Carg04019 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04019
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Transcription initiation factor IIF subunit beta
Location	Carg_Chr12: 2621435 .. 2626965 (+)
RNA-Seq Expression	Carg04019
Synteny	Carg04019

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGATGAGAACGGCGGCGGTAGTAGCAGTAACTTGGATACCGGGAAGGCGGATAGATCAGTGTGGCTGATGAAGTGCCCGTTGTTGGTCGCCAAGTCATGGCAGTCTCATCCTCCATCTGATCCTCTTCCTCTCGCTAAGGTCATCCTCTCTCTCGATCCTCTTCAATCCGACGAGTCCTCTTCTCTCCAGGTATTTTTAACAATTGATGTTTACGCGGCGAACTAATTAAACTCTTTCTCGAGTTTTCTCAGTAGGTTTAATTGTTGTTGGCTTTGAATCTCGTGAATTATATTGTTTCTGATTGTGAATTTGATCGAGACTTTGATTGTGGAAGGATAACCCTAGATTTTCTCTCGTTTGAACTTTGATCTCTTGGGAGGGGTAGCTTGAATCTATGATACTTGTTGCAAAGTTCTTTATTAGTTCATTTATCGTAATTACTGAGCATTGGCCTCAAATTGTGCAAGATTTAAGTCTTTTTTTGAACTATTTGAATCATGAATCTTAAATCTTCATGAAAGGTTGCGTACTTGGATAACGTCTCCATTGGTTTGTGTTGTGAAACCTAGTTTTTTGGCTGTGGTTAAGGCCCTCACTTCCCTTGATGCGTTTGCTTGAAATCACATAAGGTTTAAGTGTCCTAAAAGAGTTAACATCTTCACTTGGGAGTTCATTCACGGTGGTATTAACACTCGAGACAAATTAGAGCTAGGAACACTCTTTTCACTCTATGTTCGAGTTGGTACATGATGTGCAATGAAAGTGAAGGGACGAAGTACAGTTCCCCTTCGTCTTAGTTAACAAACATTTTGTCGGCGTTTCTCTCACTCGAAGAGATCACAAAAAGGCTTCTCAAATGGTGTTAATGACAAACAAATTATTATTACTAAGGAGAGTGGAAAGGGTACACCATATTGAGGTGTTAAACTGAGCTTCCTCCCATATGTTCTTCCAGTTCTTCTTCCTCCATCAAAATTATTGGAAGTCGTTTCTCTCCATATTGACCAGAGAGTGCTCATAACGCGTCCATATTGGATCCAAATACATTTCTCCGTTGGATGGCTCTTTCCCACACGTGAGTCACACATGTGAAACCCAATTAAATTGTAACTTTACAACAACTAACAAAAGAAGCTGATTGCAATACTCATCAAGTACAAAAAACTGATTGATCAAATTAAAAGTTCATGGGTATGATTGCGACATTTAACAAAGTTAAGGGGGCTAATTGATCAAATTGAAAGTTCAAGGGTGTGATTGTAACAAACTCCATAGTTCTGGGTGGTTCTGCAGTTTTTCCTTTTTGTATTGTTTGGCTCTACTCAATATTTTGTCGAATTATATTAATGAAATTTTGATTTTTCATCACAAAAAACAATCATTATGTGTTCTGTTATTTCTATGTCGCTGTTCTGTATTTTCTGCTTTTTTTTTTCTTTTGCTTTCGTTAATTTTTTCTTTCCGTTGTTTGTGCCTGCTAGTTCTGTACTCTCTTAGTCTTTGTTGGACTATACGCTCTCAGATATACACATATGATGCATATATATAGAGGAAGCAAATTATTTATTTTACATAGCCAGCAATGATGGTGACTGTAGCCTGTAGCTCATTATTTATTGTTTTTTTTTTTCCGACACTGTTTTCCCTTATATGCTTCAAAATGTACCCCAGTTCAAGATGGAGATGGCTGGAACTGAGACTGGAAACGTGCCAAAAAGTTTTTCCTTAAACATGTTTAAAGACTTTGTCCCCATGTGTGTCTTTTCTGAGGCAAGTCAAGGTGAGCATCATGATCCACATCTGAAATCCATTATTTATTTTTCGGGCGTTACTGTTATCCTGAATGTGGCTTTATAAGTATATCCCTGGTTTCGAGAAGTGTTTCTTCCTGTTTTGAACATCAATTTTGACTGAGATCTGTTAAAAAATAGTAATAAAACATCAATTTCGACTGATGGTGATGTAGATTTTTATTTCATTTTTATTAAATTTGTATTTTCTTAAATAAAGAACAGGGAAATTCATTGAAACAAAAAGGGGTAGAGCATAGAGGCCAGAAGGAAGAGGTATCCCTTTGCCAAGGAGGGGTATAGACAGGCCTCTCCTTAAACTAGCAAAAAATGGTTACAAAAATTCCCTCGAAGAGACACTCCAAATGGAGAGGCCACTGTGAGGTTAAATTTTGTCCTAAAAGTATCCCCAAATGTCATATTGGCCCTTTTTGTCTAGATAGACAGTAAAAGTGCTATACCTGTGGTTTTCAATAAGATTAGCTTTTAAGTCAATTCTATGAGAGCAGTTCTGGGAGCCAATCAGCAATATTGTTAGGGAACCAGAAATTGAGCCCAAAATTTTTCGGAAGGGATGCAACCTGTTAGTCACAAACTCGTAATGAATGAAAAACTGGTCTTTACCCTCATATTCTTTCTTATTTTTAGGAGTGAGTGGAGATAGTGGAGGAGATATTTTCTTGAGTTTTTTAACTCTTTAATGCCATGGATGATGGACCTGATATGGAACTTTTTTTCTTTTTGGAACTTGCCACCACAAATGCACGTTTTCAGTTGGATACTCAACTTAGACTTCATGCAATTGGATTCAAGTTGTTAAGTAGTTAAGTGCTCAATTTACAACAAATGAGCTGTTTCTTTAAGTCAGAGCCTGATCGTACTTAATTGGTTCGAGTGGTTCCTCTTCTCAATTTCTCTGTGAAAAAGGACCCCTTCAAATTAACATCCCTGCCTTGAGAATTCTCTCTTCAAATGTCTTTGATAAGAAATCTTTAGTATTTGGCATAGTATAAATCTTGGGGAGGAGGTTCTCTGTATACTAGAATTGGCTCTAATATCTTCCAGAAAACGAATTTTGTTCGATTCCAAACTTTGATAAATATGCATCTTTTCTCATAAAAGATGAAGCCTCAAGGCATAACGTCTATGGTTACCTCCAATTTTCCGTATGAATCACCCAAGAGAGTTGGAACCAAGTTTGCTCCAATGAACTCTCTTCTATAGGGCATTCTCCTCGTTTCCATGTGAATTTTTTAGTCTTTCTTTGTCTCTAAGATTCCCAACCCCTATTCTTCCACAAGAAGTTGAGACTTGAGAGTCGTTGATTCCACTCTTCTTCCAAGTGAAGTCTCCCTTAGTGTTTTCTCCATAATTCTAGTCTTTGAAAGGCTTCTAAATAGGGAGCAATAATATTTTGGAATTGGATGGTTAGTTCGTCAATCCAAGTTATTATTGTTGTTTTTTGTGGTAAATTTTATGTTCCTTTCAACACGGTCTAAATTCTCTCTGTTCCAATTTCAGAAAGCTTTTTGTAATCTTCCTGGATTGGGGCCCGGGCTCGTGTTTCCTTCCCTTTTGTAATTTGTCCTCTCTAAGGACTTCCCTTCTCAACCAACTTCGTAGCCTTCATTTTATACAAGGAGTTAGCTGAATTTGTTGGTGCATTTGCTGATGACCCACTTTCCGAAAAGAGATCTTGGATCTCTCTGCAATCTTCTTTGAAAATGTTGGGTTTTCATTCCTTTTCAACACTTCGGCACTTATACCGCATTGGCCTGATCTAGGGGGCTTTCAATTTACAAGTCTTTAGCTGCTTCATGGATACCCTCCTCCCCAATAGGTCCCTCTAGCCACTCACTTCAAGTTGAGAAAATGGCTCTGCAACAAACATTATGGAAGAGAGACCTCTTATTGTTGTTTGTTGAGGATAGCTTGCCCAAATAATCTATTGCGTTGTTCACTAACTCTTCTTCAACACTGGTTTTTTACCTTTAACATCAATTGACAAAAGCATGTACCCTCTGACCAAGCGGTCAAGGGTTCAAATCTTCCACATATTTAAAAGTTATGGATAATGGTTTCTCCAACGAATCTTTCATAGAAATCAGCTTGAAAACTATTTGCTTTAAGTTAGAATGAAGATGCATGCACAGAAATCAGCTCATGAATTATCATTTTGATTGTTCGTTATTGAATTTGACTCTTTAAAGTTACTCCAAAAAAAATTCTTTTGTAGGTAAGATGTCAATGGAAGGGAAGGTAGAACATAAATTCGATATGAAGCCTCATAGTGAAAATCTTGAAATGTATGGGAAATTGTGCCGTGAAAGGACAAACAAGTCCATGGTCAAGAATAGACAAATTCAAGTACATCATCAAAACCCCTTATTTTCCATACTTCCCTTTTAGCATTCCATGTTTGCAATCAACTTTACATTTTACCGTTCGCTTTATGTGGTGGTAAGGTGATAGACAATGACCGTGGGGTGCATATGAGGCCAATGCCTGGTATGGTCGGTTTGATCAGTTCAACTTCAAAGGTACATCTTTTCTCTTCTTTTACTTTCATCGTTTTTCTGAACTTTCGTGTTCGAAGTATGATTATATGATTAATCATTTATGGCAGGATAAGAGGAAGGTGGCGCCAGTTAAACAACCAGAAGTAAAAAGAACTAGAAGGGATCGTGGGGAACTGGAAGATATAATGTTCAAGCTATTTGAAAGACAGCCGAATTGGGCCCTAAAGCAGCTAGTCCAAGAGACTGATCAACCTGCAGTACGTCTATTCATCATTTCCTCATTTATCATTGTTTTTAAAAAAATATTATGCTTTTTCGGACGATATTGACTGAGAAGATCGGCATATTACCCTCTTGACATACACGAGACACCGTACAACCTTCCCTTCCCCAAAACTCAAAACTTCATTATAGATCAAAAAACATAAGAGATTATAAATTTTGTGGTTATATAAAATGTTCATCATATTTGTTCTTGGCTTTTGTGTTATATAAAATGATGTCAGAGTGCTCAAAACAATTGGTGTGCCTTCTCTATGCAGCAATTCTTGAAAGAGATACTGAATGAGCTTTGTGTCTACAACAAAAGAGGGACTAATCAAGGAACCTACGAGCTTAAGCCCGAATACAAGAAATCGGTCGAGGATACCGGCGGAGACTAGGTTCGTTTTCGACTGTATCATTTTATACATTCCCTTCTCTTTGGGGTAGGTGGAAACGATCTGGAATAAATATGCCAATTAAGAAGAAAAAAAGAAAGGATTTTGCTTTTGTTATAATTTCATGGGAGGGAAAGATGGGAGGATGAAATGAAATAATTGATAGGCAGCTTTATACTGAAGTGAAACCAAAGGTGAAATGAAAGTTGGGGGGAAGTTCTTATGTCATGTTTATGCATATTACGTACATGGTCGGAAGTTGCTAAATGTATCTGTATAATGACTTTTATGAGCATGCCAAGATCTTGATTGATTGTTCATTATCTTAAAATTTGCTGTAGTTTCTTCAAGAATTTGTTGTTTGATTGGCTGTTTGATTTGAAAATATATCTTATACTTGTTCATATGAGATATTTAGGCTGTGTTTGGTTGGTTATTTGATTCTTTTCTTTGAAAATTAGGAATTTAGAGTCCGTTTGATAATGTTTTCGTTGCCCATTTCTCGTTTCTTTGTTTCGTTAAGAAAAATAGGAGTTTTCTTGTTATTTACTTTTAAT

