Carg04039 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg04039
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPfkB domain-containing protein
LocationCarg_Chr12: 2754911 .. 2757319 (-)
RNA-Seq ExpressionCarg04039
SyntenyCarg04039
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAGCCGAGCCTTTGCTTCCCACTGCCAATGAGTCTCAACCCGCCGCCCCTCTTATTCTTGGTCTTCAACCCGCTGCCCTCATCGACCATGTTGCCCGTGTCGATCCCTCTCTTCTCGATCGAATTCCCGGCGACCGCGGTGGCTCAATGCCTGTAAACTTACTTTTTTTTATTTCTATCGCTCAATTACGTCTTCTGTTCTGGAATCCTTGATTTTAAGATTAGGATTGTATTGACTCGTTTCTGTGTTTTTGATTGATCTTTGATCGGTGCGAGCGGATTCTGCTTCTGGCGTTGTTTTTGTTTCTTTACCCTATTGAATCGTTTTATCCTTATCGTTATTCTGTGTGATTTTAGAAGTGATTTTATAGTGTTCTGTGCAATTGGATTTCGAGCGGTGGTGTTGTTTTTGTTCTTCATTTTTATGAGTTTGGCTGGTTGGTGTTCTTGTGATTTTATGTTCTACTCGTAATTTGTGCTGGATTTCGTCTGTTTGAGTTTCTTTTCATGCATTCTGTTCGTTGTTTATATATATGTATATGGATTTAGGTGGGAATGGAAGAGCTTGAGAATATACTGAGGGAGGTGAAATCATATATCCTTTCCTCGCCTGATGTGCTGACTCCTTTCAAGACAATAGCGGGGGGAAGTGTTGCCAACACAATTCGAGGATTGAGTGCGGGGTTTGGAGTCAGCTGTGGAATTATTGGCGCCTATGGCGATGATCAGCAAGGAAAATTGTTTGTCTGTAACATGAGCTCCAATGGGGTCAATCTCTCAAGGTTGAGGATGATTAAAGGTCCCACAGCTCAGGTCTGCTTCTCCTTTAATGGAGGGGTATCCAAATTTGCATTCTGTGTTTATGTATTTTAAAGTTCTGAGAATGTATTGTGAATTATTCGGCAGTGTGTTTGCCTGGTCGATGCAATGGGAAATCGCACAATGCGACCCTCTCTCTCAAGTGCAGTCAAAGTTCAGGTATATTATCTCTTCGATTCGGAGTTCGTGTTCTTCGTTCGTGCGTGATTCTATATGCGTTTTGGATATAAACAGGTTAACTTAGATGGCTATCTGTTTTGGCATCTTTCAGGCAAATGAACTGACTAGAGATGACTTCAAAGGAGCTAAGGTTAGCATACTCACATCTTTATGTTCTTGTATAGCGGCCAGGATCGAAAATGGATCACGAACATATTATTCATTTTAAGAACTTCCTTTTCTGCCTTCACCCTCATATTTATGTTCTTTGATCATCATGTCATGTCATATCTTTTCAACTGATATCATTCTATTGAATCAATTGCCACACTTGAAAGATTCTTGTCTTGTCTTGCCATGTCTTTGTTGCAGTGGCTTGTAATGAGATATAGTATATTTAACATGGAAGTTATTGAAGCGGCTGTAAAGATGGCCAAACAAGAGAAAGTTTTTGTATCCTTGGATTTGGCCAGTTTCGAGGTATATTCCTTGACGACGTGAGCTTGCTTTTAGTTTCGAAGAAGAAGTTGTTACTTGTTCGATAAAGAATCTCACGTGTCGATGTTTGGCCAGATGGTTCGGGATTTTAGATCGCCTCTTCTCCAGCTACTAGAGTCTGGGGATATAGACCTTTGCTTTGCGAATGAGGACGAAGCAAAAGAGTTGATAGGGTGATGATAGCCATTGCTTTCAAACTTGGCTACTTTAGCTCTAGGGTGATGGCCTTGATTTCTGATACTATATTTAATCTTTACAGTGGTGAGAAAGATGCTGATCCTGAAGTTGCTCTAGAATTTCTTGCGAAGCACTGTCAATGGGCTGTCGTGACGTTAGGCGCCAATGGTTGTATCGCTAAGCACGGAAAAGAGGTTAGAGATTCACCTTTCACCTTTTGTGTCACATGTGATGATCTTGAAAAACATTAGATTACCTTCTCATCTCATGAAGTGATGAAAACAATCAAAACCAGTTTCTTAAGCCTACACATGGATATGTGAACCCTTAATGTTTGCACACTTTCTTGCACAATAGAACAGGAAAGTTTCTCGATGCCTGCATCGAGCGAATCCTCTGTTTTTTCCTATACTGGTTTTGATTTCTAGTTTGGCCCGAAACGACATACAAGGTCAAGAGATCACTGTGAAATCTAAGTGTTTGTGAATGCATATGGACTTTCAGATTGTCCGAGTTCCAGCAATAGGGGAATCAAAGGCGACCGATGCCACCGGAGCAGGCGACCTGTTTGCGAGCGGATTTTTGTACGGATTAGTGAAGGGATTGTCTCTGGAGAAATGCTGTCAATTGGGGTCGTGCAGCGGTGGTTCTGTTATCCGTTCACTCGGGGGTGAGGTCACCCCAGAGAATTGGCAATGGATGCACAAACATTTGCAGATCAAAGGGCTTCCTGTCCAAGAGATCCCTCAATGA

