Carg03865 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg03865
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionGamma-glutamylcyclotransferase
LocationCarg_Chr12: 1735695 .. 1738586 (-)
RNA-Seq ExpressionCarg03865
SyntenyCarg03865
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAACGCCCTAATTTTTGTTCTTTTCAGCTAAACGAAAATTCGTTTAGCCTTTTCGTTTCTCTCTTGCTTCTGTAATTTTCGTTTCGGAGCTCTCTCAAGCGGATCAGAGGAATTCTCTTGTTTAAATTTGTCGGTGTTCTTGTAAGAATCCGTTCCATTTTTTTTTTCTTCTACGTTTGCGTTTTTCAAATCGAATGATTTAACTATTCCGCATGAATCTACTTTTGCTGCTTTTGATGTGGATTTGACAATTCCGCTGTCTCTCGTTTCTTTACGATTTTATTCTGGCGAGAAAGGAATTCGAGAAATTGAGGAGTTTATGTTTACATACGAATGAATTTTTTTTGATTTCTTCGAGCGATGACTAAATTAATAGAACAGAGGACGAAGTGATGGATATGCATTCATTGGCCTTAGTTGATTAATTTTAGGGGATATTGTTCTTGCAGATATCGATTCAATCATGGTTCTTTGGGTTTTTGGCTATGGATCTCTTGTGTGGAATCCAGGATTTGACTTCGACGAGAGAGTGATAGGTTTTATTAGAGATTATAGGCGTGTATTCGACCTCGGTAAATTTCTTCCTCAACATTCCAATTCGTTAATCGAAACTAAAGTTTTTCTAATATTTAATTTCCCATTGCACAGCTTGTTTTGATCATAGAGGTACACCACAAAACCCTGCTAGAACTCTCACGCTTGAACCCAAAGAAGGATCCATTTGCGTAAGTTTTGAGCAACTATCATTGTTCATTCATTTGCATTTCCATGGCTGATACAAATTTCATTTTCTATAATAGCAAATTTTTGTAACCTTGCCTGATTATAGCACTCTTTCTCTATACCAGTGGGGTGTTGCATATTGCATCCATGGAGGATCTGAACGAGAGAGAGCGGTTGTGGAGGTATTTCCCCCAAATTTATCTTGATTTTACTTGAATCTATTCCATTTTTGGGAGGGAGTCCCACTTTGGCTAATCTAGGGAATGATCATGAGTTTATAAGTAAGAAACACATCTATGTTGGAACGAGGCTTTTGAGAAAGCCCACATGAGAGCTTATTCTCAAAGTGGACAATATCATATCATTATGGAAAGTCGAGTCGTGGTTTCTAACATTTCCTACGGTTCTTGTTTGGTCCGCAATGTAGTCCCTGGAGAGAAGAGAGTGCGAGTATGATGGAAAGACCATTGTAGACTTTTACAAGGTAAGGATTTGCTTCGTTATTAGGTGTGTTTCTGTTCCATCATTTCCAATTTGGTTTCTTATTTTGATTCCATTTCCATTGTTGATTCAGGATAAAGACTCCATAGAACCAACATTGACAGGAGTTTTAGTGTAAGCTTCTGGATGGATTTTTTCTTCTTAAACATCTGATTTGTTCTAAATCTTAATTGGGATAAGTTGAAAAAGAGCATCTCTGTTCTTGATCTGATTAAGTTTTGCATTCATGAACGCAGTTTTACATCGACACTTGATAAATTGCTAAACAAATACTATCTAGGACCTGCTCCCTTGGAGGAAATGGCTAGGTAATGTCAGGCTTACCATTTGGCTTTCAATGTTCTATTGCATCCCTGAATGGGTTCATTATTGTGACGTCCCACATTGGTTGGGGAGGAGAACAAAACACCATTTATAAGGGTGTGGAAACCTTCCCCTAGCAAGACGGGTGCTTTTTAAAGTCTTGAGGCGAAGCCTGAAATGGAAAGCACAAAGAGAACAATATCTGCTAGCGGTGGATCTGGGCTGTTACAAATGGTATCAGAGCCAGACACCGGACGATGTGCTAGCTTTTTCGCTGTTCCTCGAAGGGGGGTAGACACGAGACGGTGTGCTAGTAAGGATGCTGGGCCTCGAAGGGGAGTGGATTTGATGGTGGTCCCACATCGATTGAAGGAAGGAAAGAGTGCCAGCGAGGACGCTGGGCCCCGAAGAGGGTGGATTGTGATGTCCCATCTTGGTTGGGGGAGGAGAACAAAACACCCTTTATAAGGGGTTCCCTAGCAGACCGTTTTATAGCCTTGAGGGGAAGCCCGAAAGGGAAAGCTCAAAGAGAACAATATCTGCTAGCGGTGGATCTTGGCCCGTTAGAATTCTAAATCTTTCATTTACTAACTTTTCAGCCAAATTGCAACTGCATCTGGTCCATGTGGGAACAACAGAGACTATCTATTCCGATTGGAGAACGCCTTGTTCGAAATTGGTAATCGTCTAACTCTTCGTCCAAAATTGAATTCTGATGTGATTGTATGAACTAAATTTGAGTTGATATTAACTTTACACATCGCCAATTAGGTCACGGAGACGATATGGTGATCGAGCTAGCGAACGAGGTGAGGAATATCCTTGGGAAGAAGGTTAGAAAGGGGAAGAAGAAGCAGGCGGTCAGACCGACGACTCACCTGCCTTTTTTGTCCTTCATCTCCGGTGTTCAGGTGCATCCGCTCGCCGACGCTGCGGTGGGAACGGATTCCTACGAAGAAAATGTAAAGATTGGTATGGTGAAAGGTGAAAGCCATGGTAACTCAAGAGCTAAGAAAACAAGCAATCTGGTGGATTGATGATTGCCACTGTTCTTTTCCCATTAGGTTGATTAGTGAAATTTTGGGGATATTCTTATTGGATTCATGTTTCTGATCTGACCCAATAGAAAGTTGTTGGAAGTTCTTTTTTCACACTTTTCACGTCCTCCATTAATGGAAGTTCTTCACCATTTGGAATTACTTAGGGCATTAGGTTGAATTCATGTTGTTGGGTTGAATGGCAACATGTTTACAAGTTTGGAAGGAAAAATGGTCACTCAACCTTCCGTACAATCTTTGAAGAAAAATGTTTCTATGTCACGTTAAAGAATAATCAATGTAATGGTCCAAATCCATCGCTAGTCG

