Carg03639 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg03639
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionEukaryotic aspartyl protease family protein
LocationCarg_Chr14: 1522514 .. 1523884 (+)
RNA-Seq ExpressionCarg03639
SyntenyCarg03639
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGCCTCCTCCCCTTCTCTGTTTCTTCTACATTCTCCTCCTCTCCTCTGTTTCCGCCATTGCCGACACCAACCCCATCACCCTCCCTCTCTCCTCCTTCCCCCACCATTCTTCTTCAGATCCACTCCAAACTCTCAATTTCCTCGCCTCTGCTTCCCAAAACAGAGCCCATCAAATCAAAGCCCCCAAATCAAAATCCAACTCTGTCTCCAAGTCCCCTCTCTCCCCCCACAGCTATGGAGCTTACTCTACTCCACTCAGCTTCGGTACCCCATCCCAGACTCTGCATTTGATCTTCGATACAGGTAGTAGTCTCGTTTGGCTCCCTTGCACTTCCAAATATCTCTGTTCCGAGTGTTCTTTCCCCAAAATAGATCCCGCCCGAATCCCCAGATTTATCCCCAAATTGTCTTCCTCTTCCAAGCTTGTCGGTTGCCAGAATCCCAAATGTGCCTGGATTTTTGGCCCCGATGTCAAATCTCAGTGCCGGAGTTGTAACCCCAAAACAGAGAACTGTACCCAAACCTGCCCAGCTTATGTTGTTCAATACGGTTCCGGTTCCACGGCGGGGCTTTTGCTATCGGAAACGCTTGATTTTCCCAATAAGAAAATCACCAATTTTGTTGTTGGGTGCTCGTTTTTGTCGATCCACCAGCCCAGTGGGATCGCCGGATTCGGCCGAGGATCCGAATCGCTCCCCTCGCAAATGGGTCTCAAGAAATTTGCCTACTGCCTCGCGTCTCGAAAATTCGACGACTCCCCTCATGCCGGCGAGCTGATTCTAGATTCCAGCGGAGCCAAGACCAGCGGTCTCACCTACACGCCGTTCCGACAGAACCCCTCCGTTTCTAACCACGCTTACAAAGAATACTATTACTTAACCATACGCAAAATCTTCGTTGGAAACAAGGCCGTGAAGGTGCCGTACAAGTATCTGGTGCCCGGCCCCGACGGAAACGGTGGATCTATCATCGATTCCGGCTCCACCTTCACGTTTATGGACAAACCGGTGTTCGAGGCGGTGGCGCAAGAAATCGAGAAGCAGTTGGCGAACCGGACGAGAGCCACGGATGTAGAATCTCTCACGGGATTACGGCCGTGTTTCGACATTTCGAAGGACAAATCCGTGGAGTTTCCGGAGTTGACTTTCCATTTAAAAGGCGGAGCGAAATGGGCTCTGCCGTTGAGTAACTATTTCGCTTTAGTCAGTAGCTCCGGCGTGGCGTGTTTGACAGTTGTGACGCATAAGGCGGCGGAAAGCGGAGGTGGGCCGTCTATAATTCTTGGGGCTTTTCAGCAGCAGAATTTCTATGTCGAGTATGATTTGGTGAACCAAAAAATAGGCTTTCGTCAACAGACTTGCAGTTAG

mRNA sequence

ATGGCGCCTCCTCCCCTTCTCTGTTTCTTCTACATTCTCCTCCTCTCCTCTGTTTCCGCCATTGCCGACACCAACCCCATCACCCTCCCTCTCTCCTCCTTCCCCCACCATTCTTCTTCAGATCCACTCCAAACTCTCAATTTCCTCGCCTCTGCTTCCCAAAACAGAGCCCATCAAATCAAAGCCCCCAAATCAAAATCCAACTCTGTCTCCAAGTCCCCTCTCTCCCCCCACAGCTATGGAGCTTACTCTACTCCACTCAGCTTCGGTACCCCATCCCAGACTCTGCATTTGATCTTCGATACAGGTAGTAGTCTCGTTTGGCTCCCTTGCACTTCCAAATATCTCTGTTCCGAGTGTTCTTTCCCCAAAATAGATCCCGCCCGAATCCCCAGATTTATCCCCAAATTGTCTTCCTCTTCCAAGCTTGTCGGTTGCCAGAATCCCAAATGTGCCTGGATTTTTGGCCCCGATGTCAAATCTCAGTGCCGGAGTTGTAACCCCAAAACAGAGAACTGTACCCAAACCTGCCCAGCTTATGTTGTTCAATACGGTTCCGGTTCCACGGCGGGGCTTTTGCTATCGGAAACGCTTGATTTTCCCAATAAGAAAATCACCAATTTTGTTGTTGGGTGCTCGTTTTTGTCGATCCACCAGCCCAGTGGGATCGCCGGATTCGGCCGAGGATCCGAATCGCTCCCCTCGCAAATGGGTCTCAAGAAATTTGCCTACTGCCTCGCGTCTCGAAAATTCGACGACTCCCCTCATGCCGGCGAGCTGATTCTAGATTCCAGCGGAGCCAAGACCAGCGGTCTCACCTACACGCCGTTCCGACAGAACCCCTCCGTTTCTAACCACGCTTACAAAGAATACTATTACTTAACCATACGCAAAATCTTCGTTGGAAACAAGGCCGTGAAGGTGCCGTACAAGTATCTGGTGCCCGGCCCCGACGGAAACGGTGGATCTATCATCGATTCCGGCTCCACCTTCACGTTTATGGACAAACCGGTGTTCGAGGCGGTGGCGCAAGAAATCGAGAAGCAGTTGGCGAACCGGACGAGAGCCACGGATGTAGAATCTCTCACGGGATTACGGCCGTGTTTCGACATTTCGAAGGACAAATCCGTGGAGTTTCCGGAGTTGACTTTCCATTTAAAAGGCGGAGCGAAATGGGCTCTGCCGTTGAGTAACTATTTCGCTTTAGTCAGTAGCTCCGGCGTGGCGTGTTTGACAGTTGTGACGCATAAGGCGGCGGAAAGCGGAGGTGGGCCGTCTATAATTCTTGGGGCTTTTCAGCAGCAGAATTTCTATGTCGAGTATGATTTGGTGAACCAAAAAATAGGCTTTCGTCAACAGACTTGCAGTTAG

