Carg04310 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04310
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	mediator of RNA polymerase II transcription subunit 9-like
Location	Carg_Chr01: 273823 .. 277133 (+)
RNA-Seq Expression	Carg04310
Synteny	Carg04310

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTTCTCTGGAGGAGGAAACTGGAGCATGATCCCCAACATCCCTACTCTCAGCAACTCCTCTAATCTTTCCAATCAAGAATCCCTTTACCTTCAACAGCAGCAATTCTCCCTTCAGCAGCAGCAGCAGCAACAACAGCAACTATCCTTTCACCACCACCCACAGCAATTCCAGCCCCAAATTCAACCCCAACAGCAATTTCAGCAGCAACAATCCTCCCCCCAATTTCAACAGCTACAACCCTCTCCTCAAATTCAGCCCCAACAACAACTCTCCACCCAATTCCAGCAGCAAATGCAACCCCAACATTTCCCCCAGCAGCAGCAGCAGCAACAACAACAACAGCAATATCATTTTCAACAACAACAGCAGCGCCTTCTTCAACAGCAGCAGCAGCAGCAGCAGCAACAGAAACAGCAGCAATATCATCAACACCAGTCTCTGGCTTCCCATTTTCATCTATTTCATGTAATGTCTTCAGAAACCCCTTAATTTTGAGTGTTCTCTAATCCCCGTGTTTTGCGTTTGTTGACTTTCTTGGATGTTTTGTGTGTTGTAGTTTGTGGAGAAATTAGCGGATGCGATTGAGACTGGAGTTAGAGATCAGCAATCGGATGCATTGGTATCAATTTTGATTCTTTTTTGAAATTTTCTCTCAAGATTAACCATACCGTTGTTTCCTCTAATAATTTGTTCATTTAACGTTTTGGAAGGTCAATGATCTGAACAACCACTTTGAGAAGTGCCAGCAACTGTTAAACTCGATATCTGGATCACTCAGCTCAAAGGCTATGGTAAAGTTGAAATGACAAATCCATCACTACAAATGAGATATCACAATTTTTTTTCCTTTTTTTCTTTTGGTTGCAGACAGTTGAGGGACAGAAGAAGAAGCTGGAAGAACATGAGCAGTTGTTAAGTTATCGAAGGTATGGTGGTAACCAATTTTTGGCTGTGGTTTGCATTAAACTGTCTTCTTCATCAATGGTCCTACTGATGATTCTCTATTAAAAAAGGGAACTGATTGGCAAATATAAGAGCTCCGTTGAAGAGCTTGTCAAGGGCGAGCCTTAGCTACTTCTCACCCATTCCTAATTAGGTAATACTTCAATTTATATTTACAGTTTCTTTTCCGCTTATCTCAATTGTCCAAGGTTCTGTTTGGCTTTCCTTTTCAATGTTGTCATCTTTTGCTAAATGGTTTGGTTGTGGGGTTGAAATTGAAAAGTGGTTATCAGGAAAGAAATTCACACACCAAAACATAAATTATTGTACGAATATGAGCTTTTAAGATTAGTTTTTGTACATTCTCAACACTAAATGTTGTTTAGGTATCACCTTAGGACTGAGTTGAGGGTCATAGCGTTTCTGGTCTAAAAGTGGGTTCGAAGTTGAATACAATTCAACATTCGAGATTGTGTGTTGTATTGTTCTAGATGTCTTAGGTGTTAGCTGCATAAATAGTCAATCCACACTTTTTGTCGACATGTTTTGGGATGCGAGAAAAAGGAATGTAGAGTTACATTTCGGAGAGAGTAAACCAAACGCTTGTATATGGATATGAATTTTCAATCGATTCAACGCCATCTGTGCATTCCTTCAATTTTGGTTCAAATTCATTTAAATTGTCTCTTATCAAAAGAAAGGAAAAGGAAACTTGAAGCACATACTTATGAATTCTACACCATTTCAGGCCGGGCTAGCATCATGTACCTGTTTTGAAGGGACGTGAAAAGAGCTGCTTTCACCGGGTCTTGCAATTCCATGGAGGGGCTTTTATCTAATCTGCCTCCCTGCATTTCATAACACGATGATATGATGCACAGTTGAATTCAATCCTCTCTGTTCTCTTCGACTTTTTCAGGAACTTATGTTACCTCTAACGTATCTTGTACAGTTATTCCAACAGTTAACTTCTAAAATTAATGAACAGTAGATGCTTTCTCTAGTTTTCATTCTTTTAAATGTTAAATTTGGATTGATGCGAATATTAAGTTATAAAACATCATGAAGCTTAACCCAAATTTCAGGTATCATATGCAATTTCCTACGTCGATAAAGAATGTACCATGGAAATTCATAAAGATATCACCATCTCTCCTAGATGAACCTGTAACATCTTGTCCTTTCCCCATTCTACTTCTTTGATCTTCCTACCAGCACAATGATAGGATAAACCATCATCAGCTTCACACATATGGAGAGAGCTTTCTAGTAAAAGATTTCGGTAATGTTGTTCTTGAACATTCTATTCAATAATACTATCATCAAAATCTCGGCTTTCAATCTGAAGAGCCAAATCATGAAGAATTTCATCAACTCTCTCTAAACCTTCTTGCATTGAGTTTCCTGATTTGAACATATAAACCCTTTTCTTCACCTCAATCCAGCTGCACCCAGGAACTTTCTTTAAACCCTTCATCCTTGCCGAGATCCTGACCCTCGCAGAATCCTCCCACCTACAGCTTGCAGAAAAAATATTCGAGAGCAACATATGGCTTCCCGTTATCTCGGAATTCAGATTTAAAATCTGAGAGCCAGTTTCTTCTGCAAGATCTGTATCCTTGTGCATCCTGCAAGAGTTGAGAAGAGCACTCCAGATATAAGCATTGGGTTTGATTGGCATGCTCTTGACTATGTTACTTGCTTCTTCCACGAGCCCAGCACGACCTAGAAGATCGACCATGCACGCATAGTGCTCCATCTGAGGTTTGATCTTGAAGTTCTGTAGCATCTGATCGAAAAGCCAACGGCCTTCGGCAACAAGACCGGCATGACTACAAGCAGAAAGAGCAGCAATAAAGGTAACATCATCTGGTCTAAATCCTGATTTGATCATCTCGTCAAAAGTTACGAGAGCATCTTTACCAAGTCCATGCATTCCATATCCTGCAATCATTGAGTTCCATGAGATTAAATCTCGATTTTCTAGTTTTCCGAACACCAAACAGCCTGGCTTGAAACTTCCACATTTTGTATACATGTTAATCAATCCATTTCCTACCAGTATGTTATCTTCCATCCGAGCTCTAATCACATGACCGTGCATTTCCCTACCAAGATTCAGAGCTGCTAGCATAGCACAAATTGATAGAACACTAGATATCGTCACTGAGTTCGCTTTTACATTTGCAAGCTGCATTTGGCGGAAAACTTCCAAAGATTCTTCTCCAAATCCATTAGAAGCAAATCCACAAATGACTGCGCTCCAAGTTATGACATTAGGTTTCATCTCTGGAGAGCCTTCCATTTTCTCAAGCTCAGAAAACGCTTCAAAAGCTTTGTCATATAATCCAGATTCAGCATAA