mRNA sequence

GAAACGCCCTAATTTTTGTTCTTTTCAGCTAAACGAAAATTCGTTTAGCCTTTTCGTTTCTCTCTTGCTTCTGTAATTTTCGTTTCGGAGCTCTCTCAAGCGGATCAGAGGAATTCTCTTGTTTAAATTTGTCGGTGTTCTTATATCGATTCAATCATGGTTCTTTGGGTTTTTGGCTATGGATCTCTTGTGTGGAATCCAGGATTTGACTTCGACGAGAGAGTGATAGGTTTTATTAGAGATTATAGGCGTGTATTCGACCTCGCTTGTTTTGATCATAGAGGTACACCACAAAACCCTGCTAGAACTCTCACGCTTGAACCCAAAGAAGGATCCATTTGCCAAATTTTTGTAACCTTGCCTGATTATAGCACTCTTTCTCTATACCAGTGGGGTGTTGCATATTGCATCCATGGAGGATCTGAACGAGAGAGAGCGGTTGTGGAGTCCCTGGAGAGAAGAGAGTGCGAGTATGATGGAAAGACCATTGTAGACTTTTACAAGGATAAAGACTCCATAGAACCAACATTGACAGGAGTTTTAGTTTTTACATCGACACTTGATAAATTGCTAAACAAATACTATCTAGGACCTGCTCCCTTGGAGGAAATGGCTAGTCTTGAGGCGAAGCCTGAAATGGAAAGCACAAAGAGAACAATATCTGCTAGCGGTGGATCTGGGCTGTTACAAATGGTATCAGAGCCAGACACCGGACGATGTGCTAGCTTTTTCGCTGTTCCTCGAAGGGGGGTAGACACGAGACGGTGTGCTAGTAAGGATGCTGGGCCTCGAAGGGGAGTGGATTTGATGGTGGTCCCACATCGATTGAAGGAAGGAAAGAGTGCCAGCGAGGACGCTGGGCCCCGAAGAGGCCAAATTGCAACTGCATCTGGTCCATGTGGGAACAACAGAGACTATCTATTCCGATTGGAGAACGCCTTGTTCGAAATTGGTCACGGAGACGATATGGTGATCGAGCTAGCGAACGAGGTGAGGAATATCCTTGGGAAGAAGGTTAGAAAGGGGAAGAAGAAGCAGGCGGTCAGACCGACGACTCACCTGCCTTTTTTGTCCTTCATCTCCGGTGTTCAGGTGCATCCGCTCGCCGACGCTGCGGTGGGAACGGATTCCTACGAAGAAAATGTAAAGATTGGTATGGTGAAAGGTGAAAGCCATGGTAACTCAAGAGCTAAGAAAACAAGCAATCTGGTGGATTGATGATTGCCACTGTTCTTTTCCCATTAGGTTGATTAGTGAAATTTTGGGGATATTCTTATTGGATTCATGTTTCTGATCTGACCCAATAGAAAGTTGTTGGAAGTTCTTTTTTCACACTTTTCACGTCCTCCATTAATGGAAGTTCTTCACCATTTGGAATTACTTAGGGCATTAGGTTGAATTCATGTTGTTGGGTTGAATGGCAACATGTTTACAAGTTTGGAAGGAAAAATGGTCACTCAACCTTCCGTACAATCTTTGAAGAAAAATGTTTCTATGTCACGTTAAAGAATAATCAATGTAATGGTCCAAATCCATCGCTAGTCG

Coding sequence (CDS)

ATGGTTCTTTGGGTTTTTGGCTATGGATCTCTTGTGTGGAATCCAGGATTTGACTTCGACGAGAGAGTGATAGGTTTTATTAGAGATTATAGGCGTGTATTCGACCTCGCTTGTTTTGATCATAGAGGTACACCACAAAACCCTGCTAGAACTCTCACGCTTGAACCCAAAGAAGGATCCATTTGCCAAATTTTTGTAACCTTGCCTGATTATAGCACTCTTTCTCTATACCAGTGGGGTGTTGCATATTGCATCCATGGAGGATCTGAACGAGAGAGAGCGGTTGTGGAGTCCCTGGAGAGAAGAGAGTGCGAGTATGATGGAAAGACCATTGTAGACTTTTACAAGGATAAAGACTCCATAGAACCAACATTGACAGGAGTTTTAGTTTTTACATCGACACTTGATAAATTGCTAAACAAATACTATCTAGGACCTGCTCCCTTGGAGGAAATGGCTAGTCTTGAGGCGAAGCCTGAAATGGAAAGCACAAAGAGAACAATATCTGCTAGCGGTGGATCTGGGCTGTTACAAATGGTATCAGAGCCAGACACCGGACGATGTGCTAGCTTTTTCGCTGTTCCTCGAAGGGGGGTAGACACGAGACGGTGTGCTAGTAAGGATGCTGGGCCTCGAAGGGGAGTGGATTTGATGGTGGTCCCACATCGATTGAAGGAAGGAAAGAGTGCCAGCGAGGACGCTGGGCCCCGAAGAGGCCAAATTGCAACTGCATCTGGTCCATGTGGGAACAACAGAGACTATCTATTCCGATTGGAGAACGCCTTGTTCGAAATTGGTCACGGAGACGATATGGTGATCGAGCTAGCGAACGAGGTGAGGAATATCCTTGGGAAGAAGGTTAGAAAGGGGAAGAAGAAGCAGGCGGTCAGACCGACGACTCACCTGCCTTTTTTGTCCTTCATCTCCGGTGTTCAGGTGCATCCGCTCGCCGACGCTGCGGTGGGAACGGATTCCTACGAAGAAAATGTAAAGATTGGTATGGTGAAAGGTGAAAGCCATGGTAACTCAAGAGCTAAGAAAACAAGCAATCTGGTGGATTGA

Protein sequence

MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGSICQIFVTLPDYSTLSLYQWGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDSIEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMASLEAKPEMESTKRTISASGGSGLLQMVSEPDTGRCASFFAVPRRGVDTRRCASKDAGPRRGVDLMVVPHRLKEGKSASEDAGPRRGQIATASGPCGNNRDYLFRLENALFEIGHGDDMVIELANEVRNILGKKVRKGKKKQAVRPTTHLPFLSFISGVQVHPLADAAVGTDSYEENVKIGMVKGESHGNSRAKKTSNLVD
Homology
BLAST of Carg03865 vs. NCBI nr
Match: KAG7020326.1 (Gamma-glutamylcyclotransferase 2-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 719.9 bits (1857), Expect = 1.0e-203
Identity = 353/353 (100.00%), Postives = 353/353 (100.00%), Query Frame = 0

