Carg03276 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg03276
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	glutamic acid-rich protein-like
Location	Carg_Chr20: 2176367 .. 2177428 (-)
RNA-Seq Expression	Carg03276
Synteny	Carg03276

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGACCGTCACCGGAAGTATCGTTTCTTCGAAGCCAATCTCTATCTCAAAAGCGGCGTCCACTCTCTCCTCCTTTCTCTCCGTCGACAATGGCGCTTCGAAAGCAATTTGCGCCTATCTGAGACGCGCCTCCGCCTCTTTCAACGAGTTAAAGCAGCTCCACAAGGAGTTGAAATCTTCGCGATCTGATCGGAAGCACCGGCATCACGGATCCGAGGCTTCAAACGATCCAGAGGCTTCCAGAGATAATCCACATTGGATTGAAGACGGCGAGAAGAAAAATCCTCTGTATCTAAGAGCTAAAGTTGGAAGAAGCGGTAAGCCGAGTTTTAATGTTCAATCTGAGGATGGGAAGGATGGGAAGACGGAAAAGGAAAGCGGTGGGAATGGTGATTTTGAGGATGCATCGGGTGAATATCGGAAGAGAAAGGTCGAGGACTTGAAGACTGAAATTGAAGATAAACCTAACCGAAAAGTAGAGATGGATGTGGAATCAAGTGATAAAGATAAGAGCGTTGTAGCAGTTGAGACAAAAAGAAAAAAGCACAAGAAAAAGAGCGAGGATAGACACGCTAAGATCGAAGATGACGAACGCGAGAATGGAGCCAGGCGAAGTTACAGTAAATCGCGAATTAGCGATAATAATGGCGAAATTGAAGCTTCTGGGAAGTTCGTCGAGAACAATATCGCAAGCGGAAAAGATAGAAAGAAGCACGAGGACAAGAAGAGTTTGGTTGACGATAAGGATCAAGTAAAGAGTGAAGGTCAGAGAAGAAGAGACGCCGAGGAGGAAAAGAGCACAAACAAGGATAATGATGATGGAGCAGAATCGACCAAGAAGAAAAAGAAGAAGAAGAAGAAGAAGAACAGGGAAGAAGAAGATGACGATTTTCAGAACAACAGTGGAGGAGCTATGGTGAAAGAGGAAATTCCAGTTTCGGATGACAAAGAGTTGAAAAGGAAAGAGAAGAAAAAAAGGAAGAATCGAGGTTTAGAAGAAGGGGGTGATGATGGGTCCGAGGAACAACAGCGTACGAAGAGAAGAAAAGGAAATTTATGA

mRNA sequence

ATGAAGACCGTCACCGGAAGTATCGTTTCTTCGAAGCCAATCTCTATCTCAAAAGCGGCGTCCACTCTCTCCTCCTTTCTCTCCGTCGACAATGGCGCTTCGAAAGCAATTTGCGCCTATCTGAGACGCGCCTCCGCCTCTTTCAACGAGTTAAAGCAGCTCCACAAGGAGTTGAAATCTTCGCGATCTGATCGGAAGCACCGGCATCACGGATCCGAGGCTTCAAACGATCCAGAGGCTTCCAGAGATAATCCACATTGGATTGAAGACGGCGAGAAGAAAAATCCTCTGTATCTAAGAGCTAAAGTTGGAAGAAGCGGTAAGCCGAGTTTTAATGTTCAATCTGAGGATGGGAAGGATGGGAAGACGGAAAAGGAAAGCGGTGGGAATGGTGATTTTGAGGATGCATCGGGTGAATATCGGAAGAGAAAGGTCGAGGACTTGAAGACTGAAATTGAAGATAAACCTAACCGAAAAGTAGAGATGGATGTGGAATCAAGTGATAAAGATAAGAGCGTTGTAGCAGTTGAGACAAAAAGAAAAAAGCACAAGAAAAAGAGCGAGGATAGACACGCTAAGATCGAAGATGACGAACGCGAGAATGGAGCCAGGCGAAGTTACAGTAAATCGCGAATTAGCGATAATAATGGCGAAATTGAAGCTTCTGGGAAGTTCGTCGAGAACAATATCGCAAGCGGAAAAGATAGAAAGAAGCACGAGGACAAGAAGAGTTTGGTTGACGATAAGGATCAAGTAAAGAGTGAAGGTCAGAGAAGAAGAGACGCCGAGGAGGAAAAGAGCACAAACAAGGATAATGATGATGGAGCAGAATCGACCAAGAAGAAAAAGAAGAAGAAGAAGAAGAAGAACAGGGAAGAAGAAGATGACGATTTTCAGAACAACAGTGGAGGAGCTATGGTGAAAGAGGAAATTCCAGTTTCGGATGACAAAGAGTTGAAAAGGAAAGAGAAGAAAAAAAGGAAGAATCGAGGTTTAGAAGAAGGGGGTGATGATGGGTCCGAGGAACAACAGCGTACGAAGAGAAGAAAAGGAAATTTATGA

Coding sequence (CDS)

