Carg03213 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg03213
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Alpha-L-fucosidase
Location	Carg_Chr20: 2543915 .. 2545701 (+)
RNA-Seq Expression	Carg03213
Synteny	Carg03213

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAAGAAGCAGGCGATCAGGTTCTTCGGCTATTGCAATAATATCCCTAAGCCTTCTTCAGCTCTCAATTCAATTTCTCAAACCCAAATTTCCATTGTCTTCCTCCTCTCTCTGTTACTCCAACCCATTTCTTCTTCTTCTTCTTCGATTAGCTCTACCCCTCCGCTTCCCATTCTTCCCATCCCTTCCGCTTCTCAGATCCAATGGCAACTCGGAAACATGGCCCTTTTCCTTCACTTTGGCCCCAACACCTTCACCGATTCCGAATGGGGAACTGGCCACGTTGACCCATCGGCCTTCAACCCCACCAACCTCGATGCTGCTCAATGGGTCCGAGTCGCCAAGGACGCTGGGTTTTCCCGTTTGATTCTAACTGCCAAGCACCATGATGGGTTCTGCTTGTGGCCATCGGAGTATACAAATTACTCGGTACGGTCGAGTCCTTGGAGAATGGCGAAGGAGATGTGGTTGGGGAACTGGCCAAGGCTGCAAAGAAGCAGGACTTGGATTGGGACTCTATCTGTCGCCGTGGGATCGTCACGAGCCCTGTTATGGCAATACTCTGGAATATAATGAGTTCTATGTTGGCCAAATGACTGAATTGCTTACCAGGCACTGGTTCTTGGTTCCTTGTCTTTTAACAATTTGCTTGTTTCGCTAATTGAGTCAATTGGATAATGTTTTGATGCTGATTTGATCTTTTAGGTATGGAGAGATCAAGGAGGTGTGGTTGGATGGTGCAAAGGGAAAGGATGAGAAGGACATGGAATATTTCTTCGATTCATGGTTTAGTTTAATTCATCAGCTCCAACCGGGGGCTGTCATTTTCTCCGACGCCGGTCCCGACTGCCGGTGGATTGGCGACGAAGCTGGTGTCGCCAAGCCTACTTGCTGGTCTCTTTTTAACTGCAGTGCTGCAAAGATCGGCGGCACTGATTCCCGGTGAGTTCACTTCTCAATGTTAATGGCTTCTCTATCTCTGCTTGCTGTTTATTCCAACCTCTATTGTATTCAGATACTCTGGAGAAGGTGACCCATTTGGCCACGATTGGGTTCCAGCTGAGTGTGATGTCTCAATAAGGCCTGGTTGGTTCTGGCATCCATTAGAAGTTCCAAAGTCTGCAAGAACTCTTCTCGACATATATTACAAATCAGCGGGCAGGAACTGCCTCTTGCTGCTAAATGTACCTCCAAATTCCTCAGGTTTAATTTCAGCTGAAGATATACAAGTGCTGCAAGAATTCACCAAGATCCGAGACTCCATTTTCTCCCACAATTTGGCAGAAAAAGCTCTTGTCGACGCGAGCAGCACGCGAGGAAATGGTGAAGATGACCGCTTCATCCCCTCGAATGTCCTCAAAGAAGGTATCTACACTTACTGGGCTCCTGCTCAGAATCATCAACCCACTTGGTCTCTGTATCTGAACCTTCAAGAGTTCATATCTTTCAATGTGCTGCATATTCAAGAGCCGATTCACATGGGACAGAGGATTGTTGAGTTCCATTTTGATGTTCTGAATGATGAGGGTGTTTGGAGAACAGTGGTGAGAGGCTCCACAGTGGGTTACCGGAGAATACTGAAATTTCCAACCGTGGAATCTCAATTCTTGAGACTTGTTATTGAGAAATCAAGGGCTGATCCCCTCGTTTCTTATTTGGGGATTCATGTGGATAACTTCTCTCACTTGAGTAGTCATCTTGATAAGACATTGGGAGCAGGCATCAATGGCAGTAACATTCGTCGTCAGATTACCTTGAATAATTCTCAGATTTCTGCTGTATGA

mRNA sequence

ATGAAGAAGAAGCAGGCGATCAGGTTCTTCGGCTATTGCAATAATATCCCTAAGCCTTCTTCAGCTCTCAATTCAATTTCTCAAACCCAAATTTCCATTGTCTTCCTCCTCTCTCTGTTACTCCAACCCATTTCTTCTTCTTCTTCTTCGATTAGCTCTACCCCTCCGCTTCCCATTCTTCCCATCCCTTCCGCTTCTCAGATCCAATGGCAACTCGGAAACATGGCCCTTTTCCTTCACTTTGGCCCCAACACCTTCACCGATTCCGAATGGGGAACTGGCCACGTTGACCCATCGGCCTTCAACCCCACCAACCTCGATGCTGCTCAATGGGTCCGAGTCGCCAAGGACGCTGGGTTTTCCCGTTTGATTCTAACTGCCAAGCACCATGATGGGTTCTGCTTGTGGCCATCGGAGTATACAAATTACTCGGTACGGTCGAGTCCTTGGAGAATGGCGAAGGAGATGTGGTTGGGGAACTGGCCAAGGCTGCAAAGAAGCAGGACTTGGATTGGGACTCTATCTGTCGCCGTGGGATCGTCACGAGCCCTGTATGGAGAGATCAAGGAGGTGTGGTTGGATGGTGCAAAGGGAAAGGATGAGAAGGACATGGAATATTTCTTCGATTCATGGTTTAGTTTAATTCATCAGCTCCAACCGGGGGCTGTCATTTTCTCCGACGCCGGTCCCGACTGCCGGTGGATTGGCGACGAAGCTGGTGTCGCCAAGCCTACTTGCTGGTCTCTTTTTAACTGCAGTGCTGCAAAGATCGGCGGCACTGATTCCCGATACTCTGGAGAAGGTGACCCATTTGGCCACGATTGGGTTCCAGCTGAGTGTGATGTCTCAATAAGGCCTGGTTGGTTCTGGCATCCATTAGAAGTTCCAAAGTCTGCAAGAACTCTTCTCGACATATATTACAAATCAGCGGGCAGGAACTGCCTCTTGCTGCTAAATGTACCTCCAAATTCCTCAGGTTTAATTTCAGCTGAAGATATACAAGTGCTGCAAGAATTCACCAAGATCCGAGACTCCATTTTCTCCCACAATTTGGCAGAAAAAGCTCTTGTCGACGCGAGCAGCACGCGAGGAAATGGTGAAGATGACCGCTTCATCCCCTCGAATGTCCTCAAAGAAGGTATCTACACTTACTGGGCTCCTGCTCAGAATCATCAACCCACTTGGTCTCTGTATCTGAACCTTCAAGAGTTCATATCTTTCAATGTGCTGCATATTCAAGAGCCGATTCACATGGGACAGAGGATTGTTGAGTTCCATTTTGATGTTCTGAATGATGAGGGTGTTTGGAGAACAGTGGTGAGAGGCTCCACAGTGGGTTACCGGAGAATACTGAAATTTCCAACCGTGGAATCTCAATTCTTGAGACTTGTTATTGAGAAATCAAGGGCTGATCCCCTCGTTTCTTATTTGGGGATTCATGTGGATAACTTCTCTCACTTGAGTAGTCATCTTGATAAGACATTGGGAGCAGGCATCAATGGCAGTAACATTCGTCGTCAGATTACCTTGAATAATTCTCAGATTTCTGCTGTATGA

Coding sequence (CDS)

