCVV+GW MG  MDLNF+LFETA LFITV+VVAF LQEG+SNYFKGLMLILCYL
Sbjct: 365 SMFAVPFCVVIGWMMGQQMDLNFQLFETAMLFITVIVVAFFLQEGSSNYFKGLMLILCYL 424

Query: 418 IVAASFFVHVDP 425
           IVAASFFVH DP
Sbjct: 425 IVAASFFVHEDP 436

BLAST of Carg04339 vs. TAIR 10
Match: AT1G55720.1 (cation exchanger 6 )

HSP 1 Score: 498.0 bits (1281), Expect = 7.3e-141
Identity = 263/373 (70.51%), Postives = 305/373 (81.77%), Query Frame = 0

Query: 58  LESAKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLAS 117
           ++S KIV++  K+N+LLPFGPLAIL+HYLT   G  F  +L+GITPLAERLGYATEQL+ 
Sbjct: 76  VQSFKIVILSNKLNLLLPFGPLAILVHYLTDNKGWFFLLSLVGITPLAERLGYATEQLSC 135

Query: 118 YTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI 177
           YTG TVGGLLNATFGN  E+IISI ALK+GMIRVVQ +LLGSILSN+LLVLGCAFF GG+
Sbjct: 136 YTGATVGGLLNATFGNVIELIISIIALKNGMIRVVQLTLLGSILSNILLVLGCAFFCGGL 195

Query: 178 VHHKNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLI 237
           V     Q F+K  AVV+SG+LLMAVMG  FP  LH+TH+E+H+G S L+LSRF SCIML+
Sbjct: 196 VFPGKDQVFDKRNAVVSSGMLLMAVMGLLFPTFLHYTHSEVHAGSSELALSRFISCIMLV 255

Query: 238 AYASFLFFQLKSQHNLYGSLDEEGD--GEVEEDPEIYAWEAIGWLAILTVWVSILSGYLV 297
           AYA++LFFQLKSQ + Y     + +     +EDPEI  WEAI WL+I T WVS+LSGYLV
Sbjct: 256 AYAAYLFFQLKSQPSFYTEKTNQNEETSNDDEDPEISKWEAIIWLSIFTAWVSLLSGYLV 315

Query: 298 DAIEGASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGSSTQISMF 357
           DAIEG S S  +P++FISVILLPIVGNAAEHA AIMFAMKDKL+++LGVAIGSS QISMF
Sbjct: 316 DAIEGTSVSWKIPISFISVILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSSIQISMF 375

Query: 358 VIPFCVVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQ-EGTSNYFKGLMLILCYLIV 417
            +PFCVV+GW MG  MDLN +LFETATL ITV+VVAF LQ EGTSNYFK LMLILCYLIV
Sbjct: 376 AVPFCVVIGWMMGAQMDLNLQLFETATLLITVIVVAFFLQLEGTSNYFKRLMLILCYLIV 435

Query: 418 AASFFVHVDPSSG 428
           AASFFVH DP  G
Sbjct: 436 AASFFVHEDPHQG 448

BLAST of Carg04339 vs. TAIR 10
Match: AT3G51860.1 (cation exchanger 3 )

HSP 1 Score: 337.4 bits (864), Expect = 1.7e-92
Identity = 190/380 (50.00%), Postives = 262/380 (68.95%), Query Frame = 0

Query: 58  LESAKIVLIKAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLAS 117
           L + + V++  K+ +L    PLAIL +       L+F  +L+G+TPLAER+ + TEQLA 
Sbjct: 63  LSNLQEVILGTKLTLLFLAIPLAILANSYNYGRPLIFGLSLIGLTPLAERVSFLTEQLAF 122

Query: 118 YTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI 177
           YTGPTVGGLLNAT GNATE+II+I AL +  + VV+ SLLGSILSN+LLVLG + F GGI
Sbjct: 123 YTGPTVGGLLNATCGNATELIIAILALANNKVAVVKYSLLGSILSNLLLVLGTSLFFGGI 182

Query: 178 VHHKNVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHS----GESALSLSRFSSC 237
            + +  Q F++  A VN  LLLM ++    P +L +  T   S     + +L+LSR SS 
Sbjct: 183 ANIRREQRFDRKQADVNFFLLLMGLLCHLLPLLLKYAATGEVSTSMINKMSLTLSRTSSI 242

Query: 238 IMLIAYASFLFFQLKSQHNLYGSLDEEGDG-----EVEEDPEIYAWEAIGWLAILTVWVS 297
           +MLIAY ++L FQL +   L+ +  ++ D       VEE P I  W    WL  +T+ ++
Sbjct: 243 VMLIAYIAYLIFQLWTHRQLFEAQQDDDDAYDDEVSVEETPVIGFWSGFAWLVGMTIVIA 302