Coding sequence (CDS)

ATGGCGCCTCCTCCCCTTCTCTGTTTCTTCTACATTCTCCTCCTCTCCTCTGTTTCCGCCATTGCCGACACCAACCCCATCACCCTCCCTCTCTCCTCCTTCCCCCACCATTCTTCTTCAGATCCACTCCAAACTCTCAATTTCCTCGCCTCTGCTTCCCAAAACAGAGCCCATCAAATCAAAGCCCCCAAATCAAAATCCAACTCTGTCTCCAAGTCCCCTCTCTCCCCCCACAGCTATGGAGCTTACTCTACTCCACTCAGCTTCGGTACCCCATCCCAGACTCTGCATTTGATCTTCGATACAGGTAGTAGTCTCGTTTGGCTCCCTTGCACTTCCAAATATCTCTGTTCCGAGTGTTCTTTCCCCAAAATAGATCCCGCCCGAATCCCCAGATTTATCCCCAAATTGTCTTCCTCTTCCAAGCTTGTCGGTTGCCAGAATCCCAAATGTGCCTGGATTTTTGGCCCCGATGTCAAATCTCAGTGCCGGAGTTGTAACCCCAAAACAGAGAACTGTACCCAAACCTGCCCAGCTTATGTTGTTCAATACGGTTCCGGTTCCACGGCGGGGCTTTTGCTATCGGAAACGCTTGATTTTCCCAATAAGAAAATCACCAATTTTGTTGTTGGGTGCTCGTTTTTGTCGATCCACCAGCCCAGTGGGATCGCCGGATTCGGCCGAGGATCCGAATCGCTCCCCTCGCAAATGGGTCTCAAGAAATTTGCCTACTGCCTCGCGTCTCGAAAATTCGACGACTCCCCTCATGCCGGCGAGCTGATTCTAGATTCCAGCGGAGCCAAGACCAGCGGTCTCACCTACACGCCGTTCCGACAGAACCCCTCCGTTTCTAACCACGCTTACAAAGAATACTATTACTTAACCATACGCAAAATCTTCGTTGGAAACAAGGCCGTGAAGGTGCCGTACAAGTATCTGGTGCCCGGCCCCGACGGAAACGGTGGATCTATCATCGATTCCGGCTCCACCTTCACGTTTATGGACAAACCGGTGTTCGAGGCGGTGGCGCAAGAAATCGAGAAGCAGTTGGCGAACCGGACGAGAGCCACGGATGTAGAATCTCTCACGGGATTACGGCCGTGTTTCGACATTTCGAAGGACAAATCCGTGGAGTTTCCGGAGTTGACTTTCCATTTAAAAGGCGGAGCGAAATGGGCTCTGCCGTTGAGTAACTATTTCGCTTTAGTCAGTAGCTCCGGCGTGGCGTGTTTGACAGTTGTGACGCATAAGGCGGCGGAAAGCGGAGGTGGGCCGTCTATAATTCTTGGGGCTTTTCAGCAGCAGAATTTCTATGTCGAGTATGATTTGGTGAACCAAAAAATAGGCTTTCGTCAACAGACTTGCAGTTAG

Protein sequence

MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQIKAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSECSFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIFVGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVESLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAESGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS
Homology
BLAST of Carg03639 vs. NCBI nr
Match: KAG7017489.1 (Aspartyl protease family protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 925.6 bits (2391), Expect = 1.6e-265
Identity = 456/456 (100.00%), Postives = 456/456 (100.00%), Query Frame = 0

Query: 1   MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
           MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI
Sbjct: 1   MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60

Query: 61  KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
           KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC
Sbjct: 61  KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120

Query: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
           SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
           KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF
Sbjct: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300

Query: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
           VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE
Sbjct: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360

Query: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE 420
           SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE
Sbjct: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE 420

Query: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
           SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS
Sbjct: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456

BLAST of Carg03639 vs. NCBI nr
Match: KAG6580736.1 (Aspartyl protease 25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 921.8 bits (2381), Expect = 2.3e-264
Identity = 454/456 (99.56%), Postives = 455/456 (99.78%), Query Frame = 0

Query: 1   MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
           MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI
Sbjct: 148 MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 207

Query: 61  KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
           KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC
Sbjct: 208 KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 267

Query: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
           SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 268 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 327

Query: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 328 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 387

Query: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
           KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF
Sbjct: 388 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 447

Query: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
           VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE
Sbjct: 448 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 507

Query: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE 420
           SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE
Sbjct: 508 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE 567

Query: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
           SGGGPSIILGAFQQQN YVE+DLVNQKIGFRQQTCS
Sbjct: 568 SGGGPSIILGAFQQQNLYVEFDLVNQKIGFRQQTCS 603

BLAST of Carg03639 vs. NCBI nr
Match: XP_022934749.1 (probable aspartyl protease At4g16563 [Cucurbita moschata])

HSP 1 Score: 920.2 bits (2377), Expect = 6.8e-264
Identity = 454/456 (99.56%), Postives = 454/456 (99.56%), Query Frame = 0

Query: 1   MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
           MAPPP LCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI
Sbjct: 1   MAPPPPLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60

Query: 61  KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
           KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC
Sbjct: 61  KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120

Query: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
           SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
           KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF
Sbjct: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300

Query: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
           VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE
Sbjct: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360

Query: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE 420
           SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWA PLSNYFALVSSSGVACLTVVTHKAAE
Sbjct: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWAPPLSNYFALVSSSGVACLTVVTHKAAE 420

Query: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
           SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS
Sbjct: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456

BLAST of Carg03639 vs. NCBI nr
Match: XP_023528159.1 (probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 915.6 bits (2365), Expect = 1.7e-262
Identity = 452/456 (99.12%), Postives = 453/456 (99.34%), Query Frame = 0

Query: 1   MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
           MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI
Sbjct: 1   MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60

Query: 61  KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
           KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC
Sbjct: 61  KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120

Query: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
           SFPKIDPA IPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPAGIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           VVQYGSGSTAGLLLSETLDF NKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFANKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
           KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF
Sbjct: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300

Query: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
           VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE
Sbjct: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360

Query: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE 420
           SLTGLRPCFDISKDKSVEFPELTF LKGGAKWALPLSNYFALVSSSGVACLTVVTHKAA+
Sbjct: 361 SLTGLRPCFDISKDKSVEFPELTFQLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAD 420

