Carg03574 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg03574
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	metal tolerance protein C2 isoform X3
Location	Carg_Chr14: 1909493 .. 1910775 (-)
RNA-Seq Expression	Carg03574
Synteny	Carg03574

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATACTGCATCAAATGATTCGCAGGATTCTTGGAATAGGGATACCGGATTTGGCGCCAATGATCGGAGGTTCGCTTTTTCGCGTCGAACTTCGTCTAAGCAATGGCAAGAGCCACCCCACACGCCGATTCCTATCAACTCCGACGATTCGGCGAAGCCTTTGCTTTCGCGGAATATGTCGAGCATAAACATTCCGCCTGGAAGCTACTTCGCTTCCGAAAGTAATAAATTCTATAGTGTAAAGGATAAATCAAGCGATAAATTATCGGTTTTGTCGCTCGCCTTTACAATTTTAGAGAATGTGAGATCTGGGAATCGGTATATGAAGAGGCTGTTTCTGATGATTTCGCTTAATGTCCTATATACAACTGCCGAGTTAGCTATTGGTCTCCTTACTGGCCGTATTGGTATGGACGTCACTATCCTTTTTCCTCTTTTAATTCACCATCCTTTATGTCGAAATTTATTCTTCGTGTGTGAGTAATTTATCTTTATTTTTCTTATGAATTCAGGTCTTGTTTCTGATGCATTTCATTTGACTTTTGGCTGTGGTCTTTTGACATTTTCATTGTTTGCCATTGCTGCATCTCGGAGTAAGCCTGATCATGTTTTCACCTACGGGTAAGGTTTTATTTGCTCATGCCTCTGAAGCCAACTTAAGTACACTAAGTTTTTAGTTAATTGTTACTATTTCGATCAGTTTTTACTCTGTTGAAAGTAGTTTCTTGGTATTTTTGAGTTCTGTGCCTAAATTTTAGGGATGCTTTTCCATTTCTCTCCCTAATCTTTTGTAAATTTCAATTTCATCATTCCCTTGTCTAACTTGTTATGAGTTGTGAAAGATGAACCCATGAAAAATGAAAGTATACTATTAACTGGCCATCTAATTAGAAAATACTAATTTTAAATTTTGCATCATCACTTTCATATATTGGTGTGATTGAAAAGTTACAAGTATATTGAGAACAAAATCATATATGAATTGGAACATAAATAAGGCGTCATAATTTGAGCACATATCAATGAACTTGCAAGTTTGTTTTGTTTTGGATATCTCACATACCTTTTCACGGTTTTCCGAACTTCATTTGATTACTAGAGACGGTTATAATTGTAGTGGTTTGTCTTACCAGGTACAAAAGACTAGAGGTTTTGGCTGCCTTTACTAATGCTGTAAGTATTCAAAATTTTACATGTTTATGCATATTTACTGTCCCTGGAACTCTTCTGTTGTATGCTATCCTTTTTTTATTGATGGCTGGTTGGTTCATGTTCTTTTGA

mRNA sequence

ATGGATACTGCATCAAATGATTCGCAGGATTCTTGGAATAGGGATACCGGATTTGGCGCCAATGATCGGAGGTTCGCTTTTTCGCGTCGAACTTCGTCTAAGCAATGGCAAGAGCCACCCCACACGCCGATTCCTATCAACTCCGACGATTCGGCGAAGCCTTTGCTTTCGCGGAATATGTCGAGCATAAACATTCCGCCTGGAAGCTACTTCGCTTCCGAAAGTAATAAATTCTATAGTGTAAAGGATAAATCAAGCGATAAATTATCGGTTTTGTCGCTCGCCTTTACAATTTTAGAGAATGTGAGATCTGGGAATCGGTATATGAAGAGGCTGTTTCTGATGATTTCGCTTAATGTCCTATATACAACTGCCGAGTTAGCTATTGGTCTCCTTACTGGCCGTATTGGTCTTGTTTCTGATGCATTTCATTTGACTTTTGGCTGTGGTCTTTTGACATTTTCATTGTTTGCCATTGCTGCATCTCGGAGTAAGCCTGATCATGTTTTCACCTACGGGTACAAAAGACTAGAGGTTTTGGCTGCCTTTACTAATGCTGTAAGTATTCAAAATTTTACATGTTTATGCATATTTACTGTCCCTGGAACTCTTCTGTTGTATGCTATCCTTTTTTTATTGATGGCTGGTTGGTTCATGTTCTTTTGA

Coding sequence (CDS)

ATGGATACTGCATCAAATGATTCGCAGGATTCTTGGAATAGGGATACCGGATTTGGCGCCAATGATCGGAGGTTCGCTTTTTCGCGTCGAACTTCGTCTAAGCAATGGCAAGAGCCACCCCACACGCCGATTCCTATCAACTCCGACGATTCGGCGAAGCCTTTGCTTTCGCGGAATATGTCGAGCATAAACATTCCGCCTGGAAGCTACTTCGCTTCCGAAAGTAATAAATTCTATAGTGTAAAGGATAAATCAAGCGATAAATTATCGGTTTTGTCGCTCGCCTTTACAATTTTAGAGAATGTGAGATCTGGGAATCGGTATATGAAGAGGCTGTTTCTGATGATTTCGCTTAATGTCCTATATACAACTGCCGAGTTAGCTATTGGTCTCCTTACTGGCCGTATTGGTCTTGTTTCTGATGCATTTCATTTGACTTTTGGCTGTGGTCTTTTGACATTTTCATTGTTTGCCATTGCTGCATCTCGGAGTAAGCCTGATCATGTTTTCACCTACGGGTACAAAAGACTAGAGGTTTTGGCTGCCTTTACTAATGCTGTAAGTATTCAAAATTTTACATGTTTATGCATATTTACTGTCCCTGGAACTCTTCTGTTGTATGCTATCCTTTTTTTATTGATGGCTGGTTGGTTCATGTTCTTTTGA

Protein sequence

MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNMSSINIPPGSYFASESNKFYSVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNVLYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVLAAFTNAVSIQNFTCLCIFTVPGTLLLYAILFLLMAGWFMFF

Homology

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BLAST of Carg03574 vs. NCBI nr
Match: KAG7017552.1 (Metal tolerance protein C2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 426.4 bits (1095), Expect = 1.5e-115
Identity = 221/221 (100.00%), Postives = 221/221 (100.00%), Query Frame = 0


Query: 1   MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM 60
           MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM
Sbjct: 1   MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM 60

Query: 61  SSINIPPGSYFASESNKFYSVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNV 120
           SSINIPPGSYFASESNKFYSVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNV
Sbjct: 61  SSINIPPGSYFASESNKFYSVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNV 120

Query: 121 LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL 180
           LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL
Sbjct: 121 LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL 180

Query: 181 AAFTNAVSIQNFTCLCIFTVPGTLLLYAILFLLMAGWFMFF 222
           AAFTNAVSIQNFTCLCIFTVPGTLLLYAILFLLMAGWFMFF
Sbjct: 181 AAFTNAVSIQNFTCLCIFTVPGTLLLYAILFLLMAGWFMFF 221

[Close]

BLAST of Carg03574 vs. NCBI nr
Match: KAG6580803.1 (Metal tolerance protein C2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 362.8 bits (930), Expect = 2.0e-96
Identity = 186/187 (99.47%), Postives = 187/187 (100.00%), Query Frame = 0


Query: 1   MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM 60
           MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM
Sbjct: 1   MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM 60

Query: 61  SSINIPPGSYFASESNKFYSVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNV 120
           SSINIPPGSYFASESNKFYSVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNV
Sbjct: 61  SSINIPPGSYFASESNKFYSVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNV 120

Query: 121 LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL 180
           LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL
Sbjct: 121 LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL 180

Query: 181 AAFTNAV 188
           AAFTNA+
Sbjct: 181 AAFTNAL 187

[Close]

BLAST of Carg03574 vs. NCBI nr
Match: XP_023526148.1 (metal tolerance protein C2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 360.9 bits (925), Expect = 7.7e-96
Identity = 184/187 (98.40%), Postives = 186/187 (99.47%), Query Frame = 0


Query: 1   MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM 60
           MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM
Sbjct: 1   MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM 60

Query: 61  SSINIPPGSYFASESNKFYSVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNV 120
           SSINIPPGSYFASESNKFYSVKDKSSDKLSVLSLAFTI ENVRSGNRYMKRLFLMISLNV
Sbjct: 61  SSINIPPGSYFASESNKFYSVKDKSSDKLSVLSLAFTIFENVRSGNRYMKRLFLMISLNV 120

Query: 121 LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL 180
           LYTTAELA+GLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL
Sbjct: 121 LYTTAELAVGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL 180

Query: 181 AAFTNAV 188
           AAFTNA+
Sbjct: 181 AAFTNAL 187

[Close]

BLAST of Carg03574 vs. NCBI nr
Match: XP_023526149.1 (metal tolerance protein C2 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 360.9 bits (925), Expect = 7.7e-96
Identity = 184/187 (98.40%), Postives = 186/187 (99.47%), Query Frame = 0


Query: 1   MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM 60
           MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM
Sbjct: 1   MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM 60

Query: 61  SSINIPPGSYFASESNKFYSVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNV 120
           SSINIPPGSYFASESNKFYSVKDKSSDKLSVLSLAFTI ENVRSGNRYMKRLFLMISLNV
Sbjct: 61  SSINIPPGSYFASESNKFYSVKDKSSDKLSVLSLAFTIFENVRSGNRYMKRLFLMISLNV 120

Query: 121 LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL 180
           LYTTAELA+GLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL
Sbjct: 121 LYTTAELAVGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL 180

Query: 181 AAFTNAV 188
           AAFTNA+
Sbjct: 181 AAFTNAL 187

[Close]

BLAST of Carg03574 vs. NCBI nr
Match: XP_022934284.1 (metal tolerance protein C2 isoform X1 [Cucurbita moschata])

HSP 1 Score: 359.4 bits (921), Expect = 2.3e-95
Identity = 185/187 (98.93%), Postives = 186/187 (99.47%), Query Frame = 0


Query: 1   MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM 60
           MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM
Sbjct: 1   MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM 60

Query: 61  SSINIPPGSYFASESNKFYSVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNV 120
           SSINIPPGSYFASESNKF SVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNV
Sbjct: 61  SSINIPPGSYFASESNKFCSVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNV 120

Query: 121 LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL 180
           LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL
Sbjct: 121 LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL 180

Query: 181 AAFTNAV 188
           AAFTNA+
Sbjct: 181 AAFTNAL 187

[Close]

BLAST of Carg03574 vs. ExPASy Swiss-Prot
Match: Q6ICY4 (Metal tolerance protein C2 OS=Arabidopsis thaliana OX=3702 GN=MTPC2 PE=2 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 1.5e-46
Identity = 104/183 (56.83%), Postives = 130/183 (71.04%), Query Frame = 0


Query: 6   NDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNMSSINI 65
           N+  D  + D G+ ANDRR A+SR   S Q    P TP      ++AKP L R +SSI++
Sbjct: 13  NELPDDRSSDVGYAANDRRLAYSR---SFQQSHGPRTPA---VTEAAKPFLDRTVSSIDM 72

Query: 66  PPGSYFASESNKFYSV-KDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNVLYTT 125
           PP  Y    S+ F+   KD    K+SVL + + +   V SGNR MKRLFL+I+LNVLY+T
Sbjct: 73  PPDIYSVDGSDVFFGEGKDVDMAKVSVLEMVWEVFGVVTSGNRQMKRLFLLIALNVLYST 132

Query: 126 AELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVLAAFT 185
            EL+IG+ TGR+GLVSDAFHLTFGCGLLTFSLFA+A SR KPDH ++YGYKRLEVL+AFT
Sbjct: 133 TELSIGIFTGRVGLVSDAFHLTFGCGLLTFSLFAMATSRKKPDHAYSYGYKRLEVLSAFT 189

Query: 186 NAV 188
           NA+
Sbjct: 193 NAL 189

[Close]

BLAST of Carg03574 vs. ExPASy Swiss-Prot
Match: Q8TAD4 (Zinc transporter 5 OS=Homo sapiens OX=9606 GN=SLC30A5 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-12
Identity = 34/78 (43.59%), Postives = 50/78 (64.10%), Query Frame = 0


Query: 110 KRLFLMISLNVLYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHV 169
           +++F  + LN+L+T  EL  G+LT  +GL+SD FH+ F C  L   LFA   SR K   +
Sbjct: 417 RQIFYFLCLNLLFTFVELFYGVLTNSLGLISDGFHMLFDCSALVMGLFAALMSRWKATRI 476

Query: 170 FTYGYKRLEVLAAFTNAV 188
           F+YGY R+E+L+ F N +
Sbjct: 477 FSYGYGRIEILSGFINGL 494

[Close]

BLAST of Carg03574 vs. ExPASy Swiss-Prot
Match: Q8R4H9 (Zinc transporter 5 OS=Mus musculus OX=10090 GN=Slc30a5 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-12
Identity = 34/78 (43.59%), Postives = 50/78 (64.10%), Query Frame = 0


Query: 110 KRLFLMISLNVLYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHV 169
           +++F  + LN+L+T  EL  G+LT  +GL+SD FH+ F C  L   LFA   SR K   +
Sbjct: 415 RQIFYFLCLNLLFTFVELFYGVLTNSLGLISDGFHMLFDCSALVMGLFAALMSRWKATRI 474

Query: 170 FTYGYKRLEVLAAFTNAV 188
           F+YGY R+E+L+ F N +
Sbjct: 475 FSYGYGRIEILSGFINGL 492

[Close]

BLAST of Carg03574 vs. ExPASy Swiss-Prot
Match: Q5ZLF4 (Zinc transporter 5 OS=Gallus gallus OX=9031 GN=SLC30A5 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 6.1e-11
Identity = 31/78 (39.74%), Postives = 48/78 (61.54%), Query Frame = 0


Query: 110 KRLFLMISLNVLYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHV 169
           +++F  + LN+ +T  EL  G+ T  +GL+SD FH+ F C  L   LFA   +R K   +
Sbjct: 414 RQIFYFLCLNLAFTFVELFYGVWTNSLGLISDGFHMLFDCSALVMGLFAALMTRWKATRI 473

Query: 170 FTYGYKRLEVLAAFTNAV 188
           F+YGY R+E+L+ F N +
Sbjct: 474 FSYGYGRVEILSGFINGL 491

[Close]

BLAST of Carg03574 vs. ExPASy Swiss-Prot
Match: Q6DG36 (Zinc transporter 5 OS=Danio rerio OX=7955 GN=slc30a5 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.4e-10
Identity = 30/78 (38.46%), Postives = 48/78 (61.54%), Query Frame = 0


Query: 110 KRLFLMISLNVLYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHV 169
           +++F  + LN+ +T  EL  G+ T  +GL+SD FH+ F C  L   LFA   +R K   +
Sbjct: 413 RQIFYFLCLNLAFTFVELFYGVWTNSLGLISDGFHMLFDCSALVLGLFAALMTRWKATRI 472

Query: 170 FTYGYKRLEVLAAFTNAV 188
           ++YGY R+E+L+ F N +
Sbjct: 473 YSYGYGRVEILSGFINGL 490

[Close]

BLAST of Carg03574 vs. ExPASy TrEMBL
Match: A0A6J1F790 (metal tolerance protein C2 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111441494 PE=4 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 1.1e-95
Identity = 185/187 (98.93%), Postives = 186/187 (99.47%), Query Frame = 0


Query: 1   MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM 60
           MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM
Sbjct: 1   MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM 60

Query: 61  SSINIPPGSYFASESNKFYSVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNV 120
           SSINIPPGSYFASESNKF SVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNV
Sbjct: 61  SSINIPPGSYFASESNKFCSVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNV 120

Query: 121 LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL 180
           LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL
Sbjct: 121 LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL 180

Query: 181 AAFTNAV 188
           AAFTNA+
Sbjct: 181 AAFTNAL 187

[Close]

BLAST of Carg03574 vs. ExPASy TrEMBL
Match: A0A6J1F256 (metal tolerance protein C2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441494 PE=4 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 1.1e-95
Identity = 185/187 (98.93%), Postives = 186/187 (99.47%), Query Frame = 0


Query: 1   MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM 60
           MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM
Sbjct: 1   MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM 60

Query: 61  SSINIPPGSYFASESNKFYSVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNV 120
           SSINIPPGSYFASESNKF SVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNV
Sbjct: 61  SSINIPPGSYFASESNKFCSVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNV 120

Query: 121 LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL 180
           LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL
Sbjct: 121 LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL 180

Query: 181 AAFTNAV 188
           AAFTNA+
Sbjct: 181 AAFTNAL 187

[Close]

BLAST of Carg03574 vs. ExPASy TrEMBL
Match: A0A6J1F1E4 (metal tolerance protein C2 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111441494 PE=4 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 1.1e-95
Identity = 185/187 (98.93%), Postives = 186/187 (99.47%), Query Frame = 0


Query: 1   MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM 60
           MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM
Sbjct: 1   MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM 60

Query: 61  SSINIPPGSYFASESNKFYSVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNV 120
           SSINIPPGSYFASESNKF SVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNV
Sbjct: 61  SSINIPPGSYFASESNKFCSVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNV 120

Query: 121 LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL 180
           LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL
Sbjct: 121 LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL 180

Query: 181 AAFTNAV 188
           AAFTNA+
Sbjct: 181 AAFTNAL 187

[Close]

BLAST of Carg03574 vs. ExPASy TrEMBL
Match: A0A6J1J0V2 (metal tolerance protein C2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481659 PE=4 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 9.2e-95
Identity = 181/187 (96.79%), Postives = 186/187 (99.47%), Query Frame = 0


Query: 1   MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM 60
           MDTASNDSQDSWNRDTGFGANDRRFAFSR+TSSKQWQ+PPHTPIPINSDDSAKPLLSRNM
Sbjct: 1   MDTASNDSQDSWNRDTGFGANDRRFAFSRQTSSKQWQDPPHTPIPINSDDSAKPLLSRNM 60

Query: 61  SSINIPPGSYFASESNKFYSVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNV 120
           SSINIPPGSYFASESNKF+ VKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLN+
Sbjct: 61  SSINIPPGSYFASESNKFFCVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNI 120

Query: 121 LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL 180
           LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL
Sbjct: 121 LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL 180

Query: 181 AAFTNAV 188
           AAFTNA+
Sbjct: 181 AAFTNAL 187

[Close]

BLAST of Carg03574 vs. ExPASy TrEMBL
Match: A0A6J1J6G9 (metal tolerance protein C2 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111481659 PE=4 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 9.2e-95
Identity = 181/187 (96.79%), Postives = 186/187 (99.47%), Query Frame = 0


Query: 1   MDTASNDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNM 60
           MDTASNDSQDSWNRDTGFGANDRRFAFSR+TSSKQWQ+PPHTPIPINSDDSAKPLLSRNM
Sbjct: 1   MDTASNDSQDSWNRDTGFGANDRRFAFSRQTSSKQWQDPPHTPIPINSDDSAKPLLSRNM 60

Query: 61  SSINIPPGSYFASESNKFYSVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNV 120
           SSINIPPGSYFASESNKF+ VKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLN+
Sbjct: 61  SSINIPPGSYFASESNKFFCVKDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNI 120

Query: 121 LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL 180
           LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL
Sbjct: 121 LYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVL 180

Query: 181 AAFTNAV 188
           AAFTNA+
Sbjct: 181 AAFTNAL 187

[Close]

BLAST of Carg03574 vs. TAIR 10
Match: AT3G12100.1 (Cation efflux family protein )

HSP 1 Score: 187.6 bits (475), Expect = 1.1e-47
Identity = 104/183 (56.83%), Postives = 130/183 (71.04%), Query Frame = 0


Query: 6   NDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNMSSINI 65
           N+  D  + D G+ ANDRR A+SR   S Q    P TP      ++AKP L R +SSI++
Sbjct: 13  NELPDDRSSDVGYAANDRRLAYSR---SFQQSHGPRTPA---VTEAAKPFLDRTVSSIDM 72

Query: 66  PPGSYFASESNKFYSV-KDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNVLYTT 125
           PP  Y    S+ F+   KD    K+SVL + + +   V SGNR MKRLFL+I+LNVLY+T
Sbjct: 73  PPDIYSVDGSDVFFGEGKDVDMAKVSVLEMVWEVFGVVTSGNRQMKRLFLLIALNVLYST 132

Query: 126 AELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVLAAFT 185
            EL+IG+ TGR+GLVSDAFHLTFGCGLLTFSLFA+A SR KPDH ++YGYKRLEVL+AFT
Sbjct: 133 TELSIGIFTGRVGLVSDAFHLTFGCGLLTFSLFAMATSRKKPDHAYSYGYKRLEVLSAFT 189

Query: 186 NAV 188
           NA+
Sbjct: 193 NAL 189

[Close]

BLAST of Carg03574 vs. TAIR 10
Match: AT3G12100.2 (Cation efflux family protein )

HSP 1 Score: 187.6 bits (475), Expect = 1.1e-47
Identity = 104/183 (56.83%), Postives = 130/183 (71.04%), Query Frame = 0


Query: 6   NDSQDSWNRDTGFGANDRRFAFSRRTSSKQWQEPPHTPIPINSDDSAKPLLSRNMSSINI 65
           N+  D  + D G+ ANDRR A+SR   S Q    P TP      ++AKP L R +SSI++
Sbjct: 13  NELPDDRSSDVGYAANDRRLAYSR---SFQQSHGPRTPA---VTEAAKPFLDRTVSSIDM 72

Query: 66  PPGSYFASESNKFYSV-KDKSSDKLSVLSLAFTILENVRSGNRYMKRLFLMISLNVLYTT 125
           PP  Y    S+ F+   KD    K+SVL + + +   V SGNR MKRLFL+I+LNVLY+T
Sbjct: 73  PPDIYSVDGSDVFFGEGKDVDMAKVSVLEMVWEVFGVVTSGNRQMKRLFLLIALNVLYST 132

Query: 126 AELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHVFTYGYKRLEVLAAFT 185
            EL+IG+ TGR+GLVSDAFHLTFGCGLLTFSLFA+A SR KPDH ++YGYKRLEVL+AFT
Sbjct: 133 TELSIGIFTGRVGLVSDAFHLTFGCGLLTFSLFAMATSRKKPDHAYSYGYKRLEVLSAFT 189

Query: 186 NAV 188
           NA+
Sbjct: 193 NAL 189

[Close]

BLAST of Carg03574 vs. TAIR 10
Match: AT2G04620.1 (Cation efflux family protein )

HSP 1 Score: 60.5 bits (145), Expect = 2.0e-09
Identity = 28/78 (35.90%), Postives = 43/78 (55.13%), Query Frame = 0


Query: 110 KRLFLMISLNVLYTTAELAIGLLTGRIGLVSDAFHLTFGCGLLTFSLFAIAASRSKPDHV 169
           +++ L + +N  Y   E   G ++  +GL+SDA H+ F C  L   L+A   SR   +H 
Sbjct: 418 RKIALFLLINTAYMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANHQ 477

Query: 170 FTYGYKRLEVLAAFTNAV 188
           + YG  R EVL+ + NAV
Sbjct: 478 YNYGRGRFEVLSGYVNAV 495

The following BLAST results are available for this feature:

BLAST of Carg03574 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7017552.1	1.5e-115	100.00	Metal tolerance protein C2, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6580803.1	2.0e-96	99.47	Metal tolerance protein C2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023526148.1	7.7e-96	98.40	metal tolerance protein C2 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_023526149.1	7.7e-96	98.40	metal tolerance protein C2 isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_022934284.1	2.3e-95	98.93	metal tolerance protein C2 isoform X1 [Cucurbita moschata]	[more]

BLAST of Carg03574 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6ICY4	1.5e-46	56.83	Metal tolerance protein C2 OS=Arabidopsis thaliana OX=3702 GN=MTPC2 PE=2 SV=1	[more]
Q8TAD4	1.1e-12	43.59	Zinc transporter 5 OS=Homo sapiens OX=9606 GN=SLC30A5 PE=1 SV=1	[more]
Q8R4H9	1.1e-12	43.59	Zinc transporter 5 OS=Mus musculus OX=10090 GN=Slc30a5 PE=1 SV=1	[more]
Q5ZLF4	6.1e-11	39.74	Zinc transporter 5 OS=Gallus gallus OX=9031 GN=SLC30A5 PE=2 SV=1	[more]
Q6DG36	1.4e-10	38.46	Zinc transporter 5 OS=Danio rerio OX=7955 GN=slc30a5 PE=2 SV=1	[more]

BLAST of Carg03574 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F790	1.1e-95	98.93	metal tolerance protein C2 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111441...	[more]
A0A6J1F256	1.1e-95	98.93	metal tolerance protein C2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441...	[more]
A0A6J1F1E4	1.1e-95	98.93	metal tolerance protein C2 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111441...	[more]
A0A6J1J0V2	9.2e-95	96.79	metal tolerance protein C2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11148165...	[more]
A0A6J1J6G9	9.2e-95	96.79	metal tolerance protein C2 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC11148165...	[more]

BLAST of Carg03574 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12100.1	1.1e-47	56.83	Cation efflux family protein	[more]
AT3G12100.2	1.1e-47	56.83	Cation efflux family protein	[more]
AT2G04620.1	2.0e-09	35.90	Cation efflux family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027469	Cation efflux transmembrane domain superfamily	GENE3D	1.20.1510.10	Cation efflux protein transmembrane domain	coord: 108..220 e-value: 1.8E-19 score: 72.1
IPR027469	Cation efflux transmembrane domain superfamily	SUPERFAMILY	161111	Cation efflux protein transmembrane domain-like	coord: 106..214
IPR002524	Cation efflux protein	PFAM	PF01545	Cation_efflux	coord: 112..195 e-value: 3.2E-15 score: 56.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 30..44
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..50
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..16
None	No IPR available	PANTHER	PTHR45755:SF3	METAL TOLERANCE PROTEIN C2	coord: 5..187
None	No IPR available	PANTHER	PTHR45755	FAMILY NOT NAMED	coord: 5..187

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg03574-RA	Carg03574-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098655	cation transmembrane transport
biological_process	GO:0006812	cation transport
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0005794	Golgi apparatus
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008324	cation transmembrane transporter activity