ATGAAGACCGTCACCGGAAGTATCGTTTCTTCGAAGCCAATCTCTATCTCAAAAGCGGCGTCCACTCTCTCCTCCTTTCTCTCCGTCGACAATGGCGCTTCGAAAGCAATTTGCGCCTATCTGAGACGCGCCTCCGCCTCTTTCAACGAGTTAAAGCAGCTCCACAAGGAGTTGAAATCTTCGCGATCTGATCGGAAGCACCGGCATCACGGATCCGAGGCTTCAAACGATCCAGAGGCTTCCAGAGATAATCCACATTGGATTGAAGACGGCGAGAAGAAAAATCCTCTGTATCTAAGAGCTAAAGTTGGAAGAAGCGGTAAGCCGAGTTTTAATGTTCAATCTGAGGATGGGAAGGATGGGAAGACGGAAAAGGAAAGCGGTGGGAATGGTGATTTTGAGGATGCATCGGGTGAATATCGGAAGAGAAAGGTCGAGGACTTGAAGACTGAAATTGAAGATAAACCTAACCGAAAAGTAGAGATGGATGTGGAATCAAGTGATAAAGATAAGAGCGTTGTAGCAGTTGAGACAAAAAGAAAAAAGCACAAGAAAAAGAGCGAGGATAGACACGCTAAGATCGAAGATGACGAACGCGAGAATGGAGCCAGGCGAAGTTACAGTAAATCGCGAATTAGCGATAATAATGGCGAAATTGAAGCTTCTGGGAAGTTCGTCGAGAACAATATCGCAAGCGGAAAAGATAGAAAGAAGCACGAGGACAAGAAGAGTTTGGTTGACGATAAGGATCAAGTAAAGAGTGAAGGTCAGAGAAGAAGAGACGCCGAGGAGGAAAAGAGCACAAACAAGGATAATGATGATGGAGCAGAATCGACCAAGAAGAAAAAGAAGAAGAAGAAGAAGAAGAACAGGGAAGAAGAAGATGACGATTTTCAGAACAACAGTGGAGGAGCTATGGTGAAAGAGGAAATTCCAGTTTCGGATGACAAAGAGTTGAAAAGGAAAGAGAAGAAAAAAAGGAAGAATCGAGGTTTAGAAGAAGGGGGTGATGATGGGTCCGAGGAACAACAGCGTACGAAGAGAAGAAAAGGAAATTTATGA

Protein sequence

MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKSSRSDRKHRHHGSEASNDPEASRDNPHWIEDGEKKNPLYLRAKVGRSGKPSFNVQSEDGKDGKTEKESGGNGDFEDASGEYRKRKVEDLKTEIEDKPNRKVEMDVESSDKDKSVVAVETKRKKHKKKSEDRHAKIEDDERENGARRSYSKSRISDNNGEIEASGKFVENNIASGKDRKKHEDKKSLVDDKDQVKSEGQRRRDAEEEKSTNKDNDDGAESTKKKKKKKKKKNREEEDDDFQNNSGGAMVKEEIPVSDDKELKRKEKKKRKNRGLEEGGDDGSEEQQRTKRRKGNL

Homology

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BLAST of Carg03276 vs. NCBI nr
Match: KAG7010591.1 (hypothetical protein SDJN02_27385, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 627.1 bits (1616), Expect = 9.3e-176
Identity = 353/353 (100.00%), Postives = 353/353 (100.00%), Query Frame = 0


Query: 1   MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS 60
           MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS
Sbjct: 1   MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS 60

Query: 61  SRSDRKHRHHGSEASNDPEASRDNPHWIEDGEKKNPLYLRAKVGRSGKPSFNVQSEDGKD 120
           SRSDRKHRHHGSEASNDPEASRDNPHWIEDGEKKNPLYLRAKVGRSGKPSFNVQSEDGKD
Sbjct: 61  SRSDRKHRHHGSEASNDPEASRDNPHWIEDGEKKNPLYLRAKVGRSGKPSFNVQSEDGKD 120

Query: 121 GKTEKESGGNGDFEDASGEYRKRKVEDLKTEIEDKPNRKVEMDVESSDKDKSVVAVETKR 180
           GKTEKESGGNGDFEDASGEYRKRKVEDLKTEIEDKPNRKVEMDVESSDKDKSVVAVETKR
Sbjct: 121 GKTEKESGGNGDFEDASGEYRKRKVEDLKTEIEDKPNRKVEMDVESSDKDKSVVAVETKR 180

Query: 181 KKHKKKSEDRHAKIEDDERENGARRSYSKSRISDNNGEIEASGKFVENNIASGKDRKKHE 240
           KKHKKKSEDRHAKIEDDERENGARRSYSKSRISDNNGEIEASGKFVENNIASGKDRKKHE
Sbjct: 181 KKHKKKSEDRHAKIEDDERENGARRSYSKSRISDNNGEIEASGKFVENNIASGKDRKKHE 240

Query: 241 DKKSLVDDKDQVKSEGQRRRDAEEEKSTNKDNDDGAESTKKKKKKKKKKNREEEDDDFQN 300
           DKKSLVDDKDQVKSEGQRRRDAEEEKSTNKDNDDGAESTKKKKKKKKKKNREEEDDDFQN
Sbjct: 241 DKKSLVDDKDQVKSEGQRRRDAEEEKSTNKDNDDGAESTKKKKKKKKKKNREEEDDDFQN 300

Query: 301 NSGGAMVKEEIPVSDDKELKRKEKKKRKNRGLEEGGDDGSEEQQRTKRRKGNL 354
           NSGGAMVKEEIPVSDDKELKRKEKKKRKNRGLEEGGDDGSEEQQRTKRRKGNL
Sbjct: 301 NSGGAMVKEEIPVSDDKELKRKEKKKRKNRGLEEGGDDGSEEQQRTKRRKGNL 353

[Close]

BLAST of Carg03276 vs. NCBI nr
Match: XP_022943393.1 (glutamic acid-rich protein-like [Cucurbita moschata] >XP_022943394.1 glutamic acid-rich protein-like [Cucurbita moschata] >XP_022943395.1 glutamic acid-rich protein-like [Cucurbita moschata] >XP_022943397.1 glutamic acid-rich protein-like [Cucurbita moschata] >XP_022943398.1 glutamic acid-rich protein-like [Cucurbita moschata])

HSP 1 Score: 600.5 bits (1547), Expect = 9.3e-168
Identity = 342/353 (96.88%), Postives = 344/353 (97.45%), Query Frame = 0


Query: 1   MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS 60
           MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS
Sbjct: 1   MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS 60

Query: 61  SRSDRKHRHHGSEASNDPEASRDNPHWIEDGEKKNPLYLRAKVGRSGKPSFNVQSEDGKD 120
           SRSDRKHRHHGSEASNDPEASR NPHWIED EKKNPLYLRAK GRSGKPSFNVQSEDGKD
Sbjct: 61  SRSDRKHRHHGSEASNDPEASRGNPHWIEDDEKKNPLYLRAKDGRSGKPSFNVQSEDGKD 120

Query: 121 GKTEKESGGNGDFEDASGEYRKRKVEDLKTEIEDKPNRKVEMDVESSDKDKSVVAVETKR 180
           GKTEKESGG+GDFEDASGEYRKRKV DLKTEIEDKPNRKVEMDVESSDKDKSVVAVE K 
Sbjct: 121 GKTEKESGGSGDFEDASGEYRKRKVGDLKTEIEDKPNRKVEMDVESSDKDKSVVAVEKKG 180

Query: 181 KKHKKKSEDRHAKIEDDERENGARRSYSKSRISDNNGEIEASGKFVENNIASGKDRKKHE 240
           KKHKKKSEDRHAKIEDDERE+GARRSYSKSR SDNNGEIEASGKFVENNIASGKDRKKHE
Sbjct: 181 KKHKKKSEDRHAKIEDDEREDGARRSYSKSRNSDNNGEIEASGKFVENNIASGKDRKKHE 240

Query: 241 DKKSLVDDKDQVKSEGQRRRDAEEEKSTNKDNDDGAESTKKKKKKKKKKNREEEDDDFQN 300
           DKKSL DDKDQVKSEGQRRRDAEEEKSTNKDNDDG ESTKKKKKKKKKKNREEEDDDFQN
Sbjct: 241 DKKSLGDDKDQVKSEGQRRRDAEEEKSTNKDNDDGTESTKKKKKKKKKKNREEEDDDFQN 300

Query: 301 NSGGAMVKEEIPVSDDKELKRKEKKKRKNRGLEEGGDDGSEEQQRTKRRKGNL 354
           NSGGAMVKEEIPVSDDKELKRKEKKKRKNRGLEEGGDDGSEEQQRTKRRKGNL
Sbjct: 301 NSGGAMVKEEIPVSDDKELKRKEKKKRKNRGLEEGGDDGSEEQQRTKRRKGNL 353

[Close]

BLAST of Carg03276 vs. NCBI nr
Match: KAG6570745.1 (hypothetical protein SDJN03_29660, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 594.7 bits (1532), Expect = 5.1e-166
Identity = 335/339 (98.82%), Postives = 336/339 (99.12%), Query Frame = 0


Query: 1   MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS 60
           MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS
Sbjct: 1   MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS 60

Query: 61  SRSDRKHRHHGSEASNDPEASRDNPHWIEDGEKKNPLYLRAKVGRSGKPSFNVQSEDGKD 120
           SRSDRKHRHHGSEASNDPEASRDNPHWIEDGEKKNPLYLRAK GRSGKPSFNVQSEDGKD
Sbjct: 61  SRSDRKHRHHGSEASNDPEASRDNPHWIEDGEKKNPLYLRAKDGRSGKPSFNVQSEDGKD 120

Query: 121 GKTEKESGGNGDFEDASGEYRKRKVEDLKTEIEDKPNRKVEMDVESSDKDKSVVAVETKR 180
           GKTEKESGGNGDFEDASGEYRKRKVEDLKTEIE+KPNRKVEMDVESSDKDKSVVAVETKR
Sbjct: 121 GKTEKESGGNGDFEDASGEYRKRKVEDLKTEIENKPNRKVEMDVESSDKDKSVVAVETKR 180

Query: 181 KKHKKKSEDRHAKIEDDERENGARRSYSKSRISDNNGEIEASGKFVENNIASGKDRKKHE 240
           KKHKKKSEDRHAKIEDDERENGARRSYSKSRISDNNGEIEASGKFVENNIASGKDRKKHE
Sbjct: 181 KKHKKKSEDRHAKIEDDERENGARRSYSKSRISDNNGEIEASGKFVENNIASGKDRKKHE 240

Query: 241 DKKSLVDDKDQVKSEGQRRRDAEEEKSTNKDNDDGAESTKKKKKKKKKKNREEEDDDFQN 300
           DKKSL DDKDQVKSEGQRRRDAEEEKSTNKDNDDG ESTKKKKKKKKKKNREEEDDDFQN
Sbjct: 241 DKKSLGDDKDQVKSEGQRRRDAEEEKSTNKDNDDGTESTKKKKKKKKKKNREEEDDDFQN 300

Query: 301 NSGGAMVKEEIPVSDDKELKRKEKKKRKNRGLEEGGDDG 340
           NSGGAMVKEEIPVSDDKELKRKEKKKRKNRGLEEGGDDG
Sbjct: 301 NSGGAMVKEEIPVSDDKELKRKEKKKRKNRGLEEGGDDG 339

[Close]

BLAST of Carg03276 vs. NCBI nr
Match: XP_023511985.1 (DNA topoisomerase 1-like [Cucurbita pepo subsp. pepo] >XP_023511986.1 DNA topoisomerase 1-like [Cucurbita pepo subsp. pepo] >XP_023511987.1 DNA topoisomerase 1-like [Cucurbita pepo subsp. pepo] >XP_023511988.1 DNA topoisomerase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 582.8 bits (1501), Expect = 2.0e-162
Identity = 334/353 (94.62%), Postives = 338/353 (95.75%), Query Frame = 0


Query: 1   MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS 60
           MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS
Sbjct: 1   MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS 60

Query: 61  SRSDRKHRHHGSEASNDPEASRDNPHWIEDGEKKNPLYLRAKVGRSGKPSFNVQSEDGKD 120
           SRSDRKHRHHGSEASNDPEA R NPHWIEDGEKKN  YLR K GRSGKPS NVQSEDG+D
Sbjct: 61  SRSDRKHRHHGSEASNDPEAPRVNPHWIEDGEKKNLEYLREKDGRSGKPSLNVQSEDGQD 120

Query: 121 GKTEKESGGNGDFEDASGEYRKRKVEDLKTEIEDKPNRKVEMDVESSDKDKSVVAVETKR 180
           GKTE +SGGNGDFEDASGEYRKRKVEDLKTEIEDKPNRKVEMDVESSDKDKSVVAVE K 
Sbjct: 121 GKTETKSGGNGDFEDASGEYRKRKVEDLKTEIEDKPNRKVEMDVESSDKDKSVVAVEKKG 180

Query: 181 KKHKKKSEDRHAKIEDDERENGARRSYSKSRISDNNGEIEASGKFVENNIASGKDRKKHE 240
           KKHKKKSEDRHAKIEDDE E GARRSYSKSR SDNNGEIEASGKFVEN+IASGKDRKKHE
Sbjct: 181 KKHKKKSEDRHAKIEDDEHEAGARRSYSKSRNSDNNGEIEASGKFVENSIASGKDRKKHE 240

Query: 241 DKKSLVDDKDQVKSEGQRRRDAEEEKSTNKDNDDGAESTKKKKKKKKKKNREEEDDDFQN 300
           DKKSL DDKDQVKSEGQRRRDAEEEKSTNKDNDDG ESTKKK+KKKKKKNREEEDDDFQN
Sbjct: 241 DKKSLGDDKDQVKSEGQRRRDAEEEKSTNKDNDDGTESTKKKRKKKKKKNREEEDDDFQN 300

Query: 301 NSGGAMVKEEIPVSDDKELKRKEKKKRKNRGLEEGGDDGSEEQQRTKRRKGNL 354
           NSGGAMVKEEIPVSDDKELKRKEKKKRKNRGLEEGGDDGSEEQQRTKRRKGNL
Sbjct: 301 NSGGAMVKEEIPVSDDKELKRKEKKKRKNRGLEEGGDDGSEEQQRTKRRKGNL 353

[Close]

BLAST of Carg03276 vs. NCBI nr
Match: XP_022986894.1 (cylicin-1-like [Cucurbita maxima] >XP_022986895.1 cylicin-1-like [Cucurbita maxima] >XP_022986896.1 cylicin-1-like [Cucurbita maxima])

HSP 1 Score: 555.4 bits (1430), Expect = 3.4e-154
Identity = 325/354 (91.81%), Postives = 332/354 (93.79%), Query Frame = 0


Query: 1   MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS 60
           MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS
Sbjct: 1   MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS 60

Query: 61  SRSDRKHRHHGSEASNDPEASRDNPHWIEDGEKKNPLYLRAKVGRSGKPSFNVQSEDGKD 120
           SRSDRKHRHHGSEASNDPEA+RDNP WIEDGEKKNPLYLRAK G+SGK S NVQSE   D
Sbjct: 61  SRSDRKHRHHGSEASNDPEAARDNPQWIEDGEKKNPLYLRAKDGKSGKTSLNVQSE---D 120

Query: 121 GKTEKESGGNGDFEDASGEYRKRKVEDLKTEIEDKPNRKVEMDVESSDKDKSVVAVETKR 180
           GK EKESGGNGDFEDASGEYRKRKVEDLKTEIEDKPNRKVEMDVESSDKDKSVVAVE K 
Sbjct: 121 GKAEKESGGNGDFEDASGEYRKRKVEDLKTEIEDKPNRKVEMDVESSDKDKSVVAVEKKG 180

Query: 181 KKHKKKSEDRHAKIEDDERENGARRSYSKSRISDNNGEIEASGKFVENNIASGKDRKKHE 240
           KKH+KKSEDR+AKIEDDE + GARRS SKSR SDNNGEIEAS KFVENNIASGKDRKKH 
Sbjct: 181 KKHQKKSEDRYAKIEDDEHKAGARRSSSKSRNSDNNGEIEASAKFVENNIASGKDRKKHV 240

Query: 241 DKKSLVDDKDQVKSEGQRRRDAEEEKSTNKDNDDGAEST-KKKKKKKKKKNREEEDDDFQ 300
           DKKSL DDKDQVKSEG RRRDAEEEKSTNKDNDDG ES+ KKKKKKKKKKNREEEDDDFQ
Sbjct: 241 DKKSLGDDKDQVKSEGHRRRDAEEEKSTNKDNDDGTESSKKKKKKKKKKKNREEEDDDFQ 300

Query: 301 NNSGGAMVKEEIPVSDDKELKRKEKKKRKNRGLEEGGDDGSEEQQRTKRRKGNL 354
           NNSGGA+VKEEIPV DDKELKRKEKKKRKNR LEEGGDDGSEEQQRTKRRKGNL
Sbjct: 301 NNSGGALVKEEIPVLDDKELKRKEKKKRKNRDLEEGGDDGSEEQQRTKRRKGNL 351

[Close]

BLAST of Carg03276 vs. ExPASy TrEMBL
Match: A0A6J1FSX8 (glutamic acid-rich protein-like OS=Cucurbita moschata OX=3662 GN=LOC111448174 PE=4 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 4.5e-168
Identity = 342/353 (96.88%), Postives = 344/353 (97.45%), Query Frame = 0


Query: 1   MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS 60
           MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS
Sbjct: 1   MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS 60

Query: 61  SRSDRKHRHHGSEASNDPEASRDNPHWIEDGEKKNPLYLRAKVGRSGKPSFNVQSEDGKD 120
           SRSDRKHRHHGSEASNDPEASR NPHWIED EKKNPLYLRAK GRSGKPSFNVQSEDGKD
Sbjct: 61  SRSDRKHRHHGSEASNDPEASRGNPHWIEDDEKKNPLYLRAKDGRSGKPSFNVQSEDGKD 120

Query: 121 GKTEKESGGNGDFEDASGEYRKRKVEDLKTEIEDKPNRKVEMDVESSDKDKSVVAVETKR 180
           GKTEKESGG+GDFEDASGEYRKRKV DLKTEIEDKPNRKVEMDVESSDKDKSVVAVE K 
Sbjct: 121 GKTEKESGGSGDFEDASGEYRKRKVGDLKTEIEDKPNRKVEMDVESSDKDKSVVAVEKKG 180

Query: 181 KKHKKKSEDRHAKIEDDERENGARRSYSKSRISDNNGEIEASGKFVENNIASGKDRKKHE 240
           KKHKKKSEDRHAKIEDDERE+GARRSYSKSR SDNNGEIEASGKFVENNIASGKDRKKHE
Sbjct: 181 KKHKKKSEDRHAKIEDDEREDGARRSYSKSRNSDNNGEIEASGKFVENNIASGKDRKKHE 240

Query: 241 DKKSLVDDKDQVKSEGQRRRDAEEEKSTNKDNDDGAESTKKKKKKKKKKNREEEDDDFQN 300
           DKKSL DDKDQVKSEGQRRRDAEEEKSTNKDNDDG ESTKKKKKKKKKKNREEEDDDFQN
Sbjct: 241 DKKSLGDDKDQVKSEGQRRRDAEEEKSTNKDNDDGTESTKKKKKKKKKKNREEEDDDFQN 300

Query: 301 NSGGAMVKEEIPVSDDKELKRKEKKKRKNRGLEEGGDDGSEEQQRTKRRKGNL 354
           NSGGAMVKEEIPVSDDKELKRKEKKKRKNRGLEEGGDDGSEEQQRTKRRKGNL
Sbjct: 301 NSGGAMVKEEIPVSDDKELKRKEKKKRKNRGLEEGGDDGSEEQQRTKRRKGNL 353

[Close]

BLAST of Carg03276 vs. ExPASy TrEMBL
Match: A0A6J1JCJ7 (cylicin-1-like OS=Cucurbita maxima OX=3661 GN=LOC111484496 PE=4 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 1.7e-154
Identity = 325/354 (91.81%), Postives = 332/354 (93.79%), Query Frame = 0


Query: 1   MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS 60
           MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS
Sbjct: 1   MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS 60

Query: 61  SRSDRKHRHHGSEASNDPEASRDNPHWIEDGEKKNPLYLRAKVGRSGKPSFNVQSEDGKD 120
           SRSDRKHRHHGSEASNDPEA+RDNP WIEDGEKKNPLYLRAK G+SGK S NVQSE   D
Sbjct: 61  SRSDRKHRHHGSEASNDPEAARDNPQWIEDGEKKNPLYLRAKDGKSGKTSLNVQSE---D 120

Query: 121 GKTEKESGGNGDFEDASGEYRKRKVEDLKTEIEDKPNRKVEMDVESSDKDKSVVAVETKR 180
           GK EKESGGNGDFEDASGEYRKRKVEDLKTEIEDKPNRKVEMDVESSDKDKSVVAVE K 
Sbjct: 121 GKAEKESGGNGDFEDASGEYRKRKVEDLKTEIEDKPNRKVEMDVESSDKDKSVVAVEKKG 180

Query: 181 KKHKKKSEDRHAKIEDDERENGARRSYSKSRISDNNGEIEASGKFVENNIASGKDRKKHE 240
           KKH+KKSEDR+AKIEDDE + GARRS SKSR SDNNGEIEAS KFVENNIASGKDRKKH 
Sbjct: 181 KKHQKKSEDRYAKIEDDEHKAGARRSSSKSRNSDNNGEIEASAKFVENNIASGKDRKKHV 240

Query: 241 DKKSLVDDKDQVKSEGQRRRDAEEEKSTNKDNDDGAEST-KKKKKKKKKKNREEEDDDFQ 300
           DKKSL DDKDQVKSEG RRRDAEEEKSTNKDNDDG ES+ KKKKKKKKKKNREEEDDDFQ
Sbjct: 241 DKKSLGDDKDQVKSEGHRRRDAEEEKSTNKDNDDGTESSKKKKKKKKKKKNREEEDDDFQ 300

Query: 301 NNSGGAMVKEEIPVSDDKELKRKEKKKRKNRGLEEGGDDGSEEQQRTKRRKGNL 354
           NNSGGA+VKEEIPV DDKELKRKEKKKRKNR LEEGGDDGSEEQQRTKRRKGNL
Sbjct: 301 NNSGGALVKEEIPVLDDKELKRKEKKKRKNRDLEEGGDDGSEEQQRTKRRKGNL 351

[Close]

BLAST of Carg03276 vs. ExPASy TrEMBL
Match: A0A0A0KCS1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G301030 PE=4 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 4.9e-98
Identity = 242/358 (67.60%), Postives = 274/358 (76.54%), Query Frame = 0


Query: 1   MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS 60
           MKTV GS+VSSKPISISKAASTLSSFLS DNGASKA+CAYLRRAS SFNELKQLHKELKS
Sbjct: 1   MKTVNGSVVSSKPISISKAASTLSSFLSADNGASKALCAYLRRASDSFNELKQLHKELKS 60

Query: 61  SRSDRKHRHHGSEASNDPEASRDNPHWIEDGEKKNPLYLRAKV------GRSGKPSFNVQ 120
           S S RKH HHGSE SN+ EA+  + + +EDG+K N      K         + K S  VQ
Sbjct: 61  SCSVRKHLHHGSEVSNEFEAAIHDQYRVEDGDKNNSSVSEKKKRPDRKDRTTDKTSLRVQ 120

Query: 121 SEDGKDGKTEKESGGNGDFEDASGEYRKRKVEDLKTEIEDKPNRKVEMDVESSDKDKSVV 180
           S + + GKT  E+GGNG+ ED +G   K+K  +LK EIEDKP+ KVEMDVESSD+DKSVV
Sbjct: 121 SYNEQIGKTPMENGGNGNLEDVTG---KKKGSELKIEIEDKPSGKVEMDVESSDRDKSVV 180

Query: 181 AVETKRKKHKKKSEDRHAKIEDDERENGARRSYSKSRISDNNGEIEASGKFVENNIASGK 240
           AVE KRK+HKKKSEDRH  IEDDERE+GAR  + KS+ +DNN + EASG+FVENN+A+GK
Sbjct: 181 AVEKKRKRHKKKSEDRHDDIEDDERESGARLKHGKSQNTDNNCDAEASGEFVENNVANGK 240

Query: 241 DRKKHEDKKSLVDDKDQVKSEGQRRRDAEEEKSTNKDNDDGAESTKKKKKKKKKKNREEE 300
            RKK EDKK L D KDQVKSE QRR D +E KSTN DND+G +      KKKKK+ R EE
Sbjct: 241 SRKKLEDKKRLDDVKDQVKSEDQRRGDVKEGKSTNNDNDNGTDHVDLSPKKKKKR-RREE 300

Query: 301 DDDFQNNSGGAMVKEEIPVSDDKELKRKEKKKRKNRGL-EEGGDDGSEEQQRTKRRKG 352
           DDDFQ NSG AMVKEE+PV D KELKRKEKKK KNR L EEG DDGSEEQ  TKRRKG
Sbjct: 301 DDDFQKNSGEAMVKEEVPVLDSKELKRKEKKKSKNRELGEEGRDDGSEEQHSTKRRKG 354

[Close]

BLAST of Carg03276 vs. ExPASy TrEMBL
Match: A0A5A7SW64 (Glutamic acid-rich protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold228G00720 PE=4 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 1.1e-97
Identity = 242/359 (67.41%), Postives = 272/359 (75.77%), Query Frame = 0


Query: 1   MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS 60
           MKTVTGS+VSSKPISISKAASTLSSFLS DNGASKA+CAYLRRAS SFNELK LHKELKS
Sbjct: 1   MKTVTGSVVSSKPISISKAASTLSSFLSADNGASKALCAYLRRASDSFNELKHLHKELKS 60

Query: 61  SRSDRKHRHHGSEASNDPEASRDNPHWIEDGEKKNPLYLR------AKVGRSGKPSFNVQ 120
           S S RKH HHGS+ SN+ EA+ DN + +EDG+KKN           +K   + K S  VQ
Sbjct: 61  SPSVRKHLHHGSKVSNEFEAAMDNEYRVEDGDKKNSSVSEKKKRPDSKYRTTDKTSLRVQ 120

Query: 121 SEDGKDGKTEKESGGNGDFEDASGEYRKRKVEDLKTEIEDKPNRKVEMDVESSDKDKSVV 180
           S+D + GKT  E+GGNG+ ED SG   KRK   LK EIEDKP+ KVEMDVESSD    VV
Sbjct: 121 SDDEQSGKTAMENGGNGNLEDVSG---KRKGGGLKIEIEDKPSGKVEMDVESSD----VV 180

Query: 181 AVETKRKKHKKKSEDRHAKIEDDERENGARRSYSKSRISDNN-GEIEASGKFVENNIASG 240
           AVE KRKKHKKKSEDRH  IEDDERE+GAR  + KS+ +DNN    EASG+FVENN+A G
Sbjct: 181 AVEKKRKKHKKKSEDRHGDIEDDERESGARLKHGKSQNTDNNCDNAEASGEFVENNVAKG 240

Query: 241 KDRKKHEDKKSLVDDKDQVKSEGQRRRDAEEEKSTNKDNDDGAESTKKKKKKKKKKNREE 300
           K RKK EDK+SL D KDQVKSE QRR D +EE+ST+ DN +G +      KKKKK+ + E
Sbjct: 241 KSRKKLEDKRSLGDVKDQVKSEDQRRGDIKEERSTDNDNGNGTDLVDLSTKKKKKRKQRE 300

Query: 301 EDDDFQNNSGGAMVKEEIPVSDDKELKRKEKKKRKNRGL-EEGGDDGSEEQQRTKRRKG 352
           EDDDFQ NSGGAMVKEE+PV D KELKRKEKKK KNR L EEG DDGSEEQ   KRRKG
Sbjct: 301 EDDDFQKNSGGAMVKEEVPVLDSKELKRKEKKKSKNRELGEEGHDDGSEEQHSRKRRKG 352

[Close]

BLAST of Carg03276 vs. ExPASy TrEMBL
Match: A0A5D3CVE3 (Glutamic acid-rich protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00960 PE=4 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 9.2e-97
Identity = 241/359 (67.13%), Postives = 271/359 (75.49%), Query Frame = 0


Query: 1   MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS 60
           MKTVTGS+VSSKPISISKAASTLSSFLS DNGASKA+CAYLRRAS SFNELK LHKELKS
Sbjct: 1   MKTVTGSVVSSKPISISKAASTLSSFLSADNGASKALCAYLRRASDSFNELKHLHKELKS 60

Query: 61  SRSDRKHRHHGSEASNDPEASRDNPHWIEDGEKKNPLYLR------AKVGRSGKPSFNVQ 120
           S S RKH HHGS+ SN+ EA+ DN + +EDG+KKN           +K   + K S  VQ
Sbjct: 61  SPSVRKHLHHGSKVSNEFEAAMDNEYRVEDGDKKNSSVSEKKKRPDSKYRTTDKTSLRVQ 120

Query: 121 SEDGKDGKTEKESGGNGDFEDASGEYRKRKVEDLKTEIEDKPNRKVEMDVESSDKDKSVV 180
           S+D + GKT  E+GGNG+ ED SG   KRK   LK EIEDKP+ KVEMDVESSD    VV
Sbjct: 121 SDDEQSGKTAMENGGNGNLEDVSG---KRKGGGLKIEIEDKPSGKVEMDVESSD----VV 180

Query: 181 AVETKRKKHKKKSEDRHAKIEDDERENGARRSYSKSRISDNN-GEIEASGKFVENNIASG 240
           AVE KRKKHKKKSEDRH  IEDDERE+GAR  + KS+ +DNN    EASG+FVENN+A G
Sbjct: 181 AVEKKRKKHKKKSEDRHGDIEDDERESGARLKHGKSQNTDNNCDNAEASGEFVENNVAKG 240

Query: 241 KDRKKHEDKKSLVDDKDQVKSEGQRRRDAEEEKSTNKDNDDGAESTKKKKKKKKKKNREE 300
           K RKK EDK+SL D KDQVKSE QRR D +EE+ST+ DN +G +      KKKKK+ + E
Sbjct: 241 KSRKKLEDKRSLGDVKDQVKSEDQRRGDIKEERSTDNDNGNGTDLVDLSTKKKKKRKQRE 300

Query: 301 EDDDFQNNSGGAMVKEEIPVSDDKELKRKEKKKRKNRGL-EEGGDDGSEEQQRTKRRKG 352
           EDDDFQ NSG AMVKEE+PV D KELKRKEKKK KNR L EEG DDGSEEQ   KRRKG
Sbjct: 301 EDDDFQKNSGEAMVKEEVPVLDSKELKRKEKKKSKNRELGEEGHDDGSEEQHSRKRRKG 352

[Close]

BLAST of Carg03276 vs. TAIR 10
Match: AT1G75335.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G60030.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 71.2 bits (173), Expect = 1.8e-12
Identity = 47/89 (52.81%), Postives = 61/89 (68.54%), Query Frame = 0


Query: 1  MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELK- 60
          MKTVTG + S+KPIS+SKAA+ LS F+S +NGAS+ + AYLRRAS +F ELK +H+E+K 
Sbjct: 1  MKTVTGRVNSAKPISLSKAATLLSGFVSSENGASQDVSAYLRRASGAFIELKSIHREIKS 60

Query: 61 -----SSRSDRK-HRHHGSEASNDPEASR 83
               SS+  RK HR  GSE     +  R
Sbjct: 61 KETKLSSKKKRKSHREMGSEERKKSKKKR 89

[Close]

BLAST of Carg03276 vs. TAIR 10
Match: AT5G60030.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G75335.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 69.3 bits (168), Expect = 6.9e-12
Identity = 103/344 (29.94%), Postives = 176/344 (51.16%), Query Frame = 0


Query: 1   MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS 60
           MKTVTG +VS++PIS+SKAA  LS F S DNGAS+ + AYLRRASA+F ELK  H+E+KS
Sbjct: 1   MKTVTGRVVSAEPISLSKAAKLLSGFASSDNGASQDVSAYLRRASAAFTELKSFHREIKS 60

Query: 61  SR----SDRKHRHHGSEASNDPEASRDNPHWIEDGEKKNPLYLRAKVGRSGKPSFNVQSE 120
                 SDR+ +   ++ S+D ++ R   + I++ + +       K+      + +V+S 
Sbjct: 61  KETKPSSDRETKSTETKQSSDAKSER---NVIDEFDGR-------KIRYRNSEAVSVESV 120

Query: 121 DGKDGKTEKESGGNGDFEDASGEYRKRKVEDLKTEIEDKPNRKVEMDVESSDKDKSVVAV 180
            G++   +K               +K K  D+   +++K N K+E +  S ++ +     
Sbjct: 121 YGRERDEKK--------------MKKSKDADV---VDEKVNEKLEAEQRSEERRER--KK 180

Query: 181 ETKRKKHKKKSEDRHAKIE---DDERENGARRSYSKSRISDNNGEIEASGKFVENNIASG 240
           E K+KK+ K  +    K++   +DE+++  R+   K +   NN E          ++   
Sbjct: 181 EKKKKKNNKDEDVVDEKVKEKLEDEQKSADRKERKKKKSKKNNDE----------DVVDE 240

Query: 241 KDRKKHEDKKSLVDDKDQVKSEGQRRRDAEEEKSTNKDNDDGAESTKKKKKKKKKKNREE 300
           K++ + E K + + +K + K E     D  +EK   K  D+     +KK+KKKK+K+ EE
Sbjct: 241 KEKLEDEQKSAEIKEKKKNKDE-----DVVDEKEKEKLEDEQRSGERKKEKKKKRKSDEE 292

Query: 301 EDDDFQNNSGGAMVKEEIPVSDDKELKRKEKKKRKNRGLEEGGD 338
              + +        K +     D+E+  +E+K +K R L+E  D
Sbjct: 301 IVSEER--------KSKKKRKSDEEMGSEERKSKKKRKLKEIDD 292

The following BLAST results are available for this feature:

BLAST of Carg03276 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7010591.1	9.3e-176	100.00	hypothetical protein SDJN02_27385, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022943393.1	9.3e-168	96.88	glutamic acid-rich protein-like [Cucurbita moschata] >XP_022943394.1 glutamic ac...	[more]
KAG6570745.1	5.1e-166	98.82	hypothetical protein SDJN03_29660, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023511985.1	2.0e-162	94.62	DNA topoisomerase 1-like [Cucurbita pepo subsp. pepo] >XP_023511986.1 DNA topois...	[more]
XP_022986894.1	3.4e-154	91.81	cylicin-1-like [Cucurbita maxima] >XP_022986895.1 cylicin-1-like [Cucurbita maxi...	[more]

BLAST of Carg03276 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg03276 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FSX8	4.5e-168	96.88	glutamic acid-rich protein-like OS=Cucurbita moschata OX=3662 GN=LOC111448174 PE...	[more]
A0A6J1JCJ7	1.7e-154	91.81	cylicin-1-like OS=Cucurbita maxima OX=3661 GN=LOC111484496 PE=4 SV=1	[more]
A0A0A0KCS1	4.9e-98	67.60	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G301030 PE=4 SV=1	[more]
A0A5A7SW64	1.1e-97	67.41	Glutamic acid-rich protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]
A0A5D3CVE3	9.2e-97	67.13	Glutamic acid-rich protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]

BLAST of Carg03276 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G75335.1	1.8e-12	52.81	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT5G60030.1	6.9e-12	29.94	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 276..296
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 115..177
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 230..280
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 56..353
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 67..99
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 310..353
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 189..207
None	No IPR available	PANTHER	PTHR48227	DNA TOPOISOMERASE 1-LIKE	coord: 1..350

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg03276-RA	Carg03276-RA	mRNA