Query: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
           SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS
Sbjct: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456

BLAST of Carg03639 vs. NCBI nr
Match: XP_022982947.1 (probable aspartyl protease At4g16563 [Cucurbita maxima])

HSP 1 Score: 899.0 bits (2322), Expect = 1.6e-257
Identity = 441/456 (96.71%), Postives = 448/456 (98.25%), Query Frame = 0

Query: 1   MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
           MAPPP LCFFYILL+SSVSAIADTNPIT+PLSSFPHHSSSDPLQTLNFLASASQNRAHQI
Sbjct: 1   MAPPPPLCFFYILLVSSVSAIADTNPITIPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60

Query: 61  KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
           KAPKS+SNSVSKSPLSPHSYGAYSTPLSFGTP QTLHLIFDTGSSLVWLPCTSKYLCSEC
Sbjct: 61  KAPKSESNSVSKSPLSPHSYGAYSTPLSFGTPPQTLHLIFDTGSSLVWLPCTSKYLCSEC 120

Query: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
           SFPKIDPA IPRFIPKLSS+SKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPAGIPRFIPKLSSTSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           VVQYGSGSTAGLLLSETLDFP+KK TNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPDKKFTNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
           KFAYCLASRKFDDSPHAGELILDSSGAKTSGL+YTPFRQNPSVSNHAYKEYYYLTIRKIF
Sbjct: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKIF 300

Query: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
           VG KAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE
Sbjct: 301 VGKKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360

Query: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE 420
           SLTGLRPCFDISKDKSVEFPELTF LKGGAKW LPLSNYFALVSSSGVACLTVVTHK A+
Sbjct: 361 SLTGLRPCFDISKDKSVEFPELTFQLKGGAKWGLPLSNYFALVSSSGVACLTVVTHKTAD 420

Query: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
           SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS
Sbjct: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456

BLAST of Carg03639 vs. ExPASy Swiss-Prot
Match: Q940R4 (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 7.1e-46
Identity = 152/507 (29.98%), Postives = 234/507 (46.15%), Query Frame = 0

Query: 6   LLCFFYILLLS-----SVSAIADTNPITLPLS---SFPHHSSSDPLQTLNFLASASQNRA 65
           L+ F Y  +L      SVS++  + P+ L LS   S   HSSS PL  L   +S+S++ A
Sbjct: 5   LIFFLYTTILQYYFHFSVSSL--STPLLLHLSHSLSTSKHSSS-PLHLLK--SSSSRSSA 64

Query: 66  HQIKAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLC 125
              +    +       P+S  S   Y   LS G+ S  + L  DTGS LVW PC   + C
Sbjct: 65  RFRRHHHKQQQQQLSLPIS--SGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPC-RPFTC 124

Query: 126 SECSFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQ-CRSCN-----PKTE 185
             C    + P+        LSSS+  V C +P C+        S  C   N      +T 
Sbjct: 125 ILCESKPLPPSP----PSSLSSSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETG 184

Query: 186 NCTQT---CPAYVVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGR 245
           +C  +   CP +   YG GS    L S++L  P+  ++NF  GC+  ++ +P G+AGFGR
Sbjct: 185 DCNTSSYPCPPFYYAYGDGSLVAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGR 244

Query: 246 GSESLPSQMGL------KKFAYCLASRKFDDS--PHAGELIL------------------ 305
           G  SLP+Q+ +        F+YCL S  FD         LIL                  
Sbjct: 245 GRLSLPAQLAVHSPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHD 304

Query: 306 --DSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIFVGNKAVKVPYKYLVPGPDGN 365
             D    K +   +T   +NP    H Y  +Y ++++ I +G + +  P        +G 
Sbjct: 305 DGDDEKKKKNEFVFTEMLENP---KHPY--FYSVSLQGISIGKRNIPAPAMLRRIDKNGG 364

Query: 366 GGSIIDSGSTFTFMDKPVFEAVAQEIEKQLAN-RTRATDVESLTGLRPCFDISKDKSVEF 425
           GG ++DSG+TFT +    + +V +E + ++     RA  VE  +G+ PC+ +  +++V+ 
Sbjct: 365 GGVVVDSGTTFTMLPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYL--NQTVKV 424

Query: 426 PELTFHLKGG-AKWALPLSNYFALVSSSG--------VACLTVVT-HKAAESGGGPSIIL 457
           P L  H  G  +   LP  NYF      G        + CL ++     +E  GG   IL
Sbjct: 425 PALVLHFAGNRSSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAIL 484

BLAST of Carg03639 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 3.5e-37
Identity = 118/384 (30.73%), Postives = 173/384 (45.05%), Query Frame = 0

Query: 81  GAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSECSFPKIDPARIPRFIPKLSSS 140
           G Y T L  GTP++ ++++ DTGS +VWL C     C  C + + DP     F P+ S +
Sbjct: 140 GEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAP---CRRC-YSQSDPI----FDPRKSKT 199

Query: 141 SKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVVQYGSGS-TAGLLLSETLD 200
              + C +P C        +     CN + + C      Y V YG GS T G   +ETL 
Sbjct: 200 YATIPCSSPHCR-------RLDSAGCNTRRKTC-----LYQVSYGDGSFTVGDFSTETLT 259

Query: 201 FPNKKITNFVVGCSFLS---IHQPSGIAGFGRGSESLPSQMGLK---KFAYCLASRKFDD 260
           F   ++    +GC   +       +G+ G G+G  S P Q G +   KF+YCL  R    
Sbjct: 260 FRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASS 319

Query: 261 SPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIFVGNKAVK-VPYKY 320
            P +   ++  + A +    +TP   NP +       +YY+ +  I VG   V  V    
Sbjct: 320 KPSS---VVFGNAAVSRIARFTPLLSNPKLDT-----FYYVGLLGISVGGTRVPGVTASL 379

Query: 321 LVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVESLTGLRPCFDIS 380
                 GNGG IIDSG++ T + +P + A+            RA D         CFD+S
Sbjct: 380 FKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFD---TCFDLS 439

Query: 381 KDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAESGGGPSIILGAF 440
               V+ P +  H + GA  +LP +NY   V ++G  C        A + GG SII G  
Sbjct: 440 NMNEVKVPTVVLHFR-GADVSLPATNYLIPVDTNGKFCFAF-----AGTMGGLSII-GNI 485