Query: 1   MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS 60
           MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS
Sbjct: 1   MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS 60

Query: 61  ICQIFVTLPDYSTLSLYQWGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDS 120
           ICQIFVTLPDYSTLSLYQWGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDS
Sbjct: 61  ICQIFVTLPDYSTLSLYQWGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDS 120

Query: 121 IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMASLEAKPEMESTKRTISASGGSGLLQMV 180
           IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMASLEAKPEMESTKRTISASGGSGLLQMV
Sbjct: 121 IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMASLEAKPEMESTKRTISASGGSGLLQMV 180

Query: 181 SEPDTGRCASFFAVPRRGVDTRRCASKDAGPRRGVDLMVVPHRLKEGKSASEDAGPRRGQ 240
           SEPDTGRCASFFAVPRRGVDTRRCASKDAGPRRGVDLMVVPHRLKEGKSASEDAGPRRGQ
Sbjct: 181 SEPDTGRCASFFAVPRRGVDTRRCASKDAGPRRGVDLMVVPHRLKEGKSASEDAGPRRGQ 240

Query: 241 IATASGPCGNNRDYLFRLENALFEIGHGDDMVIELANEVRNILGKKVRKGKKKQAVRPTT 300
           IATASGPCGNNRDYLFRLENALFEIGHGDDMVIELANEVRNILGKKVRKGKKKQAVRPTT
Sbjct: 241 IATASGPCGNNRDYLFRLENALFEIGHGDDMVIELANEVRNILGKKVRKGKKKQAVRPTT 300

Query: 301 HLPFLSFISGVQVHPLADAAVGTDSYEENVKIGMVKGESHGNSRAKKTSNLVD 354
           HLPFLSFISGVQVHPLADAAVGTDSYEENVKIGMVKGESHGNSRAKKTSNLVD
Sbjct: 301 HLPFLSFISGVQVHPLADAAVGTDSYEENVKIGMVKGESHGNSRAKKTSNLVD 353

BLAST of Carg03865 vs. NCBI nr
Match: XP_022951435.1 (gamma-glutamylcyclotransferase 2-1-like [Cucurbita moschata])

HSP 1 Score: 462.6 bits (1189), Expect = 3.0e-126
Identity = 247/353 (69.97%), Postives = 248/353 (70.25%), Query Frame = 0

Query: 1   MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS 60
           MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS
Sbjct: 1   MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS 60

Query: 61  ICQIFVTLPDYSTLSLYQWGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDS 120
           IC                WGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDS
Sbjct: 61  IC----------------WGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDS 120

Query: 121 IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMASLEAKPEMESTKRTISASGGSGLLQMV 180
           IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMAS                          
Sbjct: 121 IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMAS-------------------------- 180

Query: 181 SEPDTGRCASFFAVPRRGVDTRRCASKDAGPRRGVDLMVVPHRLKEGKSASEDAGPRRGQ 240
                                                                      Q
Sbjct: 181 -----------------------------------------------------------Q 240

Query: 241 IATASGPCGNNRDYLFRLENALFEIGHGDDMVIELANEVRNILGKKVRKGKKKQAVRPTT 300
           IATASGPCGNNRDYLFRLENALFEIGH DDMVIELANEVRNILGKKVRKGKKKQ VRPTT
Sbjct: 241 IATASGPCGNNRDYLFRLENALFEIGHEDDMVIELANEVRNILGKKVRKGKKKQPVRPTT 252

Query: 301 HLPFLSFISGVQVHPLADAAVGTDSYEENVKIGMVKGESHGNSRAKKTSNLVD 354
           HLPFLSFISGVQVHPLADAAV TDSYEEN KIGM+KGESHGNSRAKKTSNLVD
Sbjct: 301 HLPFLSFISGVQVHPLADAAVATDSYEENGKIGMMKGESHGNSRAKKTSNLVD 252

BLAST of Carg03865 vs. NCBI nr
Match: XP_023537463.1 (gamma-glutamylcyclotransferase 2-1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 458.8 bits (1179), Expect = 4.4e-125
Identity = 245/351 (69.80%), Postives = 246/351 (70.09%), Query Frame = 0

Query: 1   MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS 60
           MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS
Sbjct: 1   MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS 60

Query: 61  ICQIFVTLPDYSTLSLYQWGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDS 120
           IC                WGVAYCIHGGSERERAV+E LERRECEYDGKTIVDFYKDKDS
Sbjct: 61  IC----------------WGVAYCIHGGSERERAVLECLERRECEYDGKTIVDFYKDKDS 120

Query: 121 IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMASLEAKPEMESTKRTISASGGSGLLQMV 180
           IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMAS                          
Sbjct: 121 IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMAS-------------------------- 180

Query: 181 SEPDTGRCASFFAVPRRGVDTRRCASKDAGPRRGVDLMVVPHRLKEGKSASEDAGPRRGQ 240
                                                                      Q
Sbjct: 181 -----------------------------------------------------------Q 240

Query: 241 IATASGPCGNNRDYLFRLENALFEIGHGDDMVIELANEVRNILGKKVRKGKKKQAVRPTT 300
           IATASGPCGNNRDYLFRLENALFEIGH DDMVIELANEVRNILGKKVRKGKKKQAVRPTT
Sbjct: 241 IATASGPCGNNRDYLFRLENALFEIGHEDDMVIELANEVRNILGKKVRKGKKKQAVRPTT 250

Query: 301 HLPFLSFISGVQVHPLADAAVGTDSYEENVKIGMVKGESHGNSRAKKTSNL 352
           HLPFLSFISGVQVHPLADAAV TDSYEEN KIGMVKGESHGNSRAKKTSNL
Sbjct: 301 HLPFLSFISGVQVHPLADAAVATDSYEENGKIGMVKGESHGNSRAKKTSNL 250

BLAST of Carg03865 vs. NCBI nr
Match: XP_023002479.1 (gamma-glutamylcyclotransferase 2-1-like [Cucurbita maxima])

HSP 1 Score: 439.5 bits (1129), Expect = 2.7e-119
Identity = 237/351 (67.52%), Postives = 240/351 (68.38%), Query Frame = 0

