Carg03799 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg03799
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionEukaryotic peptide chain release factor subunit 1-1
LocationCarg_Chr08: 120463 .. 121773 (+)
RNA-Seq ExpressionCarg03799
SyntenyCarg03799
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGACGGTCATGAAACTGATAAGAACATTGAAATATGGAAAATTAAGAAGTTGATTAAAGCACTTGAAGCTGCTAGAGGAAATGGGACTAGTATGATATCTCTTATCATGCCTCCGCGTGATCAAATATCTCGTGTTACTAAGATGCTTGGTGATGAATTTGGAACTGCTTCGAACATTAAGAGCAGGGTGAATCGCCAATCTGTTTTGGGTGCTATCACATCTGCTCAGCAGAGACTCAAGCTGTATAGCAAAGTTCCTCCGAATGGGCTTGTGCTTTATACTGGGACTATCGTGACTGAAGATGGAAAAGAAAAGAAAGTCACTATTGATTTTGAACCTTTCAGACCTATAAATGCATCTCTGTATCTCTGTGACAATAAGTTCCATACGGAAGCCCTGAATGAACTTTTAGAATCTGATGACAAGTTTGGCTTCATTGTCATGGATGGTAATGGAACACTCTTTGGTACATTGAGTGGTAACACACGTGAGGTTCTTCACAAATTTAGTGTTGACCTTCCTAAGAAACATGGAAGAGGAGGTCAATCAGCACTTAGGTTTGCCCGTCTTCGGATGGAGAAGCGGCACAACTATGTCAGGAAAACAGCAGAGCTTGCAACCCAGTTCTTTATTAATCCTGCCACTAGTCAACCTAATGTTGCAGGATTGATACTGGCTGGATCAGCCGACTTCAAAACAGAGCTCAGTCAATCAGACATGTTTGATCCTCGTCTTCAAACAAAAATACTTAATGTAGTTGATGTGTCTTATGGAGGAGAAAATGGATTTAATCAGGCCATTGAACTGTCCTCCGAGATCTTGTCTAATGTAAAATTTATACAGGAGAAGCGCTTGATTGGAAAATACTTTGAAGAGATTAGCCAGGACACGGGGAAATATGTTTTTGGTGTTGATGACACACTGAAAGCTCTGGAGATGGGCGCTGTTGAGATACTCATTGTTTGGGAAAATTTGGATATCAACAGGTATGTTTTAAAGAATGCTTCCACTGGTGAGATTGTTATAAAGCACTTGAATAAGGAACAGGAGGCCAATCAGAGCAACTTCCGTGACTCCGTCACCTCTGCTGAGTTGGAGGTTCAAGAAAAGGTGGCCTTGCTGGAATGGTTTGCAAACGAGTACAAACAGTTTGGTTGCACACTTGAGTTCGTCACCAACAAATCACAGGAAGGATCACAGTTCTGCAGGGGTTTTGGTGGTATTGGAGGAATTCTTCGTTACCAGCTTGACATGAGATCGTTTGATGAGCTATCTGATGGGGAAGAGTACGGTGATTCTGAATAG

mRNA sequence

ATGGCTGACGGTCATGAAACTGATAAGAACATTGAAATATGGAAAATTAAGAAGTTGATTAAAGCACTTGAAGCTGCTAGAGGAAATGGGACTAGTATGATATCTCTTATCATGCCTCCGCGTGATCAAATATCTCGTGTTACTAAGATGCTTGGTGATGAATTTGGAACTGCTTCGAACATTAAGAGCAGGGTGAATCGCCAATCTGTTTTGGGTGCTATCACATCTGCTCAGCAGAGACTCAAGCTGTATAGCAAAGTTCCTCCGAATGGGCTTGTGCTTTATACTGGGACTATCGTGACTGAAGATGGAAAAGAAAAGAAAGTCACTATTGATTTTGAACCTTTCAGACCTATAAATGCATCTCTGTATCTCTGTGACAATAAGTTCCATACGGAAGCCCTGAATGAACTTTTAGAATCTGATGACAAGTTTGGCTTCATTGTCATGGATGGTAATGGAACACTCTTTGGTACATTGAGTGGTAACACACGTGAGGTTCTTCACAAATTTAGTGTTGACCTTCCTAAGAAACATGGAAGAGGAGGTCAATCAGCACTTAGGTTTGCCCGTCTTCGGATGGAGAAGCGGCACAACTATGTCAGGAAAACAGCAGAGCTTGCAACCCAGTTCTTTATTAATCCTGCCACTAGTCAACCTAATGTTGCAGGATTGATACTGGCTGGATCAGCCGACTTCAAAACAGAGCTCAGTCAATCAGACATGTTTGATCCTCGTCTTCAAACAAAAATACTTAATGTAGTTGATGTGTCTTATGGAGGAGAAAATGGATTTAATCAGGCCATTGAACTGTCCTCCGAGATCTTGTCTAATGTAAAATTTATACAGGAGAAGCGCTTGATTGGAAAATACTTTGAAGAGATTAGCCAGGACACGGGGAAATATGTTTTTGGTGTTGATGACACACTGAAAGCTCTGGAGATGGGCGCTGTTGAGATACTCATTGTTTGGGAAAATTTGGATATCAACAGGTATGTTTTAAAGAATGCTTCCACTGGTGAGATTGTTATAAAGCACTTGAATAAGGAACAGGAGGCCAATCAGAGCAACTTCCGTGACTCCGTCACCTCTGCTGAGTTGGAGGTTCAAGAAAAGGTGGCCTTGCTGGAATGGTTTGCAAACGAGTACAAACAGTTTGGTTGCACACTTGAGTTCGTCACCAACAAATCACAGGAAGGATCACAGTTCTGCAGGGGTTTTGGTGGTATTGGAGGAATTCTTCGTTACCAGCTTGACATGAGATCGTTTGATGAGCTATCTGATGGGGAAGAGTACGGTGATTCTGAATAG