ATGAAGAAGAAGCAGGCGATCAGGTTCTTCGGCTATTGCAATAATATCCCTAAGCCTTCTTCAGCTCTCAATTCAATTTCTCAAACCCAAATTTCCATTGTCTTCCTCCTCTCTCTGTTACTCCAACCCATTTCTTCTTCTTCTTCTTCGATTAGCTCTACCCCTCCGCTTCCCATTCTTCCCATCCCTTCCGCTTCTCAGATCCAATGGCAACTCGGAAACATGGCCCTTTTCCTTCACTTTGGCCCCAACACCTTCACCGATTCCGAATGGGGAACTGGCCACGTTGACCCATCGGCCTTCAACCCCACCAACCTCGATGCTGCTCAATGGGTCCGAGTCGCCAAGGACGCTGGGTTTTCCCGTTTGATTCTAACTGCCAAGCACCATGATGGGTTCTGCTTGTGGCCATCGGAGTATACAAATTACTCGGTACGGTCGAGTCCTTGGAGAATGGCGAAGGAGATGTGGTTGGGGAACTGGCCAAGGCTGCAAAGAAGCAGGACTTGGATTGGGACTCTATCTGTCGCCGTGGGATCGTCACGAGCCCTGTATGGAGAGATCAAGGAGGTGTGGTTGGATGGTGCAAAGGGAAAGGATGAGAAGGACATGGAATATTTCTTCGATTCATGGTTTAGTTTAATTCATCAGCTCCAACCGGGGGCTGTCATTTTCTCCGACGCCGGTCCCGACTGCCGGTGGATTGGCGACGAAGCTGGTGTCGCCAAGCCTACTTGCTGGTCTCTTTTTAACTGCAGTGCTGCAAAGATCGGCGGCACTGATTCCCGATACTCTGGAGAAGGTGACCCATTTGGCCACGATTGGGTTCCAGCTGAGTGTGATGTCTCAATAAGGCCTGGTTGGTTCTGGCATCCATTAGAAGTTCCAAAGTCTGCAAGAACTCTTCTCGACATATATTACAAATCAGCGGGCAGGAACTGCCTCTTGCTGCTAAATGTACCTCCAAATTCCTCAGGTTTAATTTCAGCTGAAGATATACAAGTGCTGCAAGAATTCACCAAGATCCGAGACTCCATTTTCTCCCACAATTTGGCAGAAAAAGCTCTTGTCGACGCGAGCAGCACGCGAGGAAATGGTGAAGATGACCGCTTCATCCCCTCGAATGTCCTCAAAGAAGGTATCTACACTTACTGGGCTCCTGCTCAGAATCATCAACCCACTTGGTCTCTGTATCTGAACCTTCAAGAGTTCATATCTTTCAATGTGCTGCATATTCAAGAGCCGATTCACATGGGACAGAGGATTGTTGAGTTCCATTTTGATGTTCTGAATGATGAGGGTGTTTGGAGAACAGTGGTGAGAGGCTCCACAGTGGGTTACCGGAGAATACTGAAATTTCCAACCGTGGAATCTCAATTCTTGAGACTTGTTATTGAGAAATCAAGGGCTGATCCCCTCGTTTCTTATTTGGGGATTCATGTGGATAACTTCTCTCACTTGAGTAGTCATCTTGATAAGACATTGGGAGCAGGCATCAATGGCAGTAACATTCGTCGTCAGATTACCTTGAATAATTCTCAGATTTCTGCTGTATGA

Protein sequence

MKKKQAIRFFGYCNNIPKPSSALNSISQTQISIVFLLSLLLQPISSSSSSISSTPPLPILPIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHVDPSAFNPTNLDAAQWVRVAKDAGFSRLILTAKHHDGFCLWPSEYTNYSVRSSPWRMAKEMWLGNWPRLQRSRTWIGTLSVAVGSSRALYGEIKEVWLDGAKGKDEKDMEYFFDSWFSLIHQLQPGAVIFSDAGPDCRWIGDEAGVAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGHDWVPAECDVSIRPGWFWHPLEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDIQVLQEFTKIRDSIFSHNLAEKALVDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPTWSLYLNLQEFISFNVLHIQEPIHMGQRIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKFPTVESQFLRLVIEKSRADPLVSYLGIHVDNFSHLSSHLDKTLGAGINGSNIRRQITLNNSQISAV

Homology

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BLAST of Carg03213 vs. NCBI nr
Match: KAG7010653.1 (Alpha-L-fucosidase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1075.5 bits (2780), Expect = 0.0e+00
Identity = 518/518 (100.00%), Postives = 518/518 (100.00%), Query Frame = 0


Query: 1   MKKKQAIRFFGYCNNIPKPSSALNSISQTQISIVFLLSLLLQPISSSSSSISSTPPLPIL 60
           MKKKQAIRFFGYCNNIPKPSSALNSISQTQISIVFLLSLLLQPISSSSSSISSTPPLPIL
Sbjct: 1   MKKKQAIRFFGYCNNIPKPSSALNSISQTQISIVFLLSLLLQPISSSSSSISSTPPLPIL 60

Query: 61  PIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHVDPSAFNPTNLDAAQWVRVAKDAGF 120
           PIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHVDPSAFNPTNLDAAQWVRVAKDAGF
Sbjct: 61  PIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHVDPSAFNPTNLDAAQWVRVAKDAGF 120

Query: 121 SRLILTAKHHDGFCLWPSEYTNYSVRSSPWRMAKEMWLGNWPRLQRSRTWIGTLSVAVGS 180
           SRLILTAKHHDGFCLWPSEYTNYSVRSSPWRMAKEMWLGNWPRLQRSRTWIGTLSVAVGS
Sbjct: 121 SRLILTAKHHDGFCLWPSEYTNYSVRSSPWRMAKEMWLGNWPRLQRSRTWIGTLSVAVGS 180

Query: 181 SRALYGEIKEVWLDGAKGKDEKDMEYFFDSWFSLIHQLQPGAVIFSDAGPDCRWIGDEAG 240
           SRALYGEIKEVWLDGAKGKDEKDMEYFFDSWFSLIHQLQPGAVIFSDAGPDCRWIGDEAG
Sbjct: 181 SRALYGEIKEVWLDGAKGKDEKDMEYFFDSWFSLIHQLQPGAVIFSDAGPDCRWIGDEAG 240

Query: 241 VAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGHDWVPAECDVSIRPGWFWHPLEVPKSAR 300
           VAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGHDWVPAECDVSIRPGWFWHPLEVPKSAR
Sbjct: 241 VAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGHDWVPAECDVSIRPGWFWHPLEVPKSAR 300

Query: 301 TLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDIQVLQEFTKIRDSIFSHNLAEKALVDAS 360
           TLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDIQVLQEFTKIRDSIFSHNLAEKALVDAS
Sbjct: 301 TLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDIQVLQEFTKIRDSIFSHNLAEKALVDAS 360

Query: 361 STRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPTWSLYLNLQEFISFNVLHIQEPIHMGQ 420
           STRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPTWSLYLNLQEFISFNVLHIQEPIHMGQ
Sbjct: 361 STRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPTWSLYLNLQEFISFNVLHIQEPIHMGQ 420

Query: 421 RIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKFPTVESQFLRLVIEKSRADPLVSYLGIH 480
           RIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKFPTVESQFLRLVIEKSRADPLVSYLGIH
Sbjct: 421 RIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKFPTVESQFLRLVIEKSRADPLVSYLGIH 480

Query: 481 VDNFSHLSSHLDKTLGAGINGSNIRRQITLNNSQISAV 519
           VDNFSHLSSHLDKTLGAGINGSNIRRQITLNNSQISAV
Sbjct: 481 VDNFSHLSSHLDKTLGAGINGSNIRRQITLNNSQISAV 518

[Close]

BLAST of Carg03213 vs. NCBI nr
Match: KAG6570812.1 (Alpha-L-fucosidase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 993.4 bits (2567), Expect = 7.2e-286
Identity = 491/544 (90.26%), Postives = 500/544 (91.91%), Query Frame = 0


Query: 1   MKKKQAIRFFGYCNNIPKPSSALNSISQTQISIVFLLSLLLQPISSSSSSISSTPPLPIL 60
           MKKKQAIRFFGYCNNIPKPSSALNSISQTQISIVFLLSLLLQPISSSSSSISS PPLPIL
Sbjct: 1   MKKKQAIRFFGYCNNIPKPSSALNSISQTQISIVFLLSLLLQPISSSSSSISSPPPLPIL 60

