Carg03976 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg03976
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	SKP1-like protein
Location	Carg_Chr12: 2357661 .. 2360213 (+)
RNA-Seq Expression	Carg03976
Synteny	Carg03976

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTGTGGTCACCGTTTGGTTTTCGACCGCTTTAGCAAATCGCGACTCTCTCTCTCTTTCTCTTTCTCTTTCTCTCTCTCTCTCTTTGGGTTTCAGACCTCACCGAATCTTCGAGAAAGGAGATACGGCACCATGTCTTCAGGGAGGAAGATCACGTTGAAGAGCTCCGATGGCGAGATCTTTGAGGTTGACGAAGCCGTCGCCTTGGAGTCACAGACGATCAAGCACATGATAGAAGACGATTGCGCCGATAGTGGAATCCCCCTCCCTAACGTCAACAGCAAGATCTTGTCCAAGGTTATCGAGTACTGCAAGAAGCACGTCGAGTCTCCCAAGCCGGAGGATCGTGCTGGCTCCGTCGAGGACGACCTCAAGACTTGGGACAGCGAATTCGTCAAAGTCGACCAGGCGACCTTGTTCGATCTCATTCTGGTATGCGGATGATACTTTCTAAATTTTCTTGCGGTTTCTTGCGGTTTTGTCCTTTTTTTTCCCTCTGTATCTATGCCCTCCGATTGTTCTCTTTTTGTGGCTAATTTCGGGTCTAGGGTTTGGATACTGCTTAGATTTGGGGTAAGGTTCTTTCGGTTCTTGTTTCGTTACGTACCCTATGGTTGCATAATACGTTTTCTGTTTTGGTATGATGCCTGAATCAGATATCTTACATTGTGAATAAGATATTGCAAAATTACTGAAACATGATAGCCGCCTGCTGTTATATGTGACATCTATAGCTATATTTTAGAACGGGAGTCGGTAGGGGAGTTTATTTATTGATTTGTAAGGGCTTTGTATGGGAGGTTCACAAATGTTTGTCATTGAGAAGGCTTTTGATGTATTAGGCGTTTGGCTGCAATCTTTATTCCTTATATAATCTTTGTTTGGAATTGTTACACTAAAACATGCGCGGTATATGTCCTGTTTATCGAGTGATGAAATTCATGTACACCAACAATAGCCATTCCAACTTGTTTTCTGTTTAATCCGATACAATCTTTTATGTTTTGTTTATGGCTGTAACATTTGTTTAGTTGAGGTAATTCGATGTCAATTGCTTATTTTCAATTGGGATATGGGTGAATCTCTTTCCTCTTACCAGTTTATCTTGATCTTTCCTGGTTTTATTGATTTTATTTCTACTTTTGTTCCTTCCTAATAAGATTTAAACGAATTTTTTTATTTACCCGTTTCGAACTTGCTAACGTTTCGAACTTGCTAATCATATGATAATTATTCTTTGTTGTTCATTTATGTGTGTGTGTGACTGTGTGTTTATATTTTATGGTTTATCTGAAATTTACTCGTTCTGTGTTGTATTTAGTTGAATTATTTCATCATCTTTTTTGACAAATGAGTAACTTGAATCCACCTTAAATCCCTTTGGAAGATGTTAATGGTTTTCGTTGAGAGCTTAAAAATTAGGCTTTATAAATTAATGTTGTTTTGTTTTGTAAATTACATCGACAACCATAGTAACTTCATACGGTTGTTCGTTGAGAGAATAGTCGAGGTGTGCACACAAGATGTGCTTGGTATATATTGTGAGACGACACGGTTGACGCTCCCCCAAGTAGAGAGGCATCACTGTAACTTTTTGTTTCTTTTCGTTCCCTTAACTTGTGATATGGGATAATGATGGTCAGAATCTAATCCTACATGACATTTGCAGGCTGCAAACTATCTCAATATCAAGAGCTTGCTAGATTTGACTTGTCAGACGGTGGCTGACATGATCAAGGGGAAGACTCCTGAGGAGATCCGCAAGACATTTAACATCAAGAATGACTTCACACCCGAGGAAGAGGAGGAAGTTCGTAGGGAGAATCAATGGGCGTTCGAGTAAAAAAAAAGAAGTAAACGCTTATATTCTACTTTCTCTTGTCGCATTGTAACATTTGATACTTGTTCTTTCTGTTAAACTAAACTATGTGATGTTCGTTGGGTGTTGTCTTCATTCCTCAATCGCTGTTTTTCATCGAACTTTACCCGACTTGCATTTCTTCATAATATAAGACAGAACTCGTGAAGCACTCGTGTCGCATAATGTAATAGATTGGCTGCTTCATGAATGGTGTCATTGCTGTCAACTCCTCCGGTATTATATTACTTTCCTGTTCAACTCATCGAGACCTGTAGATTTTGAAACCAAACTTCTTTTAGGTGATGGATTTGACTCGTGAGAATAGTCGAGGCGTTAGAACACAGATGGATTAAAAAATTTAAACATAAACCATACTTTTCCCCTTCCACCCAAAGATAGGTATGGATTTTGGGATCATAGGCCAAATATTCCAAACCGAACATAATTGACTTCTTTTTGCATCCCAACAATTTGATAAATGAACACTGCTGCCAACATAACACGTCCAACCCTTCATCAGCTAAATTGTTGTCCGCGAGGAGGCCAACAACTATAAACGCTTGGTGTGAAAAGCTTTGCCAATGGAACTATCAAAATAGGGGTGGGAGGTGGCTATCATGGCAAGCAAATCAGGCCAACACTTCTTGGAACGATGGGCAACCAAAGTTTTGAGGTGTTCAAGGGGGTTTTCA

