Carg04251 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04251
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	2-oxoglutarate-dependent dioxygenase AOP3-like
Location	Carg_Chr01: 544245 .. 545881 (-)
RNA-Seq Expression	Carg04251
Synteny	Carg04251

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGTTTCGTTCTTCCACAAAGCCTGAGGTTCCAGTCATCAACTTCTCCATTGAAAACCTTCGTCCTAAAAGCACCAAATGGGTAGAAACATGCACCCATGTGAGGGCTGCTCTTGAAGAATATGGGTGTTTCTTGGCTGAGTATGAGAATGATGTTGAGCAAGAGGTTGAGCTTGATGATGCCATTTTCAATGGCTTGGAAAAGCTTTTTGATTTGCCTTTACAAACTAAAATGAAGAATGTTTCTGATAAGCCTTACCATGGCTTTTTGGACCACAGGGCTCCCTTTGTTCTTCCTCTTCATGAAAGCTTTGGCATTGAAAATGCAGCGAGTTTTGAAGGTGTTGAGTCCTTTGTGAAGCTCATGTGGCCTTCTGGAAATCATCATTTCTGGTAAACTTTGTATGAATTACATGGTTGTCTATTTTGGGTGTAAATCTTTAAAATCTTACTTGGAAATGATTTTGTTGGACAGTCAGGATTTGTTTGCATACACAAAGATGGTATCAGAGATGGAGAAGTTAGTGAAGAGGATGGTGTTTGAAAGCTATGGAGTGGGAAAGTACTATGATTCTCATATTGCTTCAACCACTTATTTGCTTAGAGGCATGAAATATAGAATCCCTGAGATGGGTGAACAGAATATTGGAGCAGAAGCTCACACAGACAAGAGTTTCTTCACTATTCTTCATCAAAATGGAGTCAATGGCTTGGAAATCAAAGCAAAAAAGGATGATCGTTGGATTAGACTTCAATTCAATCCAAATTCCTTCTTGGTCATGGCTGGAGATGCTTGTTTGGTATACCTCTTACTTGCACTTTCATCCATACTTCAATTATACACACACATATACGGTAATAGTCCAAGTCCACCGCTAACAGATATTGTTCTGTTTGGACTTCCCCTCAAGATTTTTAAAATGCTTATGTTAGACAGAGGTTTCCACACTCGATGCGGGATCTCACAATCCACTCCCCTTCGGGGCCAACGTTCTTGCTGACACTCGTTCCCTTCTCTAATCGATGTGGGACCCCCAATCCACCCTCTTTGGGGTCCAACGTCCTTCCTAACACATCACCCGATGCTTAGCTTTGATATCATTTGTAACAGCCCAAACTCACTACTAGTAGATATTGTTTTCTTTAGGCTTTCCCTAACTCACTACTAGTAGATATTGCCTTCTTTAGGCTTTCCCTTTTGGTCTTCTCCTCAAAGTTTTTAAAGACTCGTCTACTGGGGAGAGATTTCCACACCCTTATAAAGAATGATTCGTTTTCTTCCTAACGTGAGATCTCACATGTAGTTATCAATTGTTTATCCCAAAGATTGATGATTTGAATACTTGAATTAAGTATTGAATGGTAAGAACAAAATTTGATTTTGATGGATAGGCATGGAGCAATGGGAGAATCCACTCAGCCACACATAGAGTTATAATAGAGGGCAGCAAGGAAAGATATTCAACAGCCCTATTTTCATACCACAAAGGAGTTATTGAGATTCCAGATGAGTTGGTGGACGAACAATTCCCCTTGAAATTCAACCCTTTTAACCATTATGGATTACTCGGTTATTTCTCCAAAGAAGAGTGCGAGAAAACGCCTTCCACTGCCGGAGCTTATTGTGGAGTTTGA

mRNA sequence

ATGGGGTTTCGTTCTTCCACAAAGCCTGAGGTTCCAGTCATCAACTTCTCCATTGAAAACCTTCGTCCTAAAAGCACCAAATGGGTAGAAACATGCACCCATGTGAGGGCTGCTCTTGAAGAATATGGGTGTTTCTTGGCTGAGTATGAGAATGATGTTGAGCAAGAGGTTGAGCTTGATGATGCCATTTTCAATGGCTTGGAAAAGCTTTTTGATTTGCCTTTACAAACTAAAATGAAGAATGTTTCTGATAAGCCTTACCATGGCTTTTTGGACCACAGGGCTCCCTTTGTTCTTCCTCTTCATGAAAGCTTTGGCATTGAAAATGCAGCGAGTTTTGAAGGTGTTGAGTCCTTTGTGAAGCTCATTCAGGATTTGTTTGCATACACAAAGATGGTATCAGAGATGGAGAAGTTAGTGAAGAGGATGGTGTTTGAAAGCTATGGAGTGGGAAAGTACTATGATTCTCATATTGCTTCAACCACTTATTTGCTTAGAGGCATGAAATATAGAATCCCTGAGATGGGTGAACAGAATATTGGAGCAGAAGCTCACACAGACAAGAGTTTCTTCACTATTCTTCATCAAAATGGAGCATGGAGCAATGGGAGAATCCACTCAGCCACACATAGAGTTATAATAGAGGGCAGCAAGGAAAGATATTCAACAGCCCTATTTTCATACCACAAAGGAGTTATTGAGATTCCAGATGAGTTGGTGGACGAACAATTCCCCTTGAAATTCAACCCTTTTAACCATTATGGATTACTCGGTTATTTCTCCAAAGAAGAGTGCGAGAAAACGCCTTCCACTGCCGGAGCTTATTGTGGAGTTTGA