mRNA sequence

ATGGATTTCTCTGGAGGAGGAAACTGGAGCATGATCCCCAACATCCCTACTCTCAGCAACTCCTCTAATCTTTCCAATCAAGAATCCCTTTACCTTCAACAGCAGCAATTCTCCCTTCAGCAGCAGCAGCAGCAACAACAGCAACTATCCTTTCACCACCACCCACAGCAATTCCAGCCCCAAATTCAACCCCAACAGCAATTTCAGCAGCAACAATCCTCCCCCCAATTTCAACAGCTACAACCCTCTCCTCAAATTCAGCCCCAACAACAACTCTCCACCCAATTCCAGCAGCAAATGCAACCCCAACATTTCCCCCAGCAGCAGCAGCAGCAACAACAACAACAGCAATATCATTTTCAACAACAACAGCAGCGCCTTCTTCAACAGCAGCAGCAGCAGCAGCAGCAACAGAAACAGCAGCAATATCATCAACACCAGTCTCTGGCTTCCCATTTTCATCTATTTCATTTTGTGGAGAAATTAGCGGATGCGATTGAGACTGGAGTTAGAGATCAGCAATCGGATGCATTGGTCAATGATCTGAACAACCACTTTGAGAAGTGCCAGCAACTGTTAAACTCGATATCTGGATCACTCAGCTCAAAGGCTATGACAGTTGAGGGACAGAAGAAGAAGCTGGAAGAACATGAGCAGTTGTTAAGTTATCGAAGAGCTGCTAGCATAGCACAAATTGATAGAACACTAGATATCGTCACTGAGTTCGCTTTTACATTTGCAAGCTGCATTTGGCGGAAAACTTCCAAAGATTCTTCTCCAAATCCATTAGAAGCAAATCCACAAATGACTGCGCTCCAAGTTATGACATTAGGTTTCATCTCTGGAGAGCCTTCCATTTTCTCAAGCTCAGAAAACGCTTCAAAAGCTTTGTCATATAATCCAGATTCAGCATAA

