Carg03803 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg03803
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	40S ribosomal protein S27
Location	Carg_Chr08: 105562 .. 106203 (+)
RNA-Seq Expression	Carg03803
Synteny	Carg03803

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTTATAACGTTCATTGCACCGCCCTGATTCTTTCGCGAACTCTCGTTGGGATTCTAGGGTTCGCGATCCAGACAGTTCCTTACTGATCAACATGGTAAACTTCCTGCTACATTCGATTATGCCTTTTTATCTCTACTGAATTTGGATCCAACAATTTTCACTTTCTTTCACGCTATTTTCAGGTTCTTCAAAACGATATTGATTTGCTCAATCCTCCGGCCGAACTTGAGAAGAGAAAACATAAGCTCAAGCGTCTCGTACAGTCGCCCAACTCATTCTTCATGGTTTGCTCTTTCGTTTAAATTCTTTTTGCTCTCTTTGATCTAATCTTTTCTCCGATAATGGTGATCGAGCTGTCTTGTGATTTATATATGCAGGATGTGAAGTGCCAGGGTTGCTTTAACATGTAAGTTTTCATCTTTTCTCATGTATTATGGTTATGTTACCTTTCTTTTTTCTGCAAGTTGTTGAAGTTGGGTTCTTAAGTGAGTAATGGCACATAACTCTTTGAATGAATAACAGAACCACTGTATTCAGTCATTCACAGACAGTCGTGGTATGTGGGAATTGCCAGTCGGTCTTGTGCCAGCCAACTGGAGGGCGCGCTAGGCTAACAGAAGGCTGCTCCTTCAGAAGAAAG

mRNA sequence

TTTTTATAACGTTCATTGCACCGCCCTGATTCTTTCGCGAACTCTCGTTGGGATTCTAGGGTTCGCGATCCAGACAGTTCCTTACTGATCAACATGGTTCTTCAAAACGATATTGATTTGCTCAATCCTCCGGCCGAACTTGAGAAGAGAAAACATAAGCTCAAGCGTCTCGTACAGTCGCCCAACTCATTCTTCATGGTTTGCTCTTTCGTTTAAATTCTTTTTGCTCTCTTTGATCTAATCTTTTCTCCGATAATGGTGATCGAGCTGTCTTGTGATTTATATATGCAGGATGTGAAGTGCCAGGGTTGCTTTAACATAACCACTGTATTCAGTCATTCACAGACAGTCGTGGTATGTGGGAATTGCCAGTCGGTCTTGTGCCAGCCAACTGGAGGGCGCGCTAGGCTAACAGAAGGCTGCTCCTTCAGAAGAAAG

Coding sequence (CDS)

ATGGTGATCGAGCTGTCTTGTGATTTATATATGCAGGATGTGAAGTGCCAGGGTTGCTTTAACATAACCACTGTATTCAGTCATTCACAGACAGTCGTGGTATGTGGGAATTGCCAGTCGGTCTTGTGCCAGCCAACTGGAGGGCGCGCTAGGCTAACAGAAGGCTGCTCCTTCAGAAGAAAG

Protein sequence

MVIELSCDLYMQDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK

Homology

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BLAST of Carg03803 vs. NCBI nr
Match: KAG7025236.1 (40S ribosomal protein S27-2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 132.1 bits (331), Expect = 1.6e-27
Identity = 61/61 (100.00%), Postives = 61/61 (100.00%), Query Frame = 0


Query: 1  MVIELSCDLYMQDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRR 60
          MVIELSCDLYMQDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRR
Sbjct: 1  MVIELSCDLYMQDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRR 60

Query: 61 K 62
          K
Sbjct: 61 K 61

[Close]

BLAST of Carg03803 vs. NCBI nr
Match: TQD77936.1 (hypothetical protein C1H46_036469 [Malus baccata])

HSP 1 Score: 111.7 bits (278), Expect = 2.3e-21
Identity = 50/61 (81.97%), Postives = 54/61 (88.52%), Query Frame = 0