mRNA sequence

ATGGAAGATGAGAACGGCGGCGGTAGTAGCAGTAACTTGGATACCGGGAAGGCGGATAGATCAGTGTGGCTGATGAAGTGCCCGTTGTTGGTCGCCAAGTCATGGCAGTCTCATCCTCCATCTGATCCTCTTCCTCTCGCTAAGGTCATCCTCTCTCTCGATCCTCTTCAATCCGACGAGTCCTCTTCTCTCCAGTTCAAGATGGAGATGGCTGGAACTGAGACTGGAAACGTGCCAAAAAGTTTTTCCTTAAACATGTTTAAAGACTTTGTCCCCATGTGTGTCTTTTCTGAGGCAAGTCAAGGTAAGATGTCAATGGAAGGGAAGGTAGAACATAAATTCGATATGAAGCCTCATAGTGAAAATCTTGAAATGTATGGGAAATTGTGCCGTGAAAGGACAAACAAGTCCATGGTCAAGAATAGACAAATTCAAGTGATAGACAATGACCGTGGGGTGCATATGAGGCCAATGCCTGGTATGGTCGGTTTGATCAGTTCAACTTCAAAGGATAAGAGGAAGGTGGCGCCAGTTAAACAACCAGAAGTAAAAAGAACTAGAAGGGATCGTGGGGAACTGGAAGATATAATGTTCAAGCTATTTGAAAGACAGCCGAATTGGGCCCTAAAGCAGCTAGTCCAAGAGACTGATCAACCTGCACAATTCTTGAAAGAGATACTGAATGAGCTTTGTGTCTACAACAAAAGAGGGACTAATCAAGGAACCTACGAGCTTAAGCCCGAATACAAGAAATCGGTCGAGGATACCGGCGGAGACTAGGTTCGTTTTCGACTGTATCATTTTATACATTCCCTTCTCTTTGGGGTAGGTGGAAACGATCTGGAATAAATATGCCAATTAAGAAGAAAAAAAGAAAGGATTTTGCTTTTGTTATAATTTCATGGGAGGGAAAGATGGGAGGATGAAATGAAATAATTGATAGGCAGCTTTATACTGAAGTGAAACCAAAGGTGAAATGAAAGTTGGGGGGAAGTTCTTATGTCATGTTTATGCATATTACGTACATGGTCGGAAGTTGCTAAATGTATCTGTATAATGACTTTTATGAGCATGCCAAGATCTTGATTGATTGTTCATTATCTTAAAATTTGCTGTAGTTTCTTCAAGAATTTGTTGTTTGATTGGCTGTTTGATTTGAAAATATATCTTATACTTGTTCATATGAGATATTTAGGCTGTGTTTGGTTGGTTATTTGATTCTTTTCTTTGAAAATTAGGAATTTAGAGTCCGTTTGATAATGTTTTCGTTGCCCATTTCTCGTTTCTTTGTTTCGTTAAGAAAAATAGGAGTTTTCTTGTTATTTACTTTTAAT

Coding sequence (CDS)

ATGGAAGATGAGAACGGCGGCGGTAGTAGCAGTAACTTGGATACCGGGAAGGCGGATAGATCAGTGTGGCTGATGAAGTGCCCGTTGTTGGTCGCCAAGTCATGGCAGTCTCATCCTCCATCTGATCCTCTTCCTCTCGCTAAGGTCATCCTCTCTCTCGATCCTCTTCAATCCGACGAGTCCTCTTCTCTCCAGTTCAAGATGGAGATGGCTGGAACTGAGACTGGAAACGTGCCAAAAAGTTTTTCCTTAAACATGTTTAAAGACTTTGTCCCCATGTGTGTCTTTTCTGAGGCAAGTCAAGGTAAGATGTCAATGGAAGGGAAGGTAGAACATAAATTCGATATGAAGCCTCATAGTGAAAATCTTGAAATGTATGGGAAATTGTGCCGTGAAAGGACAAACAAGTCCATGGTCAAGAATAGACAAATTCAAGTGATAGACAATGACCGTGGGGTGCATATGAGGCCAATGCCTGGTATGGTCGGTTTGATCAGTTCAACTTCAAAGGATAAGAGGAAGGTGGCGCCAGTTAAACAACCAGAAGTAAAAAGAACTAGAAGGGATCGTGGGGAACTGGAAGATATAATGTTCAAGCTATTTGAAAGACAGCCGAATTGGGCCCTAAAGCAGCTAGTCCAAGAGACTGATCAACCTGCACAATTCTTGAAAGAGATACTGAATGAGCTTTGTGTCTACAACAAAAGAGGGACTAATCAAGGAACCTACGAGCTTAAGCCCGAATACAAGAAATCGGTCGAGGATACCGGCGGAGACTAG