Coding sequence (CDS)

ATGGGGTTTCGTTCTTCCACAAAGCCTGAGGTTCCAGTCATCAACTTCTCCATTGAAAACCTTCGTCCTAAAAGCACCAAATGGGTAGAAACATGCACCCATGTGAGGGCTGCTCTTGAAGAATATGGGTGTTTCTTGGCTGAGTATGAGAATGATGTTGAGCAAGAGGTTGAGCTTGATGATGCCATTTTCAATGGCTTGGAAAAGCTTTTTGATTTGCCTTTACAAACTAAAATGAAGAATGTTTCTGATAAGCCTTACCATGGCTTTTTGGACCACAGGGCTCCCTTTGTTCTTCCTCTTCATGAAAGCTTTGGCATTGAAAATGCAGCGAGTTTTGAAGGTGTTGAGTCCTTTGTGAAGCTCATTCAGGATTTGTTTGCATACACAAAGATGGTATCAGAGATGGAGAAGTTAGTGAAGAGGATGGTGTTTGAAAGCTATGGAGTGGGAAAGTACTATGATTCTCATATTGCTTCAACCACTTATTTGCTTAGAGGCATGAAATATAGAATCCCTGAGATGGGTGAACAGAATATTGGAGCAGAAGCTCACACAGACAAGAGTTTCTTCACTATTCTTCATCAAAATGGAGCATGGAGCAATGGGAGAATCCACTCAGCCACACATAGAGTTATAATAGAGGGCAGCAAGGAAAGATATTCAACAGCCCTATTTTCATACCACAAAGGAGTTATTGAGATTCCAGATGAGTTGGTGGACGAACAATTCCCCTTGAAATTCAACCCTTTTAACCATTATGGATTACTCGGTTATTTCTCCAAAGAAGAGTGCGAGAAAACGCCTTCCACTGCCGGAGCTTATTGTGGAGTTTGA

Protein sequence

MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQNIGAEAHTDKSFFTILHQNGAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECEKTPSTAGAYCGV

Homology

[Close]

BLAST of Carg04251 vs. NCBI nr
Match: KAG7036488.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 577.8 bits (1488), Expect = 5.1e-161
Identity = 278/278 (100.00%), Postives = 278/278 (100.00%), Query Frame = 0


Query: 1   MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60
           MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD
Sbjct: 1   MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60

Query: 61  DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120
           DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV
Sbjct: 61  DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120

Query: 121 KLIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQNI 180
           KLIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQNI
Sbjct: 121 KLIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQNI 180

Query: 181 GAEAHTDKSFFTILHQNGAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELV 240
           GAEAHTDKSFFTILHQNGAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELV
Sbjct: 181 GAEAHTDKSFFTILHQNGAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELV 240

Query: 241 DEQFPLKFNPFNHYGLLGYFSKEECEKTPSTAGAYCGV 279
           DEQFPLKFNPFNHYGLLGYFSKEECEKTPSTAGAYCGV
Sbjct: 241 DEQFPLKFNPFNHYGLLGYFSKEECEKTPSTAGAYCGV 278

[Close]

BLAST of Carg04251 vs. NCBI nr
Match: KAG6606775.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 552.0 bits (1421), Expect = 3.0e-153
Identity = 277/320 (86.56%), Postives = 278/320 (86.88%), Query Frame = 0


Query: 1   MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60
           MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD
Sbjct: 1   MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60

Query: 61  DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120
           DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV
Sbjct: 61  DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120

Query: 121 KLI---------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYR 180
           KL+         QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYR
Sbjct: 121 KLMWPSGNHHFCQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYR 180

Query: 181 IPEMGEQNIGAEAHTDKSFFTILHQNG--------------------------------- 240
           IPEMGEQNIGAEAHTDKSFFTILHQNG                                 
Sbjct: 181 IPEMGEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDRWIRLQFNPNSFLVMAGDACL 240

Query: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 279
           AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG
Sbjct: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 300

[Close]