Query: 1   MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS 60
           MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS
Sbjct: 1   MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS 60

Query: 61  ICQIFVTLPDYSTLSLYQWGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDS 120
           IC                WGVAYCIHGGSERERA +E LERRECEYDGKTIV+FYKDKDS
Sbjct: 61  IC----------------WGVAYCIHGGSERERAAMECLERRECEYDGKTIVNFYKDKDS 120

Query: 121 IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMASLEAKPEMESTKRTISASGGSGLLQMV 180
           IEPTLTGVLVFTSTLDKLLNKYYLG A LEEMA                           
Sbjct: 121 IEPTLTGVLVFTSTLDKLLNKYYLGHATLEEMAC-------------------------- 180

Query: 181 SEPDTGRCASFFAVPRRGVDTRRCASKDAGPRRGVDLMVVPHRLKEGKSASEDAGPRRGQ 240
                                                                      Q
Sbjct: 181 -----------------------------------------------------------Q 240

Query: 241 IATASGPCGNNRDYLFRLENALFEIGHGDDMVIELANEVRNILGKKVRKGKKKQAVRPTT 300
           IATASGPCGNNR+YLFRLENALFEIGH DDMVIELANEVRNILGKKVRKGKKKQAVRPTT
Sbjct: 241 IATASGPCGNNREYLFRLENALFEIGHEDDMVIELANEVRNILGKKVRKGKKKQAVRPTT 250

Query: 301 HLPFLSFISGVQVHPLADAAVGTDSYEENVKIGMVKGESHGNSRAKKTSNL 352
           HLPFLSFISGVQVHPLADAAV  DS EEN KIGMVKGESHGNSRAKKTSNL
Sbjct: 301 HLPFLSFISGVQVHPLADAAVAMDSCEENGKIGMVKGESHGNSRAKKTSNL 250

BLAST of Carg03865 vs. NCBI nr
Match: KAG6585408.1 (Proteasome subunit beta type-6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 414.1 bits (1063), Expect = 1.2e-111
Identity = 224/328 (68.29%), Postives = 225/328 (68.60%), Query Frame = 0

Query: 13  WNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGSICQIFVTLPDYS 72
           +N GFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGSIC          
Sbjct: 447 FNHGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGSIC---------- 506

Query: 73  TLSLYQWGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDSIEPTLTGVLVFT 132
                 WGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDSIEPTLTGVLVFT
Sbjct: 507 ------WGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDSIEPTLTGVLVFT 566

Query: 133 STLDKLLNKYYLGPAPLEEMASLEAKPEMESTKRTISASGGSGLLQMVSEPDTGRCASFF 192
           STLDKLLNKYYLGPAPLEEMAS                                      
Sbjct: 567 STLDKLLNKYYLGPAPLEEMAS-------------------------------------- 626

Query: 193 AVPRRGVDTRRCASKDAGPRRGVDLMVVPHRLKEGKSASEDAGPRRGQIATASGPCGNNR 252
                                                          QIATASGPCGNNR
Sbjct: 627 -----------------------------------------------QIATASGPCGNNR 673

Query: 253 DYLFRLENALFEIGHGDDMVIELANEVRNILGKKVRKGKKKQAVRPTTHLPFLSFISGVQ 312
           DYLFRLENALFEIGHGDDMVIELANEVRNIL KKVRKGKKKQAVRPTTHLPFLSFISGVQ
Sbjct: 687 DYLFRLENALFEIGHGDDMVIELANEVRNILVKKVRKGKKKQAVRPTTHLPFLSFISGVQ 673

Query: 313 VHPLADAAVGTDSYEENVKIGMVKGESH 341
           VHPLADAAVGTDSYEENVKIGMVKGESH
Sbjct: 747 VHPLADAAVGTDSYEENVKIGMVKGESH 673

BLAST of Carg03865 vs. ExPASy Swiss-Prot
Match: Q8GY54 (Gamma-glutamylcyclotransferase 2-1 OS=Arabidopsis thaliana OX=3702 GN=GGCT2;1 PE=1 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.6e-64
Identity = 130/283 (45.94%), Postives = 156/283 (55.12%), Query Frame = 0

Query: 1   MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS 60
           MVLWVFGYGSL+WNPGFDFDE++IG+I+DY+RVFDLAC DHRGTP++PART TLE   G+
Sbjct: 1   MVLWVFGYGSLIWNPGFDFDEKLIGYIKDYKRVFDLACIDHRGTPEHPARTCTLEQSTGA 60

Query: 61  ICQIFVTLPDYSTLSLYQWGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDS 120
           IC                WG AYC+ GG E+E+  +E LERRECEYD KT+V+FY + D+
Sbjct: 61  IC----------------WGAAYCVRGGPEKEKLAMEYLERRECEYDSKTLVEFYTENDT 120

Query: 121 IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMASLEAKPEMESTKRTISASGGSGLLQMV 180
             P +TGV+VFTST DK+ NKYYLGPAPLEEMA                           
Sbjct: 121 STPIVTGVIVFTSTPDKVSNKYYLGPAPLEEMAR-------------------------- 180

Query: 181 SEPDTGRCASFFAVPRRGVDTRRCASKDAGPRRGVDLMVVPHRLKEGKSASEDAGPRRGQ 240
                                                                      Q
Sbjct: 181 -----------------------------------------------------------Q 182

Query: 241 IATASGPCGNNRDYLFRLENALFEIGHGDDMVIELANEVRNIL 284
           IATASGPCGNNR+YLF+LE A+F+I H ++ VIELANEVR  L
Sbjct: 241 IATASGPCGNNREYLFKLEKAMFDIEHEEEYVIELANEVRKQL 182

BLAST of Carg03865 vs. ExPASy Swiss-Prot
Match: Q84MC1 (Gamma-glutamylcyclotransferase 2-2 OS=Arabidopsis thaliana OX=3702 GN=GGCT2;2 PE=1 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 3.4e-64
Identity = 136/301 (45.18%), Postives = 163/301 (54.15%), Query Frame = 0

Query: 1   MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS 60
           MV+WVFGYGSLVWNPGF +DE+V+GFI+ Y+RVFDLAC DHRGTP++PART TLE  E +
Sbjct: 1   MVMWVFGYGSLVWNPGFHYDEKVLGFIKGYKRVFDLACIDHRGTPEHPARTCTLEKAEEA 60