mRNA sequence

ATGGGAGCCGAGCCTTTGCTTCCCACTGCCAATGAGTCTCAACCCGCCGCCCCTCTTATTCTTGGTCTTCAACCCGCTGCCCTCATCGACCATGTTGCCCGTGTCGATCCCTCTCTTCTCGATCGAATTCCCGGCGACCGCGGTGGCTCAATGCCTGTGGGAATGGAAGAGCTTGAGAATATACTGAGGGAGGTGAAATCATATATCCTTTCCTCGCCTGATGTGCTGACTCCTTTCAAGACAATAGCGGGGGGAAGTGTTGCCAACACAATTCGAGGATTGAGTGCGGGGTTTGGAGTCAGCTGTGGAATTATTGGCGCCTATGGCGATGATCAGCAAGGAAAATTGTTTGTCTGTAACATGAGCTCCAATGGGGTCAATCTCTCAAGGTTGAGGATGATTAAAGGTCCCACAGCTCAGTGTGTTTGCCTGGTCGATGCAATGGGAAATCGCACAATGCGACCCTCTCTCTCAAGTGCAGTCAAAGTTCAGGCAAATGAACTGACTAGAGATGACTTCAAAGGAGCTAAGTGGCTTGTAATGAGATATAGTATATTTAACATGGAAGTTATTGAAGCGGCTGTAAAGATGGCCAAACAAGAGAAAGTTTTTGTATCCTTGGATTTGGCCAGTTTCGAGATGGTTCGGGATTTTAGATCGCCTCTTCTCCAGCTACTAGAGTCTGGGGATATAGACCTTTGCTTTGCGAATGAGGACGAAGCAAAAGAGTTGATAGGTGGTGAGAAAGATGCTGATCCTGAAGTTGCTCTAGAATTTCTTGCGAAGCACTGTCAATGGGCTGTCGTGACGTTAGGCGCCAATGGTTGTATCGCTAAGCACGGAAAAGAGATTGTCCGAGTTCCAGCAATAGGGGAATCAAAGGCGACCGATGCCACCGGAGCAGGCGACCTGTTTGCGAGCGGATTTTTGTACGGATTAGTGAAGGGATTGTCTCTGGAGAAATGCTGTCAATTGGGGTCGTGCAGCGGTGGTTCTGTTATCCGTTCACTCGGGGGTGAGGTCACCCCAGAGAATTGGCAATGGATGCACAAACATTTGCAGATCAAAGGGCTTCCTGTCCAAGAGATCCCTCAATGA

Coding sequence (CDS)

ATGGGAGCCGAGCCTTTGCTTCCCACTGCCAATGAGTCTCAACCCGCCGCCCCTCTTATTCTTGGTCTTCAACCCGCTGCCCTCATCGACCATGTTGCCCGTGTCGATCCCTCTCTTCTCGATCGAATTCCCGGCGACCGCGGTGGCTCAATGCCTGTGGGAATGGAAGAGCTTGAGAATATACTGAGGGAGGTGAAATCATATATCCTTTCCTCGCCTGATGTGCTGACTCCTTTCAAGACAATAGCGGGGGGAAGTGTTGCCAACACAATTCGAGGATTGAGTGCGGGGTTTGGAGTCAGCTGTGGAATTATTGGCGCCTATGGCGATGATCAGCAAGGAAAATTGTTTGTCTGTAACATGAGCTCCAATGGGGTCAATCTCTCAAGGTTGAGGATGATTAAAGGTCCCACAGCTCAGTGTGTTTGCCTGGTCGATGCAATGGGAAATCGCACAATGCGACCCTCTCTCTCAAGTGCAGTCAAAGTTCAGGCAAATGAACTGACTAGAGATGACTTCAAAGGAGCTAAGTGGCTTGTAATGAGATATAGTATATTTAACATGGAAGTTATTGAAGCGGCTGTAAAGATGGCCAAACAAGAGAAAGTTTTTGTATCCTTGGATTTGGCCAGTTTCGAGATGGTTCGGGATTTTAGATCGCCTCTTCTCCAGCTACTAGAGTCTGGGGATATAGACCTTTGCTTTGCGAATGAGGACGAAGCAAAAGAGTTGATAGGTGGTGAGAAAGATGCTGATCCTGAAGTTGCTCTAGAATTTCTTGCGAAGCACTGTCAATGGGCTGTCGTGACGTTAGGCGCCAATGGTTGTATCGCTAAGCACGGAAAAGAGATTGTCCGAGTTCCAGCAATAGGGGAATCAAAGGCGACCGATGCCACCGGAGCAGGCGACCTGTTTGCGAGCGGATTTTTGTACGGATTAGTGAAGGGATTGTCTCTGGAGAAATGCTGTCAATTGGGGTCGTGCAGCGGTGGTTCTGTTATCCGTTCACTCGGGGGTGAGGTCACCCCAGAGAATTGGCAATGGATGCACAAACATTTGCAGATCAAAGGGCTTCCTGTCCAAGAGATCCCTCAATGA

Protein sequence

MGAEPLLPTANESQPAAPLILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELENILREVKSYILSSPDVLTPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCNMSSNGVNLSRLRMIKGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWLVMRYSIFNMEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANEDEAKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATGAGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPVQEIPQ
Homology
BLAST of Carg04039 vs. NCBI nr
Match: XP_022951670.1 (uncharacterized protein LOC111454413 [Cucurbita moschata] >KAG6585583.1 hypothetical protein SDJN03_18316, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020498.1 hypothetical protein SDJN02_17183, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 728.4 bits (1879), Expect = 3.0e-206
Identity = 365/365 (100.00%), Postives = 365/365 (100.00%), Query Frame = 0

Query: 1   MGAEPLLPTANESQPAAPLILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELEN 60
           MGAEPLLPTANESQPAAPLILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELEN
Sbjct: 1   MGAEPLLPTANESQPAAPLILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELEN 60

Query: 61  ILREVKSYILSSPDVLTPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCN 120
           ILREVKSYILSSPDVLTPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCN
Sbjct: 61  ILREVKSYILSSPDVLTPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCN 120

Query: 121 MSSNGVNLSRLRMIKGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWLV 180
           MSSNGVNLSRLRMIKGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWLV
Sbjct: 121 MSSNGVNLSRLRMIKGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWLV 180

Query: 181 MRYSIFNMEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANEDE 240
           MRYSIFNMEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANEDE
Sbjct: 181 MRYSIFNMEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANEDE 240

Query: 241 AKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATG 300
           AKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATG
Sbjct: 241 AKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATG 300

Query: 301 AGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPV 360
           AGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPV
Sbjct: 301 AGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPV 360

Query: 361 QEIPQ 366
           QEIPQ
Sbjct: 361 QEIPQ 365

BLAST of Carg04039 vs. NCBI nr
Match: XP_023002394.1 (uncharacterized protein LOC111496250 [Cucurbita maxima])

HSP 1 Score: 726.1 bits (1873), Expect = 1.5e-205
Identity = 363/365 (99.45%), Postives = 365/365 (100.00%), Query Frame = 0

