Homology
BLAST of Carg03639 vs. NCBI nr
Match:
KAG7017489.1 (Aspartyl protease family protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 925.6 bits (2391), Expect = 1.6e-265
Identity = 456/456 (100.00%), Postives = 456/456 (100.00%), Query Frame = 0
Query: 1 MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI
Sbjct: 1 MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
Query: 61 KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC
Sbjct: 61 KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
Query: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
Query: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
Query: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF
Sbjct: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
Query: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE
Sbjct: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
Query: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE 420
SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE
Sbjct: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE 420
Query: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS
Sbjct: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456
BLAST of Carg03639 vs. NCBI nr
Match:
KAG6580736.1 (Aspartyl protease 25, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 921.8 bits (2381), Expect = 2.3e-264
Identity = 454/456 (99.56%), Postives = 455/456 (99.78%), Query Frame = 0
Query: 1 MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI
Sbjct: 148 MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 207
Query: 61 KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC
Sbjct: 208 KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 267
Query: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 268 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 327
Query: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 328 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 387
Query: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF
Sbjct: 388 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 447
Query: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE
Sbjct: 448 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 507
Query: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE 420
SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE
Sbjct: 508 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE 567
Query: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
SGGGPSIILGAFQQQN YVE+DLVNQKIGFRQQTCS
Sbjct: 568 SGGGPSIILGAFQQQNLYVEFDLVNQKIGFRQQTCS 603
BLAST of Carg03639 vs. NCBI nr
Match:
XP_022934749.1 (probable aspartyl protease At4g16563 [Cucurbita moschata])
HSP 1 Score: 920.2 bits (2377), Expect = 6.8e-264
Identity = 454/456 (99.56%), Postives = 454/456 (99.56%), Query Frame = 0
Query: 1 MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
MAPPP LCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI
Sbjct: 1 MAPPPPLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
Query: 61 KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC
Sbjct: 61 KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
Query: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
Query: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
Query: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF
Sbjct: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
Query: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE
Sbjct: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
Query: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE 420
SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWA PLSNYFALVSSSGVACLTVVTHKAAE
Sbjct: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWAPPLSNYFALVSSSGVACLTVVTHKAAE 420
Query: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS
Sbjct: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456
BLAST of Carg03639 vs. NCBI nr
Match:
XP_023528159.1 (probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 915.6 bits (2365), Expect = 1.7e-262
Identity = 452/456 (99.12%), Postives = 453/456 (99.34%), Query Frame = 0
Query: 1 MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI
Sbjct: 1 MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
Query: 61 KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC
Sbjct: 61 KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
Query: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