Coding sequence (CDS)

ATGGCTGACGGTCATGAAACTGATAAGAACATTGAAATATGGAAAATTAAGAAGTTGATTAAAGCACTTGAAGCTGCTAGAGGAAATGGGACTAGTATGATATCTCTTATCATGCCTCCGCGTGATCAAATATCTCGTGTTACTAAGATGCTTGGTGATGAATTTGGAACTGCTTCGAACATTAAGAGCAGGGTGAATCGCCAATCTGTTTTGGGTGCTATCACATCTGCTCAGCAGAGACTCAAGCTGTATAGCAAAGTTCCTCCGAATGGGCTTGTGCTTTATACTGGGACTATCGTGACTGAAGATGGAAAAGAAAAGAAAGTCACTATTGATTTTGAACCTTTCAGACCTATAAATGCATCTCTGTATCTCTGTGACAATAAGTTCCATACGGAAGCCCTGAATGAACTTTTAGAATCTGATGACAAGTTTGGCTTCATTGTCATGGATGGTAATGGAACACTCTTTGGTACATTGAGTGGTAACACACGTGAGGTTCTTCACAAATTTAGTGTTGACCTTCCTAAGAAACATGGAAGAGGAGGTCAATCAGCACTTAGGTTTGCCCGTCTTCGGATGGAGAAGCGGCACAACTATGTCAGGAAAACAGCAGAGCTTGCAACCCAGTTCTTTATTAATCCTGCCACTAGTCAACCTAATGTTGCAGGATTGATACTGGCTGGATCAGCCGACTTCAAAACAGAGCTCAGTCAATCAGACATGTTTGATCCTCGTCTTCAAACAAAAATACTTAATGTAGTTGATGTGTCTTATGGAGGAGAAAATGGATTTAATCAGGCCATTGAACTGTCCTCCGAGATCTTGTCTAATGTAAAATTTATACAGGAGAAGCGCTTGATTGGAAAATACTTTGAAGAGATTAGCCAGGACACGGGGAAATATGTTTTTGGTGTTGATGACACACTGAAAGCTCTGGAGATGGGCGCTGTTGAGATACTCATTGTTTGGGAAAATTTGGATATCAACAGGTATGTTTTAAAGAATGCTTCCACTGGTGAGATTGTTATAAAGCACTTGAATAAGGAACAGGAGGCCAATCAGAGCAACTTCCGTGACTCCGTCACCTCTGCTGAGTTGGAGGTTCAAGAAAAGGTGGCCTTGCTGGAATGGTTTGCAAACGAGTACAAACAGTTTGGTTGCACACTTGAGTTCGTCACCAACAAATCACAGGAAGGATCACAGTTCTGCAGGGGTTTTGGTGGTATTGGAGGAATTCTTCGTTACCAGCTTGACATGAGATCGTTTGATGAGCTATCTGATGGGGAAGAGTACGGTGATTCTGAATAG

Protein sequence

MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASNIKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPINASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHGRGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQSDMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTGKYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRDSVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDMRSFDELSDGEEYGDSE
Homology
BLAST of Carg03799 vs. NCBI nr
Match: XP_022960455.1 (eukaryotic peptide chain release factor subunit 1-3-like [Cucurbita moschata] >XP_022960456.1 eukaryotic peptide chain release factor subunit 1-3-like [Cucurbita moschata] >XP_023514284.1 eukaryotic peptide chain release factor subunit 1-3-like [Cucurbita pepo subsp. pepo] >XP_023514285.1 eukaryotic peptide chain release factor subunit 1-3-like [Cucurbita pepo subsp. pepo] >KAG7025240.1 Eukaryotic peptide chain release factor subunit 1-3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 866.3 bits (2237), Expect = 1.1e-247
Identity = 436/436 (100.00%), Postives = 436/436 (100.00%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN
Sbjct: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360
           KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD
Sbjct: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360

Query: 361 SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420
           SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM
Sbjct: 361 SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420

Query: 421 RSFDELSDGEEYGDSE 437
           RSFDELSDGEEYGDSE
Sbjct: 421 RSFDELSDGEEYGDSE 436

BLAST of Carg03799 vs. NCBI nr
Match: XP_023004292.1 (eukaryotic peptide chain release factor subunit 1-3-like [Cucurbita maxima] >XP_023004293.1 eukaryotic peptide chain release factor subunit 1-3-like [Cucurbita maxima] >XP_023004294.1 eukaryotic peptide chain release factor subunit 1-3-like [Cucurbita maxima])