mRNA sequence

TTTTGTGGTCACCGTTTGGTTTTCGACCGCTTTAGCAAATCGCGACTCTCTCTCTCTTTCTCTTTCTCTTTCTCTCTCTCTCTCTTTGGGTTTCAGACCTCACCGAATCTTCGAGAAAGGAGATACGGCACCATGTCTTCAGGGAGGAAGATCACGTTGAAGAGCTCCGATGGCGAGATCTTTGAGGTTGACGAAGCCGTCGCCTTGGAGTCACAGACGATCAAGCACATGATAGAAGACGATTGCGCCGATAGTGGAATCCCCCTCCCTAACGTCAACAGCAAGATCTTGTCCAAGGTTATCGAGTACTGCAAGAAGCACGTCGAGTCTCCCAAGCCGGAGGATCGTGCTGGCTCCGTCGAGGACGACCTCAAGACTTGGGACAGCGAATTCGTCAAAGTCGACCAGGCGACCTTGTTCGATCTCATTCTGGCTGCAAACTATCTCAATATCAAGAGCTTGCTAGATTTGACTTGTCAGACGGTGGCTGACATGATCAAGGGGAAGACTCCTGAGGAGATCCGCAAGACATTTAACATCAAGAATGACTTCACACCCGAGGAAGAGGAGGAAGTTCGTAGGGAGAATCAATGGGCGTTCGAGTAAAAAAAAAGAAGTAAACGCTTATATTCTACTTTCTCTTGTCGCATTGTAACATTTGATACTTGTTCTTTCTGTTAAACTAAACTATGTGATGTTCGTTGGGTGTTGTCTTCATTCCTCAATCGCTGTTTTTCATCGAACTTTACCCGACTTGCATTTCTTCATAATATAAGACAGAACTCGTGAAGCACTCGTGTCGCATAATGTAATAGATTGGCTGCTTCATGAATGGTGTCATTGCTGTCAACTCCTCCGGTATTATATTACTTTCCTGTTCAACTCATCGAGACCTGTAGATTTTGAAACCAAACTTCTTTTAGGTGATGGATTTGACTCGTGAGAATAGTCGAGGCGTTAGAACACAGATGGATTAAAAAATTTAAACATAAACCATACTTTTCCCCTTCCACCCAAAGATAGGTATGGATTTTGGGATCATAGGCCAAATATTCCAAACCGAACATAATTGACTTCTTTTTGCATCCCAACAATTTGATAAATGAACACTGCTGCCAACATAACACGTCCAACCCTTCATCAGCTAAATTGTTGTCCGCGAGGAGGCCAACAACTATAAACGCTTGGTGTGAAAAGCTTTGCCAATGGAACTATCAAAATAGGGGTGGGAGGTGGCTATCATGGCAAGCAAATCAGGCCAACACTTCTTGGAACGATGGGCAACCAAAGTTTTGAGGTGTTCAAGGGGGTTTTCA

Coding sequence (CDS)