SFPKIDPA IPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPAGIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
Query: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
VVQYGSGSTAGLLLSETLDF NKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFANKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
Query: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF
Sbjct: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
Query: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE
Sbjct: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
Query: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE 420
SLTGLRPCFDISKDKSVEFPELTF LKGGAKWALPLSNYFALVSSSGVACLTVVTHKAA+
Sbjct: 361 SLTGLRPCFDISKDKSVEFPELTFQLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAD 420
Query: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS
Sbjct: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456
BLAST of Carg03639 vs. NCBI nr
Match:
XP_022982947.1 (probable aspartyl protease At4g16563 [Cucurbita maxima])
HSP 1 Score: 899.0 bits (2322), Expect = 1.6e-257
Identity = 441/456 (96.71%), Postives = 448/456 (98.25%), Query Frame = 0
Query: 1 MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
MAPPP LCFFYILL+SSVSAIADTNPIT+PLSSFPHHSSSDPLQTLNFLASASQNRAHQI
Sbjct: 1 MAPPPPLCFFYILLVSSVSAIADTNPITIPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
Query: 61 KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
KAPKS+SNSVSKSPLSPHSYGAYSTPLSFGTP QTLHLIFDTGSSLVWLPCTSKYLCSEC
Sbjct: 61 KAPKSESNSVSKSPLSPHSYGAYSTPLSFGTPPQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
Query: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
SFPKIDPA IPRFIPKLSS+SKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPAGIPRFIPKLSSTSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
Query: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
VVQYGSGSTAGLLLSETLDFP+KK TNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPDKKFTNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
Query: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
KFAYCLASRKFDDSPHAGELILDSSGAKTSGL+YTPFRQNPSVSNHAYKEYYYLTIRKIF
Sbjct: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
Query: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
VG KAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE
Sbjct: 301 VGKKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
Query: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE 420
SLTGLRPCFDISKDKSVEFPELTF LKGGAKW LPLSNYFALVSSSGVACLTVVTHK A+
Sbjct: 361 SLTGLRPCFDISKDKSVEFPELTFQLKGGAKWGLPLSNYFALVSSSGVACLTVVTHKTAD 420
Query: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS
Sbjct: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456
BLAST of Carg03639 vs. ExPASy Swiss-Prot
Match:
Q940R4 (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g16563 PE=2 SV=1)
HSP 1 Score: 186.4 bits (472), Expect = 7.1e-46
Identity = 152/507 (29.98%), Postives = 234/507 (46.15%), Query Frame = 0
Query: 6 LLCFFYILLLS-----SVSAIADTNPITLPLS---SFPHHSSSDPLQTLNFLASASQNRA 65
L+ F Y +L SVS++ + P+ L LS S HSSS PL L +S+S++ A
Sbjct: 5 LIFFLYTTILQYYFHFSVSSL--STPLLLHLSHSLSTSKHSSS-PLHLLK--SSSSRSSA 64
Query: 66 HQIKAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLC 125
+ + P+S S Y LS G+ S + L DTGS LVW PC + C
Sbjct: 65 RFRRHHHKQQQQQLSLPIS--SGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPC-RPFTC 124
Query: 126 SECSFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQ-CRSCN-----PKTE 185
C + P+ LSSS+ V C +P C+ S C N +T
Sbjct: 125 ILCESKPLPPSP----PSSLSSSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETG 184
Query: 186 NCTQT---CPAYVVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGR 245
+C + CP + YG GS L S++L P+ ++NF GC+ ++ +P G+AGFGR
Sbjct: 185 DCNTSSYPCPPFYYAYGDGSLVAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGR 244
Query: 246 GSESLPSQMGL------KKFAYCLASRKFDDS--PHAGELIL------------------ 305
G SLP+Q+ + F+YCL S FD LIL
Sbjct: 245 GRLSLPAQLAVHSPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHD 304
Query: 306 --DSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIFVGNKAVKVPYKYLVPGPDGN 365
D K + +T +NP H Y +Y ++++ I +G + + P +G
Sbjct: 305 DGDDEKKKKNEFVFTEMLENP---KHPY--FYSVSLQGISIGKRNIPAPAMLRRIDKNGG 364
Query: 366 GGSIIDSGSTFTFMDKPVFEAVAQEIEKQLAN-RTRATDVESLTGLRPCFDISKDKSVEF 425
GG ++DSG+TFT + + +V +E + ++ RA VE +G+ PC+ + +++V+