HSP 1 Score: 864.8 bits (2233), Expect = 3.3e-247
Identity = 435/436 (99.77%), Postives = 435/436 (99.77%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN
Sbjct: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360
           KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQE NQSNFRD
Sbjct: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQETNQSNFRD 360

Query: 361 SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420
           SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM
Sbjct: 361 SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420

Query: 421 RSFDELSDGEEYGDSE 437
           RSFDELSDGEEYGDSE
Sbjct: 421 RSFDELSDGEEYGDSE 436

BLAST of Carg03799 vs. NCBI nr
Match: XP_008447536.1 (PREDICTED: eukaryotic peptide chain release factor subunit 1-3 [Cucumis melo] >XP_008447544.1 PREDICTED: eukaryotic peptide chain release factor subunit 1-3 [Cucumis melo] >KAA0039870.1 eukaryotic peptide chain release factor subunit 1-3 [Cucumis melo var. makuwa] >KAA0047345.1 eukaryotic peptide chain release factor subunit 1-3 [Cucumis melo var. makuwa] >TYK24631.1 eukaryotic peptide chain release factor subunit 1-3 [Cucumis melo var. makuwa])

HSP 1 Score: 852.4 bits (2201), Expect = 1.7e-243
Identity = 428/436 (98.17%), Postives = 432/436 (99.08%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MAD HETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN
Sbjct: 1   MADAHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLY+KVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRLQ KILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360
           KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD
Sbjct: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360

Query: 361 SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420
           S TSAELEVQEK+ALLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLD+
Sbjct: 361 SATSAELEVQEKMALLEWFANEYKKFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420

Query: 421 RSFDELSDGEEYGDSE 437
           RSFDELSDGEEY DSE
Sbjct: 421 RSFDELSDGEEYDDSE 436

BLAST of Carg03799 vs. NCBI nr
Match: KAG7014612.1 (Eukaryotic peptide chain release factor subunit 1-3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 852.4 bits (2201), Expect = 1.7e-243
Identity = 425/436 (97.48%), Postives = 433/436 (99.31%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN
Sbjct: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLY+KVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQF+INPATSQPNVAGLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVAGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRLQ KILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360
           KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKN STGE++IKHLNKEQEANQSNFRD
Sbjct: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNVSTGEVIIKHLNKEQEANQSNFRD 360

Query: 361 SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420
            VT+AELEVQEK+ALLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLD+
Sbjct: 361 PVTAAELEVQEKMALLEWFANEYKKFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420

Query: 421 RSFDELSDGEEYGDSE 437
           RSFDELSDGEEYGDSE
Sbjct: 421 RSFDELSDGEEYGDSE 436

BLAST of Carg03799 vs. NCBI nr
Match: KAG6576095.1 (Eukaryotic peptide chain release factor subunit 1-3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 852.4 bits (2201), Expect = 1.7e-243
Identity = 425/436 (97.48%), Postives = 433/436 (99.31%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN
Sbjct: 106 MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 165

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLY+KVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN
Sbjct: 166 IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 225

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 226 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 285

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQF+INPATSQPNVAGLILAGSADFKTELSQS
Sbjct: 286 RGGQSALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVAGLILAGSADFKTELSQS 345

Query: 241 DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRLQ KILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG
Sbjct: 346 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 405

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360
           KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKN STGE++IKHLNKEQEANQSNFRD
Sbjct: 406 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNVSTGEVIIKHLNKEQEANQSNFRD 465

Query: 361 SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420
            VT+AELEVQEK+ALLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLD+
Sbjct: 466 PVTAAELEVQEKMALLEWFANEYKKFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 525

Query: 421 RSFDELSDGEEYGDSE 437
           RSFDELSDGEEYGDSE
Sbjct: 526 RSFDELSDGEEYGDSE 541

BLAST of Carg03799 vs. ExPASy Swiss-Prot
Match: P35614 (Eukaryotic peptide chain release factor subunit 1-3 OS=Arabidopsis thaliana OX=3702 GN=ERF1-3 PE=1 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 1.6e-228
Identity = 394/436 (90.37%), Postives = 418/436 (95.87%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MAD  E+DKNIEIWKIKKLIK LE ARGNGTSMISLIMPPRDQ++RVTKML DE+GTASN
Sbjct: 1   MAD-QESDKNIEIWKIKKLIKGLETARGNGTSMISLIMPPRDQVARVTKMLADEYGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVL AITSAQQRLKLY+KVPPNGLVLYTGTIVT+DGKEKKVTIDFEPF+PIN
Sbjct: 61  IKSRVNRQSVLSAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKVTIDFEPFKPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTE LNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKF+VDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEPLNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFTVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQF+INPATSQPNV+GLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVSGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           ++FDPRLQ KILNVVDVSYGGENGFNQAIELS+EILSNVKFIQEK+LIGKYFEEISQDTG
Sbjct: 241 ELFDPRLQAKILNVVDVSYGGENGFNQAIELSAEILSNVKFIQEKKLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360
           KYVFGV+DTLKALEMGAVE LIVWENLDINRY LKN +TGEIVIKHL K+QE NQSNF D
Sbjct: 301 KYVFGVEDTLKALEMGAVETLIVWENLDINRYELKNNTTGEIVIKHLGKDQENNQSNFHD 360