Query: 61  ICQIFVTLPDYSTLSLYQWGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDS 120
           IC                WG A+C+ GG E+ER  +E LERRECEYD KT VDFYK+ D 
Sbjct: 61  IC----------------WGTAFCVRGGPEKERLAMEYLERRECEYDLKTSVDFYKEDDP 120

Query: 121 IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMASLEAKPEMESTKRTISASGGSGLLQMV 180
           ++P +TGV+VFTST DK+ NKYYLGPAPLE+MA                           
Sbjct: 121 LKPAVTGVIVFTSTPDKVSNKYYLGPAPLEDMAR-------------------------- 180

Query: 181 SEPDTGRCASFFAVPRRGVDTRRCASKDAGPRRGVDLMVVPHRLKEGKSASEDAGPRRGQ 240
                                                                      Q
Sbjct: 181 -----------------------------------------------------------Q 200

Query: 241 IATASGPCGNNRDYLFRLENALFEIGHGDDMVIELANEVRNILG----KKVRKGKKKQAV 298
           IATA+GPCGNNRDYLF LE A+ +IGH +D VIELANEVR +L     KKV   K+ +A 
Sbjct: 241 IATANGPCGNNRDYLFLLEKAMHDIGHEEDYVIELANEVRKVLAESSTKKVTPVKESRAS 200

BLAST of Carg03865 vs. ExPASy Swiss-Prot
Match: Q84QC1 (Gamma-glutamylcyclotransferase 2-3 OS=Arabidopsis thaliana OX=3702 GN=GGCT2;3 PE=1 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 3.7e-34
Identity = 95/285 (33.33%), Postives = 123/285 (43.16%), Query Frame = 0

Query: 1   MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS 60
           M +WVFGYGSL+W  GF FDE + GFI+ YRRVF     DHRGTP  P RT+TLE     
Sbjct: 1   MAMWVFGYGSLIWKTGFPFDESLPGFIKGYRRVFHQGSTDHRGTPDFPGRTVTLEAAHEE 60

Query: 61  ICQIFVTLPDYSTLSLYQWGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDS 120
           +C                 GVAY I    ++  A++  LE RE +YD K  +DF+ D ++
Sbjct: 61  VC----------------CGVAYKITKEEDKRDALLH-LEVREKQYDQKEYLDFFTDSNA 120

Query: 121 IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMASLEAKPEMESTKRTISASGGSGLLQMV 180
            EP + GV+V+ ++ DK  N  YLGPAPLE++A                           
Sbjct: 121 SEPAVAGVMVYIASPDKKSNNNYLGPAPLEDIAK-------------------------- 180

Query: 181 SEPDTGRCASFFAVPRRGVDTRRCASKDAGPRRGVDLMVVPHRLKEGKSASEDAGPRRGQ 240
                                                                      Q
Sbjct: 181 -----------------------------------------------------------Q 183

Query: 241 IATASGPCGNNRDYLFRLENALFEIGHGDDMVIELANEVRNILGK 286
           I  A GP G NRDYLF LE AL ++G  D  V +LAN+VR+IL +
Sbjct: 241 IVKAKGPSGPNRDYLFNLEEALAQLGFKDKHVTDLANQVRHILSE 183

BLAST of Carg03865 vs. ExPASy Swiss-Prot
Match: Q5ZI66 (Putative glutathione-specific gamma-glutamylcyclotransferase 2 OS=Gallus gallus OX=9031 GN=chac2 PE=2 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 2.8e-18
Identity = 62/152 (40.79%), Postives = 77/152 (50.66%), Query Frame = 0

Query: 3   LWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTL-EPKEGSI 62
           +WVFGYGSL+W   F + E+++G IR Y R F     DHRG P  P R +TL E  EG +
Sbjct: 1   MWVFGYGSLIWKVDFPYQEKMVGRIRGYSRRFWQGSTDHRGVPGKPGRVVTLVEDPEGCV 60

Query: 63  CQIFVTLPDYSTLSLYQWGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDSI 122
                            WGVAY +  G E E  V   L+ RE      T V FY    SI
Sbjct: 61  -----------------WGVAYRLPAGQECE--VKAYLDFREKGGYRTTTVVFYPKDSSI 120

Query: 123 EPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMA 154
           +P    VL++  T D   N  YLGPAPL+E+A
Sbjct: 121 KP--FDVLLYIGTRD---NPNYLGPAPLQEIA 128

BLAST of Carg03865 vs. ExPASy Swiss-Prot
Match: Q4KMJ1 (Putative glutathione-specific gamma-glutamylcyclotransferase 2 OS=Danio rerio OX=7955 GN=chac2 PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 8.2e-18
Identity = 60/166 (36.14%), Postives = 82/166 (49.40%), Query Frame = 0

Query: 3   LWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTL-EPKEGSI 62
           +WVFGYGSL+W   F ++++ +GF++ + R F     DHRG P  P R +TL E  EG +
Sbjct: 1   MWVFGYGSLIWKVDFPYEDKRVGFVKGFSRRFWQGSTDHRGVPGKPGRVVTLIEDPEGCV 60

Query: 63  CQIFVTLPDYSTLSLYQWGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDSI 122
                            WGVAY +  G E+E  V E L+ RE    G   V F+  KD  
Sbjct: 61  -----------------WGVAYKLPSGQEQE--VKEYLDYREKGGYGVITVTFH-PKDEQ 120

Query: 123 EPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMASLEAKPEMESTKRT 168
           +  +   L++  + D   N  YLGPAPLE +A    K    S K T
Sbjct: 121 QQPMQDTLLYIGSCD---NPNYLGPAPLETIAQQIVKSVGPSGKNT 143

BLAST of Carg03865 vs. ExPASy TrEMBL
Match: A0A6J1GIS4 (Gamma-glutamylcyclotransferase OS=Cucurbita moschata OX=3662 GN=LOC111454251 PE=3 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 1.5e-126
Identity = 247/353 (69.97%), Postives = 248/353 (70.25%), Query Frame = 0

Query: 1   MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS 60
           MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS
Sbjct: 1   MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS 60

Query: 61  ICQIFVTLPDYSTLSLYQWGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDS 120
           IC                WGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDS
Sbjct: 61  IC----------------WGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDS 120

Query: 121 IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMASLEAKPEMESTKRTISASGGSGLLQMV 180
           IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMAS                          
Sbjct: 121 IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMAS-------------------------- 180