Query: 441 QQQNFYVEYDLVNQKIGFRQQTCS 457
           QQQ F V YDL + ++GF    C+
Sbjct: 500 QQQGFRVVYDLASSRVGFAPGGCA 485

BLAST of Carg03639 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 1.1e-33
Identity = 125/385 (32.47%), Postives = 169/385 (43.90%), Query Frame = 0

Query: 81  GAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC-SFPKIDPARIPRFIPKLSS 140
           G Y   ++ GTP  +   I DTGS L+W  C     C++C S P       P F P+ SS
Sbjct: 94  GEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEP---CTQCFSQP------TPIFNPQDSS 153

Query: 141 SSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVVQYGSGSTA-GLLLSETL 200
           S   + C++  C      D+ S         E C      Y   YG GST  G + +ET 
Sbjct: 154 SFSTLPCESQYC-----QDLPS---------ETCNNNECQYTYGYGDGSTTQGYMATETF 213

Query: 201 DFPNKKITNFVVGC----SFLSIHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDS 260
            F    + N   GC            +G+ G G G  SLPSQ+G+ +F+YC+ S     S
Sbjct: 214 TFETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYG-SSS 273

Query: 261 PHAGELILDSSGAKTSGLTYTPFRQ--NPSVSNHAYKEYYYLTIRKIFVGNKAVKVPYKY 320
           P    L   +SG      + T      NP+        YYY+T++ I VG   + +P   
Sbjct: 274 PSTLALGSAASGVPEGSPSTTLIHSSLNPT--------YYYITLQGITVGGDNLGIPSST 333

Query: 321 LVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVESLTGLRPCFDIS 380
                DG GG IIDSG+T T++ +  + AVAQ    Q+      T  ES +GL  CF   
Sbjct: 334 FQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQI---NLPTVDESSSGLSTCFQQP 393

Query: 381 KDKS-VEFPELTFHLKGGAKWALPLSNYFALVS-SSGVACLTVVTHKAAESGGGPSIILG 440
            D S V+ PE++    GG    L L     L+S + GV CL +     + S  G S I G
Sbjct: 394 SDGSTVQVPEISMQFDGG---VLNLGEQNILISPAEGVICLAM----GSSSQLGIS-IFG 435

Query: 441 AFQQQNFYVEYDLVNQKIGFRQQTC 456
             QQQ   V YDL N  + F    C
Sbjct: 454 NIQQQETQVLYDLQNLAVSFVPTQC 435

BLAST of Carg03639 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 1.4e-33
Identity = 125/428 (29.21%), Postives = 183/428 (42.76%), Query Frame = 0

Query: 36  HHSSSDPLQTLNFLASASQNRAHQIKAPKSKSNSVSKSPLSPHS-YGAYSTPLSFGTPSQ 95
           H  S   L     L  A +  + +++  ++  N  S    S ++  G Y   LS GTP+Q
Sbjct: 47  HVDSGKNLTKFQLLERAIERGSRRLQRLEAMLNGPSGVETSVYAGDGEYLMNLSIGTPAQ 106

Query: 96  TLHLIFDTGSSLVWLPCTSKYLCSECSFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWI 155
               I DTGS L+W  C     C++C          P F P+ SSS   + C +  C  +
Sbjct: 107 PFSAIMDTGSDLIWTQCQP---CTQCF-----NQSTPIFNPQGSSSFSTLPCSSQLCQAL 166

Query: 156 FGPDVKSQCRSCNPKTENCTQTCPAYVVQYGSGS-TAGLLLSETLDFPNKKITNFVVGC- 215
             P               C+     Y   YG GS T G + +ETL F +  I N   GC 
Sbjct: 167 SSP--------------TCSNNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGCG 226

Query: 216 ---SFLSIHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSPHAGELILDSSGAKT 275
                      +G+ G GRG  SLPSQ+ + KF+YC+       S  +  L+   + + T
Sbjct: 227 ENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMT--PIGSSTPSNLLLGSLANSVT 286

Query: 276 SGLTYTPFRQNPSVSNHAYKEYYYLTIRKIFVGNKAVKV-PYKYLVPGPDGNGGSIIDSG 335
           +G   T   Q+  +       +YY+T+  + VG+  + + P  + +   +G GG IIDSG
Sbjct: 287 AGSPNTTLIQSSQIPT-----FYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSG 346

Query: 336 STFTFMDKPVFEAVAQEIEKQLANRTRATDVESLTGLRPCFDISKDKS-VEFPELTFHLK 395
           +T T+     +++V QE   Q+          S +G   CF    D S ++ P    H  
Sbjct: 347 TTLTYFVNNAYQSVRQEFISQI---NLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFD 406

Query: 396 GGAKWALPLSNYFALVSSSGVACLTVVTHKAAESGGGPSIILGAFQQQNFYVEYDLVNQK 455
           GG    LP  NYF +  S+G+ CL +       S  G S I G  QQQN  V YD  N  
Sbjct: 407 GG-DLELPSENYF-ISPSNGLICLAM-----GSSSQGMS-IFGNIQQQNMLVVYDTGNSV 434

BLAST of Carg03639 vs. ExPASy Swiss-Prot
Match: Q6F4N5 (Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.6e-32
Identity = 127/466 (27.25%), Postives = 205/466 (43.99%), Query Frame = 0

Query: 1   MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
           MA    +    +LL ++V+A A      L +    H SS  PL+++  LA     R   +
Sbjct: 1   MAATTTIPLLLLLLAATVAAAA----AELSVYHNVHPSSPSPLESIIALARDDDARLLFL 60

Query: 61  KAPKSKSNSVSKSPL-SPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSE 120
            + K+ +  VS +P+ S  +  +Y      G+PSQ L L  DT +   W  C+    C  
Sbjct: 61  SS-KAATAGVSSAPVASGQAPPSYVVRAGLGSPSQQLLLALDTSADATWAHCSP---CGT 120

Query: 121 CSFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPA 180
           C    +       F P  SSS   + C +  C    G    +     +      T    A
Sbjct: 121 CPSSSL-------FAPANSSSYASLPCSSSWCPLFQGQACPAPQGGGDAAPPPATLPTCA 180

Query: 181 YVVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPS------GIAGFGRGSESL 240
           +   +   S    L S+TL      I N+  GC   S+  P+      G+ G GRG  +L
Sbjct: 181 FSKPFADASFQAALASDTLRLGKDAIPNYTFGC-VSSVTGPTTNMPRQGLLGLGRGPMAL 240