Query: 361 SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420
           + T+AELEVQEK+ LLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGG+LRYQLDM
Sbjct: 361 AETNAELEVQEKMPLLEWFANEYKRFGCTLEFVTNKSQEGSQFCRGFGGIGGLLRYQLDM 420

Query: 421 RSFDELSDGEEYGDSE 437
           R+FDELSDGE Y DS+
Sbjct: 421 RTFDELSDGEVYEDSD 435

BLAST of Carg03799 vs. ExPASy Swiss-Prot
Match: D2K759 (Eukaryotic peptide chain release factor subunit 1-2 OS=Brassica oleracea var. botrytis OX=3715 GN=ERF1-2 PE=1 SV=1)

HSP 1 Score: 785.0 bits (2026), Expect = 4.3e-226
Identity = 389/436 (89.22%), Postives = 415/436 (95.18%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MAD  E D NIEIWKIKKLIK LE+ARGNGTSMISLIMPPRDQ+SRVTKMLGDE+GTASN
Sbjct: 1   MAD-QEADTNIEIWKIKKLIKGLESARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVL AITSAQQRLKLY+KVP NGLVLYTGTIV +DGKEKKVT DFEPFRPIN
Sbjct: 61  IKSRVNRQSVLSAITSAQQRLKLYNKVPTNGLVLYTGTIVNDDGKEKKVTFDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHT ALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKF+VDLPKKHG
Sbjct: 121 ASLYLCDNKFHTGALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFTVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQF+INPATSQPNVAGLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVAGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           ++FDPRLQ KILNVVDVSYGGENGFNQAIELS+EILSNVKFIQEK+LIGKYFEEISQDTG
Sbjct: 241 ELFDPRLQAKILNVVDVSYGGENGFNQAIELSAEILSNVKFIQEKKLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360
           KYVFGV+DTLKALEMGA+E LIVWENLDINRY LKN+++GEIV+KH  K+QE +QSNF D
Sbjct: 301 KYVFGVEDTLKALEMGAIETLIVWENLDINRYELKNSTSGEIVVKHFGKDQETDQSNFHD 360

Query: 361 SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420
           + T+AELEVQEK+ LLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGG+LRYQLDM
Sbjct: 361 AETNAELEVQEKMPLLEWFANEYKRFGCTLEFVTNKSQEGSQFCRGFGGIGGMLRYQLDM 420

Query: 421 RSFDELSDGEEYGDSE 437
           R+FDELSDGE Y DS+
Sbjct: 421 RTFDELSDGEVYEDSD 435

BLAST of Carg03799 vs. ExPASy Swiss-Prot
Match: Q9LPV8 (Eukaryotic peptide chain release factor subunit 1-2 OS=Arabidopsis thaliana OX=3702 GN=ERF1-2 PE=1 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 4.8e-225
Identity = 385/431 (89.33%), Postives = 412/431 (95.59%), Query Frame = 0

Query: 6   ETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASNIKSRV 65
           E D NIEIWKIKKLIK LE+ARGNGTSMISLIMPPRDQ+SRVTKMLGDE+GTASNIKSRV
Sbjct: 4   EADTNIEIWKIKKLIKGLESARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASNIKSRV 63

Query: 66  NRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPINASLYL 125
           NRQSVL AITSAQQRLKLY+KVP NGLVLYTGTIV +DGKEKKVT DFEPFRPINASLYL
Sbjct: 64  NRQSVLSAITSAQQRLKLYNKVPTNGLVLYTGTIVNDDGKEKKVTFDFEPFRPINASLYL 123

Query: 126 CDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHGRGGQS 185
           CDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKF+VDLPKKHGRGGQS
Sbjct: 124 CDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFTVDLPKKHGRGGQS 183

Query: 186 ALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQSDMFDP 245
           ALRFARLRMEKRHNYVRKTAELATQF+INPATSQPNV+GLILAGSADFKTELSQS++FDP
Sbjct: 184 ALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVSGLILAGSADFKTELSQSELFDP 243

Query: 246 RLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTGKYVFG 305
           RLQ KILNVVDVSYGGENGFNQAIELS+EILSNVKFIQEK+LIGKYFEEISQDTGKYVFG
Sbjct: 244 RLQAKILNVVDVSYGGENGFNQAIELSAEILSNVKFIQEKKLIGKYFEEISQDTGKYVFG 303

Query: 306 VDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRDSVTSA 365
           V+DTLKALEMGA+E LIVWENLDINRY LKN++TGE+V+KH  K+QE++ SNF DS T+A
Sbjct: 304 VEDTLKALEMGAIETLIVWENLDINRYELKNSTTGEMVVKHFGKDQESDTSNFHDSETNA 363