Query: 181 SEPDTGRCASFFAVPRRGVDTRRCASKDAGPRRGVDLMVVPHRLKEGKSASEDAGPRRGQ 240
                                                                      Q
Sbjct: 181 -----------------------------------------------------------Q 240

Query: 241 IATASGPCGNNRDYLFRLENALFEIGHGDDMVIELANEVRNILGKKVRKGKKKQAVRPTT 300
           IATASGPCGNNRDYLFRLENALFEIGH DDMVIELANEVRNILGKKVRKGKKKQ VRPTT
Sbjct: 241 IATASGPCGNNRDYLFRLENALFEIGHEDDMVIELANEVRNILGKKVRKGKKKQPVRPTT 252

Query: 301 HLPFLSFISGVQVHPLADAAVGTDSYEENVKIGMVKGESHGNSRAKKTSNLVD 354
           HLPFLSFISGVQVHPLADAAV TDSYEEN KIGM+KGESHGNSRAKKTSNLVD
Sbjct: 301 HLPFLSFISGVQVHPLADAAVATDSYEENGKIGMMKGESHGNSRAKKTSNLVD 252

BLAST of Carg03865 vs. ExPASy TrEMBL
Match: A0A6J1KQK0 (Gamma-glutamylcyclotransferase OS=Cucurbita maxima OX=3661 GN=LOC111496304 PE=3 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 1.3e-119
Identity = 237/351 (67.52%), Postives = 240/351 (68.38%), Query Frame = 0

Query: 1   MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS 60
           MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS
Sbjct: 1   MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS 60

Query: 61  ICQIFVTLPDYSTLSLYQWGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDS 120
           IC                WGVAYCIHGGSERERA +E LERRECEYDGKTIV+FYKDKDS
Sbjct: 61  IC----------------WGVAYCIHGGSERERAAMECLERRECEYDGKTIVNFYKDKDS 120

Query: 121 IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMASLEAKPEMESTKRTISASGGSGLLQMV 180
           IEPTLTGVLVFTSTLDKLLNKYYLG A LEEMA                           
Sbjct: 121 IEPTLTGVLVFTSTLDKLLNKYYLGHATLEEMAC-------------------------- 180

Query: 181 SEPDTGRCASFFAVPRRGVDTRRCASKDAGPRRGVDLMVVPHRLKEGKSASEDAGPRRGQ 240
                                                                      Q
Sbjct: 181 -----------------------------------------------------------Q 240

Query: 241 IATASGPCGNNRDYLFRLENALFEIGHGDDMVIELANEVRNILGKKVRKGKKKQAVRPTT 300
           IATASGPCGNNR+YLFRLENALFEIGH DDMVIELANEVRNILGKKVRKGKKKQAVRPTT
Sbjct: 241 IATASGPCGNNREYLFRLENALFEIGHEDDMVIELANEVRNILGKKVRKGKKKQAVRPTT 250

Query: 301 HLPFLSFISGVQVHPLADAAVGTDSYEENVKIGMVKGESHGNSRAKKTSNL 352
           HLPFLSFISGVQVHPLADAAV  DS EEN KIGMVKGESHGNSRAKKTSNL
Sbjct: 301 HLPFLSFISGVQVHPLADAAVAMDSCEENGKIGMVKGESHGNSRAKKTSNL 250

BLAST of Carg03865 vs. ExPASy TrEMBL
Match: A0A0A0LLN4 (Gamma-glutamylcyclotransferase OS=Cucumis sativus OX=3659 GN=Csa_2G377370 PE=3 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 8.9e-92
Identity = 189/330 (57.27%), Postives = 201/330 (60.91%), Query Frame = 0

Query: 1   MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS 60
           MVLWVFGYGSLVWNPGFDFDE+VIGFIRDYRRVFDLAC DHRGTP+NPARTLTLEPKEGS
Sbjct: 100 MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPENPARTLTLEPKEGS 159

Query: 61  ICQIFVTLPDYSTLSLYQWGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDS 120
           IC                WG A+CI GG ERER  +E LE+RECEYD KTIVDFYKD+DS
Sbjct: 160 IC----------------WGAAFCIRGGPEREREAMEYLEKRECEYDQKTIVDFYKDEDS 219

Query: 121 IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMASLEAKPEMESTKRTISASGGSGLLQMV 180
           +EPTLTGVLVFTST DK+LNKYYLGPAPLE+MAS                          
Sbjct: 220 MEPTLTGVLVFTSTFDKVLNKYYLGPAPLEQMAS-------------------------- 279

Query: 181 SEPDTGRCASFFAVPRRGVDTRRCASKDAGPRRGVDLMVVPHRLKEGKSASEDAGPRRGQ 240
                                                                      Q
Sbjct: 280 -----------------------------------------------------------Q 328

Query: 241 IATASGPCGNNRDYLFRLENALFEIGHGDDMVIELANEVRNILGK-----KVRKGKKKQA 300
           IATASGPCGNNRDYLFRLE ALF+IGH DDMVIELANEVRNILGK      V K KKK  
Sbjct: 340 IATASGPCGNNRDYLFRLEKALFDIGHEDDMVIELANEVRNILGKVGKVGMVLKDKKKPI 328

Query: 301 VRPTTHLPFLSFISGVQVHPLADAAVGTDS 326
              TTHLPF+SFISGVQVHP+ DA V  DS
Sbjct: 400 GPTTTHLPFMSFISGVQVHPMVDATVAMDS 328

BLAST of Carg03865 vs. ExPASy TrEMBL
Match: A0A1S3BBC1 (Gamma-glutamylcyclotransferase OS=Cucumis melo OX=3656 GN=LOC103488216 PE=3 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 2.6e-91
Identity = 189/331 (57.10%), Postives = 206/331 (62.24%), Query Frame = 0

Query: 1   MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS 60
           MVLWVFGYGSLVWNPGFDFDE+VIGFIRDYRRVFDLAC DHRGTP+NPARTLTLEPKEGS
Sbjct: 1   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPENPARTLTLEPKEGS 60

Query: 61  ICQIFVTLPDYSTLSLYQWGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDS 120
           IC                WG A+CI GG ERER  +E LE+RECEYD KTIV+FYKD+DS
Sbjct: 61  IC----------------WGAAFCIRGGPEREREAMEYLEKRECEYDQKTIVEFYKDEDS 120

Query: 121 IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMASLEAKPEMESTKRTISASGGSGLLQMV 180
           +EPTLTGVLVFTST DK+LNKYYLGPAPLE+MAS                          
Sbjct: 121 MEPTLTGVLVFTSTFDKVLNKYYLGPAPLEQMAS-------------------------- 180

Query: 181 SEPDTGRCASFFAVPRRGVDTRRCASKDAGPRRGVDLMVVPHRLKEGKSASEDAGPRRGQ 240
                                                                      Q
Sbjct: 181 -----------------------------------------------------------Q 229

Query: 241 IATASGPCGNNRDYLFRLENALFEIGHGDDMVIELANEVRNILGKKVRKG----KKKQAV 300
           IATASGPCGNNRDYLFRLE ALF+IGH DDMVIELANEVRNILG KV KG    +KK+ +
Sbjct: 241 IATASGPCGNNRDYLFRLEKALFDIGHEDDMVIELANEVRNILG-KVGKGVVLKEKKKPI 229

Query: 301 RP--TTHLPFLSFISGVQVHPLADAAVGTDS 326
            P  TTHLPF+SFISG+QVHP+ADA V  DS
Sbjct: 301 GPTTTTHLPFMSFISGIQVHPMADATVAMDS 229

BLAST of Carg03865 vs. ExPASy TrEMBL
Match: A0A6J1HCZ2 (Gamma-glutamylcyclotransferase OS=Cucurbita moschata OX=3662 GN=LOC111462945 PE=3 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 3.4e-91
Identity = 190/325 (58.46%), Postives = 201/325 (61.85%), Query Frame = 0

Query: 1   MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS 60
           MVLWVFGYGSLVWNPGFDFDE+VIGFIRDYRRVFDLAC DHRGTP+NPARTLTLEP EGS
Sbjct: 1   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPENPARTLTLEPTEGS 60

Query: 61  ICQIFVTLPDYSTLSLYQWGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDS 120
           IC                WG AYCI G  ERER  +E LERRECEYD KTIVDFYKD+DS
Sbjct: 61  IC----------------WGAAYCIRGDPEREREAMEYLERRECEYDQKTIVDFYKDEDS 120

Query: 121 IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMASLEAKPEMESTKRTISASGGSGLLQMV 180
           +EPTLTGVLVFTST DKLLNKYYLGPAPLE+MAS                          
Sbjct: 121 MEPTLTGVLVFTSTPDKLLNKYYLGPAPLEQMAS-------------------------- 180

Query: 181 SEPDTGRCASFFAVPRRGVDTRRCASKDAGPRRGVDLMVVPHRLKEGKSASEDAGPRRGQ 240
                                                                      Q
Sbjct: 181 -----------------------------------------------------------Q 220

Query: 241 IATASGPCGNNRDYLFRLENALFEIGHGDDMVIELANEVRNILGKKVRKGKKKQAVRPTT 300
           IATASGPCGNNRDYLFRLE ALF+IGH +D VIELANEVRN+LGK    GKKK  V PTT
Sbjct: 241 IATASGPCGNNRDYLFRLEKALFDIGHEEDTVIELANEVRNVLGK---VGKKK-PVGPTT 220

Query: 301 HLPFLSFISGVQVHPLADAAVGTDS 326
           HLPF+SFISGVQVHPLA+AAV  DS
Sbjct: 301 HLPFMSFISGVQVHPLAEAAVAIDS 220

BLAST of Carg03865 vs. TAIR 10
Match: AT5G26220.1 (ChaC-like family protein )

HSP 1 Score: 247.3 bits (630), Expect = 1.9e-65
Identity = 130/283 (45.94%), Postives = 156/283 (55.12%), Query Frame = 0

Query: 1   MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS 60
           MVLWVFGYGSL+WNPGFDFDE++IG+I+DY+RVFDLAC DHRGTP++PART TLE   G+
Sbjct: 1   MVLWVFGYGSLIWNPGFDFDEKLIGYIKDYKRVFDLACIDHRGTPEHPARTCTLEQSTGA 60

Query: 61  ICQIFVTLPDYSTLSLYQWGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDS 120
           IC                WG AYC+ GG E+E+  +E LERRECEYD KT+V+FY + D+
Sbjct: 61  IC----------------WGAAYCVRGGPEKEKLAMEYLERRECEYDSKTLVEFYTENDT 120

Query: 121 IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMASLEAKPEMESTKRTISASGGSGLLQMV 180
             P +TGV+VFTST DK+ NKYYLGPAPLEEMA                           
Sbjct: 121 STPIVTGVIVFTSTPDKVSNKYYLGPAPLEEMAR-------------------------- 180

Query: 181 SEPDTGRCASFFAVPRRGVDTRRCASKDAGPRRGVDLMVVPHRLKEGKSASEDAGPRRGQ 240
                                                                      Q
Sbjct: 181 -----------------------------------------------------------Q 182

Query: 241 IATASGPCGNNRDYLFRLENALFEIGHGDDMVIELANEVRNIL 284
           IATASGPCGNNR+YLF+LE A+F+I H ++ VIELANEVR  L
Sbjct: 241 IATASGPCGNNREYLFKLEKAMFDIEHEEEYVIELANEVRKQL 182

BLAST of Carg03865 vs. TAIR 10
Match: AT4G31290.1 (ChaC-like family protein )

HSP 1 Score: 246.9 bits (629), Expect = 2.4e-65
Identity = 136/301 (45.18%), Postives = 163/301 (54.15%), Query Frame = 0

Query: 1   MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS 60
           MV+WVFGYGSLVWNPGF +DE+V+GFI+ Y+RVFDLAC DHRGTP++PART TLE  E +
Sbjct: 1   MVMWVFGYGSLVWNPGFHYDEKVLGFIKGYKRVFDLACIDHRGTPEHPARTCTLEKAEEA 60

Query: 61  ICQIFVTLPDYSTLSLYQWGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDS 120
           IC                WG A+C+ GG E+ER  +E LERRECEYD KT VDFYK+ D 
Sbjct: 61  IC----------------WGTAFCVRGGPEKERLAMEYLERRECEYDLKTSVDFYKEDDP 120

Query: 121 IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMASLEAKPEMESTKRTISASGGSGLLQMV 180
           ++P +TGV+VFTST DK+ NKYYLGPAPLE+MA                           
Sbjct: 121 LKPAVTGVIVFTSTPDKVSNKYYLGPAPLEDMAR-------------------------- 180

Query: 181 SEPDTGRCASFFAVPRRGVDTRRCASKDAGPRRGVDLMVVPHRLKEGKSASEDAGPRRGQ 240
                                                                      Q
Sbjct: 181 -----------------------------------------------------------Q 200