BLAST of Carg04251 vs. NCBI nr
Match: XP_022948698.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata])

HSP 1 Score: 546.2 bits (1406), Expect = 1.6e-151
Identity = 275/320 (85.94%), Postives = 276/320 (86.25%), Query Frame = 0


Query: 1   MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60
           MGFRSSTKPEVPVINFSIENL PKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD
Sbjct: 1   MGFRSSTKPEVPVINFSIENLGPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60

Query: 61  DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120
           DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV
Sbjct: 61  DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120

Query: 121 KLI---------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYR 180
           KL+         QDLFAYTKMVSEMEKLVKRMVFESYGV KYYDSHIASTTYLLRGMKYR
Sbjct: 121 KLMWPSGNHHFCQDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYR 180

Query: 181 IPEMGEQNIGAEAHTDKSFFTILHQNG--------------------------------- 240
           IPEMGEQNIGAEAHTDKSFFTILHQNG                                 
Sbjct: 181 IPEMGEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNSNSFLVMAGDACL 240

Query: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 279
           AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG
Sbjct: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 300

[Close]

BLAST of Carg04251 vs. NCBI nr
Match: XP_023525673.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 538.1 bits (1385), Expect = 4.5e-149
Identity = 269/320 (84.06%), Postives = 276/320 (86.25%), Query Frame = 0


Query: 1   MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60
           MGFRSST PEVPVI+FSIENLRPKSTKWVETC+HVRAALEEYGCFLAEYENDVEQEVELD
Sbjct: 1   MGFRSSTNPEVPVISFSIENLRPKSTKWVETCSHVRAALEEYGCFLAEYENDVEQEVELD 60

Query: 61  DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120
           DAIFNGLEKLFDLPL+TKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV
Sbjct: 61  DAIFNGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120

Query: 121 KLI---------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYR 180
           KL+         +DLFAYTKMVSEMEKLVKRMVFESYGV KYYDSHIASTTYLLRGMKYR
Sbjct: 121 KLMWPSGNHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYR 180

Query: 181 IPEMGEQNIGAEAHTDKSFFTILHQNG--------------------------------- 240
           IPEMGEQNIGAEAHTDKSFFTILHQNG                                 
Sbjct: 181 IPEMGEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNPNSFLVMAGDACL 240

Query: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 279
           AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIP+ELVDEQFPLKFNPFNHYGLLG
Sbjct: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPNELVDEQFPLKFNPFNHYGLLG 300

[Close]

BLAST of Carg04251 vs. NCBI nr
Match: XP_022997882.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita maxima])

HSP 1 Score: 522.3 bits (1344), Expect = 2.5e-144
Identity = 264/320 (82.50%), Postives = 270/320 (84.38%), Query Frame = 0


Query: 1   MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60
           MGFRSS KPEVP INFSIENLRPKSTKWVETC+HVRAALEEYGCFLAE EN+    VELD
Sbjct: 1   MGFRSSKKPEVPAINFSIENLRPKSTKWVETCSHVRAALEEYGCFLAECENN----VELD 60

Query: 61  DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120
           DAIFNGLEKLFDLPL+TKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV
Sbjct: 61  DAIFNGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120

Query: 121 KLI---------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYR 180
           KL+         +DLFAYTKMVSEMEKLVKRMVFESYGV KYYDSHIASTTYLLRGMKYR
Sbjct: 121 KLMWPSGNHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYR 180

Query: 181 IPEMGEQNIGAEAHTDKSFFTILHQNG--------------------------------- 240
           IPEMGEQNIGAEAHTDKSFFTILHQNG                                 
Sbjct: 181 IPEMGEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNPNSFLVMAGDACL 240

Query: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 279
           AWSNGRIHSATHRV+IEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG
Sbjct: 241 AWSNGRIHSATHRVLIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 300

[Close]

BLAST of Carg04251 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.2e-48
Identity = 113/304 (37.17%), Postives = 164/304 (53.95%), Query Frame = 0


Query: 10  EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69
           ++PVI+FS +NL+P S+KW E    V  ALE+YGCF A ++   +  VEL+ ++F  +E 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFD---KLSVELNRSVFEAMED 72

Query: 70  LFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVK-------- 129
           LF+LP+ TK +NVS KP+HG+L H       L+ES GI +A   E V  F +        
Sbjct: 73  LFELPIPTKQRNVSSKPFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWPDHGN 132

Query: 130 --LIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIP------ 189
             + + +  +++ + E++ +V+RM+ ES+G+  Y D H+ ST YL R MKY  P      
Sbjct: 133 KSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDD 192

Query: 190 EMGEQNIGAEAHTDKSFFTILHQ-----------NGAW---------------------S 249
           +  E  +G  +HTDK+  TILHQ           +  W                      
Sbjct: 193 DDEETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALL 252