Coding sequence (CDS)

ATGGATTTCTCTGGAGGAGGAAACTGGAGCATGATCCCCAACATCCCTACTCTCAGCAACTCCTCTAATCTTTCCAATCAAGAATCCCTTTACCTTCAACAGCAGCAATTCTCCCTTCAGCAGCAGCAGCAGCAACAACAGCAACTATCCTTTCACCACCACCCACAGCAATTCCAGCCCCAAATTCAACCCCAACAGCAATTTCAGCAGCAACAATCCTCCCCCCAATTTCAACAGCTACAACCCTCTCCTCAAATTCAGCCCCAACAACAACTCTCCACCCAATTCCAGCAGCAAATGCAACCCCAACATTTCCCCCAGCAGCAGCAGCAGCAACAACAACAACAGCAATATCATTTTCAACAACAACAGCAGCGCCTTCTTCAACAGCAGCAGCAGCAGCAGCAGCAACAGAAACAGCAGCAATATCATCAACACCAGTCTCTGGCTTCCCATTTTCATCTATTTCATTTTGTGGAGAAATTAGCGGATGCGATTGAGACTGGAGTTAGAGATCAGCAATCGGATGCATTGGTCAATGATCTGAACAACCACTTTGAGAAGTGCCAGCAACTGTTAAACTCGATATCTGGATCACTCAGCTCAAAGGCTATGACAGTTGAGGGACAGAAGAAGAAGCTGGAAGAACATGAGCAGTTGTTAAGTTATCGAAGAGCTGCTAGCATAGCACAAATTGATAGAACACTAGATATCGTCACTGAGTTCGCTTTTACATTTGCAAGCTGCATTTGGCGGAAAACTTCCAAAGATTCTTCTCCAAATCCATTAGAAGCAAATCCACAAATGACTGCGCTCCAAGTTATGACATTAGGTTTCATCTCTGGAGAGCCTTCCATTTTCTCAAGCTCAGAAAACGCTTCAAAAGCTTTGTCATATAATCCAGATTCAGCATAA

Protein sequence

MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSLQQQQQQQQQLSFHHHPQQFQPQIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFPQQQQQQQQQQQYHFQQQQQRLLQQQQQQQQQQKQQQYHQHQSLASHFHLFHFVEKLADAIETGVRDQQSDALVNDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRRAASIAQIDRTLDIVTEFAFTFASCIWRKTSKDSSPNPLEANPQMTALQVMTLGFISGEPSIFSSSENASKALSYNPDSA

Homology

[Close]

BLAST of Carg04310 vs. NCBI nr
Match: KAG7036431.1 (Mediator of RNA polymerase II transcription subunit 9, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 522.7 bits (1345), Expect = 2.1e-144
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0


Query: 1   MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSLQQQQQQQQQLSFHHHPQQFQP 60
           MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSLQQQQQQQQQLSFHHHPQQFQP
Sbjct: 1   MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSLQQQQQQQQQLSFHHHPQQFQP 60

Query: 61  QIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFPQQQQQQQQQQQYHF 120
           QIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFPQQQQQQQQQQQYHF
Sbjct: 61  QIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFPQQQQQQQQQQQYHF 120

Query: 121 QQQQQRLLQQQQQQQQQQKQQQYHQHQSLASHFHLFHFVEKLADAIETGVRDQQSDALVN 180
           QQQQQRLLQQQQQQQQQQKQQQYHQHQSLASHFHLFHFVEKLADAIETGVRDQQSDALVN
Sbjct: 121 QQQQQRLLQQQQQQQQQQKQQQYHQHQSLASHFHLFHFVEKLADAIETGVRDQQSDALVN 180

Query: 181 DLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRRAASIAQIDRTLDIVT 240
           DLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRRAASIAQIDRTLDIVT
Sbjct: 181 DLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRRAASIAQIDRTLDIVT 240

Query: 241 EFAFTFASCIWRKTSKDSSPNPLEANPQMTALQVMTLGFISGEPSIFSSSENASKALSYN 300
           EFAFTFASCIWRKTSKDSSPNPLEANPQMTALQVMTLGFISGEPSIFSSSENASKALSYN
Sbjct: 241 EFAFTFASCIWRKTSKDSSPNPLEANPQMTALQVMTLGFISGEPSIFSSSENASKALSYN 300

