Carg03252 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg03252
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	vicilin-like seed storage protein At2g28490
Location	Carg_Chr20: 2329997 .. 2330491 (+)
RNA-Seq Expression	Carg03252
Synteny	Carg03252

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTCATGGAGGAATCTCATGAGTTCGATCTTTGGAAACGAAAATCGCTCAGAGGCACAGGCACAATACAAATCGTATATCCAAACGGAACCTCCGCCATGGACACAGAAGTAACCGAAGGGGACGTCTTCTGGGTTCCAAGATACTTCCCATTCTGCCAAATCGCATCGAGAACAGGTCCATTCGAGTTCTTCGGATTCACCACGTCGTCGCGCAAGAATCGACCGCAGTTCTTGGCCGGTGCGAATTCGATTTTCCACACTCTAAGAAGCCCGGCAGTGGCAACAGCATTCGACATAACAGAGGACGACCTCGACCGGTTGCTGAGCGCACAGTACGAGGTGGTGATTCTGCCGTCAGCGGAGATTGCGCCGCCGCACAAGGAGGAGGAGAAGAGGAGGAGGAGAGAGGAAGGAAGGAGGGAGAGGGAACGGGAAGGCGAAAGGGAACGGGAAAGGGAAGAAGAGTGGACGAGGCGATTTGGAGCGTTTTGA

mRNA sequence

ATGGTCATGGAGGAATCTCATGAGTTCGATCTTTGGAAACGAAAATCGCTCAGAGGCACAGGCACAATACAAATCGTATATCCAAACGGAACCTCCGCCATGGACACAGAAGTAACCGAAGGGGACGTCTTCTGGGTTCCAAGATACTTCCCATTCTGCCAAATCGCATCGAGAACAGGTCCATTCGAGTTCTTCGGATTCACCACGTCGTCGCGCAAGAATCGACCGCAGTTCTTGGCCGGTGCGAATTCGATTTTCCACACTCTAAGAAGCCCGGCAGTGGCAACAGCATTCGACATAACAGAGGACGACCTCGACCGGTTGCTGAGCGCACAGTACGAGGTGGTGATTCTGCCGTCAGCGGAGATTGCGCCGCCGCACAAGGAGGAGGAGAAGAGGAGGAGGAGAGAGGAAGGAAGGAGGGAGAGGGAACGGGAAGGCGAAAGGGAACGGGAAAGGGAAGAAGAGTGGACGAGGCGATTTGGAGCGTTTTGA

Coding sequence (CDS)

ATGGTCATGGAGGAATCTCATGAGTTCGATCTTTGGAAACGAAAATCGCTCAGAGGCACAGGCACAATACAAATCGTATATCCAAACGGAACCTCCGCCATGGACACAGAAGTAACCGAAGGGGACGTCTTCTGGGTTCCAAGATACTTCCCATTCTGCCAAATCGCATCGAGAACAGGTCCATTCGAGTTCTTCGGATTCACCACGTCGTCGCGCAAGAATCGACCGCAGTTCTTGGCCGGTGCGAATTCGATTTTCCACACTCTAAGAAGCCCGGCAGTGGCAACAGCATTCGACATAACAGAGGACGACCTCGACCGGTTGCTGAGCGCACAGTACGAGGTGGTGATTCTGCCGTCAGCGGAGATTGCGCCGCCGCACAAGGAGGAGGAGAAGAGGAGGAGGAGAGAGGAAGGAAGGAGGGAGAGGGAACGGGAAGGCGAAAGGGAACGGGAAAGGGAAGAAGAGTGGACGAGGCGATTTGGAGCGTTTTGA

Protein sequence

MVMEESHEFDLWKRKSLRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRPQFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRREEGRREREREGEREREREEEWTRRFGAF

Homology

[Close]