TTTTGTGGTCACCGTTTGGTTTTCGACCGCTTTAGCAAATCGCGACTCTCTCTCTCTTTCTCTTTCTCTTTCTCTCTCTCTCTCTTTGGGTTTCAGACCTCACCGAATCTTCGAGAAAGGAGATACGGCACCATGTCTTCAGGGAGGAAGATCACGTTGAAGAGCTCCGATGGCGAGATCTTTGAGGTTGACGAAGCCGTCGCCTTGGAGTCACAGACGATCAAGCACATGATAGAAGACGATTGCGCCGATAGTGGAATCCCCCTCCCTAACGTCAACAGCAAGATCTTGTCCAAGGTTATCGAGTACTGCAAGAAGCACGTCGAGTCTCCCAAGCCGGAGGATCGTGCTGGCTCCGTCGAGGACGACCTCAAGACTTGGGACAGCGAATTCGTCAAAGTCGACCAGGCGACCTTGTTCGATCTCATTCTGGCTGCAAACTATCTCAATATCAAGAGCTTGCTAGATTTGACTTGTCAGACGGTGGCTGACATGATCAAGGGGAAGACTCCTGAGGAGATCCGCAAGACATTTAACATCAAGAATGACTTCACACCCGAGGAAGAGGAGGAAGTTCGTAGGGAGAATCAATGGGCGTTCGAGTAA

Protein sequence

FCGHRLVFDRFSKSRLSLSFSFSFSLSLFGFQTSPNLRERRYGTMSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYCKKHVESPKPEDRAGSVEDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE

Homology

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BLAST of Carg03976 vs. NCBI nr
Match: KAG7020437.1 (SKP1-like protein 1B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 396.0 bits (1016), Expect = 2.0e-106
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0


Query: 1   FCGHRLVFDRFSKSRLSLSFSFSFSLSLFGFQTSPNLRERRYGTMSSGRKITLKSSDGEI 60
           FCGHRLVFDRFSKSRLSLSFSFSFSLSLFGFQTSPNLRERRYGTMSSGRKITLKSSDGEI
Sbjct: 1   FCGHRLVFDRFSKSRLSLSFSFSFSLSLFGFQTSPNLRERRYGTMSSGRKITLKSSDGEI 60

Query: 61  FEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYCKKHVESPKPEDRAGSV 120
           FEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYCKKHVESPKPEDRAGSV
Sbjct: 61  FEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYCKKHVESPKPEDRAGSV 120

Query: 121 EDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVADMIKGKTPEEIRKTFNI 180
           EDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVADMIKGKTPEEIRKTFNI
Sbjct: 121 EDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVADMIKGKTPEEIRKTFNI 180

Query: 181 KNDFTPEEEEEVRRENQWAFE 202
           KNDFTPEEEEEVRRENQWAFE
Sbjct: 181 KNDFTPEEEEEVRRENQWAFE 201

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BLAST of Carg03976 vs. NCBI nr
Match: KAG6585523.1 (SKP1-like protein 1B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 335.1 bits (858), Expect = 4.1e-88
Identity = 169/169 (100.00%), Postives = 169/169 (100.00%), Query Frame = 0


Query: 33  TSPNLRERRYGTMSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNV 92
           TSPNLRERRYGTMSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNV
Sbjct: 419 TSPNLRERRYGTMSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNV 478

Query: 93  NSKILSKVIEYCKKHVESPKPEDRAGSVEDDLKTWDSEFVKVDQATLFDLILAANYLNIK 152
           NSKILSKVIEYCKKHVESPKPEDRAGSVEDDLKTWDSEFVKVDQATLFDLILAANYLNIK
Sbjct: 479 NSKILSKVIEYCKKHVESPKPEDRAGSVEDDLKTWDSEFVKVDQATLFDLILAANYLNIK 538

Query: 153 SLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 202
           SLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 539 SLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 587

[Close]

BLAST of Carg03976 vs. NCBI nr
Match: KAG7029453.1 (SKP1-like protein 1B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 315.5 bits (807), Expect = 3.4e-82
Identity = 161/184 (87.50%), Postives = 169/184 (91.85%), Query Frame = 0


Query: 20  FSFSFSLSLFGFQTSPN--LRERRYGTMSSGRKITLKSSDGEIFEVDEAVALESQTIKHM 79
           +S  F+    G    P+   ++ +YGTMS+GRKITLKSSDGEIFEVDEAVALESQTIKHM
Sbjct: 423 YSLRFATQRLGLGLRPHRIFQKAKYGTMSAGRKITLKSSDGEIFEVDEAVALESQTIKHM 482