Query: 241 PSQMGL---KKFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKE 300
            SQ G      F+YCL S  +     +G L L + G +   + YTP  +NP  S+     
Sbjct: 241 LSQAGSLYNGVFSYCLPS--YRSYYFSGSLRLGAGGGQPRSVRYTPMLRNPHRSS----- 300

Query: 301 YYYLTIRKIFVGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQL 360
            YY+ +  + VG+  VKVP            G+++DSG+  T    PV+ A+ +E  +Q+
Sbjct: 301 LYYVNVTGLSVGHAWVKVPAGSFAFDAATGAGTVVDSGTVITRWTAPVYAALREEFRRQV 360

Query: 361 ANRTRATDVESLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVAC 420
           A  +  T   SL     CF+  +  +   P +T H+ GG   ALP+ N     S++ +AC
Sbjct: 361 AAPSGYT---SLGAFDTCFNTDEVAAGGAPAVTVHMDGGVDLALPMENTLIHSSATPLAC 420

Query: 421 LTVVTHKAAESGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
           L +   +A ++      ++   QQQN  V +D+ N ++GF +++C+
Sbjct: 421 LAMA--EAPQNVNSVVNVIANLQQQNIRVVFDVANSRVGFAKESCN 438

BLAST of Carg03639 vs. ExPASy TrEMBL
Match: A0A6J1F3G5 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111441834 PE=3 SV=1)

HSP 1 Score: 920.2 bits (2377), Expect = 3.3e-264
Identity = 454/456 (99.56%), Postives = 454/456 (99.56%), Query Frame = 0

Query: 1   MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
           MAPPP LCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI
Sbjct: 1   MAPPPPLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60

Query: 61  KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
           KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC
Sbjct: 61  KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120

Query: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
           SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
           KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF
Sbjct: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300

Query: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
           VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE
Sbjct: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360

Query: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE 420
           SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWA PLSNYFALVSSSGVACLTVVTHKAAE
Sbjct: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWAPPLSNYFALVSSSGVACLTVVTHKAAE 420

Query: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
           SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS
Sbjct: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456

BLAST of Carg03639 vs. ExPASy TrEMBL
Match: A0A6J1IXY3 (probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111481640 PE=3 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 7.9e-258
Identity = 441/456 (96.71%), Postives = 448/456 (98.25%), Query Frame = 0

Query: 1   MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
           MAPPP LCFFYILL+SSVSAIADTNPIT+PLSSFPHHSSSDPLQTLNFLASASQNRAHQI
Sbjct: 1   MAPPPPLCFFYILLVSSVSAIADTNPITIPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60

Query: 61  KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
           KAPKS+SNSVSKSPLSPHSYGAYSTPLSFGTP QTLHLIFDTGSSLVWLPCTSKYLCSEC
Sbjct: 61  KAPKSESNSVSKSPLSPHSYGAYSTPLSFGTPPQTLHLIFDTGSSLVWLPCTSKYLCSEC 120

Query: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
           SFPKIDPA IPRFIPKLSS+SKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPAGIPRFIPKLSSTSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           VVQYGSGSTAGLLLSETLDFP+KK TNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPDKKFTNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
           KFAYCLASRKFDDSPHAGELILDSSGAKTSGL+YTPFRQNPSVSNHAYKEYYYLTIRKIF
Sbjct: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKIF 300

Query: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
           VG KAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE
Sbjct: 301 VGKKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360

Query: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE 420
           SLTGLRPCFDISKDKSVEFPELTF LKGGAKW LPLSNYFALVSSSGVACLTVVTHK A+
Sbjct: 361 SLTGLRPCFDISKDKSVEFPELTFQLKGGAKWGLPLSNYFALVSSSGVACLTVVTHKTAD 420

Query: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
           SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS
Sbjct: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456

BLAST of Carg03639 vs. ExPASy TrEMBL
Match: A0A5A7TRK2 (Aspartic proteinase nepenthesin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004410 PE=3 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.1e-232
Identity = 399/457 (87.31%), Postives = 424/457 (92.78%), Query Frame = 0

Query: 1   MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
           MA P  L FFYILL SS+SAI+++NPITLPL+S PH SSSDPLQ L FLASAS+NRAH+I
Sbjct: 1   MASPSPLSFFYILLFSSLSAISNSNPITLPLNSSPHLSSSDPLQALTFLASASKNRAHRI 60

Query: 61  KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
           K P  KSNSVSKSPLSPHSYGAYSTPLSFGTP QTLHLIFDTGSSLVW PCTS+YLC+EC
Sbjct: 61  KTP--KSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCTEC 120

Query: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
           SFPKIDP  IPRF+PKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           VVQYGSGSTAGLLLSETLDFPNKKI NFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPNKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
           KFAYCLASRKFDDS H+G+LILDSSG KTSGLTYT FRQNPSVSNHAYKEYYYL IRKI 
Sbjct: 241 KFAYCLASRKFDDSAHSGQLILDSSGVKTSGLTYTSFRQNPSVSNHAYKEYYYLNIRKII 300

Query: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
           VGN+AVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPV + VAQE EKQLANRTRATDVE
Sbjct: 301 VGNQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLDVVAQEFEKQLANRTRATDVE 360

Query: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE 420
           +LTGLRPCFD+SK+KSVEFPEL F  KGGAKWALPL+NYFALVSSSGVACLTVVTH   +
Sbjct: 361 TLTGLRPCFDVSKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHNTED 420

Query: 421 -SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
             GGGPS+ILGAFQQQNFYVEYDLVN+++GFR+QTC+
Sbjct: 421 GGGGGPSVILGAFQQQNFYVEYDLVNERLGFRKQTCT 455

BLAST of Carg03639 vs. ExPASy TrEMBL
Match: A0A1S3B6B5 (aspartic proteinase nepenthesin-2 OS=Cucumis melo OX=3656 GN=LOC103486666 PE=3 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.1e-232
Identity = 399/457 (87.31%), Postives = 424/457 (92.78%), Query Frame = 0

Query: 1   MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
           MA P  L FFYILL SS+SAI+++NPITLPL+S PH SSSDPLQ L FLASAS+NRAH+I
Sbjct: 1   MASPSPLSFFYILLFSSLSAISNSNPITLPLNSSPHLSSSDPLQALTFLASASKNRAHRI 60