Protein sequence

MEDENGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPHSENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVKQPEVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQGTYELKPEYKKSVEDTGGD

Homology

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BLAST of Carg04019 vs. NCBI nr
Match: XP_022951326.1 (transcription initiation factor IIF subunit beta [Cucurbita moschata] >XP_023537305.1 transcription initiation factor IIF subunit beta [Cucurbita pepo subsp. pepo] >KAG6585564.1 hypothetical protein SDJN03_18297, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020478.1 tfg2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 521.2 bits (1341), Expect = 5.2e-144
Identity = 259/259 (100.00%), Postives = 259/259 (100.00%), Query Frame = 0


Query: 1   MEDENGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDE 60
           MEDENGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDE
Sbjct: 1   MEDENGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDE 60

Query: 61  SSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPHS 120
           SSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPHS
Sbjct: 61  SSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPHS 120

Query: 121 ENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVKQ 180
           ENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVKQ
Sbjct: 121 ENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVKQ 180

Query: 181 PEVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQ 240
           PEVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQ
Sbjct: 181 PEVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQ 240

Query: 241 GTYELKPEYKKSVEDTGGD 260
           GTYELKPEYKKSVEDTGGD
Sbjct: 241 GTYELKPEYKKSVEDTGGD 259

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BLAST of Carg04019 vs. NCBI nr
Match: XP_023002475.1 (transcription initiation factor IIF subunit beta [Cucurbita maxima])

HSP 1 Score: 519.6 bits (1337), Expect = 1.5e-143
Identity = 258/259 (99.61%), Postives = 259/259 (100.00%), Query Frame = 0


Query: 1   MEDENGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDE 60
           MEDENGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDE
Sbjct: 1   MEDENGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDE 60

Query: 61  SSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPHS 120
           SSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPHS
Sbjct: 61  SSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPHS 120

Query: 121 ENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVKQ 180
           ENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVKQ
Sbjct: 121 ENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVKQ 180

Query: 181 PEVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQ 240
           PEVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQ
Sbjct: 181 PEVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQ 240

Query: 241 GTYELKPEYKKSVEDTGGD 260
           GTYELKPEYKKSVEDTGG+
Sbjct: 241 GTYELKPEYKKSVEDTGGE 259

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BLAST of Carg04019 vs. NCBI nr
Match: XP_038884084.1 (transcription initiation factor IIF subunit beta-like [Benincasa hispida])

HSP 1 Score: 511.5 bits (1316), Expect = 4.2e-141
Identity = 253/259 (97.68%), Postives = 258/259 (99.61%), Query Frame = 0


Query: 1   MEDENGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDE 60
           MEDENGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQ+HPPSDPLPLAKVILSLDPLQSDE
Sbjct: 1   MEDENGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQAHPPSDPLPLAKVILSLDPLQSDE 60

Query: 61  SSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPHS 120
           SSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPHS
Sbjct: 61  SSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPHS 120

Query: 121 ENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVKQ 180
           ENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSK+K+KVAPVKQ
Sbjct: 121 ENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKEKKKVAPVKQ 180

Query: 181 PEVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQ 240
            +VKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQ
Sbjct: 181 SDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQ 240

Query: 241 GTYELKPEYKKSVEDTGGD 260
           GTYELKPEYKKSVEDTGG+
Sbjct: 241 GTYELKPEYKKSVEDTGGE 259

[Close]

BLAST of Carg04019 vs. NCBI nr
Match: XP_008444765.1 (PREDICTED: transcription initiation factor IIF subunit beta-like [Cucumis melo] >KAA0065193.1 transcription initiation factor IIF subunit beta-like [Cucumis melo var. makuwa])

HSP 1 Score: 502.3 bits (1292), Expect = 2.5e-138
Identity = 250/260 (96.15%), Postives = 258/260 (99.23%), Query Frame = 0


Query: 1   MEDENG-GGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSD 60
           MED++G GGSSSNLDTGKADRSVWLMKCPLLVAKSWQ+HPPSDPLPLAKVILSLDPLQSD
Sbjct: 1   MEDDHGSGGSSSNLDTGKADRSVWLMKCPLLVAKSWQAHPPSDPLPLAKVILSLDPLQSD 60

Query: 61  ESSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPH 120
           ESSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGK+SMEGKVEHKFDMKPH
Sbjct: 61  ESSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPH 120

Query: 121 SENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVK 180
           SENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSK+K+KVAPVK
Sbjct: 121 SENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKEKKKVAPVK 180

Query: 181 QPEVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTN 240
           Q +VKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTN
Sbjct: 181 QSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTN 240

Query: 241 QGTYELKPEYKKSVEDTGGD 260
           QGTYELKPEYKKSVEDTGG+
Sbjct: 241 QGTYELKPEYKKSVEDTGGE 260

[Close]

BLAST of Carg04019 vs. NCBI nr
Match: XP_004152667.1 (transcription initiation factor IIF subunit beta [Cucumis sativus] >KGN62646.1 hypothetical protein Csa_021785 [Cucumis sativus])

HSP 1 Score: 500.4 bits (1287), Expect = 9.6e-138
Identity = 250/260 (96.15%), Postives = 257/260 (98.85%), Query Frame = 0


Query: 1   MEDENG-GGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSD 60
           MEDE+G GGSSSNLDTGKADRSVWLMKCPLLVAKSWQ+HPPSD LPLAKVILSLDPLQSD
Sbjct: 1   MEDEHGSGGSSSNLDTGKADRSVWLMKCPLLVAKSWQAHPPSDSLPLAKVILSLDPLQSD 60

Query: 61  ESSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPH 120
           ESSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGK+SMEGKVEHKFDMKPH
Sbjct: 61  ESSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPH 120

Query: 121 SENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVK 180
           SENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSK+K+KVAPVK
Sbjct: 121 SENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKEKKKVAPVK 180

Query: 181 QPEVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTN 240
           Q +VKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTN
Sbjct: 181 QSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTN 240

Query: 241 QGTYELKPEYKKSVEDTGGD 260
           QGTYELKPEYKKSVEDTGG+
Sbjct: 241 QGTYELKPEYKKSVEDTGGE 260

[Close]

BLAST of Carg04019 vs. ExPASy Swiss-Prot
Match: O94424 (Transcription initiation factor IIF subunit beta OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tfg2 PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 4.0e-22
Identity = 83/259 (32.05%), Postives = 125/259 (48.26%), Query Frame = 0


Query: 2   EDENGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDES 61
           ED+     + +LD G+    VWL+K P  +   W S P  D   L  V +  D +Q    
Sbjct: 12  EDDRYEDDAGDLDLGQIGSRVWLVKIPKFLMDKWNSIPEDDAANLGCVRVKNDEIQLLLQ 71

Query: 62  SSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSME-----GKVEHKFDM 121
           +S          E  +VPK ++L +   FV        S+   SM+     G V H+ ++
Sbjct: 72  NS---------PENADVPKIYNLRVMNKFVRNSYVFRESETSSSMKSTALVGTVAHECNV 131

Query: 122 KPHSENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLIS-STSKDKRKV 181
            P     + Y ++ ++R   +    R++Q+ID DRG  +   PG +G  S ST+   R V
Sbjct: 132 SPVIN--DDYRRVMQKRALAASAPKRKVQMID-DRGGSLL-APGTLGSRSRSTTSFIRNV 191

Query: 182 APVKQPEVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNK 241
            P     +K +R  R EL DI+FK FE    W LK L +   QP  +LKE+L+ + + NK
Sbjct: 192 KPRTGEGLKNSRIPRNELLDILFKCFEDYEYWTLKGLREYVKQPEVYLKEVLDSIAILNK 251

Query: 242 RGTNQGTYELKPEYKKSVE 255
           RG     Y LKPEYK +++
Sbjct: 252 RGPYALKYSLKPEYKGTMD 257

[Close]

BLAST of Carg04019 vs. ExPASy Swiss-Prot
Match: Q54KT7 (General transcription factor IIF subunit 2 OS=Dictyostelium discoideum OX=44689 GN=gtf2f2 PE=3 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 1.1e-16
Identity = 70/257 (27.24%), Postives = 113/257 (43.97%), Query Frame = 0


Query: 1   MEDENGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDE 60
           M +E        ++T  AD  VWL+K P  +++SWQ         + +  +    ++  +
Sbjct: 1   MSEEKNDALVGEVNTDNADNQVWLIKVPKFLSESWQK--------IGQGEIGQIHIKGGD 60

Query: 61  SSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPHS 120
           + SL +     G + G   +  +     D  P+ +FSE   G +++EG +  + D+K   
Sbjct: 61  NISLSY-----GPDVGQEFQLITTANTLDNQPLKIFSEDKDGALALEGNIGLRCDIKIDV 120

Query: 121 ENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKV----- 180
           E+   Y  L + R  K   K R  QVID               L + T  D  KV     
Sbjct: 121 ES-PQYRDLMKSRHTKYNTKTRMTQVIDESE------------LFTPTLFDANKVKVSTV 180

Query: 181 --APVKQPEVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVY 240
                K+   K+ +    E+ D++F  F  + +  LK L   T+QP   LK IL ++C+ 
Sbjct: 181 GITQKKKSTDKKEKLPEDEVLDLIFSAFRAEKHLDLKTLESFTEQPKNHLKTILEKVCIL 231

Query: 241 NKRGTNQGTYELKPEYK 251
           NKRG     YELKPE++
Sbjct: 241 NKRGPYHHLYELKPEFR 231

[Close]

BLAST of Carg04019 vs. ExPASy Swiss-Prot
Match: P41900 (General transcription factor IIF subunit 2 OS=Drosophila melanogaster OX=7227 GN=TfIIFbeta PE=2 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 1.1e-16
Identity = 72/260 (27.69%), Postives = 122/260 (46.92%), Query Frame = 0


Query: 12  NLDTGKADRSVWLMKCPLLVAKSWQSHPPS----------DPLPLAKVILSLDP----LQ 71
           +LD   A R VWL+K P  +A+ W+  P +           P   A+V LSL P    L 
Sbjct: 15  DLDLSNAGRGVWLVKVPKYIAQKWEKAPTNMDVGKLRINKTPGQKAQVSLSLTPAVLALD 74

Query: 72  SDESSSLQFKMEMAGTETGNVPKSFSLNMFKDFVP-------MCVFSEASQGKMSMEGKV 131
            +E    +  ++++      V K  +L +F    P           ++    K+ MEG++
Sbjct: 75  PEEKIPTEHILDVS-----QVTKQ-TLGVFSHMAPSDGKENSTTSAAQPDNEKLYMEGRI 134

Query: 132 EHKFDMKPHSENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSK 191
             K + +P ++N   Y KL  E   K+    R++Q ID     + +P+      I    +
Sbjct: 135 VQKLECRPIADN--CYMKLKLESIRKASEPQRRVQPIDK-IVQNFKPVKDHAHNIEYRER 194

Query: 192 DKRKVAPVKQPEVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNEL 251
                   K+ E K+ R D+  + D++F  FE+   + +K LV+ T+QP  +LKEIL ++
Sbjct: 195 --------KKAEGKKARDDKNAVMDMLFHAFEKHQYYNIKDLVKITNQPISYLKEILKDV 254

[Close]

BLAST of Carg04019 vs. ExPASy Swiss-Prot
Match: Q2T9L9 (General transcription factor IIF subunit 2 OS=Bos taurus OX=9913 GN=GTF2F2 PE=2 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.9e-16
Identity = 70/247 (28.34%), Postives = 115/247 (46.56%), Query Frame = 0


Query: 12  NLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQFKMEMA 71
           +L   K +  VWL+K P  +++ W   P    +   ++  +    Q     S     ++A
Sbjct: 8   DLTGAKQNTGVWLVKVPKYLSQQWAKAPGRGEVGKLRIAKN----QGRTEVSFTLNEDLA 67

Query: 72  GT-ETGNVPKSFSLNMFKDFV-------PMCVFSEASQGKMSMEGKVEHKFDMKPHSENL 131
              + G  P S S      FV        + VF+E+S  K+S+EG V  + + +P +   
Sbjct: 68  NIHDIGGKPASVSAPREHPFVLQSVGGQTLTVFTESSSDKLSLEGIVVQRAECRPAAN-- 127

Query: 132 EMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVKQPEV 191
           E Y +L R +  +S    R  Q +D     + +P+      I    K        K+ + 
Sbjct: 128 ENYMRLKRLQIEESSKPVRLSQQLDKVVTTNYKPVANHQYNIEYERK--------KKEDG 187

Query: 192 KRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQGTY 251
           KR R D+  + D++F  FE+   + LK LV  T QP  +LK+IL E+ V N +G ++ T+
Sbjct: 188 KRARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVSYLKDILKEIGVQNVKGIHKNTW 240

[Close]

BLAST of Carg04019 vs. ExPASy Swiss-Prot
Match: Q01750 (General transcription factor IIF subunit 2 OS=Rattus norvegicus OX=10116 GN=Gtf2f2 PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.9e-16
Identity = 71/247 (28.74%), Postives = 114/247 (46.15%), Query Frame = 0


Query: 12  NLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDESSSLQFKMEMA 71
           +L   K +  VWL+K P  +++ W   P    +   ++  +    Q     S     ++A
Sbjct: 8   DLTGAKQNTGVWLVKVPKYLSQQWAKAPGRGEVGKLRIAKN----QGRTEVSFTLNEDLA 67

Query: 72  GT-ETGNVPKSFSLNMFKDFV-------PMCVFSEASQGKMSMEGKVEHKFDMKPHSENL 131
              + G  P S S      FV        + VF+E+S  K+S+EG V  + + +P +   
Sbjct: 68  NIHDIGGKPASVSAPREHPFVLQSVGGQTLTVFTESSSDKLSLEGIVVQRAECRPAAS-- 127

Query: 132 EMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVKQPEV 191
           E Y KL R +  +S    R  Q  D     + +P+      I    K        K+ + 
Sbjct: 128 ENYMKLKRLQIEESSKPVRLSQQADKVVTTNYKPVANHQYNIEYERK--------KKEDG 187

Query: 192 KRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQGTY 251
           KR R D+  + D++F  FE+   + LK LV  T QP  +LKEIL E+ + N +G ++ T+
Sbjct: 188 KRARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVGYLKEILKEIGIQNVKGIHKNTW 240

[Close]

BLAST of Carg04019 vs. ExPASy TrEMBL
Match: A0A6J1GH97 (Transcription initiation factor IIF subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111454191 PE=3 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 2.5e-144
Identity = 259/259 (100.00%), Postives = 259/259 (100.00%), Query Frame = 0


Query: 1   MEDENGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDE 60
           MEDENGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDE
Sbjct: 1   MEDENGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDE 60

Query: 61  SSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPHS 120
           SSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPHS
Sbjct: 61  SSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPHS 120

Query: 121 ENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVKQ 180
           ENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVKQ
Sbjct: 121 ENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVKQ 180

Query: 181 PEVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQ 240
           PEVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQ
Sbjct: 181 PEVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQ 240

Query: 241 GTYELKPEYKKSVEDTGGD 260
           GTYELKPEYKKSVEDTGGD
Sbjct: 241 GTYELKPEYKKSVEDTGGD 259

[Close]

BLAST of Carg04019 vs. ExPASy TrEMBL
Match: A0A6J1KP21 (Transcription initiation factor IIF subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111496301 PE=3 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 7.4e-144
Identity = 258/259 (99.61%), Postives = 259/259 (100.00%), Query Frame = 0


Query: 1   MEDENGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDE 60
           MEDENGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDE
Sbjct: 1   MEDENGGGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSDE 60

Query: 61  SSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPHS 120
           SSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPHS
Sbjct: 61  SSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPHS 120

Query: 121 ENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVKQ 180
           ENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVKQ
Sbjct: 121 ENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVKQ 180

Query: 181 PEVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQ 240
           PEVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQ
Sbjct: 181 PEVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQ 240

Query: 241 GTYELKPEYKKSVEDTGGD 260
           GTYELKPEYKKSVEDTGG+
Sbjct: 241 GTYELKPEYKKSVEDTGGE 259

[Close]

BLAST of Carg04019 vs. ExPASy TrEMBL
Match: A0A5A7VAJ9 (Transcription initiation factor IIF subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005300 PE=3 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.2e-138
Identity = 250/260 (96.15%), Postives = 258/260 (99.23%), Query Frame = 0


Query: 1   MEDENG-GGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSD 60
           MED++G GGSSSNLDTGKADRSVWLMKCPLLVAKSWQ+HPPSDPLPLAKVILSLDPLQSD
Sbjct: 1   MEDDHGSGGSSSNLDTGKADRSVWLMKCPLLVAKSWQAHPPSDPLPLAKVILSLDPLQSD 60

Query: 61  ESSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPH 120
           ESSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGK+SMEGKVEHKFDMKPH
Sbjct: 61  ESSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPH 120

Query: 121 SENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVK 180
           SENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSK+K+KVAPVK
Sbjct: 121 SENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKEKKKVAPVK 180

Query: 181 QPEVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTN 240
           Q +VKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTN
Sbjct: 181 QSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTN 240

Query: 241 QGTYELKPEYKKSVEDTGGD 260
           QGTYELKPEYKKSVEDTGG+
Sbjct: 241 QGTYELKPEYKKSVEDTGGE 260

[Close]

BLAST of Carg04019 vs. ExPASy TrEMBL
Match: A0A1S3BBV5 (Transcription initiation factor IIF subunit beta OS=Cucumis melo OX=3656 GN=LOC103488014 PE=3 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.2e-138
Identity = 250/260 (96.15%), Postives = 258/260 (99.23%), Query Frame = 0


Query: 1   MEDENG-GGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSD 60
           MED++G GGSSSNLDTGKADRSVWLMKCPLLVAKSWQ+HPPSDPLPLAKVILSLDPLQSD
Sbjct: 1   MEDDHGSGGSSSNLDTGKADRSVWLMKCPLLVAKSWQAHPPSDPLPLAKVILSLDPLQSD 60

Query: 61  ESSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPH 120
           ESSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGK+SMEGKVEHKFDMKPH
Sbjct: 61  ESSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPH 120

Query: 121 SENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVK 180
           SENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSK+K+KVAPVK
Sbjct: 121 SENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKEKKKVAPVK 180

Query: 181 QPEVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTN 240
           Q +VKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTN
Sbjct: 181 QSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTN 240

Query: 241 QGTYELKPEYKKSVEDTGGD 260
           QGTYELKPEYKKSVEDTGG+
Sbjct: 241 QGTYELKPEYKKSVEDTGGE 260

[Close]

BLAST of Carg04019 vs. ExPASy TrEMBL
Match: A0A0A0LRL0 (Transcription initiation factor IIF subunit beta OS=Cucumis sativus OX=3659 GN=Csa_2G363560 PE=3 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 4.6e-138
Identity = 250/260 (96.15%), Postives = 257/260 (98.85%), Query Frame = 0


Query: 1   MEDENG-GGSSSNLDTGKADRSVWLMKCPLLVAKSWQSHPPSDPLPLAKVILSLDPLQSD 60
           MEDE+G GGSSSNLDTGKADRSVWLMKCPLLVAKSWQ+HPPSD LPLAKVILSLDPLQSD
Sbjct: 1   MEDEHGSGGSSSNLDTGKADRSVWLMKCPLLVAKSWQAHPPSDSLPLAKVILSLDPLQSD 60

Query: 61  ESSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPH 120
           ESSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGK+SMEGKVEHKFDMKPH
Sbjct: 61  ESSSLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKVSMEGKVEHKFDMKPH 120

Query: 121 SENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVK 180
           SENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSK+K+KVAPVK
Sbjct: 121 SENLEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKEKKKVAPVK 180

Query: 181 QPEVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTN 240
           Q +VKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTN
Sbjct: 181 QSDVKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTN 240

Query: 241 QGTYELKPEYKKSVEDTGGD 260
           QGTYELKPEYKKSVEDTGG+
Sbjct: 241 QGTYELKPEYKKSVEDTGGE 260

[Close]

BLAST of Carg04019 vs. TAIR 10
Match: AT1G75510.1 (Transcription initiation factor IIF, beta subunit )

HSP 1 Score: 351.7 bits (901), Expect = 5.1e-97
Identity = 180/257 (70.04%), Postives = 207/257 (80.54%), Query Frame = 0


Query: 12  NLDTGKADRSVWLMKCPLLVAKSW--------QSHPPSD-PLPLAKVILSLDPLQSDESS 71
           NLD  K+DRS+WLMKCP++V K+W         S   SD P  +AK++  +DPL+ D  S
Sbjct: 6   NLDIEKSDRSIWLMKCPVVVDKAWHKIAASSSSSFASSDSPPDMAKIVREVDPLRDD--S 65

Query: 72  SLQFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPHSEN 131
             +FKM M G E GN+PK ++LNMF DFVPM  FS+ +QG  + EGKV+HKFDMKP+ E 
Sbjct: 66  PPEFKMYMVGAEYGNMPKCYALNMFTDFVPMGGFSDVNQGCAAAEGKVDHKFDMKPYGET 125

Query: 132 LEMYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVKQPE 191
           +E Y +LCRERT+K+MVKNRQIQVIDNDRGVHMRPMPGM+GL+SS SK+KRK  PVKQ E
Sbjct: 126 IEEYARLCRERTSKAMVKNRQIQVIDNDRGVHMRPMPGMLGLVSSNSKEKRKPPPVKQTE 185

Query: 192 VKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQGT 251
           VKRTRRDRGELE IMFKLFE QPNW LKQLVQETDQPAQFLKEILNELCVYNKRG+NQGT
Sbjct: 186 VKRTRRDRGELEAIMFKLFEGQPNWTLKQLVQETDQPAQFLKEILNELCVYNKRGSNQGT 245

Query: 252 YELKPEYKKSVE-DTGG 259
           YELKPEYKKS E DTGG
Sbjct: 246 YELKPEYKKSAEDDTGG 260

[Close]

BLAST of Carg04019 vs. TAIR 10
Match: AT3G52270.1 (Transcription initiation factor IIF, beta subunit )

HSP 1 Score: 294.7 bits (753), Expect = 7.4e-80
Identity = 149/251 (59.36%), Postives = 195/251 (77.69%), Query Frame = 0


Query: 13  LDTGKADRSVWLMKCPLLVAKSWQSHP-------PSDPL-PLAKVILSLDPLQSDESSSL 72
           L+TG A+RS+ LMK P LVA S QSH        P DP  P AKVIL +DPL + E    
Sbjct: 23  LETGLAERSMLLMKAPSLVASSLQSHSFPDDPYRPDDPYRPDAKVILGVDPL-AHEDEGT 82

Query: 73  QFKMEMAGTETGNVPKSFSLNMFKDFVPMCVFSEASQGKMSMEGKVEHKFDMKPHSENLE 132
           QF ME+A  ++GN+P+ ++L+M KDF+PM VF E+S GKMS+EGK+++KFDM+PH+EN+E
Sbjct: 83  QFVMELARADSGNMPRRYTLDMSKDFIPMNVFCESSDGKMSVEGKIKNKFDMRPHNENIE 142

Query: 133 MYGKLCRERTNKSMVKNRQIQVIDNDRGVHMRPMPGMVGLISSTSKDKRKVAPVKQPEVK 192
            YG+LCRERTNK M KNRQIQVIDN RG+HMRPMPGM+  I + + +K+K+   +  E+K
Sbjct: 143 SYGRLCRERTNKYMGKNRQIQVIDNARGMHMRPMPGMI--IPTAAPEKKKLTN-RTSEMK 202

Query: 193 RTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQGTYE 252
           RTRRDR E+E++MF LFERQ NW L+ L+QETDQP QFLK++L +LC+YN +G+NQGTYE
Sbjct: 203 RTRRDRREMEEVMFNLFERQSNWTLRLLIQETDQPEQFLKDLLKDLCIYNNKGSNQGTYE 262

Query: 253 LKPEYKKSVED 256
           LKPEYKK+ ++
Sbjct: 263 LKPEYKKATQE 269

The following BLAST results are available for this feature:

BLAST of Carg04019 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022951326.1	5.2e-144	100.00	transcription initiation factor IIF subunit beta [Cucurbita moschata] >XP_023537...	[more]
XP_023002475.1	1.5e-143	99.61	transcription initiation factor IIF subunit beta [Cucurbita maxima]	[more]
XP_038884084.1	4.2e-141	97.68	transcription initiation factor IIF subunit beta-like [Benincasa hispida]	[more]
XP_008444765.1	2.5e-138	96.15	PREDICTED: transcription initiation factor IIF subunit beta-like [Cucumis melo] ...	[more]
XP_004152667.1	9.6e-138	96.15	transcription initiation factor IIF subunit beta [Cucumis sativus] >KGN62646.1 h...	[more]

BLAST of Carg04019 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O94424	4.0e-22	32.05	Transcription initiation factor IIF subunit beta OS=Schizosaccharomyces pombe (s...	[more]
Q54KT7	1.1e-16	27.24	General transcription factor IIF subunit 2 OS=Dictyostelium discoideum OX=44689 ...	[more]
P41900	1.1e-16	27.69	General transcription factor IIF subunit 2 OS=Drosophila melanogaster OX=7227 GN...	[more]
Q2T9L9	1.9e-16	28.34	General transcription factor IIF subunit 2 OS=Bos taurus OX=9913 GN=GTF2F2 PE=2 ...	[more]
Q01750	1.9e-16	28.74	General transcription factor IIF subunit 2 OS=Rattus norvegicus OX=10116 GN=Gtf2...	[more]

BLAST of Carg04019 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GH97	2.5e-144	100.00	Transcription initiation factor IIF subunit beta OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1KP21	7.4e-144	99.61	Transcription initiation factor IIF subunit beta OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A5A7VAJ9	1.2e-138	96.15	Transcription initiation factor IIF subunit beta OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3BBV5	1.2e-138	96.15	Transcription initiation factor IIF subunit beta OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A0A0LRL0	4.6e-138	96.15	Transcription initiation factor IIF subunit beta OS=Cucumis sativus OX=3659 GN=C...	[more]

BLAST of Carg04019 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G75510.1	5.1e-97	70.04	Transcription initiation factor IIF, beta subunit	[more]
AT3G52270.1	7.4e-80	59.36	Transcription initiation factor IIF, beta subunit	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 185..252 e-value: 3.9E-35 score: 121.8
IPR040450	TFIIF beta subunit, HTH domain	PFAM	PF02270	TFIIF_beta	coord: 187..250 e-value: 1.2E-20 score: 73.4
IPR040504	TFIIF, beta subunit, N-terminal	PFAM	PF17683	TFIIF_beta_N	coord: 20..134 e-value: 3.4E-6 score: 27.8
IPR003196	Transcription initiation factor IIF, beta subunit	PANTHER	PTHR10445	GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2	coord: 8..255
None	No IPR available	PANTHER	PTHR10445:SF2	TRANSCRIPTION INITIATION FACTOR IIF, BETA SUBUNIT	coord: 8..255
None	No IPR available	CDD	cd07980	TFIIF_beta	coord: 12..118 e-value: 8.95444E-20 score: 80.4488
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 185..251
IPR011039	Transcription Factor IIF, Rap30/Rap74, interaction	SUPERFAMILY	50916	Rap30/74 interaction domains	coord: 10..119

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg04019-RA	Carg04019-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006367	transcription initiation from RNA polymerase II promoter
biological_process	GO:0006413	translational initiation
biological_process	GO:0006366	transcription by RNA polymerase II
cellular_component	GO:0005674	transcription factor TFIIF complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0003743	translation initiation factor activity