Query: 80  IEDDCADSGIPLPNVNSKILSKVIEYCKKHVESPKPEDRAGSVEDDLKTWDSEFVKVDQA 139
           IEDDCADSGIPLPNVNSKILSKVIEYCKKHVESPKPEDR GSV+DDLKTWDSEFVKVDQA
Sbjct: 483 IEDDCADSGIPLPNVNSKILSKVIEYCKKHVESPKPEDRTGSVDDDLKTWDSEFVKVDQA 542

Query: 140 TLFDLILAANYLNIKSLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQ 199
           TLFDLILAANYLNIKSLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQ
Sbjct: 543 TLFDLILAANYLNIKSLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQ 602

Query: 200 WAFE 202
           WAFE
Sbjct: 603 WAFE 606

[Close]

BLAST of Carg03976 vs. NCBI nr
Match: KAG6598516.1 (SKP1-like protein 1B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 315.5 bits (807), Expect = 3.4e-82
Identity = 161/184 (87.50%), Postives = 169/184 (91.85%), Query Frame = 0


Query: 20  FSFSFSLSLFGFQTSPN--LRERRYGTMSSGRKITLKSSDGEIFEVDEAVALESQTIKHM 79
           +S  F+    G    P+   ++ +YGTMS+GRKITLKSSDGEIFEVDEAVALESQTIKHM
Sbjct: 79  YSLRFATQRLGLGLRPHRIFQKAKYGTMSAGRKITLKSSDGEIFEVDEAVALESQTIKHM 138

Query: 80  IEDDCADSGIPLPNVNSKILSKVIEYCKKHVESPKPEDRAGSVEDDLKTWDSEFVKVDQA 139
           IEDDCADSGIPLPNVNSKILSKVIEYCKKHVESPKPEDR GSV+DDLKTWDSEFVKVDQA
Sbjct: 139 IEDDCADSGIPLPNVNSKILSKVIEYCKKHVESPKPEDRTGSVDDDLKTWDSEFVKVDQA 198

Query: 140 TLFDLILAANYLNIKSLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQ 199
           TLFDLILAANYLNIKSLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQ
Sbjct: 199 TLFDLILAANYLNIKSLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQ 258

Query: 200 WAFE 202
           WAFE
Sbjct: 259 WAFE 262

[Close]

BLAST of Carg03976 vs. NCBI nr
Match: XP_022951749.1 (SKP1-like protein 1B [Cucurbita moschata] >XP_023002536.1 SKP1-like protein 1B [Cucurbita maxima] >XP_023538234.1 SKP1-like protein 1B [Cucurbita pepo subsp. pepo])

HSP 1 Score: 310.5 bits (794), Expect = 1.1e-80
Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 0


Query: 45  MSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYC 104
           MSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYC
Sbjct: 1   MSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYC 60

Query: 105 KKHVESPKPEDRAGSVEDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVAD 164
           KKHVESPKPEDRAGSVEDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVAD
Sbjct: 61  KKHVESPKPEDRAGSVEDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVAD 120

Query: 165 MIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 202
           MIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 121 MIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 157

[Close]

BLAST of Carg03976 vs. ExPASy Swiss-Prot
Match: Q9FHW7 (SKP1-like protein 1B OS=Arabidopsis thaliana OX=3702 GN=SKP1B PE=1 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 3.4e-69
Identity = 135/171 (78.95%), Postives = 146/171 (85.38%), Query Frame = 0


Query: 45  MSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYC 104
           MS+ RKITLKSSDGE FE+DEAVALESQTIKHMIEDDC D+GIPLPNV SKILSKVIEYC
Sbjct: 1   MSTVRKITLKSSDGENFEIDEAVALESQTIKHMIEDDCTDNGIPLPNVTSKILSKVIEYC 60

Query: 105 KKHVESPKPED--------------RAGSVEDDLKTWDSEFVKVDQATLFDLILAANYLN 164
           K+HVE+ +  +               +GS ++DLKTWDSEF+KVDQ TLFDLILAANYLN
Sbjct: 61  KRHVEAAEKSETTADAAAATTTTTVASGSSDEDLKTWDSEFIKVDQGTLFDLILAANYLN 120

Query: 165 IKSLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 202
           IK LLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 121 IKGLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 171

[Close]

BLAST of Carg03976 vs. ExPASy Swiss-Prot
Match: Q6PL11 (SKP1-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=SKP1 PE=1 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 5.1e-65
Identity = 130/166 (78.31%), Postives = 140/166 (84.34%), Query Frame = 0


Query: 49  RKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYCKKHV 108
           + ITLKSSDGE FEV+EAVA+ESQTI+HMIEDDCAD+GIPLPNVNSKILSKVIEYC KHV
Sbjct: 8   KMITLKSSDGEEFEVEEAVAMESQTIRHMIEDDCADNGIPLPNVNSKILSKVIEYCNKHV 67