Query: 366 ELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDMRSFDE 425
           ELEVQEK+ LLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGG+LRYQLDMR+FDE
Sbjct: 364 ELEVQEKMPLLEWFANEYKRFGCTLEFVTNKSQEGSQFCRGFGGIGGMLRYQLDMRTFDE 423

Query: 426 LSDGEEYGDSE 437
           LSD E Y DS+
Sbjct: 424 LSDTEVYEDSD 434

BLAST of Carg03799 vs. ExPASy Swiss-Prot
Match: D2K760 (Eukaryotic peptide chain release factor subunit 1-3 OS=Brassica oleracea var. botrytis OX=3715 GN=ERF1-3 PE=2 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 1.4e-224
Identity = 387/436 (88.76%), Postives = 415/436 (95.18%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MAD  E+DK+IEIWK  KLIK LE+ARGNGTSMISLIMPPRDQ++RVTKML DE+GTASN
Sbjct: 1   MAD-QESDKSIEIWKSFKLIKGLESARGNGTSMISLIMPPRDQVARVTKMLADEYGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVL AITSAQQRLKLY+KVPPNGLVLYTGTIVT+DGKEKKVTIDFEPF+PIN
Sbjct: 61  IKSRVNRQSVLSAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKVTIDFEPFKPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTE LNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKF+VDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEPLNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFTVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQF+INPATSQPNV+GLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVSGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           ++FDPRLQ KILNVVDVSYGGENGFNQAIELS+EILSNVKFIQEK+LIGKYFEEISQDTG
Sbjct: 241 ELFDPRLQAKILNVVDVSYGGENGFNQAIELSAEILSNVKFIQEKKLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360
           KYVFGVDDTLKAL+MGAVE LIVWENLDINRY LKN +TGE VIKHL KEQE +QSNF D
Sbjct: 301 KYVFGVDDTLKALDMGAVETLIVWENLDINRYELKNGATGETVIKHLGKEQENDQSNFHD 360

Query: 361 SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420
           + ++AELE+ EK+ LLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGG+LRYQLDM
Sbjct: 361 AESNAELEIVEKMPLLEWFANEYKRFGCTLEFVTNKSQEGSQFCRGFGGIGGLLRYQLDM 420

Query: 421 RSFDELSDGEEYGDSE 437
           R+FDELSDGE Y DS+
Sbjct: 421 RTFDELSDGEVYEDSD 435

BLAST of Carg03799 vs. ExPASy Swiss-Prot
Match: Q39097 (Eukaryotic peptide chain release factor subunit 1-1 OS=Arabidopsis thaliana OX=3702 GN=ERF1-1 PE=1 SV=2)

HSP 1 Score: 769.6 bits (1986), Expect = 1.9e-221
Identity = 381/434 (87.79%), Postives = 408/434 (94.01%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           M D ++ DKNIEIWKIKKLIK+LEAARGNGTSMISLIMPPRDQ+SRVTKMLGDE+GTASN
Sbjct: 1   MGDKNDDDKNIEIWKIKKLIKSLEAARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLY++VPPNGLVLYTGTIV EDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYNRVPPNGLVLYTGTIVNEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQ++INPATSQPNV+GLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQYYINPATSQPNVSGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRL  KILNVVDVSYGGENGFNQAIELS+EIL+NVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLAAKILNVVDVSYGGENGFNQAIELSAEILANVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360
           KYVFGV+DTL ALE GA+E LIVWENLDINRYV+KN++TGE VIKHLNKEQEAN  NF+ 
Sbjct: 301 KYVFGVEDTLNALESGAIETLIVWENLDINRYVMKNSATGETVIKHLNKEQEANTENFKV 360

Query: 361 SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420
           + +   L+V+EK++LLEW ANEY++FGC LEFVTNKSQEGSQFCRGFGGIGGILRYQLDM
Sbjct: 361 ADSDLALDVEEKLSLLEWLANEYRRFGCALEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420

Query: 421 RSFDELSDGEEYGD 435
            +FD   DGE   D
Sbjct: 421 TAFDS-EDGEALDD 433

BLAST of Carg03799 vs. ExPASy TrEMBL
Match: A0A6J1H946 (eukaryotic peptide chain release factor subunit 1-3-like OS=Cucurbita moschata OX=3662 GN=LOC111461184 PE=3 SV=1)

HSP 1 Score: 866.3 bits (2237), Expect = 5.4e-248
Identity = 436/436 (100.00%), Postives = 436/436 (100.00%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN
Sbjct: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360
           KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD
Sbjct: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360

Query: 361 SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420
           SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM
Sbjct: 361 SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420

Query: 421 RSFDELSDGEEYGDSE 437
           RSFDELSDGEEYGDSE
Sbjct: 421 RSFDELSDGEEYGDSE 436

BLAST of Carg03799 vs. ExPASy TrEMBL
Match: A0A6J1KQ13 (eukaryotic peptide chain release factor subunit 1-3-like OS=Cucurbita maxima OX=3661 GN=LOC111497662 PE=3 SV=1)