Query: 301 PDSA 305
           PDSA
Sbjct: 301 PDSA 304

[Close]

BLAST of Carg04310 vs. NCBI nr
Match: KAG6606716.1 (hypothetical protein SDJN03_00058, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 358.2 bits (918), Expect = 6.9e-95
Identity = 222/226 (98.23%), Postives = 222/226 (98.23%), Query Frame = 0


Query: 1   MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSL-QQQQQQQQQLSFHHHPQQFQ 60
           MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSL QQQQQQQQQLSFHH PQQFQ
Sbjct: 1   MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSLQQQQQQQQQQLSFHHQPQQFQ 60

Query: 61  PQIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFPQQQQQQQQQQQYH 120
           PQIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFPQQQQQQQQQQQYH
Sbjct: 61  PQIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFPQQQQQQQQQQQYH 120

Query: 121 FQQQQQRLLQQQQQQQQQQKQQQYHQHQSLASHFHLFHFVEKLADAIETGVRDQQSDALV 180
           FQQQQQRLL  QQQQQQQQKQQQYHQHQSLASHFHLFHFVEKLADAIETGVRDQQSDALV
Sbjct: 121 FQQQQQRLL--QQQQQQQQKQQQYHQHQSLASHFHLFHFVEKLADAIETGVRDQQSDALV 180

Query: 181 NDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRR 226
           NDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRR
Sbjct: 181 NDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRR 224

[Close]

BLAST of Carg04310 vs. NCBI nr
Match: XP_023523773.1 (mediator of RNA polymerase II transcription subunit 9-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 355.1 bits (910), Expect = 5.8e-94
Identity = 220/226 (97.35%), Postives = 220/226 (97.35%), Query Frame = 0


Query: 1   MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSL-QQQQQQQQQLSFHHHPQQFQ 60
           MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSL QQQQQQQQQLSFHH PQQFQ
Sbjct: 1   MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSLQQQQQQQQQQLSFHHQPQQFQ 60

Query: 61  PQIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFPQQQQQQQQQQQYH 120
           PQIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFPQQQQQQQQQQQYH
Sbjct: 61  PQIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFPQQQQQQQQQQQYH 120

Query: 121 FQQQQQRLLQQQQQQQQQQKQQQYHQHQSLASHFHLFHFVEKLADAIETGVRDQQSDALV 180
           FQQQQQRLL   QQQQQQQKQQQYHQHQSLASHFHLFH VEKLADAIETGVRDQQSDALV
Sbjct: 121 FQQQQQRLL---QQQQQQQKQQQYHQHQSLASHFHLFHLVEKLADAIETGVRDQQSDALV 180

Query: 181 NDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRR 226
           NDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRR
Sbjct: 181 NDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRR 223

[Close]

BLAST of Carg04310 vs. NCBI nr
Match: XP_022949500.1 (mediator of RNA polymerase II transcription subunit 9-like [Cucurbita moschata])

HSP 1 Score: 350.5 bits (898), Expect = 1.4e-92
Identity = 220/227 (96.92%), Postives = 220/227 (96.92%), Query Frame = 0


Query: 1   MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSL-QQQQQQQQQLSFHHHPQQFQ 60
           MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSL QQQQQQQQQLSFHH PQQFQ
Sbjct: 1   MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSLQQQQQQQQQQLSFHHQPQQFQ 60

Query: 61  PQIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFP-QQQQQQQQQQQY 120
           PQIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFP QQQQQQQQQQQY
Sbjct: 61  PQIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFPQQQQQQQQQQQQY 120

Query: 121 HFQQQQQRLLQQQQQQQQQQKQQQYHQHQSLASHFHLFHFVEKLADAIETGVRDQQSDAL 180
           HFQQQQQRLL   QQQQQQQKQQQYHQHQSLASHFHLFH VEKLADAIETGVRDQQSDAL
Sbjct: 121 HFQQQQQRLL---QQQQQQQKQQQYHQHQSLASHFHLFHLVEKLADAIETGVRDQQSDAL 180

Query: 181 VNDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRR 226
           VNDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRR
Sbjct: 181 VNDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRR 224

[Close]

BLAST of Carg04310 vs. NCBI nr
Match: XP_022997827.1 (mediator of RNA polymerase II transcription subunit 9-like [Cucurbita maxima])

HSP 1 Score: 349.0 bits (894), Expect = 4.2e-92
Identity = 219/228 (96.05%), Postives = 219/228 (96.05%), Query Frame = 0