Query: 109 E-----SPKPEDRAGSVE--------DDLKTWDSEFVKVDQATLFDLILAANYLNIKSLL 168
                 + K  D A S          +DLK WD++FVKVDQATLFDLILAANYLNIK LL
Sbjct: 68  HAAAAAASKAADDAASAAAAVPPPSGEDLKNWDADFVKVDQATLFDLILAANYLNIKGLL 127

Query: 169 DLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 202
           DLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEE+RRENQWAFE
Sbjct: 128 DLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEIRRENQWAFE 173

[Close]

BLAST of Carg03976 vs. ExPASy Swiss-Prot
Match: Q39255 (SKP1-like protein 1A OS=Arabidopsis thaliana OX=3702 GN=SKP1A PE=1 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 6.7e-65
Identity = 123/159 (77.36%), Postives = 141/159 (88.68%), Query Frame = 0


Query: 47  SGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYCKK 106
           S +KI LKSSDGE FEV+EAVALESQTI HM+EDDC D+G+PLPNV SKIL+KVIEYCK+
Sbjct: 2   SAKKIVLKSSDGESFEVEEAVALESQTIAHMVEDDCVDNGVPLPNVTSKILAKVIEYCKR 61

Query: 107 HVESPKPE----DRAGSVEDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTV 166
           HVE+   +    + A + +DDLK WD++F+K+DQATLF+LILAANYLNIK+LLDLTCQTV
Sbjct: 62  HVEAAASKAEAVEGAATSDDDLKAWDADFMKIDQATLFELILAANYLNIKNLLDLTCQTV 121

Query: 167 ADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 202
           ADMIKGKTPEEIR TFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 122 ADMIKGKTPEEIRTTFNIKNDFTPEEEEEVRRENQWAFE 160

[Close]

BLAST of Carg03976 vs. ExPASy Swiss-Prot
Match: Q651E8 (SKP1-like protein 20 OS=Oryza sativa subsp. japonica OX=39947 GN=SKP20 PE=1 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 1.4e-62
Identity = 124/165 (75.15%), Postives = 140/165 (84.85%), Query Frame = 0


Query: 51  ITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYCKKHVE- 110
           ITL+S +G++FEV EAVA+ESQTI+HMIED CAD+GIPLPNV++KILSKVIEYC KHVE 
Sbjct: 11  ITLRSCEGQVFEVAEAVAMESQTIRHMIEDKCADTGIPLPNVSAKILSKVIEYCSKHVEA 70

Query: 111 -------------SPKPEDRAGSVEDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLD 170
                        +P   +   +VED+LKT+D+EFVKVDQ+TLFDLILAANYLNIK LLD
Sbjct: 71  RGGAAAAADGDAPAPAAVEANKAVEDELKTFDAEFVKVDQSTLFDLILAANYLNIKGLLD 130

Query: 171 LTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 202
           LTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 131 LTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 175

[Close]

BLAST of Carg03976 vs. ExPASy Swiss-Prot
Match: Q9LNT9 (SKP1-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=ASK4 PE=1 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 6.7e-57
Identity = 114/158 (72.15%), Postives = 131/158 (82.91%), Query Frame = 0


Query: 49  RKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYCKKHV 108
           + I LKSSDGE FE++EAVA++SQTIKHMIEDDCAD+GIPLPNV   IL+KVIEYCKKHV
Sbjct: 6   KMIILKSSDGESFEIEEAVAVKSQTIKHMIEDDCADNGIPLPNVTGAILAKVIEYCKKHV 65

Query: 109 ESPK----PEDRAGSVE-DDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVA 168
           E+       +D  GS E D+LK WDSEFVKVDQ TLFDLILAANYLNI  LLDLTC+ VA
Sbjct: 66  EAAAEAGGDKDFYGSAENDELKNWDSEFVKVDQPTLFDLILAANYLNIGGLLDLTCKAVA 125

Query: 169 DMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 202
           D ++GKTPE++R  FNIKND+TPEEE EVR EN+WAFE
Sbjct: 126 DQMRGKTPEQMRAHFNIKNDYTPEEEAEVRNENKWAFE 163

[Close]

BLAST of Carg03976 vs. ExPASy TrEMBL
Match: A0A6J1KLK5 (SKP1-like protein OS=Cucurbita maxima OX=3661 GN=LOC111496349 PE=3 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 5.3e-81
Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 0