Query: 1  MVIELSCDLYMQDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRR 60
          M++   C    QDVKCQGCF+ITTVFSHSQTVVVCGNCQ+VLCQPTGGRARLTEGCSFRR
Sbjct: 35 MIVNFDCFKCAQDVKCQGCFSITTVFSHSQTVVVCGNCQTVLCQPTGGRARLTEGCSFRR 94

Query: 61 K 62
          K
Sbjct: 95 K 95

[Close]

BLAST of Carg03803 vs. NCBI nr
Match: XP_006348533.1 (PREDICTED: 40S ribosomal protein S27-2 isoform X1 [Solanum tuberosum] >XP_006348534.1 PREDICTED: 40S ribosomal protein S27-2 isoform X2 [Solanum tuberosum] >ABB87121.1 ribosomal protein S27-like protein-like [Solanum tuberosum])

HSP 1 Score: 110.5 bits (275), Expect = 5.0e-21
Identity = 50/56 (89.29%), Postives = 52/56 (92.86%), Query Frame = 0


Query: 6  SCDLYMQDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK 62
          S D +  DVKCQGCFNITTVFSHSQTVVVCGNCQ+VLCQPTGGRARLTEGCSFRRK
Sbjct: 29 SPDSFFMDVKCQGCFNITTVFSHSQTVVVCGNCQTVLCQPTGGRARLTEGCSFRRK 84

[Close]

BLAST of Carg03803 vs. NCBI nr
Match: WP_214429920.1 (hypothetical protein, partial [Escherichia coli])

HSP 1 Score: 110.5 bits (275), Expect = 5.0e-21
Identity = 50/56 (89.29%), Postives = 52/56 (92.86%), Query Frame = 0


Query: 6   SCDLYMQDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK 62
           S D +  DVKCQGCFNITTVFSHSQTVVVCGNCQ+VLCQPTGGRARLTEGCSFRRK
Sbjct: 52  SPDSFFMDVKCQGCFNITTVFSHSQTVVVCGNCQTVLCQPTGGRARLTEGCSFRRK 107

[Close]

BLAST of Carg03803 vs. NCBI nr
Match: XP_022960233.1 (40S ribosomal protein S27-2-like [Cucurbita moschata] >XP_023004242.1 40S ribosomal protein S27-2-like [Cucurbita maxima] >XP_023513990.1 40S ribosomal protein S27-2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 109.8 bits (273), Expect = 8.6e-21
Identity = 49/52 (94.23%), Postives = 50/52 (96.15%), Query Frame = 0


Query: 10 YMQDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK 62
          +  DVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK
Sbjct: 33 FFMDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK 84

[Close]

BLAST of Carg03803 vs. ExPASy Swiss-Prot
Match: Q9M2F1 (40S ribosomal protein S27-2 OS=Arabidopsis thaliana OX=3702 GN=RPS27B PE=2 SV=2)

HSP 1 Score: 105.9 bits (263), Expect = 1.6e-22
Identity = 45/52 (86.54%), Postives = 50/52 (96.15%), Query Frame = 0


Query: 10 YMQDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK 62
          +  DVKCQGCFNITTVFSHSQTVVVCGNCQ++LCQPTGG+A+LTEGCSFRRK
Sbjct: 33 FFMDVKCQGCFNITTVFSHSQTVVVCGNCQTILCQPTGGKAKLTEGCSFRRK 84

[Close]

BLAST of Carg03803 vs. ExPASy Swiss-Prot
Match: Q8L953 (40S ribosomal protein S27-3 OS=Arabidopsis thaliana OX=3702 GN=RPS27D PE=2 SV=2)

HSP 1 Score: 104.0 bits (258), Expect = 6.2e-22
Identity = 45/52 (86.54%), Postives = 49/52 (94.23%), Query Frame = 0


Query: 10 YMQDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK 62
          +  DVKCQGCFNITTVFSHSQTVVVCGNCQ+VLCQPTGG+ARL EGCSFR+K
Sbjct: 33 FFMDVKCQGCFNITTVFSHSQTVVVCGNCQTVLCQPTGGKARLQEGCSFRKK 84

[Close]

BLAST of Carg03803 vs. ExPASy Swiss-Prot
Match: Q96564 (40S ribosomal protein S27 OS=Hordeum vulgare OX=4513 GN=RPS27 PE=3 SV=2)

HSP 1 Score: 102.1 bits (253), Expect = 2.3e-21
Identity = 45/52 (86.54%), Postives = 48/52 (92.31%), Query Frame = 0


Query: 10 YMQDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK 62
          +  DVKCQGCFNITTVFSHSQTVVVC  CQ+VLCQPTGG+ARLTEGCSFRRK
Sbjct: 33 FFMDVKCQGCFNITTVFSHSQTVVVCPGCQTVLCQPTGGKARLTEGCSFRRK 84

[Close]

BLAST of Carg03803 vs. ExPASy Swiss-Prot
Match: O64650 (40S ribosomal protein S27-1 OS=Arabidopsis thaliana OX=3702 GN=RPS27A PE=3 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 6.8e-21
Identity = 42/52 (80.77%), Postives = 49/52 (94.23%), Query Frame = 0


Query: 10 YMQDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK 62
          +  DVKCQGCFNITTVFSHSQTVV+CGNCQ++LC PTGG+A+LTEGCSFR+K
Sbjct: 33 FFMDVKCQGCFNITTVFSHSQTVVMCGNCQTLLCTPTGGKAKLTEGCSFRKK 84

[Close]

BLAST of Carg03803 vs. ExPASy Swiss-Prot
Match: O74330 (40S ribosomal protein S27 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rps27 PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 3.4e-20
Identity = 42/52 (80.77%), Postives = 47/52 (90.38%), Query Frame = 0


Query: 10 YMQDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK 62
          +  DVKC GCFNITTVFSH+QTVV+CG+C SVLCQPTGG+ARL EGCSFRRK
Sbjct: 31 FFMDVKCPGCFNITTVFSHAQTVVICGSCASVLCQPTGGKARLMEGCSFRRK 82

[Close]

BLAST of Carg03803 vs. ExPASy TrEMBL
Match: A0A540KUR0 (40S ribosomal protein S27 OS=Malus baccata OX=106549 GN=C1H46_036469 PE=3 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-21
Identity = 50/61 (81.97%), Postives = 54/61 (88.52%), Query Frame = 0


Query: 1  MVIELSCDLYMQDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRR 60
          M++   C    QDVKCQGCF+ITTVFSHSQTVVVCGNCQ+VLCQPTGGRARLTEGCSFRR
Sbjct: 35 MIVNFDCFKCAQDVKCQGCFSITTVFSHSQTVVVCGNCQTVLCQPTGGRARLTEGCSFRR 94

Query: 61 K 62
          K
Sbjct: 95 K 95

[Close]

BLAST of Carg03803 vs. ExPASy TrEMBL
Match: Q2VCJ1 (40S ribosomal protein S27 OS=Solanum tuberosum OX=4113 GN=102600252 PE=2 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 2.4e-21
Identity = 50/56 (89.29%), Postives = 52/56 (92.86%), Query Frame = 0


Query: 6  SCDLYMQDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK 62
          S D +  DVKCQGCFNITTVFSHSQTVVVCGNCQ+VLCQPTGGRARLTEGCSFRRK
Sbjct: 29 SPDSFFMDVKCQGCFNITTVFSHSQTVVVCGNCQTVLCQPTGGRARLTEGCSFRRK 84

[Close]

BLAST of Carg03803 vs. ExPASy TrEMBL
Match: A0A2H5QKC8 (40S ribosomal protein S27 OS=Citrus unshiu OX=55188 GN=CUMW_211090 PE=3 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 4.2e-21
Identity = 49/52 (94.23%), Postives = 50/52 (96.15%), Query Frame = 0


Query: 10 YMQDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK 62
          +  DVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK
Sbjct: 33 FFMDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK 84

[Close]

BLAST of Carg03803 vs. ExPASy TrEMBL
Match: V4W2J8 (40S ribosomal protein S27 OS=Citrus clementina OX=85681 GN=CICLE_v10017332mg PE=3 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 4.2e-21
Identity = 49/52 (94.23%), Postives = 50/52 (96.15%), Query Frame = 0


Query: 10 YMQDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK 62
          +  DVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK
Sbjct: 33 FFMDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK 84

[Close]

BLAST of Carg03803 vs. ExPASy TrEMBL
Match: A0A6J1KPW3 (40S ribosomal protein S27 OS=Cucurbita maxima OX=3661 GN=LOC111497636 PE=3 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 4.2e-21
Identity = 49/52 (94.23%), Postives = 50/52 (96.15%), Query Frame = 0


Query: 10 YMQDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK 62
          +  DVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK
Sbjct: 33 FFMDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK 84

[Close]

BLAST of Carg03803 vs. TAIR 10
Match: AT3G61110.1 (ribosomal protein S27 )

HSP 1 Score: 105.9 bits (263), Expect = 1.2e-23
Identity = 45/52 (86.54%), Postives = 50/52 (96.15%), Query Frame = 0


Query: 10 YMQDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK 62
          +  DVKCQGCFNITTVFSHSQTVVVCGNCQ++LCQPTGG+A+LTEGCSFRRK
Sbjct: 33 FFMDVKCQGCFNITTVFSHSQTVVVCGNCQTILCQPTGGKAKLTEGCSFRRK 84

[Close]

BLAST of Carg03803 vs. TAIR 10
Match: AT5G47930.1 (Zinc-binding ribosomal protein family protein )

HSP 1 Score: 104.0 bits (258), Expect = 4.4e-23
Identity = 45/52 (86.54%), Postives = 49/52 (94.23%), Query Frame = 0


Query: 10 YMQDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK 62
          +  DVKCQGCFNITTVFSHSQTVVVCGNCQ+VLCQPTGG+ARL EGCSFR+K
Sbjct: 33 FFMDVKCQGCFNITTVFSHSQTVVVCGNCQTVLCQPTGGKARLQEGCSFRKK 84

[Close]

BLAST of Carg03803 vs. TAIR 10
Match: AT2G45710.1 (Zinc-binding ribosomal protein family protein )

HSP 1 Score: 100.5 bits (249), Expect = 4.9e-22
Identity = 42/52 (80.77%), Postives = 49/52 (94.23%), Query Frame = 0


Query: 10 YMQDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRRK 62
          +  DVKCQGCFNITTVFSHSQTVV+CGNCQ++LC PTGG+A+LTEGCSFR+K
Sbjct: 33 FFMDVKCQGCFNITTVFSHSQTVVMCGNCQTLLCTPTGGKAKLTEGCSFRKK 84

[Close]

BLAST of Carg03803 vs. TAIR 10
Match: AT3G61111.1 (Zinc-binding ribosomal protein family protein )

HSP 1 Score: 87.4 bits (215), Expect = 4.2e-18
Identity = 37/51 (72.55%), Postives = 43/51 (84.31%), Query Frame = 0


Query: 10 YMQDVKCQGCFNITTVFSHSQTVVVCGNCQSVLCQPTGGRARLTEGCSFRR 61
          Y   V+C  C NITT+FSHSQTVVVCG CQ+VLCQPTGG+A+LT GCSFR+
Sbjct: 33 YFMLVRCSDCINITTIFSHSQTVVVCGKCQNVLCQPTGGKAKLTVGCSFRK 83

The following BLAST results are available for this feature:

BLAST of Carg03803 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7025236.1	1.6e-27	100.00	40S ribosomal protein S27-2, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]
TQD77936.1	2.3e-21	81.97	hypothetical protein C1H46_036469 [Malus baccata]	[more]
XP_006348533.1	5.0e-21	89.29	PREDICTED: 40S ribosomal protein S27-2 isoform X1 [Solanum tuberosum] >XP_006348...	[more]
WP_214429920.1	5.0e-21	89.29	hypothetical protein, partial [Escherichia coli]	[more]
XP_022960233.1	8.6e-21	94.23	40S ribosomal protein S27-2-like [Cucurbita moschata] >XP_023004242.1 40S riboso...	[more]

BLAST of Carg03803 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2F1	1.6e-22	86.54	40S ribosomal protein S27-2 OS=Arabidopsis thaliana OX=3702 GN=RPS27B PE=2 SV=2	[more]
Q8L953	6.2e-22	86.54	40S ribosomal protein S27-3 OS=Arabidopsis thaliana OX=3702 GN=RPS27D PE=2 SV=2	[more]
Q96564	2.3e-21	86.54	40S ribosomal protein S27 OS=Hordeum vulgare OX=4513 GN=RPS27 PE=3 SV=2	[more]
O64650	6.8e-21	80.77	40S ribosomal protein S27-1 OS=Arabidopsis thaliana OX=3702 GN=RPS27A PE=3 SV=1	[more]
O74330	3.4e-20	80.77	40S ribosomal protein S27 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843)...	[more]

BLAST of Carg03803 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A540KUR0	1.1e-21	81.97	40S ribosomal protein S27 OS=Malus baccata OX=106549 GN=C1H46_036469 PE=3 SV=1	[more]
Q2VCJ1	2.4e-21	89.29	40S ribosomal protein S27 OS=Solanum tuberosum OX=4113 GN=102600252 PE=2 SV=1	[more]
A0A2H5QKC8	4.2e-21	94.23	40S ribosomal protein S27 OS=Citrus unshiu OX=55188 GN=CUMW_211090 PE=3 SV=1	[more]
V4W2J8	4.2e-21	94.23	40S ribosomal protein S27 OS=Citrus clementina OX=85681 GN=CICLE_v10017332mg PE=...	[more]
A0A6J1KPW3	4.2e-21	94.23	40S ribosomal protein S27 OS=Cucurbita maxima OX=3661 GN=LOC111497636 PE=3 SV=1	[more]

BLAST of Carg03803 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G61110.1	1.2e-23	86.54	ribosomal protein S27	[more]
AT5G47930.1	4.4e-23	86.54	Zinc-binding ribosomal protein family protein	[more]
AT2G45710.1	4.9e-22	80.77	Zinc-binding ribosomal protein family protein	[more]
AT3G61111.1	4.2e-18	72.55	Zinc-binding ribosomal protein family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000592	Ribosomal protein S27	PFAM	PF01667	Ribosomal_S27e	coord: 13..61 e-value: 3.2E-24 score: 84.4
IPR000592	Ribosomal protein S27	PANTHER	PTHR11594	40S RIBOSOMAL PROTEIN S27	coord: 10..61
IPR000592	Ribosomal protein S27	PROSITE	PS01168	RIBOSOMAL_S27E	coord: 15..47
IPR023407	Ribosomal protein S27, zinc-binding domain superfamily	GENE3D	2.20.25.100		coord: 5..61 e-value: 1.3E-28 score: 100.7
None	No IPR available	PANTHER	PTHR11594:SF7	40S RIBOSOMAL PROTEIN S27-RELATED	coord: 10..61
IPR011332	Zinc-binding ribosomal protein	SUPERFAMILY	57829	Zn-binding ribosomal proteins	coord: 10..61

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg03803-RA	Carg03803-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000028	ribosomal small subunit assembly
biological_process	GO:0006412	translation
cellular_component	GO:0022627	cytosolic small ribosomal subunit
cellular_component	GO:0043231	intracellular membrane-bounded organelle
cellular_component	GO:0005840	ribosome
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0003735	structural constituent of ribosome