Query: 298 ILSGYLVDAIEGASESLHLPVAFISVILLPIVGNAAEHASAIMFAMKDKLNITLGVAIGS 357
           +LS Y+VD IE AS+S  L V+FIS+ILLPIVGNAAEHA AI+FA K+KL+I+LGVA+GS
Sbjct: 303 LLSEYVVDTIEDASDSWGLSVSFISIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGS 362

Query: 358 STQISMFVIPFCVVVGWCMGIPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLI 417
           +TQIS+FV+P  V+V W +GI MDLNF + ET++L + +++ AF LQ+GTS+Y KGL+L+
Sbjct: 363 ATQISLFVVPLSVIVAWILGIKMDLNFNILETSSLALAIIITAFTLQDGTSHYMKGLVLL 422

Query: 418 LCYLIVAASFFVHVDPSSGE 429
           LCY+I+AA FFV   P   +
Sbjct: 423 LCYVIIAACFFVDQIPQPND 442

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7036402.13.5e-238100.00Vacuolar cation/proton exchanger 2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022949566.15.1e-23799.53vacuolar cation/proton exchanger 3-like [Cucurbita moschata] >XP_022949567.1 vac... [more]
KAG6606682.15.1e-23799.53Vacuolar cation/proton exchanger 2, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022998783.11.1e-23699.30vacuolar cation/proton exchanger 3-like [Cucurbita maxima] >XP_022998784.1 vacuo... [more]
XP_008463979.16.5e-21691.82PREDICTED: vacuolar cation/proton exchanger 3-like [Cucumis melo] >XP_008463980.... [more]
Match NameE-valueIdentityDescription
Q5KQN02.8e-16172.60Vacuolar cation/proton exchanger 2 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
Q6K1C43.2e-15775.13Vacuolar cation/proton exchanger 3 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
Q8L7836.9e-15275.81Vacuolar cation/proton exchanger 5 OS=Arabidopsis thaliana OX=3702 GN=CAX5 PE=2 ... [more]
Q392541.5e-15175.81Vacuolar cation/proton exchanger 2 OS=Arabidopsis thaliana OX=3702 GN=CAX2 PE=1 ... [more]
Q9LFZ81.0e-13970.51Putative vacuolar cation/proton exchanger 6 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Match NameE-valueIdentityDescription
A0A6J1GCH02.5e-23799.53Vacuolar cation/proton exchanger OS=Cucurbita moschata OX=3662 GN=LOC111452880 P... [more]
A0A6J1KFA35.5e-23799.30Vacuolar cation/proton exchanger OS=Cucurbita maxima OX=3661 GN=LOC111493348 PE=... [more]
A0A1S3CKH83.1e-21691.82Vacuolar cation/proton exchanger OS=Cucumis melo OX=3656 GN=LOC103501973 PE=3 SV... [more]
C8CBQ55.0e-21490.89Vacuolar cation/proton exchanger OS=Cucumis sativus OX=3659 GN=CAX2 PE=2 SV=1[more]
A0A6J1IRU51.0e-21190.85Vacuolar cation/proton exchanger OS=Cucurbita maxima OX=3661 GN=LOC111479941 PE=... [more]
Match NameE-valueIdentityDescription
AT1G55730.14.9e-15375.81cation exchanger 5 [more]
AT1G55730.24.9e-15375.81cation exchanger 5 [more]
AT3G13320.11.1e-15275.81cation exchanger 2 [more]
AT1G55720.17.3e-14170.51cation exchanger 6 [more]
AT3G51860.11.7e-9250.00cation exchanger 3 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044880NCX, central ion-binding domain superfamilyGENE3D1.20.1420.30coord: 121..260
e-value: 4.9E-15
score: 57.5
coord: 265..420
e-value: 2.1E-17
score: 65.3
IPR004837Sodium/calcium exchanger membrane regionPFAMPF01699Na_Ca_excoord: 277..416
e-value: 2.5E-18
score: 66.5
coord: 92..247
e-value: 1.9E-20
score: 73.3
NoneNo IPR availableTIGRFAMTIGR00846TIGR00846coord: 60..419
e-value: 1.6E-114
score: 380.5
NoneNo IPR availablePANTHERPTHR31503:SF54VACUOLAR CATION/PROTON EXCHANGERcoord: 5..426
IPR004798Calcium/proton exchanger CAX-likeTIGRFAMTIGR00378TIGR00378coord: 73..419
e-value: 5.7E-103
score: 342.7
IPR004713Calcium/proton exchangerPANTHERPTHR31503VACUOLAR CALCIUM ION TRANSPORTERcoord: 5..426

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg04339-RACarg04339-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070588 calcium ion transmembrane transport
biological_process GO:0006874 cellular calcium ion homeostasis
biological_process GO:0006816 calcium ion transport
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane
molecular_function GO:0015369 calcium:proton antiporter activity
molecular_function GO:0008324 cation transmembrane transporter activity