Sbjct: 365 GGVVVDSGTTFTMLPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYL--NQTVKV 424
Query: 426 PELTFHLKGG-AKWALPLSNYFALVSSSG--------VACLTVVT-HKAAESGGGPSIIL 457
P L H G + LP NYF G + CL ++ +E GG IL
Sbjct: 425 PALVLHFAGNRSSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAIL 484
BLAST of Carg03639 vs. ExPASy Swiss-Prot
Match:
Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)
HSP 1 Score: 157.5 bits (397), Expect = 3.5e-37
Identity = 118/384 (30.73%), Postives = 173/384 (45.05%), Query Frame = 0
Query: 81 GAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSECSFPKIDPARIPRFIPKLSSS 140
G Y T L GTP++ ++++ DTGS +VWL C C C + + DP F P+ S +
Sbjct: 140 GEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAP---CRRC-YSQSDPI----FDPRKSKT 199
Query: 141 SKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVVQYGSGS-TAGLLLSETLD 200
+ C +P C + CN + + C Y V YG GS T G +ETL
Sbjct: 200 YATIPCSSPHCR-------RLDSAGCNTRRKTC-----LYQVSYGDGSFTVGDFSTETLT 259
Query: 201 FPNKKITNFVVGCSFLS---IHQPSGIAGFGRGSESLPSQMGLK---KFAYCLASRKFDD 260
F ++ +GC + +G+ G G+G S P Q G + KF+YCL R
Sbjct: 260 FRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASS 319
Query: 261 SPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIFVGNKAVK-VPYKY 320
P + ++ + A + +TP NP + +YY+ + I VG V V
Sbjct: 320 KPSS---VVFGNAAVSRIARFTPLLSNPKLDT-----FYYVGLLGISVGGTRVPGVTASL 379
Query: 321 LVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVESLTGLRPCFDIS 380
GNGG IIDSG++ T + +P + A+ RA D CFD+S
Sbjct: 380 FKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFD---TCFDLS 439
Query: 381 KDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAESGGGPSIILGAF 440
V+ P + H + GA +LP +NY V ++G C A + GG SII G
Sbjct: 440 NMNEVKVPTVVLHFR-GADVSLPATNYLIPVDTNGKFCFAF-----AGTMGGLSII-GNI 485
Query: 441 QQQNFYVEYDLVNQKIGFRQQTCS 457
QQQ F V YDL + ++GF C+
Sbjct: 500 QQQGFRVVYDLASSRVGFAPGGCA 485
BLAST of Carg03639 vs. ExPASy Swiss-Prot
Match:
Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)
HSP 1 Score: 146.0 bits (367), Expect = 1.1e-33
Identity = 125/385 (32.47%), Postives = 169/385 (43.90%), Query Frame = 0
Query: 81 GAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC-SFPKIDPARIPRFIPKLSS 140
G Y ++ GTP + I DTGS L+W C C++C S P P F P+ SS
Sbjct: 94 GEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEP---CTQCFSQP------TPIFNPQDSS 153
Query: 141 SSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVVQYGSGSTA-GLLLSETL 200
S + C++ C D+ S E C Y YG GST G + +ET
Sbjct: 154 SFSTLPCESQYC-----QDLPS---------ETCNNNECQYTYGYGDGSTTQGYMATETF 213
Query: 201 DFPNKKITNFVVGC----SFLSIHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDS 260
F + N GC +G+ G G G SLPSQ+G+ +F+YC+ S S
Sbjct: 214 TFETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYG-SSS 273
Query: 261 PHAGELILDSSGAKTSGLTYTPFRQ--NPSVSNHAYKEYYYLTIRKIFVGNKAVKVPYKY 320
P L +SG + T NP+ YYY+T++ I VG + +P
Sbjct: 274 PSTLALGSAASGVPEGSPSTTLIHSSLNPT--------YYYITLQGITVGGDNLGIPSST 333
Query: 321 LVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVESLTGLRPCFDIS 380
DG GG IIDSG+T T++ + + AVAQ Q+ T ES +GL CF
Sbjct: 334 FQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQI---NLPTVDESSSGLSTCFQQP 393
Query: 381 KDKS-VEFPELTFHLKGGAKWALPLSNYFALVS-SSGVACLTVVTHKAAESGGGPSIILG 440
D S V+ PE++ GG L L L+S + GV CL + + S G S I G
Sbjct: 394 SDGSTVQVPEISMQFDGG---VLNLGEQNILISPAEGVICLAM----GSSSQLGIS-IFG 435
Query: 441 AFQQQNFYVEYDLVNQKIGFRQQTC 456
QQQ V YDL N + F C
Sbjct: 454 NIQQQETQVLYDLQNLAVSFVPTQC 435
BLAST of Carg03639 vs. ExPASy Swiss-Prot
Match:
Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)
HSP 1 Score: 145.6 bits (366), Expect = 1.4e-33
Identity = 125/428 (29.21%), Postives = 183/428 (42.76%), Query Frame = 0
Query: 36 HHSSSDPLQTLNFLASASQNRAHQIKAPKSKSNSVSKSPLSPHS-YGAYSTPLSFGTPSQ 95
H S L L A + + +++ ++ N S S ++ G Y LS GTP+Q
Sbjct: 47 HVDSGKNLTKFQLLERAIERGSRRLQRLEAMLNGPSGVETSVYAGDGEYLMNLSIGTPAQ 106
Query: 96 TLHLIFDTGSSLVWLPCTSKYLCSECSFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWI 155
I DTGS L+W C C++C P F P+ SSS + C + C +
Sbjct: 107 PFSAIMDTGSDLIWTQCQP---CTQCF-----NQSTPIFNPQGSSSFSTLPCSSQLCQAL 166
Query: 156 FGPDVKSQCRSCNPKTENCTQTCPAYVVQYGSGS-TAGLLLSETLDFPNKKITNFVVGC- 215
P C+ Y YG GS T G + +ETL F + I N GC
Sbjct: 167 SSP--------------TCSNNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGCG 226
Query: 216 ---SFLSIHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSPHAGELILDSSGAKT 275
+G+ G GRG SLPSQ+ + KF+YC+ S + L+ + + T
Sbjct: 227 ENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMT--PIGSSTPSNLLLGSLANSVT 286
Query: 276 SGLTYTPFRQNPSVSNHAYKEYYYLTIRKIFVGNKAVKV-PYKYLVPGPDGNGGSIIDSG 335
+G T Q+ + +YY+T+ + VG+ + + P + + +G GG IIDSG
Sbjct: 287 AGSPNTTLIQSSQIPT-----FYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSG 346
Query: 336 STFTFMDKPVFEAVAQEIEKQLANRTRATDVESLTGLRPCFDISKDKS-VEFPELTFHLK 395
+T T+ +++V QE Q+ S +G CF D S ++ P H
Sbjct: 347 TTLTYFVNNAYQSVRQEFISQI---NLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFD 406
Query: 396 GGAKWALPLSNYFALVSSSGVACLTVVTHKAAESGGGPSIILGAFQQQNFYVEYDLVNQK 455
GG LP NYF + S+G+ CL + S G S I G QQQN V YD N
Sbjct: 407 GG-DLELPSENYF-ISPSNGLICLAM-----GSSSQGMS-IFGNIQQQNMLVVYDTGNSV 434
BLAST of Carg03639 vs. ExPASy Swiss-Prot
Match:
Q6F4N5 (Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1)
HSP 1 Score: 141.4 bits (355), Expect = 2.6e-32
Identity = 127/466 (27.25%), Postives = 205/466 (43.99%), Query Frame = 0
Query: 1 MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
MA + +LL ++V+A A L + H SS PL+++ LA R +
Sbjct: 1 MAATTTIPLLLLLLAATVAAAA----AELSVYHNVHPSSPSPLESIIALARDDDARLLFL 60
Query: 61 KAPKSKSNSVSKSPL-SPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSE 120
+ K+ + VS +P+ S + +Y G+PSQ L L DT + W C+ C
Sbjct: 61 SS-KAATAGVSSAPVASGQAPPSYVVRAGLGSPSQQLLLALDTSADATWAHCSP---CGT 120
Query: 121 CSFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPA 180
C + F P SSS + C + C G + + T A
Sbjct: 121 CPSSSL-------FAPANSSSYASLPCSSSWCPLFQGQACPAPQGGGDAAPPPATLPTCA 180
Query: 181 YVVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPS------GIAGFGRGSESL 240
+ + S L S+TL I N+ GC S+ P+ G+ G GRG +L
Sbjct: 181 FSKPFADASFQAALASDTLRLGKDAIPNYTFGC-VSSVTGPTTNMPRQGLLGLGRGPMAL 240
Query: 241 PSQMGL---KKFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKE 300
SQ G F+YCL S + +G L L + G + + YTP +NP S+
Sbjct: 241 LSQAGSLYNGVFSYCLPS--YRSYYFSGSLRLGAGGGQPRSVRYTPMLRNPHRSS----- 300
Query: 301 YYYLTIRKIFVGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQL 360
YY+ + + VG+ VKVP G+++DSG+ T PV+ A+ +E +Q+
Sbjct: 301 LYYVNVTGLSVGHAWVKVPAGSFAFDAATGAGTVVDSGTVITRWTAPVYAALREEFRRQV 360
Query: 361 ANRTRATDVESLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVAC 420
A + T SL CF+ + + P +T H+ GG ALP+ N S++ +AC
Sbjct: 361 AAPSGYT---SLGAFDTCFNTDEVAAGGAPAVTVHMDGGVDLALPMENTLIHSSATPLAC 420
Query: 421 LTVVTHKAAESGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
L + +A ++ ++ QQQN V +D+ N ++GF +++C+
Sbjct: 421 LAMA--EAPQNVNSVVNVIANLQQQNIRVVFDVANSRVGFAKESCN 438
BLAST of Carg03639 vs. ExPASy TrEMBL
Match:
A0A6J1F3G5 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111441834 PE=3 SV=1)
HSP 1 Score: 920.2 bits (2377), Expect = 3.3e-264
Identity = 454/456 (99.56%), Postives = 454/456 (99.56%), Query Frame = 0
Query: 1 MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
MAPPP LCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI
Sbjct: 1 MAPPPPLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
Query: 61 KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC
Sbjct: 61 KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
Query: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
Query: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
Query: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF
Sbjct: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
Query: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE
Sbjct: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
Query: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE 420
SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWA PLSNYFALVSSSGVACLTVVTHKAAE
Sbjct: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWAPPLSNYFALVSSSGVACLTVVTHKAAE 420
Query: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS
Sbjct: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456
BLAST of Carg03639 vs. ExPASy TrEMBL
Match:
A0A6J1IXY3 (probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111481640 PE=3 SV=1)
HSP 1 Score: 899.0 bits (2322), Expect = 7.9e-258
Identity = 441/456 (96.71%), Postives = 448/456 (98.25%), Query Frame = 0
Query: 1 MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
MAPPP LCFFYILL+SSVSAIADTNPIT+PLSSFPHHSSSDPLQTLNFLASASQNRAHQI
Sbjct: 1 MAPPPPLCFFYILLVSSVSAIADTNPITIPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
Query: 61 KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
KAPKS+SNSVSKSPLSPHSYGAYSTPLSFGTP QTLHLIFDTGSSLVWLPCTSKYLCSEC
Sbjct: 61 KAPKSESNSVSKSPLSPHSYGAYSTPLSFGTPPQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
Query: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
SFPKIDPA IPRFIPKLSS+SKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPAGIPRFIPKLSSTSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
Query: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
VVQYGSGSTAGLLLSETLDFP+KK TNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPDKKFTNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
Query: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
KFAYCLASRKFDDSPHAGELILDSSGAKTSGL+YTPFRQNPSVSNHAYKEYYYLTIRKIF
Sbjct: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
Query: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
VG KAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE
Sbjct: 301 VGKKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
Query: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE 420
SLTGLRPCFDISKDKSVEFPELTF LKGGAKW LPLSNYFALVSSSGVACLTVVTHK A+
Sbjct: 361 SLTGLRPCFDISKDKSVEFPELTFQLKGGAKWGLPLSNYFALVSSSGVACLTVVTHKTAD 420
Query: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS
Sbjct: 421 SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456
BLAST of Carg03639 vs. ExPASy TrEMBL
Match:
A0A5A7TRK2 (Aspartic proteinase nepenthesin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004410 PE=3 SV=1)
HSP 1 Score: 815.5 bits (2105), Expect = 1.1e-232
Identity = 399/457 (87.31%), Postives = 424/457 (92.78%), Query Frame = 0
Query: 1 MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
MA P L FFYILL SS+SAI+++NPITLPL+S PH SSSDPLQ L FLASAS+NRAH+I
Sbjct: 1 MASPSPLSFFYILLFSSLSAISNSNPITLPLNSSPHLSSSDPLQALTFLASASKNRAHRI 60
Query: 61 KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
K P KSNSVSKSPLSPHSYGAYSTPLSFGTP QTLHLIFDTGSSLVW PCTS+YLC+EC
Sbjct: 61 KTP--KSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCTEC 120
Query: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
SFPKIDP IPRF+PKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
Query: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
VVQYGSGSTAGLLLSETLDFPNKKI NFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPNKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
Query: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
KFAYCLASRKFDDS H+G+LILDSSG KTSGLTYT FRQNPSVSNHAYKEYYYL IRKI
Sbjct: 241 KFAYCLASRKFDDSAHSGQLILDSSGVKTSGLTYTSFRQNPSVSNHAYKEYYYLNIRKII 300
Query: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
VGN+AVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPV + VAQE EKQLANRTRATDVE
Sbjct: 301 VGNQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLDVVAQEFEKQLANRTRATDVE 360
Query: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE 420
+LTGLRPCFD+SK+KSVEFPEL F KGGAKWALPL+NYFALVSSSGVACLTVVTH +
Sbjct: 361 TLTGLRPCFDVSKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHNTED 420
Query: 421 -SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
GGGPS+ILGAFQQQNFYVEYDLVN+++GFR+QTC+
Sbjct: 421 GGGGGPSVILGAFQQQNFYVEYDLVNERLGFRKQTCT 455
BLAST of Carg03639 vs. ExPASy TrEMBL
Match:
A0A1S3B6B5 (aspartic proteinase nepenthesin-2 OS=Cucumis melo OX=3656 GN=LOC103486666 PE=3 SV=1)
HSP 1 Score: 815.5 bits (2105), Expect = 1.1e-232
Identity = 399/457 (87.31%), Postives = 424/457 (92.78%), Query Frame = 0
Query: 1 MAPPPLLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQI 60
MA P L FFYILL SS+SAI+++NPITLPL+S PH SSSDPLQ L FLASAS+NRAH+I
Sbjct: 1 MASPSPLSFFYILLFSSLSAISNSNPITLPLNSSPHLSSSDPLQALTFLASASKNRAHRI 60
Query: 61 KAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSEC 120
K P KSNSVSKSPLSPHSYGAYSTPLSFGTP QTLHLIFDTGSSLVW PCTS+YLC+EC
Sbjct: 61 KTP--KSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCTEC 120
Query: 121 SFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
SFPKIDP IPRF+PKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180
Query: 181 VVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
VVQYGSGSTAGLLLSETLDFPNKKI NFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPNKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
Query: 241 KFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIF 300
KFAYCLASRKFDDS H+G+LILDSSG KTSGLTYT FRQNPSVSNHAYKEYYYL IRKI
Sbjct: 241 KFAYCLASRKFDDSAHSGQLILDSSGVKTSGLTYTSFRQNPSVSNHAYKEYYYLNIRKII 300
Query: 301 VGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVE 360
VGN+AVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPV + VAQE EKQLANRTRATDVE
Sbjct: 301 VGNQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLDVVAQEFEKQLANRTRATDVE 360
Query: 361 SLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAE 420
+LTGLRPCFD+SK+KSVEFPEL F KGGAKWALPL+NYFALVSSSGVACLTVVTH +
Sbjct: 361 TLTGLRPCFDVSKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHNTED 420
Query: 421 -SGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
GGGPS+ILGAFQQQNFYVEYDLVN+++GFR+QTC+
Sbjct: 421 GGGGGPSVILGAFQQQNFYVEYDLVNERLGFRKQTCT 455
BLAST of Carg03639 vs. ExPASy TrEMBL
Match:
A0A6J1FJU4 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111446087 PE=3 SV=1)
HSP 1 Score: 814.7 bits (2103), Expect = 2.0e-232
Identity = 403/459 (87.80%), Postives = 422/459 (91.94%), Query Frame = 0
Query: 1 MAPPPLLCFFYILLLSSVSAIAD--TNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAH 60
MA PP LCF YILLL SVSAI D N ITLPLS+ PH SSSDPLQ LNFLASASQNRAH
Sbjct: 1 MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSALPHPSSSDPLQNLNFLASASQNRAH 60
Query: 61 QIKAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCS 120
QIK P KSNSVSKSPLSPHSYGAYS PLSFGTP QTLHLIFDTGSSLVW PCTSKYLCS
Sbjct: 61 QIKTP--KSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCS 120
Query: 121 ECSFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCP 180
+CSFPKIDP RIPRF+PKLSSSSKLVGCQNPKCAW+FGPDVKSQCR+CNPKTENCTQTCP
Sbjct: 121 QCSFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCP 180
Query: 181 AYVVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMG 240
AY VQYGSGSTAGLLLSETLDFP++KI NFVVGCSFLSIHQPSGIAGFGRGSESLPSQMG
Sbjct: 181 AYAVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMG 240
Query: 241 LKKFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRK 300
LKKFAYCLASRKFDDSPH+GELILDS GAKT LTYTPFRQNPSVSNHAYKEYYYL+IRK
Sbjct: 241 LKKFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRK 300
Query: 301 IFVGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATD 360
I VGN+ VKVPYKYLVPG DG+GGSIIDSGSTFTFMDKPVFEAVA+ EKQLANRTRATD
Sbjct: 301 ILVGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATD 360
Query: 361 VESLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKA 420
VES TGLRPCFDISK+KSVEFPEL F KGGAKWALPL+NYFALVSSSGVACLTVVTHK
Sbjct: 361 VESATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKE 420
Query: 421 AESGG-GPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
A GG GPS+ILGAFQQQNFYVEYDLVN+++GFRQQ+CS
Sbjct: 421 AAGGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 457
BLAST of Carg03639 vs. TAIR 10
Match:
AT3G52500.1 (Eukaryotic aspartyl protease family protein )
HSP 1 Score: 526.9 bits (1356), Expect = 1.6e-149
Identity = 270/470 (57.45%), Postives = 337/470 (71.70%), Query Frame = 0
Query: 9 FFYILLLSSVSAIADTNPITLPLSSFPH--HSSSDPLQTLNFLASASQNRAHQIK----- 68
FF+++ LS VSA + LPLS F H S DP +L LA +S RAH++K
Sbjct: 7 FFFLIFLSVVSA------VKLPLSPFSHSDQSPKDPYLSLRRLAESSIARAHKLKHGTSI 66
Query: 69 --------APKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTS 128
+ + S +V KSPLS SYG YS LSFGTPSQT+ +FDTGSSLVWLPCTS
Sbjct: 67 KPDEDALSSTTTASATVVKSPLSAKSYGGYSVSLSFGTPSQTIPFVFDTGSSLVWLPCTS 126
Query: 129 KYLCSECSFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENC 188
+YLCS C F +DP IPRFIPK SSSSK++GCQ+PKC +++GP+V QCR C+P T NC
Sbjct: 127 RYLCSGCDFSGLDPTLIPRFIPKNSSSSKIIGCQSPKCQFLYGPNV--QCRGCDPNTRNC 186
Query: 189 TQTCPAYVVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESL 248
T CP Y++QYG GSTAG+L++E LDFP+ + +FVVGCS +S QP+GIAGFGRG SL
Sbjct: 187 TVGCPPYILQYGLGSTAGVLITEKLDFPDLTVPDFVVGCSIISTRQPAGIAGFGRGPVSL 246
Query: 249 PSQMGLKKFAYCLASRKFDDSPHAGELILD-----SSGAKTSGLTYTPFRQNPSVSNHAY 308
PSQM LK+F++CL SR+FDD+ +L LD +SG+KT GLTYTPFR+NP+VSN A+
Sbjct: 247 PSQMNLKRFSHCLVSRRFDDTNVTTDLDLDTGSGHNSGSKTPGLTYTPFRKNPNVSNKAF 306
Query: 309 KEYYYLTIRKIFVGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEK 368
EYYYL +R+I+VG K VK+PYKYL PG +G+GGSI+DSGSTFTFM++PVFE VA+E
Sbjct: 307 LEYYYLNLRRIYVGRKHVKIPYKYLAPGTNGDGGSIVDSGSTFTFMERPVFELVAEEFAS 366
Query: 369 QLANRTRATDVESLTGLRPCFDISKDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGV 428
Q++N TR D+E TGL PCF+IS V PEL F KGGAK LPLSNYF V ++
Sbjct: 367 QMSNYTREKDLEKETGLGPCFNISGKGDVTVPELIFEFKGGAKLELPLSNYFTFVGNTDT 426
Query: 429 ACLTVVTHKAAE-SGG-GPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
CLTVV+ K SGG GP+IILG+FQQQN+ VEYDL N + GF ++ CS
Sbjct: 427 VCLTVVSDKTVNPSGGTGPAIILGSFQQQNYLVEYDLENDRFGFAKKKCS 468
BLAST of Carg03639 vs. TAIR 10
Match:
AT4G16563.1 (Eukaryotic aspartyl protease family protein )
HSP 1 Score: 186.4 bits (472), Expect = 5.0e-47
Identity = 152/507 (29.98%), Postives = 234/507 (46.15%), Query Frame = 0
Query: 6 LLCFFYILLLS-----SVSAIADTNPITLPLS---SFPHHSSSDPLQTLNFLASASQNRA 65
L+ F Y +L SVS++ + P+ L LS S HSSS PL L +S+S++ A
Sbjct: 5 LIFFLYTTILQYYFHFSVSSL--STPLLLHLSHSLSTSKHSSS-PLHLLK--SSSSRSSA 64
Query: 66 HQIKAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLC 125
+ + P+S S Y LS G+ S + L DTGS LVW PC + C
Sbjct: 65 RFRRHHHKQQQQQLSLPIS--SGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPC-RPFTC 124
Query: 126 SECSFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQ-CRSCN-----PKTE 185
C + P+ LSSS+ V C +P C+ S C N +T
Sbjct: 125 ILCESKPLPPSP----PSSLSSSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETG 184
Query: 186 NCTQT---CPAYVVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGR 245
+C + CP + YG GS L S++L P+ ++NF GC+ ++ +P G+AGFGR
Sbjct: 185 DCNTSSYPCPPFYYAYGDGSLVAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGR 244
Query: 246 GSESLPSQMGL------KKFAYCLASRKFDDS--PHAGELIL------------------ 305
G SLP+Q+ + F+YCL S FD LIL
Sbjct: 245 GRLSLPAQLAVHSPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHD 304
Query: 306 --DSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIFVGNKAVKVPYKYLVPGPDGN 365
D K + +T +NP H Y +Y ++++ I +G + + P +G
Sbjct: 305 DGDDEKKKKNEFVFTEMLENP---KHPY--FYSVSLQGISIGKRNIPAPAMLRRIDKNGG 364
Query: 366 GGSIIDSGSTFTFMDKPVFEAVAQEIEKQLAN-RTRATDVESLTGLRPCFDISKDKSVEF 425
GG ++DSG+TFT + + +V +E + ++ RA VE +G+ PC+ + +++V+
Sbjct: 365 GGVVVDSGTTFTMLPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYL--NQTVKV 424
Query: 426 PELTFHLKGG-AKWALPLSNYFALVSSSG--------VACLTVVT-HKAAESGGGPSIIL 457
P L H G + LP NYF G + CL ++ +E GG IL
Sbjct: 425 PALVLHFAGNRSSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAIL 484
BLAST of Carg03639 vs. TAIR 10
Match:
AT5G45120.1 (Eukaryotic aspartyl protease family protein )
HSP 1 Score: 184.9 bits (468), Expect = 1.5e-46
Identity = 147/488 (30.12%), Postives = 213/488 (43.65%), Query Frame = 0
Query: 6 LLCFFYILLLSSVSAIADTNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAHQIKAPKS 65
L F I LL + TN P SSS +FL + + PKS
Sbjct: 8 LFLFLLITLL-----LNTTNKTQARQHKNPSSSSS------SFLVLTLTKSSVSLPTPKS 67
Query: 66 KSNSVSKSPLSP---------HSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTS-KY 125
++ K PLS Y L+ GTP Q + + DTGS L W+PC + +
Sbjct: 68 QTQERIKKPLSSVDVVMEPLREVRDGYLITLNIGTPPQAVQVYLDTGSDLTWVPCGNLSF 127
Query: 126 LCSECSFPKIDPARIPR-FIPKLSSSSKLVGCQNPKCAWI------FGPDVKSQCRSCNP 185
C EC K + + P F P SS+S C + C I F P + C
Sbjct: 128 DCIECYDLKNNDLKSPSVFSPLHSSTSFRDSCASSFCVEIHSSDNPFDPCAVAGCSVSML 187
Query: 186 KTENCTQTCPAYVVQYGSGS-TAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFG 245
C + CP++ YG G +G+L + L + + F GC + +P GIAGFG
Sbjct: 188 LKSTCVRPCPSFAYTYGEGGLISGILTRDILKARTRDVPRFSFGCVTSTYREPIGIAGFG 247
Query: 246 RGSESLPSQMGL--KKFAYCLASRKFDDSPH-AGELILDSSGAK---TSGLTYTPFRQNP 305
RG SLPSQ+G K F++C KF ++P+ + LIL +S T L +TP P
Sbjct: 248 RGLLSLPSQLGFLEKGFSHCFLPFKFVNNPNISSPLILGASALSINLTDSLQFTPMLNTP 307
Query: 306 SVSNHAYKEYYYLTIRKIFVGNK--AVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVF 365
Y YY+ + I +G +VP GNGG ++DSG+T+T + +P +
Sbjct: 308 -----MYPNSYYIGLESITIGTNITPTQVPLTLRQFDSQGNGGMLVDSGTTYTHLPEPFY 367
Query: 366 EAVAQEIEKQLANRTRATDVESLTGLRPCF----------DISKDKSVEFPELTFHLKGG 425
+ ++ + RAT+ ES TG C+ + D + FP +TFH
Sbjct: 368 SQLLTTLQSTI-TYPRATETESRTGFDLCYKVPCPNNNLTSLENDVMMIFPSITFHFLNN 427
Query: 426 AKWALPLSNYFALVS--SSGVACLTVVTHKAAESGGGPSIILGAFQQQNFYVEYDLVNQK 456
A LP N F +S S G ++ + GP+ + G+FQQQN V YDL ++
Sbjct: 428 ATLLLPQGNSFYAMSAPSDGSVVQCLLFQNMEDGDYGPAGVFGSFQQQNVKVVYDLEKER 478
BLAST of Carg03639 vs. TAIR 10
Match:
AT1G25510.1 (Eukaryotic aspartyl protease family protein )
HSP 1 Score: 162.2 bits (409), Expect = 1.0e-39
Identity = 117/380 (30.79%), Postives = 174/380 (45.79%), Query Frame = 0
Query: 81 GAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSECSFPKIDPARIPRFIPKLSSS 140
G Y T + G P++ ++++ DTGS + WL CT C++C + + +P F P SSS
Sbjct: 146 GEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTP---CADC-YHQTEPI----FEPSSSSS 205
Query: 141 SKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVVQYGSGS-TAGLLLSETLD 200
+ + C P+C + S+CR+ TC Y V YG GS T G +ETL
Sbjct: 206 YEPLSCDTPQCNAL----EVSECRNA---------TC-LYEVSYGDGSYTVGDFATETLT 265
Query: 201 FPNKKITNFVVGCSFLS---IHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSPH 260
+ + N VGC + +G+ G G G +LPSQ+ F+YCL R DS
Sbjct: 266 IGSTLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSFSYCLVDR---DS-- 325
Query: 261 AGELILDSSGAKTSGLTYTPFR-QNPSVSNHAYKEYYYLTIRKIFVGNKAVKVPYKYLVP 320
DS+ G + +P P + NH +YYL + I VG + +++P
Sbjct: 326 ------DSASTVDFGTSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEM 385
Query: 321 GPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATDVESLTGLRPCFDISKDK 380
G+GG IIDSG+ T + ++ ++ K + +A V C+++S
Sbjct: 386 DESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFD---TCYNLSAKT 445
Query: 381 SVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAAESGGGPSIILGAFQQQ 440
+VE P + FH GG ALP NY V S G CL ++ + I+G QQQ
Sbjct: 446 TVEVPTVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLA------IIGNVQQQ 483
Query: 441 NFYVEYDLVNQKIGFRQQTC 456
V +DL N IGF C
Sbjct: 506 GTRVTFDLANSLIGFSSNKC 483
BLAST of Carg03639 vs. TAIR 10
Match:
AT2G42980.1 (Eukaryotic aspartyl protease family protein )
HSP 1 Score: 159.8 bits (403), Expect = 5.0e-39
Identity = 123/397 (30.98%), Postives = 178/397 (44.84%), Query Frame = 0
Query: 81 GAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCSECSFPKIDPARIPRFIPKLSSS 140
G Y + GTP + LI DTGS L WL C Y C + D PK S+S
Sbjct: 158 GEYFMDVLVGTPPKHFSLILDTGSDLNWLQCLPCYDCFHQNGMFYD--------PKTSAS 217
Query: 141 SKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVVQYGSGS-TAGLLLSETLD 200
K + C +P+C+ I PD QC S N Q+CP Y YG S T G ET
Sbjct: 218 FKNITCNDPRCSLISSPDPPVQCESDN-------QSCP-YFYWYGDRSNTTGDFAVETFT 277
Query: 201 F---------PNKKITNFVVGCSFLS---IHQPSGIAGFGRGSESLPSQMGL---KKFAY 260
K+ N + GC + SG+ G GRG S SQ+ F+Y
Sbjct: 278 VNLTTTEGGSSEYKVGNMMFGCGHWNRGLFSGASGLLGLGRGPLSFSSQLQSLYGHSFSY 337
Query: 261 CLASRKFDDSPHAGELIL--DSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRKIFVG 320
CL R ++ + +LI D + L +T F ++ + +YY+ I+ I VG
Sbjct: 338 CLVDRN-SNTNVSSKLIFGEDKDLLNHTNLNFTSFVNG---KENSVETFYYIQIKSILVG 397
Query: 321 NKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEI-EKQLANRTRATDVES 380
KA+ +P + DG+GG+IIDSG+T ++ +P +E + + EK N D
Sbjct: 398 GKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAEKMKENYPIFRDFPV 457
Query: 381 LTGLRPCFDIS--KDKSVEFPELTFHLKGGAKWALPLSNYFALVSSSGVACLTVVTHKAA 440
L PCF++S ++ ++ PEL G W P N F +S V + T K+
Sbjct: 458 LD---PCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLSEDLVCLAILGTPKST 517
Query: 441 ESGGGPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 457
S I+G +QQQNF++ YD ++GF C+
Sbjct: 518 FS------IIGNYQQQNFHILYDTKRSRLGFTPTKCA 525
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
KAG7017489.1 | 1.6e-265 | 100.00 | Aspartyl protease family protein 2, partial [Cucurbita argyrosperma subsp. argyr... | [more] |
KAG6580736.1 | 2.3e-264 | 99.56 | Aspartyl protease 25, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
XP_022934749.1 | 6.8e-264 | 99.56 | probable aspartyl protease At4g16563 [Cucurbita moschata] | [more] |
XP_023528159.1 | 1.7e-262 | 99.12 | probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo] | [more] |
XP_022982947.1 | 1.6e-257 | 96.71 | probable aspartyl protease At4g16563 [Cucurbita maxima] | [more] |
Match Name | E-value | Identity | Description | |
Q940R4 | 7.1e-46 | 29.98 | Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g1656... | [more] |
Q9LNJ3 | 3.5e-37 | 30.73 | Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... | [more] |
Q766C2 | 1.1e-33 | 32.47 | Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... | [more] |
Q766C3 | 1.4e-33 | 29.21 | Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... | [more] |
Q6F4N5 | 2.6e-32 | 27.25 | Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1F3G5 | 3.3e-264 | 99.56 | probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC1114418... | [more] |
A0A6J1IXY3 | 7.9e-258 | 96.71 | probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111481640... | [more] |
A0A5A7TRK2 | 1.1e-232 | 87.31 | Aspartic proteinase nepenthesin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... | [more] |
A0A1S3B6B5 | 1.1e-232 | 87.31 | aspartic proteinase nepenthesin-2 OS=Cucumis melo OX=3656 GN=LOC103486666 PE=3 S... | [more] |
A0A6J1FJU4 | 2.0e-232 | 87.80 | probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC1114460... | [more] |