Query: 61  PIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHVDPSAFNPTNLDAAQWVRVAKDAGF 120
           PIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHVDPSAFNPTNLDAAQWVRVAKDAGF
Sbjct: 61  PIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHVDPSAFNPTNLDAAQWVRVAKDAGF 120

Query: 121 SRLILTAKHHDGFCLWPSEYTNYSVRSSPWR------------MAKE------MWLGNWP 180
           SRLILTAKHHDGFCLWPSEYTNYSVRSSPWR             AKE      ++L  W 
Sbjct: 121 SRLILTAKHHDGFCLWPSEYTNYSVRSSPWRDGEGDVVGELAKAAKEAGLGLGLYLSPWD 180

Query: 181 R--------LQRSRTWIGTLSVAVGSSRALYGEIKEVWLDGAKGKDEKDMEYFFDSWFSL 240
           R        L+ +  ++G ++  +      YGEIKEVWLDGAKGKDEKDMEYFFDSWFSL
Sbjct: 181 RHEPCYGNTLEYNEFYVGQMTELL----TRYGEIKEVWLDGAKGKDEKDMEYFFDSWFSL 240

Query: 241 IHQLQPGAVIFSDAGPDCRWIGDEAGVAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGHD 300
           IHQLQPGAVIFSDAGPDCRWIGDEAGVAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGHD
Sbjct: 241 IHQLQPGAVIFSDAGPDCRWIGDEAGVAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGHD 300

Query: 301 WVPAECDVSIRPGWFWHPLEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDIQ 360
           WVPAECDVSIRPGWFWHPLEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDIQ
Sbjct: 301 WVPAECDVSIRPGWFWHPLEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDIQ 360

Query: 361 VLQEFTKIRDSIFSHNLAEKALVDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPT 420
           VLQEFTKIRDSIFSHNLAE ALVDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPT
Sbjct: 361 VLQEFTKIRDSIFSHNLAENALVDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPT 420

Query: 421 WSLYLNLQEFISFNVLHIQEPIHMGQRIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKFP 480
           WSLYLNLQEFISFNVLHIQEPIHMGQRIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKFP
Sbjct: 421 WSLYLNLQEFISFNVLHIQEPIHMGQRIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKFP 480

Query: 481 TVESQFLRLVIEKSRADPLVSYLGIHVDNFSHLSSHLDKTLGAGINGSNIRRQITLNNSQ 519
           TVESQFLRLVIEKSRADPLVSYLGIHVDNFSHLSSHLDKTLGAGINGSNIRRQITLNNSQ
Sbjct: 481 TVESQFLRLVIEKSRADPLVSYLGIHVDNFSHLSSHLDKTLGAGINGSNIRRQITLNNSQ 540

[Close]

BLAST of Carg03213 vs. NCBI nr
Match: XP_022944007.1 (alpha-L-fucosidase 1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 988.8 bits (2555), Expect = 1.8e-284
Identity = 488/544 (89.71%), Postives = 498/544 (91.54%), Query Frame = 0


Query: 1   MKKKQAIRFFGYCNNIPKPSSALNSISQTQISIVFLLSLLLQPISSSSSSISSTPPLPIL 60
           MKKK AIRFFGYCNNIPKPSSA+NSISQTQISIVFLLSLLLQPISSSSSSISS PPLPIL
Sbjct: 1   MKKKPAIRFFGYCNNIPKPSSAINSISQTQISIVFLLSLLLQPISSSSSSISSPPPLPIL 60

Query: 61  PIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHVDPSAFNPTNLDAAQWVRVAKDAGF 120
           PIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHVDPS FNPTNLDAAQWVRVAKDAGF
Sbjct: 61  PIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHVDPSVFNPTNLDAAQWVRVAKDAGF 120

Query: 121 SRLILTAKHHDGFCLWPSEYTNYSVRSSPWR------------MAKE------MWLGNWP 180
           SRLILTAKHHDGFCLWPSEYTNYSVRSSPWR             AKE      ++L  W 
Sbjct: 121 SRLILTAKHHDGFCLWPSEYTNYSVRSSPWRNGEGDVVGELAKAAKEAGLGLGLYLSPWD 180

Query: 181 R--------LQRSRTWIGTLSVAVGSSRALYGEIKEVWLDGAKGKDEKDMEYFFDSWFSL 240
           R        L+ +  ++G ++  +      YGEIKEVWLDGAKGKDEKDMEYFFDSWFSL
Sbjct: 181 RHEPCYGNTLEYNEFYVGQMTELL----TRYGEIKEVWLDGAKGKDEKDMEYFFDSWFSL 240

Query: 241 IHQLQPGAVIFSDAGPDCRWIGDEAGVAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGHD 300
           IHQLQPGAVIFSDAGPDCRWIGDEAGVAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGHD
Sbjct: 241 IHQLQPGAVIFSDAGPDCRWIGDEAGVAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGHD 300

Query: 301 WVPAECDVSIRPGWFWHPLEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDIQ 360
           WVPAECDVSIRPGWFWHPLEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDIQ
Sbjct: 301 WVPAECDVSIRPGWFWHPLEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDIQ 360

Query: 361 VLQEFTKIRDSIFSHNLAEKALVDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPT 420
           VLQEFTKIRDSIFSHNLAE ALVDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPT
Sbjct: 361 VLQEFTKIRDSIFSHNLAENALVDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPT 420

Query: 421 WSLYLNLQEFISFNVLHIQEPIHMGQRIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKFP 480
           WSLYLNLQEFISFNVLHIQEPIHMGQRIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKFP
Sbjct: 421 WSLYLNLQEFISFNVLHIQEPIHMGQRIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKFP 480

Query: 481 TVESQFLRLVIEKSRADPLVSYLGIHVDNFSHLSSHLDKTLGAGINGSNIRRQITLNNSQ 519
           TVESQFLRLVIEKSRADPLVSYLGIHVDNFSHLSSHLDKTLGAGINGSNIRRQITLNNSQ
Sbjct: 481 TVESQFLRLVIEKSRADPLVSYLGIHVDNFSHLSSHLDKTLGAGINGSNIRRQITLNNSQ 540

[Close]

BLAST of Carg03213 vs. NCBI nr
Match: XP_022985567.1 (alpha-L-fucosidase 1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 960.3 bits (2481), Expect = 6.8e-276
Identity = 477/545 (87.52%), Postives = 490/545 (89.91%), Query Frame = 0


Query: 1   MKKKQAIRFFGYCNNIPKPSSALNSISQTQISIVFLLSLLLQPI-SSSSSSISSTPPLPI 60
           MKKK AIRFF YCNNIPKPSSALNSISQTQISIVFLLSLLLQPI SSSSSSISS PPLPI
Sbjct: 1   MKKKPAIRFFCYCNNIPKPSSALNSISQTQISIVFLLSLLLQPISSSSSSSISSPPPLPI 60

Query: 61  LPIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHVDPSAFNPTNLDAAQWVRVAKDAG 120
           LPIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHVDPSAFNPTNLDAAQWVRVAKDAG
Sbjct: 61  LPIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHVDPSAFNPTNLDAAQWVRVAKDAG 120

Query: 121 FSRLILTAKHHDGFCLWPSEYTNYSVRSSPWR------------MAKE------MWLGNW 180
           FSRLILTAKHHDGFCLWPSEYTNYSVRSSPWR             AKE      ++L  W
Sbjct: 121 FSRLILTAKHHDGFCLWPSEYTNYSVRSSPWRDGEGDVVGELAKAAKEAGLGLGLYLSPW 180

Query: 181 PR--------LQRSRTWIGTLSVAVGSSRALYGEIKEVWLDGAKGKDEKDMEYFFDSWFS 240
            R         + +  ++G ++  +      YGEIKEVWLDGAKGKDEKDMEYFFDSWFS
Sbjct: 181 DRHEPCYGNTQEYNEFYVGQMTELL----TRYGEIKEVWLDGAKGKDEKDMEYFFDSWFS 240