Query: 250 NGRIHSATHRVIIEGSKERYSTALFSYHKG--VIEIPDELVDEQFPLKFNPFNHYGLLGY 264
           NGR+HS  HRVI+ G K RYST LFS  K   +I+ P+ELVD++ P  F PF +   L +
Sbjct: 253 NGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 307

[Close]

BLAST of Carg04251 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 2.1e-48
Identity = 113/303 (37.29%), Postives = 165/303 (54.46%), Query Frame = 0


Query: 10  EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69
           ++PVI+FS +NL+P S+KW E    V  ALE+YGCF A ++   +  VEL+ ++F  +E 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFD---KLSVELNRSVFEAMED 72

Query: 70  LFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVK-------- 129
           LF+LP+ TK +NVS KP+HG+L H       L+ES GI++A   E V  F +        
Sbjct: 73  LFELPIPTKQRNVSSKPFHGYLCHN------LYESLGIDDANVLEKVNDFTQQLWPDHGN 132

Query: 130 --LIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIP-----E 189
             + + +  +++ + E++ +V+RM+ ES+G+  Y D H+ ST YL R MKY  P     +
Sbjct: 133 KSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDD 192

Query: 190 MGEQNIGAEAHTDKSFFTILHQ-----------NGAW---------------------SN 249
             E  +G  +HTDK+  TILHQ           +  W                      N
Sbjct: 193 DEETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALLN 252

Query: 250 GRIHSATHRVIIEGSKERYSTALFSYHKG--VIEIPDELVDEQFPLKFNPFNHYGLLGYF 264
           GR+HS  HRVI+ G K RYST LFS  K   +I+ P+ELVD++ P  F PF +   L +F
Sbjct: 253 GRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFF 306

[Close]

BLAST of Carg04251 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 2.7e-48
Identity = 113/302 (37.42%), Postives = 164/302 (54.30%), Query Frame = 0


Query: 10  EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69
           ++PVI+FS +NL+P S+KW E    V  ALE+YGCF A ++   +  VEL+ ++F  +E 
Sbjct: 6   QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFD---KLSVELNRSVFEAMED 65

Query: 70  LFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVK-------- 129
           LF+LP+ TK +NVS K +HG+L H       L+ES GI +A   E V  F +        
Sbjct: 66  LFELPIPTKQRNVSSKLFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWPDHGN 125

Query: 130 --LIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIP----EM 189
             + + +  +++ + E++ +V+RM+ ES+G+ KY D H+ ST YL R MKY  P    + 
Sbjct: 126 KSISETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDD 185

Query: 190 GEQNIGAEAHTDKSFFTILHQ-----------NGAW---------------------SNG 249
            E  +G  +HTDK+  TILHQ           +  W                      NG
Sbjct: 186 EETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALLNG 245

Query: 250 RIHSATHRVIIEGSKERYSTALFSYHKG--VIEIPDELVDEQFPLKFNPFNHYGLLGYFS 264
           R+HS  HRVI+ G K RYST LFS  K   +I+ P+ELVD++ P  F PF +   L +F 
Sbjct: 246 RLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQ 298

[Close]

BLAST of Carg04251 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 2.8e-45
Identity = 113/299 (37.79%), Postives = 158/299 (52.84%), Query Frame = 0


Query: 6   STKP-EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIF 65
           ST P  +PVI+ S++NL+P +T W    T VR ALEEYGCF A  +       EL  A+ 
Sbjct: 3   STAPLRLPVIDLSMKNLKPGTTSWNSVRTQVREALEEYGCFEAVID---AVSPELQKAVC 62

Query: 66  NGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI- 125
           N   +L +LPL+TKM N +   Y GF       +  L+E  G+      E VE F  L+ 
Sbjct: 63  NKGHELLNLPLETKMLNGNKPEYDGFTS-----IPNLNEGMGVGRITDLEKVERFTNLMW 122

Query: 126 --------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEM 185
                   + +++Y K ++E++ ++K MVFES+G+ K++DS   ST YLL  M+Y+ P  
Sbjct: 123 PEGNKDFCETVYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCESTNYLLHFMRYQQPGK 182

Query: 186 GEQNIGAEAHTDKSFFTILHQNG------------------------------AWSNGRI 245
             ++     H DKS  TI++QN                               AWSNGR+
Sbjct: 183 DGRSPALSLHKDKSILTIVNQNDVKGLEFETKDGEWILPTADNHIVLLGDCFMAWSNGRL 242

Query: 246 HSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGY-FSKE 264
           HS  HRV +  ++ R ST+ FS+ K +IE P ELVDE+ PL FNPF    LL Y F+KE
Sbjct: 243 HSPLHRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCFTKE 293

[Close]

BLAST of Carg04251 vs. ExPASy Swiss-Prot
Match: Q945B4 (2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP3 PE=2 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.7e-34
Identity = 114/412 (27.67%), Postives = 170/412 (41.26%), Query Frame = 0