HSP 1 Score: 864.8 bits (2233), Expect = 1.6e-247
Identity = 435/436 (99.77%), Postives = 435/436 (99.77%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN
Sbjct: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360
           KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQE NQSNFRD
Sbjct: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQETNQSNFRD 360

Query: 361 SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420
           SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM
Sbjct: 361 SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420

Query: 421 RSFDELSDGEEYGDSE 437
           RSFDELSDGEEYGDSE
Sbjct: 421 RSFDELSDGEEYGDSE 436

BLAST of Carg03799 vs. ExPASy TrEMBL
Match: A0A1S3BHN0 (eukaryotic peptide chain release factor subunit 1-3 OS=Cucumis melo OX=3656 GN=LOC103489959 PE=3 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 8.1e-244
Identity = 428/436 (98.17%), Postives = 432/436 (99.08%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MAD HETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN
Sbjct: 1   MADAHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLY+KVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRLQ KILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360
           KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD
Sbjct: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360

Query: 361 SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420
           S TSAELEVQEK+ALLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLD+
Sbjct: 361 SATSAELEVQEKMALLEWFANEYKKFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420

Query: 421 RSFDELSDGEEYGDSE 437
           RSFDELSDGEEY DSE
Sbjct: 421 RSFDELSDGEEYDDSE 436

BLAST of Carg03799 vs. ExPASy TrEMBL
Match: A0A5A7T9D0 (Eukaryotic peptide chain release factor subunit 1-3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G001780 PE=3 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 8.1e-244
Identity = 428/436 (98.17%), Postives = 432/436 (99.08%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MAD HETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN
Sbjct: 1   MADAHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLY+KVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRLQ KILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360
           KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD
Sbjct: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360

Query: 361 SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420
           S TSAELEVQEK+ALLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLD+
Sbjct: 361 SATSAELEVQEKMALLEWFANEYKKFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420

Query: 421 RSFDELSDGEEYGDSE 437
           RSFDELSDGEEY DSE
Sbjct: 421 RSFDELSDGEEYDDSE 436

BLAST of Carg03799 vs. ExPASy TrEMBL
Match: A0A6J1JV14 (eukaryotic peptide chain release factor subunit 1-3 OS=Cucurbita maxima OX=3661 GN=LOC111488098 PE=3 SV=1)

HSP 1 Score: 851.7 bits (2199), Expect = 1.4e-243
Identity = 424/436 (97.25%), Postives = 433/436 (99.31%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN
Sbjct: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLY+KVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQF+INPATSQPNVAGLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVAGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRLQ KILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360
           KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKN STGE++IKHLNKEQEANQSNFRD
Sbjct: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNVSTGEVIIKHLNKEQEANQSNFRD 360

Query: 361 SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420
            +T+AELEVQEK+ALLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLD+
Sbjct: 361 LITAAELEVQEKMALLEWFANEYKKFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420

Query: 421 RSFDELSDGEEYGDSE 437
           RSFDELSDGEEYGDSE
Sbjct: 421 RSFDELSDGEEYGDSE 436

BLAST of Carg03799 vs. TAIR 10
Match: AT3G26618.1 (eukaryotic release factor 1-3 )

HSP 1 Score: 793.1 bits (2047), Expect = 1.1e-229
Identity = 394/436 (90.37%), Postives = 418/436 (95.87%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MAD  E+DKNIEIWKIKKLIK LE ARGNGTSMISLIMPPRDQ++RVTKML DE+GTASN
Sbjct: 1   MAD-QESDKNIEIWKIKKLIKGLETARGNGTSMISLIMPPRDQVARVTKMLADEYGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVL AITSAQQRLKLY+KVPPNGLVLYTGTIVT+DGKEKKVTIDFEPF+PIN
Sbjct: 61  IKSRVNRQSVLSAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKVTIDFEPFKPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTE LNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKF+VDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEPLNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFTVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQF+INPATSQPNV+GLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVSGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           ++FDPRLQ KILNVVDVSYGGENGFNQAIELS+EILSNVKFIQEK+LIGKYFEEISQDTG
Sbjct: 241 ELFDPRLQAKILNVVDVSYGGENGFNQAIELSAEILSNVKFIQEKKLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360
           KYVFGV+DTLKALEMGAVE LIVWENLDINRY LKN +TGEIVIKHL K+QE NQSNF D
Sbjct: 301 KYVFGVEDTLKALEMGAVETLIVWENLDINRYELKNNTTGEIVIKHLGKDQENNQSNFHD 360

Query: 361 SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420
           + T+AELEVQEK+ LLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGG+LRYQLDM
Sbjct: 361 AETNAELEVQEKMPLLEWFANEYKRFGCTLEFVTNKSQEGSQFCRGFGGIGGLLRYQLDM 420

Query: 421 RSFDELSDGEEYGDSE 437
           R+FDELSDGE Y DS+
Sbjct: 421 RTFDELSDGEVYEDSD 435

BLAST of Carg03799 vs. TAIR 10
Match: AT1G12920.1 (eukaryotic release factor 1-2 )