Query: 241 LIHQLQPGAVIFSDAGPDCRWIGDEAGVAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGH 300
           LIHQLQPGAVIFSDAGPDCRWIGDEAGVAKPTCWSLFNCSA KIGGTDSRYSGEGDPFGH
Sbjct: 241 LIHQLQPGAVIFSDAGPDCRWIGDEAGVAKPTCWSLFNCSAVKIGGTDSRYSGEGDPFGH 300

Query: 301 DWVPAECDVSIRPGWFWHPLEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDI 360
           DWVPAECDVSIRPGWFWHP EVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSG ISAEDI
Sbjct: 301 DWVPAECDVSIRPGWFWHPSEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGSISAEDI 360

Query: 361 QVLQEFTKIRDSIFSHNLAEKALVDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQP 420
           QVLQEFTKIRDSIFSHNLAE AL+DASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQP
Sbjct: 361 QVLQEFTKIRDSIFSHNLAENALLDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQP 420

Query: 421 TWSLYLNLQEFISFNVLHIQEPIHMGQRIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKF 480
           TWSLYLNLQEFISFNVLHIQEPIHMGQRI EFHFD+LN+EGVWRTVVRGSTVGYRRILKF
Sbjct: 421 TWSLYLNLQEFISFNVLHIQEPIHMGQRIAEFHFDILNEEGVWRTVVRGSTVGYRRILKF 480

Query: 481 PTVESQFLRLVIEKSRADPLVSYLGIHVDNFSHLSSHLDKTLGAGINGSNIRRQITLNNS 519
           PTVESQFLRLVIEKSRADPLVSYLGIHVDNFSHLSS  DKTLGAGINGS+I RQITLNNS
Sbjct: 481 PTVESQFLRLVIEKSRADPLVSYLGIHVDNFSHLSSRCDKTLGAGINGSDILRQITLNNS 540

[Close]

BLAST of Carg03213 vs. NCBI nr
Match: XP_022944008.1 (alpha-L-fucosidase 1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 947.6 bits (2448), Expect = 4.5e-272
Identity = 469/527 (88.99%), Postives = 479/527 (90.89%), Query Frame = 0


Query: 18  KPSSALNSISQTQISIVFLLSLLLQPISSSSSSISSTPPLPILPIPSASQIQWQLGNMAL 77
           K   A+NSISQTQISIVFLLSLLLQPISSSSSSISS PPLPILPIPSASQIQWQLGNMAL
Sbjct: 2   KKKPAINSISQTQISIVFLLSLLLQPISSSSSSISSPPPLPILPIPSASQIQWQLGNMAL 61

Query: 78  FLHFGPNTFTDSEWGTGHVDPSAFNPTNLDAAQWVRVAKDAGFSRLILTAKHHDGFCLWP 137
           FLHFGPNTFTDSEWGTGHVDPS FNPTNLDAAQWVRVAKDAGFSRLILTAKHHDGFCLWP
Sbjct: 62  FLHFGPNTFTDSEWGTGHVDPSVFNPTNLDAAQWVRVAKDAGFSRLILTAKHHDGFCLWP 121

Query: 138 SEYTNYSVRSSPWR------------MAKE------MWLGNWPR--------LQRSRTWI 197
           SEYTNYSVRSSPWR             AKE      ++L  W R        L+ +  ++
Sbjct: 122 SEYTNYSVRSSPWRNGEGDVVGELAKAAKEAGLGLGLYLSPWDRHEPCYGNTLEYNEFYV 181

Query: 198 GTLSVAVGSSRALYGEIKEVWLDGAKGKDEKDMEYFFDSWFSLIHQLQPGAVIFSDAGPD 257
           G ++  +      YGEIKEVWLDGAKGKDEKDMEYFFDSWFSLIHQLQPGAVIFSDAGPD
Sbjct: 182 GQMTELL----TRYGEIKEVWLDGAKGKDEKDMEYFFDSWFSLIHQLQPGAVIFSDAGPD 241

Query: 258 CRWIGDEAGVAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGHDWVPAECDVSIRPGWFWH 317
           CRWIGDEAGVAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGHDWVPAECDVSIRPGWFWH
Sbjct: 242 CRWIGDEAGVAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGHDWVPAECDVSIRPGWFWH 301

Query: 318 PLEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDIQVLQEFTKIRDSIFSHNL 377
           PLEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDIQVLQEFTKIRDSIFSHNL
Sbjct: 302 PLEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDIQVLQEFTKIRDSIFSHNL 361

Query: 378 AEKALVDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPTWSLYLNLQEFISFNVLH 437
           AE ALVDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPTWSLYLNLQEFISFNVLH
Sbjct: 362 AENALVDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPTWSLYLNLQEFISFNVLH 421

Query: 438 IQEPIHMGQRIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKFPTVESQFLRLVIEKSRAD 497
           IQEPIHMGQRIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKFPTVESQFLRLVIEKSRAD
Sbjct: 422 IQEPIHMGQRIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKFPTVESQFLRLVIEKSRAD 481

Query: 498 PLVSYLGIHVDNFSHLSSHLDKTLGAGINGSNIRRQITLNNSQISAV 519
           PLVSYLGIHVDNFSHLSSHLDKTLGAGINGSNIRRQITLNNSQISAV
Sbjct: 482 PLVSYLGIHVDNFSHLSSHLDKTLGAGINGSNIRRQITLNNSQISAV 524

[Close]

BLAST of Carg03213 vs. ExPASy Swiss-Prot
Match: Q8GW72 (Alpha-L-fucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=FUC1 PE=1 SV=2)

HSP 1 Score: 586.6 bits (1511), Expect = 2.7e-166
Identity = 291/487 (59.75%), Postives = 359/487 (73.72%), Query Frame = 0


Query: 35  FLLSLLLQPISSSSSSISSTPPLPILPIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTG 94
           F  S+L     S+SSS+    P PILP+PS+ Q+QWQLG+MA+FLHFGPNTFTDSEWGTG
Sbjct: 10  FFFSILSLSQISNSSSLLKPHPCPILPLPSSQQLQWQLGSMAMFLHFGPNTFTDSEWGTG 69

Query: 95  HVDPSAFNPTNLDAAQWVRVAKDAGFSRLILTAKHHDGFCLWPSEYTNYSVRSSPWR--- 154
             +PS FNPT+L+A+QWV++AKD+GFSR+ILTAKHHDGFCLWPSEYT+YSV+SS WR   
Sbjct: 70  KANPSIFNPTHLNASQWVQIAKDSGFSRVILTAKHHDGFCLWPSEYTDYSVKSSQWRNGA 129

Query: 155 ---------MAKE------MWLGNWPR--------LQRSRTWIGTLSVAVGSSRALYGEI 214
                     AKE      ++L  W R        L+ +  ++  ++  +      YGEI
Sbjct: 130 GDVVAELASAAKEAGIGLGLYLSPWDRHEQCYGKTLEYNEFYLSQMTELL----TKYGEI 189

Query: 215 KEVWLDGAKGKDEKDMEYFFDSWFSLIHQLQPGAVIFSDAGPDCRWIGDEAGVAKPTCWS 274
           KEVWLDGAKG  EKDMEYFFD+WFSLIHQLQP AVIFSDAGPD RWIGDEAG+A  TCWS
Sbjct: 190 KEVWLDGAKGDGEKDMEYFFDTWFSLIHQLQPKAVIFSDAGPDVRWIGDEAGLAGSTCWS 249

Query: 275 LFNCSAAKIGGTDSRYSGEGDPFGHDWVPAECDVSIRPGWFWHPLEVPKSARTLLDIYYK 334
           LFN + AKIG T+  YS EGD +G DWVPAECDVSIRPGWFWH  E PK A  LLDIYY 
Sbjct: 250 LFNRTNAKIGDTEPSYSQEGDGYGQDWVPAECDVSIRPGWFWHASESPKPAVQLLDIYYN 309

Query: 335 SAGRNCLLLLNVPPNSSGLISAEDIQVLQEFTKIRDSIFSHNLAEKALVDASSTRGNGED 394
           S GRNCL LLNVPPNSSGLIS +DI+VL+EF+++++SIFS+NLA KA V++SS RG+ + 
Sbjct: 310 SVGRNCLFLLNVPPNSSGLISEQDIKVLEEFSEMKNSIFSNNLARKAFVNSSSIRGD-QS 369

Query: 395 DRFIPSNVLKEGIYTYWAPAQNHQPTWSLYLNLQEFISFNVLHIQEPIHMGQRIVEFHFD 454
            +F P NVL+EG+  YWAP +N Q  W LYL  ++ +SFNVL I+EPIHMGQRI  FH +
Sbjct: 370 SQFGPKNVLEEGLDKYWAPEEN-QNEWVLYLEFKDLVSFNVLEIREPIHMGQRIASFHLE 429

Query: 455 V-LNDEGVWRTVVRGSTVGYRRILKF-PTVESQFLRLVIEKSRADPLVSYLGIHVDNFSH 494
                 G W  VV G+TVG +R+L+F   VES+ L+LV++K+R DPL+SYLG+++D FS 
Sbjct: 430 TRKTGSGEWERVVSGTTVGNKRLLRFLNVVESRSLKLVVDKARTDPLISYLGLYMDKFSG 489

[Close]

BLAST of Carg03213 vs. ExPASy Swiss-Prot
Match: Q7XUR3 (Putative alpha-L-fucosidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0560400 PE=3 SV=2)

HSP 1 Score: 550.1 bits (1416), Expect = 2.8e-155
Identity = 266/491 (54.18%), Postives = 340/491 (69.25%), Query Frame = 0


Query: 40  LLQPISSSSSSISSTPPLPILPIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHVDPS 99
           LL+    + S+  + PPLP+LP+PS +Q+QWQL  MALFLHFGPNTFTDSEWG+   DP+
Sbjct: 17  LLRAHGVTGSAAPTPPPLPVLPVPSYAQLQWQLSEMALFLHFGPNTFTDSEWGSVRADPA 76

Query: 100 AFNPTNLDAAQWVRVAKDAGFSRLILTAKHHDGFCLWPSEYTNYSVRSSPWR-------- 159
            F P+ LDA QW R A   GF R++LTAKHHDGFCLWPS  TNYSV +SPW+        
Sbjct: 77  VFAPSALDAGQWARAAAAGGFGRVVLTAKHHDGFCLWPSALTNYSVAASPWKGGAGDVVG 136

Query: 160 ----------MAKEMWLGNWPR--------LQRSRTWIGTLSVAVGSSRALYGEIKEVWL 219
                     +   ++L  W R        +  +  ++G ++  +      YG+++EVWL
Sbjct: 137 ELAAAARAEGIGLGLYLSPWDRHEPVYGDTVAYNEHYLGQMTELL----TRYGDVEEVWL 196

Query: 220 DGAKGKDEKDMEYFFDSWFSLIHQLQPGAVIFSDAGPDCRWIGDEAGVAKPTCWSLFNCS 279
           DGAKG + KDM+Y FD+WF+LIHQLQ   VIFSDAGPD RW+GDEAGVA  TCWS FN S
Sbjct: 197 DGAKG-EGKDMDYMFDAWFALIHQLQQRVVIFSDAGPDTRWVGDEAGVAGYTCWSPFNKS 256

Query: 280 AAKIGGTDSRYSGEGDPFGHDWVPAECDVSIRPGWFWHPLEVPKSARTLLDIYYKSAGRN 339
              IG     YS  GDPFG DWVPAECDVSIRPGWFWH  E PK+A TLLDIYYKS GRN
Sbjct: 257 TVTIGHIIPEYSRCGDPFGQDWVPAECDVSIRPGWFWHASEKPKNATTLLDIYYKSVGRN 316

Query: 340 CLLLLNVPPNSSGLISAEDIQVLQEFTKIRDSIFSHNLAEKALVDASSTRGNGEDDRFIP 399
           CLL+LNVPPNSSGLIS ED+QVLQEFT+IR +IFS N A  A V AS+ RG   + +F P
Sbjct: 317 CLLILNVPPNSSGLISTEDMQVLQEFTEIRQTIFSQNFAANATVTASTVRGGLGNQQFAP 376

Query: 400 SNVLKEGIYTYWAPAQNHQPTWSLYLNLQEFISFNVLHIQEPIHMGQRIVEFHFDVLNDE 459
           SNVL+E IY+YWAP +  Q +W +  +L +  SFNV+ +QEPI MGQR+++F  ++L DE
Sbjct: 377 SNVLQESIYSYWAPEEG-QSSWEMLFDLGQSASFNVIQLQEPIQMGQRVIKFRVEILVDE 436

Query: 460 GVWRTVVRGSTVGYRRILKFPTVESQFLRLVIEKSRADPLVSYLGIHVDNFSHLSSHLDK 505
            +W+T+V G+T+GY+R+ +FP VE QFL+L I+ +RADPL+S+ G+  D+FS   S  + 
Sbjct: 437 -LWQTIVEGTTIGYKRLFQFPVVEGQFLKLSIDGARADPLISFFGVFTDSFSVTYSLENH 496

[Close]

BLAST of Carg03213 vs. ExPASy Swiss-Prot
Match: Q9VTJ4 (Putative alpha-L-fucosidase OS=Drosophila melanogaster OX=7227 GN=Fuca PE=2 SV=2)

HSP 1 Score: 48.9 bits (115), Expect = 2.0e-04
Identity = 19/41 (46.34%), Postives = 27/41 (65.85%), Query Frame = 0


Query: 99  SAFNPTNLDAAQWVRVAKDAGFSRLILTAKHHDGFCLWPSE 140
           S F     +A +W  + KD+G   ++LT+KHHDGF LWPS+
Sbjct: 105 SQFTAELFNATKWALLFKDSGARYVVLTSKHHDGFTLWPSK 145

[Close]

BLAST of Carg03213 vs. ExPASy TrEMBL
Match: A0A6J1FUL0 (Alpha-L-fucosidase OS=Cucurbita moschata OX=3662 GN=LOC111448567 PE=3 SV=1)

HSP 1 Score: 988.8 bits (2555), Expect = 8.6e-285
Identity = 488/544 (89.71%), Postives = 498/544 (91.54%), Query Frame = 0


Query: 1   MKKKQAIRFFGYCNNIPKPSSALNSISQTQISIVFLLSLLLQPISSSSSSISSTPPLPIL 60
           MKKK AIRFFGYCNNIPKPSSA+NSISQTQISIVFLLSLLLQPISSSSSSISS PPLPIL
Sbjct: 1   MKKKPAIRFFGYCNNIPKPSSAINSISQTQISIVFLLSLLLQPISSSSSSISSPPPLPIL 60

Query: 61  PIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHVDPSAFNPTNLDAAQWVRVAKDAGF 120
           PIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHVDPS FNPTNLDAAQWVRVAKDAGF
Sbjct: 61  PIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHVDPSVFNPTNLDAAQWVRVAKDAGF 120

Query: 121 SRLILTAKHHDGFCLWPSEYTNYSVRSSPWR------------MAKE------MWLGNWP 180
           SRLILTAKHHDGFCLWPSEYTNYSVRSSPWR             AKE      ++L  W 
Sbjct: 121 SRLILTAKHHDGFCLWPSEYTNYSVRSSPWRNGEGDVVGELAKAAKEAGLGLGLYLSPWD 180

Query: 181 R--------LQRSRTWIGTLSVAVGSSRALYGEIKEVWLDGAKGKDEKDMEYFFDSWFSL 240
           R        L+ +  ++G ++  +      YGEIKEVWLDGAKGKDEKDMEYFFDSWFSL
Sbjct: 181 RHEPCYGNTLEYNEFYVGQMTELL----TRYGEIKEVWLDGAKGKDEKDMEYFFDSWFSL 240

Query: 241 IHQLQPGAVIFSDAGPDCRWIGDEAGVAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGHD 300
           IHQLQPGAVIFSDAGPDCRWIGDEAGVAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGHD
Sbjct: 241 IHQLQPGAVIFSDAGPDCRWIGDEAGVAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGHD 300

Query: 301 WVPAECDVSIRPGWFWHPLEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDIQ 360
           WVPAECDVSIRPGWFWHPLEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDIQ
Sbjct: 301 WVPAECDVSIRPGWFWHPLEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDIQ 360

Query: 361 VLQEFTKIRDSIFSHNLAEKALVDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPT 420
           VLQEFTKIRDSIFSHNLAE ALVDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPT
Sbjct: 361 VLQEFTKIRDSIFSHNLAENALVDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPT 420

Query: 421 WSLYLNLQEFISFNVLHIQEPIHMGQRIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKFP 480
           WSLYLNLQEFISFNVLHIQEPIHMGQRIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKFP
Sbjct: 421 WSLYLNLQEFISFNVLHIQEPIHMGQRIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKFP 480

Query: 481 TVESQFLRLVIEKSRADPLVSYLGIHVDNFSHLSSHLDKTLGAGINGSNIRRQITLNNSQ 519
           TVESQFLRLVIEKSRADPLVSYLGIHVDNFSHLSSHLDKTLGAGINGSNIRRQITLNNSQ
Sbjct: 481 TVESQFLRLVIEKSRADPLVSYLGIHVDNFSHLSSHLDKTLGAGINGSNIRRQITLNNSQ 540

[Close]

BLAST of Carg03213 vs. ExPASy TrEMBL
Match: A0A6J1J578 (Alpha-L-fucosidase OS=Cucurbita maxima OX=3661 GN=LOC111483599 PE=3 SV=1)

HSP 1 Score: 960.3 bits (2481), Expect = 3.3e-276
Identity = 477/545 (87.52%), Postives = 490/545 (89.91%), Query Frame = 0


Query: 1   MKKKQAIRFFGYCNNIPKPSSALNSISQTQISIVFLLSLLLQPI-SSSSSSISSTPPLPI 60
           MKKK AIRFF YCNNIPKPSSALNSISQTQISIVFLLSLLLQPI SSSSSSISS PPLPI
Sbjct: 1   MKKKPAIRFFCYCNNIPKPSSALNSISQTQISIVFLLSLLLQPISSSSSSSISSPPPLPI 60

Query: 61  LPIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHVDPSAFNPTNLDAAQWVRVAKDAG 120
           LPIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHVDPSAFNPTNLDAAQWVRVAKDAG
Sbjct: 61  LPIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHVDPSAFNPTNLDAAQWVRVAKDAG 120

Query: 121 FSRLILTAKHHDGFCLWPSEYTNYSVRSSPWR------------MAKE------MWLGNW 180
           FSRLILTAKHHDGFCLWPSEYTNYSVRSSPWR             AKE      ++L  W
Sbjct: 121 FSRLILTAKHHDGFCLWPSEYTNYSVRSSPWRDGEGDVVGELAKAAKEAGLGLGLYLSPW 180

Query: 181 PR--------LQRSRTWIGTLSVAVGSSRALYGEIKEVWLDGAKGKDEKDMEYFFDSWFS 240
            R         + +  ++G ++  +      YGEIKEVWLDGAKGKDEKDMEYFFDSWFS
Sbjct: 181 DRHEPCYGNTQEYNEFYVGQMTELL----TRYGEIKEVWLDGAKGKDEKDMEYFFDSWFS 240

Query: 241 LIHQLQPGAVIFSDAGPDCRWIGDEAGVAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGH 300
           LIHQLQPGAVIFSDAGPDCRWIGDEAGVAKPTCWSLFNCSA KIGGTDSRYSGEGDPFGH
Sbjct: 241 LIHQLQPGAVIFSDAGPDCRWIGDEAGVAKPTCWSLFNCSAVKIGGTDSRYSGEGDPFGH 300

Query: 301 DWVPAECDVSIRPGWFWHPLEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDI 360
           DWVPAECDVSIRPGWFWHP EVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSG ISAEDI
Sbjct: 301 DWVPAECDVSIRPGWFWHPSEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGSISAEDI 360

Query: 361 QVLQEFTKIRDSIFSHNLAEKALVDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQP 420
           QVLQEFTKIRDSIFSHNLAE AL+DASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQP
Sbjct: 361 QVLQEFTKIRDSIFSHNLAENALLDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQP 420

Query: 421 TWSLYLNLQEFISFNVLHIQEPIHMGQRIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKF 480
           TWSLYLNLQEFISFNVLHIQEPIHMGQRI EFHFD+LN+EGVWRTVVRGSTVGYRRILKF
Sbjct: 421 TWSLYLNLQEFISFNVLHIQEPIHMGQRIAEFHFDILNEEGVWRTVVRGSTVGYRRILKF 480

Query: 481 PTVESQFLRLVIEKSRADPLVSYLGIHVDNFSHLSSHLDKTLGAGINGSNIRRQITLNNS 519
           PTVESQFLRLVIEKSRADPLVSYLGIHVDNFSHLSS  DKTLGAGINGS+I RQITLNNS
Sbjct: 481 PTVESQFLRLVIEKSRADPLVSYLGIHVDNFSHLSSRCDKTLGAGINGSDILRQITLNNS 540

[Close]

BLAST of Carg03213 vs. ExPASy TrEMBL
Match: A0A6J1FXK9 (Alpha-L-fucosidase OS=Cucurbita moschata OX=3662 GN=LOC111448567 PE=3 SV=1)

HSP 1 Score: 947.6 bits (2448), Expect = 2.2e-272
Identity = 469/527 (88.99%), Postives = 479/527 (90.89%), Query Frame = 0


Query: 18  KPSSALNSISQTQISIVFLLSLLLQPISSSSSSISSTPPLPILPIPSASQIQWQLGNMAL 77
           K   A+NSISQTQISIVFLLSLLLQPISSSSSSISS PPLPILPIPSASQIQWQLGNMAL
Sbjct: 2   KKKPAINSISQTQISIVFLLSLLLQPISSSSSSISSPPPLPILPIPSASQIQWQLGNMAL 61

Query: 78  FLHFGPNTFTDSEWGTGHVDPSAFNPTNLDAAQWVRVAKDAGFSRLILTAKHHDGFCLWP 137
           FLHFGPNTFTDSEWGTGHVDPS FNPTNLDAAQWVRVAKDAGFSRLILTAKHHDGFCLWP
Sbjct: 62  FLHFGPNTFTDSEWGTGHVDPSVFNPTNLDAAQWVRVAKDAGFSRLILTAKHHDGFCLWP 121

Query: 138 SEYTNYSVRSSPWR------------MAKE------MWLGNWPR--------LQRSRTWI 197
           SEYTNYSVRSSPWR             AKE      ++L  W R        L+ +  ++
Sbjct: 122 SEYTNYSVRSSPWRNGEGDVVGELAKAAKEAGLGLGLYLSPWDRHEPCYGNTLEYNEFYV 181

Query: 198 GTLSVAVGSSRALYGEIKEVWLDGAKGKDEKDMEYFFDSWFSLIHQLQPGAVIFSDAGPD 257
           G ++  +      YGEIKEVWLDGAKGKDEKDMEYFFDSWFSLIHQLQPGAVIFSDAGPD
Sbjct: 182 GQMTELL----TRYGEIKEVWLDGAKGKDEKDMEYFFDSWFSLIHQLQPGAVIFSDAGPD 241

Query: 258 CRWIGDEAGVAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGHDWVPAECDVSIRPGWFWH 317
           CRWIGDEAGVAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGHDWVPAECDVSIRPGWFWH
Sbjct: 242 CRWIGDEAGVAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGHDWVPAECDVSIRPGWFWH 301

Query: 318 PLEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDIQVLQEFTKIRDSIFSHNL 377
           PLEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDIQVLQEFTKIRDSIFSHNL
Sbjct: 302 PLEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDIQVLQEFTKIRDSIFSHNL 361

Query: 378 AEKALVDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPTWSLYLNLQEFISFNVLH 437
           AE ALVDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPTWSLYLNLQEFISFNVLH
Sbjct: 362 AENALVDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPTWSLYLNLQEFISFNVLH 421

Query: 438 IQEPIHMGQRIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKFPTVESQFLRLVIEKSRAD 497
           IQEPIHMGQRIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKFPTVESQFLRLVIEKSRAD
Sbjct: 422 IQEPIHMGQRIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKFPTVESQFLRLVIEKSRAD 481

Query: 498 PLVSYLGIHVDNFSHLSSHLDKTLGAGINGSNIRRQITLNNSQISAV 519
           PLVSYLGIHVDNFSHLSSHLDKTLGAGINGSNIRRQITLNNSQISAV
Sbjct: 482 PLVSYLGIHVDNFSHLSSHLDKTLGAGINGSNIRRQITLNNSQISAV 524

[Close]

BLAST of Carg03213 vs. ExPASy TrEMBL
Match: A0A6J1JDZ9 (Alpha-L-fucosidase OS=Cucurbita maxima OX=3661 GN=LOC111483599 PE=3 SV=1)

HSP 1 Score: 921.8 bits (2381), Expect = 1.3e-264
Identity = 458/528 (86.74%), Postives = 472/528 (89.39%), Query Frame = 0


Query: 18  KPSSALNSISQTQISIVFLLSLLLQPI-SSSSSSISSTPPLPILPIPSASQIQWQLGNMA 77
           K   A+NSISQTQISIVFLLSLLLQPI SSSSSSISS PPLPILPIPSASQIQWQLGNMA
Sbjct: 2   KKKPAINSISQTQISIVFLLSLLLQPISSSSSSSISSPPPLPILPIPSASQIQWQLGNMA 61

Query: 78  LFLHFGPNTFTDSEWGTGHVDPSAFNPTNLDAAQWVRVAKDAGFSRLILTAKHHDGFCLW 137
           LFLHFGPNTFTDSEWGTGHVDPSAFNPTNLDAAQWVRVAKDAGFSRLILTAKHHDGFCLW
Sbjct: 62  LFLHFGPNTFTDSEWGTGHVDPSAFNPTNLDAAQWVRVAKDAGFSRLILTAKHHDGFCLW 121

Query: 138 PSEYTNYSVRSSPWR------------MAKE------MWLGNWPR--------LQRSRTW 197
           PSEYTNYSVRSSPWR             AKE      ++L  W R         + +  +
Sbjct: 122 PSEYTNYSVRSSPWRDGEGDVVGELAKAAKEAGLGLGLYLSPWDRHEPCYGNTQEYNEFY 181

Query: 198 IGTLSVAVGSSRALYGEIKEVWLDGAKGKDEKDMEYFFDSWFSLIHQLQPGAVIFSDAGP 257
           +G ++  +      YGEIKEVWLDGAKGKDEKDMEYFFDSWFSLIHQLQPGAVIFSDAGP
Sbjct: 182 VGQMTELL----TRYGEIKEVWLDGAKGKDEKDMEYFFDSWFSLIHQLQPGAVIFSDAGP 241

Query: 258 DCRWIGDEAGVAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGHDWVPAECDVSIRPGWFW 317
           DCRWIGDEAGVAKPTCWSLFNCSA KIGGTDSRYSGEGDPFGHDWVPAECDVSIRPGWFW
Sbjct: 242 DCRWIGDEAGVAKPTCWSLFNCSAVKIGGTDSRYSGEGDPFGHDWVPAECDVSIRPGWFW 301

Query: 318 HPLEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDIQVLQEFTKIRDSIFSHN 377
           HP EVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSG ISAEDIQVLQEFTKIRDSIFSHN
Sbjct: 302 HPSEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGSISAEDIQVLQEFTKIRDSIFSHN 361

Query: 378 LAEKALVDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPTWSLYLNLQEFISFNVL 437
           LAE AL+DASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPTWSLYLNLQEFISFNVL
Sbjct: 362 LAENALLDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPTWSLYLNLQEFISFNVL 421

Query: 438 HIQEPIHMGQRIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKFPTVESQFLRLVIEKSRA 497
           HIQEPIHMGQRI EFHFD+LN+EGVWRTVVRGSTVGYRRILKFPTVESQFLRLVIEKSRA
Sbjct: 422 HIQEPIHMGQRIAEFHFDILNEEGVWRTVVRGSTVGYRRILKFPTVESQFLRLVIEKSRA 481

Query: 498 DPLVSYLGIHVDNFSHLSSHLDKTLGAGINGSNIRRQITLNNSQISAV 519
           DPLVSYLGIHVDNFSHLSS  DKTLGAGINGS+I RQITLNNSQISAV
Sbjct: 482 DPLVSYLGIHVDNFSHLSSRCDKTLGAGINGSDILRQITLNNSQISAV 525

[Close]

BLAST of Carg03213 vs. ExPASy TrEMBL
Match: A0A0A0KAV7 (Alpha-L-fucosidase OS=Cucumis sativus OX=3659 GN=Csa_6G147630 PE=3 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 4.0e-242
Identity = 426/544 (78.31%), Postives = 457/544 (84.01%), Query Frame = 0


Query: 3   KKQAIRFFGYCNNIPKPSSALNSISQTQISIVFLLSLLLQPISSSSSS--ISSTPPLPIL 62
           K   I FF  C N PKP S LN IS    S+ FLLSL LQP+SSSSSS  I++ PPLPIL
Sbjct: 2   KNHNIIFFTNC-NFPKPFSLLNLIS--SFSLFFLLSLFLQPVSSSSSSSLINNPPPLPIL 61

Query: 63  PIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHVDPSAFNPTNLDAAQWVRVAKDAGF 122
           PIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHV PS FNP NLDAAQWVRVAKDAGF
Sbjct: 62  PIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTGHVHPSVFNPINLDAAQWVRVAKDAGF 121

Query: 123 SRLILTAKHHDGFCLWPSEYTNYSVRSSPWR------------MAKE------MWLGNWP 182
           SR+ILTAKHHDGFCLWPSEYTNYSV SSPWR             AKE      ++L  W 
Sbjct: 122 SRIILTAKHHDGFCLWPSEYTNYSVSSSPWRNGKGDVVGELAKAAKEAGLGLGLYLSPWD 181

Query: 183 R--------LQRSRTWIGTLSVAVGSSRALYGEIKEVWLDGAKGKDEKDMEYFFDSWFSL 242
           R        L+ +  ++G ++  +      YGEIKEVWLDGAKG+ EKDMEYFFDSWFSL
Sbjct: 182 RHESCYGKTLEYNEFYLGQMTELL----TRYGEIKEVWLDGAKGEGEKDMEYFFDSWFSL 241

Query: 243 IHQLQPGAVIFSDAGPDCRWIGDEAGVAKPTCWSLFNCSAAKIGGTDSRYSGEGDPFGHD 302
           IHQLQPGAVIFSDAGPDCRWIGDEAGVAKPTCWSLFN S+AKIGGTDSRYSGEGDP+GHD
Sbjct: 242 IHQLQPGAVIFSDAGPDCRWIGDEAGVAKPTCWSLFNSSSAKIGGTDSRYSGEGDPYGHD 301

Query: 303 WVPAECDVSIRPGWFWHPLEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGLISAEDIQ 362
           W+PAECDVSIRPGWFWHP EVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGLIS ED+Q
Sbjct: 302 WIPAECDVSIRPGWFWHPSEVPKSARTLLDIYYKSAGRNCLLLLNVPPNSSGLISDEDVQ 361

Query: 363 VLQEFTKIRDSIFSHNLAEKALVDASSTRGNGEDDRFIPSNVLKEGIYTYWAPAQNHQPT 422
           VLQEFT IRDSIFS+NLAE ALVD SSTRG+ ED RF P+NVLKEGIYTYWAPA+N QPT
Sbjct: 362 VLQEFTMIRDSIFSYNLAENALVDGSSTRGDDEDVRFTPANVLKEGIYTYWAPAEN-QPT 421