Query: 6   STKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFN 65
           S  P++P+I  S + L+P S+KWV+  + VR ALE+YGCF A+ +   +  +EL  ++  
Sbjct: 3   SCSPQLPLICLSDQTLKPGSSKWVKVRSDVRKALEDYGCFEAKID---QVSMELQGSVLK 62

Query: 66  GLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV----- 125
            +++LF LP + K +NV  KP+ G+  H       L ESFGI++A   E    F      
Sbjct: 63  AMQELFALPTEAKQRNVCPKPFAGYFSHNG-----LSESFGIKDANILEKAHEFTQQLWP 122

Query: 126 ---KLIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY------- 185
              K I+ +  Y + ++E++ +V+R++ ESYG+  + D H+ ST Y +R MKY       
Sbjct: 123 EGNKSIKMIQLYAEKLAELDMMVRRLILESYGIEYFIDEHLNSTYYRMRLMKYIARPDND 182

Query: 186 ---------------------RIPEMG--------------------------------- 245
                                 + + G                                 
Sbjct: 183 ITAAVGANVDNGANDNADGDANVNDDGASIGVKVNVDVGDDVNDNDSVNIGVGVDINVET 242

Query: 246 -------------------------------EQNIGAE----AHTDKSFFTILHQN---- 279
                                          E N+ AE    +HTDKS   I++Q+    
Sbjct: 243 NVNGDLDAEANGDATAWVVGAVSGNASVGAKEANVDAELGLPSHTDKSLSGIIYQHQIDG 302

[Close]

BLAST of Carg04251 vs. ExPASy TrEMBL
Match: A0A6J1GA17 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111452291 PE=3 SV=1)

HSP 1 Score: 546.2 bits (1406), Expect = 7.9e-152
Identity = 275/320 (85.94%), Postives = 276/320 (86.25%), Query Frame = 0


Query: 1   MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60
           MGFRSSTKPEVPVINFSIENL PKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD
Sbjct: 1   MGFRSSTKPEVPVINFSIENLGPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60

Query: 61  DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120
           DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV
Sbjct: 61  DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120

Query: 121 KLI---------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYR 180
           KL+         QDLFAYTKMVSEMEKLVKRMVFESYGV KYYDSHIASTTYLLRGMKYR
Sbjct: 121 KLMWPSGNHHFCQDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYR 180

Query: 181 IPEMGEQNIGAEAHTDKSFFTILHQNG--------------------------------- 240
           IPEMGEQNIGAEAHTDKSFFTILHQNG                                 
Sbjct: 181 IPEMGEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNSNSFLVMAGDACL 240

Query: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 279
           AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG
Sbjct: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 300

[Close]

BLAST of Carg04251 vs. ExPASy TrEMBL
Match: A0A6J1K6A3 (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucurbita maxima OX=3661 GN=LOC111492707 PE=3 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 1.2e-144
Identity = 264/320 (82.50%), Postives = 270/320 (84.38%), Query Frame = 0


Query: 1   MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60
           MGFRSS KPEVP INFSIENLRPKSTKWVETC+HVRAALEEYGCFLAE EN+    VELD
Sbjct: 1   MGFRSSKKPEVPAINFSIENLRPKSTKWVETCSHVRAALEEYGCFLAECENN----VELD 60

Query: 61  DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120
           DAIFNGLEKLFDLPL+TKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV
Sbjct: 61  DAIFNGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120

Query: 121 KLI---------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYR 180
           KL+         +DLFAYTKMVSEMEKLVKRMVFESYGV KYYDSHIASTTYLLRGMKYR
Sbjct: 121 KLMWPSGNHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYR 180

Query: 181 IPEMGEQNIGAEAHTDKSFFTILHQNG--------------------------------- 240
           IPEMGEQNIGAEAHTDKSFFTILHQNG                                 
Sbjct: 181 IPEMGEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNPNSFLVMAGDACL 240

Query: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 279
           AWSNGRIHSATHRV+IEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG
Sbjct: 241 AWSNGRIHSATHRVLIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 300

[Close]

BLAST of Carg04251 vs. ExPASy TrEMBL
Match: A0A6J1DH70 (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Momordica charantia OX=3673 GN=LOC111021051 PE=3 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 1.7e-114
Identity = 212/315 (67.30%), Postives = 240/315 (76.19%), Query Frame = 0


Query: 5   SSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIF 64
           S  K ++PVIN SIEN+ P+ST W+E C+H+RAALEEYGCFL E+ +      ELDD IF
Sbjct: 6   SLAKAKLPVINLSIENVSPESTNWLEICSHLRAALEEYGCFLGEFGSATP---ELDDEIF 65