Query: 1   MGAEPLLPTANESQPAAPLILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELEN 60
           MGAEPLLPTANES+PAAPLILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELEN
Sbjct: 1   MGAEPLLPTANESEPAAPLILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELEN 60

Query: 61  ILREVKSYILSSPDVLTPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCN 120
           ILREVKSYILSSPDVLTPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCN
Sbjct: 61  ILREVKSYILSSPDVLTPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCN 120

Query: 121 MSSNGVNLSRLRMIKGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWLV 180
           MSSNGVNLSRLRMIKGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWLV
Sbjct: 121 MSSNGVNLSRLRMIKGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWLV 180

Query: 181 MRYSIFNMEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANEDE 240
           MRYSIFNMEVIEAAVKMAKQ+KVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANEDE
Sbjct: 181 MRYSIFNMEVIEAAVKMAKQDKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANEDE 240

Query: 241 AKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATG 300
           AKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATG
Sbjct: 241 AKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATG 300

Query: 301 AGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPV 360
           AGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPV
Sbjct: 301 AGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPV 360

Query: 361 QEIPQ 366
           QEIPQ
Sbjct: 361 QEIPQ 365

BLAST of Carg04039 vs. NCBI nr
Match: XP_023538348.1 (uncharacterized protein LOC111799162 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 725.3 bits (1871), Expect = 2.6e-205
Identity = 364/365 (99.73%), Postives = 364/365 (99.73%), Query Frame = 0

Query: 1   MGAEPLLPTANESQPAAPLILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELEN 60
           MGAEPLLPTANESQPAAPLILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELEN
Sbjct: 1   MGAEPLLPTANESQPAAPLILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELEN 60

Query: 61  ILREVKSYILSSPDVLTPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCN 120
           ILREVKSYILSSPDVLT FKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCN
Sbjct: 61  ILREVKSYILSSPDVLTSFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCN 120

Query: 121 MSSNGVNLSRLRMIKGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWLV 180
           MSSNGVNLSRLRMIKGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWLV
Sbjct: 121 MSSNGVNLSRLRMIKGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWLV 180

Query: 181 MRYSIFNMEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANEDE 240
           MRYSIFNMEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANEDE
Sbjct: 181 MRYSIFNMEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANEDE 240

Query: 241 AKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATG 300
           AKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATG
Sbjct: 241 AKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATG 300

Query: 301 AGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPV 360
           AGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPV
Sbjct: 301 AGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPV 360

Query: 361 QEIPQ 366
           QEIPQ
Sbjct: 361 QEIPQ 365

BLAST of Carg04039 vs. NCBI nr
Match: XP_038886551.1 (uncharacterized sugar kinase slr0537 [Benincasa hispida])

HSP 1 Score: 676.4 bits (1744), Expect = 1.4e-190
Identity = 343/366 (93.72%), Postives = 353/366 (96.45%), Query Frame = 0

Query: 1   MGAEPLLPTANESQPAAPLILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELEN 60
           MGAEPLL TAN+S+ AAPLILGLQPAALIDHV+RVD SLL+RIPGDRGGSMPVGMEELEN
Sbjct: 1   MGAEPLLHTANDSE-AAPLILGLQPAALIDHVSRVDSSLLNRIPGDRGGSMPVGMEELEN 60

Query: 61  ILREVKSYILSSP-DVLTPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVC 120
           ILREVKSY LS+P D LTP KTIAGGSVANTIRGLSAGFGVSCGIIGAYGDD+QGKLFV 
Sbjct: 61  ILREVKSYTLSTPDDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVS 120

Query: 121 NMSSNGVNLSRLRMIKGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWL 180
           NMSSNGVNLSRLRMIKGPTAQCVCLVDA+GNRTMRP LSSAVKVQ NELTRDDFKG+KWL
Sbjct: 121 NMSSNGVNLSRLRMIKGPTAQCVCLVDALGNRTMRPCLSSAVKVQGNELTRDDFKGSKWL 180

Query: 181 VMRYSIFNMEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANED 240
           VMRYSIFNMEVIEAAVKMAKQEKVFVSLDLASFEMVRDFR PLLQLLESGDIDLCFANED
Sbjct: 181 VMRYSIFNMEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRLPLLQLLESGDIDLCFANED 240

Query: 241 EAKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDAT 300
           EAKELIGGE DADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDAT
Sbjct: 241 EAKELIGGETDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDAT 300

Query: 301 GAGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLP 360
           GAGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIR+LGGEVTPENWQWMHKHLQIKGLP
Sbjct: 301 GAGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLP 360

Query: 361 VQEIPQ 366
           + EIPQ
Sbjct: 361 LPEIPQ 365

BLAST of Carg04039 vs. NCBI nr
Match: XP_004152687.1 (uncharacterized protein LOC101214597 [Cucumis sativus] >KGN62617.1 hypothetical protein Csa_021844 [Cucumis sativus])

HSP 1 Score: 667.5 bits (1721), Expect = 6.4e-188
Identity = 339/366 (92.62%), Postives = 351/366 (95.90%), Query Frame = 0

Query: 1   MGAEPLLPTANESQPAAPLILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELEN 60
           MGAEPLL T N+S+ A+PLILGLQPAALIDHV+RVD SLLDRIPGDRGGSMPVGMEELEN
Sbjct: 1   MGAEPLLHTVNDSE-ASPLILGLQPAALIDHVSRVDSSLLDRIPGDRGGSMPVGMEELEN 60

Query: 61  ILREVKSYILSSPDVL-TPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVC 120
           ILREVKSYILSSPD L TP KTIAGGSVANTIRGLSAGFG+SCGIIGA GDD+QGKLFV 
Sbjct: 61  ILREVKSYILSSPDDLTTPVKTIAGGSVANTIRGLSAGFGISCGIIGACGDDEQGKLFVS 120

Query: 121 NMSSNGVNLSRLRMIKGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWL 180
           NMSS+GVNLSRLRMIKGPTAQCVCLVDA+GNRTMRPSLSSAVKVQ NELTRDDFKG+KWL
Sbjct: 121 NMSSHGVNLSRLRMIKGPTAQCVCLVDALGNRTMRPSLSSAVKVQGNELTRDDFKGSKWL 180

Query: 181 VMRYSIFNMEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANED 240
           VMRYSIFN+EVIEAAVKMAKQEKVFVSLDLASFEMVRDFR PLLQLLESGDIDLCFANED
Sbjct: 181 VMRYSIFNIEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRLPLLQLLESGDIDLCFANED 240

Query: 241 EAKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDAT 300
           EAKELI GEKDADPEVALEFLAKHCQWA VTLGANGCIAKHGKEIVRVPAIGESKATDAT
Sbjct: 241 EAKELIRGEKDADPEVALEFLAKHCQWAAVTLGANGCIAKHGKEIVRVPAIGESKATDAT 300

Query: 301 GAGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLP 360
           GAGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIR+LGGEVTPENWQWMHKHLQIKGLP
Sbjct: 301 GAGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLP 360

Query: 361 VQEIPQ 366
           + EI Q
Sbjct: 361 LPEIRQ 365

BLAST of Carg04039 vs. ExPASy Swiss-Prot
Match: Q55480 (Uncharacterized sugar kinase slr0537 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr0537 PE=3 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 5.0e-18
Identity = 88/334 (26.35%), Postives = 149/334 (44.61%), Query Frame = 0

Query: 27  ALIDHVARVDPSLLDRIPGDRGGSMPVGMEELENILREVKSYILSSPDVLTPFKTIAGGS 86
           AL+D    V P  L  +  D+G         +  ++ E +   L +       K  +GGS
Sbjct: 13  ALVDMEFEVTPEQLASLGIDKG---------VMTLVEEARENELIAQLAQQRGKQSSGGS 72

Query: 87  VANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCNMSSNGV--NLSRLRMIKGPTAQCVCL 146
            ANT+  L A  G +       G D+ G  ++ +++  G+  N       +G T +C+  
Sbjct: 73  AANTLVSL-AQLGGTGFYACKVGKDEAGAFYLQDLNDCGLDTNPHHETAGEGITGKCLVF 132

Query: 147 VDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWLVMRYSIFNMEVIEAAV----KMAKQ 206
           V    +RTM   L  +  +   E+     K +++L +   +      +AA      +A+Q
Sbjct: 133 VTPDADRTMNAFLGISGSLSVTEMDWSALKQSQYLYLEGYLVTSPSAKAACIEAKAIAEQ 192

Query: 207 EKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANEDEAKELIGGEKDADPEVALEFL 266
             V   L L+   M + F+  L ++L SG +DL FANE EA E+ G    +D   A+ + 
Sbjct: 193 SGVKTCLSLSDPNMAKFFQDGLKEMLGSG-VDLLFANEAEALEMAG---TSDLNQAIAYC 252

Query: 267 AKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATGAGDLFASGFLYGLVKGLSLE 326
               +   +T G  G +   G+ ++ +    + +  D  GAGD++A GFLYGL  G+  E
Sbjct: 253 KSIAKNFALTRGGAGSLIFDGENLLTI-GTPKVQPIDTVGAGDMYAGGFLYGLTHGMDYE 312

Query: 327 KCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQ 355
           K  QL S +   V+   G  +  E  Q + + +Q
Sbjct: 313 KAGQLASETAAKVVTCYGPRLDTEILQEILQSVQ 331

BLAST of Carg04039 vs. ExPASy Swiss-Prot
Match: Q5JDG9 (ADP-dependent ribose-1-phosphate kinase OS=Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) OX=69014 GN=TK2029 PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 5.5e-17
Identity = 75/256 (29.30%), Postives = 118/256 (46.09%), Query Frame = 0

Query: 84  GGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCNMSSNGVNLSRLRMIKGPTAQCVC 143
           GG+ ANTI  L A FG+  G IGA G+D  G++ +      GV+   + +++ P+   V 
Sbjct: 39  GGAAANTISWL-AHFGLKTGYIGAVGNDDVGEMHIKYFQGIGVDTGGIDVVEEPSGVAVA 98

Query: 144 LVDAMGNRTMR-PSLSSAVKVQANELTRDDFKGAKWLVMRYSIFNMEVIEAAVKMAKQEK 203
           +V     R ++ P  +   + +    +R  F       +  S    E+IE AV  A Q  
Sbjct: 99  MVAGDDKRIVKYPGANLRRRFKPEYASRAKF-------LHLSSNPPELIEEAVNFASQRG 158

Query: 204 VFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANEDEAKELIGGEKDADPEVALEFLAK 263
           + VSLD+          +PL + LES  +D    NEDE +   G     DP +       
Sbjct: 159 IKVSLDIG--------EAPLPRELES-KVDYLMMNEDEYRRKYG---SLDPSLC------ 218

Query: 264 HCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATGAGDLFASGFLYGLVKGLSLEKC 323
             +  VVTL   G + + G  +  V  +  +K  D+TGAGD F +G +YG++ G SL   
Sbjct: 219 RAKNLVVTLNGGGALVREGDNVFEVRGL-SAKVVDSTGAGDSFDAGVIYGVLNGWSLLDS 267

Query: 324 CQLGSCSGGSVIRSLG 339
            +LG       ++ +G
Sbjct: 279 AKLGMLLAYLTVQKVG 267

BLAST of Carg04039 vs. ExPASy Swiss-Prot
Match: P77493 (Uncharacterized sugar kinase YdjH OS=Escherichia coli (strain K12) OX=83333 GN=ydjH PE=1 SV=2)

HSP 1 Score: 79.7 bits (195), Expect = 7.5e-14
Identity = 74/287 (25.78%), Postives = 131/287 (45.64%), Query Frame = 0

Query: 78  PFKTIA---GGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCNMSSNGVNLSRLRM- 137
           P + IA   GG   N    +S   G    ++   G D  G+  + +     +++  L+  
Sbjct: 32  PLERIAMTTGGDAINEATIISR-LGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQD 91

Query: 138 IKGPTAQCVCLVDAMGNRTMRPSLSSAV-KVQANELTRDDFKGAKWLVMRYSIFNMEVIE 197
           +   T+  V LV   G RT   + + ++ K+  +++    F  AK L +  SIFN  +++
Sbjct: 92  VSIDTSINVGLVTEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLA-SIFNSPLLD 151

Query: 198 AAV-----KMAKQEKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANEDEAKELIGG 257
                     AK  ++ +  D+     + +    + + L    +D  F N  EAK L G 
Sbjct: 152 GKALTEIFTQAKARQMIICADMIK-PRLNETLDDICEALSY--VDYLFPNFAEAKLLTG- 211

