Homology
BLAST of Carg03795 vs. NCBI nr
Match:
KAG7025244.1 (Isoflavone 2'-hydroxylase [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1001.1 bits (2587), Expect = 3.3e-288
Identity = 501/501 (100.00%), Postives = 501/501 (100.00%), Query Frame = 0
Query: 1 MDDSTFLYSVLCIALVVAISYALKQKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQ 60
MDDSTFLYSVLCIALVVAISYALKQKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQ
Sbjct: 1 MDDSTFLYSVLCIALVVAISYALKQKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQ 60
Query: 61 KYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYG 120
KYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYG
Sbjct: 61 KYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYG 120
Query: 121 DHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMVKELTL 180
DHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMVKELTL
Sbjct: 121 DHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMVKELTL 180
Query: 181 NITMRMVAGKRYYGEETDESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQGFMKR 240
NITMRMVAGKRYYGEETDESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQGFMKR
Sbjct: 181 NITMRMVAGKRYYGEETDESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQGFMKR 240
Query: 241 AKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILTM 300
AKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILTM
Sbjct: 241 AKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILTM 300
Query: 301 VLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLGDQLLEESEVPKLSYLRCIVLET 360
VLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLGDQLLEESEVPKLSYLRCIVLET
Sbjct: 301 VLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLGDQLLEESEVPKLSYLRCIVLET 360
Query: 361 LRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEGG 420
LRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEGG
Sbjct: 361 LRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEGG 420
Query: 421 DGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGKGVSM 480
DGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGKGVSM
Sbjct: 421 DGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGKGVSM 480
Query: 481 PKAEPLEALCKPRPIASKLLL 502
PKAEPLEALCKPRPIASKLLL
Sbjct: 481 PKAEPLEALCKPRPIASKLLL 501
BLAST of Carg03795 vs. NCBI nr
Match:
XP_022959856.1 (isoflavone 2'-hydroxylase-like [Cucurbita moschata])
HSP 1 Score: 987.6 bits (2552), Expect = 3.8e-284
Identity = 495/501 (98.80%), Postives = 495/501 (98.80%), Query Frame = 0
Query: 1 MDDSTFLYSVLCIALVVAISYALKQKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQ 60
MDDSTFLYS LCIALVVAISYALK KS KNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQ
Sbjct: 1 MDDSTFLYSALCIALVVAISYALKPKSHKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQ 60
Query: 61 KYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYG 120
KYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYG
Sbjct: 61 KYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYG 120
Query: 121 DHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMVKELTL 180
DHWRKLRRVSALEILSANRLNTNSAIRRDEVRRL NKLSQ SRDDFARVEFKSMVKELTL
Sbjct: 121 DHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLANKLSQTSRDDFARVEFKSMVKELTL 180
Query: 181 NITMRMVAGKRYYGEETDESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQGFMKR 240
NITMRMVAGKRYYGEETDESSEA TFRAIVDELFEFMLAQYPADFLPILKYIDIQGFMKR
Sbjct: 181 NITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDIQGFMKR 240
Query: 241 AKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILTM 300
AKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILTM
Sbjct: 241 AKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILTM 300
Query: 301 VLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLGDQLLEESEVPKLSYLRCIVLET 360
VLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLGDQLLEESEVPKLSYLRCIVLET
Sbjct: 301 VLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLGDQLLEESEVPKLSYLRCIVLET 360
Query: 361 LRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEGG 420
LRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEGG
Sbjct: 361 LRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEGG 420
Query: 421 DGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGKGVSM 480
DGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGKGVSM
Sbjct: 421 DGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGKGVSM 480
Query: 481 PKAEPLEALCKPRPIASKLLL 502
PKAEPLEALCKPRPIASKLLL
Sbjct: 481 PKAEPLEALCKPRPIASKLLL 501
BLAST of Carg03795 vs. NCBI nr
Match:
XP_023005007.1 (isoflavone 2'-hydroxylase-like [Cucurbita maxima])
HSP 1 Score: 984.9 bits (2545), Expect = 2.5e-283
Identity = 493/501 (98.40%), Postives = 497/501 (99.20%), Query Frame = 0
Query: 1 MDDSTFLYSVLCIALVVAISYALKQKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQ 60
MDDSTFLYSVLCIALVVAISYALK KSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQ
Sbjct: 1 MDDSTFLYSVLCIALVVAISYALKPKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQ 60
Query: 61 KYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYG 120
KYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYG
Sbjct: 61 KYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYG 120
Query: 121 DHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMVKELTL 180
DHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQ SRDDFARVEFKSMVKELTL
Sbjct: 121 DHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVKELTL 180
Query: 181 NITMRMVAGKRYYGEETDESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQGFMKR 240
NITMRMVAGKRYYGEET+ESSEARTFRAIV+ELFEFMLAQYPADFLPILKYID QGFMKR
Sbjct: 181 NITMRMVAGKRYYGEETNESSEARTFRAIVNELFEFMLAQYPADFLPILKYIDFQGFMKR 240
Query: 241 AKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILTM 300
AKKLIKRVDAFWQGIVDEHRTNQ+HNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILTM
Sbjct: 241 AKKLIKRVDAFWQGIVDEHRTNQVHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILTM 300
Query: 301 VLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLGDQLLEESEVPKLSYLRCIVLET 360
VLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLGDQLLEESEVPKLSYLRCIVLET
Sbjct: 301 VLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLGDQLLEESEVPKLSYLRCIVLET 360
Query: 361 LRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEGG 420
LRLYPAGPLLLPHKSSADCTLSGY+VPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEGG
Sbjct: 361 LRLYPAGPLLLPHKSSADCTLSGYNVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEGG 420
Query: 421 DGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGKGVSM 480
D EANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGKGVSM
Sbjct: 421 DSEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGKGVSM 480
Query: 481 PKAEPLEALCKPRPIASKLLL 502
PKAEPLEALCKPRPIASKLLL
Sbjct: 481 PKAEPLEALCKPRPIASKLLL 501
BLAST of Carg03795 vs. NCBI nr
Match:
XP_023513972.1 (isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 981.5 bits (2536), Expect = 2.7e-282
Identity = 492/501 (98.20%), Postives = 496/501 (99.00%), Query Frame = 0
Query: 1 MDDSTFLYSVLCIALVVAISYALKQKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQ 60
M+D+TFLYSVL IAL+VAISYALK KSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQ
Sbjct: 1 MEDTTFLYSVLGIALLVAISYALKPKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQ 60
Query: 61 KYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYG 120
KYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYG
Sbjct: 61 KYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYG 120
Query: 121 DHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMVKELTL 180
DHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQ SRDDFARVEFKSMVKELTL
Sbjct: 121 DHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVKELTL 180
Query: 181 NITMRMVAGKRYYGEETDESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQGFMKR 240
NITMRMVAGKRYYGEETDESSEA TFRAIVDELFEFMLAQYPADFLPILKYID QGFMKR
Sbjct: 181 NITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQGFMKR 240
Query: 241 AKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILTM 300
AKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILTM
Sbjct: 241 AKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILTM 300
Query: 301 VLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLGDQLLEESEVPKLSYLRCIVLET 360
VLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLGDQLLEESEVPKLSYLRCIVLET
Sbjct: 301 VLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLGDQLLEESEVPKLSYLRCIVLET 360
Query: 361 LRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEGG 420
LRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEGG
Sbjct: 361 LRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEGG 420
Query: 421 DGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGKGVSM 480
DGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGK+VDMTEGKGVSM
Sbjct: 421 DGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKDVDMTEGKGVSM 480
Query: 481 PKAEPLEALCKPRPIASKLLL 502
PKAEPLEALCKPRPIASKLLL
Sbjct: 481 PKAEPLEALCKPRPIASKLLL 501
BLAST of Carg03795 vs. NCBI nr
Match:
XP_038907094.1 (cytochrome P450 81Q32-like [Benincasa hispida])
HSP 1 Score: 842.8 bits (2176), Expect = 1.5e-240
Identity = 421/507 (83.04%), Postives = 462/507 (91.12%), Query Frame = 0
Query: 1 MDDSTFLY---SVLCIALVVA-ISYALKQKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLL 60
M+DS Y S+L I +V+ I + LK KNLPP PPSIPIIGHLHLLK PVHR+L
Sbjct: 1 MEDSILAYSAISILSIGIVLGIIKHLLKSNCYKNLPPCPPSIPIIGHLHLLKLPVHRTLQ 60
Query: 61 TLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGA 120
TLSQKYGPVLSLRFGSRLVVVVSS+ AAEECFTKND++FANRPDFAVS+CLSYNHTTLGA
Sbjct: 61 TLSQKYGPVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLGA 120
Query: 121 APYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMVK 180
APYGDHWRKLRRVSALEILS+NRLNTNS IRRDEVRR++ K+ + SRDDF +VEFKSMVK
Sbjct: 121 APYGDHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMVK 180
Query: 181 ELTLNITMRMVAGKRYYGEETDESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQG 240
ELTLNITMRMVAGKRYYGEE +SSEARTF+AIV+ELFEF L+ YPADFLPILKYIDIQG
Sbjct: 181 ELTLNITMRMVAGKRYYGEEAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQG 240
Query: 241 FMKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGL 300
FMKRAKKLI RVDAFWQG++DEHR +I N+EMENSMVAHFLKLQESQP+YYTDDIIKGL
Sbjct: 241 FMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKGL 300
Query: 301 ILTMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLG-DQLLEESEVPKLSYLRC 360
ILTM+LGGSDTTAVTIEWAMSNLLNHP+VLKKARAELE+QLG DQLLEE+++ KLSYLRC
Sbjct: 301 ILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLRC 360
Query: 361 IVLETLRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPE 420
IVLETLRLYPAGPLLLPHKSSADCT+ GYDVP DTILLVNAWAIHRDPTLWEDP SF+PE
Sbjct: 361 IVLETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKPE 420
Query: 421 RFE--GGDGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMT 480
RFE G+G+ +SKLMIAFGLGRRACPG+GMAHRVLGL+LGLLIQCFDWKSI+GKEVDMT
Sbjct: 421 RFENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDMT 480
Query: 481 EGKGVSMPKAEPLEALCKPRPIASKLL 501
EG GVSMPKA+PLEALCK R IA+KLL
Sbjct: 481 EGIGVSMPKAQPLEALCKAREIATKLL 507
BLAST of Carg03795 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 559.7 bits (1441), Expect = 3.4e-158
Identity = 279/503 (55.47%), Postives = 376/503 (74.75%), Query Frame = 0
Query: 1 MDDSTFLYSVLCIALV-VAISYALKQKSRKNLPPTPP-SIPIIGHLHLLKPPVHRSLLTL 60
M+ ST LY+ L + L+ +++ + R+NLPP+P ++P+IGHLHL+ +HRSL L
Sbjct: 1 MESSTLLYTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDL 60
Query: 61 SQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAP 120
S+KYG V SL+ G+RLV+VVSS AAEECFTKND+VFANRP F + K + YN+TT+ +P
Sbjct: 61 SKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSP 120
Query: 121 YGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMVKEL 180
YG+HWR LRR++A+EI SA LN +IR DEV++L+ L Q S DF +VE KS + EL
Sbjct: 121 YGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSEL 180
Query: 181 TLNITMRMVAGKRYYGEETDESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQGFM 240
+ N+TMRMVAGKRY+G++ D S EA+ FRA++ E+FE A P DF+P L++ID + +
Sbjct: 181 SFNVTMRMVAGKRYFGQDVD-SDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYE 240
Query: 241 KRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLIL 300
K+ K+ + +DAF Q ++ E R N + +M+ H L LQESQP+YYTD IIKG+I+
Sbjct: 241 KKVSKISQEMDAFLQRLIHESRIN-----KNNVTMIDHLLSLQESQPEYYTDQIIKGIIM 300
Query: 301 TMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLG-DQLLEESEVPKLSYLRCIV 360
++L G+DT+AVT+EWAMS LLNHP L+KAR E+ETQ+G ++L+EE ++PKL+YL I+
Sbjct: 301 VLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNII 360
Query: 361 LETLRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERF 420
ET RL PA P+L+PH+SS DC + GYDVP+ TILLVNAWAIHRDP W++PT F+PER
Sbjct: 361 SETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERH 420
Query: 421 EGGDGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGKG 480
G + E SKLM FG+GRR+CPGSG+A RV+GLTLG LIQCF+WK I ++DM EG G
Sbjct: 421 GGVELEP-SKLM-PFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSG 480
Query: 481 VSMPKAEPLEALCKPRPIASKLL 501
++MPKA+PLEALCKPR I K++
Sbjct: 481 LTMPKAQPLEALCKPRNILHKVV 495
BLAST of Carg03795 vs. ExPASy Swiss-Prot
Match:
P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)
HSP 1 Score: 543.1 bits (1398), Expect = 3.3e-153
Identity = 263/499 (52.71%), Postives = 362/499 (72.55%), Query Frame = 0
Query: 4 STFLYSVLCIALVVAISYALKQKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQKYG 63
S YSV +AL + ++ + KNLPP PPS+PIIG+LH LK P+HR+ LS+KYG
Sbjct: 5 SLLSYSVFYLALFFIFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYG 64
Query: 64 PVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYGDHW 123
V SL FGSRLVVVVSS ++CFTKNDVV ANRP F K + YN+TTLG+ YG+HW
Sbjct: 65 HVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHW 124
Query: 124 RKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMVKELTLNIT 183
R LRR++AL++LS +R+N+ S IRRDE +RL+ +L+ S +FA +E S + ++T N
Sbjct: 125 RNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNI 184
Query: 184 MRMVAGKRYYGEETDES--SEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQGFMKRA 243
MRM++GKRYYGE+ D S EA FR +V EL + A DF+P+L+++D + KR
Sbjct: 185 MRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRL 244
Query: 244 KKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILTMV 303
K + + DAF +G+++EHRT +E N+M+ H L LQ+SQP+YYTD IIKGL L M+
Sbjct: 245 KDISGKTDAFLRGLIEEHRT----KKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAML 304
Query: 304 LGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLG-DQLLEESEVPKLSYLRCIVLET 363
L G+D++AVT+EW+MSNLLNHP VLKK + EL+T +G D+L++ES++PKL+YL+ ++ ET
Sbjct: 305 LAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINET 364
Query: 364 LRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEGG 423
LRLY PLLLPH +S +C + GY VP+DTI+L+NAWAIHRDP LW + T+F+PERFE
Sbjct: 365 LRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFE-- 424
Query: 424 DGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGKGVSM 483
+ + +IAFG+GRRACPG G+A R + +TL LLIQCFDWK I+G ++D+ E G ++
Sbjct: 425 -KKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTL 484
Query: 484 PKAEPLEALCKPRPIASKL 500
K PL+A+CK RP+ +K+
Sbjct: 485 TKLVPLKAMCKSRPVINKV 496
BLAST of Carg03795 vs. ExPASy Swiss-Prot
Match:
Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 534.6 bits (1376), Expect = 1.2e-150
Identity = 265/500 (53.00%), Postives = 354/500 (70.80%), Query Frame = 0
Query: 6 FLYSVLCIALVVAISYALKQKSR--KNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQKYG 65
F YS+L ++ ++ I LK SR KNLPP PP+IPIIG+LH LK P+HR+ TLSQ YG
Sbjct: 3 FYYSLLSLSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYG 62
Query: 66 PVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYGDHW 125
+ SL FGSRLVVVVSS A ECFTKND++ ANRP F K + YN+TTLG+A YGDHW
Sbjct: 63 DIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHW 122
Query: 126 RKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDD---FARVEFKSMVKELTL 185
R LRR++ +++LS NRLN+ +RRDE RL+ KL + + F +VE + + E+T
Sbjct: 123 RNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTF 182
Query: 186 NITMRMVAGKRYYGEETDES--SEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQGFM 245
N MRM++GKRYYG++ D S EA+ FR I+ E+ + A DFLP+L+ +D+
Sbjct: 183 NAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLE 242
Query: 246 KRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLIL 305
KR K++ KR +AF +G+++EHR IH+ +M+ H LKL ESQP+YY+D +IKGLI
Sbjct: 243 KRCKRIAKRSNAFLEGLIEEHRRGNIHSD--GGTMIDHLLKLSESQPEYYSDHLIKGLIQ 302
Query: 306 TMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLG-DQLLEESEVPKLSYLRCIV 365
M+L G+DT+AVTIEW MS LLNHP VLKKA+ EL+TQ+G ++L++E ++ KL YL+ I+
Sbjct: 303 GMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNII 362
Query: 366 LETLRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERF 425
ETLRL+P PLLLPH SS DCT+ ++VP+DTI+L N W IHRDP W D SF+PERF
Sbjct: 363 SETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERF 422
Query: 426 EGGDGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGKG 485
E E ++AFGLGRRACPG +A R +G T+GLLIQCF+W+ +++DM EGKG
Sbjct: 423 E---KEEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKG 482
Query: 486 VSMPKAEPLEALCKPRPIAS 498
++MP PL A+CK PIA+
Sbjct: 483 ITMPMKIPLRAMCKALPIAN 497
BLAST of Carg03795 vs. ExPASy Swiss-Prot
Match:
Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 530.0 bits (1364), Expect = 2.9e-149
Identity = 264/492 (53.66%), Postives = 350/492 (71.14%), Query Frame = 0
Query: 5 TFLYSVLCIALVVAISYALKQKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQKYGP 64
+ + S+ + + + + + +K KNLPP P +PIIG+LH LK P+H + TLSQKYG
Sbjct: 7 SLIISLFFLIITLKVFFNTSRKF-KNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQ 66
Query: 65 VLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYGDHWR 124
+ SL FGSRLVVVVSS+ A+ECFTKND+V ANRP F K + YN+TT+ +PYGDHWR
Sbjct: 67 IFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWR 126
Query: 125 KLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMVKELTLNITM 184
LRR+ ++EILS++RLN+ IRRDE+ RL+ KL+Q S + F VE + M E+T N M
Sbjct: 127 NLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIM 186
Query: 185 RMVAGKRYYGEETDES--SEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQGFMKRAK 244
RMV+GKRYYG + D S EAR FR I+ E+ A DFL L++ D G KR K
Sbjct: 187 RMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLK 246
Query: 245 KLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILTMVL 304
K+ KR DAF QG++DEHR + N+M+ H L Q+SQP+YYTD IIKGL++ M+L
Sbjct: 247 KISKRTDAFLQGLIDEHR----FGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLL 306
Query: 305 GGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLG-DQLLEESEVPKLSYLRCIVLETL 364
G+DT++VTIEWAMSNLLNHP ++KKA+ EL+T +G D+ ++E ++ KL YL+ IV ETL
Sbjct: 307 AGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETL 366
Query: 365 RLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEGGD 424
RL+ A PLL+PH SS D +L GY++P++TIL+VNAW IHRDP LW DPT F+PERFE +
Sbjct: 367 RLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFE-KE 426
Query: 425 GEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGKGVSMP 484
GE N +++FGLGRRACPG ++ R GLTLGLLIQCF+WK I +++DM E KG++
Sbjct: 427 GEVNK--LLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITAG 486
Query: 485 KAEPLEALCKPR 494
K L A+CK R
Sbjct: 487 KKTSLNAMCKVR 490
BLAST of Carg03795 vs. ExPASy Swiss-Prot
Match:
Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)
HSP 1 Score: 523.9 bits (1348), Expect = 2.1e-147
Identity = 254/499 (50.90%), Postives = 356/499 (71.34%), Query Frame = 0
Query: 4 STFLYSVLCIALVVAISYALKQKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQKYG 63
S YS+ +++ I + + KNLPP P S+PIIG+LH LK P++R+ L++KYG
Sbjct: 5 SYLCYSLFYLSIFFIIRLLFQSRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTEKYG 64
Query: 64 PVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYGDHW 123
V+SL FGSRLVVVVSS+ +ECFTKNDVV ANRP F K + YN+TTLG+ YG+HW
Sbjct: 65 NVISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHW 124
Query: 124 RKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMVKELTLNIT 183
R LRR+++L++LS +R+N + IRRDE +RL+ KL++ S FA VE ++T N
Sbjct: 125 RNLRRITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMTFNNI 184
Query: 184 MRMVAGKRYYGEETD--ESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQGFMKRA 243
MRM++GKRYYG++ D E EA FR +V EL + A DF+P+LK++D + KR
Sbjct: 185 MRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRV 244
Query: 244 KKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILTMV 303
K++ ++ D F G++ E R+ +E N+M+ H L +QESQP+YYTD IIKGL L M+
Sbjct: 245 KRIGEKNDVFLSGLLQEQRS----KKERTNTMIDHLLNMQESQPEYYTDTIIKGLCLAML 304
Query: 304 LGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLG-DQLLEESEVPKLSYLRCIVLET 363
L G+D++AVT+EW MSN+LN+P VLKK R E++T +G D+L++ES++PKL+YLR ++ ET
Sbjct: 305 LAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYET 364
Query: 364 LRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEGG 423
LRLY PLLLPH ++ +C + GY VPRDTI+L+NAWAIHRDP W + T+F+PERF
Sbjct: 365 LRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERF--- 424
Query: 424 DGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGKGVSM 483
D + + MIAFG+GRRACPG G+A R + +TL LL+QCFDWK I+ +++DM+E G +M
Sbjct: 425 DKKGELEKMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSERDGFTM 484
Query: 484 PKAEPLEALCKPRPIASKL 500
K PL+A+CK RP+ +K+
Sbjct: 485 TKLLPLKAMCKTRPVVNKV 496
BLAST of Carg03795 vs. ExPASy TrEMBL
Match:
A0A6J1H614 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111460794 PE=3 SV=1)
HSP 1 Score: 987.6 bits (2552), Expect = 1.9e-284
Identity = 495/501 (98.80%), Postives = 495/501 (98.80%), Query Frame = 0
Query: 1 MDDSTFLYSVLCIALVVAISYALKQKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQ 60
MDDSTFLYS LCIALVVAISYALK KS KNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQ
Sbjct: 1 MDDSTFLYSALCIALVVAISYALKPKSHKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQ 60
Query: 61 KYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYG 120
KYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYG
Sbjct: 61 KYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYG 120
Query: 121 DHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMVKELTL 180
DHWRKLRRVSALEILSANRLNTNSAIRRDEVRRL NKLSQ SRDDFARVEFKSMVKELTL
Sbjct: 121 DHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLANKLSQTSRDDFARVEFKSMVKELTL 180
Query: 181 NITMRMVAGKRYYGEETDESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQGFMKR 240
NITMRMVAGKRYYGEETDESSEA TFRAIVDELFEFMLAQYPADFLPILKYIDIQGFMKR
Sbjct: 181 NITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDIQGFMKR 240
Query: 241 AKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILTM 300
AKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILTM
Sbjct: 241 AKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILTM 300
Query: 301 VLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLGDQLLEESEVPKLSYLRCIVLET 360
VLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLGDQLLEESEVPKLSYLRCIVLET
Sbjct: 301 VLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLGDQLLEESEVPKLSYLRCIVLET 360
Query: 361 LRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEGG 420
LRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEGG
Sbjct: 361 LRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEGG 420
Query: 421 DGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGKGVSM 480
DGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGKGVSM
Sbjct: 421 DGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGKGVSM 480
Query: 481 PKAEPLEALCKPRPIASKLLL 502
PKAEPLEALCKPRPIASKLLL
Sbjct: 481 PKAEPLEALCKPRPIASKLLL 501
BLAST of Carg03795 vs. ExPASy TrEMBL
Match:
A0A6J1KS03 (isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111498126 PE=3 SV=1)
HSP 1 Score: 984.9 bits (2545), Expect = 1.2e-283
Identity = 493/501 (98.40%), Postives = 497/501 (99.20%), Query Frame = 0
Query: 1 MDDSTFLYSVLCIALVVAISYALKQKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQ 60
MDDSTFLYSVLCIALVVAISYALK KSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQ
Sbjct: 1 MDDSTFLYSVLCIALVVAISYALKPKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQ 60
Query: 61 KYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYG 120
KYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYG
Sbjct: 61 KYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYG 120
Query: 121 DHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMVKELTL 180
DHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQ SRDDFARVEFKSMVKELTL
Sbjct: 121 DHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVKELTL 180
Query: 181 NITMRMVAGKRYYGEETDESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQGFMKR 240
NITMRMVAGKRYYGEET+ESSEARTFRAIV+ELFEFMLAQYPADFLPILKYID QGFMKR
Sbjct: 181 NITMRMVAGKRYYGEETNESSEARTFRAIVNELFEFMLAQYPADFLPILKYIDFQGFMKR 240
Query: 241 AKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILTM 300
AKKLIKRVDAFWQGIVDEHRTNQ+HNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILTM
Sbjct: 241 AKKLIKRVDAFWQGIVDEHRTNQVHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILTM 300
Query: 301 VLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLGDQLLEESEVPKLSYLRCIVLET 360
VLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLGDQLLEESEVPKLSYLRCIVLET
Sbjct: 301 VLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLGDQLLEESEVPKLSYLRCIVLET 360
Query: 361 LRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEGG 420
LRLYPAGPLLLPHKSSADCTLSGY+VPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEGG
Sbjct: 361 LRLYPAGPLLLPHKSSADCTLSGYNVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEGG 420
Query: 421 DGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGKGVSM 480
D EANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGKGVSM
Sbjct: 421 DSEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGKGVSM 480
Query: 481 PKAEPLEALCKPRPIASKLLL 502
PKAEPLEALCKPRPIASKLLL
Sbjct: 481 PKAEPLEALCKPRPIASKLLL 501
BLAST of Carg03795 vs. ExPASy TrEMBL
Match:
A0A5D3DLU7 (Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G001700 PE=3 SV=1)
HSP 1 Score: 821.2 bits (2120), Expect = 2.3e-234
Identity = 410/508 (80.71%), Postives = 455/508 (89.57%), Query Frame = 0
Query: 1 MDDSTFLY---SVLCIALVV--AISYALKQKSRKNLPPTPPSIPIIGHLHLLKPPVHRSL 60
M+DS Y S+L I +VV I Y LK S KNLPP+PPSIPIIGHLHLLK PVHR+L
Sbjct: 1 MEDSILQYSAFSILSIGVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60
Query: 61 LTLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLG 120
TLSQKYGPVLSLRFGSRLVVVV+S++AAEECFTKNDV+FANRP+FAVS CLSYN TTLG
Sbjct: 61 QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120
Query: 121 AAPYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMV 180
AAPYGDHWRKLRRVS LEIL ++RLN N IRRDEVRR V K+ + SRD F +VEFK +V
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAVKKIFEVSRDGFGKVEFKPLV 180
Query: 181 KELTLNITMRMVAGKRYYGEETDESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQ 240
KELT+NITMRMVAGKRYYGEE +SSEARTF+AIV ELFEF L+ YPADFLPILKYIDIQ
Sbjct: 181 KELTMNITMRMVAGKRYYGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240
Query: 241 GFMKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKG 300
GFMKRAKKLI RVDAFWQG++DEHR +I N+EM+N MVAHFLKLQE+QP YYTDDIIKG
Sbjct: 241 GFMKRAKKLIARVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300
Query: 301 LILTMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQL-GDQLLEESEVPKLSYLR 360
LILTM+LGGSDTTAVTIEWAMSNLLNHP+VLKKARAE+E+QL GD+LLEE+++ KLSYLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLDGDKLLEETDLSKLSYLR 360
Query: 361 CIVLETLRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
CIVLETLRLYPAGPLLLPHKSSADC++SGY++PRDTILLVNAWAIHRDPTLWEDPTSFRP
Sbjct: 361 CIVLETLRLYPAGPLLLPHKSSADCSISGYNIPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
Query: 421 ERFE--GGDGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDM 480
ERFE G+G+AN+KLMIAFGLGRRACPG+GMAHRV+GL LG LIQCFDWKSIDGKEVDM
Sbjct: 421 ERFENNNGEGDANNKLMIAFGLGRRACPGTGMAHRVMGLMLGTLIQCFDWKSIDGKEVDM 480
Query: 481 TEGKGVSMPKAEPLEALCKPRPIASKLL 501
EGKGVSMPKA+PLEALCK R IA+KLL
Sbjct: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 508
BLAST of Carg03795 vs. ExPASy TrEMBL
Match:
A0A1S3CAW8 (isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103498910 PE=3 SV=1)
HSP 1 Score: 821.2 bits (2120), Expect = 2.3e-234
Identity = 410/508 (80.71%), Postives = 455/508 (89.57%), Query Frame = 0
Query: 1 MDDSTFLY---SVLCIALVV--AISYALKQKSRKNLPPTPPSIPIIGHLHLLKPPVHRSL 60
M+DS Y S+L I +VV I Y LK S KNLPP+PPSIPIIGHLHLLK PVHR+L
Sbjct: 1 MEDSILQYSAFSILSIGVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60
Query: 61 LTLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLG 120
TLSQKYGPVLSLRFGSRLVVVV+S++AAEECFTKNDV+FANRP+FAVS CLSYN TTLG
Sbjct: 61 QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120
Query: 121 AAPYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMV 180
AAPYGDHWRKLRRVS LEIL ++RLN N IRRDEVRR V K+ + SRD F +VEFK +V
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAVKKIFEVSRDGFGKVEFKPLV 180
Query: 181 KELTLNITMRMVAGKRYYGEETDESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQ 240
KELT+NITMRMVAGKRYYGEE +SSEARTF+AIV ELFEF L+ YPADFLPILKYIDIQ
Sbjct: 181 KELTMNITMRMVAGKRYYGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240
Query: 241 GFMKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKG 300
GFMKRAKKLI RVDAFWQG++DEHR +I N+EM+N MVAHFLKLQE+QP YYTDDIIKG
Sbjct: 241 GFMKRAKKLIARVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300
Query: 301 LILTMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQL-GDQLLEESEVPKLSYLR 360
LILTM+LGGSDTTAVTIEWAMSNLLNHP+VLKKARAE+E+QL GD+LLEE+++ KLSYLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLDGDKLLEETDLSKLSYLR 360
Query: 361 CIVLETLRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
CIVLETLRLYPAGPLLLPHKSSADC++SGY++PRDTILLVNAWAIHRDPTLWEDPTSFRP
Sbjct: 361 CIVLETLRLYPAGPLLLPHKSSADCSISGYNIPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
Query: 421 ERFE--GGDGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDM 480
ERFE G+G+AN+KLMIAFGLGRRACPG+GMAHRV+GL LG LIQCFDWKSIDGKEVDM
Sbjct: 421 ERFENNNGEGDANNKLMIAFGLGRRACPGTGMAHRVMGLMLGTLIQCFDWKSIDGKEVDM 480
Query: 481 TEGKGVSMPKAEPLEALCKPRPIASKLL 501
EGKGVSMPKA+PLEALCK R IA+KLL
Sbjct: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 508
BLAST of Carg03795 vs. ExPASy TrEMBL
Match:
A0A097IYP1 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa6G088160 PE=2 SV=1)
HSP 1 Score: 800.8 bits (2067), Expect = 3.2e-228
Identity = 397/508 (78.15%), Postives = 450/508 (88.58%), Query Frame = 0
Query: 1 MDDSTFLY---SVLCIALVV--AISYALKQKSRKNLPPTPPSIPIIGHLHLLKPPVHRSL 60
M+DS Y S+L IA+VV I Y LK S KNLPP+PPSIPIIGHLHLLK PVHR+L
Sbjct: 1 MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60
Query: 61 LTLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLG 120
TLSQKYGPVLSLRFGSR VVVV+S++AAEECF KND++FANRP+FAVS CLSYN TTLG
Sbjct: 61 QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG 120
Query: 121 AAPYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMV 180
AAPYGDHWRKLRRVS LEIL ++RLN N IRRDEVRR + K+ + SRD F +VEFK +V
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV 180
Query: 181 KELTLNITMRMVAGKRYYGEETDESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQ 240
KELT+N+TMRMVAGKRY+GEE +SSEARTF+AIV ELFEF L+ YPADFLPILKYIDIQ
Sbjct: 181 KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240
Query: 241 GFMKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKG 300
GFMKRAKKLI RVDAFWQG++DEHR +I N+EM+N MVAHFLKLQE+QP YYTDDIIKG
Sbjct: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300
Query: 301 LILTMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQL-GDQLLEESEVPKLSYLR 360
LILTM+LGGSDTTAVTIEWAMSNLLNHP+VLKKARAE+E+QL GD+L+EE+++ KL+YLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLR 360
Query: 361 CIVLETLRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
I+LETLRLYPAGPLLLPHKSSADC +SGYD+PRDT+LLVNAWAIHRDP LWEDPTSF+P
Sbjct: 361 YIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKP 420
Query: 421 ERFE--GGDGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDM 480
ERFE G+G+ N+KLMIAFGLGRRACPG+GMA+RV+GL LG LIQCFDWKSIDGKEVDM
Sbjct: 421 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDM 480
Query: 481 TEGKGVSMPKAEPLEALCKPRPIASKLL 501
EGKGVSMPKA+PLEALCK R IA+KLL
Sbjct: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 508
BLAST of Carg03795 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 535.0 bits (1377), Expect = 6.3e-152
Identity = 272/504 (53.97%), Postives = 373/504 (74.01%), Query Frame = 0
Query: 1 MDDSTFLYSVLCIALVVAISYAL-KQKSRKNLPPTPP-SIPIIGHLHLLKPPVHRSLLTL 60
M+ T ++S+L + V+++ Y + K K + NLPP+P S+P+IGHL LLKPP+HR+ L+L
Sbjct: 1 METKTLIFSILFV--VLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSL 60
Query: 61 SQKY--GPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGA 120
SQ P+ SLR G+RLV V SS AEECFTKNDVV ANRP+F ++K ++Y++TT+ A
Sbjct: 61 SQSLNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIA 120
Query: 121 APYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMVK 180
A YGDHWR LRR+ ++EI S +RLN+ +IR+DE+RRLV +LS+ +F +V+ KSM+
Sbjct: 121 ASYGDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLS 180
Query: 181 ELTLNITMRMVAGKRYYGEETDESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQG 240
+LT N +RMVAGKRYYG+ ++ EA+ R ++ ++ A D+LP+L+ +
Sbjct: 181 DLTFNNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRL--VSD 240
Query: 241 FMKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGL 300
+ R KKL R+D F QG+VDE R +E N+M+ H L LQESQP Y+TD IIKG
Sbjct: 241 YETRVKKLAGRLDEFLQGLVDEKR----EAKEKGNTMIDHLLTLQESQPDYFTDRIIKGN 300
Query: 301 ILTMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLG-DQLLEESEVPKLSYLRC 360
+L ++L G+DT+AVT+EWA+SN+LNHP VL KAR E++ ++G D+L++ES++ L YL+
Sbjct: 301 MLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQN 360
Query: 361 IVLETLRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPE 420
IV ETLRLYPA P+LLPH +S DC ++GYD+PR TILL N WAIHRDP LW+DP SF+PE
Sbjct: 361 IVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPE 420
Query: 421 RFEGGDGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEG 480
RFE +GEA ++ FGLGRRACPGSG+AHR++ LTLG LIQC +W+ I G+EVDM+EG
Sbjct: 421 RFE-KEGEAQK--LMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKI-GEEVDMSEG 480
Query: 481 KGVSMPKAEPLEALCKPRPIASKL 500
KGV+MPKA+PLEA+C+ RP K+
Sbjct: 481 KGVTMPKAKPLEAMCRARPSVVKI 492
BLAST of Carg03795 vs. TAIR 10
Match:
AT4G37310.1 (cytochrome P450, family 81, subfamily H, polypeptide 1 )
HSP 1 Score: 529.6 bits (1363), Expect = 2.7e-150
Identity = 267/508 (52.56%), Postives = 363/508 (71.46%), Query Frame = 0
Query: 3 DSTFLYSVLCIALVVAISYALKQKSRKNLPPTPP-SIPIIGHLHLLKPP-VHRSLLTLSQ 62
D L +A+ + L K KNLPP+P PIIGHLHLLK P +HR+L LS
Sbjct: 2 DCILLILTTLVAIFIVKIVLLVTKPNKNLPPSPNICFPIIGHLHLLKKPLLHRTLSHLSH 61
Query: 63 KYGPVLSLRFGSRLVVVVSSIDAAEECF-TKNDVVFANRPDFAVSKCLSYNHTTLGAAPY 122
GPV SLR GSRL V++SS AAEECF TKND+V ANRP F + K ++Y++T++ APY
Sbjct: 62 SLGPVFSLRLGSRLAVIISSPTAAEECFLTKNDIVLANRPRFIMGKYVAYDYTSMVTAPY 121
Query: 123 GDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMVKELT 182
GDHWR LRR++ALE+ S NRLN ++ IR DEV+ L+ KL S + A+VE + ++ LT
Sbjct: 122 GDHWRNLRRITALEVFSTNRLNASAEIRHDEVKMLLQKLHDLSVERPAKVELRQLLTGLT 181
Query: 183 LNITMRMVAGKRYYGEET-DESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQGFM 242
LN+ MRM+ GKR++ E+ ++ + FR +V E+ E A PADFLP L++ D +G +
Sbjct: 182 LNVIMRMMTGKRFFEEDDGGKAGISLEFRELVAEILELSAADNPADFLPALRWFDYKGLV 241
Query: 243 KRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLIL 302
KRAK++ +R+D+ QG +DEHR N+ E +N+M+AH L QE +P Y+D IKGLIL
Sbjct: 242 KRAKRIGERMDSLLQGFLDEHRANK-DRLEFKNTMIAHLLDSQEKEPHNYSDQTIKGLIL 301
Query: 303 TMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQL----GDQLLEESEVPKLSYLR 362
MV+GG+DT+A+T+EWAMSNLLNHP +L+ R ++TQ+ +LL+E ++ ++YL+
Sbjct: 302 MMVVGGTDTSALTVEWAMSNLLNHPQILETTRQNIDTQMETSSSRRLLKEEDLVNMNYLK 361
Query: 363 CIVLETLRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRP 422
+V ETLRLYP PL++PH S+DC + G++VPRDTI+LVN WAIHRDP++W+DPTSF+P
Sbjct: 362 NVVSETLRLYPVAPLMVPHVPSSDCVIGGFNVPRDTIVLVNLWAIHRDPSVWDDPTSFKP 421
Query: 423 ERFEGGD--GEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDM 482
ERFEG D G N K+M FGLGRRACPG +A+RV+GL LG +IQCF+W+S G +VDM
Sbjct: 422 ERFEGSDQFGHYNGKMM-PFGLGRRACPGLSLANRVVGLLLGSMIQCFEWESGSGGQVDM 481
Query: 483 TEGKGVSMPKAEPLEALCKPRPIASKLL 501
TEG G+S+PKAEPL C+ R +AS+LL
Sbjct: 482 TEGPGLSLPKAEPLVVTCRTREMASELL 507
BLAST of Carg03795 vs. TAIR 10
Match:
AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )
HSP 1 Score: 520.4 bits (1339), Expect = 1.6e-147
Identity = 267/500 (53.40%), Postives = 359/500 (71.80%), Query Frame = 0
Query: 5 TFLYSVLCIALVVAISYALKQKSRKNLPPTPP-SIPIIGHLHLLKPPVHRSLLTLSQKYG 64
TF + VL + ++ + K + NLPP+P ++P+IGHL LLKPP+HR L++SQ G
Sbjct: 9 TFCFIVLSLIFLIG-----RIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLG 68
Query: 65 --PVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYGD 124
P++SLR G+RL+ VVSS AEECFTKNDV+ ANR +K +SY ++T+ +A Y +
Sbjct: 69 DAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSE 128
Query: 125 HWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMVKELTLN 184
HWR LRR+ ALEI SA+RLN+ S+IRRDE+RRL+ +L + S F +VE KSM +LT N
Sbjct: 129 HWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTFN 188
Query: 185 ITMRMVAGKRYYGEETDESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQGFMKRA 244
+RM+AGK YYG+ ++ EA+ R ++ E AD++PIL +I R
Sbjct: 189 NIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS--ETRI 248
Query: 245 KKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILTMV 304
KKL R+D F QG+VDE R + ++ EN+MV H L LQE+QP+YY D IIKG +L+++
Sbjct: 249 KKLAGRLDEFLQGLVDEKREGK---EKKENTMVDHLLCLQETQPEYYMDRIIKGTMLSLI 308
Query: 305 LGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLG-DQLLEESEVPKLSYLRCIVLET 364
GG+DTTAVT+EWA+S+LLN+P VL KAR E++ +G D+LLEES++P L YL+ IV ET
Sbjct: 309 AGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSET 368
Query: 365 LRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEGG 424
LRLYPA P+LLPH +S DC + GYD+PR T+LL NAWAIHRDP LW+DPTSF+PERFE
Sbjct: 369 LRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFE-- 428
Query: 425 DGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGKGVSM 484
E +K ++ FGLGRRACPGSG+A R++ L+LG LIQCF+W+ I +EVDMTEG G++M
Sbjct: 429 -KEGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTM 488
Query: 485 PKAEPLEALCKPRPIASKLL 501
PKA PLEA+C+ R K+L
Sbjct: 489 PKARPLEAMCRARDFVGKIL 495
BLAST of Carg03795 vs. TAIR 10
Match:
AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )
HSP 1 Score: 516.9 bits (1330), Expect = 1.8e-146
Identity = 271/505 (53.66%), Postives = 364/505 (72.08%), Query Frame = 0
Query: 1 MDDSTFLYSVLCIALVVAISYALKQKSRKNLPPTPP-SIPIIGHLHLLKPPVHRSLLTLS 60
M+ T +++ L I+L + + K R NLPP+P ++P+IGHL LLKPP+HR L++S
Sbjct: 1 MEGQTLIFTFLFISLSLTFIIG-RIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVS 60
Query: 61 QKYG--PVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAA 120
+ G P++SLR G+RLV VVSS AEECFTKNDVV ANR + SK +SY TT+ A
Sbjct: 61 ESLGDAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTA 120
Query: 121 PYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMVKE 180
YGDHWR LRR+ A+EI SA+RLN+ S+IRRDE+ RL+ LS+ S +F +VE KSM
Sbjct: 121 SYGDHWRNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSN 180
Query: 181 LTLNITMRMVAGKRYYGEETDESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQGF 240
LT N +RM+AGK YYG+ ++ EA+ R ++ E A AD+LPIL + I G
Sbjct: 181 LTFNNIIRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTW--ITGS 240
Query: 241 MKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLI 300
KR KK+ R+D F QG+VDE R + ++ +N+MV H L LQE+QP+YYTD+IIKG++
Sbjct: 241 EKRIKKIASRLDEFLQGLVDERREGK---EKRQNTMVDHLLCLQETQPEYYTDNIIKGIM 300
Query: 301 LTMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLG-DQLLEESEVPKLSYLRCI 360
L+++L G+DT+AVT+EW +S LLNHP +L KAR E++ ++G ++L+EES++ L YL+ I
Sbjct: 301 LSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNI 360
Query: 361 VLETLRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPER 420
V E+LRLYPA PLL+PH +S DC + GY +PR T+LL NAWAIHRDP +W+DPTSF+PER
Sbjct: 361 VSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPER 420
Query: 421 FEGGDGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTE-G 480
FE +GEA ++ FGLGRRACPGSG+A R+ LT+G LIQCF+W+ I +EVDMTE G
Sbjct: 421 FE-KEGEAQK--LLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGG 480
Query: 481 KGVSMPKAEPLEALCKPRPIASKLL 501
GV MPKA PL A+CK RP+ K+L
Sbjct: 481 GGVIMPKAIPLVAMCKARPVVGKIL 496
BLAST of Carg03795 vs. TAIR 10
Match:
AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )
HSP 1 Score: 515.0 bits (1325), Expect = 6.8e-146
Identity = 265/504 (52.58%), Postives = 354/504 (70.24%), Query Frame = 0
Query: 1 MDDSTFLYSVLCIALVVAISYALKQKSRKNLPPTPP-SIPIIGHLHLLKPPVHRSLLTLS 60
MD + L +AI + K + NLPP+P S+P+IGHLHLLKPP+HR+ L+LS
Sbjct: 1 MDLNQILILSFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLS 60
Query: 61 QKYG--PVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAA 120
+ G PV LR G+RLV V+SS AEECFTKNDVV ANRP F +SK L YN T L +A
Sbjct: 61 KSIGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSA 120
Query: 121 PYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMVKE 180
YGDHWR LRR++A+EI S +RLN+ IR+DE+RRL++ LS+ S F VE K+++
Sbjct: 121 SYGDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTN 180
Query: 181 LTLNITMRMVAGKRYYGEETDESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQGF 240
L N T+RM+AGKRY+GE+ D +A+ + +V E A P D+L IL++ + +
Sbjct: 181 LASNTTIRMLAGKRYFGEDND---DAKLVKNLVSEAVTSAGAGNPIDYLSILRW--VSSY 240
Query: 241 MKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLI 300
KR K L R D F Q +VDE R +E +M+ H L LQ+ QP YYTD IIKG+I
Sbjct: 241 EKRIKNLGNRFDTFLQKLVDEKRA----EKEKGETMIDHLLALQDIQPDYYTDVIIKGII 300
Query: 301 LTMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLG-DQLLEESEVPKLSYLRCI 360
LT+++ G+DT++VT+EWAMSNLLNHP +LKKAR E++ ++G D+L++ES++ LSYL+ I
Sbjct: 301 LTLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSI 360
Query: 361 VLETLRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPER 420
VLETLR+YPA PLLLPH SS DC + GYD+P T++L NAWA+HRDP +WEDP F+PER
Sbjct: 361 VLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPER 420
Query: 421 FEGGDGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMTEGK 480
FE +GEA +I+FG+GRRACPG+G+AHR++ LG L+QCF+W+ + VDMTE K
Sbjct: 421 FE-KEGEAEK--LISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDK 480
Query: 481 GVSMPKAEPLEALCKPRPIASKLL 501
G ++PKA PL A+CK R I KL+
Sbjct: 481 GATLPKAIPLRAMCKARSIVDKLI 492
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
W8JMU7 | 3.4e-158 | 55.47 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
P93147 | 3.3e-153 | 52.71 | Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2 | [more] |
Q6WNQ9 | 1.2e-150 | 53.00 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... | [more] |
Q6WNQ8 | 2.9e-149 | 53.66 | Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1 | [more] |
Q6WNR0 | 2.1e-147 | 50.90 | Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1H614 | 1.9e-284 | 98.80 | isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111460794 PE=... | [more] |
A0A6J1KS03 | 1.2e-283 | 98.40 | isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111498126 PE=3 ... | [more] |
A0A5D3DLU7 | 2.3e-234 | 80.71 | Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... | [more] |
A0A1S3CAW8 | 2.3e-234 | 80.71 | isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103498910 PE=3 SV=1 | [more] |
A0A097IYP1 | 3.2e-228 | 78.15 | Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa6G088160 PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
AT4G37370.1 | 6.3e-152 | 53.97 | cytochrome P450, family 81, subfamily D, polypeptide 8 | [more] |
AT4G37310.1 | 2.7e-150 | 52.56 | cytochrome P450, family 81, subfamily H, polypeptide 1 | [more] |
AT4G37360.1 | 1.6e-147 | 53.40 | cytochrome P450, family 81, subfamily D, polypeptide 2 | [more] |
AT4G37340.1 | 1.8e-146 | 53.66 | cytochrome P450, family 81, subfamily D, polypeptide 3 | [more] |
AT4G37330.1 | 6.8e-146 | 52.58 | cytochrome P450, family 81, subfamily D, polypeptide 4 | [more] |