Query: 1   MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSL---QQQQQQQQQLSFHHHPQQ 60
           MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSL   QQQQQQQQQLSFHH PQQ
Sbjct: 1   MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSLQQQQQQQQQQQQLSFHHQPQQ 60

Query: 61  FQPQIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFPQQQQQQQQQQQ 120
           FQPQIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFP QQQQQQQQQQ
Sbjct: 61  FQPQIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFP-QQQQQQQQQQ 120

Query: 121 YHFQQQQQRLLQQQQQQQQQQKQQQYHQHQSLASHFHLFHFVEKLADAIETGVRDQQSDA 180
           YHFQQQQQRLL   QQQQQQQKQQQYHQHQSLASHFHLFH VEKLADAIETGVRDQQSDA
Sbjct: 121 YHFQQQQQRLL---QQQQQQQKQQQYHQHQSLASHFHLFHLVEKLADAIETGVRDQQSDA 180

Query: 181 LVNDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRR 226
           LVNDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRR
Sbjct: 181 LVNDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRR 224

[Close]

BLAST of Carg04310 vs. ExPASy Swiss-Prot
Match: Q8RWA2 (Mediator of RNA polymerase II transcription subunit 9 OS=Arabidopsis thaliana OX=3702 GN=MED9 PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 6.2e-30
Identity = 127/237 (53.59%), Postives = 142/237 (59.92%), Query Frame = 0


Query: 5   GGGNWSMIPNIPTLSNSSNLSN-QESLYLQQQQFSLQQQQQQQQQLSFHHHPQQFQPQIQ 64
           GGGNWSMIPN+    N    +N  + L+LQQQQ  LQQQQQQQQQ  FH   QQ   Q Q
Sbjct: 7   GGGNWSMIPNVQAQGNFGTPTNHDQQLFLQQQQ--LQQQQQQQQQQQFHLQQQQQTQQQQ 66

Query: 65  PQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFPQQQQQQQQQQQYHFQQQ 124
            Q Q QQQQ   Q+QQ Q       QQQ   Q +    P   PQ  Q    QQ      Q
Sbjct: 67  QQFQPQQQQEMQQYQQFQQQQHFIQQQQFQQQQRLLQSPPLQPQSLQSPPPQQTMVHTPQ 126

Query: 125 QQRLLQQQQQQQQQQKQQQYHQHQSLASHFHLFHFVEKLADAIETGVRDQQSDALVNDLN 184
                 QQQQQ  Q   Q   QHQSLASHFHL+  VEKLAD IE G RDQ SDALVN+LN
Sbjct: 127 SMMHTPQQQQQLVQTPVQTPQQHQSLASHFHLYPMVEKLADVIENGTRDQNSDALVNELN 186

Query: 185 NHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRRAASIAQIDRTLDIVT 241
           +HF+KCQQLLNSISGSL SK MTV+GQK+ +EE EQLL  RR   +       +IVT
Sbjct: 187 SHFDKCQQLLNSISGSLGSKTMTVDGQKRNVEESEQLLQQRRDLIVEYRKSIEEIVT 241

[Close]

BLAST of Carg04310 vs. ExPASy Swiss-Prot
Match: Q55F68 (Adenylate cyclase, terminal-differentiation specific OS=Dictyostelium discoideum OX=44689 GN=acrA PE=1 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 5.8e-04
Identity = 88/206 (42.72%), Postives = 104/206 (50.49%), Query Frame = 0


Query: 27   QESLYLQQQQFSLQQQQQQQQQLSFHHHPQQFQPQIQPQQQFQQQ-QSSPQFQQLQPSPQ 86
            Q+    QQQQ   QQQQQQQQQ      PQQ Q Q+Q QQQ QQQ Q SPQ QQ    PQ
Sbjct: 1926 QQQQQQQQQQQQQQQQQQQQQQQQQQQQPQQ-QQQLQQQQQHQQQKQPSPQQQQQPQQPQ 1985

Query: 87   IQPQQQLSTQFQQQMQPQHFPQQQQQQQQQQQYHFQQQQQRLLQQQQQQQQQQKQQQYHQ 146
             Q QQQ+  Q+Q Q+Q Q   QQQQQQQQQQQ   QQQQQ+  QQQQQQQQQQ+QQQ  Q
Sbjct: 1986 QQQQQQIQNQYQHQLQYQRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQ 2045

