Carg03529 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg03529
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptioninsulin-degrading enzyme-like 1, peroxisomal
LocationCarg_Chr14: 2181339 .. 2203098 (+)
RNA-Seq ExpressionCarg03529
SyntenyCarg03529
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAATCCATTTGCTCTCAGGTTACGATTTTTATCGTTTCAAGCAAAATTTGTCCAAAGACTTCGTATAATCACCGATCAAGGTGCTGAGTTTTCGATTCAATACTCTCCTTCTCTGTGTAAGTAATTGCAGATACGTTCTTAGTCTCTAATTTTGTTGAATGATTTCACTTTTTTCGCTCAAATTTACTCATCTTGTGTTACTGGATTTCTGTGTTCGTTGAAGTGGGGATAGAAGCAATGGCGGTTGGTAAGGAACAGGTGACTCATGACATAGTGAAGCCTCGTACTGACAAAAGAGAGTACAGAAGAATCGTCCTCAGGAACTCCCTTGAGGTTCTGCTAGTCAGTGATCCTGATACCGACAAGGCAAAGCCGACTTTCACTTTTGTTATTTGTTTTTTTTTTCTTTTGTTTTTTTTTTCAATCTGGTTATTGTTTTGTTTTTTGTTCTTATCTTGTGCATCCGAGTGAAGTTGCTCAAATTAGGGCTCTGTGTTCTTGAATTCATGTGTCTTTATCTGTTTGAGTTTCAGAGTGCTGCTTCCATGACTGTTAACGTTGGTTCCTTCAGCGACCCCGAAGGCCTAGAGGGTTTAGCCCATTTTCTTGGTAAGAACTTGGTTGTGTATGTGTGTGTGTGTGTGTGTTTATTATTTATTTATTTTTATAATCCATTGGATTCACTTTGTAACTTAAATCCACTTATCTTCATTTAGGCCTTCATTGGTGATGTTTACGCCTCTCGTTCTCTGAACCTTTGCCAGCTTGTGTATTTTTTTAACAATTAATTATTTAAAACACGGTAAAACATTTGAGAGATTAGATAGACTCAAAAAAGAAAAAAGAAAGAAAGAAAAAAAGAGACATTTGATAAGATGAACCGTTGGTTTGAAAGTCATGACTAAAAACAGTTTCTGAAGCAAATGTTCTTTTCTGAGACGATACTATTGAAAAGCAGTTTTTATTGATGCATTTCAAGTTATTTGTTCAATCATGAATTATTTTCTGAAGTATATTTTGTTTTAAATGTGTGATATTTTAAATTAATATGTAGAGTAAAGAAATGAAAAGAAAATAAGTAAATATATCTTTAAATATTGTTTTAAATTAACAAAGTAGAAATAATGCTGGCAGTTTTAAGAAACAACTTTTAGAAAATTCGCCTACTGACATGTAGAGGAAGAGCCGCATAAGGGAGGTTTATGATTGGAGTTGCTGGGGTGTGTTTTGTAGTAGTACGCCAATTTTCTTTATTTTGGATTCTTTTTAATGAACTTATATACGATTTTTTTGGCAGAGCATATGCTGTTTTATGCAAGTGAAAAGTATCCATTGGAGGATAGTTACTCGAAATATATCACAGAGGTATCTAAATTACTGTTTACAGAAATGCATTTGGCTAATTGCACATTCATTCATGCATGTGAAATTAATGGGATGCTGATGGCATGGTTAATTTGCTCTTATTTGGTTCATCAGTTCTTCTCTAATGTAGATAATCCCAGCATGAAGTTTAGGACATCAAAAGCATGAAGTTGTGATATCTAGACATACATGTGGTTCAATAGAACTTGGAGTGTACAATGAAAATGTACAACTCTGTCTTTTCTCTGTTTGTTTTGCTATAGAAGAGTTTTTATGAACAAATTTGAAGATATTGTCTATGATTTCTTTGTTTCAGCTCCTTTTTGTTTGGGCAAACTTCTTTTGCATTCCCTGGTCGCATATCCTTTCATTTTCTTTATGAAAGTTTTTAAATCAGTTAATTTATTTTGAAAAATTAAACATTCATATATATATATATATATAAGGGTATATAAAAACGCCAAGCCCACGACAAAAAAGCCTACAAAAGGGATCCAAACGATAGAAAGAGGTTCCAATTGAGTAAGATAAGACCCAAAGGATAATTAAAAAGAGATTTCTCATTGCAGCCCAGATATAGAGCCTAAAAAAAGAACAAACATCATGAAATTCTTGACCCTTCTAAAGGTCCTATGATTCCCCTCTCAAAGGCTCTAAAGGTCCTTGAAAAAATTCCTACAATGATAATCAGTTTGCAAGAAGTGGAAGCTATGCTGATTTGTTGGGCTGTGAGGTTATTATAGGGCCTCTTCCTTTTGGGAGCCAATGGTCGAAAAGTTCAGAAAGATTGGATTTTTCAAAGGGGGCTAGACTAACTCTCGCCCAATCAGTGCTAAGCAGTGTATCTCTCATCTACTGCAAAAGGTTCCAAGAAAGATCACCAAATTGATAGGCTGGTTCAAAATTTCTTCTGCAATTGTTGTGACTCCAAAGCTTGCAACCATTTAGTAAAATGGAAATGGACTTCTCTCCCACTTTTCTATGGAGGTTTGGGAATAGGCTTGTACTTACGAAGGAAGAATGCCCTGCTCCTCAAATGGCTTTGGAGATCTACTTTGGCATCATCTAGAAGAAATTGATCATAAGCTTCTATGCTTTGACCCCTTTGGGTGGATTATCATGGTGGGATGTAACTAAAAGTGTGCCTTTTAAAAAAAAATTCTTGAAGTTTAAGGCTTTGAAAGGAGATAGAATCATGGAATGTGGTCAACAAATTCTGGGACCTGAAGCCTGGTAGGAATTTAAAGGATAATGAAGCAATGGAATGGGCCGAATTAAGCTTTGACCTTACCCCTGTGGTATTGTCAAACAAATCATACTCACAGACTTGGCTCGCCAGTGCCGATGGGGTCTTTTCTACATAATCCTTGATGATGGACATAGAGGAAAAAGTAGAAGCAATAAATCCCACGCTTGCAAAGATAGTATGGAAAGGACATCAGCCTAAAAAGGTGAATTTCTTCCTTCGGGAAAGAGCGCATAAAGCCATTAGCACAAGTGAAAATCTTCAAAAAAGAATGCCTTACATCACCCTTTCTCCAAATTGGTGTCCATTGTGCAAGAAAGAAAACGAATCACAAAACCACTTATTCATGCAATGCACATACGCTCAGAATTTTTGAATAACGATTCTCAATATATTTGGCTGACATCTCACATTTCCTAGGGAGGTAAAGGATCTACTGGATATGACCTTAACGTACCACCCTTTCAAGAATGCAAAAGCCCTACTATAGGAACTTCATCATGGCTTTCTTTTGGAATCTGTGGAAAGAAAGAAATCAGATAATATTTGCAGAAAAGACATCGACTTATTCAAAACTTTTCGACAGTATTGTTTACCAAGTTATATCTTGGTGCAAACTGTCTAAGGTCTTTTGTAAACATCGTGAATATTATCCTTTTGTAAACTTCAATCATAAATGAAGTTGTCTCTTATCGAAAGAAAAAAAAAAAGAAAAAAGAAAAAAAGGGAAATAGAATCAGATTTTGGACTGAGATTTGGGTGGGAGAAAGCACCCCGAAAGATCCTGTTCCCTGGTATATATATGGTTTCAAACCCAAAAAATTCAACTATTGCTAAGTGCTGGAATGAAAGACATTTTGATTGGGACCATAGCTTCAAAGAAGGCTGAATAGTTGGATGCGTTTGGTAGAAACTTTAGATTCAGTGCAGCTTCAATCGGACAAGGACAGTGTGAGATGGACCTTGGTGAAACTTACTGAAACAAGGCCTATCATTAAGTCCCCTCTTATCAATCTGATCTGGAGAGATAAAATTCCTTAAAAGATTAAGGTTTTCCTATGGTCCTTGCTCACAGAAGCCTTAACATCTGATAAGCTACAAAGGAATTGTCGAAAATTGACTTGTCTCTCTCCATGTGCTGCTTATGTTCTCAGGGTGAAGATTCGTTGGACCTTTTATTTTTGAACTGTACGTTTATGAGTAAAAAGATGAAGCTTTTTCTTCAATATCTTTGGCTTGTCTTTATGTCTTCCTCAGCGTATAGAAGTATGGTTTTTGGAAGCCCTGGATGGTTGTGGCCTTAAAGGAAGAGCTAGAGCTTTAGGGAGCATTGGTGTTAAATCTCTTTCGTGGTGCTGCTGGAAAGAAAAAAACCAGCCGAATTTTGAAAGAAACGAACCAGCCGAATTTTGAACAGGAGTTTGAGCATTTTGATAGTTTTTTCTCAGTGTTCAGCTCTTTGCTTCTTGGTGGTGCTATAGAAATAGTAAATTCTTTTGTAATTATTCTATTTTTTCGGTTCTTTAAGATTGGAAAGCTCTTTTGTGATTAGTTTGCTAGGGAGGGGTCTCTCTTCCCCCTGCCCTTTAGGTGTATCTCCCCTCTTTTTTTGTTCATAATACAGATTCCAGGTTTCTTATCAAAATATATCTTTGGATAATTGAAAACAACTTAATTCTCTTGTTTCAGTCTTTTTATTTATTTATTTATGAGTTGGGGATCCAATATGTTGCTTTTCTATAAAATGGCTACTTATTAAATTTCTCTTCATATTTACTGATTAGTTGCATCATGTACCTGTGAAACTAACGTTTTCTATATCTGGACAAGGAAAGAAGTCCTGTTTAGATTGTCAACCTTGCATTACAGTATCAAATATTAATCTTTTTTTTTTTAGGTCAGAAGTAGATTTAAAAATGTTTTAATGTGAAAGATGTTGGGTTGACATTTCTTGCATATTTATATGTTTGGAACTGAATAAAATTCTCCAATGTTTTGAACAAGAAACAAAGCCTCTCATTAATGATTGAAGTTATTAGAGAGCACAAGAAAGTAGAGTATACAAAGTGTAGACTAAGAAATAGAAACAAAGGAGTACAGGAAAAAAAATGTAGCACCCCAAGATTCTGAAATTCTACAAGCAAATCCTCCAATTGTTACAAATGGCGTTGGAAGAATAACCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAGAATAGACGAATAACACCATTTTGAAGCTTCATTTTGATGAGCTCAAGAGGATCCTAAATTGATTTGGCTTTAAAGATTTTTTGATTGCTTTCCAACCAAATATTCAAATCCACACGTTGGAGGCAGTATTTGAAATAGTGTAAACGATCAAATTAACTCCTACTTAATGGCTAAAATTTTTGAGATGATGGTAATTTTTTTAGATGGGAAATGATTTTATTGAAATTAATGGTAATTTAACTATTGACTGTATAGTTGTAGCTCTAGAACGTAATAAGTCCTCTATTTATTTTTTAAGGCTTATTTTAAAAATAATATTGAGATCCCTATTGATTTGAATGTTACAATTTCTCTATTTTCCTATTCTGTTTTTCTTTTAATTGTCATCAATAGTTGGTTGAACAATACTTGAGGTCCCTAATTTTTTTAGTAGAAAAAGTATGATTTAGTTCCTAAATTTTAGTTTATATCAATTTGGTACATCTACTTTCATTTCTGTAGCAGTCTATAAGCAAGTATATAACAATTTAGTCTTTGTTCATTAGAACTCCTATCGATCAAATTGAAGTTTCAGTTTTTTATATAATGACTTAATCTTTATAGTTTATGAACACATGTGGTTCCTAATAATTTTACAAGCTACATATGAAAGAAATCTAATGAAATATCAATAAATATTTTTCACCATAGGAACTACATCTTACAAATTTGAAAGTACAAGGTCTAAATAAACATGTTACGTATTAAAGTTCAGAGACTATATTGTTACAAAATTGTGAGTATACAGGGACTACCTTCACAAAAGTTCAGGGCAAAAAATGTTTTATTAACCTTAGTTGTTTTCTTTTTGTCAAATTACTCTCTCAATTCTTCATGTGCATGCTTTGTTGAATATGTTTTTATGTTTTTTCTGTTGACATATAGCATGGAGGTAGCATGAATGCCTTTACGGCATCTGAGCAGACAAATTATTATTTTGAGGTCAATGTTGATTGTTTTGAGGAGGCTTTGGACAGGTATATGGTATTTCACTTCTTCTCGTTGCCTGCACAGTGTTGTCTGATTATGATTTACCTTTTTCTACTTCCAGATTTGCTCAATTTTTCATCAAACCTTTAATGTCGCCTGATGCTACAATGAGAGAAATCAAAGCGGTTGATTCTGGTAATACATGTGTACATCATATTTTAATTTGGTGGTTGTTTTTTTATTTCCTTCTTTCGGGTAGGTAGTTTCCTTGAACATCATCTATCTACAATAAGATATATATATATATATATATATATATATATATATATATATATATTTCTTTTGTCTTTTTGCTTAAAAAAAACCCAATTTGATATTTTGGGTTTTGACATTTAATCTATTTGATTTGCGAGTCTTCAACCTTACAATCAAACACTTGATGTTGAAAATGTATAAATGGTCCATGCAGCTGAAAACATATTAGATTCGGTTTTGCTGGCATGCATAAAAATTGATGAATGGGGTTCCAGTTAAAAGGAAGAAAAAGACTGGACCTTTTTTTATTTTAAATTTTGTAATTAATTTAATAATTTAACAAACTAACAAAGTAATAAAGTAAAAACTGGGTTTAAATTGTGAGCTTTGGAAACTCAGTGTCCCAACAGACGAAAGTAAAGTATTAGGGGTCGTAAATCTTATTTTCTCTATAATTTATATTATGAGTAACTTTTACATTTGTTTTTTTATGTGTGGCCTCTTCTACTCTCATCTTTCTGAATGGAAGCTTCATTTAATTGCAGAGAATCAAAAGAATTTATTGTCTGATGGCTGGAGAATGCACCAGGTAATTATTTCTCCCATGATTTCTTATTTGGATGTACAATAAATGCAAGATATATACCATTACTAGTATTATTGAGATTAGAGACTGGTGAAATGAAAAATAAATGTGTACTCAGATTAACCCAACAAGAATTTGAAACAAATAAATATATCCAAAATAAAAATCTATTTTCACAAAAATAGGAATAAAATGATAAAAATACATAATAACAAGACATAGATCAATATTTTATGCCTAGTTCATTAGTGTAATAAAATACATAATATGTTACATATGCAGCTTGATTATTTGATGAGCCGATATTAAAGTTTATATCTTCCATTATGGATTAACTGTTATTTATCCCCTCAATTTTATAGAGTAGATGCATCGTAATTCAAAAGAGGTTCCTCTACTGGGGATCCCATTTCTGTACCAACTGAAGATATGTTTATTGGACATTATGTATAACACTTCTTTAAAGGTAAGGATTAATGCAAATTGGAGAAAATTGCAAAAATCAAATAGATAGTAGTGAGTTTTCATTGAATATGAGCGACTCATCCGTTCCTTTCCTCTATTTTTCTCTACTTATTTAGAAGGAATTACATGATAATTTTTTTTTTCCTATATTGGTGGGATTTTTGGAGAAGAATGATTTTCTTATGTGATTTTGAGTTGATTTTAAATAAAATTAAGAAAAAATATCTATAAATCAAATGGAGGGTTTAAATTGATACAATTATTAGTTTGAGGTTTTAATTGGTACGACCTCCAAAGTTTAGAGACATTTTTTTTTTTTTTGAAAAAATTAAATGAAAGCCAACTTTCATCAAGAAGAATCAAAGTAAAGCTGACTTGGAAGTAGATTTATTTATTTATTTATTTTTTTGGTTCATATTTGTATATATATTTTTTTAATGATAAGTTAACTTAAGGATGAAAAAGAAAAATACAAAAGTGGTCTCAAGAAATCCAAATCAAAAGTGGCTAGTACAAAAACTTATTATGGTTTAAAAAATGAAGGAGAATTACAAAACAAATTAGAGTTAAGAGCCCACGGAGAGGTAATAAAATATGCTGTATATCAAATATCGTCATAAATATATAATATAAATGGAGAAGGAAATCTCCGGTTGTCTAAGAAAAATAAATTGATGAGCCTATATTTGGTGAAATTATTTCCAAATTTAGGGTGGGTTTAACCAACAAATTCCATTCATGCTAGTTAAGAACAAAAACAGAAAAAGAAAAAAAATGGAAAGCACTGTGCTCCACTTGAGTCACTTGTATAACGTAAACACTTGGCCATATAATCCCTAACCCCAACCTTGCCTTGGCTTATAACTAAGTCCTCGCTTGTAAATCATCACAATCCATGCTCAAGTACTGGATTGTTTCACACTTAATTTTCTATAACTTTCTTTGGTTAATTCATGCATAAGGCATCATAAGTATTGCAGAATGTTTCTGTTTTTATTTTTGTTCATTGTCGCAATCTATGCTTGACTGATCATGACACCAAGCATGACTTCTCTACAAATTTCTTCTCCTTAGTCTCTTACAAACCATAAAGAACCCTTTTTTTTGTTGTGAAAGACCTATATTATAACTGTGTAGCTTGACTATGTTCCGTATGAACATGAAAAATAATTGAGGAAATATCCTGTACAGCTTTGCAGAAATATGGAAACAAATTGATAGAATGGTTAAGGTAGAGAGGGAATTTTCCAGAACCGTAGGATTTTCAACCGTATAAATGTTACCTTAATAGTTTTCTAATCATAATACTTAATAGTATTATAGCTTTTGGAAGAAAATTCAATGTATTCACTAAAATTCATATTACAAGAAGATAATACAAAAGAAACTTCAGAAAAGGTAAATAGATAAAATGAGTAAAGAAAAATAGCTAACAAATATAAGATACAATAAAATTAGTATCTAATATAAGTAAAGATAAAATATTCTGAAAATGAAATGAAATCTTTAAGAATTACCCTTTTTCTTTAGTCAGAAGTTTGGACTTGTTTATATTTAGAGAAATTTCTCCTTTCTAACCAAAAGTTATCACTTTATGCAGCTTCAGAGACATATAAGTTCTGAAAGTCATCCATTTCATAAATTCAGCACAGGTTATAACATTATATGGATTTCATAAGAGTTGGAATTTGCTTGATTTAATAAGTCGACAAGTTTTATTGCATATCTCAATATATGAATTATTCGCATGTGGACAAAGAAAGTTCAATTTATTTACTTTTGGGGCATTGGGTTTTTATCTTCGTCCTCATTAAATTGTTCAAGTTAAGAAATCTCCCTTTTAATGACATAAAAAAAATAGTTGTACTTTAACATTGACTACTTTTCTGGCAGATAATATTTCTAGAATTAGTTTAACTATTTAATGATTCCGGGGAAAGTCTTCTAGCTCATGCAATTACCTCTCGGTTTTCATATTGTTTACACTTTGATGGTGGCGTTTAGAAGGCCACAAGTATCCTTATTGTGCCAGACATTTTTACTTACACAGGGAATTGGGACACTTTAGAGGTTCAACCAAAAGCTAAAGGGCTGGATACAAGGCATGAACTTCTAAAATTTTATAAAAATAATTATTCATCCAATGTCATGCATCTGGTTGTATATGCAAAAGGTTAGTACTCATCCGGCCCATTATAAATTGGGTTTTGTACTGTGTAGAATGAAACAATAGCAACTTAAATTTCGTACTTTTACCTTATTCCAAAAACCTTGATAAAGTATGAATCCCTTATTTTACCATATTCAGAAAACCTCGATAAAGTACAAAGCCTTGTGGAAAACATATTTCAAGATATTCCAAATAATAACTGCAATCGCGCCAATTTTCCTGGTCAGCCATGCATGTCAGAACATCTTCAGGTATGTGTATTGCATCACGCCTAATGATCTGCTGACAAAGGATTATGTATGCTATGGACTTGATTTTTAATTTTGATGTGATTGCTTCCCGATGTTTATTATATGGATTTTGAAGTGGCTTCTCTCGTTATTTGGAAGGGTTTTGTTTCTTATTAAAAATTGTGACTACTGGTTACCACCGTATATTTTTTCTCTGCGTTTCAGGTTCTTGTTAGAGCCATCCCAATTAAACAAGGTCATAAATTGAGAATTGTTTGGCCAATAACTCCTGAGATTCATCATTACAAGGAAGGGCCATGCCGGTATCTGGGTCATCTTATTGGCCATGAAGGAGAAGGATCCTTGTATTATGTTCTTAAAACATTAGGTGCGTTTTTGTACATATTAACAAAAAGTTTTGGACTTTCAAGAGCGAGATTTGGACTTCAACTTTCATATATTGAGTTGTTTGTTTGATTATATTAGACGACATCATGTTGGAGTGAATATCTTCGGTTTATCTTAATTAATTCCATGTTGAACTATTATTTATTTGGGTTTCCAAAATTGGGTTTCCTTTTGTAAATTTCTGTTTTCTTTTTGGTTACCCACTCTATGTTGGTGGAGCCTTTCTGGCTGTTTGCGGTTTTTTTATTGAAAAAAAGTATGTATGCCTATATCAGTGGACTAACATGGTTAAGGTTATTTTGTGGCTGATTTGAATTTGGAAGGAAAGAGGCTATATAATTGTTAAAGATAAGTTCATCTCTTTTTTTGTTTTGGTGACTGTGTAAAGTTCATGGCCTCTAACTGGAATAGCCAACACAAGCTCTTTGCAATTACTTCACTTTCTTCATCAGCTTAGATTGAAAGGTGTTTTTGTAATTAGTTGCCTTTGGGGAGGGGCCCCTTGGTCGGCCCATCAGGCTGTCCTTTTTTTTTTTTTTTCCTTCTTGAATGAAAAGTTTCTTGTTTCTTATAAAAAAATTCATTGCCTTAATTATTATGATCGTTTTTTTAAAAAATTGATTTGATTTATATTGTGGGGGAAAATAATACAGGGTGGGCAACAGGTCTCTCAGCCGGGGAATCTGATTCCACGATGAATTTTTCCTTTTTCAAAGTAGGAATAGATCTCACTGATGTTGGTCAAGGTACTTACTCGCTTTCTCTCTCTTTCTAATAATCATCCTTAATTTAATCTGAAATTTTATTCCCTTGATGTCGTTGTAGAACACATGCAGGATGTTGTTGGGCTTCTCTTTAAATACATCAGCCTTTTGAAGGAATCTGGTATTTGCCAATGGATATTTGATGAGGTATGTGCATGCATTACTATAACCCCTTTAGCATGCTATAAGCTAATTAAAGTAATTTTGAGCAGCGTAGATTTTTGTACTTTTGTGTGTGTGTGTTTGTTAACTTTTGGAGGGCGGGGTTTTGGATTTTTTGAAACCTCTTATGGTGTTTGACATTGTAGACGCCATGAAAGAAAATATAGAGGGAACTCCCATCCACATATTTTTCCAAAATTTATTCATTTATTTCTTTTATATTTTTAAGCATACAGCTTGGCAGATGATGAGTTATTTAGTTTCTTGAAATAAGAGAATATCAACATTTTATTGTATTAAATAAAAAGAATGTGTGACATGGCCATCTTTTAATTAAAATTTCCCGTTTTTGCTTCCTATTTACAGTCCACATTTTTTATGTAGTAATTATTGTTCTTTTGATTCACAAAGATTCTGAGACAACTCTACCTTTATCAAGCTTTCAGCTATTTGTGAGACGAAGTTCCATTATCAAGATAAAATTCGTCCAATTGACTATGTAGTCAATCTTTCATTAAATATGCAGGTATTTATAATTTTTTCGAATAACCATTCGTCATAAAATTTGTGGAATGATTTTTTCTCTTTATTTCCTGTTATTTCTATAAATCATAGGAGAACAGCATGTTATTCTTTATTTTTTTTTTTGGCCTAGCCTAGGTTTGAAATTTTTGATTTGGCTGGACATTGTTCTATATTTGCGCTGTGTATTATTGTATGGTGGCCTTCGTAATTATTTGGTGAAAAGATACCAATATCAATCTTTTGAATGCTCACTGTGTCTTATCCATTCTTAAGTGTTTAAATTTTTACTCTAACCAGTTGTTTTTTTCCACTACTTCTCCTAGATTTTAAAGCTCCTTCTCTTTTGCATTACTTAATAGGCAAAATACACTTTTGGTTCTTAAAATTGAAATTGGTATCTATTTGATCATTGAGGTTTTCACAGGTACACTTTTTGTCCCTAGGTTTGAAAAATGGTTCTAAGTAGTCCTTGAGTCCATAATAACCTTTAGTTGACTAACGAAAAATGATGTGTTAGCTTTGTCCTAACTAGACTTGATAATTTGACATTATTTTATGAGTGGACAATGTAAGTTGGCATCTCATTTTAGGTAATGAAGGATGGTCCCTCGTTTAGTTTCCCTCGTTGTAGATCTGAACAAACGAAAATCATTTAGATTTTAAATGAGGGAGGTCCCTCGTTTTAGGTAATGAAGGGAGAACCTCCCTTGGCCTTCCTCTAACCTTTGTTTGAGATTTGAAACTGCTGGAGGCTTCCTCTGTGTTGAAAACTTGGCCTGTCTTCTTCTGGGTTTAATATATGAAATACATTGGAAATGATCGACAAGTTTCATTCACCAAGTGTTGTCTTTTATTGAAAAATTGCCTAGTCGTTTTGAGTATCCTGCACTTCAAACTAGCCGCCGTATACAACAAACATGGTGGCTGGAGTATCATTTTTCCTTCCATTGTTTTCTTCTCGAGTATCATTTTTAAATTGTATTTGGTTTTATGTTCATTCGAGCAAGTAATAAAATGTAGACAGATTCTGATTTGTTCGTAGTCTTATTGCCACATGATCTGCCAATTTGAACTCAGAGACCATTTCAAACTATTTTCCGAAGTTTGGAAATTAGTGTCAACCCCAAATTTCAGAGATCAAAAGAATAGTTTGCTCTTATTTAACTTTAGACGTAAACTTCAGTGAATGACCCTAAAACCACGCATGTGGGTCAGAAAGTTTCTCCCTTGTTCCTCTGGTCTGTCGTTTAGATTCCTTGTCTACATGCACTAAAGGTTTTAAAACATGGGCATAGACATGGGATACTAGCTTGACACGACATGCAGATGAAATTGTATGAACATGATCAAATTTCATTTTTTAAAAAATAAAGGCCGAATAGTCGGTACTTTGTTTAATAAATACTCTTAAACGTTCCAAAGTTTCATTATTATTAACTTTAAAAAAACTCTCAAAAATACTCTTTCCATTAGTTTGTGGATGGAAAACGTAAGTACTTTGTTTTAAAAAAATATCCTTATCGTTAGTATATAGACGGAAATTGTTTGTACCTCATTTCAAAAACAGCCATGAACCTTTCAAAGTTGCATCAATTTCCTTAACCTCACAAAAAAGAAAAAAAGAGGTTAAAAACGTCATTATTGTTAGTTTATATACGGAAACCATTTATCTATACTCCATCACTTCCTTTTTCCATTTTTTCTCTCTTCTCTACTTCAATCCATGGCCTAACATTTCTTCATTTCTCATATTTTTCCTCTCACATTTTTTATACACTTTAATCCCGTTCTTTCGTTCCTTAATCTCTAAAGCTCCTCCATTTCTCTTTGTTTTTTCCTTGCTTAAGCAATCATAAATATACTAAATTTCAACTCAACTGCTAAGTACAATCTTGTTAAACCATCAAAATCCTAACGTTCCTTTATGGTTCTATTATTCTCCGCTATAGCCTATATTTCTTGATTGAAGGAGCTAGTTTAAGAGATTGGAAAGAAGGGGAGTAAGAGGGTATAGAAGAAGGGAGGAAAAAAAAGGAAAATAGGAAAGTGTTAAGCCATAAATTGAAGTAAGAAGAGGGAGGTGATGGAGTGTAGATAAACGATTTCTGTCTATATACTAAGACAAAGAGCATTTTAAAACTTTTGTTTGGAATATTAAGGGTATTAATACAACTTTTTGAAATTTTTTTTTTGAAAGTTTAAGGGTGTATTCATAAAACTTTTGAAATTTCAGGACTGCTTTTGAAAGCAAGTACTAATAGAATCCGTAGAAAAGTATTTTTAATTTTTTAAAAAATTTAGAAGTTTCTGAAATTTCTAATAATTGAATCGAAGTTCAAAGATAAGGAGTATTTTCTATAATTTAGTCAAAAATAAAACATTGGCTATCTCTAATATTTTATTTGTACCCATTGAAGTAGCAAACATAGGCATAAACATGGGTACTAGCATGACATGACGTGCAGGTGAAATGGCATGAGCATGATCAAATTCCAATATTTAAGAAAATAAAACATTGGATATCTCCTCTATTTTATTCTTACCTATTGAATTAGCTTTCACTTAACTCCTTGGTGCTGAATTCATCACGGAAAAAGCTTTTGTTTCTTAAATTAAATAAAGGGTAGTGTTTGATGAGAAAGTCTAAATGCTTGCTTTGGAATCTGTCACAGTTCTATCCTCCGGAGGATTGGCTAGTTGGTTCGTCTTTGCCTTCGAAGTTCAATCCAAACTTGATAGGAATGGTTCTGGATCAGCTTTCTGTAGATAATGTCCGGTAAGTTTCTGGTCAGTTAGATTCTCATTGAGCATTAAAAGCCTTAAAATGGAAGCTGTGTATAGAATAATGAAACGCAATTTGACAGTTAACAGGATATTATCTTTTCTGTATACATTTATTCCTTATAATTGTTTACAATAAGACAGATCACTGGTCTCGTTGTTTCTTCTCTCCCCTTATTTCCTTCATGGTCATTGTGAAATGTAGTTTATGTTTGTTGTTGCTAATAGTATAATTAAAATTATACGAAACTATTTGTACAGGTATCAAATGTAAGACATGAATAAGTATGAACTATTTATGTTTTGTTTGAGGTGGGAAGGTTAATGAAATTTTACTTAACAAACTTTATTTTGAATTTTGTAATGTAATTATTTTTAATCTTTTTTCTTGATTTTCTTTCCCCTTTTTTTCCTACTAAAACCCTGTCCTTGAAATGATGAAAAAATTTATAAAGCTCGAGTATGCAAACTCCCACAGAGAGTGATTAGAACAAAACACCTCACTTCAGTAAAAATTCAAAGCCAAAAATAACCAAAATAGAAGAAAACAAGCCCTCCAAGAACAAAAAGGACCCAACTAGACAAAGCCTTAGTTTGGGATTGAAGTCCCTTTTTGCAGTTTGGGGTTTGGTTCCCCTTATCGTGTTATTTGATTGAAGCCATTTTCCATCAGAGCATTATTTTTCTTGCCCTCTAATGTTCAACTCCTAAACCACCGTGCTTGGAGAACCTTAACCCAATACCGTTAATATATATATATATATATATATATATATTTTAACTTGAATTTTTAAGGAAGAAATAACTCAATGTTTTTCCTAATTCAAATTGAAATATTTTAGAGTTTGTTAATTATCAATATATAGGTACTTTTTCTACTGAACCGTTTGCCTAAACAGCATGGTGTAGTGGCTGCATTATAATTTTGTAAATTTTACTCTCTTTGGAAATTAAGATTTGTCTTAATAATTTTTTAGCCGATTGAAAGTTTTTTTTTTTTTTTTAAATAATAATTCATATTTTATCTATCAATAAAATGTTTGTTTCCTATTATAGAAAACGTATTGCAACATGATTAATTTATGTTCTGTAGCATCTTTTGGGAGTCAAAGAACTTTGAAGGCAAGACCGATAAGGTTGAGCAGTGGTATGGAACTGCTTACTCCATCGAGAAAATTTCTGGCCCTTTGATTCAGGTTTGTCGTTTAATAGTAATTTTGTTTTTACTAGAAATGTGTATTTCGATTAAAAATTTAGGAGAAACTTTTAAAAAATTGGGAAAAATTCAATTTTACCCTTGCATTTGAAAATATGAGTTCAAATTTATCCTAAAACTTTGATTTTATCAAATGAGACCTTAGATTGATGACCGTTGTAATTTTTACTTTTATTTAAGCCTTTCGTCACATCCAAACCCAATCACTAATTCCAACACAAAATTTCTATCAAAATTACTAACTTTTGAAAAAGTCAAGCTCTAAAAAATTAATCTAGAATTGGAACTAAAAAATTATGCTATTTCAATTGAGTAGAGGATTATGCTAATCTCTATCAAATTTACTAATTTTGAAAATGTAAGTCTATTGATGGGAAATGAAACTACGGTTTTATTTACTGTCTGCAGGATTGGATACAATCTGCTCCTGATGTAAAATTGCATCTACCAGCTTCAAACGTTTTCATCCCTACTGATTTATCACTCAAGGGTGCATGTGAAAAGGTTTAATATGAACTTCTATTCATACTAAGAAGATGTTATTCCTTTTGTTGGTTTGTATTTTTCCTAATATAGTTGGCTGTTGAAATAGGTGGAGTTTCCAATTCTGCTAAGAAAGTCATCATATTCGACAATATGGTACAAACCTGATACAATGTTCTTCACGCCCAAGGCATATGTTAAGATTGATTTTAAATGTCCCCATGCTGACATCTCACCAGAAGCAGAAGTCCTAACGGAAATTTTCACGAGGTTATTGATGGATTATCTGAATGAATATGGTAAGTTATTCCAAGTTGATGAAGTATTTGGCTAGTTTTTTCTTTCTTTCTTTTCCTTTATTGTTCTGTTGGTTTGAATTTCTGTCAATTTTTCTGAGAATAGCATAATCTTCTGCCGATCCTTCTTGAAACTTCACTTGTATTTTACATAAATATTTTATTTTATATGCCTCTCTAATTTAATTTTTTAGGGAAGAATACTTTTTTAGTCCATGCATTTTGAGTATCGAGTATGGTTTCTATTTAGTTCCTAAGTTTTAAAATGTTACACTTTTGCCCATGAGTTTTGAGTTTAGTTTCCATTTGGTCCGTAAGATTTCACTGATGCATACCTTTTGTCATATTTGTGTTAATGTGATGTTAAGTAATTTAAAATAATTAAATACAGACCAAAACTATATTCAAAACTTACAAGCATCCTAAGGGATGGAAGCTAAATCTTTCTTGTCTTGCAGCTTATAGTGCTCAGGTTGCTGGACTGTATTATGGCATAAGCCCTGCAGACTCTGGTTTCCAGGTACCATTTTGTTCCAGAATAGGAATTTTCTTTCAATGATGTAAAAGTGGCGATACATAAAACCTGTTTAGCCAAAAGATCAAGGAGTTGGTGTTAAGTTAATTTTCCTTCAAATCTCAAGTTAAAAAAGGATTTAGGAATTTTCTATTACGTTGGAATATGGTGGTTATAAGGTCCTTTGTTGGTGTTTCTTTGCATGCCTCATCTTGTTGGACCGGCTAACCCACCTTTTTAAGATTCCTCAGATTCCTATGTCGTGTTTAAGGAGCAAGTTGTCTTCTTCATCATTCTTATCGATGGATTGAAGTTTCTTCATACACAGTTTGTTTTGAAAGAAATCAAAGAATTTTCAACAAGTATCCTCTTTGGTCAGATCTATTAGTTTAGGATTTTTTGAGAGAAACATGTGAAGGATGGTAGAATAGGTAATTTTAAAGGTTGATTTCCTAGCACTTTATTGTTTTGATTCTAAAAGCAATGGAATTGGTTTTTGCTGAGATGTTTGAAGCAAAGGGTATAGTATCTGAGTTGTCAATGCTTATAAGTTTTGAAGTTTATGCTGGATTTTGTTTCTAGTGCATGAAGATGAATTAGTCTTAGATGTTTTATTTTCAAAAGAGAAGTTGATAGCAAACTTATAGCTCTTGTCGAAGAATGTGGTTTCGAGGCAATCTATTCTTTCTTGTTTCCTCTTTCTGGATTTGTTTCTCCTGTAGTTCTCTATAATGGTTTTGCTTCCAGTTTCCAGAGAACATAAAAGTTCTTGGGCGATTTTTCTTGCTAGTAGCTGTCCTGAAATAGTGTAACTTATAGCTACGATGTTTTTTTTTTTTCTTGAAGAAGTGGATTAGAACTTACTTGTCTCGTAGATCTTTCAAGTTAGGCCTTTATCATTTGTAAAGCTTAGATACCTTTTTAAGATTTTTTCTTTTCGAGCTTGTTTGGTTTGGACTAGTGTATTTTGTTTGAATTTATTCTTCTGACATGTTGATAAGCTGTTGTAATTTTCCATTGCATTCGATGAAAATTCTGCTTAAAAGAAAAGGTTAATATTCAGCATTATGTTCGAGAGGCTTTAGTTGCTGCTATGTTTGTGTACATTGTTGCCTCACACCATTGACTTCAAACCACAAGGAGCTAACGCATGGTAGCTTTATGAGATCCGTAAATACTTTTTTAAGTTGAATTTACTTTGCTGGATTACTTGAATGCTATTCAGTTACTCTCTTCATTTTTGTTCCATCTGGTTCAGGTAGTTGATAATGCTTTGTAGAAAGTGTATAATTTGTTCTCATATCCTAAGAAAGTTGATAAAAATGGGACAAGTATTTTGTTTTGGACCATAAATAGAGTACATATTTCATAATCTTACCGGTAATTTGCTAAAAAGTACTCATTTTTGTGATATGCTCACAGTTATTCTGGTGAATGATCTCTGAAAACGGATACTAGATCATTAGTACCGTAATTTGTCACTTTCCCATAAAATTCTTAATTTCTCTGTTGTTTTTCATTATTGTTCTTTAGGTGACTCTGATTGGTTACAACCACAAATTGAGGATTCTATTGGAAACTATAGTTGAAAAAATTGCAAACTTCAGTGTGAAACCTGATAGGTTTCTCGTCATCAAGGTATTGTTATCATTCATTTGCATAAGTTTCTAACATAGAAATCTTATTATCTTAATTAATTAGAGTTGGAACTTGAATAATAACTATCAAGTATTGAAATATTTAAATTTCAGTAGATAGGAAAAGACTAGTAATAATACGGTATGGCAAGGGATTGCATCATTCAGTCAAGGATTTTAGAACGAAACCAATAATATTATACTTTTTCATTCATTCAATCAATAAAATATTCAATTTTTCTTTATTTAAAAGAATACGTACAAAAGTGAATATTTAAATACCTCAACTAAGAAGGAAAAAAAATTGAGTCACCAAATTTAGTGGGTGCATTTGTTCAAGAAGTGTCTTATCTAACATTCGATCTTTCTATCAGGAAACTGTGTTAAAGGAGTACCAAAATTTCAAGTTTCAGAAGCCATATCAGCAGGCTTTGTACTATTGTTCGTTAATACTTCAAGATCACACATGGCCATTAATGGAGGAACTTGCAATCCTTCCACATTTGGGGGCAGAAGATCTAGTTAAATTTGCTCCCACTTTGCTCTCAAGTGCCTACTTGGAATGTTATATTGCAGGTCTGTATCATTCTCTTTCGTCTTGACTATCTTACGGATCACAAGCTTTTGGCTTTAATGGTTAATTACTCAGGAAACATTGAAAGGAATGAATCCGAAACAATGATCGAACACATTGAAGATATGTTCTTCAAGGGGACAAATCCAATATCACGACCATTATATCCATCACAGCATCCAACCAATAGAGTTGTGAAGCTTGAGAGGGGCATTGGTTATTTCTATTCAGCAGAGGGTCTCAATCCAAACGATGAAAATTCCGCTCTTGTCCACTATATTCAGGTTATAAACTAATTCACTTCTTTGCCTTTAATATGAATATTTTTCTTTTGTATTTTCTGTGTTTTACTTTCCTCTTTTGTGTGTATGTAATGAAACTAATTCACTACAGTGAAAAATGATAAATTAAAAAGTGTAGGTATAATGTTGATATTACAGTTTATGCATAAGAAAAGAAAGGAACTCACTCCGTTACTCGAAGGAATTTGTGATGTCCCATATTGGTTGGGGAGGAGAACAAATCGCCCTTTATGAGGGTGTGGAAACCTTCCCCTAGTAGACGCGTTTTAAAACCTTGAGAGGAAGCCCGAAAGGGAAAGCCCAAGGAGGACAATATCTGCTAGCGGTGGATCTAGGTCGTTACAAATGGTATTAGAGCCAGTCACCGGACGATGTGCCAGCCTTCTCGCTGTTCCCTGAAGGGGGGTAGACACGAGGCGGTGTGCCAGTAAGGATGCTGGGCCCCAAAGGGAGGTGGATTTGGGGGCAGTCCCACATCGATTGGATAAAGGAACGAGTGCCAGCGAGGACGCTAGGCCCCGAAGGAGGGTGGATTGTGATGTCCCACGTTGGTTGGGGAGGAGAACAAATCACCCTTTATAAGGGTGTGGAAACCTTCCCCTATCAGACGCGTTTTAAAGCTTTGAGGGGAAGCCCAAAAGGAAAAGCCCAAAGAGGACAATATCTGCTAGCGGTGGATCTGGGTCGTTACAGAATTCCTCCTTAACTCTTCTTTGGTTTAAGGTTTGATGCACTTTGCTAAGTACCTTTTCTTCAATATTTAGGTGCATCGAGATGATTTCCTACAGAATGTGAAGCTTCAGCTTTTTGCTCTTGTGGCAAAACAAGCAGCTTTTCATCAGCTTAGAACTGTTGAACAACTTGGCTATATTACTGTTCTAATGCAAAGGTTAGAACATTTTGTAACTCACCTTTATTGAAATATTTACCGTGTAATTAATTTATCTTCAAATACAGGAACGACTCTGGTATACGTGGAGTTCAATTTATTATTCAATCCACTGTCAAGGTAAGGATCATTATCTCATCTGCTACCGTATCCTTGACATGTCATGGATTTTATCCTTATTTTGATGTGGCAGGGTCCTCGAAATATTGATTTGAGAGTAGAATCGTTCCTAGAGATGTTTGAGAAGAAGCTCGTCGATATGACCATTGAAGAATTCAAGGTGAGAGCAGTAATTGCACTATATTATTTCTATAACTCATATGATATGCAGTGTTAAGTTACCATTTGTATGTGACGTCTTAACAATGTGGTGGCTTCATGGTTTCTGACAAAATAAACTTTTTTAGGAAAAATTATCTTTTTTGCCCTTTAAATTTGGTGGACAATAGGACAGTTTATTCCTAAGTGTCAAAATCTTAGGTTCTTAAATTTCAAAATCTAGCACTTTTGTCCGTTAGGCTTAGAAATACTAAAATTAGGTCCTCGAAGTTCGATTTCCATTAGTTAATTGAAAGCAATGATGTGTTATATTTTTATTATTAAAATTATTGACGTGACAAATTTAATAATAAAAAGTGTTAAATTACAAGTTTAGCTCATGAAATTTGCCTGGTTCGGATACAAACCTCATCGTGACTGCAAGCCACAAATGCTCTATTGTCCTGTTCGGATACACATGCTTTATACTTAATCGTCCTGTTATGGATGGTGGTTGTGCAGAGCAATGTAAATGCTCTAGTAGATATGAAGCTTGAGAAATTCAAGAACTTGAGGGAGGAATCTGGATTTTACTGGCGAGAGATTGCTGATGGGACAATGAAATTCGACAGGAGAGAATCAGAGGTATGATATCAAATTATTATGCTCCAGTTTTTCATACAATGTTTCGGGGCATGATATGCACTGTTGGCTATTACCGTTGGTTTTAACCAGGTTGCGGCATTAAAGACACTCACACATCAGGATTTGATCAACTTCTTCAATGAACATATAAAAGTTGGTGCACCTCGTAAGAGGTCTTTAAGCGTTCGGGTTTACGGAAACCTTCACTCCTCAGAGTATTCTGCAGATCTAGATCAACCACTTCAACCCGACACAGTAAAAATAGATGATATTTTCAGCTTCAGAAGATCACAGCCACTTTATGGTTCCTTCAAGGGAGCGTTTGGTAATGTGAAGTTGTAGGTAATGGCTTCGAACTCCCTTGTCTCAAGCATTTTCATATTGGATTTTCTCAACTCAGCTCTTAACAAGTGAGGCATTGAATGTGAGTTATCTAGTGAATGAGCTGAATCAGCAGCAGATCTGTGCAGCAAATTTGATTTGTTGCGAAGTGAAGATGCTCTGAATCCTCCAAGTGGCCTTCTGTGTTAGCCAATAAAAGAACCGCTCAAACAAGCCCTTGCATTTGGTACTTATTATTATAGTTCTCATTCGCCCTTGAATAATGTGACAATTTTGTGCTCCATTTACTAACCCTCCTGTGGGTGACATCATAATTCTTTCTCTGTTTCAACTATATTCCATGGTCTTTGTGCATTTGCATGTTCATGTTTGCTTCTTTTATTCAAGTCAAGCGTGGGTGGGATTATTGCTTTGCTTGGTGAACAACCTTAATTTTATATAAATGTATTATTTTCTTTTCT

mRNA sequence

CAAAATCCATTTGCTCTCAGGTTACGATTTTTATCGTTTCAAGCAAAATTTGTCCAAAGACTTCGTATAATCACCGATCAAGGTGCTGAGTTTTCGATTCAATACTCTCCTTCTCTGTTGGGGATAGAAGCAATGGCGGTTGGTAAGGAACAGGTGACTCATGACATAGTGAAGCCTCGTACTGACAAAAGAGAGTACAGAAGAATCGTCCTCAGGAACTCCCTTGAGGTTCTGCTAGTCAGTGATCCTGATACCGACAAGAGTGCTGCTTCCATGACTGTTAACGTTGGTTCCTTCAGCGACCCCGAAGGCCTAGAGGGTTTAGCCCATTTTCTTGAGCATATGCTGTTTTATGCAAGTGAAAAGTATCCATTGGAGGATAGTTACTCGAAATATATCACAGAGCATGGAGGTAGCATGAATGCCTTTACGGCATCTGAGCAGACAAATTATTATTTTGAGGTCAATGTTGATTGTTTTGAGGAGGCTTTGGACAGATTTGCTCAATTTTTCATCAAACCTTTAATGTCGCCTGATGCTACAATGAGAGAAATCAAAGCGGTTGATTCTGAGAATCAAAAGAATTTATTGTCTGATGGCTGGAGAATGCACCAGCTTCAGAGACATATAAGTTCTGAAAGTCATCCATTTCATAAATTCAGCACAGGGAATTGGGACACTTTAGAGGTTCAACCAAAAGCTAAAGGGCTGGATACAAGGCATGAACTTCTAAAATTTTATAAAAATAATTATTCATCCAATGTCATGCATCTGGTTGTATATGCAAAAGAAAACCTCGATAAAGTACAAAGCCTTGTGGAAAACATATTTCAAGATATTCCAAATAATAACTGCAATCGCGCCAATTTTCCTGGTCAGCCATGCATGTCAGAACATCTTCAGGTTCTTGTTAGAGCCATCCCAATTAAACAAGGTCATAAATTGAGAATTGTTTGGCCAATAACTCCTGAGATTCATCATTACAAGGAAGGGCCATGCCGGTATCTGGGTCATCTTATTGGCCATGAAGGAGAAGGATCCTTGTATTATGTTCTTAAAACATTAGGGTGGGCAACAGGTCTCTCAGCCGGGGAATCTGATTCCACGATGAATTTTTCCTTTTTCAAAGTAGGAATAGATCTCACTGATGTTGGTCAAGAACACATGCAGGATGTTGTTGGGCTTCTCTTTAAATACATCAGCCTTTTGAAGGAATCTGGTATTTGCCAATGGATATTTGATGAGCTTTCAGCTATTTGTGAGACGAAGTTCCATTATCAAGATAAAATTCGTCCAATTGACTATGTAGTCAATCTTTCATTAAATATGCAGTTCTATCCTCCGGAGGATTGGCTAGTTGGTTCGTCTTTGCCTTCGAAGTTCAATCCAAACTTGATAGGAATGGTTCTGGATCAGCTTTCTGTAGATAATGTCCGCATCTTTTGGGAGTCAAAGAACTTTGAAGGCAAGACCGATAAGGTTGAGCAGTGGTATGGAACTGCTTACTCCATCGAGAAAATTTCTGGCCCTTTGATTCAGGATTGGATACAATCTGCTCCTGATGTAAAATTGCATCTACCAGCTTCAAACGTTTTCATCCCTACTGATTTATCACTCAAGGGTGCATGTGAAAAGGTGGAGTTTCCAATTCTGCTAAGAAAGTCATCATATTCGACAATATGGTACAAACCTGATACAATGTTCTTCACGCCCAAGGCATATGTTAAGATTGATTTTAAATGTCCCCATGCTGACATCTCACCAGAAGCAGAAGTCCTAACGGAAATTTTCACGAGGTTATTGATGGATTATCTGAATGAATATGCTTATAGTGCTCAGGTTGCTGGACTGTATTATGGCATAAGCCCTGCAGACTCTGGTTTCCAGGTACCATTTTGTTCCAGAATAGGAATTTTCTTTCAATGATGTAAAAGTGGCGATACATAAAACCTGTTTAGCCAAAAGATCAAGGAGTTGGTGTTAAGTTAATTTTCCTTCAAATCTCAAGTTAAAAAAGGATTTAGGAATTTTCTATTACGTTGGAATATGGTGGTTATAAGGTCCTTTGTTGGTGTTTCTTTGCATGCCTCATCTTGTTGGACCGGCTAACCCACCTTTTTAAGATTCCTCAGATTCCTATGTCGTGTTTAAGGAGCAAGTTGTCTTCTTCATCATTCTTATCGATGGATTGAAGTTTCTTCATACACAGTTTGTTTTGAAAGAAATCAAAGAATTTTCAACAAGTATCCTCTTTGGTCAGATCTATTAGTTTAGGATTTTTTGAGAGAAACATGTGAAGGATGGTAGAATAGGTAATTTTAAAGGTTGATTTCCTAGCACTTTATTGTTTTGATTCTAAAAGCAATGGAATTGGTTTTTGCTGAGATGTTTGAAGCAAAGGGTATAGTATCTGAGTTGTCAATGCTTATAAGTTTTGAAGTTTATGCTGGATTTTGTTTCTAGTGCATGAAGATGAATTAGTCTTAGATGTTTTATTTTCAAAAGAGAAGTTGATAGCAAACTTATAGCTCTTGTCGAAGAATGTGGTTTCGAGGCAATCTATTCTTTCTTGTTTCCTCTTTCTGGATTTGTTTCTCCTGTAGTTCTCTATAATGGTTTTGCTTCCAGTTTCCAGAGAACATAAAAGTTCTTGGGCGATTTTTCTTGCTAGTAGCTGTCCTGAAATAGTGTAACTTATAGCTACGATGTTTTTTTTTTTTCTTGAAGAAGTGGATTAGAACTTACTTGTCTCGTAGATCTTTCAAGTTAGGCCTTTATCATTTGTAAAGCTTAGATACCTTTTTAAGATTTTTTCTTTTCGAGCTTGTTTGGTTTGGACTAGTGTATTTTGTTTGAATTTATTCTTCTGACATGTTGATAAGCTGTTGTAATTTTCCATTGCATTCGATGAAAATTCTGCTTAAAAGAAAAGGTTAATATTCAGCATTATGTTCGAGAGGCTTTAGTTGCTGCTATGTTTGTGTACATTGTTGCCTCACACCATTGACTTCAAACCACAAGGAGCTAACGCATGGTAGCTTTATGAGATCCGTAAATACTTTTTTAAGTTGAATTTACTTTGCTGGATTACTTGAATGCTATTCAGTTACTCTCTTCATTTTTGTTCCATCTGGTTCAGGTAGTTGATAATGCTTTGTAGAAAGTGTATAATTTGTTCTCATATCCTAAGAAAGTTGATAAAAATGGGACAAGTATTTTGTTTTGGACCATAAATAGAGTACATATTTCATAATCTTACCGGTAATTTGCTAAAAAGTACTCATTTTTGTGATATGCTCACAGTTATTCTGGTGAATGATCTCTGAAAACGGATACTAGATCATTAGTACCGTAATTTGTCACTTTCCCATAAAATTCTTAATTTCTCTGTTGTTTTTCATTATTGTTCTTTAGGTGACTCTGATTGGTTACAACCACAAATTGAGGATTCTATTGGAAACTATAGTTGAAAAAATTGCAAACTTCAGTGTGAAACCTGATAGGTTTCTCGTCATCAAGGAAACTGTGTTAAAGGAGTACCAAAATTTCAAGTTTCAGAAGCCATATCAGCAGGCTTTGTACTATTGTTCGTTAATACTTCAAGATCACACATGGCCATTAATGGAGGAACTTGCAATCCTTCCACATTTGGGGGCAGAAGATCTAGTTAAATTTGCTCCCACTTTGCTCTCAAGTGCCTACTTGGAATGTTATATTGCAGGAAACATTGAAAGGAATGAATCCGAAACAATGATCGAACACATTGAAGATATGTTCTTCAAGGGGACAAATCCAATATCACGACCATTATATCCATCACAGCATCCAACCAATAGAGTTGTGAAGCTTGAGAGGGGCATTGGTTATTTCTATTCAGCAGAGGGTCTCAATCCAAACGATGAAAATTCCGCTCTTGTCCACTATATTCAGGTGCATCGAGATGATTTCCTACAGAATGTGAAGCTTCAGCTTTTTGCTCTTGTGGCAAAACAAGCAGCTTTTCATCAGCTTAGAACTGTTGAACAACTTGGCTATATTACTGTTCTAATGCAAAGGAACGACTCTGGTATACGTGGAGTTCAATTTATTATTCAATCCACTGTCAAGGGTCCTCGAAATATTGATTTGAGAGTAGAATCGTTCCTAGAGATGTTTGAGAAGAAGCTCGTCGATATGACCATTGAAGAATTCAAGAGCAATGTAAATGCTCTAGTAGATATGAAGCTTGAGAAATTCAAGAACTTGAGGGAGGAATCTGGATTTTACTGGCGAGAGATTGCTGATGGGACAATGAAATTCGACAGGAGAGAATCAGAGGTTGCGGCATTAAAGACACTCACACATCAGGATTTGATCAACTTCTTCAATGAACATATAAAAGTTGGTGCACCTCGTAAGAGGTCTTTAAGCGTTCGGGTTTACGGAAACCTTCACTCCTCAGAGTATTCTGCAGATCTAGATCAACCACTTCAACCCGACACAGTAAAAATAGATGATATTTTCAGCTTCAGAAGATCACAGCCACTTTATGGTTCCTTCAAGGGAGCGTTTGGTAATGTGAAGTTGTAGGTAATGGCTTCGAACTCCCTTGTCTCAAGCATTTTCATATTGGATTTTCTCAACTCAGCTCTTAACAAGTGAGGCATTGAATGTGAGTTATCTAGTGAATGAGCTGAATCAGCAGCAGATCTGTGCAGCAAATTTGATTTGTTGCGAAGTGAAGATGCTCTGAATCCTCCAAGTGGCCTTCTGTGTTAGCCAATAAAAGAACCGCTCAAACAAGCCCTTGCATTTGGTACTTATTATTATAGTTCTCATTCGCCCTTGAATAATGTGACAATTTTGTGCTCCATTTACTAACCCTCCTGTGGGTGACATCATAATTCTTTCTCTGTTTCAACTATATTCCATGGTCTTTGTGCATTTGCATGTTCATGTTTGCTTCTTTTATTCAAGTCAAGCGTGGGTGGGATTATTGCTTTGCTTGGTGAACAACCTTAATTTTATATAAATGTATTATTTTCTTTTCT

Coding sequence (CDS)

CAAAATCCATTTGCTCTCAGGTTACGATTTTTATCGTTTCAAGCAAAATTTGTCCAAAGACTTCGTATAATCACCGATCAAGGTGCTGAGTTTTCGATTCAATACTCTCCTTCTCTGTTGGGGATAGAAGCAATGGCGGTTGGTAAGGAACAGGTGACTCATGACATAGTGAAGCCTCGTACTGACAAAAGAGAGTACAGAAGAATCGTCCTCAGGAACTCCCTTGAGGTTCTGCTAGTCAGTGATCCTGATACCGACAAGAGTGCTGCTTCCATGACTGTTAACGTTGGTTCCTTCAGCGACCCCGAAGGCCTAGAGGGTTTAGCCCATTTTCTTGAGCATATGCTGTTTTATGCAAGTGAAAAGTATCCATTGGAGGATAGTTACTCGAAATATATCACAGAGCATGGAGGTAGCATGAATGCCTTTACGGCATCTGAGCAGACAAATTATTATTTTGAGGTCAATGTTGATTGTTTTGAGGAGGCTTTGGACAGATTTGCTCAATTTTTCATCAAACCTTTAATGTCGCCTGATGCTACAATGAGAGAAATCAAAGCGGTTGATTCTGAGAATCAAAAGAATTTATTGTCTGATGGCTGGAGAATGCACCAGCTTCAGAGACATATAAGTTCTGAAAGTCATCCATTTCATAAATTCAGCACAGGGAATTGGGACACTTTAGAGGTTCAACCAAAAGCTAAAGGGCTGGATACAAGGCATGAACTTCTAAAATTTTATAAAAATAATTATTCATCCAATGTCATGCATCTGGTTGTATATGCAAAAGAAAACCTCGATAAAGTACAAAGCCTTGTGGAAAACATATTTCAAGATATTCCAAATAATAACTGCAATCGCGCCAATTTTCCTGGTCAGCCATGCATGTCAGAACATCTTCAGGTTCTTGTTAGAGCCATCCCAATTAAACAAGGTCATAAATTGAGAATTGTTTGGCCAATAACTCCTGAGATTCATCATTACAAGGAAGGGCCATGCCGGTATCTGGGTCATCTTATTGGCCATGAAGGAGAAGGATCCTTGTATTATGTTCTTAAAACATTAGGGTGGGCAACAGGTCTCTCAGCCGGGGAATCTGATTCCACGATGAATTTTTCCTTTTTCAAAGTAGGAATAGATCTCACTGATGTTGGTCAAGAACACATGCAGGATGTTGTTGGGCTTCTCTTTAAATACATCAGCCTTTTGAAGGAATCTGGTATTTGCCAATGGATATTTGATGAGCTTTCAGCTATTTGTGAGACGAAGTTCCATTATCAAGATAAAATTCGTCCAATTGACTATGTAGTCAATCTTTCATTAAATATGCAGTTCTATCCTCCGGAGGATTGGCTAGTTGGTTCGTCTTTGCCTTCGAAGTTCAATCCAAACTTGATAGGAATGGTTCTGGATCAGCTTTCTGTAGATAATGTCCGCATCTTTTGGGAGTCAAAGAACTTTGAAGGCAAGACCGATAAGGTTGAGCAGTGGTATGGAACTGCTTACTCCATCGAGAAAATTTCTGGCCCTTTGATTCAGGATTGGATACAATCTGCTCCTGATGTAAAATTGCATCTACCAGCTTCAAACGTTTTCATCCCTACTGATTTATCACTCAAGGGTGCATGTGAAAAGGTGGAGTTTCCAATTCTGCTAAGAAAGTCATCATATTCGACAATATGGTACAAACCTGATACAATGTTCTTCACGCCCAAGGCATATGTTAAGATTGATTTTAAATGTCCCCATGCTGACATCTCACCAGAAGCAGAAGTCCTAACGGAAATTTTCACGAGGTTATTGATGGATTATCTGAATGAATATGCTTATAGTGCTCAGGTTGCTGGACTGTATTATGGCATAAGCCCTGCAGACTCTGGTTTCCAGGTACCATTTTGTTCCAGAATAGGAATTTTCTTTCAATGA

Protein sequence

QNPFALRLRFLSFQAKFVQRLRIITDQGAEFSIQYSPSLLGIEAMAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGNWDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNNCNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLKESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPNLIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKLHLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHADISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQVPFCSRIGIFFQ
Homology
BLAST of Carg03529 vs. NCBI nr
Match: KAG7017597.1 (Insulin-degrading enzyme-like 1, peroxisomal, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1319.7 bits (3414), Expect = 0.0e+00
Identity = 641/641 (100.00%), Postives = 641/641 (100.00%), Query Frame = 0

Query: 1   QNPFALRLRFLSFQAKFVQRLRIITDQGAEFSIQYSPSLLGIEAMAVGKEQVTHDIVKPR 60
           QNPFALRLRFLSFQAKFVQRLRIITDQGAEFSIQYSPSLLGIEAMAVGKEQVTHDIVKPR
Sbjct: 1   QNPFALRLRFLSFQAKFVQRLRIITDQGAEFSIQYSPSLLGIEAMAVGKEQVTHDIVKPR 60

Query: 61  TDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEGLEGLAHFLEHMLFYAS 120
           TDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEGLEGLAHFLEHMLFYAS
Sbjct: 61  TDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEGLEGLAHFLEHMLFYAS 120

Query: 121 EKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEALDRFAQFFIKPLMSPDA 180
           EKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEALDRFAQFFIKPLMSPDA
Sbjct: 121 EKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEALDRFAQFFIKPLMSPDA 180

Query: 181 TMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGNWDTLEVQPKAKGLDTR 240
           TMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGNWDTLEVQPKAKGLDTR
Sbjct: 181 TMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGNWDTLEVQPKAKGLDTR 240

Query: 241 HELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNNCNRANFPGQPCMSEHL 300
           HELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNNCNRANFPGQPCMSEHL
Sbjct: 241 HELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNNCNRANFPGQPCMSEHL 300

Query: 301 QVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEGEGSLYYVLKTLGWATG 360
           QVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEGEGSLYYVLKTLGWATG
Sbjct: 301 QVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEGEGSLYYVLKTLGWATG 360

Query: 361 LSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLKESGICQWIFDELSAIC 420
           LSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLKESGICQWIFDELSAIC
Sbjct: 361 LSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLKESGICQWIFDELSAIC 420

Query: 421 ETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPNLIGMVLDQLSVDNVRI 480
           ETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPNLIGMVLDQLSVDNVRI
Sbjct: 421 ETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPNLIGMVLDQLSVDNVRI 480

Query: 481 FWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKLHLPASNVFIPTDLSLK 540
           FWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKLHLPASNVFIPTDLSLK
Sbjct: 481 FWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKLHLPASNVFIPTDLSLK 540

Query: 541 GACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHADISPEAEVLTEIFTRL 600
           GACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHADISPEAEVLTEIFTRL
Sbjct: 541 GACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHADISPEAEVLTEIFTRL 600

Query: 601 LMDYLNEYAYSAQVAGLYYGISPADSGFQVPFCSRIGIFFQ 642
           LMDYLNEYAYSAQVAGLYYGISPADSGFQVPFCSRIGIFFQ
Sbjct: 601 LMDYLNEYAYSAQVAGLYYGISPADSGFQVPFCSRIGIFFQ 641

BLAST of Carg03529 vs. NCBI nr
Match: KAG6580840.1 (Insulin-degrading enzyme-like 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1295.4 bits (3351), Expect = 0.0e+00
Identity = 630/630 (100.00%), Postives = 630/630 (100.00%), Query Frame = 0

Query: 1    QNPFALRLRFLSFQAKFVQRLRIITDQGAEFSIQYSPSLLGIEAMAVGKEQVTHDIVKPR 60
            QNPFALRLRFLSFQAKFVQRLRIITDQGAEFSIQYSPSLLGIEAMAVGKEQVTHDIVKPR
Sbjct: 928  QNPFALRLRFLSFQAKFVQRLRIITDQGAEFSIQYSPSLLGIEAMAVGKEQVTHDIVKPR 987

Query: 61   TDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEGLEGLAHFLEHMLFYAS 120
            TDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEGLEGLAHFLEHMLFYAS
Sbjct: 988  TDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEGLEGLAHFLEHMLFYAS 1047

Query: 121  EKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEALDRFAQFFIKPLMSPDA 180
            EKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEALDRFAQFFIKPLMSPDA
Sbjct: 1048 EKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEALDRFAQFFIKPLMSPDA 1107

Query: 181  TMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGNWDTLEVQPKAKGLDTR 240
            TMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGNWDTLEVQPKAKGLDTR
Sbjct: 1108 TMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGNWDTLEVQPKAKGLDTR 1167

Query: 241  HELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNNCNRANFPGQPCMSEHL 300
            HELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNNCNRANFPGQPCMSEHL
Sbjct: 1168 HELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNNCNRANFPGQPCMSEHL 1227

Query: 301  QVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEGEGSLYYVLKTLGWATG 360
            QVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEGEGSLYYVLKTLGWATG
Sbjct: 1228 QVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEGEGSLYYVLKTLGWATG 1287

Query: 361  LSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLKESGICQWIFDELSAIC 420
            LSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLKESGICQWIFDELSAIC
Sbjct: 1288 LSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLKESGICQWIFDELSAIC 1347

Query: 421  ETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPNLIGMVLDQLSVDNVRI 480
            ETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPNLIGMVLDQLSVDNVRI
Sbjct: 1348 ETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPNLIGMVLDQLSVDNVRI 1407

Query: 481  FWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKLHLPASNVFIPTDLSLK 540
            FWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKLHLPASNVFIPTDLSLK
Sbjct: 1408 FWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKLHLPASNVFIPTDLSLK 1467

Query: 541  GACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHADISPEAEVLTEIFTRL 600
            GACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHADISPEAEVLTEIFTRL
Sbjct: 1468 GACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHADISPEAEVLTEIFTRL 1527

Query: 601  LMDYLNEYAYSAQVAGLYYGISPADSGFQV 631
            LMDYLNEYAYSAQVAGLYYGISPADSGFQV
Sbjct: 1528 LMDYLNEYAYSAQVAGLYYGISPADSGFQV 1557

BLAST of Carg03529 vs. NCBI nr
Match: XP_023526970.1 (insulin-degrading enzyme-like 1, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1212.2 bits (3135), Expect = 0.0e+00
Identity = 586/586 (100.00%), Postives = 586/586 (100.00%), Query Frame = 0

Query: 45  MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG 104
           MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG
Sbjct: 1   MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG 60

Query: 105 LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL 164
           LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL
Sbjct: 61  LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL 120

Query: 165 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN 224
           DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN
Sbjct: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN 180

Query: 225 WDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNN 284
           WDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNN
Sbjct: 181 WDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNN 240

Query: 285 CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 344
           CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG
Sbjct: 241 CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 300

Query: 345 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK 404
           EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK
Sbjct: 301 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK 360

Query: 405 ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN 464
           ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN
Sbjct: 361 ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN 420

Query: 465 LIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKL 524
           LIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKL
Sbjct: 421 LIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKL 480

Query: 525 HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA 584
           HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA
Sbjct: 481 HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA 540

Query: 585 DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV 631
           DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV
Sbjct: 541 DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV 586

BLAST of Carg03529 vs. NCBI nr
Match: XP_022934728.1 (insulin-degrading enzyme-like 1, peroxisomal [Cucurbita moschata])

HSP 1 Score: 1211.1 bits (3132), Expect = 0.0e+00
Identity = 585/586 (99.83%), Postives = 586/586 (100.00%), Query Frame = 0

Query: 45  MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG 104
           MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG
Sbjct: 1   MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG 60

Query: 105 LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL 164
           LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL
Sbjct: 61  LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL 120

Query: 165 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN 224
           DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN
Sbjct: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN 180

Query: 225 WDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNN 284
           WDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNN
Sbjct: 181 WDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNN 240

Query: 285 CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 344
           CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG
Sbjct: 241 CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 300

Query: 345 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK 404
           EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK
Sbjct: 301 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK 360

Query: 405 ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN 464
           ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN
Sbjct: 361 ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN 420

Query: 465 LIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKL 524
           LIGMVLDQLSVDNVRIFWESKNFEGKTD+VEQWYGTAYSIEKISGPLIQDWIQSAPDVKL
Sbjct: 421 LIGMVLDQLSVDNVRIFWESKNFEGKTDRVEQWYGTAYSIEKISGPLIQDWIQSAPDVKL 480

Query: 525 HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA 584
           HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA
Sbjct: 481 HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA 540

Query: 585 DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV 631
           DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV
Sbjct: 541 DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV 586

BLAST of Carg03529 vs. NCBI nr
Match: XP_022982829.1 (insulin-degrading enzyme-like 1, peroxisomal [Cucurbita maxima])

HSP 1 Score: 1206.8 bits (3121), Expect = 0.0e+00
Identity = 583/586 (99.49%), Postives = 585/586 (99.83%), Query Frame = 0

Query: 45  MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG 104
           MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG
Sbjct: 1   MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG 60

Query: 105 LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL 164
           LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL
Sbjct: 61  LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL 120

Query: 165 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN 224
           DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN
Sbjct: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN 180

Query: 225 WDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNN 284
           WDTLEVQPKAKGLDTRHELLKFY+NNYSSNVMHLVVYAKENLDKVQ LVENIFQDIPNNN
Sbjct: 181 WDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPNNN 240

Query: 285 CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 344
           CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPI+PEIHHYKEGPCRYLGHLIGHEG
Sbjct: 241 CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPISPEIHHYKEGPCRYLGHLIGHEG 300

Query: 345 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK 404
           EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK
Sbjct: 301 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK 360

Query: 405 ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN 464
           ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN
Sbjct: 361 ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN 420

Query: 465 LIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKL 524
           LIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKL
Sbjct: 421 LIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKL 480

Query: 525 HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA 584
           HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA
Sbjct: 481 HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA 540

Query: 585 DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV 631
           DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV
Sbjct: 541 DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV 586

BLAST of Carg03529 vs. ExPASy Swiss-Prot
Match: O22941 (Insulin-degrading enzyme-like 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=PXM16 PE=2 SV=1)

HSP 1 Score: 915.2 bits (2364), Expect = 4.1e-265
Identity = 426/589 (72.33%), Postives = 505/589 (85.74%), Query Frame = 0

Query: 45  MAVGKEQVT---HDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSD 104
           MAV K   T    +I+KPRTD REYR IVL+N L+VLL+SDPDTDK AASM+V+VGSFSD
Sbjct: 1   MAVEKSNTTVGGVEILKPRTDNREYRMIVLKNLLQVLLISDPDTDKCAASMSVSVGSFSD 60

Query: 105 PEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFE 164
           P+GLEGLAHFLEHMLFYASEKYP EDSYSKYITEHGGS NA+TASE+TNY+F+VN DCF+
Sbjct: 61  PQGLEGLAHFLEHMLFYASEKYPEEDSYSKYITEHGGSTNAYTASEETNYHFDVNADCFD 120

Query: 165 EALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFS 224
           EALDRFAQFFIKPLMS DATMREIKAVDSENQKNLLSDGWR+ QLQ+H+S E HP+HKFS
Sbjct: 121 EALDRFAQFFIKPLMSADATMREIKAVDSENQKNLLSDGWRIRQLQKHLSKEDHPYHKFS 180

Query: 225 TGNWDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIP 284
           TGN DTL V+P+AKG+DTR EL+KFY+ +YS+N+MHLVVY KE+LDK+Q LVE +FQ+I 
Sbjct: 181 TGNMDTLHVRPQAKGVDTRSELIKFYEEHYSANIMHLVVYGKESLDKIQDLVERMFQEIQ 240

Query: 285 NNNCNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIG 344
           N N     FPGQPC ++HLQ+LV+AIPIKQGHKL + WP+TP IHHY E P +YLGHLIG
Sbjct: 241 NTNKVVPRFPGQPCTADHLQILVKAIPIKQGHKLGVSWPVTPSIHHYDEAPSQYLGHLIG 300

Query: 345 HEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYIS 404
           HEGEGSL++ LKTLGWATGLSAGE + T+++SFFKV IDLTD G EHMQ+++GLLF YI 
Sbjct: 301 HEGEGSLFHALKTLGWATGLSAGEGEWTLDYSFFKVSIDLTDAGHEHMQEILGLLFNYIQ 360

Query: 405 LLKESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKF 464
           LL+++G+CQWIFDELSAICETKFHYQDKI P+ Y+V+++ NMQ YP +DWLVGSSLP+KF
Sbjct: 361 LLQQTGVCQWIFDELSAICETKFHYQDKIPPMSYIVDIASNMQIYPTKDWLVGSSLPTKF 420

Query: 465 NPNLIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPD 524
           NP ++  V+D+LS  N RIFWES+ FEG+TDK E WY TAYS+EKI+   IQ+W+QSAPD
Sbjct: 421 NPAIVQKVVDELSPSNFRIFWESQKFEGQTDKAEPWYNTAYSLEKITSSTIQEWVQSAPD 480

Query: 525 VKLHLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKC 584
           V LHLPA NVFIPTDLSLK A +K   P+LLRK+ +S +WYKPDTMF  PKAYVK+DF C
Sbjct: 481 VHLHLPAPNVFIPTDLSLKDADDKETVPVLLRKTPFSRLWYKPDTMFSKPKAYVKMDFNC 540

Query: 585 PHADISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV 631
           P A  SP+A VLT+IFTRLLMDYLNEYAY AQVAGLYYG+S +D+GF++
Sbjct: 541 PLAVSSPDAAVLTDIFTRLLMDYLNEYAYYAQVAGLYYGVSLSDNGFEL 589

BLAST of Carg03529 vs. ExPASy Swiss-Prot
Match: F4J3D9 (Insulin-degrading enzyme-like 2 OS=Arabidopsis thaliana OX=3702 GN=At3g57470 PE=3 SV=2)

HSP 1 Score: 867.5 bits (2240), Expect = 9.7e-251
Identity = 413/591 (69.88%), Postives = 485/591 (82.06%), Query Frame = 0

Query: 45  MAVGKEQVT-----HDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSF 104
           MAVG E  T      +I+KPRTDKREYRRIVL+NSLEVLL+SDP+TDK AASM V+VGSF
Sbjct: 1   MAVGMENATASGECGEILKPRTDKREYRRIVLKNSLEVLLISDPETDKCAASMNVSVGSF 60

Query: 105 SDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDC 164
           +DPEGLEGLAHFLEHMLFYASEKYP EDSYSKYITEHGGS NA+T+SE TNY+F++N D 
Sbjct: 61  TDPEGLEGLAHFLEHMLFYASEKYPEEDSYSKYITEHGGSTNAYTSSEDTNYHFDINTDS 120

Query: 165 FEEALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHK 224
           F EALDRFAQFFI+PLMS DATMREIKAVDSE+Q NLLSD WRM QLQ+H+S E HP+HK
Sbjct: 121 FYEALDRFAQFFIQPLMSTDATMREIKAVDSEHQNNLLSDSWRMAQLQKHLSREDHPYHK 180

Query: 225 FSTGNWDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQD 284
           FSTGN DTL V+P+  G+DTR EL+KFY  +YS+N+MHLVVY KENLDK Q LVE +FQ 
Sbjct: 181 FSTGNMDTLHVRPEENGVDTRSELIKFYDEHYSANIMHLVVYGKENLDKTQGLVEALFQG 240

Query: 285 IPNNNCNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHL 344
           I N N     FPGQPC  +HLQVLV+A+PI QGH+L + WP+TP I HY+E PCRYLG L
Sbjct: 241 IRNTNQGIPRFPGQPCTLDHLQVLVKAVPIMQGHELSVSWPVTPSISHYEEAPCRYLGDL 300

Query: 345 IGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKY 404
           IGHEGEGSL++ LK LGWATGL AGE+D +M +SFF V IDLTD G EHMQD++GLLF+Y
Sbjct: 301 IGHEGEGSLFHALKILGWATGLYAGEADWSMEYSFFNVSIDLTDAGHEHMQDILGLLFEY 360

Query: 405 ISLLKESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPS 464
           I +L++SG+ QWIFDELSAICE +FHYQ KI PI Y V++S NM+ YP + WLVGSSLPS
Sbjct: 361 IKVLQQSGVSQWIFDELSAICEAEFHYQAKIDPISYAVDISSNMKIYPTKHWLVGSSLPS 420

Query: 465 KFNPNLIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSA 524
           KFNP ++  VLD+LS +NVRIFWES  FEG+TDKVE WY TAYS+EKI+   IQ+W+QSA
Sbjct: 421 KFNPAIVQKVLDELSPNNVRIFWESNKFEGQTDKVEPWYNTAYSLEKITKFTIQEWMQSA 480

Query: 525 PDVKLHLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDF 584
           PDV L LP  NVFIPTD SLK   +K  FP+LLRK+SYS +WYKPDT FF PKAYVK+DF
Sbjct: 481 PDVNLLLPTPNVFIPTDFSLKDLKDKDIFPVLLRKTSYSRLWYKPDTKFFKPKAYVKMDF 540

Query: 585 KCPHADISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV 631
            CP A  SP+A VL++IF  LL+DYLNEYAY AQ AGL YG+S +D+GF++
Sbjct: 541 NCPLAVSSPDAAVLSDIFVWLLVDYLNEYAYYAQAAGLDYGLSLSDNGFEL 591

BLAST of Carg03529 vs. ExPASy Swiss-Prot
Match: Q24K02 (Insulin-degrading enzyme OS=Bos taurus OX=9913 GN=IDE PE=2 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 1.6e-144
Identity = 251/573 (43.80%), Postives = 359/573 (62.65%), Query Frame = 0

Query: 50  EQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEGLEGLA 109
           +++ H I+K   DKREYR + L N ++VLLVSDP TDKS+A++ V++GS SDP  + GL+
Sbjct: 48  KRIGHHIIKSHEDKREYRGLELANGIKVLLVSDPTTDKSSAALDVHIGSLSDPPNIAGLS 107

Query: 110 HFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEALDRFAQ 169
           HF EHMLF  ++KYP E+ YS++++EH GS NAFT+ E TNYYF+V+ +  E ALDRFAQ
Sbjct: 108 HFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQ 167

Query: 170 FFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGNWDTLE 229
           FF+ PL       RE+ AVDSE++KN+++D WR+ QL++   +  HPF KF TGN  TLE
Sbjct: 168 FFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLE 227

Query: 230 VQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNNCNRAN 289
            +P  +G+D R ELLKF+   YSSN+M + V  +E+LD + +LV  +F ++ N N     
Sbjct: 228 TRPNQEGIDVRQELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPE 287

Query: 290 FPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEGEGSLY 349
           FP  P   EHL+ L + +PIK    L + +PI     +YK  P  YLGHLIGHEG GSL 
Sbjct: 288 FPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLL 347

Query: 350 YVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLKESGIC 409
             LK+ GW   L  G+ +    F FF + +DLT+ G  H++D++  +F+YI  L+  G  
Sbjct: 348 SELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQ 407

Query: 410 QWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPNLIGMV 469
           +W+F E   +    F ++DK RP  Y   ++  + +YP E+ L    L  +F P+LI MV
Sbjct: 408 EWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMV 467

Query: 470 LDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKLHLPAS 529
           LD+L  +NVR+   SK+FEGKTD+ E+WYGT Y  E I   +I+ W  +  + K  LP  
Sbjct: 468 LDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPMK 527

Query: 530 NVFIPTDLS-LKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHADISP 589
           N FIPT+   L    E   +P L++ ++ S +W+K D  FF PKA +  +F  P A + P
Sbjct: 528 NEFIPTNFEILSLEKEATPYPSLIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDP 587

Query: 590 EAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGI 622
               +  ++  LL D LNEYAY+A++AGL Y +
Sbjct: 588 LHCNMAYLYLELLKDSLNEYAYAAELAGLSYDL 620

BLAST of Carg03529 vs. ExPASy Swiss-Prot
Match: P35559 (Insulin-degrading enzyme OS=Rattus norvegicus OX=10116 GN=Ide PE=1 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 6.1e-144
Identity = 252/574 (43.90%), Postives = 358/574 (62.37%), Query Frame = 0

Query: 50  EQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEGLEGLA 109
           +++   IVK   DKREYR + L N ++VLL+SDP TDKS+A++ V++GS SDP  + GL+
Sbjct: 48  QRIEDHIVKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIPGLS 107

Query: 110 HFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEALDRFAQ 169
           HF EHMLF  ++KYP E+ YS++++EH GS NAFT+ E TNYYF+V+ +  E ALDRFAQ
Sbjct: 108 HFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQ 167

Query: 170 FFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGNWDTLE 229
           FF+ PL       RE+ AVDSE++KN+++D WR+ QL++   +  HPF KF TGN  TLE
Sbjct: 168 FFLCPLFDASCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLE 227

Query: 230 VQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNNCNRAN 289
            +P  +G+D R ELLKF+   YSSN+M + V  +E+LD + +LV  +F ++ N N     
Sbjct: 228 TRPNQEGIDVREELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPE 287

Query: 290 FPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEGEGSLY 349
           FP  P   EHL+ L + +PIK    L + +PI     +YK  P  YLGHLIGHEG GSL 
Sbjct: 288 FPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQQYYKSNPGHYLGHLIGHEGPGSLL 347

Query: 350 YVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLKESGIC 409
             LK+ GW   L  G+ +    F FF + +DLT+ G  H++D++  +F+YI  L+  G  
Sbjct: 348 SELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQ 407

Query: 410 QWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPNLIGMV 469
           +W+F E   +    F ++DK RP  Y   ++  + +YP    L    L  +F P+LI MV
Sbjct: 408 EWVFQECKDLNAVAFRFKDKERPRGYTSKIAGKLHYYPLNGVLTAEYLLEEFRPDLIDMV 467

Query: 470 LDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKLHLPAS 529
           LD+L  +NVR+   SK+FEGKTD+ EQWYGT Y  E I   +IQ W  +  + K  LP  
Sbjct: 468 LDKLRPENVRVAIVSKSFEGKTDRTEQWYGTQYKQEAIPEDVIQKWQNADLNGKFKLPTK 527

Query: 530 NVFIPTDLSLKGACEK--VEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHADIS 589
           N FIPT+  +  A EK    +P L++ ++ S +W+K D  FF PKA +  +F  P A + 
Sbjct: 528 NEFIPTNFEIL-ALEKDATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVD 587

Query: 590 PEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGI 622
           P    +  ++  LL D LNEYAY+A++AGL Y +
Sbjct: 588 PLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDL 620

BLAST of Carg03529 vs. ExPASy Swiss-Prot
Match: Q9JHR7 (Insulin-degrading enzyme OS=Mus musculus OX=10090 GN=Ide PE=1 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 7.9e-144
Identity = 250/573 (43.63%), Postives = 357/573 (62.30%), Query Frame = 0

Query: 50  EQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEGLEGLA 109
           +++   IVK   DKREYR + L N ++VLL+SDP TDKS+A++ V++GS SDP  + GL+
Sbjct: 48  QRIEDQIVKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIPGLS 107

Query: 110 HFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEALDRFAQ 169
           HF EHMLF  ++KYP E+ YS++++EH GS NAFT+ E TNYYF+V+ +  E ALDRFAQ
Sbjct: 108 HFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQ 167

Query: 170 FFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGNWDTLE 229
           FF+ PL+      RE+ AVDSE++KN+++D WR+ QL++   +  HPF KF TGN  TLE
Sbjct: 168 FFLCPLLDASCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLE 227

Query: 230 VQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNNCNRAN 289
            +P  +G+D R ELLKF+   YSSN+M + V  +E+LD + +LV  +F ++ N N     
Sbjct: 228 TRPNQEGIDVREELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPE 287

Query: 290 FPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEGEGSLY 349
           FP  P   EHL+ L + +PIK    L + +PI     +YK  P  YLGHLIGHEG GSL 
Sbjct: 288 FPEHPFQEEHLRQLYKIVPIKDIRNLYVTFPIPDLQQYYKSNPGYYLGHLIGHEGPGSLL 347

Query: 350 YVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLKESGIC 409
             LK+ GW   L  G+ +    F FF + +DLT+ G  H++D++  +F+YI  L+  G  
Sbjct: 348 SELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQ 407

Query: 410 QWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPNLIGMV 469
           +W+F E   +    F ++DK RP  Y   ++  + +YP    L    L  +F P+LI MV
Sbjct: 408 EWVFQECKDLNAVAFRFKDKERPRGYTSKIAGKLHYYPLNGVLTAEYLLEEFRPDLIDMV 467

Query: 470 LDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKLHLPAS 529
           LD+L  +NVR+   SK+FEGKTD+ EQWYGT Y  E I   +IQ W  +  + K  LP  
Sbjct: 468 LDKLRPENVRVAIVSKSFEGKTDRTEQWYGTQYKQEAIPEDVIQKWQNADLNGKFKLPTK 527

Query: 530 NVFIPTDLS-LKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHADISP 589
           N FIPT+   L    +   +P L++ ++ S +W+K D  FF PKA +  +F  P A + P
Sbjct: 528 NEFIPTNFEILSLEKDATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDP 587

Query: 590 EAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGI 622
               +  ++  LL D LNEYAY+A++AGL Y +
Sbjct: 588 LHCNMAYLYLELLKDSLNEYAYAAELAGLSYDL 620

BLAST of Carg03529 vs. ExPASy TrEMBL
Match: A0A6J1F8G9 (insulin-degrading enzyme-like 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111441824 PE=3 SV=1)

HSP 1 Score: 1211.1 bits (3132), Expect = 0.0e+00
Identity = 585/586 (99.83%), Postives = 586/586 (100.00%), Query Frame = 0

Query: 45  MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG 104
           MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG
Sbjct: 1   MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG 60

Query: 105 LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL 164
           LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL
Sbjct: 61  LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL 120

Query: 165 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN 224
           DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN
Sbjct: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN 180

Query: 225 WDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNN 284
           WDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNN
Sbjct: 181 WDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNN 240

Query: 285 CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 344
           CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG
Sbjct: 241 CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 300

Query: 345 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK 404
           EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK
Sbjct: 301 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK 360

Query: 405 ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN 464
           ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN
Sbjct: 361 ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN 420

Query: 465 LIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKL 524
           LIGMVLDQLSVDNVRIFWESKNFEGKTD+VEQWYGTAYSIEKISGPLIQDWIQSAPDVKL
Sbjct: 421 LIGMVLDQLSVDNVRIFWESKNFEGKTDRVEQWYGTAYSIEKISGPLIQDWIQSAPDVKL 480

Query: 525 HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA 584
           HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA
Sbjct: 481 HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA 540

Query: 585 DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV 631
           DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV
Sbjct: 541 DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV 586

BLAST of Carg03529 vs. ExPASy TrEMBL
Match: A0A6J1J5M2 (insulin-degrading enzyme-like 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111481567 PE=3 SV=1)

HSP 1 Score: 1206.8 bits (3121), Expect = 0.0e+00
Identity = 583/586 (99.49%), Postives = 585/586 (99.83%), Query Frame = 0

Query: 45  MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG 104
           MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG
Sbjct: 1   MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG 60

Query: 105 LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL 164
           LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL
Sbjct: 61  LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL 120

Query: 165 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN 224
           DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN
Sbjct: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN 180

Query: 225 WDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNN 284
           WDTLEVQPKAKGLDTRHELLKFY+NNYSSNVMHLVVYAKENLDKVQ LVENIFQDIPNNN
Sbjct: 181 WDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPNNN 240

Query: 285 CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 344
           CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPI+PEIHHYKEGPCRYLGHLIGHEG
Sbjct: 241 CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPISPEIHHYKEGPCRYLGHLIGHEG 300

Query: 345 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK 404
           EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK
Sbjct: 301 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK 360

Query: 405 ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN 464
           ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN
Sbjct: 361 ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN 420

Query: 465 LIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKL 524
           LIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKL
Sbjct: 421 LIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKL 480

Query: 525 HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA 584
           HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA
Sbjct: 481 HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA 540

Query: 585 DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV 631
           DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV
Sbjct: 541 DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV 586

BLAST of Carg03529 vs. ExPASy TrEMBL
Match: A0A1S3B7B5 (insulin-degrading enzyme-like 1, peroxisomal isoform X1 OS=Cucumis melo OX=3656 GN=LOC103486819 PE=3 SV=1)

HSP 1 Score: 1125.5 bits (2910), Expect = 0.0e+00
Identity = 538/586 (91.81%), Postives = 564/586 (96.25%), Query Frame = 0

Query: 45  MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG 104
           MAVGKEQVTH+IVKPR DKREYRRIVL+NSLEVLLVSDPDTDKSAASMTV+VGSFSDPEG
Sbjct: 1   MAVGKEQVTHEIVKPRNDKREYRRIVLKNSLEVLLVSDPDTDKSAASMTVDVGSFSDPEG 60

Query: 105 LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL 164
           LEGLAHFLEHMLFYASEKYP EDSYSKYITEHGGSMNAFTASE+TNYYF+VN DCFEEAL
Sbjct: 61  LEGLAHFLEHMLFYASEKYPQEDSYSKYITEHGGSMNAFTASEETNYYFDVNADCFEEAL 120

Query: 165 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN 224
           DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQ HISSESHPFHKFSTGN
Sbjct: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQSHISSESHPFHKFSTGN 180

Query: 225 WDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNN 284
           W+TLEVQPKAKGLDTRHELLKFY+NNYSSNVMHLVVYAKE LDKVQ LVENIFQDIPN+N
Sbjct: 181 WETLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKEKLDKVQILVENIFQDIPNHN 240

Query: 285 CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 344
           CNRANFPGQPC SEHLQVLVRAIPIK+GHKLRI+WPITPEIHHYKEGPCRYLGHLIGHEG
Sbjct: 241 CNRANFPGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHYKEGPCRYLGHLIGHEG 300

Query: 345 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK 404
           EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKV IDLTDVGQEHMQ+V+GLLF+YISLLK
Sbjct: 301 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQEVIGLLFRYISLLK 360

Query: 405 ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN 464
           +SGICQWIFDELSAICETKFHY DKIRPI+YV++LSLNMQ YPPEDWLVGSSLPSKF+P 
Sbjct: 361 QSGICQWIFDELSAICETKFHYADKIRPINYVISLSLNMQLYPPEDWLVGSSLPSKFDPK 420

Query: 465 LIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKL 524
           LIG VLDQLSVDNVRIFWESK FEGKTDKVE+WYGTAYSIEKISG L+QDW+QSAPDV L
Sbjct: 421 LIGTVLDQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKISGHLVQDWMQSAPDVNL 480

Query: 525 HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA 584
           HLPASN+FIPTDLSLK ACEKVEFP+LLRKSSYS+IWYKPDTMF TPKAYVKIDF CPHA
Sbjct: 481 HLPASNIFIPTDLSLKRACEKVEFPVLLRKSSYSSIWYKPDTMFSTPKAYVKIDFICPHA 540

Query: 585 DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV 631
           DISPEAEVLT IFTRLLMDYLNEYAY A+VAGLYYGI+  DSGFQV
Sbjct: 541 DISPEAEVLTAIFTRLLMDYLNEYAYYAEVAGLYYGINLIDSGFQV 586

BLAST of Carg03529 vs. ExPASy TrEMBL
Match: A0A5A7TLF9 (Insulin-degrading enzyme-like 1, peroxisomal isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005990 PE=3 SV=1)

HSP 1 Score: 1125.5 bits (2910), Expect = 0.0e+00
Identity = 538/586 (91.81%), Postives = 564/586 (96.25%), Query Frame = 0

Query: 45  MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG 104
           MAVGKEQVTH+IVKPR DKREYRRIVL+NSLEVLLVSDPDTDKSAASMTV+VGSFSDPEG
Sbjct: 1   MAVGKEQVTHEIVKPRNDKREYRRIVLKNSLEVLLVSDPDTDKSAASMTVDVGSFSDPEG 60

Query: 105 LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL 164
           LEGLAHFLEHMLFYASEKYP EDSYSKYITEHGGSMNAFTASE+TNYYF+VN DCFEEAL
Sbjct: 61  LEGLAHFLEHMLFYASEKYPQEDSYSKYITEHGGSMNAFTASEETNYYFDVNADCFEEAL 120

Query: 165 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN 224
           DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQ HISSESHPFHKFSTGN
Sbjct: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQSHISSESHPFHKFSTGN 180

Query: 225 WDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNN 284
           W+TLEVQPKAKGLDTRHELLKFY+NNYSSNVMHLVVYAKE LDKVQ LVENIFQDIPN+N
Sbjct: 181 WETLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKEKLDKVQILVENIFQDIPNHN 240

Query: 285 CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 344
           CNRANFPGQPC SEHLQVLVRAIPIK+GHKLRI+WPITPEIHHYKEGPCRYLGHLIGHEG
Sbjct: 241 CNRANFPGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHYKEGPCRYLGHLIGHEG 300

Query: 345 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK 404
           EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKV IDLTDVGQEHMQ+V+GLLF+YISLLK
Sbjct: 301 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQEVIGLLFRYISLLK 360

Query: 405 ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN 464
           +SGICQWIFDELSAICETKFHY DKIRPI+YV++LSLNMQ YPPEDWLVGSSLPSKF+P 
Sbjct: 361 QSGICQWIFDELSAICETKFHYADKIRPINYVISLSLNMQLYPPEDWLVGSSLPSKFDPK 420

Query: 465 LIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKL 524
           LIG VLDQLSVDNVRIFWESK FEGKTDKVE+WYGTAYSIEKISG L+QDW+QSAPDV L
Sbjct: 421 LIGTVLDQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKISGHLVQDWMQSAPDVNL 480

Query: 525 HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA 584
           HLPASN+FIPTDLSLK ACEKVEFP+LLRKSSYS+IWYKPDTMF TPKAYVKIDF CPHA
Sbjct: 481 HLPASNIFIPTDLSLKRACEKVEFPVLLRKSSYSSIWYKPDTMFSTPKAYVKIDFICPHA 540

Query: 585 DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV 631
           DISPEAEVLT IFTRLLMDYLNEYAY A+VAGLYYGI+  DSGFQV
Sbjct: 541 DISPEAEVLTAIFTRLLMDYLNEYAYYAEVAGLYYGINLIDSGFQV 586

BLAST of Carg03529 vs. ExPASy TrEMBL
Match: A0A6J1DJ48 (insulin-degrading enzyme-like 1, peroxisomal isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021555 PE=3 SV=1)

HSP 1 Score: 1124.4 bits (2907), Expect = 0.0e+00
Identity = 537/586 (91.64%), Postives = 563/586 (96.08%), Query Frame = 0

Query: 45  MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG 104
           MAV KEQVTH+IVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDP+G
Sbjct: 1   MAVVKEQVTHEIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPKG 60

Query: 105 LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL 164
           LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVN DCFEEAL
Sbjct: 61  LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEEAL 120

Query: 165 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN 224
           DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHP+HKFSTGN
Sbjct: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHKFSTGN 180

Query: 225 WDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNN 284
           WDTLEVQPKAKGLDTRHEL++F++ NYSSN+MHLVVYAKENLDKVQSLVENIFQDIPN+N
Sbjct: 181 WDTLEVQPKAKGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQDIPNHN 240

Query: 285 CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 344
           CN A+FPGQPC SEHL+VLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG
Sbjct: 241 CNCAHFPGQPCTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 300

Query: 345 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK 404
           EGSLYYVLKTLGWATGLSAGESDST NFSFFKV IDLTD GQEHMQD+VGLLFKYI LLK
Sbjct: 301 EGSLYYVLKTLGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKYIDLLK 360

Query: 405 ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN 464
           +SGIC+WIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQ YPPEDWLVGSSLPSKFNPN
Sbjct: 361 QSGICKWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPSKFNPN 420

Query: 465 LIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKL 524
           LIG VLDQLSV+N RIFWESK FEG+TDKVE WYGT YSIE ISG LIQ+W+ SAPDVKL
Sbjct: 421 LIGTVLDQLSVENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSAPDVKL 480

Query: 525 HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA 584
           HLPA N+FIPTDLSLK A EKVEFP+LLRKSSYST+WYKPDTMFFTPKAYVKIDF+CPHA
Sbjct: 481 HLPAPNIFIPTDLSLKDAHEKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDFRCPHA 540

Query: 585 DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV 631
            ISPEAEVLT+IFTRLLMDYLNEYAYSAQVAGLYYGI+P ++GFQV
Sbjct: 541 GISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQV 586

BLAST of Carg03529 vs. TAIR 10
Match: AT2G41790.1 (Insulinase (Peptidase family M16) family protein )

HSP 1 Score: 915.2 bits (2364), Expect = 2.9e-266
Identity = 426/589 (72.33%), Postives = 505/589 (85.74%), Query Frame = 0

Query: 45  MAVGKEQVT---HDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSD 104
           MAV K   T    +I+KPRTD REYR IVL+N L+VLL+SDPDTDK AASM+V+VGSFSD
Sbjct: 1   MAVEKSNTTVGGVEILKPRTDNREYRMIVLKNLLQVLLISDPDTDKCAASMSVSVGSFSD 60

Query: 105 PEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFE 164
           P+GLEGLAHFLEHMLFYASEKYP EDSYSKYITEHGGS NA+TASE+TNY+F+VN DCF+
Sbjct: 61  PQGLEGLAHFLEHMLFYASEKYPEEDSYSKYITEHGGSTNAYTASEETNYHFDVNADCFD 120

Query: 165 EALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFS 224
           EALDRFAQFFIKPLMS DATMREIKAVDSENQKNLLSDGWR+ QLQ+H+S E HP+HKFS
Sbjct: 121 EALDRFAQFFIKPLMSADATMREIKAVDSENQKNLLSDGWRIRQLQKHLSKEDHPYHKFS 180

Query: 225 TGNWDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIP 284
           TGN DTL V+P+AKG+DTR EL+KFY+ +YS+N+MHLVVY KE+LDK+Q LVE +FQ+I 
Sbjct: 181 TGNMDTLHVRPQAKGVDTRSELIKFYEEHYSANIMHLVVYGKESLDKIQDLVERMFQEIQ 240

Query: 285 NNNCNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIG 344
           N N     FPGQPC ++HLQ+LV+AIPIKQGHKL + WP+TP IHHY E P +YLGHLIG
Sbjct: 241 NTNKVVPRFPGQPCTADHLQILVKAIPIKQGHKLGVSWPVTPSIHHYDEAPSQYLGHLIG 300

Query: 345 HEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYIS 404
           HEGEGSL++ LKTLGWATGLSAGE + T+++SFFKV IDLTD G EHMQ+++GLLF YI 
Sbjct: 301 HEGEGSLFHALKTLGWATGLSAGEGEWTLDYSFFKVSIDLTDAGHEHMQEILGLLFNYIQ 360

Query: 405 LLKESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKF 464
           LL+++G+CQWIFDELSAICETKFHYQDKI P+ Y+V+++ NMQ YP +DWLVGSSLP+KF
Sbjct: 361 LLQQTGVCQWIFDELSAICETKFHYQDKIPPMSYIVDIASNMQIYPTKDWLVGSSLPTKF 420

Query: 465 NPNLIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPD 524
           NP ++  V+D+LS  N RIFWES+ FEG+TDK E WY TAYS+EKI+   IQ+W+QSAPD
Sbjct: 421 NPAIVQKVVDELSPSNFRIFWESQKFEGQTDKAEPWYNTAYSLEKITSSTIQEWVQSAPD 480

Query: 525 VKLHLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKC 584
           V LHLPA NVFIPTDLSLK A +K   P+LLRK+ +S +WYKPDTMF  PKAYVK+DF C
Sbjct: 481 VHLHLPAPNVFIPTDLSLKDADDKETVPVLLRKTPFSRLWYKPDTMFSKPKAYVKMDFNC 540

Query: 585 PHADISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV 631
           P A  SP+A VLT+IFTRLLMDYLNEYAY AQVAGLYYG+S +D+GF++
Sbjct: 541 PLAVSSPDAAVLTDIFTRLLMDYLNEYAYYAQVAGLYYGVSLSDNGFEL 589

BLAST of Carg03529 vs. TAIR 10
Match: AT3G57470.1 (Insulinase (Peptidase family M16) family protein )

HSP 1 Score: 867.5 bits (2240), Expect = 6.9e-252
Identity = 413/591 (69.88%), Postives = 485/591 (82.06%), Query Frame = 0

Query: 45  MAVGKEQVT-----HDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSF 104
           MAVG E  T      +I+KPRTDKREYRRIVL+NSLEVLL+SDP+TDK AASM V+VGSF
Sbjct: 1   MAVGMENATASGECGEILKPRTDKREYRRIVLKNSLEVLLISDPETDKCAASMNVSVGSF 60

Query: 105 SDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDC 164
           +DPEGLEGLAHFLEHMLFYASEKYP EDSYSKYITEHGGS NA+T+SE TNY+F++N D 
Sbjct: 61  TDPEGLEGLAHFLEHMLFYASEKYPEEDSYSKYITEHGGSTNAYTSSEDTNYHFDINTDS 120

Query: 165 FEEALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHK 224
           F EALDRFAQFFI+PLMS DATMREIKAVDSE+Q NLLSD WRM QLQ+H+S E HP+HK
Sbjct: 121 FYEALDRFAQFFIQPLMSTDATMREIKAVDSEHQNNLLSDSWRMAQLQKHLSREDHPYHK 180

Query: 225 FSTGNWDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQD 284
           FSTGN DTL V+P+  G+DTR EL+KFY  +YS+N+MHLVVY KENLDK Q LVE +FQ 
Sbjct: 181 FSTGNMDTLHVRPEENGVDTRSELIKFYDEHYSANIMHLVVYGKENLDKTQGLVEALFQG 240

Query: 285 IPNNNCNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHL 344
           I N N     FPGQPC  +HLQVLV+A+PI QGH+L + WP+TP I HY+E PCRYLG L
Sbjct: 241 IRNTNQGIPRFPGQPCTLDHLQVLVKAVPIMQGHELSVSWPVTPSISHYEEAPCRYLGDL 300

Query: 345 IGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKY 404
           IGHEGEGSL++ LK LGWATGL AGE+D +M +SFF V IDLTD G EHMQD++GLLF+Y
Sbjct: 301 IGHEGEGSLFHALKILGWATGLYAGEADWSMEYSFFNVSIDLTDAGHEHMQDILGLLFEY 360

Query: 405 ISLLKESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPS 464
           I +L++SG+ QWIFDELSAICE +FHYQ KI PI Y V++S NM+ YP + WLVGSSLPS
Sbjct: 361 IKVLQQSGVSQWIFDELSAICEAEFHYQAKIDPISYAVDISSNMKIYPTKHWLVGSSLPS 420

Query: 465 KFNPNLIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSA 524
           KFNP ++  VLD+LS +NVRIFWES  FEG+TDKVE WY TAYS+EKI+   IQ+W+QSA
Sbjct: 421 KFNPAIVQKVLDELSPNNVRIFWESNKFEGQTDKVEPWYNTAYSLEKITKFTIQEWMQSA 480

Query: 525 PDVKLHLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDF 584
           PDV L LP  NVFIPTD SLK   +K  FP+LLRK+SYS +WYKPDT FF PKAYVK+DF
Sbjct: 481 PDVNLLLPTPNVFIPTDFSLKDLKDKDIFPVLLRKTSYSRLWYKPDTKFFKPKAYVKMDF 540

Query: 585 KCPHADISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV 631
            CP A  SP+A VL++IF  LL+DYLNEYAY AQ AGL YG+S +D+GF++
Sbjct: 541 NCPLAVSSPDAAVLSDIFVWLLVDYLNEYAYYAQAAGLDYGLSLSDNGFEL 591

BLAST of Carg03529 vs. TAIR 10
Match: AT3G57470.2 (Insulinase (Peptidase family M16) family protein )

HSP 1 Score: 760.4 bits (1962), Expect = 1.2e-219
Identity = 356/516 (68.99%), Postives = 421/516 (81.59%), Query Frame = 0

Query: 115 MLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEALDRFAQFFIKP 174
           MLFYASEKYP EDSYSKYITEHGGS NA+T+SE TNY+F++N D F EALDRFAQFFI+P
Sbjct: 1   MLFYASEKYPEEDSYSKYITEHGGSTNAYTSSEDTNYHFDINTDSFYEALDRFAQFFIQP 60

Query: 175 LMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGNWDTLEVQPKA 234
           LMS DATMREIKAVDSE+Q NLLSD WRM QLQ+H+S E HP+HKFSTGN DTL V+P+ 
Sbjct: 61  LMSTDATMREIKAVDSEHQNNLLSDSWRMAQLQKHLSREDHPYHKFSTGNMDTLHVRPEE 120

Query: 235 KGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNNCNRANFPGQP 294
            G+DTR EL+KFY  +YS+N+MHLVVY KENLDK Q LVE +FQ I N N     FPGQP
Sbjct: 121 NGVDTRSELIKFYDEHYSANIMHLVVYGKENLDKTQGLVEALFQGIRNTNQGIPRFPGQP 180

Query: 295 CMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEGEGSLYYVLKT 354
           C  +HLQVLV+A+PI QGH+L + WP+TP I HY+E PCRYLG LIGHEGEGSL++ LK 
Sbjct: 181 CTLDHLQVLVKAVPIMQGHELSVSWPVTPSISHYEEAPCRYLGDLIGHEGEGSLFHALKI 240

Query: 355 LGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLKESGICQWIFD 414
           LGWATGL AGE+D +M +SFF V IDLTD G EHMQD++GLLF+YI +L++SG+ QWIFD
Sbjct: 241 LGWATGLYAGEADWSMEYSFFNVSIDLTDAGHEHMQDILGLLFEYIKVLQQSGVSQWIFD 300

Query: 415 ELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPNLIGMVLDQLS 474
           ELSAICE +FHYQ KI PI Y V++S NM+ YP + WLVGSSLPSKFNP ++  VLD+LS
Sbjct: 301 ELSAICEAEFHYQAKIDPISYAVDISSNMKIYPTKHWLVGSSLPSKFNPAIVQKVLDELS 360

Query: 475 VDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKLHLPASNVFIP 534
            +NVRIFWES  FEG+TDKVE WY TAYS+EKI+   IQ+W+QSAPDV L LP  NVFIP
Sbjct: 361 PNNVRIFWESNKFEGQTDKVEPWYNTAYSLEKITKFTIQEWMQSAPDVNLLLPTPNVFIP 420

Query: 535 TDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHADISPEAEVLT 594
           TD SLK   +K  FP+LLRK+SYS +WYKPDT FF PKAYVK+DF CP A  SP+A VL+
Sbjct: 421 TDFSLKDLKDKDIFPVLLRKTSYSRLWYKPDTKFFKPKAYVKMDFNCPLAVSSPDAAVLS 480

Query: 595 EIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV 631
           +IF  LL+DYLNEYAY AQ AGL YG+S +D+GF++
Sbjct: 481 DIFVWLLVDYLNEYAYYAQAAGLDYGLSLSDNGFEL 516

BLAST of Carg03529 vs. TAIR 10
Match: AT3G57470.3 (Insulinase (Peptidase family M16) family protein )

HSP 1 Score: 760.4 bits (1962), Expect = 1.2e-219
Identity = 356/516 (68.99%), Postives = 421/516 (81.59%), Query Frame = 0

Query: 115 MLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEALDRFAQFFIKP 174
           MLFYASEKYP EDSYSKYITEHGGS NA+T+SE TNY+F++N D F EALDRFAQFFI+P
Sbjct: 1   MLFYASEKYPEEDSYSKYITEHGGSTNAYTSSEDTNYHFDINTDSFYEALDRFAQFFIQP 60

Query: 175 LMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGNWDTLEVQPKA 234
           LMS DATMREIKAVDSE+Q NLLSD WRM QLQ+H+S E HP+HKFSTGN DTL V+P+ 
Sbjct: 61  LMSTDATMREIKAVDSEHQNNLLSDSWRMAQLQKHLSREDHPYHKFSTGNMDTLHVRPEE 120

Query: 235 KGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNNCNRANFPGQP 294
            G+DTR EL+KFY  +YS+N+MHLVVY KENLDK Q LVE +FQ I N N     FPGQP
Sbjct: 121 NGVDTRSELIKFYDEHYSANIMHLVVYGKENLDKTQGLVEALFQGIRNTNQGIPRFPGQP 180

Query: 295 CMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEGEGSLYYVLKT 354
           C  +HLQVLV+A+PI QGH+L + WP+TP I HY+E PCRYLG LIGHEGEGSL++ LK 
Sbjct: 181 CTLDHLQVLVKAVPIMQGHELSVSWPVTPSISHYEEAPCRYLGDLIGHEGEGSLFHALKI 240

Query: 355 LGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLKESGICQWIFD 414
           LGWATGL AGE+D +M +SFF V IDLTD G EHMQD++GLLF+YI +L++SG+ QWIFD
Sbjct: 241 LGWATGLYAGEADWSMEYSFFNVSIDLTDAGHEHMQDILGLLFEYIKVLQQSGVSQWIFD 300

Query: 415 ELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPNLIGMVLDQLS 474
           ELSAICE +FHYQ KI PI Y V++S NM+ YP + WLVGSSLPSKFNP ++  VLD+LS
Sbjct: 301 ELSAICEAEFHYQAKIDPISYAVDISSNMKIYPTKHWLVGSSLPSKFNPAIVQKVLDELS 360

Query: 475 VDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKLHLPASNVFIP 534
            +NVRIFWES  FEG+TDKVE WY TAYS+EKI+   IQ+W+QSAPDV L LP  NVFIP
Sbjct: 361 PNNVRIFWESNKFEGQTDKVEPWYNTAYSLEKITKFTIQEWMQSAPDVNLLLPTPNVFIP 420

Query: 535 TDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHADISPEAEVLT 594
           TD SLK   +K  FP+LLRK+SYS +WYKPDT FF PKAYVK+DF CP A  SP+A VL+
Sbjct: 421 TDFSLKDLKDKDIFPVLLRKTSYSRLWYKPDTKFFKPKAYVKMDFNCPLAVSSPDAAVLS 480

Query: 595 EIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQV 631
           +IF  LL+DYLNEYAY AQ AGL YG+S +D+GF++
Sbjct: 481 DIFVWLLVDYLNEYAYYAQAAGLDYGLSLSDNGFEL 516

BLAST of Carg03529 vs. TAIR 10
Match: AT1G06900.1 (Insulinase (Peptidase family M16) family protein )

HSP 1 Score: 358.6 bits (919), Expect = 1.0e-98
Identity = 211/559 (37.75%), Postives = 310/559 (55.46%), Query Frame = 0

Query: 76  EVLLVSDPDTDKSAASMTVNVGSFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITE 135
           EV    D  T K+AA+M V++GSF DP   +GLAHFLEHMLF  S ++P E+ Y  Y+++
Sbjct: 95  EVKGKGDHQTKKAAAAMCVSMGSFLDPPEAQGLAHFLEHMLFMGSTEFPDENEYDSYLSK 154

Query: 136 HGGSMNAFTASEQTNYYFEVNVDCFEEALDRFAQFFIKPLMSPDATMREIKAVDSENQKN 195
           HGGS NA+T  E T Y+FEV  +  + AL RF+QFF+ PLM  +A  RE+ AVDSE  + 
Sbjct: 155 HGGSSNAYTEMEHTCYHFEVKREFLQGALKRFSQFFVAPLMKTEAMEREVLAVDSEFNQA 214

Query: 196 LLSDGWRMHQLQRHISSESHPFHKFSTGNWDTLEVQPKAKGLDTRHELLKFYKNNYSSNV 255
           L +D  R+ QLQ + S++ HPF++F+ GN  +L       G+D R  ++K YK  Y   +
Sbjct: 215 LQNDACRLQQLQCYTSAKGHPFNRFAWGNKKSLS-GAMENGVDLRECIVKLYKEYYHGGL 274

Query: 256 MHLVVYAKENLDKVQSLVENIFQDIPNNNCNRANFPGQPCMSEHLQVLVRAIPIKQGHKL 315
           M LVV   E+LD ++S V  +F D+ N +  R     +  + +  + L R   +K  H L
Sbjct: 275 MKLVVIGGESLDMLESWVVELFGDVKNGSKIRPTLEAEGPIWKGGK-LYRLEAVKDVHIL 334

Query: 316 RIVWPITPEIHHYKEGPCRYLGHLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFS-- 375
            + W + P    Y + P  YL HL+GHEG GSL+  LK  GWAT LSAG  D  +N S  
Sbjct: 335 DLTWTLPPLRSAYVKKPEDYLAHLLGHEGRGSLHSFLKAKGWATSLSAGVGDDGINRSSL 394

Query: 376 --FFKVGIDLTDVGQEHMQDVVGLLFKYISLLKESGICQWIFDELSAICETKFHYQDKIR 435
              F + I LTD G E + D++G +++Y+ LL++    +WIF EL  I    F + ++  
Sbjct: 395 AYVFGMSIHLTDSGLEKIYDIIGYIYQYLKLLRDVSPQEWIFKELQDIGNMDFRFAEEQP 454

Query: 436 PIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPNLIGMVLDQLSVDNVRIFWESKNFEGKT 495
             DY   LS NM  YP E  + G  +   ++P LI  ++   +  N+RI   SK+ + + 
Sbjct: 455 ADDYAAELSENMLAYPVEHVIYGDYVYQTWDPKLIEDLMGFFTPQNMRIDVVSKSIKSEE 514

Query: 496 DKVEQWYGTAYSIEKISGPLIQDWIQ-SAPDVKLHLPASNVFIPTDLSLKGACEKVE--- 555
            + E W+G++Y  E +   L++ W   S  D  LHLP+ N FIP D S++     V+   
Sbjct: 515 FQQEPWFGSSYIEEDVPLSLMESWSNPSEVDNSLHLPSKNQFIPCDFSIRAINSDVDPKS 574

Query: 556 --FPILLRKSSYSTIWYKPDTMFFTPKA--YVKIDFKCPHADISPEAEVLTEIFTRLLMD 615
              P  +    +   WYK D  F  P+A  Y +I+ K  +A +  +  +LTE++  LL D
Sbjct: 575 QSPPRCIIDEPFMKFWYKLDETFKVPRANTYFRINLKGAYASV--KNCLLTELYINLLKD 634

Query: 616 YLNEYAYSAQVAGLYYGIS 623
            LNE  Y A +A L   +S
Sbjct: 635 ELNEIIYQASIAKLETSLS 649

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7017597.10.0e+00100.00Insulin-degrading enzyme-like 1, peroxisomal, partial [Cucurbita argyrosperma su... [more]
KAG6580840.10.0e+00100.00Insulin-degrading enzyme-like 1, peroxisomal, partial [Cucurbita argyrosperma su... [more]
XP_023526970.10.0e+00100.00insulin-degrading enzyme-like 1, peroxisomal [Cucurbita pepo subsp. pepo][more]
XP_022934728.10.0e+0099.83insulin-degrading enzyme-like 1, peroxisomal [Cucurbita moschata][more]
XP_022982829.10.0e+0099.49insulin-degrading enzyme-like 1, peroxisomal [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O229414.1e-26572.33Insulin-degrading enzyme-like 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=... [more]
F4J3D99.7e-25169.88Insulin-degrading enzyme-like 2 OS=Arabidopsis thaliana OX=3702 GN=At3g57470 PE=... [more]
Q24K021.6e-14443.80Insulin-degrading enzyme OS=Bos taurus OX=9913 GN=IDE PE=2 SV=1[more]
P355596.1e-14443.90Insulin-degrading enzyme OS=Rattus norvegicus OX=10116 GN=Ide PE=1 SV=1[more]
Q9JHR77.9e-14443.63Insulin-degrading enzyme OS=Mus musculus OX=10090 GN=Ide PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F8G90.0e+0099.83insulin-degrading enzyme-like 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1J5M20.0e+0099.49insulin-degrading enzyme-like 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A1S3B7B50.0e+0091.81insulin-degrading enzyme-like 1, peroxisomal isoform X1 OS=Cucumis melo OX=3656 ... [more]
A0A5A7TLF90.0e+0091.81Insulin-degrading enzyme-like 1, peroxisomal isoform X1 OS=Cucumis melo var. mak... [more]
A0A6J1DJ480.0e+0091.64insulin-degrading enzyme-like 1, peroxisomal isoform X1 OS=Momordica charantia O... [more]
Match NameE-valueIdentityDescription
AT2G41790.12.9e-26672.33Insulinase (Peptidase family M16) family protein [more]
AT3G57470.16.9e-25269.88Insulinase (Peptidase family M16) family protein [more]
AT3G57470.21.2e-21968.99Insulinase (Peptidase family M16) family protein [more]
AT3G57470.31.2e-21968.99Insulinase (Peptidase family M16) family protein [more]
AT1G06900.11.0e-9837.75Insulinase (Peptidase family M16) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.830.10coord: 530..633
e-value: 1.8E-31
score: 111.3
NoneNo IPR availableGENE3D3.30.830.10coord: 288..516
e-value: 4.7E-93
score: 312.7
NoneNo IPR availableGENE3D3.30.830.10coord: 41..286
e-value: 2.5E-89
score: 301.0
NoneNo IPR availablePANTHERPTHR43690NARDILYSINcoord: 52..631
NoneNo IPR availablePANTHERPTHR43690:SF27ENZYME, PUTATIVE-RELATEDcoord: 52..631
IPR007863Peptidase M16, C-terminalPFAMPF05193Peptidase_M16_Ccoord: 239..415
e-value: 1.1E-20
score: 74.4
IPR032632Peptidase M16, middle/third domainPFAMPF16187Peptidase_M16_Mcoord: 424..630
e-value: 4.6E-71
score: 239.3
IPR011765Peptidase M16, N-terminalPFAMPF00675Peptidase_M16coord: 77..211
e-value: 4.5E-39
score: 133.8
IPR001431Peptidase M16, zinc-binding sitePROSITEPS00143INSULINASEcoord: 97..120
IPR011249Metalloenzyme, LuxS/M16 peptidase-likeSUPERFAMILY63411LuxS/MPP-like metallohydrolasecoord: 55..284
IPR011249Metalloenzyme, LuxS/M16 peptidase-likeSUPERFAMILY63411LuxS/MPP-like metallohydrolasecoord: 292..528
IPR011249Metalloenzyme, LuxS/M16 peptidase-likeSUPERFAMILY63411LuxS/MPP-like metallohydrolasecoord: 530..630

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg03529-RACarg03529-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043171 peptide catabolic process
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0005829 cytosol
cellular_component GO:0005739 mitochondrion
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004222 metalloendopeptidase activity