HSP 1 Score: 781.6 bits (2017), Expect = 3.4e-226
Identity = 385/431 (89.33%), Postives = 412/431 (95.59%), Query Frame = 0

Query: 6   ETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASNIKSRV 65
           E D NIEIWKIKKLIK LE+ARGNGTSMISLIMPPRDQ+SRVTKMLGDE+GTASNIKSRV
Sbjct: 4   EADTNIEIWKIKKLIKGLESARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASNIKSRV 63

Query: 66  NRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPINASLYL 125
           NRQSVL AITSAQQRLKLY+KVP NGLVLYTGTIV +DGKEKKVT DFEPFRPINASLYL
Sbjct: 64  NRQSVLSAITSAQQRLKLYNKVPTNGLVLYTGTIVNDDGKEKKVTFDFEPFRPINASLYL 123

Query: 126 CDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHGRGGQS 185
           CDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKF+VDLPKKHGRGGQS
Sbjct: 124 CDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFTVDLPKKHGRGGQS 183

Query: 186 ALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQSDMFDP 245
           ALRFARLRMEKRHNYVRKTAELATQF+INPATSQPNV+GLILAGSADFKTELSQS++FDP
Sbjct: 184 ALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVSGLILAGSADFKTELSQSELFDP 243

Query: 246 RLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTGKYVFG 305
           RLQ KILNVVDVSYGGENGFNQAIELS+EILSNVKFIQEK+LIGKYFEEISQDTGKYVFG
Sbjct: 244 RLQAKILNVVDVSYGGENGFNQAIELSAEILSNVKFIQEKKLIGKYFEEISQDTGKYVFG 303

Query: 306 VDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRDSVTSA 365
           V+DTLKALEMGA+E LIVWENLDINRY LKN++TGE+V+KH  K+QE++ SNF DS T+A
Sbjct: 304 VEDTLKALEMGAIETLIVWENLDINRYELKNSTTGEMVVKHFGKDQESDTSNFHDSETNA 363

Query: 366 ELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDMRSFDE 425
           ELEVQEK+ LLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGG+LRYQLDMR+FDE
Sbjct: 364 ELEVQEKMPLLEWFANEYKRFGCTLEFVTNKSQEGSQFCRGFGGIGGMLRYQLDMRTFDE 423

Query: 426 LSDGEEYGDSE 437
           LSD E Y DS+
Sbjct: 424 LSDTEVYEDSD 434

BLAST of Carg03799 vs. TAIR 10
Match: AT5G47880.1 (eukaryotic release factor 1-1 )

HSP 1 Score: 769.6 bits (1986), Expect = 1.3e-222
Identity = 381/434 (87.79%), Postives = 408/434 (94.01%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           M D ++ DKNIEIWKIKKLIK+LEAARGNGTSMISLIMPPRDQ+SRVTKMLGDE+GTASN
Sbjct: 1   MGDKNDDDKNIEIWKIKKLIKSLEAARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLY++VPPNGLVLYTGTIV EDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYNRVPPNGLVLYTGTIVNEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQ++INPATSQPNV+GLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQYYINPATSQPNVSGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRL  KILNVVDVSYGGENGFNQAIELS+EIL+NVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLAAKILNVVDVSYGGENGFNQAIELSAEILANVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360
           KYVFGV+DTL ALE GA+E LIVWENLDINRYV+KN++TGE VIKHLNKEQEAN  NF+ 
Sbjct: 301 KYVFGVEDTLNALESGAIETLIVWENLDINRYVMKNSATGETVIKHLNKEQEANTENFKV 360

Query: 361 SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420
           + +   L+V+EK++LLEW ANEY++FGC LEFVTNKSQEGSQFCRGFGGIGGILRYQLDM
Sbjct: 361 ADSDLALDVEEKLSLLEWLANEYRRFGCALEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420

Query: 421 RSFDELSDGEEYGD 435
            +FD   DGE   D
Sbjct: 421 TAFDS-EDGEALDD 433

BLAST of Carg03799 vs. TAIR 10
Match: AT5G47880.2 (eukaryotic release factor 1-1 )

HSP 1 Score: 769.6 bits (1986), Expect = 1.3e-222
Identity = 381/434 (87.79%), Postives = 408/434 (94.01%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           M D ++ DKNIEIWKIKKLIK+LEAARGNGTSMISLIMPPRDQ+SRVTKMLGDE+GTASN
Sbjct: 1   MGDKNDDDKNIEIWKIKKLIKSLEAARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLY++VPPNGLVLYTGTIV EDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYNRVPPNGLVLYTGTIVNEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQ++INPATSQPNV+GLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQYYINPATSQPNVSGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRL  KILNVVDVSYGGENGFNQAIELS+EIL+NVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLAAKILNVVDVSYGGENGFNQAIELSAEILANVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360
           KYVFGV+DTL ALE GA+E LIVWENLDINRYV+KN++TGE VIKHLNKEQEAN  NF+ 
Sbjct: 301 KYVFGVEDTLNALESGAIETLIVWENLDINRYVMKNSATGETVIKHLNKEQEANTENFKV 360

Query: 361 SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420
           + +   L+V+EK++LLEW ANEY++FGC LEFVTNKSQEGSQFCRGFGGIGGILRYQLDM
Sbjct: 361 ADSDLALDVEEKLSLLEWLANEYRRFGCALEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420

Query: 421 RSFDELSDGEEYGD 435
            +FD   DGE   D
Sbjct: 421 TAFDS-EDGEALDD 433

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022960455.11.1e-247100.00eukaryotic peptide chain release factor subunit 1-3-like [Cucurbita moschata] >X... [more]
XP_023004292.13.3e-24799.77eukaryotic peptide chain release factor subunit 1-3-like [Cucurbita maxima] >XP_... [more]
XP_008447536.11.7e-24398.17PREDICTED: eukaryotic peptide chain release factor subunit 1-3 [Cucumis melo] >X... [more]
KAG7014612.11.7e-24397.48Eukaryotic peptide chain release factor subunit 1-3, partial [Cucurbita argyrosp... [more]
KAG6576095.11.7e-24397.48Eukaryotic peptide chain release factor subunit 1-3, partial [Cucurbita argyrosp... [more]
Match NameE-valueIdentityDescription
P356141.6e-22890.37Eukaryotic peptide chain release factor subunit 1-3 OS=Arabidopsis thaliana OX=3... [more]
D2K7594.3e-22689.22Eukaryotic peptide chain release factor subunit 1-2 OS=Brassica oleracea var. bo... [more]
Q9LPV84.8e-22589.33Eukaryotic peptide chain release factor subunit 1-2 OS=Arabidopsis thaliana OX=3... [more]
D2K7601.4e-22488.76Eukaryotic peptide chain release factor subunit 1-3 OS=Brassica oleracea var. bo... [more]
Q390971.9e-22187.79Eukaryotic peptide chain release factor subunit 1-1 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1H9465.4e-248100.00eukaryotic peptide chain release factor subunit 1-3-like OS=Cucurbita moschata O... [more]
A0A6J1KQ131.6e-24799.77eukaryotic peptide chain release factor subunit 1-3-like OS=Cucurbita maxima OX=... [more]
A0A1S3BHN08.1e-24498.17eukaryotic peptide chain release factor subunit 1-3 OS=Cucumis melo OX=3656 GN=L... [more]
A0A5A7T9D08.1e-24498.17Eukaryotic peptide chain release factor subunit 1-3 OS=Cucumis melo var. makuwa ... [more]
A0A6J1JV141.4e-24397.25eukaryotic peptide chain release factor subunit 1-3 OS=Cucurbita maxima OX=3661 ... [more]
Match NameE-valueIdentityDescription
AT3G26618.11.1e-22990.37eukaryotic release factor 1-3 [more]
AT1G12920.13.4e-22689.33eukaryotic release factor 1-2 [more]
AT5G47880.11.3e-22287.79eukaryotic release factor 1-1 [more]
AT5G47880.21.3e-22287.79eukaryotic release factor 1-1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005140eRF1 domain 1/Pelota-likeSMARTSM01194eRF1_1_2coord: 4..140
e-value: 9.4E-47
score: 171.3
IPR005140eRF1 domain 1/Pelota-likePFAMPF03463eRF1_1coord: 17..138
e-value: 4.9E-20
score: 71.9
IPR042226eRF1, domain 2 superfamilyGENE3D3.30.420.60eRF1 domain 2coord: 136..277
e-value: 5.7E-61
score: 206.8
IPR005142eRF1 domain 3PFAMPF03465eRF1_3coord: 281..418
e-value: 5.1E-36
score: 123.6
IPR005141eRF1 domain 2PFAMPF03464eRF1_2coord: 144..277
e-value: 2.8E-42
score: 144.2
IPR004403Peptide chain release factor eRF1/aRF1TIGRFAMTIGR03676TIGR03676coord: 12..417
e-value: 1.6E-127
score: 423.6
IPR004403Peptide chain release factor eRF1/aRF1PANTHERPTHR10113PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1coord: 1..436
IPR024049eRF1, domain 1GENE3D3.30.960.10eRF1 domain 1coord: 2..135
e-value: 1.6E-66
score: 224.2
IPR024049eRF1, domain 1SUPERFAMILY55481N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1coord: 6..140
IPR02906450S ribosomal protein L30e-likeGENE3D3.30.1330.30coord: 279..436
e-value: 1.2E-65
score: 222.2
IPR02906450S ribosomal protein L30e-likeSUPERFAMILY55315L30e-likecoord: 278..420
NoneNo IPR availablePANTHERPTHR10113:SF32EUKARYOTIC PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1-3-LIKEcoord: 1..436
NoneNo IPR availableSUPERFAMILY53137Translational machinery componentscoord: 142..276

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg03799-RACarg03799-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002184 cytoplasmic translational termination
biological_process GO:0040008 regulation of growth
biological_process GO:0006415 translational termination
cellular_component GO:0005829 cytosol
cellular_component GO:0018444 translation release factor complex
molecular_function GO:1990825 sequence-specific mRNA binding
molecular_function GO:0016149 translation release factor activity, codon specific
molecular_function GO:0003747 translation release factor activity