Query: 147  HQSLASHFHLFHFVEKLADAIETGVRDQQSDALVNDLNNHFEKCQQLLNSISGSLSSKAM 206
                  H H  H  ++     +   +  Q               QQ          S+  
Sbjct: 2046 -----QHHHHHHQQQQFQQQSQQSQQQSQQ--------------QQQQQQQQSQQQSQQQ 2105

Query: 207  TVEGQKKKLEEHEQLLSYRRAASIAQ 232
            + + QKK    H Q +  +R  S  Q
Sbjct: 2106 SQQIQKKSQHPHSQQIQSQRHQSQPQ 2111

[Close]

BLAST of Carg04310 vs. ExPASy TrEMBL
Match: A0A6J1GCY0 (mediator of RNA polymerase II transcription subunit 9-like OS=Cucurbita moschata OX=3662 GN=LOC111452828 PE=4 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 7.0e-93
Identity = 220/227 (96.92%), Postives = 220/227 (96.92%), Query Frame = 0


Query: 1   MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSL-QQQQQQQQQLSFHHHPQQFQ 60
           MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSL QQQQQQQQQLSFHH PQQFQ
Sbjct: 1   MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSLQQQQQQQQQQLSFHHQPQQFQ 60

Query: 61  PQIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFP-QQQQQQQQQQQY 120
           PQIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFP QQQQQQQQQQQY
Sbjct: 61  PQIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFPQQQQQQQQQQQQY 120

Query: 121 HFQQQQQRLLQQQQQQQQQQKQQQYHQHQSLASHFHLFHFVEKLADAIETGVRDQQSDAL 180
           HFQQQQQRLL   QQQQQQQKQQQYHQHQSLASHFHLFH VEKLADAIETGVRDQQSDAL
Sbjct: 121 HFQQQQQRLL---QQQQQQQKQQQYHQHQSLASHFHLFHLVEKLADAIETGVRDQQSDAL 180

Query: 181 VNDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRR 226
           VNDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRR
Sbjct: 181 VNDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRR 224

[Close]

BLAST of Carg04310 vs. ExPASy TrEMBL
Match: A0A6J1K651 (mediator of RNA polymerase II transcription subunit 9-like OS=Cucurbita maxima OX=3661 GN=LOC111492669 PE=4 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 2.0e-92
Identity = 219/228 (96.05%), Postives = 219/228 (96.05%), Query Frame = 0


Query: 1   MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSL---QQQQQQQQQLSFHHHPQQ 60
           MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSL   QQQQQQQQQLSFHH PQQ
Sbjct: 1   MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSLQQQQQQQQQQQQLSFHHQPQQ 60

Query: 61  FQPQIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFPQQQQQQQQQQQ 120
           FQPQIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFP QQQQQQQQQQ
Sbjct: 61  FQPQIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFP-QQQQQQQQQQ 120

Query: 121 YHFQQQQQRLLQQQQQQQQQQKQQQYHQHQSLASHFHLFHFVEKLADAIETGVRDQQSDA 180
           YHFQQQQQRLL   QQQQQQQKQQQYHQHQSLASHFHLFH VEKLADAIETGVRDQQSDA
Sbjct: 121 YHFQQQQQRLL---QQQQQQQKQQQYHQHQSLASHFHLFHLVEKLADAIETGVRDQQSDA 180

Query: 181 LVNDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRR 226
           LVNDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRR
Sbjct: 181 LVNDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRR 224

[Close]

BLAST of Carg04310 vs. ExPASy TrEMBL
Match: A0A6J1FZC2 (mediator of RNA polymerase II transcription subunit 9-like OS=Cucurbita moschata OX=3662 GN=LOC111449284 PE=4 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 1.2e-79
Identity = 203/229 (88.65%), Postives = 207/229 (90.39%), Query Frame = 0


Query: 1   MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSL---QQQQQQQQQLSFHHHPQQ 60
           MDFS GGNWSMIPNIP LSNSSNLSNQESLYLQQQQFSL   QQQQQQQQQ SFHH  QQ
Sbjct: 1   MDFS-GGNWSMIPNIPNLSNSSNLSNQESLYLQQQQFSLQQQQQQQQQQQQQSFHHQQQQ 60

Query: 61  FQPQIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFP-QQQQQQQQQQ 120
           FQPQIQ QQQFQQQQ SPQFQQ QPSPQ QPQQQ S QFQQQ+QPQHFP QQQQQQQQQQ
Sbjct: 61  FQPQIQSQQQFQQQQLSPQFQQQQPSPQTQPQQQPSPQFQQQLQPQHFPQQQQQQQQQQQ 120

Query: 121 QYHFQQQQQRLLQQQQQQQQQQKQQQYHQHQSLASHFHLFHFVEKLADAIETGVRDQQSD 180
           QYHFQQQQQ   QQQQQQQQQQ+QQQYHQHQSLASHFHLFH VEKLAD IE G RDQQSD
Sbjct: 121 QYHFQQQQQ---QQQQQQQQQQQQQQYHQHQSLASHFHLFHLVEKLADTIENGARDQQSD 180

Query: 181 ALVNDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRR 226
           ALVNDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLL++RR
Sbjct: 181 ALVNDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLNHRR 225

[Close]

BLAST of Carg04310 vs. ExPASy TrEMBL
Match: A0A6J1D301 (Mediator of RNA polymerase II transcription subunit 9 OS=Momordica charantia OX=3673 GN=LOC111016860 PE=3 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 1.2e-76
Identity = 197/231 (85.28%), Postives = 204/231 (88.31%), Query Frame = 0


Query: 1   MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSLQQQQQQQQQLSFHHHPQQFQP 60
           MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSLQQQQ       FHH  QQFQP
Sbjct: 1   MDFSGGGNWSMIPNIPTLSNSSNLSNQESLYLQQQQFSLQQQQ------PFHHQQQQFQP 60

Query: 61  QIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFPQQQQQQQQQQQYHF 120
           Q+QP QQFQQQQ SPQFQQ QPSPQIQPQQ  S QFQQQ+QPQHFP   QQQQQQQQYHF
Sbjct: 61  QLQP-QQFQQQQPSPQFQQQQPSPQIQPQQP-SPQFQQQLQPQHFP---QQQQQQQQYHF 120

Query: 121 ----QQQQQRLL--QQQQQQQQQQKQQQYHQHQSLASHFHLFHFVEKLADAIETGVRDQQ 180
               QQQQQRLL  QQQQQQQQQQ+QQQYHQHQSLASHFHLFH VEKLADAIE G RDQQ
Sbjct: 121 QQQQQQQQQRLLQQQQQQQQQQQQQQQQYHQHQSLASHFHLFHLVEKLADAIENGARDQQ 180

Query: 181 SDALVNDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRR 226
           SDALVNDLNNHFEKCQQLLNSISGSLSSKAMTV+GQKKKLEEH+QLL++RR
Sbjct: 181 SDALVNDLNNHFEKCQQLLNSISGSLSSKAMTVDGQKKKLEEHKQLLNHRR 220

[Close]

BLAST of Carg04310 vs. ExPASy TrEMBL
Match: A0A6J1HX54 (Mediator of RNA polymerase II transcription subunit 9 OS=Cucurbita maxima OX=3661 GN=LOC111467667 PE=3 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 1.8e-69
Identity = 183/234 (78.21%), Postives = 186/234 (79.49%), Query Frame = 0


Query: 1   MDFSGGGNWSMIPNIPTL---------SNSSNLSNQESLYLQQQQFSLQQQQQQQQQLSF 60
           MDFS GGNWSMIPNIP L         SNSSNLSNQESLYLQQQQFSLQQQQQQQQQ SF
Sbjct: 1   MDFS-GGNWSMIPNIPNLSNSSNLSNHSNSSNLSNQESLYLQQQQFSLQQQQQQQQQQSF 60

Query: 61  HHHPQQFQPQIQPQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFPQQQQQ 120
           H   QQFQPQIQ QQQFQQQQ SPQFQQ QPSP          QFQQQ+QPQHFP  QQQ
Sbjct: 61  HQQQQQFQPQIQSQQQFQQQQLSPQFQQQQPSP----------QFQQQLQPQHFP--QQQ 120

Query: 121 QQQQQQYHFQQQQQRLLQQQQQQQQQQKQQQYHQHQSLASHFHLFHFVEKLADAIETGVR 180
           QQQQQQYHFQQQQQ              QQQYHQHQSLASHFHLFH VEKLAD IE G R
Sbjct: 121 QQQQQQYHFQQQQQ--------------QQQYHQHQSLASHFHLFHLVEKLADTIENGAR 180

Query: 181 DQQSDALVNDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRR 226
           DQQSDALVNDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLL++RR
Sbjct: 181 DQQSDALVNDLNNHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLNHRR 207

[Close]

BLAST of Carg04310 vs. TAIR 10
Match: AT1G55080.1 (MED9; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G29580.1); Has 67203 Blast hits to 25757 proteins in 1293 species: Archae - 12; Bacteria - 4374; Metazoa - 24340; Fungi - 7940; Plants - 5927; Viruses - 273; Other Eukaryotes - 24337 (source: NCBI BLink). )