Query: 45  MSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYC 104
           MSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYC
Sbjct: 1   MSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYC 60

Query: 105 KKHVESPKPEDRAGSVEDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVAD 164
           KKHVESPKPEDRAGSVEDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVAD
Sbjct: 61  KKHVESPKPEDRAGSVEDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVAD 120

Query: 165 MIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 202
           MIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 121 MIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 157

[Close]

BLAST of Carg03976 vs. ExPASy TrEMBL
Match: A0A6J1GJP4 (SKP1-like protein OS=Cucurbita moschata OX=3662 GN=LOC111454489 PE=3 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 5.3e-81
Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 0


Query: 45  MSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYC 104
           MSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYC
Sbjct: 1   MSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYC 60

Query: 105 KKHVESPKPEDRAGSVEDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVAD 164
           KKHVESPKPEDRAGSVEDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVAD
Sbjct: 61  KKHVESPKPEDRAGSVEDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVAD 120

Query: 165 MIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 202
           MIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 121 MIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 157

[Close]

BLAST of Carg03976 vs. ExPASy TrEMBL
Match: A0A6J1K9X2 (SKP1-like protein OS=Cucurbita maxima OX=3661 GN=LOC111491993 PE=3 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 3.4e-80
Identity = 155/157 (98.73%), Postives = 156/157 (99.36%), Query Frame = 0


Query: 45  MSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYC 104
           MSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYC
Sbjct: 1   MSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYC 60

Query: 105 KKHVESPKPEDRAGSVEDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVAD 164
           KKHVESPKPEDR GSV+DDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVAD
Sbjct: 61  KKHVESPKPEDRTGSVDDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVAD 120

Query: 165 MIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 202
           MIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 121 MIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 157

[Close]

BLAST of Carg03976 vs. ExPASy TrEMBL
Match: A0A0A0LMK0 (SKP1-like protein OS=Cucumis sativus OX=3659 GN=Csa_2G346050 PE=3 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 3.4e-80
Identity = 155/157 (98.73%), Postives = 156/157 (99.36%), Query Frame = 0


Query: 45  MSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYC 104
           MSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYC
Sbjct: 1   MSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYC 60

Query: 105 KKHVESPKPEDRAGSVEDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVAD 164
           KKHVESPKPEDR GSV+DDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVAD
Sbjct: 61  KKHVESPKPEDRTGSVDDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVAD 120

Query: 165 MIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 202
           MIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 121 MIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 157

[Close]

BLAST of Carg03976 vs. ExPASy TrEMBL
Match: A0A6J1HGN0 (SKP1-like protein OS=Cucurbita moschata OX=3662 GN=LOC111462785 PE=3 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 3.4e-80
Identity = 155/157 (98.73%), Postives = 156/157 (99.36%), Query Frame = 0


Query: 45  MSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYC 104
           MSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYC
Sbjct: 1   MSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYC 60

Query: 105 KKHVESPKPEDRAGSVEDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVAD 164
           KKHVESPKPEDR GSV+DDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVAD
Sbjct: 61  KKHVESPKPEDRTGSVDDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVAD 120

Query: 165 MIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 202
           MIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 121 MIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 157

[Close]

BLAST of Carg03976 vs. TAIR 10
Match: AT5G42190.1 (E3 ubiquitin ligase SCF complex subunit SKP1/ASK1 family protein )

HSP 1 Score: 262.7 bits (670), Expect = 2.4e-70
Identity = 135/171 (78.95%), Postives = 146/171 (85.38%), Query Frame = 0


Query: 45  MSSGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYC 104
           MS+ RKITLKSSDGE FE+DEAVALESQTIKHMIEDDC D+GIPLPNV SKILSKVIEYC
Sbjct: 1   MSTVRKITLKSSDGENFEIDEAVALESQTIKHMIEDDCTDNGIPLPNVTSKILSKVIEYC 60

Query: 105 KKHVESPKPED--------------RAGSVEDDLKTWDSEFVKVDQATLFDLILAANYLN 164
           K+HVE+ +  +               +GS ++DLKTWDSEF+KVDQ TLFDLILAANYLN
Sbjct: 61  KRHVEAAEKSETTADAAAATTTTTVASGSSDEDLKTWDSEFIKVDQGTLFDLILAANYLN 120

Query: 165 IKSLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 202
           IK LLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 121 IKGLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 171

[Close]

BLAST of Carg03976 vs. TAIR 10
Match: AT1G75950.1 (S phase kinase-associated protein 1 )

HSP 1 Score: 248.4 bits (633), Expect = 4.7e-66
Identity = 123/159 (77.36%), Postives = 141/159 (88.68%), Query Frame = 0