Query: 258 EKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATGAGDLFAS 317
            K+   E+A  FLA   +  V+  G +GC  K G   ++VPA+    A D  GAGD FAS
Sbjct: 212 -KETLDEIADCFLACGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFAS 271

Query: 318 GFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQ 355
           GF+  L++G +L +C +  + +    + S+G     +N + + + L+
Sbjct: 272 GFIAALLEGKNLRECARFANATAAISVLSVGATTGVKNRKLVEQLLE 311

BLAST of Carg04039 vs. ExPASy Swiss-Prot
Match: D9TT10 (ATP-dependent 6-phosphofructokinase OS=Thermoanaerobacterium thermosaccharolyticum (strain ATCC 7956 / DSM 571 / NCIMB 9385 / NCA 3814 / NCTC 13789 / WDCM 00135 / 2032) OX=580327 GN=pfkB PE=1 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.4e-12
Identity = 68/273 (24.91%), Postives = 123/273 (45.05%), Query Frame = 0

Query: 79  FKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCNMSSNGVNLSRLRMIKGPT 138
           +K   GGS AN I   S   G++  I+ + G+D  GK  +  +  + + +  +R +   T
Sbjct: 41  YKKYFGGSPAN-IAINSKMLGINSIIVSSVGNDGLGKFLLKKLQEHHIEIKYVRQVDYST 100

Query: 139 AQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAK------WLVMRYSIFNMEVIE 198
           +  V +  +  + T      +   ++ ++  +   +  K      W + R    +   +E
Sbjct: 101 SM-VLVTKSKSSPTPIFYRDADYHIEYSDELKYLIENTKIVHFSSWPISRNP--SRSTVE 160

Query: 199 AAVKMAKQEKVFVSLDLASFEMV----RDFRSPLLQLLESGDIDLCFANEDEAKELIGGE 258
             +   K+  V V  D     M+     D R  +  L+    +D+   +ED+A+ + G  
Sbjct: 161 ILIDECKKYDVLVCYDPNYHSMIWERGHDGREYIKSLI--AKVDIIKPSEDDAERIFG-- 220

Query: 259 KDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATGAGDLFASG 318
           KD       +FL    +  ++TLG +G I  +G+E +R   + + +  D TGAGD F SG
Sbjct: 221 KDTPENQLKKFLDLGAKLVILTLGKDGAIVSNGEETIRFNTLAD-EVVDTTGAGDAFWSG 280

Query: 319 FLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEV 342
           F  GL+KG +L+K  +LG       +R +G  V
Sbjct: 281 FYSGLIKGYTLKKSLELGFAVSAYKLRYVGAIV 304

BLAST of Carg04039 vs. ExPASy Swiss-Prot
Match: A4IPB3 (5-dehydro-2-deoxygluconokinase OS=Geobacillus thermodenitrificans (strain NG80-2) OX=420246 GN=iolC PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.6e-11
Identity = 65/270 (24.07%), Postives = 115/270 (42.59%), Query Frame = 0

Query: 79  FKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCNMSSNGVNLSRLRMIKGPT 138
           F    GGS AN   G+ A  G+  G IG   DDQ G+  V  + +NG++ S +   K  +
Sbjct: 39  FTKYVGGSPANIAIGM-ARLGMKTGFIGRVADDQMGRFIVQYLKNNGIDTSHVITDKSGS 98

Query: 139 AQCVCLVDAMG----NRTMRPSLSSAVKVQANELTRDDFKGAKWLVMRYSIF----NMEV 198
              +   +       +  M     + +K++ N++  D  + AK L++  +      + E 
Sbjct: 99  VTGLAFTEIKSPTDCSILMYRDNVADLKLEPNDIHEDYIRQAKCLLISGTALAKSPSREA 158

Query: 199 IEAAVKMAKQE--KVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANEDE--AKELIG 258
           +  A++ A++    VF  LD   +       + +   L +   D+     +E    E   
Sbjct: 159 VFLALEYARRHGVVVFFDLDYRPYTWQSKEETAIYYNLAAEKCDVIIGTREEFDMMEQFA 218

Query: 259 GEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATGAGDLFA 318
             +  D + A ++   H +  V+  G +G IA        V  I  +      GAGD +A
Sbjct: 219 VHQHDDEKTAQKWFDYHAKIVVIKHGKDGSIAYTKTGETFVGTIFPANIVKTFGAGDSYA 278

Query: 319 SGFLYGLVKGLSLEKCCQLGSCSGGSVIRS 337
           +GF+YGL+ G  + K  + G+ +   VI S
Sbjct: 279 AGFIYGLMNGWPIPKAMEYGAAAASIVISS 307

BLAST of Carg04039 vs. ExPASy TrEMBL
Match: A0A6J1GJH7 (uncharacterized protein LOC111454413 OS=Cucurbita moschata OX=3662 GN=LOC111454413 PE=4 SV=1)

HSP 1 Score: 728.4 bits (1879), Expect = 1.5e-206
Identity = 365/365 (100.00%), Postives = 365/365 (100.00%), Query Frame = 0

Query: 1   MGAEPLLPTANESQPAAPLILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELEN 60
           MGAEPLLPTANESQPAAPLILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELEN
Sbjct: 1   MGAEPLLPTANESQPAAPLILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELEN 60

Query: 61  ILREVKSYILSSPDVLTPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCN 120
           ILREVKSYILSSPDVLTPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCN
Sbjct: 61  ILREVKSYILSSPDVLTPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCN 120

Query: 121 MSSNGVNLSRLRMIKGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWLV 180
           MSSNGVNLSRLRMIKGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWLV
Sbjct: 121 MSSNGVNLSRLRMIKGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWLV 180

Query: 181 MRYSIFNMEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANEDE 240
           MRYSIFNMEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANEDE
Sbjct: 181 MRYSIFNMEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANEDE 240

Query: 241 AKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATG 300
           AKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATG
Sbjct: 241 AKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATG 300

Query: 301 AGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPV 360
           AGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPV
Sbjct: 301 AGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPV 360

Query: 361 QEIPQ 366
           QEIPQ
Sbjct: 361 QEIPQ 365

BLAST of Carg04039 vs. ExPASy TrEMBL
Match: A0A6J1KQC1 (uncharacterized protein LOC111496250 OS=Cucurbita maxima OX=3661 GN=LOC111496250 PE=4 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 7.3e-206
Identity = 363/365 (99.45%), Postives = 365/365 (100.00%), Query Frame = 0