HSP 1 Score: 132.9 bits (333), Expect = 4.4e-31
Identity = 127/237 (53.59%), Postives = 142/237 (59.92%), Query Frame = 0


Query: 5   GGGNWSMIPNIPTLSNSSNLSN-QESLYLQQQQFSLQQQQQQQQQLSFHHHPQQFQPQIQ 64
           GGGNWSMIPN+    N    +N  + L+LQQQQ  LQQQQQQQQQ  FH   QQ   Q Q
Sbjct: 7   GGGNWSMIPNVQAQGNFGTPTNHDQQLFLQQQQ--LQQQQQQQQQQQFHLQQQQQTQQQQ 66

Query: 65  PQQQFQQQQSSPQFQQLQPSPQIQPQQQLSTQFQQQMQPQHFPQQQQQQQQQQQYHFQQQ 124
            Q Q QQQQ   Q+QQ Q       QQQ   Q +    P   PQ  Q    QQ      Q
Sbjct: 67  QQFQPQQQQEMQQYQQFQQQQHFIQQQQFQQQQRLLQSPPLQPQSLQSPPPQQTMVHTPQ 126

Query: 125 QQRLLQQQQQQQQQQKQQQYHQHQSLASHFHLFHFVEKLADAIETGVRDQQSDALVNDLN 184
                 QQQQQ  Q   Q   QHQSLASHFHL+  VEKLAD IE G RDQ SDALVN+LN
Sbjct: 127 SMMHTPQQQQQLVQTPVQTPQQHQSLASHFHLYPMVEKLADVIENGTRDQNSDALVNELN 186

Query: 185 NHFEKCQQLLNSISGSLSSKAMTVEGQKKKLEEHEQLLSYRRAASIAQIDRTLDIVT 241
           +HF+KCQQLLNSISGSL SK MTV+GQK+ +EE EQLL  RR   +       +IVT
Sbjct: 187 SHFDKCQQLLNSISGSLGSKTMTVDGQKRNVEESEQLLQQRRDLIVEYRKSIEEIVT 241

The following BLAST results are available for this feature:

BLAST of Carg04310 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7036431.1	2.1e-144	100.00	Mediator of RNA polymerase II transcription subunit 9, partial [Cucurbita argyro...	[more]
KAG6606716.1	6.9e-95	98.23	hypothetical protein SDJN03_00058, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023523773.1	5.8e-94	97.35	mediator of RNA polymerase II transcription subunit 9-like [Cucurbita pepo subsp...	[more]
XP_022949500.1	1.4e-92	96.92	mediator of RNA polymerase II transcription subunit 9-like [Cucurbita moschata]	[more]
XP_022997827.1	4.2e-92	96.05	mediator of RNA polymerase II transcription subunit 9-like [Cucurbita maxima]	[more]

BLAST of Carg04310 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RWA2	6.2e-30	53.59	Mediator of RNA polymerase II transcription subunit 9 OS=Arabidopsis thaliana OX...	[more]
Q55F68	5.8e-04	42.72	Adenylate cyclase, terminal-differentiation specific OS=Dictyostelium discoideum...	[more]

BLAST of Carg04310 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GCY0	7.0e-93	96.92	mediator of RNA polymerase II transcription subunit 9-like OS=Cucurbita moschata...	[more]
A0A6J1K651	2.0e-92	96.05	mediator of RNA polymerase II transcription subunit 9-like OS=Cucurbita maxima O...	[more]
A0A6J1FZC2	1.2e-79	88.65	mediator of RNA polymerase II transcription subunit 9-like OS=Cucurbita moschata...	[more]
A0A6J1D301	1.2e-76	85.28	Mediator of RNA polymerase II transcription subunit 9 OS=Momordica charantia OX=...	[more]
A0A6J1HX54	1.8e-69	78.21	Mediator of RNA polymerase II transcription subunit 9 OS=Cucurbita maxima OX=366...	[more]

BLAST of Carg04310 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G55080.1	4.4e-31	53.59	MED9; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G2958...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 120..141
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 44..114
None	No IPR available	PANTHER	PTHR37188:SF1	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT-RELATED	coord: 121..226
IPR038790	Mediator of RNA polymerase II transcription subunit 9, plant	PANTHER	PTHR37188	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT-RELATED	coord: 121..226

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg04310-RA	Carg04310-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
cellular_component	GO:0016592	mediator complex
molecular_function	GO:0003712	transcription coregulator activity