Query: 47  SGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYCKK 106
           S +KI LKSSDGE FEV+EAVALESQTI HM+EDDC D+G+PLPNV SKIL+KVIEYCK+
Sbjct: 2   SAKKIVLKSSDGESFEVEEAVALESQTIAHMVEDDCVDNGVPLPNVTSKILAKVIEYCKR 61

Query: 107 HVESPKPE----DRAGSVEDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTV 166
           HVE+   +    + A + +DDLK WD++F+K+DQATLF+LILAANYLNIK+LLDLTCQTV
Sbjct: 62  HVEAAASKAEAVEGAATSDDDLKAWDADFMKIDQATLFELILAANYLNIKNLLDLTCQTV 121

Query: 167 ADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 202
           ADMIKGKTPEEIR TFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 122 ADMIKGKTPEEIRTTFNIKNDFTPEEEEEVRRENQWAFE 160

[Close]

BLAST of Carg03976 vs. TAIR 10
Match: AT1G20140.1 (SKP1-like 4 )

HSP 1 Score: 221.9 bits (564), Expect = 4.8e-58
Identity = 114/158 (72.15%), Postives = 131/158 (82.91%), Query Frame = 0


Query: 49  RKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYCKKHV 108
           + I LKSSDGE FE++EAVA++SQTIKHMIEDDCAD+GIPLPNV   IL+KVIEYCKKHV
Sbjct: 6   KMIILKSSDGESFEIEEAVAVKSQTIKHMIEDDCADNGIPLPNVTGAILAKVIEYCKKHV 65

Query: 109 ESPK----PEDRAGSVE-DDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVA 168
           E+       +D  GS E D+LK WDSEFVKVDQ TLFDLILAANYLNI  LLDLTC+ VA
Sbjct: 66  EAAAEAGGDKDFYGSAENDELKNWDSEFVKVDQPTLFDLILAANYLNIGGLLDLTCKAVA 125

Query: 169 DMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 202
           D ++GKTPE++R  FNIKND+TPEEE EVR EN+WAFE
Sbjct: 126 DQMRGKTPEQMRAHFNIKNDYTPEEEAEVRNENKWAFE 163

[Close]

BLAST of Carg03976 vs. TAIR 10
Match: AT4G34210.1 (SKP1-like 11 )

HSP 1 Score: 215.3 bits (547), Expect = 4.5e-56
Identity = 110/156 (70.51%), Postives = 127/156 (81.41%), Query Frame = 0


Query: 47  SGRKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYCKK 106
           S + I L SSDG+ FEV+EAVA++SQTI HM+EDDC   GIPL NV SKIL KVIEYCKK
Sbjct: 2   SSKMIVLMSSDGQSFEVEEAVAIQSQTIAHMVEDDCVADGIPLANVESKILVKVIEYCKK 61

Query: 107 -HVESPKPEDRAGSVEDDLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVADM 166
            HV+   P       E+DL  WD +F+ ++Q+T+F+LILAANYLNIKSLLDLTCQTVADM
Sbjct: 62  HHVDEANPIS-----EEDLNNWDEKFMDLEQSTIFELILAANYLNIKSLLDLTCQTVADM 121

Query: 167 IKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 202
           IKGKTPEEIR TFNI+NDFTPEEEE VR+ENQWAFE
Sbjct: 122 IKGKTPEEIRSTFNIENDFTPEEEEAVRKENQWAFE 152

[Close]

BLAST of Carg03976 vs. TAIR 10
Match: AT2G25700.1 (SKP1-like 3 )

HSP 1 Score: 214.2 bits (544), Expect = 9.9e-56
Identity = 109/158 (68.99%), Postives = 129/158 (81.65%), Query Frame = 0


Query: 49  RKITLKSSDGEIFEVDEAVALESQTIKHMIEDDCADSGIPLPNVNSKILSKVIEYCKKHV 108
           + I LKSSDGE FEV+EAVA+ESQTIKHMIEDDC D+GIPLPNV   IL+KVIEYCKKHV
Sbjct: 6   KMIILKSSDGESFEVEEAVAVESQTIKHMIEDDCVDNGIPLPNVTGAILAKVIEYCKKHV 65

Query: 109 ESPK----PEDRAGSVED-DLKTWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVA 168
           E+       +D  GS E+ +LKTWD++FVKVD  TLFDL+ AANYLNI  LLDLTC+ VA
Sbjct: 66  EAAAEAGGDKDFYGSTENHELKTWDNDFVKVDHPTLFDLLRAANYLNISGLLDLTCKAVA 125

Query: 169 DMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 202
           D ++GKTP ++R+ FNIKND+TPEEE EVR EN+WAFE
Sbjct: 126 DQMRGKTPAQMREHFNIKNDYTPEEEAEVRNENRWAFE 163

The following BLAST results are available for this feature:

BLAST of Carg03976 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7020437.1	2.0e-106	100.00	SKP1-like protein 1B, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6585523.1	4.1e-88	100.00	SKP1-like protein 1B, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7029453.1	3.4e-82	87.50	SKP1-like protein 1B [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6598516.1	3.4e-82	87.50	SKP1-like protein 1B, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022951749.1	1.1e-80	100.00	SKP1-like protein 1B [Cucurbita moschata] >XP_023002536.1 SKP1-like protein 1B [...	[more]

BLAST of Carg03976 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FHW7	3.4e-69	78.95	SKP1-like protein 1B OS=Arabidopsis thaliana OX=3702 GN=SKP1B PE=1 SV=1	[more]
Q6PL11	5.1e-65	78.31	SKP1-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=SKP1 PE=1 SV=1	[more]
Q39255	6.7e-65	77.36	SKP1-like protein 1A OS=Arabidopsis thaliana OX=3702 GN=SKP1A PE=1 SV=1	[more]
Q651E8	1.4e-62	75.15	SKP1-like protein 20 OS=Oryza sativa subsp. japonica OX=39947 GN=SKP20 PE=1 SV=1	[more]
Q9LNT9	6.7e-57	72.15	SKP1-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=ASK4 PE=1 SV=1	[more]

BLAST of Carg03976 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KLK5	5.3e-81	100.00	SKP1-like protein OS=Cucurbita maxima OX=3661 GN=LOC111496349 PE=3 SV=1	[more]
A0A6J1GJP4	5.3e-81	100.00	SKP1-like protein OS=Cucurbita moschata OX=3662 GN=LOC111454489 PE=3 SV=1	[more]
A0A6J1K9X2	3.4e-80	98.73	SKP1-like protein OS=Cucurbita maxima OX=3661 GN=LOC111491993 PE=3 SV=1	[more]
A0A0A0LMK0	3.4e-80	98.73	SKP1-like protein OS=Cucumis sativus OX=3659 GN=Csa_2G346050 PE=3 SV=1	[more]
A0A6J1HGN0	3.4e-80	98.73	SKP1-like protein OS=Cucurbita moschata OX=3662 GN=LOC111462785 PE=3 SV=1	[more]

BLAST of Carg03976 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42190.1	2.4e-70	78.95	E3 ubiquitin ligase SCF complex subunit SKP1/ASK1 family protein	[more]
AT1G75950.1	4.7e-66	77.36	S phase kinase-associated protein 1	[more]
AT1G20140.1	4.8e-58	72.15	SKP1-like 4	[more]
AT4G34210.1	4.5e-56	70.51	SKP1-like 11	[more]
AT2G25700.1	9.9e-56	68.99	SKP1-like 3	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001232	S-phase kinase-associated protein 1-like	SMART	SM00512	skp1_3	coord: 48..151 e-value: 2.4E-55 score: 199.8
IPR011333	SKP1/BTB/POZ domain superfamily	GENE3D	3.30.710.10	Potassium Channel Kv1.1; Chain A	coord: 44..201 e-value: 1.3E-76 score: 258.3
IPR011333	SKP1/BTB/POZ domain superfamily	SUPERFAMILY	54695	POZ domain	coord: 49..111
IPR016072	SKP1 component, dimerisation	PFAM	PF01466	Skp1	coord: 152..199 e-value: 3.1E-30 score: 104.0
IPR016073	SKP1 component, POZ domain	PFAM	PF03931	Skp1_POZ	coord: 50..108 e-value: 6.3E-31 score: 106.3
None	No IPR available	PANTHER	PTHR11165:SF145	PUTATIVE-RELATED	coord: 45..201
None	No IPR available	CDD	cd18322	BTB_POZ_SKP1	coord: 50..166 e-value: 3.23374E-68 score: 202.437
IPR016897	S-phase kinase-associated protein 1	PANTHER	PTHR11165	SKP1	coord: 45..201
IPR036296	SKP1-like, dimerisation domain superfamily	SUPERFAMILY	81382	Skp1 dimerisation domain-like	coord: 124..199

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg03976-RA	Carg03976-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0031146	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0097602	cullin family protein binding