Query: 1   MGAEPLLPTANESQPAAPLILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELEN 60
           MGAEPLLPTANES+PAAPLILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELEN
Sbjct: 1   MGAEPLLPTANESEPAAPLILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELEN 60

Query: 61  ILREVKSYILSSPDVLTPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCN 120
           ILREVKSYILSSPDVLTPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCN
Sbjct: 61  ILREVKSYILSSPDVLTPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCN 120

Query: 121 MSSNGVNLSRLRMIKGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWLV 180
           MSSNGVNLSRLRMIKGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWLV
Sbjct: 121 MSSNGVNLSRLRMIKGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWLV 180

Query: 181 MRYSIFNMEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANEDE 240
           MRYSIFNMEVIEAAVKMAKQ+KVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANEDE
Sbjct: 181 MRYSIFNMEVIEAAVKMAKQDKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANEDE 240

Query: 241 AKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATG 300
           AKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATG
Sbjct: 241 AKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATG 300

Query: 301 AGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPV 360
           AGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPV
Sbjct: 301 AGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPV 360

Query: 361 QEIPQ 366
           QEIPQ
Sbjct: 361 QEIPQ 365

BLAST of Carg04039 vs. ExPASy TrEMBL
Match: A0A0A0LPB8 (PfkB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G361810 PE=4 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 3.1e-188
Identity = 339/366 (92.62%), Postives = 351/366 (95.90%), Query Frame = 0

Query: 1   MGAEPLLPTANESQPAAPLILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELEN 60
           MGAEPLL T N+S+ A+PLILGLQPAALIDHV+RVD SLLDRIPGDRGGSMPVGMEELEN
Sbjct: 1   MGAEPLLHTVNDSE-ASPLILGLQPAALIDHVSRVDSSLLDRIPGDRGGSMPVGMEELEN 60

Query: 61  ILREVKSYILSSPDVL-TPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVC 120
           ILREVKSYILSSPD L TP KTIAGGSVANTIRGLSAGFG+SCGIIGA GDD+QGKLFV 
Sbjct: 61  ILREVKSYILSSPDDLTTPVKTIAGGSVANTIRGLSAGFGISCGIIGACGDDEQGKLFVS 120

Query: 121 NMSSNGVNLSRLRMIKGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWL 180
           NMSS+GVNLSRLRMIKGPTAQCVCLVDA+GNRTMRPSLSSAVKVQ NELTRDDFKG+KWL
Sbjct: 121 NMSSHGVNLSRLRMIKGPTAQCVCLVDALGNRTMRPSLSSAVKVQGNELTRDDFKGSKWL 180

Query: 181 VMRYSIFNMEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANED 240
           VMRYSIFN+EVIEAAVKMAKQEKVFVSLDLASFEMVRDFR PLLQLLESGDIDLCFANED
Sbjct: 181 VMRYSIFNIEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRLPLLQLLESGDIDLCFANED 240

Query: 241 EAKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDAT 300
           EAKELI GEKDADPEVALEFLAKHCQWA VTLGANGCIAKHGKEIVRVPAIGESKATDAT
Sbjct: 241 EAKELIRGEKDADPEVALEFLAKHCQWAAVTLGANGCIAKHGKEIVRVPAIGESKATDAT 300

Query: 301 GAGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLP 360
           GAGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIR+LGGEVTPENWQWMHKHLQIKGLP
Sbjct: 301 GAGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLP 360

Query: 361 VQEIPQ 366
           + EI Q
Sbjct: 361 LPEIRQ 365

BLAST of Carg04039 vs. ExPASy TrEMBL
Match: A0A6J1CTD9 (uncharacterized protein LOC111014039 OS=Momordica charantia OX=3673 GN=LOC111014039 PE=4 SV=1)

HSP 1 Score: 667.2 bits (1720), Expect = 4.0e-188
Identity = 334/366 (91.26%), Postives = 348/366 (95.08%), Query Frame = 0

Query: 1   MGAEPLLPT-ANESQPAAPLILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELE 60
           MGAEPLL    +     APL+LGLQPAAL+DH+ARVD SLLDRIPGDRGGS+PVGMEELE
Sbjct: 1   MGAEPLLQLHTHHDSETAPLVLGLQPAALVDHLARVDSSLLDRIPGDRGGSIPVGMEELE 60

Query: 61  NILREVKSYILSSPDVLTPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVC 120
           +ILR+VKSYILSSPD LTP KTIAGGSVANTIRGLSAGFGVSCGIIGA GDD+QGKLFVC
Sbjct: 61  HILRDVKSYILSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGACGDDEQGKLFVC 120

Query: 121 NMSSNGVNLSRLRMIKGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWL 180
           NMSSNGVNLSRLRM KG TAQCVCLVDA+GNRTMRPSLSSAVKVQ NELTRDDFKG+KWL
Sbjct: 121 NMSSNGVNLSRLRMTKGSTAQCVCLVDALGNRTMRPSLSSAVKVQGNELTRDDFKGSKWL 180

Query: 181 VMRYSIFNMEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANED 240
           VMRYSIFN+EVI+AAVKMAKQEKVFVSLDLASFEMVRDFR PLLQLLESGDIDLCFANED
Sbjct: 181 VMRYSIFNLEVIQAAVKMAKQEKVFVSLDLASFEMVRDFRLPLLQLLESGDIDLCFANED 240

Query: 241 EAKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDAT 300
           EAKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDAT
Sbjct: 241 EAKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDAT 300

Query: 301 GAGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLP 360
           GAGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIR+LGGEVTPENWQWMHKHLQIKGLP
Sbjct: 301 GAGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLP 360

Query: 361 VQEIPQ 366
           + EIPQ
Sbjct: 361 LPEIPQ 366

BLAST of Carg04039 vs. ExPASy TrEMBL
Match: A0A6J1HF87 (uncharacterized protein LOC111462499 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462499 PE=4 SV=1)

HSP 1 Score: 660.6 bits (1703), Expect = 3.8e-186
Identity = 332/365 (90.96%), Postives = 349/365 (95.62%), Query Frame = 0