BLAST of Carg03252 vs. NCBI nr
Match: KAG7010615.1 (Vicilin-like seed storage protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 320.9 bits (821), Expect = 6.6e-84
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 1   MVMEESHEFDLWKRKSLRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTG 60
           MVMEESHEFDLWKRKSLRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTG
Sbjct: 1   MVMEESHEFDLWKRKSLRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTG 60

Query: 61  PFEFFGFTTSSRKNRPQFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPS 120
           PFEFFGFTTSSRKNRPQFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPS
Sbjct: 61  PFEFFGFTTSSRKNRPQFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPS 120

Query: 121 AEIAPPHKEEEKRRRREEGRREREREGEREREREEEWTRRFGAF 165
           AEIAPPHKEEEKRRRREEGRREREREGEREREREEEWTRRFGAF
Sbjct: 121 AEIAPPHKEEEKRRRREEGRREREREGEREREREEEWTRRFGAF 164

[Close]

BLAST of Carg03252 vs. NCBI nr
Match: KAG6570767.1 (Vicilin-like seed storage protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 286.6 bits (732), Expect = 1.4e-73
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 0


Query: 17  LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRP 76
           LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRP
Sbjct: 348 LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRP 407

Query: 77  QFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRR 136
           QFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRR
Sbjct: 408 QFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRR 467

Query: 137 EEGRREREREGEREREREEEWTRRFGAF 165
           EEGRREREREGEREREREEEWTRRFGAF
Sbjct: 468 EEGRREREREGEREREREEEWTRRFGAF 495

[Close]

BLAST of Carg03252 vs. NCBI nr
Match: XP_022943526.1 (vicilin-like seed storage protein At2g28490 [Cucurbita moschata])

HSP 1 Score: 280.0 bits (715), Expect = 1.3e-71
Identity = 145/148 (97.97%), Postives = 145/148 (97.97%), Query Frame = 0


Query: 17  LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRP 76
           LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRP
Sbjct: 348 LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRP 407

Query: 77  QFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRR 136
           QFLAGANSIFHTLRS AVATAFDITEDDLDRLLS QYE VILPSAEIAPPHKEEEKRRRR
Sbjct: 408 QFLAGANSIFHTLRSSAVATAFDITEDDLDRLLSLQYEAVILPSAEIAPPHKEEEKRRRR 467

Query: 137 EEGRREREREGEREREREEEWTRRFGAF 165
           EEGRREREREGEREREREEEWTRRFGAF
Sbjct: 468 EEGRREREREGEREREREEEWTRRFGAF 495

[Close]

BLAST of Carg03252 vs. NCBI nr
Match: XP_022985621.1 (vicilin-like seed storage protein At2g28490 [Cucurbita maxima])

HSP 1 Score: 270.4 bits (690), Expect = 1.0e-68
Identity = 142/148 (95.95%), Postives = 143/148 (96.62%), Query Frame = 0


Query: 17  LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRP 76
           LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSR+NRP
Sbjct: 354 LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRRNRP 413

Query: 77  QFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRR 136
           QFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRR
Sbjct: 414 QFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRR 473

Query: 137 EEGRREREREGEREREREEEWTRRFGAF 165
           EEGRRERERE   EREREEEWTRR  AF
Sbjct: 474 EEGRRERERES--EREREEEWTRRLEAF 499

[Close]

BLAST of Carg03252 vs. NCBI nr
Match: pir|T10443| (probable major protein body membrane protein MP27 / major protein body protein MP32 precursor - cucurbit >BAA06186.1 preproMP27-MP32 [Cucurbita hybrid cultivar])

HSP 1 Score: 266.9 bits (681), Expect = 1.1e-67
Identity = 141/148 (95.27%), Postives = 142/148 (95.95%), Query Frame = 0


Query: 17  LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRP 76
           LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSR+NRP
Sbjct: 354 LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRRNRP 413

Query: 77  QFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRR 136
           QFLA ANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRR
Sbjct: 414 QFLACANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRR 473

Query: 137 EEGRREREREGEREREREEEWTRRFGAF 165
           EEGRRERERE   EREREEEWTRR  AF
Sbjct: 474 EEGRRERERES--EREREEEWTRRLEAF 499

[Close]

BLAST of Carg03252 vs. ExPASy Swiss-Prot
Match: Q9SK09 (Vicilin-like seed storage protein At2g28490 OS=Arabidopsis thaliana OX=3702 GN=At2g28490 PE=2 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 5.3e-36
Identity = 70/114 (61.40%), Postives = 87/114 (76.32%), Query Frame = 0


Query: 17  LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRP 76
           L G+G IQ+V+PNGTSAM+T V+ GDVFW+PRYF FCQIASRTGPFEF GFTTS+ KNRP
Sbjct: 391 LAGSGEIQVVFPNGTSAMNTRVSVGDVFWIPRYFAFCQIASRTGPFEFVGFTTSAHKNRP 450

Query: 77  QFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEE 131
           QFL G+NS+  TL   +++ AF + E+ + R + AQ E VILP+   APPH  E
Sbjct: 451 QFLVGSNSLLRTLNLTSLSIAFGVDEETMRRFIEAQREAVILPTPAAAPPHVGE 504

[Close]

BLAST of Carg03252 vs. ExPASy Swiss-Prot
Match: F4IQK5 (Vicilin-like seed storage protein At2g18540 OS=Arabidopsis thaliana OX=3702 GN=At2g18540 PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 4.4e-14
Identity = 57/156 (36.54%), Postives = 84/156 (53.85%), Query Frame = 0


Query: 17  LRGTGTIQIVYPNGTSAMDTE-------VTEGDVFWVPRYFPFCQIASRTGPFEFFGFTT 76
           L G G +++V     S+   +       V EGDVF VP++ P  Q++     F F GF+T
Sbjct: 307 LEGEGMVRVVNQQSLSSCKNDRKSESFMVEEGDVFVVPKFHPMAQMSFENSSFVFMGFST 366

Query: 77  SSRKNRPQFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSA-----EIA 136
           S++ N PQFL G +S+   L    VA +F+++ + +  LL AQ E VI   A     E++
Sbjct: 367 SAKTNHPQFLVGQSSVLKVLDRDVVAVSFNLSNETIKGLLKAQKESVIFECASCAEGELS 426

Query: 137 PPHKEEEKRRRREEGRREREREGEREREREEEWTRR 161
              +E E+R+RREE   ER R+ E E  + EE  RR
Sbjct: 427 KLMREIEERKRREEEEIERRRKEEEEARKREEAKRR 462

[Close]

BLAST of Carg03252 vs. ExPASy Swiss-Prot
Match: F4JQG6 (Vicilin-like seed storage protein At4g36700 OS=Arabidopsis thaliana OX=3702 GN=At4g36700 PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 5.4e-12
Identity = 56/162 (34.57%), Postives = 84/162 (51.85%), Query Frame = 0


Query: 17  LRGTGTIQIVYPNGTSAMDTE-------VTEGDVFWVPRYFPFCQIASRTGPFEFFGFTT 76
           L+G G ++++  + +S   +E       V EGD+F VPR  P  Q++       F GFTT
Sbjct: 325 LKGAGMVRVLRSSISSNTSSECKNVRFKVEEGDIFAVPRLHPMAQMSFNNDSLVFVGFTT 384

Query: 77  SSRKNRPQFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILP---------- 136
           S++ N PQFLAG +S    L    +A + +++   +D LL AQ E VIL           
Sbjct: 385 SAKNNEPQFLAGEDSALRMLDRQVLAASLNVSSVTIDGLLGAQKEAVILECHSCAEGEIE 444

Query: 137 --SAEIAPPHKEEEKRRRREEGRRERE---REGEREREREEE 157
               EI     ++E++RR +E ++E E   RE E  R+REEE
Sbjct: 445 KLKVEIERKKIDDERKRRHDERKKEEEEAKREEEERRKREEE 486

[Close]

BLAST of Carg03252 vs. ExPASy Swiss-Prot
Match: P15590 (Globulin-1 S allele OS=Zea mays OX=4577 GN=GLB1 PE=1 SV=2)

HSP 1 Score: 58.5 bits (140), Expect = 8.0e-08
Identity = 45/131 (34.35%), Postives = 67/131 (51.15%), Query Frame = 0


Query: 30  GTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRPQFLAGANSIFHTL 89
           G   +   ++ G  F VP   PF  +ASR    +   F   + +N   FLAGA+++   L
Sbjct: 417 GYHTIRARLSPGTAFVVPAGHPFVAVASRDSNLQIVCFEVHADRNEKVFLAGADNVLQKL 476

Query: 90  RSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPH----KEEEKRRRREEGRRERER 149
              A A +F    +++D +L ++ E   LP  + +  H    +EEE+R  R  GR ERER
Sbjct: 477 DRVAKALSFASKAEEVDEVLGSRREKGFLPGPKESGGHEEREQEEEEREERHGGRGERER 536

Query: 150 EGEREREREEE 157
            G  ERE+EEE
Sbjct: 537 HGREEREKEEE 547

[Close]

BLAST of Carg03252 vs. ExPASy Swiss-Prot
Match: E3SH28 (Prunin 1 Pru du 6.0101 OS=Prunus dulcis OX=3755 PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 2.0e-06
Identity = 33/106 (31.13%), Postives = 51/106 (48.11%), Query Frame = 0


Query: 17  LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRP 76
           +RG   +Q+V  NG + +D EV +G +F VP+     Q A   G FE+F F T       
Sbjct: 442 IRGNARVQVVNENGDAILDQEVQQGQLFIVPQNHGVIQQAGNQG-FEYFAFKTEENA-FI 501

Query: 77  QFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAE 123
             LAG  S    L    +A A+ I+ +   +L   + E + L S++
Sbjct: 502 NTLAGRTSFLRALPDEVLANAYQISREQARQLKYNRQETIALSSSQ 545

[Close]

BLAST of Carg03252 vs. ExPASy TrEMBL
Match: A0A6J1FRY9 (vicilin-like seed storage protein At2g28490 OS=Cucurbita moschata OX=3662 GN=LOC111448275 PE=4 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 6.2e-72
Identity = 145/148 (97.97%), Postives = 145/148 (97.97%), Query Frame = 0


Query: 17  LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRP 76
           LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRP
Sbjct: 348 LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRP 407

Query: 77  QFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRR 136
           QFLAGANSIFHTLRS AVATAFDITEDDLDRLLS QYE VILPSAEIAPPHKEEEKRRRR
Sbjct: 408 QFLAGANSIFHTLRSSAVATAFDITEDDLDRLLSLQYEAVILPSAEIAPPHKEEEKRRRR 467

Query: 137 EEGRREREREGEREREREEEWTRRFGAF 165
           EEGRREREREGEREREREEEWTRRFGAF
Sbjct: 468 EEGRREREREGEREREREEEWTRRFGAF 495

[Close]

BLAST of Carg03252 vs. ExPASy TrEMBL
Match: A0A6J1J8R6 (vicilin-like seed storage protein At2g28490 OS=Cucurbita maxima OX=3661 GN=LOC111483630 PE=4 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 4.9e-69
Identity = 142/148 (95.95%), Postives = 143/148 (96.62%), Query Frame = 0


Query: 17  LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRP 76
           LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSR+NRP
Sbjct: 354 LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRRNRP 413

Query: 77  QFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRR 136
           QFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRR
Sbjct: 414 QFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRR 473

Query: 137 EEGRREREREGEREREREEEWTRRFGAF 165
           EEGRRERERE   EREREEEWTRR  AF
Sbjct: 474 EEGRRERERES--EREREEEWTRRLEAF 499

[Close]

BLAST of Carg03252 vs. ExPASy TrEMBL
Match: Q39651 (PreproMP27-MP32 OS=Cucurbita hybrid cultivar OX=170850 PE=2 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 5.5e-68
Identity = 141/148 (95.27%), Postives = 142/148 (95.95%), Query Frame = 0


Query: 17  LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRP 76
           LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSR+NRP
Sbjct: 354 LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRRNRP 413

Query: 77  QFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRR 136
           QFLA ANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRR
Sbjct: 414 QFLACANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRR 473

Query: 137 EEGRREREREGEREREREEEWTRRFGAF 165
           EEGRRERERE   EREREEEWTRR  AF
Sbjct: 474 EEGRRERERES--EREREEEWTRRLEAF 499

[Close]

BLAST of Carg03252 vs. ExPASy TrEMBL
Match: A0A5A7SVK6 (Vicilin-like seed storage protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold228G001130 PE=4 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 5.1e-58
Identity = 124/151 (82.12%), Postives = 134/151 (88.74%), Query Frame = 0


Query: 17  LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRP 76
           LRGTGTIQIVYPNGTSAM+ EVTEGDVFW+PRYFPFCQIASRTGPFEFFGFTTS+RKNRP
Sbjct: 298 LRGTGTIQIVYPNGTSAMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRP 357

Query: 77  QFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRR 136
           QFLAGA+SIFHTLRS  +ATAFDITEDD++RLL AQYE +ILPSAEIAPPHKEEEKRRR+
Sbjct: 358 QFLAGASSIFHTLRSMEMATAFDITEDDMERLLGAQYEAIILPSAEIAPPHKEEEKRRRK 417

Query: 137 EEGRREREREGERERERE-----EEWTRRFG 163
           EE RRE E E ERERERE     +E  R FG
Sbjct: 418 EEERREAEWERERERERERERRVDEMVRSFG 448

[Close]

BLAST of Carg03252 vs. ExPASy TrEMBL
Match: A0A5D3CSE7 (Vicilin-like seed storage protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00510 PE=4 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 5.1e-58
Identity = 124/151 (82.12%), Postives = 134/151 (88.74%), Query Frame = 0


Query: 17  LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRP 76
           LRGTGTIQIVYPNGTSAM+ EVTEGDVFW+PRYFPFCQIASRTGPFEFFGFTTS+RKNRP
Sbjct: 345 LRGTGTIQIVYPNGTSAMNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRP 404

Query: 77  QFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRR 136
           QFLAGA+SIFHTLRS  +ATAFDITEDD++RLL AQYE +ILPSAEIAPPHKEEEKRRR+
Sbjct: 405 QFLAGASSIFHTLRSMEMATAFDITEDDMERLLGAQYEAIILPSAEIAPPHKEEEKRRRK 464

Query: 137 EEGRREREREGERERERE-----EEWTRRFG 163
           EE RRE E E ERERERE     +E  R FG
Sbjct: 465 EEERREAEWERERERERERERRVDEMVRSFG 495

[Close]

BLAST of Carg03252 vs. TAIR 10
Match: AT2G28490.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 152.1 bits (383), Expect = 3.8e-37
Identity = 70/114 (61.40%), Postives = 87/114 (76.32%), Query Frame = 0


Query: 17  LRGTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRP 76
           L G+G IQ+V+PNGTSAM+T V+ GDVFW+PRYF FCQIASRTGPFEF GFTTS+ KNRP
Sbjct: 391 LAGSGEIQVVFPNGTSAMNTRVSVGDVFWIPRYFAFCQIASRTGPFEFVGFTTSAHKNRP 450

Query: 77  QFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEE 131
           QFL G+NS+  TL   +++ AF + E+ + R + AQ E VILP+   APPH  E
Sbjct: 451 QFLVGSNSLLRTLNLTSLSIAFGVDEETMRRFIEAQREAVILPTPAAAPPHVGE 504

[Close]

BLAST of Carg03252 vs. TAIR 10
Match: AT2G18540.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 79.3 bits (194), Expect = 3.1e-15
Identity = 57/156 (36.54%), Postives = 84/156 (53.85%), Query Frame = 0


Query: 17  LRGTGTIQIVYPNGTSAMDTE-------VTEGDVFWVPRYFPFCQIASRTGPFEFFGFTT 76
           L G G +++V     S+   +       V EGDVF VP++ P  Q++     F F GF+T
Sbjct: 307 LEGEGMVRVVNQQSLSSCKNDRKSESFMVEEGDVFVVPKFHPMAQMSFENSSFVFMGFST 366

Query: 77  SSRKNRPQFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSA-----EIA 136
           S++ N PQFL G +S+   L    VA +F+++ + +  LL AQ E VI   A     E++
Sbjct: 367 SAKTNHPQFLVGQSSVLKVLDRDVVAVSFNLSNETIKGLLKAQKESVIFECASCAEGELS 426

Query: 137 PPHKEEEKRRRREEGRREREREGEREREREEEWTRR 161
              +E E+R+RREE   ER R+ E E  + EE  RR
Sbjct: 427 KLMREIEERKRREEEEIERRRKEEEEARKREEAKRR 462

[Close]

BLAST of Carg03252 vs. TAIR 10
Match: AT4G36700.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 72.4 bits (176), Expect = 3.8e-13
Identity = 56/162 (34.57%), Postives = 84/162 (51.85%), Query Frame = 0


Query: 17  LRGTGTIQIVYPNGTSAMDTE-------VTEGDVFWVPRYFPFCQIASRTGPFEFFGFTT 76
           L+G G ++++  + +S   +E       V EGD+F VPR  P  Q++       F GFTT
Sbjct: 325 LKGAGMVRVLRSSISSNTSSECKNVRFKVEEGDIFAVPRLHPMAQMSFNNDSLVFVGFTT 384

Query: 77  SSRKNRPQFLAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILP---------- 136
           S++ N PQFLAG +S    L    +A + +++   +D LL AQ E VIL           
Sbjct: 385 SAKNNEPQFLAGEDSALRMLDRQVLAASLNVSSVTIDGLLGAQKEAVILECHSCAEGEIE 444

Query: 137 --SAEIAPPHKEEEKRRRREEGRRERE---REGEREREREEE 157
               EI     ++E++RR +E ++E E   RE E  R+REEE
Sbjct: 445 KLKVEIERKKIDDERKRRHDERKKEEEEAKREEEERRKREEE 486

[Close]

BLAST of Carg03252 vs. TAIR 10
Match: AT1G03890.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 44.3 bits (103), Expect = 1.1e-04
Identity = 25/99 (25.25%), Postives = 47/99 (47.47%), Query Frame = 0


Query: 19  GTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRPQF 78
           G   IQ+V  NG S  + +V +G +  +P+ F   + A  TG FE+  F T+        
Sbjct: 347 GQAKIQVVDDNGQSVFNEQVGQGQIIVIPQGFAVSKTAGETG-FEWISFKTNDNA-YINT 406

Query: 79  LAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVI 118
           L+G  S    +    +  ++ + E++  R+  +Q E ++
Sbjct: 407 LSGQTSYLRAVPVDVIKASYGVNEEEAKRIKFSQQETML 443

[Close]

BLAST of Carg03252 vs. TAIR 10
Match: AT4G28520.1 (cruciferin 3 )

HSP 1 Score: 43.9 bits (102), Expect = 1.4e-04
Identity = 26/103 (25.24%), Postives = 48/103 (46.60%), Query Frame = 0


Query: 19  GTGTIQIVYPNGTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRPQF 78
           G G IQ+V  NG + +D +V +G +  +P+ F +  + S    FE+  F T+        
Sbjct: 410 GQGRIQVVNDNGQNVLDQQVQKGQLVVIPQGFAYV-VQSHGNKFEWISFKTNENA-MIST 469

Query: 79  LAGANSIFHTLRSPAVATAFDITEDDLDRLLSAQYEVVILPSA 122
           LAG  S+   L    ++  F I+ ++  ++     E  +  +A
Sbjct: 470 LAGRTSLLRALPLEVISNGFQISPEEARKIKFNTLETTLTRAA 510

The following BLAST results are available for this feature:

BLAST of Carg03252 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7010615.1	6.6e-84	100.00	Vicilin-like seed storage protein, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6570767.1	1.4e-73	100.00	Vicilin-like seed storage protein, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022943526.1	1.3e-71	97.97	vicilin-like seed storage protein At2g28490 [Cucurbita moschata]	[more]
XP_022985621.1	1.0e-68	95.95	vicilin-like seed storage protein At2g28490 [Cucurbita maxima]	[more]
pir\|T10443\|	1.1e-67	95.27	probable major protein body membrane protein MP27 / major protein body protein M...	[more]

BLAST of Carg03252 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SK09	5.3e-36	61.40	Vicilin-like seed storage protein At2g28490 OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
F4IQK5	4.4e-14	36.54	Vicilin-like seed storage protein At2g18540 OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
F4JQG6	5.4e-12	34.57	Vicilin-like seed storage protein At4g36700 OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
P15590	8.0e-08	34.35	Globulin-1 S allele OS=Zea mays OX=4577 GN=GLB1 PE=1 SV=2	[more]
E3SH28	2.0e-06	31.13	Prunin 1 Pru du 6.0101 OS=Prunus dulcis OX=3755 PE=1 SV=1	[more]

BLAST of Carg03252 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FRY9	6.2e-72	97.97	vicilin-like seed storage protein At2g28490 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1J8R6	4.9e-69	95.95	vicilin-like seed storage protein At2g28490 OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
Q39651	5.5e-68	95.27	PreproMP27-MP32 OS=Cucurbita hybrid cultivar OX=170850 PE=2 SV=1	[more]
A0A5A7SVK6	5.1e-58	82.12	Vicilin-like seed storage protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A5D3CSE7	5.1e-58	82.12	Vicilin-like seed storage protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]

BLAST of Carg03252 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28490.1	3.8e-37	61.40	RmlC-like cupins superfamily protein	[more]
AT2G18540.1	3.1e-15	36.54	RmlC-like cupins superfamily protein	[more]
AT4G36700.1	3.8e-13	34.57	RmlC-like cupins superfamily protein	[more]
AT1G03890.1	1.1e-04	25.25	RmlC-like cupins superfamily protein	[more]
AT4G28520.1	1.4e-04	25.24	cruciferin 3	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006045	Cupin 1	SMART	SM00835	Cupin_1_3	coord: 6..106 e-value: 1.0E-9 score: 48.3
IPR006045	Cupin 1	PFAM	PF00190	Cupin_1	coord: 17..102 e-value: 6.5E-17 score: 61.5
IPR014710	RmlC-like jelly roll fold	GENE3D	2.60.120.10	Jelly Rolls	coord: 16..132 e-value: 2.8E-31 score: 110.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 124..164
None	No IPR available	PANTHER	PTHR31189:SF2	OS03G0336100 PROTEIN	coord: 17..128
None	No IPR available	PANTHER	PTHR31189	OS03G0336100 PROTEIN-RELATED	coord: 17..128
IPR011051	RmlC-like cupin domain superfamily	SUPERFAMILY	51182	RmlC-like cupins	coord: 17..120

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg03252-RA	Carg03252-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000272	polysaccharide catabolic process
molecular_function	GO:0102229	amylopectin maltohydrolase activity
molecular_function	GO:0016161	beta-amylase activity