Query: 423 WSLYLNLQEFISFNVLHIQEPIHMGQRIVEFHFDVLNDEGVWRTVVRGSTVGYRRILKFP 482
           W+LYLNLQ+FISFNVL IQEPIHMGQRI+EFHFD+LNDEGVW TVV G+TVGYRRIL+FP
Sbjct: 422 WALYLNLQDFISFNVLLIQEPIHMGQRIIEFHFDILNDEGVWSTVVTGTTVGYRRILRFP 481

Query: 483 TVESQFLRLVIEKSRADPLVSYLGIHVDNFSHLSSHLDKTLGAGINGSNIRRQITLNNSQ 519
            VESQFLRLVIEKSRADPLVSYLGIH+DNFSHL SHLDKT    INGS IRRQITLNNS+
Sbjct: 482 AVESQFLRLVIEKSRADPLVSYLGIHLDNFSHLDSHLDKT---SINGSEIRRQITLNNSE 534

[Close]

BLAST of Carg03213 vs. TAIR 10
Match: AT2G28100.1 (alpha-L-fucosidase 1 )

HSP 1 Score: 586.6 bits (1511), Expect = 1.9e-167
Identity = 291/487 (59.75%), Postives = 359/487 (73.72%), Query Frame = 0


Query: 35  FLLSLLLQPISSSSSSISSTPPLPILPIPSASQIQWQLGNMALFLHFGPNTFTDSEWGTG 94
           F  S+L     S+SSS+    P PILP+PS+ Q+QWQLG+MA+FLHFGPNTFTDSEWGTG
Sbjct: 10  FFFSILSLSQISNSSSLLKPHPCPILPLPSSQQLQWQLGSMAMFLHFGPNTFTDSEWGTG 69

Query: 95  HVDPSAFNPTNLDAAQWVRVAKDAGFSRLILTAKHHDGFCLWPSEYTNYSVRSSPWR--- 154
             +PS FNPT+L+A+QWV++AKD+GFSR+ILTAKHHDGFCLWPSEYT+YSV+SS WR   
Sbjct: 70  KANPSIFNPTHLNASQWVQIAKDSGFSRVILTAKHHDGFCLWPSEYTDYSVKSSQWRNGA 129

Query: 155 ---------MAKE------MWLGNWPR--------LQRSRTWIGTLSVAVGSSRALYGEI 214
                     AKE      ++L  W R        L+ +  ++  ++  +      YGEI
Sbjct: 130 GDVVAELASAAKEAGIGLGLYLSPWDRHEQCYGKTLEYNEFYLSQMTELL----TKYGEI 189

Query: 215 KEVWLDGAKGKDEKDMEYFFDSWFSLIHQLQPGAVIFSDAGPDCRWIGDEAGVAKPTCWS 274
           KEVWLDGAKG  EKDMEYFFD+WFSLIHQLQP AVIFSDAGPD RWIGDEAG+A  TCWS
Sbjct: 190 KEVWLDGAKGDGEKDMEYFFDTWFSLIHQLQPKAVIFSDAGPDVRWIGDEAGLAGSTCWS 249

Query: 275 LFNCSAAKIGGTDSRYSGEGDPFGHDWVPAECDVSIRPGWFWHPLEVPKSARTLLDIYYK 334
           LFN + AKIG T+  YS EGD +G DWVPAECDVSIRPGWFWH  E PK A  LLDIYY 
Sbjct: 250 LFNRTNAKIGDTEPSYSQEGDGYGQDWVPAECDVSIRPGWFWHASESPKPAVQLLDIYYN 309

Query: 335 SAGRNCLLLLNVPPNSSGLISAEDIQVLQEFTKIRDSIFSHNLAEKALVDASSTRGNGED 394
           S GRNCL LLNVPPNSSGLIS +DI+VL+EF+++++SIFS+NLA KA V++SS RG+ + 
Sbjct: 310 SVGRNCLFLLNVPPNSSGLISEQDIKVLEEFSEMKNSIFSNNLARKAFVNSSSIRGD-QS 369

Query: 395 DRFIPSNVLKEGIYTYWAPAQNHQPTWSLYLNLQEFISFNVLHIQEPIHMGQRIVEFHFD 454
            +F P NVL+EG+  YWAP +N Q  W LYL  ++ +SFNVL I+EPIHMGQRI  FH +
Sbjct: 370 SQFGPKNVLEEGLDKYWAPEEN-QNEWVLYLEFKDLVSFNVLEIREPIHMGQRIASFHLE 429

Query: 455 V-LNDEGVWRTVVRGSTVGYRRILKF-PTVESQFLRLVIEKSRADPLVSYLGIHVDNFSH 494
                 G W  VV G+TVG +R+L+F   VES+ L+LV++K+R DPL+SYLG+++D FS 
Sbjct: 430 TRKTGSGEWERVVSGTTVGNKRLLRFLNVVESRSLKLVVDKARTDPLISYLGLYMDKFSG 489

The following BLAST results are available for this feature:

BLAST of Carg03213 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7010653.1	0.0e+00	100.00	Alpha-L-fucosidase 1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6570812.1	7.2e-286	90.26	Alpha-L-fucosidase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022944007.1	1.8e-284	89.71	alpha-L-fucosidase 1 isoform X1 [Cucurbita moschata]	[more]
XP_022985567.1	6.8e-276	87.52	alpha-L-fucosidase 1 isoform X1 [Cucurbita maxima]	[more]
XP_022944008.1	4.5e-272	88.99	alpha-L-fucosidase 1 isoform X2 [Cucurbita moschata]	[more]

BLAST of Carg03213 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GW72	2.7e-166	59.75	Alpha-L-fucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=FUC1 PE=1 SV=2	[more]
Q7XUR3	2.8e-155	54.18	Putative alpha-L-fucosidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0...	[more]
Q9VTJ4	2.0e-04	46.34	Putative alpha-L-fucosidase OS=Drosophila melanogaster OX=7227 GN=Fuca PE=2 SV=2	[more]

BLAST of Carg03213 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FUL0	8.6e-285	89.71	Alpha-L-fucosidase OS=Cucurbita moschata OX=3662 GN=LOC111448567 PE=3 SV=1	[more]
A0A6J1J578	3.3e-276	87.52	Alpha-L-fucosidase OS=Cucurbita maxima OX=3661 GN=LOC111483599 PE=3 SV=1	[more]
A0A6J1FXK9	2.2e-272	88.99	Alpha-L-fucosidase OS=Cucurbita moschata OX=3662 GN=LOC111448567 PE=3 SV=1	[more]
A0A6J1JDZ9	1.3e-264	86.74	Alpha-L-fucosidase OS=Cucurbita maxima OX=3661 GN=LOC111483599 PE=3 SV=1	[more]
A0A0A0KAV7	4.0e-242	78.31	Alpha-L-fucosidase OS=Cucumis sativus OX=3659 GN=Csa_6G147630 PE=3 SV=1	[more]

BLAST of Carg03213 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28100.1	1.9e-167	59.75	alpha-L-fucosidase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000933	Glycoside hydrolase, family 29	SMART	SM00812	alpha_l_fucos	coord: 58..376 e-value: 3.0E-7 score: -79.7
IPR000933	Glycoside hydrolase, family 29	PFAM	PF01120	Alpha_L_fucos	coord: 99..147 e-value: 1.3E-12 score: 47.8
IPR000933	Glycoside hydrolase, family 29	PANTHER	PTHR10030	ALPHA-L-FUCOSIDASE	coord: 30..154 coord: 184..509
None	No IPR available	GENE3D	2.60.120.260		coord: 362..480 e-value: 1.4E-24 score: 88.3
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 56..160 e-value: 1.9E-42 score: 147.6 coord: 183..352 e-value: 5.2E-47 score: 162.7
None	No IPR available	PANTHER	PTHR10030:SF27	ALPHA-L-FUCOSIDASE 1	coord: 30..154 coord: 184..509
IPR008979	Galactose-binding-like domain superfamily	SUPERFAMILY	49785	Galactose-binding domain-like	coord: 349..471
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 58..343

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg03213-RA	Carg03213-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006004	fucose metabolic process
biological_process	GO:0016139	glycoside catabolic process
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0005764	lysosome
molecular_function	GO:0004560	alpha-L-fucosidase activity