Query: 1   MGAEPLLPTANESQPAAPLILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELEN 60
           MGAEPLL TAN+S+ AAPLILGLQPAALIDHVARVD SLLDRIPGDRGGSMPVGMEELEN
Sbjct: 1   MGAEPLLHTANDSE-AAPLILGLQPAALIDHVARVDSSLLDRIPGDRGGSMPVGMEELEN 60

Query: 61  ILREVKSYILSSPDVLTPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCN 120
           IL EVKSYI SSPD LTP KTIAGGSVANTIRGLSAGFGVSCGIIGAYGDD+QGKLFV N
Sbjct: 61  ILSEVKSYIRSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSN 120

Query: 121 MSSNGVNLSRLRMIKGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWLV 180
           M SNGVNLSRLRMIKGPTAQCVCLVD++GNRTMRP LSSAVKVQ NEL+RDDFKG+KWLV
Sbjct: 121 MGSNGVNLSRLRMIKGPTAQCVCLVDSLGNRTMRPCLSSAVKVQGNELSRDDFKGSKWLV 180

Query: 181 MRYSIFNMEVIEAAVKMAKQEKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANEDE 240
           MRYSIF++EVI+AAVKMAKQE+V VSLDLASFEMVR+FR PLL+LLESGDIDLCFANEDE
Sbjct: 181 MRYSIFSLEVIQAAVKMAKQERVLVSLDLASFEMVRNFRLPLLKLLESGDIDLCFANEDE 240

Query: 241 AKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATG 300
           AKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKE+VR+PAIGESKATDATG
Sbjct: 241 AKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDATG 300

Query: 301 AGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPV 360
           AGDLFASGFLYGLVKGLSL KCCQLGSCSGGSVIR+LGGEVTPEN QWMHKHLQIKGLP+
Sbjct: 301 AGDLFASGFLYGLVKGLSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLPL 360

Query: 361 QEIPQ 366
            EIPQ
Sbjct: 361 PEIPQ 364

BLAST of Carg04039 vs. TAIR 10
Match: AT1G19600.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 506.9 bits (1304), Expect = 1.3e-143
Identity = 247/349 (70.77%), Postives = 301/349 (86.25%), Query Frame = 0

Query: 15  PAAPLILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELENILREVKSYILSSPD 74
           P APL+LGLQPAALID+VA VD SLLD+IPGDRGGS+ V  +ELE++L+E+ ++I     
Sbjct: 10  PEAPLVLGLQPAALIDNVAPVDWSLLDQIPGDRGGSIAVQKDELEHMLKELDAHI----- 69

Query: 75  VLTPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDQQGKLFVCNMSSNGVNLSRLRMI 134
            + P K +AGGSV NT+RGLS GFGV+ GIIGAYGDD+QG+LFV NM  +GV++SRLR  
Sbjct: 70  SVAPLKKMAGGSVTNTVRGLSVGFGVATGIIGAYGDDEQGQLFVSNMGFSGVSISRLRKK 129

Query: 135 KGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWLVMRYSIFNMEVIEAA 194
           KG TAQCVCLVD  GNRTMRP LSSAVK+QA+EL+++DF G+KWLV+RY++ N++VI+AA
Sbjct: 130 KGSTAQCVCLVDDSGNRTMRPCLSSAVKIQADELSKEDFTGSKWLVLRYAVLNLQVIQAA 189

Query: 195 VKMAKQEKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCFANEDEAKELIGGEKDADPE 254
           ++ AKQE + VSLDLASFEMVR+ +S L QLLESG+IDLCFANEDEA EL+ GE++A PE
Sbjct: 190 IRFAKQEGLSVSLDLASFEMVRNSKSELRQLLESGNIDLCFANEDEAAELLRGEQEAGPE 249

Query: 255 VALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATGAGDLFASGFLYGLV 314
            ALEFL +HC+WAVVTLG+ GCIAKH KE+V + AIGE+ ATDATGAGDLFASGFLYGL+
Sbjct: 250 AALEFLGRHCRWAVVTLGSKGCIAKHDKEVVHISAIGETVATDATGAGDLFASGFLYGLI 309

Query: 315 KGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPVQEI 364
           KGLSLE+CC++GSCSGGSVIR+LGGEVTPENWQWMHK LQ+KGLPV +I
Sbjct: 310 KGLSLEECCKVGSCSGGSVIRALGGEVTPENWQWMHKQLQLKGLPVPDI 353

BLAST of Carg04039 vs. TAIR 10
Match: AT4G27600.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 70.5 bits (171), Expect = 3.2e-12
Identity = 73/323 (22.60%), Postives = 126/323 (39.01%), Query Frame = 0

Query: 10  ANESQPAAPL---ILGLQPAALIDHVARVDPSLLDRIPGDRGGSMPVGMEELENILREVK 69
           AN S  A+P    +LGL   A++D    VD   L ++  ++G    +  EE   +L+ + 
Sbjct: 110 ANNSSAASPERWDVLGL-GQAMVDFSGVVDDEFLKKLGLEKGTRKLINHEERGKLLQAMD 169

Query: 70  SYILSSPDVLTPFKTIAGGSVANTIRGLS-------AGFGVSCGIIGAYGDDQQGKLFVC 129
                       +K  AGGS++NT+  L+           ++  + G+ G D  G  +  
Sbjct: 170 G---------CSYKAAAGGSLSNTLVALARLGSQSICDRPLNVAMAGSIGGDPLGSFYGT 229

Query: 130 NMSSNGVNLSRLRMIKGPTAQCVCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWL 189
            +    VN     +  G T   + L      RTM     ++  V  +             
Sbjct: 230 KLRRANVNFLSAPIKDGTTGTVIVLTTPDAQRTMLAYQGTSSVVNYDSCLASLIAKTNVF 289

Query: 190 VMRYSIFNM----EVIEAAVKMAKQEKVFVSLDLASFEMVRDFRSPLLQLLESGDIDLCF 249
           V+   +F +      I  A + A +    V++  +    +         ++     D+ F
Sbjct: 290 VVEGYLFELPDTIRTITKACEEAHRNGALVAVTASDVSCIERHYDDFWDIV-GNYADIVF 349

Query: 250 ANEDEAKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKA 309
           AN DEA+       +  P  A  +++    +  VT G NG       E + +P       
Sbjct: 350 ANSDEARAFCHFSAEESPISATRYMSHFVPFVSVTDGINGSYIGVKGEAIYIPP-SPCVP 409

