Carg03533 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg03533 vs. NCBI nr
Match: KAG7017593.1 (putative lysophospholipase BODYGUARD 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 919.5 bits (2375), Expect = 1.1e-263
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   MCFFTAVGFSMSELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFIDAILCVIYR 60
           MCFFTAVGFSMSELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFIDAILCVIYR
Sbjct: 1   MCFFTAVGFSMSELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFIDAILCVIYR 60

Query: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSE 120
           YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSE
Sbjct: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSE 120

Query: 121 KMNSGSAWNRWSDCGCSSCVDGKENRNQKLYVAVREPPRGRSEKPKENVIFLHGFVSSSS 180
           KMNSGSAWNRWSDCGCSSCVDGKENRNQKLYVAVREPPRGRSEKPKENVIFLHGFVSSSS
Sbjct: 121 KMNSGSAWNRWSDCGCSSCVDGKENRNQKLYVAVREPPRGRSEKPKENVIFLHGFVSSSS 180

Query: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240
           LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS
Sbjct: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240

Query: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLF 300
           FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLF
Sbjct: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLF 300

Query: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNINFRVIDLTKHTHHSAWHSMHN 360
           GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNINFRVIDLTKHTHHSAWHSMHN
Sbjct: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNINFRVIDLTKHTHHSAWHSMHN 360

Query: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQ 420
           VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQ
Sbjct: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQ 420

Query: 421 TIILGRERELTQELEYIWANTVDIERS 448
           TIILGRERELTQELEYIWANTVDIERS
Sbjct: 421 TIILGRERELTQELEYIWANTVDIERS 447

BLAST of Carg03533 vs. NCBI nr
Match: XP_022934164.1 (probable lysophospholipase BODYGUARD 4 isoform X1 [Cucurbita moschata])

HSP 1 Score: 899.4 bits (2323), Expect = 1.2e-257
Identity = 436/442 (98.64%), Postives = 439/442 (99.32%), Query Frame = 0

Query: 1   MCFFTAVGFSMSELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFIDAILCVIYR 60
           MCFFTAVGFS+SELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDF+DAILCVIYR
Sbjct: 1   MCFFTAVGFSVSELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYR 60

Query: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSE 120
           YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSE
Sbjct: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSE 120

Query: 121 KMNSGSAWNRWSDCGCSSCVDGKENRNQKLYVAVREPPRGRSEKPKENVIFLHGFVSSSS 180
           KMNSGSAWNRWSDCGCSSCVDGKENRNQKLYVA REPPRGRS KPKENVIFLHGFVSSSS
Sbjct: 121 KMNSGSAWNRWSDCGCSSCVDGKENRNQKLYVAAREPPRGRSGKPKENVIFLHGFVSSSS 180

Query: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240
           LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS
Sbjct: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240

Query: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLF 300
           FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAK VWPPLLF
Sbjct: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKSVWPPLLF 300

Query: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNINFRVIDLTKHTHHSAWHSMHN 360
           GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNI+FRVIDLTKHTHHSAWHSMHN
Sbjct: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHN 360

Query: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQ 420
           VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQ
Sbjct: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQ 420

Query: 421 TIILGRERELTQELEYIWANTV 443
           TIILGRERELTQELEYIWANTV
Sbjct: 421 TIILGRERELTQELEYIWANTV 442

BLAST of Carg03533 vs. NCBI nr
Match: XP_023526531.1 (probable lysophospholipase BODYGUARD 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 885.9 bits (2288), Expect = 1.4e-253
Identity = 428/447 (95.75%), Postives = 437/447 (97.76%), Query Frame = 0

Query: 1   MCFFTAVGFSMSELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFIDAILCVIYR 60
           MCFFTAVGFS   + FL MDSSISSRKWLIRFRTSLLDFVNFTVFLFLDF+DAILCVIYR
Sbjct: 1   MCFFTAVGFSDERVDFLFMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYR 60

Query: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSE 120
           YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETL GRR+VFRRIALVGFSGSCKDSE
Sbjct: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLCGRRNVFRRIALVGFSGSCKDSE 120

Query: 121 KMNSGSAWNRWSDCGCSSCVDGKENRNQKLYVAVREPPRGRSEKPKENVIFLHGFVSSSS 180
           KMN+GSAWNRWSDCGCSSCVDG ENRNQKLYVAVREPPRGRS KPKENVIFLHGFVSSSS
Sbjct: 121 KMNNGSAWNRWSDCGCSSCVDGMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSS 180

Query: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240
           LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS
Sbjct: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240

Query: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLF 300
           FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLF
Sbjct: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLF 300

Query: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNINFRVIDLTKHTHHSAWHSMHN 360
           GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNI+FRVIDLTKHTHHSAWHSMHN
Sbjct: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHN 360

Query: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQ 420
           VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAP+ECSYNLKKKAVDATVN V+N DHQ
Sbjct: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVQNVDHQ 420

Query: 421 TIILGRERELTQELEYIWANTVDIERS 448
           TIILGRERELTQ+LEYIWANTV+IERS
Sbjct: 421 TIILGRERELTQDLEYIWANTVEIERS 447

BLAST of Carg03533 vs. NCBI nr
Match: XP_022934165.1 (probable lysophospholipase BODYGUARD 4 isoform X2 [Cucurbita moschata])

HSP 1 Score: 885.2 bits (2286), Expect = 2.4e-253
Identity = 432/442 (97.74%), Postives = 434/442 (98.19%), Query Frame = 0

Query: 1   MCFFTAVGFSMSELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFIDAILCVIYR 60
           MCFFTAVGFS+SELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDF+DAILCVIYR
Sbjct: 1   MCFFTAVGFSVSELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYR 60

Query: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSE 120
           YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSE
Sbjct: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSE 120

Query: 121 KMNSGSAWNRWSDCGCSSCVDGKENRNQKLYVAVREPPRGRSEKPKENVIFLHGFVSSSS 180
           KMNSGSAWNRWSDCGCSSCVDGKENRNQKLYVA REPPRGRS KPKENVIFLHGFVSSSS
Sbjct: 121 KMNSGSAWNRWSDCGCSSCVDGKENRNQKLYVAAREPPRGRSGKPKENVIFLHGFVSSSS 180

Query: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240
           LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS
Sbjct: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240

Query: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLF 300
           FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAK VWPPLLF
Sbjct: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKSVWPPLLF 300

Query: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNINFRVIDLTKHTHHSAWHSMHN 360
           GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPR     RVIDLTKHTHHSAWHSMHN
Sbjct: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPR-----RVIDLTKHTHHSAWHSMHN 360

Query: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQ 420
           VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQ
Sbjct: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQ 420

Query: 421 TIILGRERELTQELEYIWANTV 443
           TIILGRERELTQELEYIWANTV
Sbjct: 421 TIILGRERELTQELEYIWANTV 437

BLAST of Carg03533 vs. NCBI nr
Match: KAG6580837.1 (putative lysophospholipase BODYGUARD 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 877.9 bits (2267), Expect = 3.8e-251
Identity = 424/429 (98.83%), Postives = 426/429 (99.30%), Query Frame = 0

Query: 19  MDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFIDAILCVIYRYIDQFLEGKTTACYCGSR 78
           MDSSISSRKWLIRFRTSLLDFVNFTVFLFLDF+DAILCVIYRYIDQFLEGKTTACYCGSR
Sbjct: 1   MDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYRYIDQFLEGKTTACYCGSR 60

Query: 79  GEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSEKMNSGSAWNRWSDCGCSS 138
           GEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSEKMNSGSAWNRWSDCGCSS
Sbjct: 61  GEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSEKMNSGSAWNRWSDCGCSS 120

Query: 139 CVDGKENRNQKLYVAVREPPRGRSEKPKENVIFLHGFVSSSSLWTESVFPNLSETQKLNY 198
           CVDGKENRNQKLYVA REPPRGRS KPKENVIFLHGFVSSSSLWTESVFPNLSETQKLNY
Sbjct: 121 CVDGKENRNQKLYVAAREPPRGRSGKPKENVIFLHGFVSSSSLWTESVFPNLSETQKLNY 180

Query: 199 RLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMGCLIALALAA 258
           RLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMGCLIALA+AA
Sbjct: 181 RLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMGCLIALAIAA 240

Query: 259 KYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACFV 318
           KYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACFV
Sbjct: 241 KYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACFV 300

Query: 319 ICRNHRIWEWILRRINPRRNINFRVIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDELSR 378
           ICRNHRIWEWILRRINPRRNINFRVIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDELSR
Sbjct: 301 ICRNHRIWEWILRRINPRRNINFRVIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDELSR 360

Query: 379 AGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQTIILGRERELTQELEYIW 438
           AGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQTIILGRERELTQEL YIW
Sbjct: 361 AGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQTIILGRERELTQELGYIW 420

Query: 439 ANTVDIERS 448
           ANTVDIERS
Sbjct: 421 ANTVDIERS 429

BLAST of Carg03533 vs. ExPASy Swiss-Prot
Match: Q700D5 (Probable lysophospholipase BODYGUARD 4 OS=Arabidopsis thaliana OX=3702 GN=BDG4 PE=2 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 7.0e-123
Identity = 213/419 (50.84%), Postives = 294/419 (70.17%), Query Frame = 0

Query: 31  RFRTSLLDFVNFTVFLFLDFIDAILCVIYRYIDQFLEGKTTACYCGSRGEERGNADGDGE 90
           +F T++   ++F VF FLD +DAILCV+Y ++D+ LE  +T CYC +   +    D   E
Sbjct: 6   KFGTAIHAALSFIVFFFLDLLDAILCVVYEFVDEILEENSTGCYCTAAAPQSQTTD---E 65

Query: 91  NEL-SETLYGRRSVFRRIALVGFSGSCKD--SEKMNSG----SAWNRWSDCGCSSCVDGK 150
           NEL SETL+GRR++FR +  +GF+   K   S K+        + NRWSDCGC SC    
Sbjct: 66  NELSSETLFGRRNIFRGMWFLGFAREFKSKLSRKLRKSKIHQESVNRWSDCGCKSCKSWT 125

Query: 151 ENRNQKLYVAVREPPRGR--SEKPKENVIFLHGFVSSSSLWTESVFPNLSETQKLNYRLF 210
           +N +  L+V V++        ++P ENVIF+HGF+ SS  WTE+VF ++   QK +YRL 
Sbjct: 126 KNEDGNLHVVVKDSTSREYSVQEPSENVIFIHGFMGSSHFWTETVFEHI---QKDDYRLL 185

Query: 211 AVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMGCLIALALAAKYS 270
           A+DLLGFG SPKPRD  YT+KDH++ IE SVI  + L+SFH+VAHSMGCLIALALAAK+S
Sbjct: 186 AIDLLGFGESPKPRDSLYTLKDHVDTIERSVIKPYQLDSFHVVAHSMGCLIALALAAKHS 245

Query: 271 NSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACFVICR 330
           N VKS+TLVAPPYFPS   G  +VL  +  KR+WPPL FG++VMSWYEH+GRC CF+IC+
Sbjct: 246 NIVKSVTLVAPPYFPSSVEG--SVLNRIARKRLWPPLAFGTAVMSWYEHIGRCVCFIICK 305

Query: 331 NHRIWEWILRRINPRRNINFRVIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDELSRAGV 390
           +H+IWEW+++    +R I++++ D+T+HTHHSAWHSMHNVICGG+K+ D +L+ L ++GV
Sbjct: 306 HHKIWEWLIKLCIGKREIHWKIKDITRHTHHSAWHSMHNVICGGSKVADEHLETLIKSGV 365

Query: 391 KIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQTIILGRERELTQELEYIWAN 441
           KI++  GD D + P  CS N+K+      V+ +  ADH ++I GR  E  ++LE IW +
Sbjct: 366 KIHLMQGDCDQIVPSHCSGNMKRTFPAVEVDIITGADHDSMISGRGEEFAEKLESIWCS 416

BLAST of Carg03533 vs. ExPASy Swiss-Prot
Match: Q9FN79 (Probable lysophospholipase BODYGUARD 5 OS=Arabidopsis thaliana OX=3702 GN=BDG5 PE=3 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 5.6e-104
Identity = 195/424 (45.99%), Postives = 266/424 (62.74%), Query Frame = 0

Query: 41  NFTVFLFLDFIDAILCVIYRYIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGR 100
           ++ VF   D +D  LC+++R++D+ +E K+ +C+C +  E+   A+ +    LSETLY R
Sbjct: 19  SWAVFFLFDLLDYFLCIVFRFLDEVMEEKSESCHCNNPREKTHFAEYE---FLSETLYRR 78

Query: 101 RSVFRRIALVGFSGSCKDSEK----------------MN--SGSAWNRWSDCGCSSCVDG 160
           R+VFR+   + F+    +  K                MN  S    NRWSDCGC +CV  
Sbjct: 79  RNVFRQAGFLRFANKLPEITKKIGIVTFLRKFLFPHTMNKVSHEVANRWSDCGCKTCVSW 138

Query: 161 KENRNQKLYVAVREP---PRGRSEKPKENVIFLHGFVSSSSLWTESVFPNLSE-TQKLNY 220
                 KL V V++P       S KP ENVIF+HGF++SSS WT +VF  L E T+  NY
Sbjct: 139 --INTDKLNVIVKQPSISDLSISNKPVENVIFVHGFLASSSFWTNTVFKYLPETTEGTNY 198

Query: 221 RLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMGCLIALALAA 280
           R FA+DLLGFG SPKPR   Y++K+H+E IE+SVI    L SFH+VAHSMGC+I +ALAA
Sbjct: 199 RFFAIDLLGFGDSPKPRASQYSLKEHVEMIEKSVILPNNLTSFHVVAHSMGCIIGIALAA 258

Query: 281 KYSNSVKSITLVAPPYF-PSKDGGAMTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACF 340
           K+S+SVKS+ LVAPPYF  SK G +   L+ +  K++WPP  F +++M WYEH+GR  C 
Sbjct: 259 KFSDSVKSVALVAPPYFADSKGGASCAALDVVAKKKLWPPASFFTAMMCWYEHIGRGVCL 318

Query: 341 VICRNHRIWEWILRRINPRRNINFRVIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDELS 400
           V CR+HR WE I++ +  RR +   ++D TKHTH S WHSMHNVICGGAK TD +L+ L 
Sbjct: 319 VFCRHHRTWERIIKIVTWRRKLPTAIMDFTKHTHQSGWHSMHNVICGGAKFTDKHLETLI 378

Query: 401 RAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQTIILGRERELTQELEYI 442
           ++GVKI +  GD+DVV PI+C  N+K K     V  +   DH T+I+ R       L  +
Sbjct: 379 KSGVKINVMQGDKDVVVPIDCLSNMKGKFPAVEVEVIAGTDHSTVIMSRREVFAANLVSL 437

BLAST of Carg03533 vs. ExPASy Swiss-Prot
Match: Q8LFX7 (Probable lysophospholipase BODYGUARD 1 OS=Arabidopsis thaliana OX=3702 GN=BDG1 PE=2 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 3.6e-95
Identity = 178/458 (38.86%), Postives = 264/458 (57.64%), Query Frame = 0

Query: 32  FRTSLLDFVNFTVFLFLDFIDAILCVIYRYIDQFLEGKTTACYC------------GSRG 91
           F  SL   V   VF  LD +D +LC  Y+ +D F E +   CYC            G+RG
Sbjct: 3   FSRSLNRTVGVFVFFILDIVDFLLCFTYKTLDFFFESEWKPCYCCPPPEAKPISAGGNRG 62

Query: 92  EERGNADGDGE--------------NELSETLYGRRSVFRRIALV------GFSGSCKDS 151
            +   ++  G+              +E+S+TLY R S+  ++  +           C +S
Sbjct: 63  GKMIVSERSGDYSKVVSLTRTKIYLDEISDTLYSRPSLLTKLTKLVKCFKKDVVKCCDES 122

Query: 152 EKMNSGSAWN----------------RWSDCGCSSCVDGKENRNQKLYVAVREPPRGRSE 211
           +K +  +                   RWSDC C+ C     + NQ L+V V++P      
Sbjct: 123 KKRSPSTKKTLLTVNSTVVEKLQRTPRWSDCHCTFCTSWLSSSNQSLFVNVQQP---TDN 182

Query: 212 KPKENVIFLHGFVSSSSLWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKD 271
           K +ENV+F+HGF+SSS+ WTE++FPN S++ K NYR  AVDLLG+G+SPKP D  YT+K+
Sbjct: 183 KAQENVVFIHGFLSSSTFWTETLFPNFSDSAKSNYRFLAVDLLGYGKSPKPNDSLYTLKE 242

Query: 272 HLEKIEESVIHQFGLNSFHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDG--G 331
           HLE IE SVI QF L +FHLVAHS+GC++ALALA K+  ++KS+TL+APPY+    G  G
Sbjct: 243 HLEMIERSVISQFRLKTFHLVAHSLGCILALALAVKHPGAIKSLTLLAPPYYSVPKGVQG 302

Query: 332 AMTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNINF 391
              V+  L  K VWPP+ FG+SV SWYEH+ R    V+C+NH + E++ R +   R   +
Sbjct: 303 TQYVMRRLAPKEVWPPMAFGASVASWYEHISRTVSLVLCKNHHLLEFLTRLLTRNRMRTY 362

Query: 392 RVIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDEL-SRAGVKIYIYHGDQDVVAPIECSY 439
            +     HTH+++WH++HN+I G     + YLD +      ++ ++HG +D + P+ECSY
Sbjct: 363 LIEGFLCHTHNASWHTLHNIIFGSGSKVEAYLDHVRDNVDCEVAVFHGGRDELIPVECSY 422

BLAST of Carg03533 vs. ExPASy Swiss-Prot
Match: Q9FJ24 (Probable lysophospholipase BODYGUARD 2 OS=Arabidopsis thaliana OX=3702 GN=BDG2 PE=2 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 5.4e-91
Identity = 178/460 (38.70%), Postives = 266/460 (57.83%), Query Frame = 0

Query: 40  VNFTVFLFLDFIDAILCVIYRYIDQFLEGKTTACYCGSRGEERGNAD--------GDGE- 99
           V F VF  LD  D +LC  Y+ +D FLE +   CYC S  E +   +        G  + 
Sbjct: 11  VGFFVFALLDIADFLLCYTYKTLDYFLESERKPCYCSSPPEAKAKTEKIIVSERGGYSKV 70

Query: 100 ----------NELSETLYGR--------RSVFRRIALVGFSG---------SC---KDSE 159
                     +E+S+TLY R          + R +    + G         SC   +  +
Sbjct: 71  VSLTRSKIHFDEISDTLYSRGPSLLTRLSKLVRSVKCFNYKGLIMRGNVVESCDHHESKK 130

Query: 160 KMNSG---------------SAWNRWSDCGCSSCVDGKENRNQ-KLYVAVREPPRGRSEK 219
           K++ G               S   RWSDC CS C     + N+  L+V V++P    ++K
Sbjct: 131 KISKGKKRLMTLNSTVIEKSSTAPRWSDCHCSFCTSWLTSTNRDSLFVKVQQPK--DNKK 190

Query: 220 PKENVIFLHGFVSSSSLWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDH 279
            ++NV+F+HGFVSSS+ WTE++FPN S++ K NYR  AVDLLG+GRSPKP D  YT+++H
Sbjct: 191 ARDNVVFIHGFVSSSAFWTETLFPNFSDSAKSNYRFIAVDLLGYGRSPKPNDSLYTLREH 250

Query: 280 LEKIEESVIHQFGLNSFHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDG--GA 339
           LE IE+SVI +F L +FH+VAHS+GC++ALALA K+  ++KS+TL+APPY+    G   A
Sbjct: 251 LEMIEKSVISKFKLKTFHIVAHSLGCILALALAVKHPGAIKSLTLLAPPYYKVPKGVQPA 310

Query: 340 MTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNINFR 399
             V+  +  K VWPP+ FG+SV+SWYEH+GR    V+ +NH++ E++ R +   R   + 
Sbjct: 311 QYVMREVARKEVWPPMQFGASVLSWYEHLGRTIGLVLIKNHQLIEFVTRLLTLNRMRTYL 370

Query: 400 VIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDEL-SRAGVKIYIYHGDQDVVAPIECSYN 442
           +     HTH+ ++H++HN+I G     D YLD +       + I+HG +D + P+ECSY+
Sbjct: 371 IEGFLCHTHNGSFHTLHNIIFGSGAKLDSYLDHVRDHVDCDVAIFHGGKDELIPVECSYS 430

BLAST of Carg03533 vs. ExPASy Swiss-Prot
Match: O22977 (Probable lysophospholipase BODYGUARD 3 OS=Arabidopsis thaliana OX=3702 GN=BDG3 PE=2 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 6.2e-87
Identity = 175/485 (36.08%), Postives = 269/485 (55.46%), Query Frame = 0

Query: 34  TSLLDFVNFTVFLFLDFIDAILCVIYRYIDQFLEGKTTACYCGSRGE----ERGN---AD 93
           T L + V+F VF  LD +D+ LC++Y+  D   E +   CYC S  E     RG    + 
Sbjct: 15  TWLNEAVSFVVFCILDIVDSFLCLLYKAADYLFEAEWKPCYCLSDKEPITTTRGKILLSH 74

Query: 94  GDGEN--------------------ELSETLYGRRSVFRRIALV---------------- 153
            +GE+                    ++SETLY R S+   I+ +                
Sbjct: 75  NNGESKILTLSPLQELGGRSKIELEDISETLYTRPSLISDISTISVNELNKRFVKVTRSE 134

Query: 154 ----GFSGSCKDSEK------------------------MNSGSAWNRWSDCGCSSCVD- 213
               G +   K+  +                         N     +RWSDC C  C   
Sbjct: 135 SECSGHNEKTKNKRRRSLTKSSLTVNFTVVEMLRGKIRPQNLNHDISRWSDCDCGFCTSW 194

Query: 214 -GKENRNQKLYVAVREPPRGRSEKPKENVIFLHGFVSSSSLWTESVFPNLSETQKLNYRL 273
               ++N  L+V  + P        KE+V+F+HGF+SSS+ WTE+VFP+LS +    +RL
Sbjct: 195 ASTSDKNHSLFVKTQIP---NGVTAKEDVLFIHGFISSSAFWTETVFPSLSASSS-THRL 254

Query: 274 FAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMGCLIALALAAKY 333
           FAVDLLGFG+SPKP D  YT+++H+E IE+SV+H++ + SFH+VAHS+GC++AL+LAA++
Sbjct: 255 FAVDLLGFGKSPKPADSLYTLREHVEMIEKSVLHKYNVKSFHIVAHSLGCILALSLAARH 314

Query: 334 SNSVKSITLVAPPYFPSKDG---GAMTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACF 393
              +KS+TL+APPY+P   G       V++ +  ++VWPP+  G+S+  WYEH+ R  C 
Sbjct: 315 GGLIKSLTLLAPPYYPVPKGEKKPRQYVMKKVAPRKVWPPIALGASMACWYEHISRTICL 374

Query: 394 VICRNHRIWEWI---LRRINPRRNINFRVIDLTKHTHHSAWHSMHNVICGGAKLTDGYLD 439
           +IC++HR+W++I   L R N  R +NF +     HTH++AWH++HN+ICG     D YLD
Sbjct: 375 LICKHHRVWQFIAGVLTRNN--RTVNFLIEGFMCHTHNAAWHTLHNIICGTGSKLDTYLD 434

BLAST of Carg03533 vs. ExPASy TrEMBL
Match: A0A6J1F1T1 (probable lysophospholipase BODYGUARD 4 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441414 PE=4 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 5.9e-258
Identity = 436/442 (98.64%), Postives = 439/442 (99.32%), Query Frame = 0

Query: 1   MCFFTAVGFSMSELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFIDAILCVIYR 60
           MCFFTAVGFS+SELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDF+DAILCVIYR
Sbjct: 1   MCFFTAVGFSVSELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYR 60

Query: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSE 120
           YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSE
Sbjct: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSE 120

Query: 121 KMNSGSAWNRWSDCGCSSCVDGKENRNQKLYVAVREPPRGRSEKPKENVIFLHGFVSSSS 180
           KMNSGSAWNRWSDCGCSSCVDGKENRNQKLYVA REPPRGRS KPKENVIFLHGFVSSSS
Sbjct: 121 KMNSGSAWNRWSDCGCSSCVDGKENRNQKLYVAAREPPRGRSGKPKENVIFLHGFVSSSS 180

Query: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240
           LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS
Sbjct: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240

Query: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLF 300
           FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAK VWPPLLF
Sbjct: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKSVWPPLLF 300

Query: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNINFRVIDLTKHTHHSAWHSMHN 360
           GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNI+FRVIDLTKHTHHSAWHSMHN
Sbjct: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHN 360

Query: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQ 420
           VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQ
Sbjct: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQ 420

Query: 421 TIILGRERELTQELEYIWANTV 443
           TIILGRERELTQELEYIWANTV
Sbjct: 421 TIILGRERELTQELEYIWANTV 442

BLAST of Carg03533 vs. ExPASy TrEMBL
Match: A0A6J1F6W3 (probable lysophospholipase BODYGUARD 4 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111441414 PE=4 SV=1)

HSP 1 Score: 885.2 bits (2286), Expect = 1.2e-253
Identity = 432/442 (97.74%), Postives = 434/442 (98.19%), Query Frame = 0

Query: 1   MCFFTAVGFSMSELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFIDAILCVIYR 60
           MCFFTAVGFS+SELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDF+DAILCVIYR
Sbjct: 1   MCFFTAVGFSVSELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYR 60

Query: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSE 120
           YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSE
Sbjct: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSE 120

Query: 121 KMNSGSAWNRWSDCGCSSCVDGKENRNQKLYVAVREPPRGRSEKPKENVIFLHGFVSSSS 180
           KMNSGSAWNRWSDCGCSSCVDGKENRNQKLYVA REPPRGRS KPKENVIFLHGFVSSSS
Sbjct: 121 KMNSGSAWNRWSDCGCSSCVDGKENRNQKLYVAAREPPRGRSGKPKENVIFLHGFVSSSS 180

Query: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240
           LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS
Sbjct: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240

Query: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLF 300
           FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAK VWPPLLF
Sbjct: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKSVWPPLLF 300

Query: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNINFRVIDLTKHTHHSAWHSMHN 360
           GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPR     RVIDLTKHTHHSAWHSMHN
Sbjct: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPR-----RVIDLTKHTHHSAWHSMHN 360

Query: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQ 420
           VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQ
Sbjct: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQ 420

Query: 421 TIILGRERELTQELEYIWANTV 443
           TIILGRERELTQELEYIWANTV
Sbjct: 421 TIILGRERELTQELEYIWANTV 437

BLAST of Carg03533 vs. ExPASy TrEMBL
Match: A0A6J1J489 (probable lysophospholipase BODYGUARD 4 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111481627 PE=4 SV=1)

HSP 1 Score: 852.8 bits (2202), Expect = 6.4e-244
Identity = 414/438 (94.52%), Postives = 423/438 (96.58%), Query Frame = 0

Query: 10  SMSELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFIDAILCVIYRYIDQFLEGK 69
           +MSELTFL MDSSI S KWLIRFRTSLLDFVNFTVFLFLDF+DAILCVIYRYIDQFLEGK
Sbjct: 42  AMSELTFLFMDSSILSGKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYRYIDQFLEGK 101

Query: 70  TTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSEKMNSGSAWN 129
           TTACYCGSRGEERGNADGDGENELSETLYGRR+VFRRIALVGFSGSCKDSEKMNSGSAWN
Sbjct: 102 TTACYCGSRGEERGNADGDGENELSETLYGRRNVFRRIALVGFSGSCKDSEKMNSGSAWN 161

Query: 130 RWSDCGCSSCVDGKENRNQKLYVAVREPPRGRSEKPKENVIFLHGFVSSSSLWTESVFPN 189
           RWSDCGCSSCVDG ENRNQKLYVAVREPPRGRS KPKENVIFLHGFVSSSSLWTESVFPN
Sbjct: 162 RWSDCGCSSCVDGMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSSLWTESVFPN 221

Query: 190 LSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMG 249
           LSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKI ESVIHQFGLNSFHLVAHSMG
Sbjct: 222 LSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKI-ESVIHQFGLNSFHLVAHSMG 281

Query: 250 CLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLFGSSVMSWYE 309
           CLIALALAAKYSNSVK+ITLVAPPYFPSK G AM VLENL AKR+WPPLLFGSSVMSWYE
Sbjct: 282 CLIALALAAKYSNSVKTITLVAPPYFPSKHGVAMAVLENLAAKRIWPPLLFGSSVMSWYE 341

Query: 310 HVGRCACFVICRNHRIWEWILRRINPRRNINFRVIDLTKHTHHSAWHSMHNVICGGAKLT 369
           HVGRCACF ICRNHRIWEWILRRINPR NI+FRVIDLTKHTHHSAWHSMHNVICGGAKL 
Sbjct: 342 HVGRCACFFICRNHRIWEWILRRINPRSNIDFRVIDLTKHTHHSAWHSMHNVICGGAKLM 401

Query: 370 DGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQTIILGRERE 429
           DGYLDELSRAGVKIYIYHGDQDVVAP+ECSYNLKKKAVDATVN VKNADHQTIILGRERE
Sbjct: 402 DGYLDELSRAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVKNADHQTIILGRERE 461

Query: 430 LTQELEYIWANTVDIERS 448
           LTQ+LEYIWANT DIER+
Sbjct: 462 LTQDLEYIWANTADIERA 478

BLAST of Carg03533 vs. ExPASy TrEMBL
Match: A0A6J1J6B6 (probable lysophospholipase BODYGUARD 4 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481627 PE=4 SV=1)

HSP 1 Score: 852.8 bits (2202), Expect = 6.4e-244
Identity = 414/438 (94.52%), Postives = 423/438 (96.58%), Query Frame = 0

Query: 10  SMSELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFIDAILCVIYRYIDQFLEGK 69
           +MSELTFL MDSSI S KWLIRFRTSLLDFVNFTVFLFLDF+DAILCVIYRYIDQFLEGK
Sbjct: 42  AMSELTFLFMDSSILSGKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYRYIDQFLEGK 101

Query: 70  TTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSEKMNSGSAWN 129
           TTACYCGSRGEERGNADGDGENELSETLYGRR+VFRRIALVGFSGSCKDSEKMNSGSAWN
Sbjct: 102 TTACYCGSRGEERGNADGDGENELSETLYGRRNVFRRIALVGFSGSCKDSEKMNSGSAWN 161

Query: 130 RWSDCGCSSCVDGKENRNQKLYVAVREPPRGRSEKPKENVIFLHGFVSSSSLWTESVFPN 189
           RWSDCGCSSCVDG ENRNQKLYVAVREPPRGRS KPKENVIFLHGFVSSSSLWTESVFPN
Sbjct: 162 RWSDCGCSSCVDGMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSSLWTESVFPN 221

Query: 190 LSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMG 249
           LSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKI ESVIHQFGLNSFHLVAHSMG
Sbjct: 222 LSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKI-ESVIHQFGLNSFHLVAHSMG 281

Query: 250 CLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLFGSSVMSWYE 309
           CLIALALAAKYSNSVK+ITLVAPPYFPSK G AM VLENL AKR+WPPLLFGSSVMSWYE
Sbjct: 282 CLIALALAAKYSNSVKTITLVAPPYFPSKHGVAMAVLENLAAKRIWPPLLFGSSVMSWYE 341

Query: 310 HVGRCACFVICRNHRIWEWILRRINPRRNINFRVIDLTKHTHHSAWHSMHNVICGGAKLT 369
           HVGRCACF ICRNHRIWEWILRRINPR NI+FRVIDLTKHTHHSAWHSMHNVICGGAKL 
Sbjct: 342 HVGRCACFFICRNHRIWEWILRRINPRSNIDFRVIDLTKHTHHSAWHSMHNVICGGAKLM 401

Query: 370 DGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQTIILGRERE 429
           DGYLDELSRAGVKIYIYHGDQDVVAP+ECSYNLKKKAVDATVN VKNADHQTIILGRERE
Sbjct: 402 DGYLDELSRAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVKNADHQTIILGRERE 461

Query: 430 LTQELEYIWANTVDIERS 448
           LTQ+LEYIWANT DIER+
Sbjct: 462 LTQDLEYIWANTADIERA 478

BLAST of Carg03533 vs. ExPASy TrEMBL
Match: A0A6J1J5V9 (probable lysophospholipase BODYGUARD 4 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111481627 PE=4 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 2.5e-240
Identity = 411/438 (93.84%), Postives = 419/438 (95.66%), Query Frame = 0

Query: 10  SMSELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFIDAILCVIYRYIDQFLEGK 69
           +MSELTFL MDSSI S KWLIRFRTSLLDFVNFTVFLFLDF+DAILCVIYRYIDQFLEGK
Sbjct: 42  AMSELTFLFMDSSILSGKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYRYIDQFLEGK 101

Query: 70  TTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSEKMNSGSAWN 129
           TTACYCGSRGEERGNADGDGENELSETLYGRR+VFRRIALVGFSGSCKDSEKMNSGSAWN
Sbjct: 102 TTACYCGSRGEERGNADGDGENELSETLYGRRNVFRRIALVGFSGSCKDSEKMNSGSAWN 161

Query: 130 RWSDCGCSSCVDGKENRNQKLYVAVREPPRGRSEKPKENVIFLHGFVSSSSLWTESVFPN 189
           RWSDCGCSSCVDG ENRNQKLYVAVREPPRGRS KPKENVIFLHGFVSSSSLWTESVFPN
Sbjct: 162 RWSDCGCSSCVDGMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSSLWTESVFPN 221

Query: 190 LSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMG 249
           LSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKI ESVIHQFGLNSFHLVAHSMG
Sbjct: 222 LSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKI-ESVIHQFGLNSFHLVAHSMG 281

Query: 250 CLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLFGSSVMSWYE 309
           CLIALALAAKYSNSVK+ITLVAPPYFPSK G AM VLENL AKR+WPPLLFGSSVMSWYE
Sbjct: 282 CLIALALAAKYSNSVKTITLVAPPYFPSKHGVAMAVLENLAAKRIWPPLLFGSSVMSWYE 341

Query: 310 HVGRCACFVICRNHRIWEWILRRINPRRNINFRVIDLTKHTHHSAWHSMHNVICGGAKLT 369
           HVGRCACF ICRNHRIWEWILRRINPR     RVIDLTKHTHHSAWHSMHNVICGGAKL 
Sbjct: 342 HVGRCACFFICRNHRIWEWILRRINPR-----RVIDLTKHTHHSAWHSMHNVICGGAKLM 401

Query: 370 DGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQTIILGRERE 429
           DGYLDELSRAGVKIYIYHGDQDVVAP+ECSYNLKKKAVDATVN VKNADHQTIILGRERE
Sbjct: 402 DGYLDELSRAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVKNADHQTIILGRERE 461

Query: 430 LTQELEYIWANTVDIERS 448
           LTQ+LEYIWANT DIER+
Sbjct: 462 LTQDLEYIWANTADIERA 473

BLAST of Carg03533 vs. TAIR 10
Match: AT5G17780.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 443.7 bits (1140), Expect = 1.7e-124
Identity = 214/421 (50.83%), Postives = 295/421 (70.07%), Query Frame = 0

Query: 31  RFRTSLLDFVNFTVFLFLDFIDAILCVIYRYIDQFLEGKTTACYCGSRGEERGNADGDGE 90
           +F T++   ++F VF FLD +DAILCV+Y ++D+ LE  +T CYC +   +    D   E
Sbjct: 6   KFGTAIHAALSFIVFFFLDLLDAILCVVYEFVDEILEENSTGCYCTAAAPQSQTTD---E 65

Query: 91  NEL-SETLYGRRSVFRRIALVGFSGSCKD--SEKMNSG----SAWNRWSDCGCSSCVDGK 150
           NEL SETL+GRR++FR +  +GF+   K   S K+        + NRWSDCGC SC    
Sbjct: 66  NELSSETLFGRRNIFRGMWFLGFAREFKSKLSRKLRKSKIHQESVNRWSDCGCKSCKSWT 125

Query: 151 ENRNQKLYVAVREPPRGRSE----KPKENVIFLHGFVSSSSLWTESVFPNLSETQKLNYR 210
           +N +  L+V V++     +E    +P ENVIF+HGF+ SS  WTE+VF ++   QK +YR
Sbjct: 126 KNEDGNLHVVVKDSTSRETEYSVQEPSENVIFIHGFMGSSHFWTETVFEHI---QKDDYR 185

Query: 211 LFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMGCLIALALAAK 270
           L A+DLLGFG SPKPRD  YT+KDH++ IE SVI  + L+SFH+VAHSMGCLIALALAAK
Sbjct: 186 LLAIDLLGFGESPKPRDSLYTLKDHVDTIERSVIKPYQLDSFHVVAHSMGCLIALALAAK 245

Query: 271 YSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACFVI 330
           +SN VKS+TLVAPPYFPS   G  +VL  +  KR+WPPL FG++VMSWYEH+GRC CF+I
Sbjct: 246 HSNIVKSVTLVAPPYFPSSVEG--SVLNRIARKRLWPPLAFGTAVMSWYEHIGRCVCFII 305

Query: 331 CRNHRIWEWILRRINPRRNINFRVIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDELSRA 390
           C++H+IWEW+++    +R I++++ D+T+HTHHSAWHSMHNVICGG+K+ D +L+ L ++
Sbjct: 306 CKHHKIWEWLIKLCIGKREIHWKIKDITRHTHHSAWHSMHNVICGGSKVADEHLETLIKS 365

Query: 391 GVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQTIILGRERELTQELEYIWA 441
           GVKI++  GD D + P  CS N+K+      V+ +  ADH ++I GR  E  ++LE IW 
Sbjct: 366 GVKIHLMQGDCDQIVPSHCSGNMKRTFPAVEVDIITGADHDSMISGRGEEFAEKLESIWC 418

BLAST of Carg03533 vs. TAIR 10
Match: AT5G17780.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 442.2 bits (1136), Expect = 5.0e-124
Identity = 213/419 (50.84%), Postives = 294/419 (70.17%), Query Frame = 0

Query: 31  RFRTSLLDFVNFTVFLFLDFIDAILCVIYRYIDQFLEGKTTACYCGSRGEERGNADGDGE 90
           +F T++   ++F VF FLD +DAILCV+Y ++D+ LE  +T CYC +   +    D   E
Sbjct: 6   KFGTAIHAALSFIVFFFLDLLDAILCVVYEFVDEILEENSTGCYCTAAAPQSQTTD---E 65

Query: 91  NEL-SETLYGRRSVFRRIALVGFSGSCKD--SEKMNSG----SAWNRWSDCGCSSCVDGK 150
           NEL SETL+GRR++FR +  +GF+   K   S K+        + NRWSDCGC SC    
Sbjct: 66  NELSSETLFGRRNIFRGMWFLGFAREFKSKLSRKLRKSKIHQESVNRWSDCGCKSCKSWT 125

Query: 151 ENRNQKLYVAVREPPRGR--SEKPKENVIFLHGFVSSSSLWTESVFPNLSETQKLNYRLF 210
           +N +  L+V V++        ++P ENVIF+HGF+ SS  WTE+VF ++   QK +YRL 
Sbjct: 126 KNEDGNLHVVVKDSTSREYSVQEPSENVIFIHGFMGSSHFWTETVFEHI---QKDDYRLL 185

Query: 211 AVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMGCLIALALAAKYS 270
           A+DLLGFG SPKPRD  YT+KDH++ IE SVI  + L+SFH+VAHSMGCLIALALAAK+S
Sbjct: 186 AIDLLGFGESPKPRDSLYTLKDHVDTIERSVIKPYQLDSFHVVAHSMGCLIALALAAKHS 245

Query: 271 NSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACFVICR 330
           N VKS+TLVAPPYFPS   G  +VL  +  KR+WPPL FG++VMSWYEH+GRC CF+IC+
Sbjct: 246 NIVKSVTLVAPPYFPSSVEG--SVLNRIARKRLWPPLAFGTAVMSWYEHIGRCVCFIICK 305

Query: 331 NHRIWEWILRRINPRRNINFRVIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDELSRAGV 390
           +H+IWEW+++    +R I++++ D+T+HTHHSAWHSMHNVICGG+K+ D +L+ L ++GV
Sbjct: 306 HHKIWEWLIKLCIGKREIHWKIKDITRHTHHSAWHSMHNVICGGSKVADEHLETLIKSGV 365

Query: 391 KIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQTIILGRERELTQELEYIWAN 441
           KI++  GD D + P  CS N+K+      V+ +  ADH ++I GR  E  ++LE IW +
Sbjct: 366 KIHLMQGDCDQIVPSHCSGNMKRTFPAVEVDIITGADHDSMISGRGEEFAEKLESIWCS 416

BLAST of Carg03533 vs. TAIR 10
Match: AT5G17720.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 379.4 bits (973), Expect = 4.0e-105
Identity = 195/424 (45.99%), Postives = 266/424 (62.74%), Query Frame = 0

Query: 41  NFTVFLFLDFIDAILCVIYRYIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGR 100
           ++ VF   D +D  LC+++R++D+ +E K+ +C+C +  E+   A+ +    LSETLY R
Sbjct: 19  SWAVFFLFDLLDYFLCIVFRFLDEVMEEKSESCHCNNPREKTHFAEYE---FLSETLYRR 78

Query: 101 RSVFRRIALVGFSGSCKDSEK----------------MN--SGSAWNRWSDCGCSSCVDG 160
           R+VFR+   + F+    +  K                MN  S    NRWSDCGC +CV  
Sbjct: 79  RNVFRQAGFLRFANKLPEITKKIGIVTFLRKFLFPHTMNKVSHEVANRWSDCGCKTCVSW 138

Query: 161 KENRNQKLYVAVREP---PRGRSEKPKENVIFLHGFVSSSSLWTESVFPNLSE-TQKLNY 220
                 KL V V++P       S KP ENVIF+HGF++SSS WT +VF  L E T+  NY
Sbjct: 139 --INTDKLNVIVKQPSISDLSISNKPVENVIFVHGFLASSSFWTNTVFKYLPETTEGTNY 198

Query: 221 RLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMGCLIALALAA 280
           R FA+DLLGFG SPKPR   Y++K+H+E IE+SVI    L SFH+VAHSMGC+I +ALAA
Sbjct: 199 RFFAIDLLGFGDSPKPRASQYSLKEHVEMIEKSVILPNNLTSFHVVAHSMGCIIGIALAA 258

Query: 281 KYSNSVKSITLVAPPYF-PSKDGGAMTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACF 340
           K+S+SVKS+ LVAPPYF  SK G +   L+ +  K++WPP  F +++M WYEH+GR  C 
Sbjct: 259 KFSDSVKSVALVAPPYFADSKGGASCAALDVVAKKKLWPPASFFTAMMCWYEHIGRGVCL 318

Query: 341 VICRNHRIWEWILRRINPRRNINFRVIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDELS 400
           V CR+HR WE I++ +  RR +   ++D TKHTH S WHSMHNVICGGAK TD +L+ L 
Sbjct: 319 VFCRHHRTWERIIKIVTWRRKLPTAIMDFTKHTHQSGWHSMHNVICGGAKFTDKHLETLI 378

Query: 401 RAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQTIILGRERELTQELEYI 442
           ++GVKI +  GD+DVV PI+C  N+K K     V  +   DH T+I+ R       L  +
Sbjct: 379 KSGVKINVMQGDKDVVVPIDCLSNMKGKFPAVEVEVIAGTDHSTVIMSRREVFAANLVSL 437

BLAST of Carg03533 vs. TAIR 10
Match: AT1G64670.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 350.1 bits (897), Expect = 2.6e-96
Identity = 178/458 (38.86%), Postives = 264/458 (57.64%), Query Frame = 0

Query: 32  FRTSLLDFVNFTVFLFLDFIDAILCVIYRYIDQFLEGKTTACYC------------GSRG 91
           F  SL   V   VF  LD +D +LC  Y+ +D F E +   CYC            G+RG
Sbjct: 3   FSRSLNRTVGVFVFFILDIVDFLLCFTYKTLDFFFESEWKPCYCCPPPEAKPISAGGNRG 62

Query: 92  EERGNADGDGE--------------NELSETLYGRRSVFRRIALV------GFSGSCKDS 151
            +   ++  G+              +E+S+TLY R S+  ++  +           C +S
Sbjct: 63  GKMIVSERSGDYSKVVSLTRTKIYLDEISDTLYSRPSLLTKLTKLVKCFKKDVVKCCDES 122

Query: 152 EKMNSGSAWN----------------RWSDCGCSSCVDGKENRNQKLYVAVREPPRGRSE 211
           +K +  +                   RWSDC C+ C     + NQ L+V V++P      
Sbjct: 123 KKRSPSTKKTLLTVNSTVVEKLQRTPRWSDCHCTFCTSWLSSSNQSLFVNVQQP---TDN 182

Query: 212 KPKENVIFLHGFVSSSSLWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKD 271
           K +ENV+F+HGF+SSS+ WTE++FPN S++ K NYR  AVDLLG+G+SPKP D  YT+K+
Sbjct: 183 KAQENVVFIHGFLSSSTFWTETLFPNFSDSAKSNYRFLAVDLLGYGKSPKPNDSLYTLKE 242

Query: 272 HLEKIEESVIHQFGLNSFHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDG--G 331
           HLE IE SVI QF L +FHLVAHS+GC++ALALA K+  ++KS+TL+APPY+    G  G
Sbjct: 243 HLEMIERSVISQFRLKTFHLVAHSLGCILALALAVKHPGAIKSLTLLAPPYYSVPKGVQG 302

Query: 332 AMTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNINF 391
              V+  L  K VWPP+ FG+SV SWYEH+ R    V+C+NH + E++ R +   R   +
Sbjct: 303 TQYVMRRLAPKEVWPPMAFGASVASWYEHISRTVSLVLCKNHHLLEFLTRLLTRNRMRTY 362

Query: 392 RVIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDEL-SRAGVKIYIYHGDQDVVAPIECSY 439
            +     HTH+++WH++HN+I G     + YLD +      ++ ++HG +D + P+ECSY
Sbjct: 363 LIEGFLCHTHNASWHTLHNIIFGSGSKVEAYLDHVRDNVDCEVAVFHGGRDELIPVECSY 422

BLAST of Carg03533 vs. TAIR 10
Match: AT5G41900.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 336.3 bits (861), Expect = 3.8e-92
Identity = 178/460 (38.70%), Postives = 266/460 (57.83%), Query Frame = 0

Query: 40  VNFTVFLFLDFIDAILCVIYRYIDQFLEGKTTACYCGSRGEERGNAD--------GDGE- 99
           V F VF  LD  D +LC  Y+ +D FLE +   CYC S  E +   +        G  + 
Sbjct: 11  VGFFVFALLDIADFLLCYTYKTLDYFLESERKPCYCSSPPEAKAKTEKIIVSERGGYSKV 70

Query: 100 ----------NELSETLYGR--------RSVFRRIALVGFSG---------SC---KDSE 159
                     +E+S+TLY R          + R +    + G         SC   +  +
Sbjct: 71  VSLTRSKIHFDEISDTLYSRGPSLLTRLSKLVRSVKCFNYKGLIMRGNVVESCDHHESKK 130

Query: 160 KMNSG---------------SAWNRWSDCGCSSCVDGKENRNQ-KLYVAVREPPRGRSEK 219
           K++ G               S   RWSDC CS C     + N+  L+V V++P    ++K
Sbjct: 131 KISKGKKRLMTLNSTVIEKSSTAPRWSDCHCSFCTSWLTSTNRDSLFVKVQQPK--DNKK 190

Query: 220 PKENVIFLHGFVSSSSLWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDH 279
            ++NV+F+HGFVSSS+ WTE++FPN S++ K NYR  AVDLLG+GRSPKP D  YT+++H
Sbjct: 191 ARDNVVFIHGFVSSSAFWTETLFPNFSDSAKSNYRFIAVDLLGYGRSPKPNDSLYTLREH 250

Query: 280 LEKIEESVIHQFGLNSFHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDG--GA 339
           LE IE+SVI +F L +FH+VAHS+GC++ALALA K+  ++KS+TL+APPY+    G   A
Sbjct: 251 LEMIEKSVISKFKLKTFHIVAHSLGCILALALAVKHPGAIKSLTLLAPPYYKVPKGVQPA 310

Query: 340 MTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNINFR 399
             V+  +  K VWPP+ FG+SV+SWYEH+GR    V+ +NH++ E++ R +   R   + 
Sbjct: 311 QYVMREVARKEVWPPMQFGASVLSWYEHLGRTIGLVLIKNHQLIEFVTRLLTLNRMRTYL 370

Query: 400 VIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDEL-SRAGVKIYIYHGDQDVVAPIECSYN 442
           +     HTH+ ++H++HN+I G     D YLD +       + I+HG +D + P+ECSY+
Sbjct: 371 IEGFLCHTHNGSFHTLHNIIFGSGAKLDSYLDHVRDHVDCDVAIFHGGKDELIPVECSYS 430