Query: 65  NGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI- 124
            GLE+LFDLPL+TKMKNVSDKPYHGFLDHR PFVLPLH+SFGIEN+ SFE V+SFV L+ 
Sbjct: 66  KGLEELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVNLMW 125

Query: 125 --------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEM 184
                   +++F+YTKMVSEMEKLVK MVFESYGV KYYDSHIASTTYLLR MKYRIPEM
Sbjct: 126 PSGNRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIPEM 185

Query: 185 GEQNIGAEAHTDKSFFTILHQNG--------------------------------AWSNG 244
           GE+NIGA+AHTDKSFFTILHQN                                 AWSNG
Sbjct: 186 GEENIGADAHTDKSFFTILHQNEVNGLEIKTKDGHWLGVEFTPNSFLVMAGDACLAWSNG 245

Query: 245 RIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKE 279
           RIHSATHRV+IEG+KERYSTALFSYHKG+IEIP ELVDE+ PL+F+PFNHYGLL YFS  
Sbjct: 246 RIHSATHRVMIEGTKERYSTALFSYHKGIIEIPYELVDEKHPLRFHPFNHYGLLAYFSTH 305

[Close]

BLAST of Carg04251 vs. ExPASy TrEMBL
Match: A0A1S4DWR1 (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucumis melo OX=3656 GN=LOC103490084 PE=3 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 2.3e-87
Identity = 166/243 (68.31%), Postives = 187/243 (76.95%), Query Frame = 0


Query: 79  MKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI---------QDLFAY 138
           MKNVSDKPYHGFLD RAPFVLPLH+S GIENA+S+E V+SFV L+         ++LFAY
Sbjct: 1   MKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQSFVNLLWPSGNHHFCENLFAY 60

Query: 139 TKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQNIGAEAHTDKS 198
           +K ++EME+LVKRMVFESYGVGKY DSHIASTTYLLR MKYRIP+MGE+NIGAEAHTDKS
Sbjct: 61  SKKITEMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKS 120

Query: 199 FFTILHQ----NG------------------------------AWSNGRIHSATHRVIIE 258
           FFTILHQ    NG                              AWSNGRIHSATHRVIIE
Sbjct: 121 FFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIE 180

Query: 259 GSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECEKTPSTAGAY 279
           G+KERYSTALFSYHKG+IEIP ELVD++ PL+F PFNHYGLLGYFS ++C+K PSTA AY
Sbjct: 181 GNKERYSTALFSYHKGIIEIPQELVDDKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAY 240

[Close]

BLAST of Carg04251 vs. ExPASy TrEMBL
Match: A0A6P3YXF4 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Ziziphus jujuba OX=326968 GN=LOC107406140 PE=3 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 5.2e-79
Identity = 156/313 (49.84%), Postives = 198/313 (63.26%), Query Frame = 0


Query: 10  EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69
           ++P I+FS ENL+P ++ W  T    R ALE+YGCF+A Y+   +   +L D+IF  L++
Sbjct: 8   KLPAIDFSKENLKPGTSCWSSTAIDARQALEQYGCFVAIYD---KMSPQLHDSIFRELKE 67

Query: 70  LFDLPLQTKMKNVSDKPYHGFL---DHRAPFVLPLHESFGIENAASFEGVESFVKLI--- 129
           LFDLP + KMKN+SDKPY G+        P V  LHES GIEN  + E V+SF  L+   
Sbjct: 68  LFDLPTEIKMKNISDKPYFGYAAGNSATTPHVPSLHESLGIENTTTLEAVQSFTNLMWPA 127

Query: 130 ------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGE 189
                 + + +YTK+V+E+E++VKRMVFESYG  KYYDSH+ STTYLLR +KYR PE+ E
Sbjct: 128 GNHSFCETVLSYTKLVAELEQMVKRMVFESYGAEKYYDSHVGSTTYLLRQIKYRAPEIDE 187

Query: 190 QNIGAEAHTDKSFFTILHQNG--------------------------------AWSNGRI 249
            ++G E HTDKSF TILHQN                                 AWSN RI
Sbjct: 188 SDVGCEIHTDKSFITILHQNEVEGFELKTKDGYWIGFEPSPSSFLVLAGDAFLAWSNDRI 247

Query: 250 HSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEEC 279
           HSA HRV+++GS+ER+S  +FSYH G+IEIP E+VDEQ PLKF  F HYGLL YF     
Sbjct: 248 HSAAHRVVMKGSRERFSVGVFSYHNGIIEIPVEVVDEQHPLKFKAFEHYGLLNYFYTAPG 307

[Close]

BLAST of Carg04251 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 236.9 bits (603), Expect = 2.0e-62
Identity = 125/311 (40.19%), Postives = 180/311 (57.88%), Query Frame = 0


Query: 9   PEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLE 68
           P++P ++FS E+L+P +  W  T  ++R ALEEYGCF+ + ++  +  ++L D +F  L 
Sbjct: 3   PKIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKD--KTPLDLLDRVFGSLV 62

