Carg03418 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg03418
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionRibosomal RNA large subunit methyltransferase I
LocationCarg_Chr20: 1437283 .. 1439724 (+)
RNA-Seq ExpressionCarg03418
SyntenyCarg03418
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGAAACTGATTGAAACCAGGATACTTACTGCTAGAGAATTAAGAAAGAGCTTGGGACTTCCGTCGGCCAGTACGAATGCATACCGTCTCGTTAATAGTGAAGGAGATAGGTATCGACCTCGAGTATTCTGTTCATGTCTTCATACATTCTGTAAAACTTGATTAAATGTAGTACCACCATGCATTGATCTTCGTTCTTAAGCATTTCAAAAGTCATTCCAAACCGACCCAACTCTAACTGTTTGCGTGTATGAATCTCTTAGAGTTTGTTTGTGTACTGGAACCTATAATAGCCTTATCCAAATGTCTTAGAATTATTGTTTTTTTTTTTTTTTCTTTTCTTTTTTTGGCAGATTGTCAGGACTAATCATTGACGTCTTTGGAGATTTAGCAGTTGTGGCATCATCGGCTGCTTGGGTGGAGAAGTACAAAGCTGAAATCGAGGATTGTGTTCGTGGAATTGATGAAATCAATCATGTGAAATGGAGACCATCGGTGGAAATTCTGAAGGAAGAAGGGATAGATGTGTCTGAATTGAAGGATACAAATCCATCTAATTGTCCTGAAAGAACAAAGGTAACAAGCGACTGCAACACTTGTACTGTTTCAAGAAATGCTCTATATCATGTTCATGGAAACTAAATGTTAAAAATGTGGAGCCCTTTTGATGAGTAAACTGATCTTACATGCTTGAATAATCATCAATGAGTATCAAAGATTTGGAAACTAGGAGATAAGAGTTGCTTATATAAGTAAGCTTTATGCACAAATCCTCACTGGGATGTGTTCTAGCAGGTGGAAATTCTTGGTAACAAGAGAAGCTATGAGGGTATCATGTTAATGTAGAAAGTACTTCAAAAGCTATATAATACATACAACCAAGTTATTACCACAAAATCAGATGAGATATTGAACATGCCTTACTCTGATAGGTAACAGAGAATGGAATTTTCTATGCCATTTCACTTGAAGGCCAGAAAACAGGGTTTTATGCTGATCAGCGTGAAAATCGTCATTTTATCTCAACAATTTCTGAGGGTCAAAAAGTTCTTGACCTCTGCTGCTACAGTGGTGGGTTTTCTCTTAATGCAGCACGTGGTGGAGCTATTAATGTCACCGGTATACATTTCTTTTCTTGTCTTCTATTAAAGCTTTTAGATCAAATGTTTAGGAGAGTATGCTGTGGCAAAGTCACTAATTATTCATACTTATAAAGAAATTCAATCTTTTCTGTGTGTTTATCTGTTGCTATCACGGAAAAGAAACAGGATTCTCTAGCCTTGAATACAGTGGTACTATTCTCATAAGTTCGCTAATTATCAGGAATTGATTCATCACTGCCTGCTCTGGAGCTAGCAAAAGAAAATGTAATCCTTAATAGTATGGATCCTGACAGAATATCATTTTTGAAAGCTGATGCCACTACATTCATGAAGGATGCTCTATCTAGGAATGAATGTTGGGATATAGTCATTTTGGATCCTCCCAAACTAGCTCCACGAAAAAAGGTATGATGCATTTGATTATTTTTCCTGTTACGAAAGTTCTTTGATTAATTAATTCTATGATCTTACATGTTAGGCCCCATGTGAAATACTGGTACAAAGTGAACATAAGTTCTTAATTCTTTGATTCTGTTCACTCTTACAAGTTAGAATCCACGTGAAATCCTGTCATCATATCTTAAACGTCAGAAAGTTGTGGATTTTAAGATGATAGATCAAATTGTTCAATAAATCATCCTGGGCTTGTTTTGAAAGTATCAAGTTTGTTAATTTTCATCATCCTACTAACTAAAGCATAGTAGGTTCTACAAACTGCATCAGGCATGTACAGGACTTTAAATTCATTAGCAATGCAACTCACAAGGAAAGGTGGTCTTCTCATGACTTGTTCATGCTCTGGAGCAATGACTCAGAGTGGGATGTTCCTGCAAACACTCCAGGCAAGTCCAGTTCCGCCCCCACCAACCATGAAAATAGATCATTACTATTGCTTATTTCAATGACTTAGAAACATTGTTGGGTAATTATGTAGTTTCTATGCCTCATCATCTTGACTTAAACTATATGAAGAGTTATTTAGGATAATATCTATCGTGTCATGTACGAAAAGAATCTATTCAGCTTGTTTAAGGAAACTAAACTGGTATGTCCTGCCCCTTTCATTTAAACAGGGTGCAGCATCAATGGCCAAAAAGAAAATCACGGTCCTACGACAAGCAGGAGCAGCATGTGATCACCCCATTGACCCTACTTACCCAGAGGGTGCATATCTTTCTAATATCCTTCTACGTGTGCTCTAAACTTGATGAAGTTATAAATGTATCTACCACTGATCAAAAGAGACAAGCCATTTAACATAAGGAGATGAGATACTGCATTTGAAGTTACACTATCGTCAGAGCCACATCTTCATATAAAAAGGAAAGTAGAAGTT

mRNA sequence

ATGCAGAAACTGATTGAAACCAGGATACTTACTGCTAGAGAATTAAGAAAGAGCTTGGGACTTCCGTCGGCCAGTACGAATGCATACCGTCTCGTTAATAGTGAAGGAGATAGATTGTCAGGACTAATCATTGACGTCTTTGGAGATTTAGCAGTTGTGGCATCATCGGCTGCTTGGGTGGAGAAGTACAAAGCTGAAATCGAGGATTGTGTTCGTGGAATTGATGAAATCAATCATGTGAAATGGAGACCATCGGTGGAAATTCTGAAGGAAGAAGGGATAGATGTGTCTGAATTGAAGGATACAAATCCATCTAATTGTCCTGAAAGAACAAAGGTAACAGAGAATGGAATTTTCTATGCCATTTCACTTGAAGGCCAGAAAACAGGGTTTTATGCTGATCAGCGTGAAAATCGTCATTTTATCTCAACAATTTCTGAGGGTCAAAAAGTTCTTGACCTCTGCTGCTACAGTGGTGGGTTTTCTCTTAATGCAGCACGTGGTGGAGCTATTAATGTCACCGGAATTGATTCATCACTGCCTGCTCTGGAGCTAGCAAAAGAAAATGTAATCCTTAATAGTATGGATCCTGACAGAATATCATTTTTGAAAGCTGATGCCACTACATTCATGAAGGATGCTCTATCTAGGAATGAATGTTGGGATATAGTCATTTTGGATCCTCCCAAACTAGCTCCACGAAAAAAGGTTCTACAAACTGCATCAGGCATGTACAGGACTTTAAATTCATTAGCAATGCAACTCACAAGGAAAGGTGGTCTTCTCATGACTTGTTCATGCTCTGGAGCAATGACTCAGAGTGGGATGTTCCTGCAAACACTCCAGGCAAGTCCAGGTGCAGCATCAATGGCCAAAAAGAAAATCACGGTCCTACGACAAGCAGGAGCAGCATGTGATCACCCCATTGACCCTACTTACCCAGAGGGTGCATATCTTTCTAATATCCTTCTACGTGTGCTCTAAACTTGATGAAGTTATAAATGTATCTACCACTGATCAAAAGAGACAAGCCATTTAACATAAGGAGATGAGATACTGCATTTGAAGTTACACTATCGTCAGAGCCACATCTTCATATAAAAAGGAAAGTAGAAGTT

Coding sequence (CDS)

ATGCAGAAACTGATTGAAACCAGGATACTTACTGCTAGAGAATTAAGAAAGAGCTTGGGACTTCCGTCGGCCAGTACGAATGCATACCGTCTCGTTAATAGTGAAGGAGATAGATTGTCAGGACTAATCATTGACGTCTTTGGAGATTTAGCAGTTGTGGCATCATCGGCTGCTTGGGTGGAGAAGTACAAAGCTGAAATCGAGGATTGTGTTCGTGGAATTGATGAAATCAATCATGTGAAATGGAGACCATCGGTGGAAATTCTGAAGGAAGAAGGGATAGATGTGTCTGAATTGAAGGATACAAATCCATCTAATTGTCCTGAAAGAACAAAGGTAACAGAGAATGGAATTTTCTATGCCATTTCACTTGAAGGCCAGAAAACAGGGTTTTATGCTGATCAGCGTGAAAATCGTCATTTTATCTCAACAATTTCTGAGGGTCAAAAAGTTCTTGACCTCTGCTGCTACAGTGGTGGGTTTTCTCTTAATGCAGCACGTGGTGGAGCTATTAATGTCACCGGAATTGATTCATCACTGCCTGCTCTGGAGCTAGCAAAAGAAAATGTAATCCTTAATAGTATGGATCCTGACAGAATATCATTTTTGAAAGCTGATGCCACTACATTCATGAAGGATGCTCTATCTAGGAATGAATGTTGGGATATAGTCATTTTGGATCCTCCCAAACTAGCTCCACGAAAAAAGGTTCTACAAACTGCATCAGGCATGTACAGGACTTTAAATTCATTAGCAATGCAACTCACAAGGAAAGGTGGTCTTCTCATGACTTGTTCATGCTCTGGAGCAATGACTCAGAGTGGGATGTTCCTGCAAACACTCCAGGCAAGTCCAGGTGCAGCATCAATGGCCAAAAAGAAAATCACGGTCCTACGACAAGCAGGAGCAGCATGTGATCACCCCATTGACCCTACTTACCCAGAGGGTGCATATCTTTCTAATATCCTTCTACGTGTGCTCTAA

Protein sequence

MQKLIETRILTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFYAISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSLPALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQTASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQASPGAASMAKKKITVLRQAGAACDHPIDPTYPEGAYLSNILLRVL
Homology
BLAST of Carg03418 vs. NCBI nr
Match: KAG7010450.1 (rlmI, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 645.2 bits (1663), Expect = 3.0e-181
Identity = 327/327 (100.00%), Postives = 327/327 (100.00%), Query Frame = 0

Query: 1   MQKLIETRILTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV 60
           MQKLIETRILTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV
Sbjct: 1   MQKLIETRILTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV 60

Query: 61  EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFY 120
           EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFY
Sbjct: 61  EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFY 120

Query: 121 AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL 180
           AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL
Sbjct: 121 AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL 180

Query: 181 PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT 240
           PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT
Sbjct: 181 PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT 240

Query: 241 ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQASPGAASMAKKKITVLRQ 300
           ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQASPGAASMAKKKITVLRQ
Sbjct: 241 ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQASPGAASMAKKKITVLRQ 300

Query: 301 AGAACDHPIDPTYPEGAYLSNILLRVL 328
           AGAACDHPIDPTYPEGAYLSNILLRVL
Sbjct: 301 AGAACDHPIDPTYPEGAYLSNILLRVL 327

BLAST of Carg03418 vs. NCBI nr
Match: KAG6570599.1 (hypothetical protein SDJN03_29514, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 634.4 bits (1635), Expect = 5.4e-178
Identity = 324/327 (99.08%), Postives = 324/327 (99.08%), Query Frame = 0

Query: 1   MQKLIETRILTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV 60
           MQKLIETRILTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV
Sbjct: 116 MQKLIETRILTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV 175

Query: 61  EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFY 120
           EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFY
Sbjct: 176 EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFY 235

Query: 121 AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL 180
           AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL
Sbjct: 236 AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL 295

Query: 181 PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT 240
           PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT
Sbjct: 296 PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT 355

Query: 241 ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQASPGAASMAKKKITVLRQ 300
           ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQ   GAASMAKKKITVLRQ
Sbjct: 356 ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQ---GAASMAKKKITVLRQ 415

Query: 301 AGAACDHPIDPTYPEGAYLSNILLRVL 328
           AGAACDHPIDPTYPEGAYLSNILLRVL
Sbjct: 416 AGAACDHPIDPTYPEGAYLSNILLRVL 439

BLAST of Carg03418 vs. NCBI nr
Match: XP_022985853.1 (uncharacterized protein LOC111483780 [Cucurbita maxima])

HSP 1 Score: 627.9 bits (1618), Expect = 5.0e-176
Identity = 321/327 (98.17%), Postives = 322/327 (98.47%), Query Frame = 0

Query: 1   MQKLIETRILTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV 60
           MQKLIETRI  ARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV
Sbjct: 116 MQKLIETRIHIARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV 175

Query: 61  EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFY 120
           EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFY
Sbjct: 176 EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFY 235

Query: 121 AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL 180
           AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL
Sbjct: 236 AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL 295

Query: 181 PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT 240
           PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT
Sbjct: 296 PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT 355

Query: 241 ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQASPGAASMAKKKITVLRQ 300
           ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQ   GAASMAKK+ITVLRQ
Sbjct: 356 ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQ---GAASMAKKQITVLRQ 415

Query: 301 AGAACDHPIDPTYPEGAYLSNILLRVL 328
           AGAACDHPIDPTYPEGAYLSNILLRVL
Sbjct: 416 AGAACDHPIDPTYPEGAYLSNILLRVL 439

BLAST of Carg03418 vs. NCBI nr
Match: XP_022943486.1 (uncharacterized protein LOC111448241 [Cucurbita moschata])

HSP 1 Score: 624.4 bits (1609), Expect = 5.6e-175
Identity = 321/327 (98.17%), Postives = 321/327 (98.17%), Query Frame = 0

Query: 1   MQKLIETRILTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV 60
           MQKLIETRI TARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV
Sbjct: 116 MQKLIETRIHTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV 175

Query: 61  EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFY 120
           EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPS C ERTKVTENGIFY
Sbjct: 176 EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSICLERTKVTENGIFY 235

Query: 121 AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL 180
           AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL
Sbjct: 236 AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL 295

Query: 181 PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT 240
           PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT
Sbjct: 296 PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT 355

Query: 241 ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQASPGAASMAKKKITVLRQ 300
           ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQ   GAASMAKKKITVLRQ
Sbjct: 356 ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQ---GAASMAKKKITVLRQ 415

Query: 301 AGAACDHPIDPTYPEGAYLSNILLRVL 328
           AGAACDHPIDPTYPEGAYLSNILLRVL
Sbjct: 416 AGAACDHPIDPTYPEGAYLSNILLRVL 439

BLAST of Carg03418 vs. NCBI nr
Match: XP_023512212.1 (uncharacterized protein LOC111777007 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 619.0 bits (1595), Expect = 2.3e-173
Identity = 319/327 (97.55%), Postives = 320/327 (97.86%), Query Frame = 0

Query: 1   MQKLIETRILTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV 60
           MQKLIETRI TARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV
Sbjct: 116 MQKLIETRIHTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV 175

Query: 61  EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFY 120
           EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPS  PERTKVTENGIFY
Sbjct: 176 EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSIYPERTKVTENGIFY 235

Query: 121 AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL 180
           AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL
Sbjct: 236 AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL 295

Query: 181 PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT 240
           PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNE WDIVILDPPKLAPRKKVLQT
Sbjct: 296 PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNESWDIVILDPPKLAPRKKVLQT 355

Query: 241 ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQASPGAASMAKKKITVLRQ 300
           ASGMYRTLNSLAMQLT+KGGLLMTCSCSGAMTQSGMFLQTLQ   GAASMAKKKITVLRQ
Sbjct: 356 ASGMYRTLNSLAMQLTKKGGLLMTCSCSGAMTQSGMFLQTLQ---GAASMAKKKITVLRQ 415

Query: 301 AGAACDHPIDPTYPEGAYLSNILLRVL 328
           AGAACDHPIDPTYPEGAYLSNILLRVL
Sbjct: 416 AGAACDHPIDPTYPEGAYLSNILLRVL 439

BLAST of Carg03418 vs. ExPASy Swiss-Prot
Match: B4RRY8 (Ribosomal RNA large subunit methyltransferase I OS=Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype) OX=1774373 GN=rlmI PE=3 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 8.7e-46
Identity = 119/321 (37.07%), Postives = 181/321 (56.39%), Query Frame = 0

Query: 8   RILTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKAEI 67
           R+ TA  LRK L  P+  TNA+R + SE D L G+ ID++ ++AVV   +A  EK++ +I
Sbjct: 82  RLETALALRKRLFDPN-KTNAFRWIASESDGLPGVTIDLYDNVAVVQLLSAGGEKHRDKI 141

Query: 68  EDCVRGIDEINHVKWRPSVEILKEEGID-VSELKDTNPSNCPERTKVTENGIFYAISLE- 127
              +  +    HV  R  V++ K+EG++ V+ +    P   P +  V ENGI   + +E 
Sbjct: 142 VWAITKLMPDVHVYERSDVDVRKKEGLEPVTGVLHGQP---PTQVTVKENGINIVVDIES 201

Query: 128 GQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSLPALEL 187
           G KTGFY DQR++R   +  ++   VL+   Y+G FS  A  GGA +VT +D S PAL+L
Sbjct: 202 GHKTGFYLDQRDSRAAAAHYAKDADVLNCFSYTGTFSCYALSGGAKSVTNVDVSQPALDL 261

Query: 188 AKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQTASGMY 247
           AK +V +N  D  R  +L  D    +++   +N+ +D+VILDPPK    K  L  A+  Y
Sbjct: 262 AKHHVAINGFDDKRTQYLNKDVFKALREYHEQNKQFDMVILDPPKFVDNKASLNRAARGY 321

Query: 248 RTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQASPGAASMAKKKITVLRQAGAAC 307
           + +N   +   + GGLL+T SCSG M  + +F + +     AA  A + I ++ +   A 
Sbjct: 322 KDINMYGIHAVKSGGLLLTFSCSGLM-PADLFQKVV---ADAALDAGRTIKIIARLNQAS 381

Query: 308 DHPIDPTYPEGAYLSNILLRV 327
           DHPI  +YPEG YL  ++  V
Sbjct: 382 DHPIIGSYPEGYYLKGLVCEV 394

BLAST of Carg03418 vs. ExPASy Swiss-Prot
Match: Q3IKJ0 (Ribosomal RNA large subunit methyltransferase I OS=Pseudoalteromonas translucida (strain TAC 125) OX=326442 GN=rlmI PE=3 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.6e-44
Identity = 114/327 (34.86%), Postives = 173/327 (52.91%), Query Frame = 0

Query: 2   QKLIETRILTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVE 61
           Q   E R+  A E R  + +       +RL  +E D L G+ ID F ++ V    +A  E
Sbjct: 76  QHFFERRLRRALEARSQV-IEEGGLTGFRLCAAESDFLPGVTIDKFDNVLVCQLLSAGAE 135

Query: 62  KYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPER-TKVTENGIFY 121
           ++K EI   +  I     +  R  VE+  +EG++   +K     N P     + ENG   
Sbjct: 136 RHKGEIVGALMAIFPGMSIYERSDVEVRTKEGLE--PIKGALWGNEPTAPVLIAENGFKI 195

Query: 122 AIS-LEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSS 181
            +  ++G KTGFY DQR++R  +   S+ + VL+   Y+G FSL A RGG  +VT +D S
Sbjct: 196 EVDIIDGHKTGFYLDQRDSRAALERFSKDKTVLNCFSYTGTFSLYALRGGCKHVTNVDVS 255

Query: 182 LPALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQ 241
            PAL+ AK NV  N++D D++ F+K D    ++        +D +++DPPK A  K  L 
Sbjct: 256 QPALDTAKRNVEHNNLDLDKVDFVKQDVFKLLRQYREDGVLFDTIVMDPPKFADNKAQLT 315

Query: 242 TASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQASPGAASMAKKKITVLR 301
            A   Y+ +N +AMQ+ + GG L+T SCSG M Q+    Q + A   AA  A K + ++ 
Sbjct: 316 GACRGYKDINMIAMQILKPGGTLLTFSCSGLMEQN--LFQKVVAD--AALDAGKDLLIME 375

Query: 302 QAGAACDHPIDPTYPEGAYLSNILLRV 327
           +   A DHPI  +YPEG YL  ++ +V
Sbjct: 376 RLNQAADHPIAGSYPEGFYLKGLICKV 395

BLAST of Carg03418 vs. ExPASy Swiss-Prot
Match: B2VDG5 (Ribosomal RNA large subunit methyltransferase I OS=Erwinia tasmaniensis (strain DSM 17950 / CFBP 7177 / CIP 109463 / NCPPB 4357 / Et1/99) OX=465817 GN=rlmI PE=3 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.4e-43
Identity = 109/324 (33.64%), Postives = 179/324 (55.25%), Query Frame = 0

Query: 8   RILTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKAEI 67
           R+  A++LR  L L   + ++YRL+  E D L G+ ID FG+  V+   +A  E  +A +
Sbjct: 82  RLNAAQQLRDWLALRD-NLDSYRLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAAL 141

Query: 68  EDCVRGIDEINHVKWRPSVEILKEEGIDVSE---LKDTNPSNCPERTKVTENGIFYAISL 127
              ++       +  R  V + K+EG+++++   L D  P   P    +TE+G+   + +
Sbjct: 142 ITALQRCYPDCAIYDRSDVSVRKKEGLELTQGVVLGDAPPPLLP----ITEHGMKLLVDI 201

Query: 128 E-GQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSLPAL 187
           + G KTG+Y DQR++R      ++G++VL+   Y+GGF+++A  G    V  +D+S  AL
Sbjct: 202 QTGHKTGYYLDQRDSRQATRRYAQGRRVLNCFSYTGGFAVSALMGNCKEVISVDTSQAAL 261

Query: 188 ELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQTASG 247
           ++A++NV LN +D  +  F + D    ++      E +D++I+DPPK    K  L  A  
Sbjct: 262 DVARQNVELNGLDVSKAHFQRDDVFKLLRRYRDEGEKFDLIIMDPPKFVENKNQLMGACR 321

Query: 248 MYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQASPGAASMAKKKITVLRQAGA 307
            Y+ +N LAMQL   GG+LMT SCSG M  + +F + L     AA  A++++  + Q   
Sbjct: 322 GYKDINMLAMQLLNPGGMLMTFSCSGLMA-TDLFQKIL---ADAAVDAQREVQFIEQFRQ 381

Query: 308 ACDHPIDPTYPEGAYLSNILLRVL 328
           A DHP+  +YPEG YL     RV+
Sbjct: 382 AADHPVISSYPEGMYLKGFACRVI 396

BLAST of Carg03418 vs. ExPASy Swiss-Prot
Match: B1LJ30 (Ribosomal RNA large subunit methyltransferase I OS=Escherichia coli (strain SMS-3-5 / SECEC) OX=439855 GN=rlmI PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 5.2e-43
Identity = 102/302 (33.77%), Postives = 168/302 (55.63%), Query Frame = 0

Query: 27  NAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKAEIEDCVRGIDEINHVKWRPSV 86
           N+YRL+  E D L G+ ID FG+  V+   +A  E  +A +   ++ +     +  R  V
Sbjct: 100 NSYRLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDV 159

Query: 87  EILKEEGIDVSELKDTNPSNCPERTKVTENGIFYAISLE-GQKTGFYADQRENRHFISTI 146
            + K+EG+++++   T     P    + E+G+   + ++ G KTG+Y DQR++R      
Sbjct: 160 AVRKKEGMELTQGPVTGELP-PALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY 219

Query: 147 SEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSLPALELAKENVILNSMDPDRISFLKA 206
            E ++VL+   Y+GGF+++A  GG   V  +D+S  AL++A++NV LN +D  +  F++ 
Sbjct: 220 VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFVRD 279

Query: 207 DATTFMKDALSRNECWDIVILDPPKLAPRKKVLQTASGMYRTLNSLAMQLTRKGGLLMTC 266
           D    ++    R E +D++++DPPK    K  L  A   Y+ +N LA+QL  +GG+L+T 
Sbjct: 280 DVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGVLLTF 339

Query: 267 SCSGAMTQSGMFLQTLQASPGAASMAKKKITVLRQAGAACDHPIDPTYPEGAYLSNILLR 326
           SCSG MT S +F + +     AA  A + +  + Q   A DHP+  TYPEG YL     R
Sbjct: 340 SCSGLMT-SDLFQKII---ADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFACR 396

Query: 327 VL 328
           V+
Sbjct: 400 VM 396

BLAST of Carg03418 vs. ExPASy Swiss-Prot
Match: Q6LQ36 (Ribosomal RNA large subunit methyltransferase I OS=Photobacterium profundum (strain SS9) OX=298386 GN=rlmI PE=3 SV=2)

HSP 1 Score: 176.0 bits (445), Expect = 6.9e-43
Identity = 112/315 (35.56%), Postives = 165/315 (52.38%), Query Frame = 0

Query: 8   RILTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWVEKYKAEI 67
           R+  A+ LR  L      T  YRL+ +E D L G+ ID + +  V    +A  E+ K  +
Sbjct: 83  RLKAAQALRDVLAARDGLT-GYRLIAAESDGLPGITIDRYQNFLVCQLLSAGAEEQKDAL 142

Query: 68  EDCVRGIDEINHVKWRPSVEILKEEGID--VSELKDTNPSNCPERTKVTENGIFYAISL- 127
            + +        V  R  V + K+EG+      L    P   P+   + ENGI   + + 
Sbjct: 143 VEALNICYPECSVYERSDVAVRKKEGLKQRTGVLSGEEP---PKFVTIEENGIKINVDIV 202

Query: 128 EGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSLPALE 187
            G KTGFY DQR++R        G++VL+  CY+GGF L A +GGA  V  +D S PAL+
Sbjct: 203 GGHKTGFYLDQRDSRQAAVKYVNGKRVLNCFCYTGGFGLYALKGGASQVVNVDVSQPALD 262

Query: 188 LAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQTASGM 247
            A+ N   N +  +   F+ AD    +++   R E +D+VI+DPPK A  K  L  A   
Sbjct: 263 TARLNTEANGLPVENAEFVNADVFKLLREYRERGEFFDVVIMDPPKFAESKSQLVGACRG 322

Query: 248 YRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQASPGAASMAKKKITVLRQAGAA 307
           Y+ +N LAMQ+   GG+L+T SCSG M  +G+F + +     AA  A +++  + + G A
Sbjct: 323 YKDINMLAMQILNPGGILLTYSCSGLM-DNGLFQKIV---ADAALDAHREVQFIERFGQA 382

Query: 308 CDHPIDPTYPEGAYL 320
            DHP+D  YPEG YL
Sbjct: 383 ADHPLDSAYPEGFYL 389

BLAST of Carg03418 vs. ExPASy TrEMBL
Match: A0A6J1JEU3 (uncharacterized protein LOC111483780 OS=Cucurbita maxima OX=3661 GN=LOC111483780 PE=4 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 2.4e-176
Identity = 321/327 (98.17%), Postives = 322/327 (98.47%), Query Frame = 0

Query: 1   MQKLIETRILTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV 60
           MQKLIETRI  ARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV
Sbjct: 116 MQKLIETRIHIARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV 175

Query: 61  EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFY 120
           EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFY
Sbjct: 176 EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFY 235

Query: 121 AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL 180
           AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL
Sbjct: 236 AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL 295

Query: 181 PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT 240
           PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT
Sbjct: 296 PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT 355

Query: 241 ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQASPGAASMAKKKITVLRQ 300
           ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQ   GAASMAKK+ITVLRQ
Sbjct: 356 ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQ---GAASMAKKQITVLRQ 415

Query: 301 AGAACDHPIDPTYPEGAYLSNILLRVL 328
           AGAACDHPIDPTYPEGAYLSNILLRVL
Sbjct: 416 AGAACDHPIDPTYPEGAYLSNILLRVL 439

BLAST of Carg03418 vs. ExPASy TrEMBL
Match: A0A6J1FRU8 (uncharacterized protein LOC111448241 OS=Cucurbita moschata OX=3662 GN=LOC111448241 PE=4 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 2.7e-175
Identity = 321/327 (98.17%), Postives = 321/327 (98.17%), Query Frame = 0

Query: 1   MQKLIETRILTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV 60
           MQKLIETRI TARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV
Sbjct: 116 MQKLIETRIHTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV 175

Query: 61  EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFY 120
           EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPS C ERTKVTENGIFY
Sbjct: 176 EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSICLERTKVTENGIFY 235

Query: 121 AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL 180
           AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL
Sbjct: 236 AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL 295

Query: 181 PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT 240
           PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT
Sbjct: 296 PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT 355

Query: 241 ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQASPGAASMAKKKITVLRQ 300
           ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQ   GAASMAKKKITVLRQ
Sbjct: 356 ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQ---GAASMAKKKITVLRQ 415

Query: 301 AGAACDHPIDPTYPEGAYLSNILLRVL 328
           AGAACDHPIDPTYPEGAYLSNILLRVL
Sbjct: 416 AGAACDHPIDPTYPEGAYLSNILLRVL 439

BLAST of Carg03418 vs. ExPASy TrEMBL
Match: A0A0A0KB85 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G135980 PE=4 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 6.0e-167
Identity = 302/327 (92.35%), Postives = 313/327 (95.72%), Query Frame = 0

Query: 1   MQKLIETRILTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV 60
           ++K+IETR+ TARELRK+LGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV
Sbjct: 64  VEKMIETRVHTARELRKNLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV 123

Query: 61  EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFY 120
           EKYKAEIEDCVRGI+EINHVKWRPSV+ILKEEGIDVSELKDT+PS CPERTKV EN IFY
Sbjct: 124 EKYKAEIEDCVRGIEEINHVKWRPSVDILKEEGIDVSELKDTSPSTCPERTKVMENDIFY 183

Query: 121 AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL 180
           AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNA RGGAINVTGIDSSL
Sbjct: 184 AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNALRGGAINVTGIDSSL 243

Query: 181 PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT 240
           PALELAKENV+LNSMDP+RISFLKADAT FMKDALSRNE WD+VILDPPKLAPRKKVLQT
Sbjct: 244 PALELAKENVVLNSMDPERISFLKADATAFMKDALSRNESWDVVILDPPKLAPRKKVLQT 303

Query: 241 ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQASPGAASMAKKKITVLRQ 300
           ASGMYR LNSLAMQLTRKGG LMTCSCSGAMTQSGMFLQ LQ   GAAS AKKKITVLRQ
Sbjct: 304 ASGMYRNLNSLAMQLTRKGGFLMTCSCSGAMTQSGMFLQILQ---GAASTAKKKITVLRQ 363

Query: 301 AGAACDHPIDPTYPEGAYLSNILLRVL 328
           AGAACDHPIDPTYPEGAYLSNILLRVL
Sbjct: 364 AGAACDHPIDPTYPEGAYLSNILLRVL 387

BLAST of Carg03418 vs. ExPASy TrEMBL
Match: A0A5A7UHS0 (Ribosomal RNA large subunit methyltransferase I isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold104G00040 PE=4 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 1.3e-166
Identity = 302/327 (92.35%), Postives = 313/327 (95.72%), Query Frame = 0

Query: 1   MQKLIETRILTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV 60
           ++K+IETRI TARELRK+LGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV
Sbjct: 103 VEKMIETRIHTARELRKNLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV 162

Query: 61  EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFY 120
           EKYKAEIEDCVRGI+EINHVKWRPSV+ILKEEGIDVSELK+T+PS CPERTKV ENGIFY
Sbjct: 163 EKYKAEIEDCVRGIEEINHVKWRPSVDILKEEGIDVSELKETSPSTCPERTKVKENGIFY 222

Query: 121 AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL 180
            ISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNA RGGAINVTGIDSSL
Sbjct: 223 TISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNALRGGAINVTGIDSSL 282

Query: 181 PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT 240
           PALELAKENV+LNSMDP+RISFLKADAT FMKDALSRNE WDIVILDPPKLAPRKKVLQT
Sbjct: 283 PALELAKENVVLNSMDPERISFLKADATAFMKDALSRNESWDIVILDPPKLAPRKKVLQT 342

Query: 241 ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQASPGAASMAKKKITVLRQ 300
           ASGMYR LNSLAMQLTRKGG LMTCSCSGAMTQSGMFLQ LQ   GAASMAKKKITVLR 
Sbjct: 343 ASGMYRNLNSLAMQLTRKGGFLMTCSCSGAMTQSGMFLQILQ---GAASMAKKKITVLRP 402

Query: 301 AGAACDHPIDPTYPEGAYLSNILLRVL 328
           AGAACDHPIDP+YPEGAYLSNILLRVL
Sbjct: 403 AGAACDHPIDPSYPEGAYLSNILLRVL 426

BLAST of Carg03418 vs. ExPASy TrEMBL
Match: A0A1S3C3K0 (ribosomal RNA large subunit methyltransferase I isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496280 PE=4 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 1.3e-166
Identity = 302/327 (92.35%), Postives = 313/327 (95.72%), Query Frame = 0

Query: 1   MQKLIETRILTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV 60
           ++K+IETRI TARELRK+LGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV
Sbjct: 116 VEKMIETRIHTARELRKNLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV 175

Query: 61  EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFY 120
           EKYKAEIEDCVRGI+EINHVKWRPSV+ILKEEGIDVSELK+T+PS CPERTKV ENGIFY
Sbjct: 176 EKYKAEIEDCVRGIEEINHVKWRPSVDILKEEGIDVSELKETSPSTCPERTKVKENGIFY 235

Query: 121 AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL 180
            ISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNA RGGAINVTGIDSSL
Sbjct: 236 TISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNALRGGAINVTGIDSSL 295

Query: 181 PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT 240
           PALELAKENV+LNSMDP+RISFLKADAT FMKDALSRNE WDIVILDPPKLAPRKKVLQT
Sbjct: 296 PALELAKENVVLNSMDPERISFLKADATAFMKDALSRNESWDIVILDPPKLAPRKKVLQT 355

Query: 241 ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQASPGAASMAKKKITVLRQ 300
           ASGMYR LNSLAMQLTRKGG LMTCSCSGAMTQSGMFLQ LQ   GAASMAKKKITVLR 
Sbjct: 356 ASGMYRNLNSLAMQLTRKGGFLMTCSCSGAMTQSGMFLQILQ---GAASMAKKKITVLRP 415

Query: 301 AGAACDHPIDPTYPEGAYLSNILLRVL 328
           AGAACDHPIDP+YPEGAYLSNILLRVL
Sbjct: 416 AGAACDHPIDPSYPEGAYLSNILLRVL 439

BLAST of Carg03418 vs. TAIR 10
Match: AT1G54310.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 494.2 bits (1271), Expect = 8.1e-140
Identity = 242/327 (74.01%), Postives = 284/327 (86.85%), Query Frame = 0

Query: 1   MQKLIETRILTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV 60
           ++KL++TRI  A +LRKSL LPSA+TNAYRLVNSEGDRLSGLI+DVFGD+AVVASSAAW+
Sbjct: 131 IEKLLQTRIAEAVQLRKSLALPSANTNAYRLVNSEGDRLSGLIVDVFGDIAVVASSAAWL 190

Query: 61  EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKVTENGIFY 120
           EKY+ E+E C+R ID INH+ WRPS+++LKE+G D+S LK T  S  P+R+ V ENGI Y
Sbjct: 191 EKYRIEVESCLRSIDGINHINWRPSLDVLKEDGFDISSLKQTQSSTLPQRSMVVENGISY 250

Query: 121 AISLEGQKTGFYADQRENRHFISTISEGQKVLDLCCYSGGFSLNAARGGAINVTGIDSSL 180
           AISLEGQKTGFY DQRENRHFISTIS G++VLDLCCYSGGF+LNAARGGA +V GIDSSL
Sbjct: 251 AISLEGQKTGFYTDQRENRHFISTISAGKRVLDLCCYSGGFALNAARGGATSVIGIDSSL 310

Query: 181 PALELAKENVILNSMDPDRISFLKADATTFMKDALSRNECWDIVILDPPKLAPRKKVLQT 240
           PALELA+ENVILN+MDP+++ F K D+T FMK ALSR E WDIVILDPPKLAPRKKVL  
Sbjct: 311 PALELARENVILNNMDPEKVVFFKQDSTEFMKGALSREETWDIVILDPPKLAPRKKVLHN 370

Query: 241 ASGMYRTLNSLAMQLTRKGGLLMTCSCSGAMTQSGMFLQTLQASPGAASMAKKKITVLRQ 300
           ASGMYR LNSLAM+LTR GGL+MTCSCSGAMTQSG FL  LQ+   AA+M+ +KITV+R+
Sbjct: 371 ASGMYRNLNSLAMRLTRSGGLMMTCSCSGAMTQSGKFLGILQS---AAAMSGRKITVVRE 430

Query: 301 AGAACDHPIDPTYPEGAYLSNILLRVL 328
           AGAA DHP+DP+YP+G YLSN+LLRVL
Sbjct: 431 AGAASDHPLDPSYPQGQYLSNLLLRVL 454

BLAST of Carg03418 vs. TAIR 10
Match: AT1G54310.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 164.1 bits (414), Expect = 1.9e-40
Identity = 76/113 (67.26%), Postives = 96/113 (84.96%), Query Frame = 0

Query: 1   MQKLIETRILTARELRKSLGLPSASTNAYRLVNSEGDRLSGLIIDVFGDLAVVASSAAWV 60
           ++KL++TRI  A +LRKSL LPSA+TNAYRLVNSEGDRLSGLI+DVFGD+AVVASSAAW+
Sbjct: 114 IEKLLQTRIAEAVQLRKSLALPSANTNAYRLVNSEGDRLSGLIVDVFGDIAVVASSAAWL 173

Query: 61  EKYKAEIEDCVRGIDEINHVKWRPSVEILKEEGIDVSELKDTNPSNCPERTKV 114
           EKY+ E+E C+R ID INH+ WRPS+++LKE+G D+S LK T  S  P+R+ V
Sbjct: 174 EKYRIEVESCLRSIDGINHINWRPSLDVLKEDGFDISSLKQTQSSTLPQRSMV 226

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7010450.13.0e-181100.00rlmI, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6570599.15.4e-17899.08hypothetical protein SDJN03_29514, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022985853.15.0e-17698.17uncharacterized protein LOC111483780 [Cucurbita maxima][more]
XP_022943486.15.6e-17598.17uncharacterized protein LOC111448241 [Cucurbita moschata][more]
XP_023512212.12.3e-17397.55uncharacterized protein LOC111777007 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
B4RRY88.7e-4637.07Ribosomal RNA large subunit methyltransferase I OS=Alteromonas mediterranea (str... [more]
Q3IKJ03.6e-4434.86Ribosomal RNA large subunit methyltransferase I OS=Pseudoalteromonas translucida... [more]
B2VDG51.4e-4333.64Ribosomal RNA large subunit methyltransferase I OS=Erwinia tasmaniensis (strain ... [more]
B1LJ305.2e-4333.77Ribosomal RNA large subunit methyltransferase I OS=Escherichia coli (strain SMS-... [more]
Q6LQ366.9e-4335.56Ribosomal RNA large subunit methyltransferase I OS=Photobacterium profundum (str... [more]
Match NameE-valueIdentityDescription
A0A6J1JEU32.4e-17698.17uncharacterized protein LOC111483780 OS=Cucurbita maxima OX=3661 GN=LOC111483780... [more]
A0A6J1FRU82.7e-17598.17uncharacterized protein LOC111448241 OS=Cucurbita moschata OX=3662 GN=LOC1114482... [more]
A0A0A0KB856.0e-16792.35Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G135980 PE=4 SV=1[more]
A0A5A7UHS01.3e-16692.35Ribosomal RNA large subunit methyltransferase I isoform X1 OS=Cucumis melo var. ... [more]
A0A1S3C3K01.3e-16692.35ribosomal RNA large subunit methyltransferase I isoform X1 OS=Cucumis melo OX=36... [more]
Match NameE-valueIdentityDescription
AT1G54310.28.1e-14074.01S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G54310.11.9e-4067.26S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.750.80RNA methyltransferase domain (HRMD) likecoord: 1..104
e-value: 7.8E-21
score: 76.2
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 111..327
e-value: 5.0E-69
score: 233.9
NoneNo IPR availablePANTHERPTHR42873RIBOSOMAL RNA LARGE SUBUNIT METHYLTRANSFERASEcoord: 3..327
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 150..265
e-value: 4.48705E-14
score: 65.529
NoneNo IPR availableCDDcd11572RlmI_M_likecoord: 4..98
e-value: 8.64362E-26
score: 96.7628
IPR019614S-adenosylmethionine-dependent methyltransferasePFAMPF10672Methyltrans_SAMcoord: 108..280
e-value: 2.4E-18
score: 66.3
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 7..322

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg03418-RACarg03418-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0003723 RNA binding