Query: 61  KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
           K P  KSNSVSKSPLSPHSYGAYSTPLSFGTP QTLHLIFDTGSSLVW PCTS+YLC+EC
Sbjct: 61  KTP--KSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCTEC 120

Query: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
           SFPKIDP  IPRF+PKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           VVQYGSGSTAGLLLSETLDFPNKKI NFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPNKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
           KFAYCLASRKFDDS H+G+LILDSSG KTSGLTYT FRQNPSVSNHAYKEYYYL IRKI 
Sbjct: 241 KFAYCLASRKFDDSAHSGQLILDSSGVKTSGLTYTSFRQNPSVSNHAYKEYYYLNIRKII 300

Query: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
           VGN+AVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPV + VAQE EKQLANRTRATDVE
Sbjct: 301 VGNQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLDVVAQEFEKQLANRTRATDVE 360

Query: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE 420
           +LTGLRPCFD+SK+KSVEFPEL F  KGGAKWALPL+NYFALVSSSGVACLTVVTH   +
Sbjct: 361 TLTGLRPCFDVSKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHNTED 420

Query: 421 -SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
             GGGPS+ILGAFQQQNFYVEYDLVN+++GFR+QTC+
Sbjct: 421 GGGGGPSVILGAFQQQNFYVEYDLVNERLGFRKQTCT 455

BLAST of Carg03639 vs. ExPASy TrEMBL
Match: A0A6J1FJU4 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111446087 PE=3 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 2.0e-232
Identity = 403/459 (87.80%), Postives = 422/459 (91.94%), Query Frame = 0

Query: 1   MAPPPLLCFFYILLLSSVSAIAD--TNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAH 60
           MA PP LCF YILLL SVSAI D   N ITLPLS+ PH SSSDPLQ LNFLASASQNRAH
Sbjct: 1   MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSALPHPSSSDPLQNLNFLASASQNRAH 60

Query: 61  QIKAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCS 120
           QIK P  KSNSVSKSPLSPHSYGAYS PLSFGTP QTLHLIFDTGSSLVW PCTSKYLCS
Sbjct: 61  QIKTP--KSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCS 120

Query: 121 ECSFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCP 180
           +CSFPKIDP RIPRF+PKLSSSSKLVGCQNPKCAW+FGPDVKSQCR+CNPKTENCTQTCP
Sbjct: 121 QCSFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCP 180

Query: 181 AYVVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMG 240
           AY VQYGSGSTAGLLLSETLDFP++KI NFVVGCSFLSIHQPSGIAGFGRGSESLPSQMG
Sbjct: 181 AYAVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMG 240

Query: 241 LKKFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRK 300
           LKKFAYCLASRKFDDSPH+GELILDS GAKT  LTYTPFRQNPSVSNHAYKEYYYL+IRK
Sbjct: 241 LKKFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRK 300

Query: 301 IFVGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATD 360
           I VGN+ VKVPYKYLVPG DG+GGSIIDSGSTFTFMDKPVFEAVA+  EKQLANRTRATD
Sbjct: 301 ILVGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATD 360

Query: 361 VESLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKA 420
           VES TGLRPCFDISK+KSVEFPEL F  KGGAKWALPL+NYFALVSSSGVACLTVVTHK 
Sbjct: 361 VESATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKE 420

Query: 421 AESGG-GPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
           A  GG GPS+ILGAFQQQNFYVEYDLVN+++GFRQQ+CS
Sbjct: 421 AAGGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 457

BLAST of Carg03639 vs. TAIR 10
Match: AT3G52500.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 526.9 bits (1356), Expect = 1.6e-149
Identity = 270/470 (57.45%), Postives = 337/470 (71.70%), Query Frame = 0

Query: 9   FFYILLLSSVSAIADTNPITLPLSSFPH--HSSSDPLQTLNFLASASQNRAHQIK----- 68
           FF+++ LS VSA      + LPLS F H   S  DP  +L  LA +S  RAH++K     
Sbjct: 7   FFFLIFLSVVSA------VKLPLSPFSHSDQSPKDPYLSLRRLAESSIARAHKLKHGTSI 66

Query: 69  --------APKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTS 128
                   +  + S +V KSPLS  SYG YS  LSFGTPSQT+  +FDTGSSLVWLPCTS
Sbjct: 67  KPDEDALSSTTTASATVVKSPLSAKSYGGYSVSLSFGTPSQTIPFVFDTGSSLVWLPCTS 126

Query: 129 KYLCSECSFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENC 188
           +YLCS C F  +DP  IPRFIPK SSSSK++GCQ+PKC +++GP+V  QCR C+P T NC
Sbjct: 127 RYLCSGCDFSGLDPTLIPRFIPKNSSSSKIIGCQSPKCQFLYGPNV--QCRGCDPNTRNC 186

Query: 189 TQTCPAYVVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESL 248
           T  CP Y++QYG GSTAG+L++E LDFP+  + +FVVGCS +S  QP+GIAGFGRG  SL
Sbjct: 187 TVGCPPYILQYGLGSTAGVLITEKLDFPDLTVPDFVVGCSIISTRQPAGIAGFGRGPVSL 246

Query: 249 PSQMGLKKFAYCLASRKFDDSPHAGELILD-----SSGAKTSGLTYTPFRQNPSVSNHAY 308
           PSQM LK+F++CL SR+FDD+    +L LD     +SG+KT GLTYTPFR+NP+VSN A+
Sbjct: 247 PSQMNLKRFSHCLVSRRFDDTNVTTDLDLDTGSGHNSGSKTPGLTYTPFRKNPNVSNKAF 306

Query: 309 KEYYYLTIRKIFVGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEK 368
            EYYYL +R+I+VG K VK+PYKYL PG +G+GGSI+DSGSTFTFM++PVFE VA+E   
Sbjct: 307 LEYYYLNLRRIYVGRKHVKIPYKYLAPGTNGDGGSIVDSGSTFTFMERPVFELVAEEFAS 366

Query: 369 QLANRTRATDVESLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGV 428
           Q++N TR  D+E  TGL PCF+IS    V  PEL F  KGGAK  LPLSNYF  V ++  
Sbjct: 367 QMSNYTREKDLEKETGLGPCFNISGKGDVTVPELIFEFKGGAKLELPLSNYFTFVGNTDT 426