Query: 69  KLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI----- 128
            LFDLP QTKMKN  DKP +G++  + P  LPLHES GI+NA S E   SF  L+     
Sbjct: 63  DLFDLPTQTKMKNKYDKPLNGYVG-QIP-ALPLHESLGIDNATSLEATRSFTGLMWPQGN 122

Query: 129 ----QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQN 188
               + L+ Y +  +E++++V RMVF+SY V KYYD +I STTYLLR +K R P      
Sbjct: 123 EHFSECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPT 182

Query: 189 IGAEAHTDKSFFTILHQ---NG------------------------------AWSNGRIH 248
           +G   HTDKSF TILHQ   NG                              AWSN R+ 
Sbjct: 183 LGFVTHTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDALMAWSNDRVW 242

Query: 249 SATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECE 278
           S  H+V++ G  +RYS  +F+++ G +++P+EL+D Q PL + PF+H GLL ++  +   
Sbjct: 243 SPRHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGY 302

[Close]

BLAST of Carg04251 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 221.5 bits (563), Expect = 8.6e-58
Identity = 116/311 (37.30%), Postives = 173/311 (55.63%), Query Frame = 0


Query: 10  EVPVINF-SIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLE 69
           +VP+++  S + L+P ++ W         ALEEYGCFLA Y+   +Q   LDD+IF   E
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQ---LDDSIFAAAE 68

Query: 70  KLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI----- 129
           +LFDLP +TK KNV++KPYHG++      V+PLHE  G++   + E  + F  L+     
Sbjct: 69  ELFDLPTETKKKNVNEKPYHGYVGQMP--VIPLHEGLGVDYVTNKEIAQRFTHLMWPQGN 128

Query: 130 ----QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQN 189
                 +  ++  V+E+++LV RM+FE+YGV K+Y+SH+ S TYLL+ +KY  P      
Sbjct: 129 DRFCNTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPESISM 188

Query: 190 IGAEAHTDKSFFTILHQNG--------------------------------AWSNGRIHS 249
                HTDK+F +ILHQN                                  WSN RI S
Sbjct: 189 PAFPQHTDKTFLSILHQNDVNGLEVKSKDGEWISLQLPPKSYVVMAGDISMGWSNDRIRS 248

Query: 250 ATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECEK 279
             HRV +EG K RY+  LFS+   ++ IP+ELVD++ PL + PF++  L+ +++ +E  +
Sbjct: 249 CEHRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGRE 308

[Close]

BLAST of Carg04251 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 212.6 bits (540), Expect = 4.0e-55
Identity = 119/322 (36.96%), Postives = 180/322 (55.90%), Query Frame = 0


Query: 1   MGFRSSTKP-EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVEL 60
           MG  +   P  +PVI+FS +NL+P   +W  T   V+ AL++YG F A ++       EL
Sbjct: 1   MGSETPLLPLRLPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFD---RIPFEL 60

Query: 61  DDAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESF 120
             ++F  LE+LFDLPLQTK++NVS KP+HG++      ++PL+ES GI+++   E V++F
Sbjct: 61  RKSVFGALEELFDLPLQTKLRNVSKKPFHGYVGQYP--MVPLYESMGIDDSDIAEKVDAF 120

Query: 121 ---------VKLIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY 180
                    +     + +++K +SE++  ++RM+ ES+G+ KY D H+ ST YLLR MKY
Sbjct: 121 TEKLWPQGNISFSTTIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKY 180

Query: 181 RIPEMGEQNIGAEAHTDKSFFTILHQNG-------------------------------- 240
           + P+  E  +G  AHTDK+  TIL+QN                                 
Sbjct: 181 KGPDTEETKVGLNAHTDKNIVTILYQNHVEGLEVQTKDKNWIKVKPTQDSFTVMIGDSLY 240

Query: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKG--VIEIPDELVDEQFPLKFNPFNHYGL 279
           A  NGR+HS  HRV++ G++ RYS  LFS  K   ++  PDELVDE+ P  F PF+H   
Sbjct: 241 ALLNGRLHSPYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEF 300

[Close]

BLAST of Carg04251 vs. TAIR 10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 194.9 bits (494), Expect = 8.6e-50
Identity = 113/304 (37.17%), Postives = 164/304 (53.95%), Query Frame = 0


Query: 10  EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69
           ++PVI+FS +NL+P S+KW E    V  ALE+YGCF A ++   +  VEL+ ++F  +E 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFD---KLSVELNRSVFEAMED 72

Query: 70  LFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVK-------- 129
           LF+LP+ TK +NVS KP+HG+L H       L+ES GI +A   E V  F +        
Sbjct: 73  LFELPIPTKQRNVSSKPFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWPDHGN 132

Query: 130 --LIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIP------ 189
             + + +  +++ + E++ +V+RM+ ES+G+  Y D H+ ST YL R MKY  P      
Sbjct: 133 KSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDD 192

Query: 190 EMGEQNIGAEAHTDKSFFTILHQ-----------NGAW---------------------S 249
           +  E  +G  +HTDK+  TILHQ           +  W                      
Sbjct: 193 DDEETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALL 252

Query: 250 NGRIHSATHRVIIEGSKERYSTALFSYHKG--VIEIPDELVDEQFPLKFNPFNHYGLLGY 264
           NGR+HS  HRVI+ G K RYST LFS  K   +I+ P+ELVD++ P  F PF +   L +
Sbjct: 253 NGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 307

[Close]

BLAST of Carg04251 vs. TAIR 10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 189.9 bits (481), Expect = 2.8e-48
Identity = 113/316 (35.76%), Postives = 163/316 (51.58%), Query Frame = 0


Query: 10  EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69
           E+P+IN S +NL+  +  W  T   VR A+E +G F+AEY N       L  +I    ++
Sbjct: 8   ELPIINLSDKNLKQNTELWSFTRDSVREAMEHHGWFVAEYNN---FPTGLHQSILEAAKE 67

Query: 70  LFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI------ 129
           L DLP++ K+KN + K  HG++   +    P+HE  GI+     +    F +L+      
Sbjct: 68  LLDLPVEIKLKNENHKAGHGYITMMSD-GQPVHEGLGIDQVNDVQQCRGFSRLMWPDDHD 127

Query: 130 ------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGE 189
                 + + AY KM +E+E+LV RM+FESY V KY + +I  T YLLR +KYR    GE
Sbjct: 128 DNDRFCETVHAYAKMQAELEQLVIRMLFESYNVEKYTEKYIGGTRYLLRLLKYRRLPNGE 187

Query: 190 QNIGAEAHTDKSFFTILHQNG---------------------------------AWSNGR 249
            N    +HTDKSF +ILHQN                                  AWSN R
Sbjct: 188 PNRKFISHTDKSFISILHQNHITGLMLKSEKEDVWYHFTPSPTRFVVIAGDAIMAWSNDR 247

Query: 250 IHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEE 279
           I +  H+V +E  + RYS   FS+ +G+I  P+E+VD+  PL +NPF+H GLL Y+   E
Sbjct: 248 IKACYHKVEMESVEMRYSLGFFSFQEGMISTPEEMVDKDHPLAYNPFHHDGLLEYYETLE 307

The following BLAST results are available for this feature:

BLAST of Carg04251 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7036488.1	5.1e-161	100.00	putative 2-oxoglutarate-dependent dioxygenase AOP1, partial [Cucurbita argyrospe...	[more]
KAG6606775.1	3.0e-153	86.56	putative 2-oxoglutarate-dependent dioxygenase AOP1, partial [Cucurbita argyrospe...	[more]
XP_022948698.1	1.6e-151	85.94	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata]	[more]
XP_023525673.1	4.5e-149	84.06	2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita pepo subsp. pepo]	[more]
XP_022997882.1	2.5e-144	82.50	2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita maxima]	[more]

BLAST of Carg04251 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZTA3	1.2e-48	37.17	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37...	[more]
Q945B6	2.1e-48	37.29	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=...	[more]
Q944Z5	2.7e-48	37.42	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]
A0A0N9HQ36	2.8e-45	37.79	Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=...	[more]
Q945B4	1.7e-34	27.67	2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]

BLAST of Carg04251 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GA17	7.9e-152	85.94	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1K6A3	1.2e-144	82.50	2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1DH70	1.7e-114	67.30	2-oxoglutarate-dependent dioxygenase AOP3-like OS=Momordica charantia OX=3673 GN...	[more]
A0A1S4DWR1	2.3e-87	68.31	2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A6P3YXF4	5.2e-79	49.84	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Ziziphus jujuba OX=326968 ...	[more]

BLAST of Carg04251 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52790.1	2.0e-62	40.19	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52800.1	8.6e-58	37.30	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52820.1	4.0e-55	36.96	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G03070.1	8.6e-50	37.17	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G15540.1	2.8e-48	35.76	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 199..257 e-value: 2.6E-8 score: 35.8 coord: 2..198 e-value: 9.1E-22 score: 80.0
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 11..90 e-value: 5.4E-7 score: 30.4
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 199..277
None	No IPR available	PANTHER	PTHR47990:SF17	2-OXOGLUTARATE-DEPENDENT DIOXYGENASE AOP3-LIKE	coord: 199..277
None	No IPR available	PANTHER	PTHR47990:SF17	2-OXOGLUTARATE-DEPENDENT DIOXYGENASE AOP3-LIKE	coord: 5..197
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 5..197
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 8..261

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg04251-RA	Carg04251-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding