Carg04147 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04147
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	sodium/potassium/calcium exchanger 1-like
Location	Carg_Chr19: 7915594 .. 7916112 (-)
RNA-Seq Expression	Carg04147
Synteny	Carg04147

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGTTGGTGATTTCAGCTTCTGTGCTCTCTGCAATGGTGTTTTACTCATCTCTCCTCCCTTTCCTCCACTCTTTGAACCTTTATATCTCTGCAACAATCCACAAAAACTACATGTTCTTGCTCTGCAATGGCCTCCTTGTCTTCATCGTCCGAAACTCCGGTCTTATCAGTAGCTCTGGCCGCGAACAGTGCGGCGGACATGGCAGCAGAGAAGCTGAATCTCCAGGGGCTGCAATGGAGGATTCCACCACAATAATTGAGAGAAAAGTTGAAGATGGAGAAGAAAGGGAAAATGGGTTTATTGCAGAAGAAGAACAAGAAGAAAGTGAAAGATTGGTTACAGAAGAAGAACAACAACAAGAACACGAAGAACAAGAAGAACAAGAAGAAGAAGAAGAAGAAGCAGAAGAAGTTGGGGCTTTGAGCAATGAAGAATTGGACAAGAAATTTGAAGAATTTATCAGAAAAATGAAGGCAGGAATCAAACTTGAATCACAGCAAGTGCTTATTTTGTAA

mRNA sequence

ATGAAGTTGGTGATTTCAGCTTCTGTGCTCTCTGCAATGGTGTTTTACTCATCTCTCCTCCCTTTCCTCCACTCTTTGAACCTTTATATCTCTGCAACAATCCACAAAAACTACATGTTCTTGCTCTGCAATGGCCTCCTTGTCTTCATCGTCCGAAACTCCGGTCTTATCAGTAGCTCTGGCCGCGAACAGTGCGGCGGACATGGCAGCAGAGAAGCTGAATCTCCAGGGGCTGCAATGGAGGATTCCACCACAATAATTGAGAGAAAAGTTGAAGATGGAGAAGAAAGGGAAAATGGGTTTATTGCAGAAGAAGAACAAGAAGAAAGTGAAAGATTGGTTACAGAAGAAGAACAACAACAAGAACACGAAGAACAAGAAGAACAAGAAGAAGAAGAAGAAGAAGCAGAAGAAGTTGGGGCTTTGAGCAATGAAGAATTGGACAAGAAATTTGAAGAATTTATCAGAAAAATGAAGGCAGGAATCAAACTTGAATCACAGCAAGTGCTTATTTTGTAA

Coding sequence (CDS)

ATGAAGTTGGTGATTTCAGCTTCTGTGCTCTCTGCAATGGTGTTTTACTCATCTCTCCTCCCTTTCCTCCACTCTTTGAACCTTTATATCTCTGCAACAATCCACAAAAACTACATGTTCTTGCTCTGCAATGGCCTCCTTGTCTTCATCGTCCGAAACTCCGGTCTTATCAGTAGCTCTGGCCGCGAACAGTGCGGCGGACATGGCAGCAGAGAAGCTGAATCTCCAGGGGCTGCAATGGAGGATTCCACCACAATAATTGAGAGAAAAGTTGAAGATGGAGAAGAAAGGGAAAATGGGTTTATTGCAGAAGAAGAACAAGAAGAAAGTGAAAGATTGGTTACAGAAGAAGAACAACAACAAGAACACGAAGAACAAGAAGAACAAGAAGAAGAAGAAGAAGAAGCAGAAGAAGTTGGGGCTTTGAGCAATGAAGAATTGGACAAGAAATTTGAAGAATTTATCAGAAAAATGAAGGCAGGAATCAAACTTGAATCACAGCAAGTGCTTATTTTGTAA

Protein sequence

MKLVISASVLSAMVFYSSLLPFLHSLNLYISATIHKNYMFLLCNGLLVFIVRNSGLISSSGREQCGGHGSREAESPGAAMEDSTTIIERKVEDGEERENGFIAEEEQEESERLVTEEEQQQEHEEQEEQEEEEEEAEEVGALSNEELDKKFEEFIRKMKAGIKLESQQVLIL

Homology

[Close]

BLAST of Carg04147 vs. NCBI nr
Match: KAG7011863.1 (hypothetical protein SDJN02_26769, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 291.2 bits (744), Expect = 5.9e-75
Identity = 172/172 (100.00%), Postives = 172/172 (100.00%), Query Frame = 0


Query: 1   MKLVISASVLSAMVFYSSLLPFLHSLNLYISATIHKNYMFLLCNGLLVFIVRNSGLISSS 60
           MKLVISASVLSAMVFYSSLLPFLHSLNLYISATIHKNYMFLLCNGLLVFIVRNSGLISSS
Sbjct: 1   MKLVISASVLSAMVFYSSLLPFLHSLNLYISATIHKNYMFLLCNGLLVFIVRNSGLISSS 60

Query: 61  GREQCGGHGSREAESPGAAMEDSTTIIERKVEDGEERENGFIAEEEQEESERLVTEEEQQ 120
           GREQCGGHGSREAESPGAAMEDSTTIIERKVEDGEERENGFIAEEEQEESERLVTEEEQQ
Sbjct: 61  GREQCGGHGSREAESPGAAMEDSTTIIERKVEDGEERENGFIAEEEQEESERLVTEEEQQ 120

Query: 121 QEHEEQEEQEEEEEEAEEVGALSNEELDKKFEEFIRKMKAGIKLESQQVLIL 173
           QEHEEQEEQEEEEEEAEEVGALSNEELDKKFEEFIRKMKAGIKLESQQVLIL
Sbjct: 121 QEHEEQEEQEEEEEEAEEVGALSNEELDKKFEEFIRKMKAGIKLESQQVLIL 172

[Close]

BLAST of Carg04147 vs. NCBI nr
Match: KAG6572225.1 (hypothetical protein SDJN03_28953, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 285.0 bits (728), Expect = 4.2e-73
Identity = 172/177 (97.18%), Postives = 172/177 (97.18%), Query Frame = 0


Query: 1   MKLVISASVLSAMVFYSSLLPFLHSLNLYISATIHKNYMFLLCNGLLVFIVRNSGLISSS 60
           MKLVISASVLSAMVFYSSLLPFLHSLNLYISATIHKNYMFLLCNGLLVFIVRNSGLISSS
Sbjct: 1   MKLVISASVLSAMVFYSSLLPFLHSLNLYISATIHKNYMFLLCNGLLVFIVRNSGLISSS 60

Query: 61  GREQCGGHGSREAESPGAAMEDSTTIIERKVEDGEERENGFIAEEEQEESERLVTEEEQQ 120
           GREQCGGHGSREAESPGAAMEDSTTIIERKVEDGEERENGFIAEEEQEESERLVTEEEQQ
Sbjct: 61  GREQCGGHGSREAESPGAAMEDSTTIIERKVEDGEERENGFIAEEEQEESERLVTEEEQQ 120

Query: 121 QEHEEQEEQ-----EEEEEEAEEVGALSNEELDKKFEEFIRKMKAGIKLESQQVLIL 173
           QEHEEQEEQ     EEEEEEAEEVGALSNEELDKKFEEFIRKMKAGIKLESQQVLIL
Sbjct: 121 QEHEEQEEQEEEEEEEEEEEAEEVGALSNEELDKKFEEFIRKMKAGIKLESQQVLIL 177

[Close]

BLAST of Carg04147 vs. NCBI nr
Match: XP_022969273.1 (sodium/potassium/calcium exchanger 1-like [Cucurbita maxima])

HSP 1 Score: 270.0 bits (689), Expect = 1.4e-68
Identity = 165/172 (95.93%), Postives = 167/172 (97.09%), Query Frame = 0


Query: 1   MKLVISASVLSAMVFYSSLLPFLHSLNLYISATIHKNYMFLLCNGLLVFIVRNSGLISSS 60
           MKLVISASVLSAMVFYSSLLPFLHSLNLYISATIHKNYMFLLCNGLLVFIVRNSGLISSS
Sbjct: 1   MKLVISASVLSAMVFYSSLLPFLHSLNLYISATIHKNYMFLLCNGLLVFIVRNSGLISSS 60

Query: 61  GREQCGGHGSREAESPGAAMEDSTTIIERKVEDGEERENGFIAEEEQEESERLVTEEEQQ 120
           GREQCGGHGSREAE+ GAAMEDSTTIIERKVEDGEERENGFIAEEEQ ESERLVTEEEQQ
Sbjct: 61  GREQCGGHGSREAEALGAAMEDSTTIIERKVEDGEERENGFIAEEEQ-ESERLVTEEEQQ 120

Query: 121 QEHEEQEEQEEEEEEAEEVGALSNEELDKKFEEFIRKMKAGIKLESQQVLIL 173
           QEHE  EEQEEE+ EAEEVGALSNEELDKKFEEFIRKMKAGIKLESQQVLIL
Sbjct: 121 QEHE--EEQEEEKAEAEEVGALSNEELDKKFEEFIRKMKAGIKLESQQVLIL 169

[Close]

BLAST of Carg04147 vs. NCBI nr
Match: XP_023530308.1 (uncharacterized protein LOC111792915 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 173.7 bits (439), Expect = 1.4e-39
Identity = 115/172 (66.86%), Postives = 132/172 (76.74%), Query Frame = 0


Query: 1   MKLVISASVLSAMVFYSSLLPFLHSLNLYISATIHKNYMFLLCNGLLVFIVRNSGLISSS 60
           MKL+ISAS+LSAMVFYSSLLPFLHS NLYISAT HKNYMFLLCNGLLVFIVRNSGLIS S
Sbjct: 1   MKLLISASLLSAMVFYSSLLPFLHSFNLYISATAHKNYMFLLCNGLLVFIVRNSGLISCS 60

Query: 61  GREQCGGHGSREAESPGAAMEDSTTIIERKVEDGEERENGFIAEEEQEESERLVTEEEQQ 120
           G    GG  SR AE+  AAM D ++ IE KVED EER NG I  ++ EESER + E    
Sbjct: 61  GDRHSGG-DSRGAEALEAAMADGSSKIESKVEDREERGNGLI--QKDEESERSIVE---- 120

Query: 121 QEHEEQEEQEEEEEEAEEVGALSNEELDKKFEEFIRKMKAGIKLESQQVLIL 173
                    ++++++ EEVGALS EEL+KK EEFIRKMKAGIKLESQQ+L+L
Sbjct: 121 ---------DDDDDDDEEVGALSKEELNKKCEEFIRKMKAGIKLESQQLLVL 156

[Close]

BLAST of Carg04147 vs. NCBI nr
Match: XP_022928452.1 (uncharacterized protein LOC111435260 [Cucurbita moschata])

HSP 1 Score: 171.8 bits (434), Expect = 5.2e-39
Identity = 114/172 (66.28%), Postives = 133/172 (77.33%), Query Frame = 0


Query: 1   MKLVISASVLSAMVFYSSLLPFLHSLNLYISATIHKNYMFLLCNGLLVFIVRNSGLISSS 60
           MKL+ISAS+LSAMVFYSSLLPFLHS NLYISAT HKNYMFLLCNGLLVFIVRNSGLIS S
Sbjct: 1   MKLLISASLLSAMVFYSSLLPFLHSFNLYISATAHKNYMFLLCNGLLVFIVRNSGLISCS 60

Query: 61  GREQCGGHGSREAESPGAAMEDSTTIIERKVEDGEERENGFIAEEEQEESERLVTEEEQQ 120
           G    GG  SR AE+  A+M D ++ IE KVED EER NG I  ++ EESER + E    
Sbjct: 61  GDRHSGG-DSRGAEALEASMADGSSKIESKVEDREERGNGLI--QKDEESERSIVE---- 120

Query: 121 QEHEEQEEQEEEEEEAEEVGALSNEELDKKFEEFIRKMKAGIKLESQQVLIL 173
                 ++  +++++ EEVGALS EEL+KK EEFIRKMKAGIKLESQQ+L+L
Sbjct: 121 ------DDDNDDDDDEEEVGALSKEELNKKCEEFIRKMKAGIKLESQQLLVL 159

[Close]

BLAST of Carg04147 vs. ExPASy TrEMBL
Match: A0A6J1I0H9 (sodium/potassium/calcium exchanger 1-like OS=Cucurbita maxima OX=3661 GN=LOC111468328 PE=4 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 6.8e-69
Identity = 165/172 (95.93%), Postives = 167/172 (97.09%), Query Frame = 0


Query: 1   MKLVISASVLSAMVFYSSLLPFLHSLNLYISATIHKNYMFLLCNGLLVFIVRNSGLISSS 60
           MKLVISASVLSAMVFYSSLLPFLHSLNLYISATIHKNYMFLLCNGLLVFIVRNSGLISSS
Sbjct: 1   MKLVISASVLSAMVFYSSLLPFLHSLNLYISATIHKNYMFLLCNGLLVFIVRNSGLISSS 60

Query: 61  GREQCGGHGSREAESPGAAMEDSTTIIERKVEDGEERENGFIAEEEQEESERLVTEEEQQ 120
           GREQCGGHGSREAE+ GAAMEDSTTIIERKVEDGEERENGFIAEEEQ ESERLVTEEEQQ
Sbjct: 61  GREQCGGHGSREAEALGAAMEDSTTIIERKVEDGEERENGFIAEEEQ-ESERLVTEEEQQ 120

Query: 121 QEHEEQEEQEEEEEEAEEVGALSNEELDKKFEEFIRKMKAGIKLESQQVLIL 173
           QEHE  EEQEEE+ EAEEVGALSNEELDKKFEEFIRKMKAGIKLESQQVLIL
Sbjct: 121 QEHE--EEQEEEKAEAEEVGALSNEELDKKFEEFIRKMKAGIKLESQQVLIL 169

[Close]

BLAST of Carg04147 vs. ExPASy TrEMBL
Match: A0A6J1EJY9 (uncharacterized protein LOC111435260 OS=Cucurbita moschata OX=3662 GN=LOC111435260 PE=4 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 2.5e-39
Identity = 114/172 (66.28%), Postives = 133/172 (77.33%), Query Frame = 0


Query: 1   MKLVISASVLSAMVFYSSLLPFLHSLNLYISATIHKNYMFLLCNGLLVFIVRNSGLISSS 60
           MKL+ISAS+LSAMVFYSSLLPFLHS NLYISAT HKNYMFLLCNGLLVFIVRNSGLIS S
Sbjct: 1   MKLLISASLLSAMVFYSSLLPFLHSFNLYISATAHKNYMFLLCNGLLVFIVRNSGLISCS 60

Query: 61  GREQCGGHGSREAESPGAAMEDSTTIIERKVEDGEERENGFIAEEEQEESERLVTEEEQQ 120
           G    GG  SR AE+  A+M D ++ IE KVED EER NG I  ++ EESER + E    
Sbjct: 61  GDRHSGG-DSRGAEALEASMADGSSKIESKVEDREERGNGLI--QKDEESERSIVE---- 120

Query: 121 QEHEEQEEQEEEEEEAEEVGALSNEELDKKFEEFIRKMKAGIKLESQQVLIL 173
                 ++  +++++ EEVGALS EEL+KK EEFIRKMKAGIKLESQQ+L+L
Sbjct: 121 ------DDDNDDDDDEEEVGALSKEELNKKCEEFIRKMKAGIKLESQQLLVL 159

[Close]

BLAST of Carg04147 vs. ExPASy TrEMBL
Match: A0A6J1C4P4 (ATP-dependent RNA helicase MAK5 OS=Momordica charantia OX=3673 GN=LOC111007892 PE=4 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 2.8e-38
Identity = 115/171 (67.25%), Postives = 130/171 (76.02%), Query Frame = 0


Query: 1   MKLVISASVLSAMVFYSSLLPFLHSLNLYISATIHKNYMFLLCNGLLVFIVRNSGLISSS 60
           MK++ISAS+LSAMV YSSLLPFLHSLNLYISATIHKNY+FLLCNGLLVFIVRNSGLI  S
Sbjct: 1   MKILISASLLSAMVLYSSLLPFLHSLNLYISATIHKNYIFLLCNGLLVFIVRNSGLIGGS 60

Query: 61  GREQCGGHGSREAESPGAAMEDSTTIIERKVEDGEERENGFIAEEEQEESERLVTEEEQQ 120
           G EQ GG G                 +E KVEDGEERENG +A  EQEESE+L+TE+E  
Sbjct: 61  GEEQ-GGGGK----------------VESKVEDGEERENGLVA--EQEESEKLITEDE-- 120

Query: 121 QEHEEQEEQEEEEEEAEEVGALSNEELDKKFEEFIRKMKAGIKLESQQVLI 172
            EHEE+EE+EEE+      G LSNEEL++K EEFIRKMKA IK E QQVL+
Sbjct: 121 -EHEEEEEEEEED------GGLSNEELNQKCEEFIRKMKAEIKFELQQVLV 143

[Close]

BLAST of Carg04147 vs. ExPASy TrEMBL
Match: A0A0A0K349 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G032240 PE=4 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 1.1e-31
Identity = 108/173 (62.43%), Postives = 126/173 (72.83%), Query Frame = 0


Query: 1   MKLVISASVLSAMVFYSSLLPFLHSLNLYISATIHKNYMFLLCNGLLVFIVRNSGLISSS 60
           MKLVISAS+LSAMVFYSSLLPFLHSLNLYIS+TIHKNYMFLLCNGLLVFIVRNSGLI ++
Sbjct: 1   MKLVISASLLSAMVFYSSLLPFLHSLNLYISSTIHKNYMFLLCNGLLVFIVRNSGLIGNT 60

Query: 61  GREQCGGHGSREAESPGAA-MEDSTTIIERKVEDGEERENGFIAEEEQEESERLVTEEEQ 120
             E+  G+     E+ GAA M D    IE KVE  EER NG +  E  ++ E L+T+ E 
Sbjct: 61  SDERSSGN-----EALGAATMADGNRKIESKVEIEEERGNGLVVNE--DKIENLITDNEV 120

Query: 121 QQEHEEQEEQEEEEEEAEEVGALSNEELDKKFEEFIRKMKAGIKLESQQVLIL 173
           ++E     E+EEEEE         +EEL+KK EEFIRKMKAGIK ESQQ+ IL
Sbjct: 121 EEERFMSYEEEEEEE--------IDEELNKKCEEFIRKMKAGIKFESQQLFIL 158

[Close]

BLAST of Carg04147 vs. ExPASy TrEMBL
Match: A0A1S3CG16 (gem-associated protein 2-like OS=Cucumis melo OX=3656 GN=LOC103500515 PE=4 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 1.5e-28
Identity = 101/166 (60.84%), Postives = 117/166 (70.48%), Query Frame = 0


Query: 13  MVFYSSLLPFLHSLNLYISATIHKNYMFLLCNGLLVFIVRNSGLISSSGREQCGGHGSRE 72
           MVFYSSLLPFLHSLNLYIS+TIHKNYMFLLCNGLLVFIVRNSGLI ++    C    SR 
Sbjct: 1   MVFYSSLLPFLHSLNLYISSTIHKNYMFLLCNGLLVFIVRNSGLIGNT----CNEQSSRN 60

Query: 73  AESPGAAMEDSTTIIERK--VEDGEERENGFIAEEEQEESERLVTEEEQQQE----HEEQ 132
                  M D    IE K  VE  EER NG +  E  E+ E L+T+ E ++E    +EE+
Sbjct: 61  EALGAVTMADGNRKIESKVEVEVEEERRNGLVVNE--EKFENLITDNEDEEERFISYEEE 120

Query: 133 EEQEEEEEEAEEVGALSNEELDKKFEEFIRKMKAGIKLESQQVLIL 173
           EE+EEEEEE EE   + +EEL+KK EEFIRKMKAGIK ESQQ+ IL
Sbjct: 121 EEEEEEEEEEEEEEEI-DEELNKKCEEFIRKMKAGIKFESQQLFIL 159

The following BLAST results are available for this feature:

BLAST of Carg04147 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7011863.1	5.9e-75	100.00	hypothetical protein SDJN02_26769, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6572225.1	4.2e-73	97.18	hypothetical protein SDJN03_28953, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022969273.1	1.4e-68	95.93	sodium/potassium/calcium exchanger 1-like [Cucurbita maxima]	[more]
XP_023530308.1	1.4e-39	66.86	uncharacterized protein LOC111792915 [Cucurbita pepo subsp. pepo]	[more]
XP_022928452.1	5.2e-39	66.28	uncharacterized protein LOC111435260 [Cucurbita moschata]	[more]

BLAST of Carg04147 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg04147 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I0H9	6.8e-69	95.93	sodium/potassium/calcium exchanger 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1EJY9	2.5e-39	66.28	uncharacterized protein LOC111435260 OS=Cucurbita moschata OX=3662 GN=LOC1114352...	[more]
A0A6J1C4P4	2.8e-38	67.25	ATP-dependent RNA helicase MAK5 OS=Momordica charantia OX=3673 GN=LOC111007892 P...	[more]
A0A0A0K349	1.1e-31	62.43	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G032240 PE=4 SV=1	[more]
A0A1S3CG16	1.5e-28	60.84	gem-associated protein 2-like OS=Cucumis melo OX=3656 GN=LOC103500515 PE=4 SV=1	[more]

BLAST of Carg04147 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 103..145
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 59..147
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 98..113
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 120..141
None	No IPR available	PANTHER	PTHR34947:SF3	TRANSMEMBRANE PROTEIN	coord: 1..170
None	No IPR available	PANTHER	PTHR34947	TRANSMEMBRANE PROTEIN	coord: 1..170

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg04147-RA	Carg04147-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane