Carg03413 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg03413
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Ferrochelatase
Location	Carg_Chr20: 1469668 .. 1470938 (-)
RNA-Seq Expression	Carg03413
Synteny	Carg03413

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTGCATTTTCACTCACTTGTTCAATGCATGGTTTGTGAGTGCAATGGGGGATGCATGATTGCTTGAAGATATGTTGCCTTCTGTTTATGCGTTTTTTTTTTTTTAGGAAATAGGAACTACTTCATTCATTCAATCAATCAAAAGAAATCATATTGGCCTGATGAAGAAAGGGACAAGGTGTTCCATTCCCAAAAATTAGTAAGACTACATAAAAGCCCTGCAATCTAAGTTATGATGATTGCTCCTATTGGAATCTATGACTTGTACAAGAATTCTAGTTGACAACAGTAATCTGAACATGAGCGTAGCTTAACCGACAAAGTATCTACCTTCGACTAAGAGGTTAAATATTCGAATCTCTCCCCCTGCATAGTAGTCTAAAGAAGTGTACTCATCTAAAAACACTTCCGCATTTCATTAAGGGGTTCGAGGGCTCCATAGACCTCAAGGCAGGCTCTATAGTGTACTTTGTTCGAGGGAAGGATTGTTGGGAGAGGGGTCCCACATCGGCTAATAGTGTACATGTTGTTGGTACGAGGCCTTTTGGGAATACCAAAAGCAAAACCATACTACTGTGGAGATTCATGGTTCCTAACGGGAACAACAATGTCTTATGGTAAGCTTACAAACTCTAAATTTTGGTACACGAACAAAATCATTGTGTATGTTTATTGATGCATCTATCAAATCTTTTCTTTTTCCTCGTAGATAAAAAAAGACAGGATATCAAAGCTTGTTGTTATTCCTCTTTATCCCCAATTTTCAATATGCACAAGTGGTTCGAACCTTGGGCTGTTGGAGGGTATCTTCCGGTAAAGGCTAGATCCTTTATGTTCATACAATATTCTGACATCCTGGATTGGTTTAAATGACAATAGAACTTTCTTTTTGGTTCATAAGTGTTACTATTCATGCCTTGGCATCAAAATGTAACTTGAACAAATACATAACCATCACTTATGAGTTGATGATACTATTTGCATATTGACTGCGTGCAGATTACCTAGTTTAGAAATCAAAATCATATTCAGTCAATTAAATTTTTATGCTATACTTCATTTGTAGCTTTGTAAGTGAGAACATAGAAACTTTGGAAGAAATAGACGTGGAATATAAAGAACTGGCTTTAAAATCTAGGATAAGAAAATGGGGACGTGTTCCAGCGTTAGGATGTGAACCTACTTTCATAACAGATTTGGCTGATGCTGTAATTGAGAGTCTTCCATATGTCAGAGCAATGGCTGTCTCAAATCTTGAAGCTCGCCAG

mRNA sequence

ATGTCTGCATTTTCACTCACTTGTTCAATGCATGGAAATAGGAACTACTTCATTCATTCAATCAATCAAAAGAAATCATATTGGCCTGATGAAGAAAGGGACAAGATAAAAAAAGACAGGATATCAAAGCTTGTTGTTATTCCTCTTTATCCCCAATTTTCAATATGCACAAGTGGTTCGAACCTTGGGCTGTTGGAGGGTATCTTCCGCTTTGTAAGTGAGAACATAGAAACTTTGGAAGAAATAGACGTGGAATATAAAGAACTGGCTTTAAAATCTAGGATAAGAAAATGGGGACGTGTTCCAGCGTTAGGATGTGAACCTACTTTCATAACAGATTTGGCTGATGCTGTAATTGAGAGTCTTCCATATGTCAGAGCAATGGCTGTCTCAAATCTTGAAGCTCGCCAG

Coding sequence (CDS)

ATGTCTGCATTTTCACTCACTTGTTCAATGCATGGAAATAGGAACTACTTCATTCATTCAATCAATCAAAAGAAATCATATTGGCCTGATGAAGAAAGGGACAAGATAAAAAAAGACAGGATATCAAAGCTTGTTGTTATTCCTCTTTATCCCCAATTTTCAATATGCACAAGTGGTTCGAACCTTGGGCTGTTGGAGGGTATCTTCCGCTTTGTAAGTGAGAACATAGAAACTTTGGAAGAAATAGACGTGGAATATAAAGAACTGGCTTTAAAATCTAGGATAAGAAAATGGGGACGTGTTCCAGCGTTAGGATGTGAACCTACTTTCATAACAGATTTGGCTGATGCTGTAATTGAGAGTCTTCCATATGTCAGAGCAATGGCTGTCTCAAATCTTGAAGCTCGCCAG

Protein sequence

MSAFSLTCSMHGNRNYFIHSINQKKSYWPDEERDKIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFRFVSENIETLEEIDVEYKELALKSRIRKWGRVPALGCEPTFITDLADAVIESLPYVRAMAVSNLEARQ

Homology

[Close]

BLAST of Carg03413 vs. NCBI nr
Match: KAG7010455.1 (Ferrochelatase-2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 277.7 bits (709), Expect = 5.3e-71
Identity = 137/137 (100.00%), Postives = 137/137 (100.00%), Query Frame = 0


Query: 1   MSAFSLTCSMHGNRNYFIHSINQKKSYWPDEERDKIKKDRISKLVVIPLYPQFSICTSGS 60
           MSAFSLTCSMHGNRNYFIHSINQKKSYWPDEERDKIKKDRISKLVVIPLYPQFSICTSGS
Sbjct: 1   MSAFSLTCSMHGNRNYFIHSINQKKSYWPDEERDKIKKDRISKLVVIPLYPQFSICTSGS 60

Query: 61  NLGLLEGIFRFVSENIETLEEIDVEYKELALKSRIRKWGRVPALGCEPTFITDLADAVIE 120
           NLGLLEGIFRFVSENIETLEEIDVEYKELALKSRIRKWGRVPALGCEPTFITDLADAVIE
Sbjct: 61  NLGLLEGIFRFVSENIETLEEIDVEYKELALKSRIRKWGRVPALGCEPTFITDLADAVIE 120

Query: 121 SLPYVRAMAVSNLEARQ 138
           SLPYVRAMAVSNLEARQ
Sbjct: 121 SLPYVRAMAVSNLEARQ 137

[Close]

BLAST of Carg03413 vs. NCBI nr
Match: EOX94122.1 (Ferrochelatase 2 isoform 3 [Theobroma cacao])

HSP 1 Score: 151.8 bits (382), Expect = 4.4e-33
Identity = 89/158 (56.33%), Postives = 98/158 (62.03%), Query Frame = 0


Query: 27  YW---PDEERDKIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR------------- 86
           YW    +E  ++IKKD I+KLVV+PLYPQFSI TSGS+L LLE IFR             
Sbjct: 203 YWHPFTEEAIEQIKKDGITKLVVLPLYPQFSISTSGSSLRLLESIFRDDEYLVNMRHTVI 262

Query: 87  -------------------------------FVSENIETLEEIDVEYKELALKSRIRKWG 138
                                          FVSE+IETLEEIDVEYKELA +S I  WG
Sbjct: 263 PSWYQREGYIKSMANLIEKELQKFDLPEKVSFVSEHIETLEEIDVEYKELAFESGIENWG 322

[Close]

BLAST of Carg03413 vs. NCBI nr
Match: XP_011011647.1 (PREDICTED: ferrochelatase-2, chloroplastic-like isoform X3 [Populus euphratica])

HSP 1 Score: 146.0 bits (367), Expect = 2.4e-31
Identity = 92/190 (48.42%), Postives = 101/190 (53.16%), Query Frame = 0


Query: 27  YW---PDEERDKIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR------------- 86
           YW    +E   +IK+DRI+KLVV+PLYPQFSI TSGS+L LLE IFR             
Sbjct: 255 YWHPFTEEAIQQIKRDRITKLVVLPLYPQFSISTSGSSLRLLERIFREDEYLVSMQHTVI 314

Query: 87  ------------------------------------------------------------ 138
                                                                       
Sbjct: 315 PSWYQREGYIKALANLIGKELETFDHPEQSRVGPVEWLKPYTDETIIELGKRGVKCLLAV 374

[Close]

BLAST of Carg03413 vs. NCBI nr
Match: XP_011011650.1 (PREDICTED: ferrochelatase-2, chloroplastic-like isoform X5 [Populus euphratica])

HSP 1 Score: 146.0 bits (367), Expect = 2.4e-31
Identity = 92/190 (48.42%), Postives = 101/190 (53.16%), Query Frame = 0


Query: 27  YW---PDEERDKIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR------------- 86
           YW    +E   +IK+DRI+KLVV+PLYPQFSI TSGS+L LLE IFR             
Sbjct: 206 YWHPFTEEAIQQIKRDRITKLVVLPLYPQFSISTSGSSLRLLERIFREDEYLVSMQHTVI 265

Query: 87  ------------------------------------------------------------ 138
                                                                       
Sbjct: 266 PSWYQREGYIKALANLIGKELETFDHPEQSRVGPVEWLKPYTDETIIELGKRGVKCLLAV 325

[Close]

BLAST of Carg03413 vs. NCBI nr
Match: EOX94121.1 (Ferrochelatase 2 isoform 2 [Theobroma cacao])

HSP 1 Score: 139.4 bits (350), Expect = 2.3e-29
Identity = 89/190 (46.84%), Postives = 98/190 (51.58%), Query Frame = 0


Query: 27  YW---PDEERDKIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR------------- 86
           YW    +E  ++IKKD I+KLVV+PLYPQFSI TSGS+L LLE IFR             
Sbjct: 203 YWHPFTEEAIEQIKKDGITKLVVLPLYPQFSISTSGSSLRLLESIFRDDEYLVNMRHTVI 262

Query: 87  ------------------------------------------------------------ 138
                                                                       
Sbjct: 263 PSWYQREGYIKSMANLIEKELQKFDLPEKSRVGPVEWLKPYTDETIVELGRKGVKSLLAV 322

[Close]

BLAST of Carg03413 vs. ExPASy Swiss-Prot
Match: Q0DIV0 (Ferrochelatase-2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0361200 PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 2.0e-28
Identity = 92/241 (38.17%), Postives = 102/241 (42.32%), Query Frame = 0


Query: 27  YW---PDEERDKIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR------------- 86
           YW    +E  ++IK+D I+KLVV+PLYPQFSI TSGS+L LLEGIFR             
Sbjct: 212 YWHPFTEEAIEQIKRDGITKLVVLPLYPQFSISTSGSSLRLLEGIFREDEYLVNMQHTVI 271

Query: 87  ------------------------------------------------------------ 138
                                                                       
Sbjct: 272 PSWYQREGYIKAMATLIEKELRTFSEPQKVMIFFSAHGVPLAYVEEAGDPYKAEMEECVD 331

[Close]

BLAST of Carg03413 vs. ExPASy Swiss-Prot
Match: A2Y3Q5 (Ferrochelatase-2, chloroplastic OS=Oryza sativa subsp. indica OX=39946 GN=HEMH PE=2 SV=2)

HSP 1 Score: 126.7 bits (317), Expect = 2.0e-28
Identity = 92/241 (38.17%), Postives = 102/241 (42.32%), Query Frame = 0


Query: 27  YW---PDEERDKIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR------------- 86
           YW    +E  ++IK+D I+KLVV+PLYPQFSI TSGS+L LLEGIFR             
Sbjct: 212 YWHPFTEEAIEQIKRDGITKLVVLPLYPQFSISTSGSSLRLLEGIFREDEYLVNMQHTVI 271

Query: 87  ------------------------------------------------------------ 138
                                                                       
Sbjct: 272 PSWYQREGYIKAMATLIEKELRTFSEPQKVMIFFSAHGVPLAYVEEAGDPYKAEMEECVD 331

[Close]

BLAST of Carg03413 vs. ExPASy Swiss-Prot
Match: O04921 (Ferrochelatase-2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FC2 PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.2e-25
Identity = 88/241 (36.51%), Postives = 99/241 (41.08%), Query Frame = 0


Query: 27  YW---PDEERDKIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR------------- 86
           YW    +E  ++IK+D I+KLVV+PLYPQFSI TSGS+L LLE IFR             
Sbjct: 199 YWHPFTEEAIEQIKRDGITKLVVLPLYPQFSISTSGSSLRLLERIFREDEYLVNMQHTVI 258

Query: 87  ------------------------------------------------------------ 138
                                                                       
Sbjct: 259 PSWYQREGYIKAMANLIQSELGKFGSPNQVVIFFSAHGVPLAYVEEAGDPYKAEMEECVD 318

[Close]

BLAST of Carg03413 vs. ExPASy Swiss-Prot
Match: P42043 (Ferrochelatase-1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FC1 PE=2 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 4.2e-18
Identity = 45/62 (72.58%), Postives = 52/62 (83.87%), Query Frame = 0


Query: 71  FVSENIETLEEIDVEYKELALKSRIRKWGRVPALGCEPTFITDLADAVIESLPYVRAMAV 130
           FVSE+IETLEEID+EY+ELAL+S +  WGRVPALG  P+FITDLADAVIESLP   AM+ 
Sbjct: 362 FVSEHIETLEEIDMEYRELALESGVENWGRVPALGLTPSFITDLADAVIESLPSAEAMSN 421

Query: 131 SN 133
            N
Sbjct: 422 PN 423

HSP 2 Score: 53.9 bits (128), Expect = 1.6e-06
Identity = 24/47 (51.06%), Postives = 37/47 (78.72%), Query Frame = 0


Query: 27  YW---PDEERDKIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR 71
           YW    +E   +IKKD+I++LVV+PLYPQ+SI T+GS++ +L+ +FR
Sbjct: 188 YWYPFTEEAVQQIKKDKITRLVVLPLYPQYSISTTGSSIRVLQDLFR 234

[Close]

BLAST of Carg03413 vs. ExPASy Swiss-Prot
Match: P42045 (Ferrochelatase-2, chloroplastic OS=Hordeum vulgare OX=4513 GN=HEMH PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.2e-17
Identity = 43/64 (67.19%), Postives = 53/64 (82.81%), Query Frame = 0


Query: 71  FVSENIETLEEIDVEYKELALKSRIRKWGRVPALGCEPTFITDLADAVIESLPYVRAMAV 130
           FVSE+IETLEEID+EY+ELAL+S I  WGRVPALGC  +FI+DLADAV+E+LP   AMA 
Sbjct: 381 FVSEHIETLEEIDMEYRELALESGIENWGRVPALGCTSSFISDLADAVVEALPSASAMAT 440

Query: 131 SNLE 135
             ++
Sbjct: 441 RKVK 444

HSP 2 Score: 55.8 bits (133), Expect = 4.3e-07
Identity = 26/47 (55.32%), Postives = 37/47 (78.72%), Query Frame = 0


Query: 27  YW---PDEERDKIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR 71
           YW    +E  D+IKKD+I+KLVV+PLYPQ+SI TSGS++ +L+ I +
Sbjct: 207 YWYPFTEEAIDQIKKDKITKLVVLPLYPQYSISTSGSSIRVLQNIVK 253

[Close]

BLAST of Carg03413 vs. ExPASy TrEMBL
Match: A0A061DNX9 (Ferrochelatase 2 isoform 3 OS=Theobroma cacao OX=3641 GN=TCM_003334 PE=3 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 2.1e-33
Identity = 89/158 (56.33%), Postives = 98/158 (62.03%), Query Frame = 0


Query: 27  YW---PDEERDKIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR------------- 86
           YW    +E  ++IKKD I+KLVV+PLYPQFSI TSGS+L LLE IFR             
Sbjct: 203 YWHPFTEEAIEQIKKDGITKLVVLPLYPQFSISTSGSSLRLLESIFRDDEYLVNMRHTVI 262

Query: 87  -------------------------------FVSENIETLEEIDVEYKELALKSRIRKWG 138
                                          FVSE+IETLEEIDVEYKELA +S I  WG
Sbjct: 263 PSWYQREGYIKSMANLIEKELQKFDLPEKVSFVSEHIETLEEIDVEYKELAFESGIENWG 322

[Close]

BLAST of Carg03413 vs. ExPASy TrEMBL
Match: A0A061DPW8 (Ferrochelatase 2 isoform 2 OS=Theobroma cacao OX=3641 GN=TCM_003334 PE=3 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 1.1e-29
Identity = 89/190 (46.84%), Postives = 98/190 (51.58%), Query Frame = 0


Query: 27  YW---PDEERDKIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR------------- 86
           YW    +E  ++IKKD I+KLVV+PLYPQFSI TSGS+L LLE IFR             
Sbjct: 203 YWHPFTEEAIEQIKKDGITKLVVLPLYPQFSISTSGSSLRLLESIFRDDEYLVNMRHTVI 262

Query: 87  ------------------------------------------------------------ 138
                                                                       
Sbjct: 263 PSWYQREGYIKSMANLIEKELQKFDLPEKSRVGPVEWLKPYTDETIVELGRKGVKSLLAV 322

[Close]

BLAST of Carg03413 vs. ExPASy TrEMBL
Match: A0A6P6FXM4 (ferrochelatase-2, chloroplastic isoform X2 OS=Ziziphus jujuba OX=326968 GN=LOC107412374 PE=3 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 2.5e-29
Identity = 88/190 (46.32%), Postives = 97/190 (51.05%), Query Frame = 0


Query: 27  YW---PDEERDKIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR------------- 86
           YW    +E   +IK+D I+KLVV+PLYPQFSI TSGS+L LLE IFR             
Sbjct: 200 YWHPFTEEAIQQIKRDGITKLVVLPLYPQFSISTSGSSLRLLESIFREDEYLVNMQHTVI 259

Query: 87  ------------------------------------------------------------ 138
                                                                       
Sbjct: 260 PSWYQREGYIKAMANLIEMELKIFDSPDKSRVGPVEWLKPYTDETIIDLGKKGVKSLLAV 319

[Close]

BLAST of Carg03413 vs. ExPASy TrEMBL
Match: A0A368PN35 (Ferrochelatase OS=Setaria italica OX=4555 GN=SETIT_1G195800v2 PE=3 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 2.5e-29
Identity = 90/202 (44.55%), Postives = 101/202 (50.00%), Query Frame = 0


Query: 27  YW---PDEERDKIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR------------- 86
           YW    +E  ++IK+D I+KLVV+PLYPQFSI TSGS+L LLE IFR             
Sbjct: 201 YWHPFTEEAIEQIKRDGITKLVVLPLYPQFSISTSGSSLRLLESIFREDEYLVNMQHTVI 260

Query: 87  ------------------------------------------------------------ 138
                                                                       
Sbjct: 261 PSWYQREGYIKAMATLIENELTKFSEPQKVMIFFSAHGVPLAYVEEAGDPYKAEMEECID 320

[Close]

BLAST of Carg03413 vs. ExPASy TrEMBL
Match: A0A1S4E153 (Ferrochelatase OS=Cucumis melo OX=3656 GN=LOC103496275 PE=3 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 3.5e-28
Identity = 96/241 (39.83%), Postives = 103/241 (42.74%), Query Frame = 0


Query: 27  YW---PDEERDKIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR------------- 86
           YW    +E  ++IKKDRISKLVV+PLYPQFSI TSGS+L LLEGIFR             
Sbjct: 208 YWHPFTEEAIEQIKKDRISKLVVLPLYPQFSISTSGSSLRLLEGIFREDEYLVNMQHTVI 267

Query: 87  ------------------------------------------------------------ 138
                                                                       
Sbjct: 268 PSWYQREGYIKAMADLIEKELRTFDFPEQVMVFFSAHGVPLAYVEEAGDPYKAEMEECVD 327

[Close]

BLAST of Carg03413 vs. TAIR 10
Match: AT2G30390.1 (ferrochelatase 2 )

HSP 1 Score: 117.5 bits (293), Expect = 8.6e-27
Identity = 88/241 (36.51%), Postives = 99/241 (41.08%), Query Frame = 0


Query: 27  YW---PDEERDKIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR------------- 86
           YW    +E  ++IK+D I+KLVV+PLYPQFSI TSGS+L LLE IFR             
Sbjct: 199 YWHPFTEEAIEQIKRDGITKLVVLPLYPQFSISTSGSSLRLLERIFREDEYLVNMQHTVI 258

Query: 87  ------------------------------------------------------------ 138
                                                                       
Sbjct: 259 PSWYQREGYIKAMANLIQSELGKFGSPNQVVIFFSAHGVPLAYVEEAGDPYKAEMEECVD 318

[Close]

BLAST of Carg03413 vs. TAIR 10
Match: AT2G30390.2 (ferrochelatase 2 )

HSP 1 Score: 115.9 bits (289), Expect = 2.5e-26
Identity = 58/67 (86.57%), Postives = 62/67 (92.54%), Query Frame = 0


Query: 71  FVSENIETLEEIDVEYKELALKSRIRKWGRVPALGCEPTFITDLADAVIESLPYVRAMAV 130
           FVSE+IETLEEIDVEYKELALKS I+ WGRVPALG EP FI+DLADAV+ESLPYV AMAV
Sbjct: 383 FVSEHIETLEEIDVEYKELALKSGIKNWGRVPALGTEPMFISDLADAVVESLPYVGAMAV 442

Query: 131 SNLEARQ 138
           SNLEARQ
Sbjct: 443 SNLEARQ 449

HSP 2 Score: 58.9 bits (141), Expect = 3.6e-09
Identity = 30/47 (63.83%), Postives = 37/47 (78.72%), Query Frame = 0


Query: 27  YW---PDEERDKIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR 71
           YW    +E  ++IK+D I+KLVV+PLYPQFSI TSGS+L LLE IFR
Sbjct: 199 YWHPFTEEAIEQIKRDGITKLVVLPLYPQFSISTSGSSLRLLERIFR 245

[Close]

BLAST of Carg03413 vs. TAIR 10
Match: AT5G26030.1 (ferrochelatase 1 )

HSP 1 Score: 92.4 bits (228), Expect = 3.0e-19
Identity = 45/62 (72.58%), Postives = 52/62 (83.87%), Query Frame = 0


Query: 71  FVSENIETLEEIDVEYKELALKSRIRKWGRVPALGCEPTFITDLADAVIESLPYVRAMAV 130
           FVSE+IETLEEID+EY+ELAL+S +  WGRVPALG  P+FITDLADAVIESLP   AM+ 
Sbjct: 362 FVSEHIETLEEIDMEYRELALESGVENWGRVPALGLTPSFITDLADAVIESLPSAEAMSN 421

Query: 131 SN 133
            N
Sbjct: 422 PN 423

HSP 2 Score: 53.9 bits (128), Expect = 1.2e-07
Identity = 24/47 (51.06%), Postives = 37/47 (78.72%), Query Frame = 0


Query: 27  YW---PDEERDKIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR 71
           YW    +E   +IKKD+I++LVV+PLYPQ+SI T+GS++ +L+ +FR
Sbjct: 188 YWYPFTEEAVQQIKKDKITRLVVLPLYPQYSISTTGSSIRVLQDLFR 234

[Close]

BLAST of Carg03413 vs. TAIR 10
Match: AT5G26030.2 (ferrochelatase 1 )

HSP 1 Score: 92.4 bits (228), Expect = 3.0e-19
Identity = 45/62 (72.58%), Postives = 52/62 (83.87%), Query Frame = 0


Query: 71  FVSENIETLEEIDVEYKELALKSRIRKWGRVPALGCEPTFITDLADAVIESLPYVRAMAV 130
           FVSE+IETLEEID+EY+ELAL+S +  WGRVPALG  P+FITDLADAVIESLP   AM+ 
Sbjct: 362 FVSEHIETLEEIDMEYRELALESGVENWGRVPALGLTPSFITDLADAVIESLPSAEAMSN 421

Query: 131 SN 133
            N
Sbjct: 422 PN 423

HSP 2 Score: 53.9 bits (128), Expect = 1.2e-07
Identity = 24/47 (51.06%), Postives = 37/47 (78.72%), Query Frame = 0


Query: 27  YW---PDEERDKIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR 71
           YW    +E   +IKKD+I++LVV+PLYPQ+SI T+GS++ +L+ +FR
Sbjct: 188 YWYPFTEEAVQQIKKDKITRLVVLPLYPQYSISTTGSSIRVLQDLFR 234

The following BLAST results are available for this feature:

BLAST of Carg03413 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7010455.1	5.3e-71	100.00	Ferrochelatase-2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosp...	[more]
EOX94122.1	4.4e-33	56.33	Ferrochelatase 2 isoform 3 [Theobroma cacao]	[more]
XP_011011647.1	2.4e-31	48.42	PREDICTED: ferrochelatase-2, chloroplastic-like isoform X3 [Populus euphratica]	[more]
XP_011011650.1	2.4e-31	48.42	PREDICTED: ferrochelatase-2, chloroplastic-like isoform X5 [Populus euphratica]	[more]
EOX94121.1	2.3e-29	46.84	Ferrochelatase 2 isoform 2 [Theobroma cacao]	[more]

BLAST of Carg03413 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0DIV0	2.0e-28	38.17	Ferrochelatase-2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05...	[more]
A2Y3Q5	2.0e-28	38.17	Ferrochelatase-2, chloroplastic OS=Oryza sativa subsp. indica OX=39946 GN=HEMH P...	[more]
O04921	1.2e-25	36.51	Ferrochelatase-2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FC2 PE=1 SV=1	[more]
P42043	4.2e-18	72.58	Ferrochelatase-1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN...	[more]
P42045	1.2e-17	67.19	Ferrochelatase-2, chloroplastic OS=Hordeum vulgare OX=4513 GN=HEMH PE=2 SV=1	[more]

BLAST of Carg03413 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A061DNX9	2.1e-33	56.33	Ferrochelatase 2 isoform 3 OS=Theobroma cacao OX=3641 GN=TCM_003334 PE=3 SV=1	[more]
A0A061DPW8	1.1e-29	46.84	Ferrochelatase 2 isoform 2 OS=Theobroma cacao OX=3641 GN=TCM_003334 PE=3 SV=1	[more]
A0A6P6FXM4	2.5e-29	46.32	ferrochelatase-2, chloroplastic isoform X2 OS=Ziziphus jujuba OX=326968 GN=LOC10...	[more]
A0A368PN35	2.5e-29	44.55	Ferrochelatase OS=Setaria italica OX=4555 GN=SETIT_1G195800v2 PE=3 SV=1	[more]
A0A1S4E153	3.5e-28	39.83	Ferrochelatase OS=Cucumis melo OX=3656 GN=LOC103496275 PE=3 SV=1	[more]

BLAST of Carg03413 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G30390.1	8.6e-27	36.51	ferrochelatase 2	[more]
AT2G30390.2	2.5e-26	86.57	ferrochelatase 2	[more]
AT5G26030.1	3.0e-19	72.58	ferrochelatase 1	[more]
AT5G26030.2	3.0e-19	72.58	ferrochelatase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.1400		coord: 29..134 e-value: 1.2E-7 score: 33.4
None	No IPR available	PANTHER	PTHR11108:SF6	FERROCHELATASE	coord: 29..68
None	No IPR available	PANTHER	PTHR11108:SF6	FERROCHELATASE	coord: 69..131
None	No IPR available	SUPERFAMILY	53800	Chelatase	coord: 71..128
IPR001015	Ferrochelatase	PFAM	PF00762	Ferrochelatase	coord: 71..121 e-value: 5.8E-11 score: 42.0
IPR001015	Ferrochelatase	PANTHER	PTHR11108	FERROCHELATASE	coord: 69..131
IPR001015	Ferrochelatase	PANTHER	PTHR11108	FERROCHELATASE	coord: 29..68

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg03413-RA	Carg03413-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006783	heme biosynthetic process
cellular_component	GO:0005622	intracellular anatomical structure
molecular_function	GO:0004325	ferrochelatase activity