Query: 429 ACLTVVTHKAAE-SGG-GPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
            CLTVV+ K    SGG GP+IILG+FQQQN+ VEYDL N + GF ++ CS
Sbjct: 427 VCLTVVSDKTVNPSGGTGPAIILGSFQQQNYLVEYDLENDRFGFAKKKCS 468

BLAST of Carg03639 vs. TAIR 10
Match: AT4G16563.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 186.4 bits (472), Expect = 5.0e-47
Identity = 152/507 (29.98%), Postives = 234/507 (46.15%), Query Frame = 0

Query: 6   LLCFFYILLLS-----SVSAIADTNPITLPLS---SFPHHSSSDPLQTLNFLASASQNRA 65
           L+ F Y  +L      SVS++  + P+ L LS   S   HSSS PL  L   +S+S++ A
Sbjct: 5   LIFFLYTTILQYYFHFSVSSL--STPLLLHLSHSLSTSKHSSS-PLHLLK--SSSSRSSA 64

Query: 66  HQIKAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLC 125
              +    +       P+S  S   Y   LS G+ S  + L  DTGS LVW PC   + C
Sbjct: 65  RFRRHHHKQQQQQLSLPIS--SGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPC-RPFTC 124

Query: 126 SECSFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQ-CRSCN-----PKTE 185
             C    + P+        LSSS+  V C +P C+        S  C   N      +T 
Sbjct: 125 ILCESKPLPPSP----PSSLSSSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETG 184

Query: 186 NCTQT---CPAYVVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGR 245
           +C  +   CP +   YG GS    L S++L  P+  ++NF  GC+  ++ +P G+AGFGR
Sbjct: 185 DCNTSSYPCPPFYYAYGDGSLVAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGR 244

Query: 246 GSESLPSQMGL------KKFAYCLASRKFDDS--PHAGELIL------------------ 305
           G  SLP+Q+ +        F+YCL S  FD         LIL                  
Sbjct: 245 GRLSLPAQLAVHSPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHD 304

Query: 306 --DSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIFVGNKAVKVPYKYLVPGPDGN 365
             D    K +   +T   +NP    H Y  +Y ++++ I +G + +  P        +G 
Sbjct: 305 DGDDEKKKKNEFVFTEMLENP---KHPY--FYSVSLQGISIGKRNIPAPAMLRRIDKNGG 364

Query: 366 GGSIIDSGSTFTFMDKPVFEAVAQEIEKQLAN-RTRATDVESLTGLRPCFDISKDKSVEF 425
           GG ++DSG+TFT +    + +V +E + ++     RA  VE  +G+ PC+ +  +++V+ 
Sbjct: 365 GGVVVDSGTTFTMLPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYL--NQTVKV 424

Query: 426 PELTFHLKGG-AKWALPLSNYFALVSSSG--------VACLTVVT-HKAAESGGGPSIIL 457
           P L  H  G  +   LP  NYF      G        + CL ++     +E  GG   IL
Sbjct: 425 PALVLHFAGNRSSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAIL 484

BLAST of Carg03639 vs. TAIR 10
Match: AT5G45120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 184.9 bits (468), Expect = 1.5e-46
Identity = 147/488 (30.12%), Postives = 213/488 (43.65%), Query Frame = 0

Query: 6   LLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQIKAPKS 65
           L  F  I LL     +  TN         P  SSS      +FL       +  +  PKS
Sbjct: 8   LFLFLLITLL-----LNTTNKTQARQHKNPSSSSS------SFLVLTLTKSSVSLPTPKS 67

Query: 66  KSNSVSKSPLSP---------HSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTS-KY 125
           ++    K PLS               Y   L+ GTP Q + +  DTGS L W+PC +  +
Sbjct: 68  QTQERIKKPLSSVDVVMEPLREVRDGYLITLNIGTPPQAVQVYLDTGSDLTWVPCGNLSF 127

Query: 126 LCSECSFPKIDPARIPR-FIPKLSSSSKLVGCQNPKCAWI------FGPDVKSQCRSCNP 185
            C EC   K +  + P  F P  SS+S    C +  C  I      F P   + C     
Sbjct: 128 DCIECYDLKNNDLKSPSVFSPLHSSTSFRDSCASSFCVEIHSSDNPFDPCAVAGCSVSML 187

Query: 186 KTENCTQTCPAYVVQYGSGS-TAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFG 245
               C + CP++   YG G   +G+L  + L    + +  F  GC   +  +P GIAGFG
Sbjct: 188 LKSTCVRPCPSFAYTYGEGGLISGILTRDILKARTRDVPRFSFGCVTSTYREPIGIAGFG 247

Query: 246 RGSESLPSQMGL--KKFAYCLASRKFDDSPH-AGELILDSSGAK---TSGLTYTPFRQNP 305
           RG  SLPSQ+G   K F++C    KF ++P+ +  LIL +S      T  L +TP    P
Sbjct: 248 RGLLSLPSQLGFLEKGFSHCFLPFKFVNNPNISSPLILGASALSINLTDSLQFTPMLNTP 307

Query: 306 SVSNHAYKEYYYLTIRKIFVGNK--AVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVF 365
                 Y   YY+ +  I +G      +VP         GNGG ++DSG+T+T + +P +
Sbjct: 308 -----MYPNSYYIGLESITIGTNITPTQVPLTLRQFDSQGNGGMLVDSGTTYTHLPEPFY 367

Query: 366 EAVAQEIEKQLANRTRATDVESLTGLRPCF----------DISKDKSVEFPELTFHLKGG 425
             +   ++  +    RAT+ ES TG   C+           +  D  + FP +TFH    
Sbjct: 368 SQLLTTLQSTI-TYPRATETESRTGFDLCYKVPCPNNNLTSLENDVMMIFPSITFHFLNN 427

Query: 426 AKWALPLSNYFALVS--SSGVACLTVVTHKAAESGGGPSIILGAFQQQNFYVEYDLVNQK 456
           A   LP  N F  +S  S G     ++     +   GP+ + G+FQQQN  V YDL  ++
Sbjct: 428 ATLLLPQGNSFYAMSAPSDGSVVQCLLFQNMEDGDYGPAGVFGSFQQQNVKVVYDLEKER 478

BLAST of Carg03639 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 162.2 bits (409), Expect = 1.0e-39
Identity = 117/380 (30.79%), Postives = 174/380 (45.79%), Query Frame = 0