Query: 310 TDATGAGDLFASGFLYGLVKGLS 319
            D  GAGD +ASG LYG+++G+S
Sbjct: 410 VDTCGAGDAYASGILYGILRGVS 420

BLAST of Carg04039 vs. TAIR 10
Match: AT5G03300.1 (adenosine kinase 2 )

HSP 1 Score: 68.6 bits (166), Expect = 1.2e-11
Identity = 74/286 (25.87%), Postives = 126/286 (44.06%), Query Frame = 0

Query: 82  IAGGSVANTIRGLSAGFGV--SCGIIGAYGDDQQGKLFVCNMSSNGVNLSRLRMIKGPTA 141
           IAGG+  N+I+       +  +   +G+ G D+ G+    + ++ GVN+        PT 
Sbjct: 63  IAGGATQNSIKVAQWMLQIPGATSYMGSIGKDKYGEAMKKDATAAGVNVHYYEDESAPTG 122

Query: 142 QC-VCLVDAMGNRTMRPSLSSAVKVQANELTRDDFKGAKWLVMRYSI--FNMEVIEAAVK 201
            C VC+V   G R++  +LS+A   + + L + +          Y I  F + V   +++
Sbjct: 123 TCGVCVVG--GERSLIANLSAANCYKVDHLKKPENWALVEKAKFYYIAGFFLTVSPESIQ 182

Query: 202 M-----AKQEKVFVSLDLASF--EMVRDFRSPLLQLLESGDIDLCFANEDEAK---ELIG 261
           +     A   KVF     A F  E  +D +   L  +     D  F NE EA+    + G
Sbjct: 183 LVSEHAAANNKVFTMNLSAPFICEFFKDVQEKFLPYM-----DFVFGNETEARTFSRVHG 242

Query: 262 GEKDADPEVALEF------LAKHCQWAVVTLGANGCIAKHGKEIVRVPAI--GESKATDA 321
            E +   ++A++          + +  V+T GA+  +     ++ + P I   + K  D 
Sbjct: 243 WETEDVEQIAIKISQLPKATGTYKRTTVITQGADPVVVAEDGKVKKYPVIPLPKEKLVDT 302

Query: 322 TGAGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPE 345
            GAGD F  GF+  LVK  S+E+C + G C   +V+    G   PE
Sbjct: 303 NGAGDAFVGGFMSQLVKEKSIEECVKAG-CYASNVVIQRSGCTYPE 340

BLAST of Carg04039 vs. TAIR 10
Match: AT5G58730.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 52.0 bits (123), Expect = 1.2e-06
Identity = 29/77 (37.66%), Postives = 39/77 (50.65%), Query Frame = 0

Query: 262 KHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATGAGDLFASGFLYGLVKGLSLEK 321
           KH    VVT G  GC   H  + + VP    +K  D TGAGD F  G + GLV+GL++  
Sbjct: 210 KHLCCVVVTNGEKGCRIYHKDDEMTVPPF-LAKQVDPTGAGDSFLGGLIVGLVEGLTVPD 269

Query: 322 CCQLGSCSGGSVIRSLG 339
              LG+  G   +  +G
Sbjct: 270 AALLGNLFGSITVEHIG 285

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022951670.13.0e-206100.00uncharacterized protein LOC111454413 [Cucurbita moschata] >KAG6585583.1 hypothet... [more]
XP_023002394.11.5e-20599.45uncharacterized protein LOC111496250 [Cucurbita maxima][more]
XP_023538348.12.6e-20599.73uncharacterized protein LOC111799162 [Cucurbita pepo subsp. pepo][more]
XP_038886551.11.4e-19093.72uncharacterized sugar kinase slr0537 [Benincasa hispida][more]
XP_004152687.16.4e-18892.62uncharacterized protein LOC101214597 [Cucumis sativus] >KGN62617.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
Q554805.0e-1826.35Uncharacterized sugar kinase slr0537 OS=Synechocystis sp. (strain PCC 6803 / Kaz... [more]
Q5JDG95.5e-1729.30ADP-dependent ribose-1-phosphate kinase OS=Thermococcus kodakarensis (strain ATC... [more]
P774937.5e-1425.78Uncharacterized sugar kinase YdjH OS=Escherichia coli (strain K12) OX=83333 GN=y... [more]
D9TT101.4e-1224.91ATP-dependent 6-phosphofructokinase OS=Thermoanaerobacterium thermosaccharolytic... [more]
A4IPB31.6e-1124.075-dehydro-2-deoxygluconokinase OS=Geobacillus thermodenitrificans (strain NG80-2... [more]
Match NameE-valueIdentityDescription
A0A6J1GJH71.5e-206100.00uncharacterized protein LOC111454413 OS=Cucurbita moschata OX=3662 GN=LOC1114544... [more]
A0A6J1KQC17.3e-20699.45uncharacterized protein LOC111496250 OS=Cucurbita maxima OX=3661 GN=LOC111496250... [more]
A0A0A0LPB83.1e-18892.62PfkB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G361810 PE=4 S... [more]
A0A6J1CTD94.0e-18891.26uncharacterized protein LOC111014039 OS=Momordica charantia OX=3673 GN=LOC111014... [more]
A0A6J1HF873.8e-18690.96uncharacterized protein LOC111462499 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT1G19600.11.3e-14370.77pfkB-like carbohydrate kinase family protein [more]
AT4G27600.13.2e-1222.60pfkB-like carbohydrate kinase family protein [more]
AT5G03300.11.2e-1125.87adenosine kinase 2 [more]
AT5G58730.11.2e-0637.66pfkB-like carbohydrate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029056Ribokinase-likeGENE3D3.40.1190.20coord: 25..342
e-value: 1.7E-75
score: 256.1
IPR029056Ribokinase-likeSUPERFAMILY53613Ribokinase-likecoord: 27..339
IPR011611Carbohydrate kinase PfkBPFAMPF00294PfkBcoord: 79..339
e-value: 7.1E-40
score: 137.2
NoneNo IPR availablePANTHERPTHR43320:SF1CARBOHYDRATE KINASE-LIKEcoord: 12..356
NoneNo IPR availablePANTHERPTHR43320SUGAR KINASEcoord: 12..356
NoneNo IPR availableCDDcd01168adenosine_kinasecoord: 18..338
e-value: 3.39363E-95
score: 284.506

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg04039-RACarg04039-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
molecular_function GO:0016301 kinase activity