Query: 241 IATASGPCGNNRDYLFRLENALFEIGHGDDMVIELANEVRNILG----KKVRKGKKKQAV 298
           IATA+GPCGNNRDYLF LE A+ +IGH +D VIELANEVR +L     KKV   K+ +A 
Sbjct: 241 IATANGPCGNNRDYLFLLEKAMHDIGHEEDYVIELANEVRKVLAESSTKKVTPVKESRAS 200

BLAST of Carg03865 vs. TAIR 10
Match: AT1G44790.1 (ChaC-like family protein )

HSP 1 Score: 147.1 bits (370), Expect = 2.6e-35
Identity = 95/285 (33.33%), Postives = 123/285 (43.16%), Query Frame = 0

Query: 1   MVLWVFGYGSLVWNPGFDFDERVIGFIRDYRRVFDLACFDHRGTPQNPARTLTLEPKEGS 60
           M +WVFGYGSL+W  GF FDE + GFI+ YRRVF     DHRGTP  P RT+TLE     
Sbjct: 1   MAMWVFGYGSLIWKTGFPFDESLPGFIKGYRRVFHQGSTDHRGTPDFPGRTVTLEAAHEE 60

Query: 61  ICQIFVTLPDYSTLSLYQWGVAYCIHGGSERERAVVESLERRECEYDGKTIVDFYKDKDS 120
           +C                 GVAY I    ++  A++  LE RE +YD K  +DF+ D ++
Sbjct: 61  VC----------------CGVAYKITKEEDKRDALLH-LEVREKQYDQKEYLDFFTDSNA 120

Query: 121 IEPTLTGVLVFTSTLDKLLNKYYLGPAPLEEMASLEAKPEMESTKRTISASGGSGLLQMV 180
            EP + GV+V+ ++ DK  N  YLGPAPLE++A                           
Sbjct: 121 SEPAVAGVMVYIASPDKKSNNNYLGPAPLEDIAK-------------------------- 180

Query: 181 SEPDTGRCASFFAVPRRGVDTRRCASKDAGPRRGVDLMVVPHRLKEGKSASEDAGPRRGQ 240
                                                                      Q
Sbjct: 181 -----------------------------------------------------------Q 183

Query: 241 IATASGPCGNNRDYLFRLENALFEIGHGDDMVIELANEVRNILGK 286
           I  A GP G NRDYLF LE AL ++G  D  V +LAN+VR+IL +
Sbjct: 241 IVKAKGPSGPNRDYLFNLEEALAQLGFKDKHVTDLANQVRHILSE 183

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7020326.11.0e-203100.00Gamma-glutamylcyclotransferase 2-1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022951435.13.0e-12669.97gamma-glutamylcyclotransferase 2-1-like [Cucurbita moschata][more]
XP_023537463.14.4e-12569.80gamma-glutamylcyclotransferase 2-1-like [Cucurbita pepo subsp. pepo][more]
XP_023002479.12.7e-11967.52gamma-glutamylcyclotransferase 2-1-like [Cucurbita maxima][more]
KAG6585408.11.2e-11168.29Proteasome subunit beta type-6, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q8GY542.6e-6445.94Gamma-glutamylcyclotransferase 2-1 OS=Arabidopsis thaliana OX=3702 GN=GGCT2;1 PE... [more]
Q84MC13.4e-6445.18Gamma-glutamylcyclotransferase 2-2 OS=Arabidopsis thaliana OX=3702 GN=GGCT2;2 PE... [more]
Q84QC13.7e-3433.33Gamma-glutamylcyclotransferase 2-3 OS=Arabidopsis thaliana OX=3702 GN=GGCT2;3 PE... [more]
Q5ZI662.8e-1840.79Putative glutathione-specific gamma-glutamylcyclotransferase 2 OS=Gallus gallus ... [more]
Q4KMJ18.2e-1836.14Putative glutathione-specific gamma-glutamylcyclotransferase 2 OS=Danio rerio OX... [more]
Match NameE-valueIdentityDescription
A0A6J1GIS41.5e-12669.97Gamma-glutamylcyclotransferase OS=Cucurbita moschata OX=3662 GN=LOC111454251 PE=... [more]
A0A6J1KQK01.3e-11967.52Gamma-glutamylcyclotransferase OS=Cucurbita maxima OX=3661 GN=LOC111496304 PE=3 ... [more]
A0A0A0LLN48.9e-9257.27Gamma-glutamylcyclotransferase OS=Cucumis sativus OX=3659 GN=Csa_2G377370 PE=3 S... [more]
A0A1S3BBC12.6e-9157.10Gamma-glutamylcyclotransferase OS=Cucumis melo OX=3656 GN=LOC103488216 PE=3 SV=1[more]
A0A6J1HCZ23.4e-9158.46Gamma-glutamylcyclotransferase OS=Cucurbita moschata OX=3662 GN=LOC111462945 PE=... [more]
Match NameE-valueIdentityDescription
AT5G26220.11.9e-6545.94ChaC-like family protein [more]
AT4G31290.12.4e-6545.18ChaC-like family protein [more]
AT1G44790.12.6e-3533.33ChaC-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006840Glutathione-specific gamma-glutamylcyclotransferasePFAMPF04752ChaCcoord: 3..155
e-value: 4.2E-29
score: 101.9
coord: 239..281
e-value: 5.4E-5
score: 23.3
IPR006840Glutathione-specific gamma-glutamylcyclotransferasePANTHERPTHR12192CATION TRANSPORT PROTEIN CHAC-RELATEDcoord: 1..154
IPR006840Glutathione-specific gamma-glutamylcyclotransferasePANTHERPTHR12192CATION TRANSPORT PROTEIN CHAC-RELATEDcoord: 240..324
NoneNo IPR availablePANTHERPTHR12192:SF23GAMMA-GLUTAMYLCYCLOTRANSFERASE 2-2coord: 240..324
NoneNo IPR availablePANTHERPTHR12192:SF23GAMMA-GLUTAMYLCYCLOTRANSFERASE 2-2coord: 1..154
IPR013024Gamma-glutamyl cyclotransferase-likeCDDcd06661GGCT_likecoord: 5..107
e-value: 2.35754E-4
score: 38.0947

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg03865-RACarg03865-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006751 glutathione catabolic process
molecular_function GO:0003839 gamma-glutamylcyclotransferase activity