Query: 81  GAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSECSFPKIDPARIPRFIPKLSSS 140
           G Y T +  G P++ ++++ DTGS + WL CT    C++C + + +P     F P  SSS
Sbjct: 146 GEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTP---CADC-YHQTEPI----FEPSSSSS 205

Query: 141 SKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVVQYGSGS-TAGLLLSETLD 200
            + + C  P+C  +      S+CR+          TC  Y V YG GS T G   +ETL 
Sbjct: 206 YEPLSCDTPQCNAL----EVSECRNA---------TC-LYEVSYGDGSYTVGDFATETLT 265

Query: 201 FPNKKITNFVVGCSFLS---IHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSPH 260
             +  + N  VGC   +       +G+ G G G  +LPSQ+    F+YCL  R   DS  
Sbjct: 266 IGSTLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSFSYCLVDR---DS-- 325

Query: 261 AGELILDSSGAKTSGLTYTPFR-QNPSVSNHAYKEYYYLTIRKIFVGNKAVKVPYKYLVP 320
                 DS+     G + +P     P + NH    +YYL +  I VG + +++P      
Sbjct: 326 ------DSASTVDFGTSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEM 385

Query: 321 GPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVESLTGLRPCFDISKDK 380
              G+GG IIDSG+  T +   ++ ++     K   +  +A  V        C+++S   
Sbjct: 386 DESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFD---TCYNLSAKT 445

Query: 381 SVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAESGGGPSIILGAFQQQ 440
           +VE P + FH  GG   ALP  NY   V S G  CL      ++ +      I+G  QQQ
Sbjct: 446 TVEVPTVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLA------IIGNVQQQ 483

Query: 441 NFYVEYDLVNQKIGFRQQTC 456
              V +DL N  IGF    C
Sbjct: 506 GTRVTFDLANSLIGFSSNKC 483

BLAST of Carg03639 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 159.8 bits (403), Expect = 5.0e-39
Identity = 123/397 (30.98%), Postives = 178/397 (44.84%), Query Frame = 0

Query: 81  GAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSECSFPKIDPARIPRFIPKLSSS 140
           G Y   +  GTP +   LI DTGS L WL C   Y C   +    D        PK S+S
Sbjct: 158 GEYFMDVLVGTPPKHFSLILDTGSDLNWLQCLPCYDCFHQNGMFYD--------PKTSAS 217

Query: 141 SKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVVQYGSGS-TAGLLLSETLD 200
            K + C +P+C+ I  PD   QC S N       Q+CP Y   YG  S T G    ET  
Sbjct: 218 FKNITCNDPRCSLISSPDPPVQCESDN-------QSCP-YFYWYGDRSNTTGDFAVETFT 277

Query: 201 F---------PNKKITNFVVGCSFLS---IHQPSGIAGFGRGSESLPSQMGL---KKFAY 260
                        K+ N + GC   +       SG+ G GRG  S  SQ+       F+Y
Sbjct: 278 VNLTTTEGGSSEYKVGNMMFGCGHWNRGLFSGASGLLGLGRGPLSFSSQLQSLYGHSFSY 337

Query: 261 CLASRKFDDSPHAGELIL--DSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIFVG 320
           CL  R   ++  + +LI   D      + L +T F        ++ + +YY+ I+ I VG
Sbjct: 338 CLVDRN-SNTNVSSKLIFGEDKDLLNHTNLNFTSFVNG---KENSVETFYYIQIKSILVG 397

Query: 321 NKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEI-EKQLANRTRATDVES 380
            KA+ +P +      DG+GG+IIDSG+T ++  +P +E +  +  EK   N     D   
Sbjct: 398 GKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAEKMKENYPIFRDFPV 457

Query: 381 LTGLRPCFDIS--KDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAA 440
           L    PCF++S  ++ ++  PEL      G  W  P  N F  +S   V    + T K+ 
Sbjct: 458 LD---PCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLSEDLVCLAILGTPKST 517

Query: 441 ESGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
            S      I+G +QQQNF++ YD    ++GF    C+
Sbjct: 518 FS------IIGNYQQQNFHILYDTKRSRLGFTPTKCA 525

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7017489.11.6e-265100.00Aspartyl protease family protein 2, partial [Cucurbita argyrosperma subsp. argyr... [more]
KAG6580736.12.3e-26499.56Aspartyl protease 25, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022934749.16.8e-26499.56probable aspartyl protease At4g16563 [Cucurbita moschata][more]
XP_023528159.11.7e-26299.12probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo][more]
XP_022982947.11.6e-25796.71probable aspartyl protease At4g16563 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q940R47.1e-4629.98Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g1656... [more]
Q9LNJ33.5e-3730.73Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q766C21.1e-3332.47Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q766C31.4e-3329.21Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q6F4N52.6e-3227.25Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F3G53.3e-26499.56probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC1114418... [more]
A0A6J1IXY37.9e-25896.71probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111481640... [more]
A0A5A7TRK21.1e-23287.31Aspartic proteinase nepenthesin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3B6B51.1e-23287.31aspartic proteinase nepenthesin-2 OS=Cucumis melo OX=3656 GN=LOC103486666 PE=3 S... [more]
A0A6J1FJU42.0e-23287.80probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC1114460... [more]
Match NameE-valueIdentityDescription
AT3G52500.11.6e-14957.45Eukaryotic aspartyl protease family protein [more]
AT4G16563.15.0e-4729.98Eukaryotic aspartyl protease family protein [more]
AT5G45120.11.5e-4630.12Eukaryotic aspartyl protease family protein [more]
AT1G25510.11.0e-3930.79Eukaryotic aspartyl protease family protein [more]
AT2G42980.15.0e-3930.98Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 89..109
score: 50.41
coord: 323..334
score: 29.09
coord: 427..442
score: 30.89
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 63..260
e-value: 1.4E-33
score: 118.5
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 261..456
e-value: 4.3E-52
score: 178.5
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 77..455
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 83..250
e-value: 4.9E-29
score: 101.8
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 292..451
e-value: 5.7E-35
score: 120.5
NoneNo IPR availablePANTHERPTHR47967OS07G0603500 PROTEIN-RELATEDcoord: 8..455
NoneNo IPR availablePANTHERPTHR47967:SF36BNACNNG47670D PROTEINcoord: 8..455
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 98..109
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 83..451
score: 35.172466
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 82..455
e-value: 1.66654E-80
score: 248.332

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg03639-RACarg03639-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity