Carg03188 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg03188
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	phospholipid:diacylglycerol acyltransferase
Location	Carg_Chr20: 2663680 .. 2664650 (-)
RNA-Seq Expression	Carg03188
Synteny	Carg03188

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCAGGTAGCTCCAATGATTCGAGCTTCTCTTGTAGCGACGGATGGACTGAATATGATGAGATGAGTAGGGAAAGTCTTCGGAAAGTCTCCGAGAATAAAGCTTACACAGCAAAAACTGTTTTTGATCTACTCCGAGTCGTGGCTCCAAAAATGATGCAGAGGATGGATAGTCACTTCTCTCATGGGATTGCTGAAGATCTTGATGATCCAAAATATGCTCATTACAAGTACTGGTCAAATCCACTAGAGACAAAGTAAGTAGCTGTGTAGTTTTTTAGAATTTTCTGATTTCTTTAGAAGATAATCTTCCACATGAAGTACTGAATTGAATCAACGCTTGGCATATGAGCTGATAATCTATCTAATAACTATCTAACATCAAGAACATGGTTTTGGAGATGGTATTATTAGCTAATTTCAAATACAATCAAACAACTTCCCTCAATCCCATGAGACTTGATACATTTTGCAAAATTTGTTTCAGACTACCAGATGCCCCAGATATGGAGATATACTGCTTATACGGAGTGGGAATCCCCACAGAAAGATCATATATCTATGACTCATCTTATGATAAGTGCAAGAGCATTCCATTCAGGATTGATCTCTCAATCCATGGAGAAGGGAGTGGCTGTCTAAGAAGTGGGATGTATTTTGTCGATGGCGACGACAGTGTACCAGTGGTGAGCTCTGGTTTCATGTGTGCGAAAGGGTGGCGAAACAAAACACGATTCAATCCATCAGGCAGCCCTACATACGTAAGAGAATACAGGCACAAGGCTCCTGCTAGTCTGCTAGAAGGAAGAGGAATTGAAAGTAGTGCTCACGTTGACATAATGGGCAATGCTAAACTAATTGAAGATATCTTGAGGGTTGCTGCTGGAGCCACTGGAGCAGAGATTGGTGGTGATAGGATTTACTCAGATATATTGACACTATCAGAGAGAATAAATCTTAAGCTGTGA

mRNA sequence

ATGGATTCAGGTAGCTCCAATGATTCGAGCTTCTCTTGTAGCGACGGATGGACTGAATATGATGAGATGAGTAGGGAAAGTCTTCGGAAAGTCTCCGAGAATAAAGCTTACACAGCAAAAACTGTTTTTGATCTACTCCGAGTCGTGGCTCCAAAAATGATGCAGAGGATGGATAGTCACTTCTCTCATGGGATTGCTGAAGATCTTGATGATCCAAAATATGCTCATTACAAGTACTGGTCAAATCCACTAGAGACAAAACTACCAGATGCCCCAGATATGGAGATATACTGCTTATACGGAGTGGGAATCCCCACAGAAAGATCATATATCTATGACTCATCTTATGATAAGTGCAAGAGCATTCCATTCAGGATTGATCTCTCAATCCATGGAGAAGGGAGTGGCTGTCTAAGAAGTGGGATGTATTTTGTCGATGGCGACGACAGTGTACCAGTGGTGAGCTCTGGTTTCATGTGTGCGAAAGGGTGGCGAAACAAAACACGATTCAATCCATCAGGCAGCCCTACATACGTAAGAGAATACAGGCACAAGGCTCCTGCTAGTCTGCTAGAAGGAAGAGGAATTGAAAGTAGTGCTCACGTTGACATAATGGGCAATGCTAAACTAATTGAAGATATCTTGAGGGTTGCTGCTGGAGCCACTGGAGCAGAGATTGGTGGTGATAGGATTTACTCAGATATATTGACACTATCAGAGAGAATAAATCTTAAGCTGTGA

Coding sequence (CDS)

ATGGATTCAGGTAGCTCCAATGATTCGAGCTTCTCTTGTAGCGACGGATGGACTGAATATGATGAGATGAGTAGGGAAAGTCTTCGGAAAGTCTCCGAGAATAAAGCTTACACAGCAAAAACTGTTTTTGATCTACTCCGAGTCGTGGCTCCAAAAATGATGCAGAGGATGGATAGTCACTTCTCTCATGGGATTGCTGAAGATCTTGATGATCCAAAATATGCTCATTACAAGTACTGGTCAAATCCACTAGAGACAAAACTACCAGATGCCCCAGATATGGAGATATACTGCTTATACGGAGTGGGAATCCCCACAGAAAGATCATATATCTATGACTCATCTTATGATAAGTGCAAGAGCATTCCATTCAGGATTGATCTCTCAATCCATGGAGAAGGGAGTGGCTGTCTAAGAAGTGGGATGTATTTTGTCGATGGCGACGACAGTGTACCAGTGGTGAGCTCTGGTTTCATGTGTGCGAAAGGGTGGCGAAACAAAACACGATTCAATCCATCAGGCAGCCCTACATACGTAAGAGAATACAGGCACAAGGCTCCTGCTAGTCTGCTAGAAGGAAGAGGAATTGAAAGTAGTGCTCACGTTGACATAATGGGCAATGCTAAACTAATTGAAGATATCTTGAGGGTTGCTGCTGGAGCCACTGGAGCAGAGATTGGTGGTGATAGGATTTACTCAGATATATTGACACTATCAGAGAGAATAAATCTTAAGCTGTGA

Protein sequence

MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSERINLKL

Homology

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BLAST of Carg03188 vs. NCBI nr
Match: KAG7010678.1 (putative phospholipid:diacylglycerol acyltransferase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 506.1 bits (1302), Expect = 1.7e-139
Identity = 246/246 (100.00%), Postives = 246/246 (100.00%), Query Frame = 0


Query: 1   MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH 60
           MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH
Sbjct: 1   MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH 60

Query: 61  FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK 120
           FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK
Sbjct: 61  FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK 120

Query: 121 SIPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 180
           SIPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR
Sbjct: 121 SIPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 180

Query: 181 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE 240
           EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE
Sbjct: 181 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE 240

Query: 241 RINLKL 247
           RINLKL
Sbjct: 241 RINLKL 246

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BLAST of Carg03188 vs. NCBI nr
Match: KAG6570836.1 (putative phospholipid:diacylglycerol acyltransferase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 502.7 bits (1293), Expect = 1.8e-138
Identity = 245/246 (99.59%), Postives = 245/246 (99.59%), Query Frame = 0


Query: 1   MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH 60
           MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH
Sbjct: 449 MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH 508

Query: 61  FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK 120
           FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK
Sbjct: 509 FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK 568

Query: 121 SIPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 180
           SIPFRIDLSI GEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR
Sbjct: 569 SIPFRIDLSIDGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 628

Query: 181 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE 240
           EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE
Sbjct: 629 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE 688

Query: 241 RINLKL 247
           RINLKL
Sbjct: 689 RINLKL 694

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BLAST of Carg03188 vs. NCBI nr
Match: XP_023512597.1 (putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 501.1 bits (1289), Expect = 5.3e-138
Identity = 244/246 (99.19%), Postives = 245/246 (99.59%), Query Frame = 0


Query: 1   MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH 60
           MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH
Sbjct: 449 MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH 508

Query: 61  FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK 120
           FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK
Sbjct: 509 FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK 568

Query: 121 SIPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 180
           SIPFRIDLSI GEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR
Sbjct: 569 SIPFRIDLSIDGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 628

Query: 181 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE 240
           EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGG+RIYSDILTLSE
Sbjct: 629 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGERIYSDILTLSE 688

Query: 241 RINLKL 247
           RINLKL
Sbjct: 689 RINLKL 694

[Close]

BLAST of Carg03188 vs. NCBI nr
Match: XP_022986276.1 (putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita maxima])

HSP 1 Score: 500.4 bits (1287), Expect = 9.1e-138
Identity = 243/246 (98.78%), Postives = 244/246 (99.19%), Query Frame = 0


Query: 1   MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH 60
           MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH
Sbjct: 449 MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH 508

Query: 61  FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK 120
           FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK
Sbjct: 509 FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK 568

Query: 121 SIPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 180
           SIPFRIDLS+ GEGSGCLRSGMYFVDGDD VPVVSSGFMCAKGWRNKTRFNPSGSPTYVR
Sbjct: 569 SIPFRIDLSVDGEGSGCLRSGMYFVDGDDCVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 628

Query: 181 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE 240
           EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE
Sbjct: 629 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE 688

Query: 241 RINLKL 247
           RINLKL
Sbjct: 689 RINLKL 694

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BLAST of Carg03188 vs. NCBI nr
Match: XP_022944139.1 (putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita moschata])

HSP 1 Score: 498.0 bits (1281), Expect = 4.5e-137
Identity = 242/246 (98.37%), Postives = 243/246 (98.78%), Query Frame = 0


Query: 1   MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH 60
           MDSGSSND+SFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH
Sbjct: 449 MDSGSSNDTSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH 508

Query: 61  FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK 120
           FSHGIAEDLDDPKY HYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK
Sbjct: 509 FSHGIAEDLDDPKYGHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK 568

Query: 121 SIPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 180
           SIPFRIDLSI GEGSGCLRSGMYFVDGDD VPVVSSGFMCAKGWRNKTRFNPSGSPTYVR
Sbjct: 569 SIPFRIDLSIDGEGSGCLRSGMYFVDGDDCVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 628

Query: 181 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE 240
           EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE
Sbjct: 629 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE 688

Query: 241 RINLKL 247
           RINLKL
Sbjct: 689 RINLKL 694

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BLAST of Carg03188 vs. ExPASy Swiss-Prot
Match: Q9FYC7 (Putative phospholipid:diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=PDAT2 PE=3 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 1.0e-99
Identity = 176/246 (71.54%), Postives = 209/246 (84.96%), Query Frame = 0


Query: 4   GSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHFSH 63
           G+SNDS+ SC + W+EY+EMSRES+ KV+EN AYTA TV DLLR +APKMM+R ++HFSH
Sbjct: 422 GNSNDST-SCGEFWSEYNEMSRESIVKVAENTAYTATTVLDLLRFIAPKMMRRAEAHFSH 481

Query: 64  GIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYD--SSYDKCK- 123
           GIA+DLDDPKY HYKYWSNPLETKLP+AP+ME+YCLYGVGIPTERSYIY   +S  KCK 
Sbjct: 482 GIADDLDDPKYGHYKYWSNPLETKLPEAPEMEMYCLYGVGIPTERSYIYKLATSSGKCKS 541

Query: 124 SIPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 183
           SIPFRID S+ G+   CL+ G  F DGD+SVPV+S+GFMCAKGWR KTRFNPSG  T++R
Sbjct: 542 SIPFRIDGSLDGD-DVCLKGGTRFADGDESVPVISAGFMCAKGWRGKTRFNPSGMDTFLR 601

Query: 184 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE 243
           EY+HK P SLLE RG ES AHVDIMGN  LIED+LR+AAGA+G EIGGDRIYSD++ +SE
Sbjct: 602 EYKHKPPGSLLESRGTESGAHVDIMGNVGLIEDVLRIAAGASGQEIGGDRIYSDVMRMSE 661

Query: 244 RINLKL 247
           RI++KL
Sbjct: 662 RISIKL 665

[Close]

BLAST of Carg03188 vs. ExPASy Swiss-Prot
Match: Q9FNA9 (Phospholipid:diacylglycerol acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=PDAT1 PE=2 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 1.1e-80
Identity = 144/236 (61.02%), Postives = 177/236 (75.00%), Query Frame = 0


Query: 12  SCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDD 71
           +C D WTEY +M    ++ ++E K YTA    DLL  VAPKMM R  +HFS+GIA+DLDD
Sbjct: 436 TCRDVWTEYHDMGIAGIKAIAEYKVYTAGEAIDLLHYVAPKMMARGAAHFSYGIADDLDD 495

Query: 72  PKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIH 131
            KY   KYWSNPLETKLP+AP+MEIY LYGVGIPTER+Y+Y  +      IPF+I  S H
Sbjct: 496 TKYQDPKYWSNPLETKLPNAPEMEIYSLYGVGIPTERAYVYKLNQSPDSCIPFQIFTSAH 555

Query: 132 GEG-SGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASL 191
            E    CL++G+Y VDGD++VPV+S+G+MCAK WR KTRFNPSG  TY+REY H  PA+L
Sbjct: 556 EEDEDSCLKAGVYNVDGDETVPVLSAGYMCAKAWRGKTRFNPSGIKTYIREYNHSPPANL 615

Query: 192 LEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSERINLKL 247
           LEGRG +S AHVDIMGN  LIEDI+RVAAG  G++IG D+++S I   SERI+LKL
Sbjct: 616 LEGRGTQSGAHVDIMGNFALIEDIMRVAAGGNGSDIGHDQVHSGIFEWSERIDLKL 671

[Close]

BLAST of Carg03188 vs. ExPASy Swiss-Prot
Match: P40345 (Phospholipid:diacylglycerol acyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=LRO1 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 4.1e-24
Identity = 69/219 (31.51%), Postives = 124/219 (56.62%), Query Frame = 0


Query: 5   SSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQ-RMDSHFSH 64
           SS D+  + +D +  +    R +    + NK  T K   ++   ++P+ +Q R+   +S 
Sbjct: 432 SSEDALNNNTDTYGNFIRFERNT--SDAFNKNLTMKDAINMTLSISPEWLQRRVHEQYSF 491

Query: 65  GIAEDLDD--PKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKS 124
           G +++ ++      H+K+WSNP+E  LP+AP M+IYC+YGV  PTER+Y+Y    D    
Sbjct: 492 GYSKNEEELRKNELHHKHWSNPMEVPLPEAPHMKIYCIYGVNNPTERAYVYKEEDDSS-- 551

Query: 125 IPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVRE 184
               ++L+I  E     +  ++  +GD +VP+V+   MC K  +  + +NP+G    + E
Sbjct: 552 ---ALNLTIDYES----KQPVFLTEGDGTVPLVAHS-MCHKWAQGASPYNPAGINVTIVE 611

Query: 185 YRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAG 221
            +H+     + G G +S+ HVDI+G+A+L + IL++A+G
Sbjct: 612 MKHQPDRFDIRG-GAKSAEHVDILGSAELNDYILKIASG 637

[Close]

BLAST of Carg03188 vs. ExPASy Swiss-Prot
Match: O94680 (Phospholipid:diacylglycerol acyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=plh1 PE=1 SV=2)

HSP 1 Score: 112.5 bits (280), Expect = 7.0e-24
Identity = 74/189 (39.15%), Postives = 114/189 (60.32%), Query Frame = 0


Query: 60  HFSHGIA----EDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSS 119
           ++SHG+A    E L + +      W NPLET LP APDM+IYC++GVG PTER Y Y ++
Sbjct: 451 NYSHGLAWTEKEVLKNNEMP--SKWINPLETSLPYAPDMKIYCVHGVGKPTERGYYYTNN 510

Query: 120 YDKCKSIPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGS 179
            +     P  ID S++ +G+  + +G+   DGD ++P+++ G +C K W+ K RFNP+ +
Sbjct: 511 PE---GQPV-IDSSVN-DGTK-VENGIVMDDGDGTLPILALGLVCNKVWQTK-RFNPANT 570

Query: 180 PTYVREYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDI 239
                E +H+  A  L G G  S+ HVDI+G+++L E IL+V++G    +   +R  SDI
Sbjct: 571 SITNYEIKHEPAAFDLRG-GPRSAEHVDILGHSELNEIILKVSSG--HGDSVPNRYISDI 627

Query: 240 LTLSERINL 245
             +   INL
Sbjct: 631 QEIINEINL 627

[Close]

BLAST of Carg03188 vs. ExPASy Swiss-Prot
Match: Q08758 (Phosphatidylcholine-sterol acyltransferase OS=Papio anubis OX=9555 GN=LCAT PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.1e-05
Identity = 42/141 (29.79%), Postives = 64/141 (45.39%), Query Frame = 0


Query: 81  SNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIHGEGSGCLRS 140
           S  L   LP AP +E+YCLYGVG+PT R+YIYD  +      P+   + +          
Sbjct: 321 SRDLLAGLP-APGVEVYCLYGVGLPTPRTYIYDHGF------PYTDPVDV---------- 380

Query: 141 GMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGIESSA 200
              + DGDD+V   S+  +C   W+                 R   P  LL  RGI+   
Sbjct: 381 --LYEDGDDTVATRSTE-LCGL-WQG----------------RQPQPVHLLPLRGIQ--- 421

Query: 201 HVDIMGNAKLIEDILRVAAGA 222
           H++++ + + +E I  +  GA
Sbjct: 441 HLNMVFSNQTLEHINAILLGA 421

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BLAST of Carg03188 vs. ExPASy TrEMBL
Match: A0A6J1JAN3 (putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucurbita maxima OX=3661 GN=LOC111484072 PE=4 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 4.4e-138
Identity = 243/246 (98.78%), Postives = 244/246 (99.19%), Query Frame = 0


Query: 1   MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH 60
           MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH
Sbjct: 449 MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH 508

Query: 61  FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK 120
           FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK
Sbjct: 509 FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK 568

Query: 121 SIPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 180
           SIPFRIDLS+ GEGSGCLRSGMYFVDGDD VPVVSSGFMCAKGWRNKTRFNPSGSPTYVR
Sbjct: 569 SIPFRIDLSVDGEGSGCLRSGMYFVDGDDCVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 628

Query: 181 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE 240
           EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE
Sbjct: 629 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE 688

Query: 241 RINLKL 247
           RINLKL
Sbjct: 689 RINLKL 694

[Close]

BLAST of Carg03188 vs. ExPASy TrEMBL
Match: A0A6J1FW53 (putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucurbita moschata OX=3662 GN=LOC111448684 PE=4 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 2.2e-137
Identity = 242/246 (98.37%), Postives = 243/246 (98.78%), Query Frame = 0


Query: 1   MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH 60
           MDSGSSND+SFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH
Sbjct: 449 MDSGSSNDTSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH 508

Query: 61  FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK 120
           FSHGIAEDLDDPKY HYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK
Sbjct: 509 FSHGIAEDLDDPKYGHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK 568

Query: 121 SIPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 180
           SIPFRIDLSI GEGSGCLRSGMYFVDGDD VPVVSSGFMCAKGWRNKTRFNPSGSPTYVR
Sbjct: 569 SIPFRIDLSIDGEGSGCLRSGMYFVDGDDCVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 628

Query: 181 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE 240
           EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE
Sbjct: 629 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE 688

Query: 241 RINLKL 247
           RINLKL
Sbjct: 689 RINLKL 694

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BLAST of Carg03188 vs. ExPASy TrEMBL
Match: A0A5A7SR19 (Putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold26G00090 PE=4 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 7.1e-128
Identity = 226/245 (92.24%), Postives = 235/245 (95.92%), Query Frame = 0


Query: 2   DSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHF 61
           +SG SNDSSFSCSD WTEYDEMSR+S+RKVSENKAYT KTVFDLLRVVAPKMMQRMDSHF
Sbjct: 449 NSGGSNDSSFSCSDVWTEYDEMSRDSIRKVSENKAYTVKTVFDLLRVVAPKMMQRMDSHF 508

Query: 62  SHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKS 121
           SHGIAEDLDDPKYAH+KYWSNPLETKLPDAP+MEIYCLYGVGIPTERSYIYDSSYDKCKS
Sbjct: 509 SHGIAEDLDDPKYAHHKYWSNPLETKLPDAPNMEIYCLYGVGIPTERSYIYDSSYDKCKS 568

Query: 122 IPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVRE 181
           IPFRIDLSI G+GSGCLRSGMYFVDGDDSVP+VSSGFMCAKGWRNKTRFNPSGSPTYVRE
Sbjct: 569 IPFRIDLSIEGKGSGCLRSGMYFVDGDDSVPIVSSGFMCAKGWRNKTRFNPSGSPTYVRE 628

Query: 182 YRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSER 241
           YRHKAPASLLEGRG+ESSAHVDIMGN  LIEDILRVAA   G EIGGD+IYSDILTLSER
Sbjct: 629 YRHKAPASLLEGRGVESSAHVDIMGNVNLIEDILRVAA---GEEIGGDKIYSDILTLSER 688

Query: 242 INLKL 247
           INLKL
Sbjct: 689 INLKL 690

[Close]

BLAST of Carg03188 vs. ExPASy TrEMBL
Match: A0A1S3C9K8 (putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucumis melo OX=3656 GN=LOC103497986 PE=4 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 7.1e-128
Identity = 226/245 (92.24%), Postives = 235/245 (95.92%), Query Frame = 0


Query: 2   DSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHF 61
           +SG SNDSSFSCSD WTEYDEMSR+S+RKVSENKAYT KTVFDLLRVVAPKMMQRMDSHF
Sbjct: 562 NSGGSNDSSFSCSDVWTEYDEMSRDSIRKVSENKAYTVKTVFDLLRVVAPKMMQRMDSHF 621

Query: 62  SHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKS 121
           SHGIAEDLDDPKYAH+KYWSNPLETKLPDAP+MEIYCLYGVGIPTERSYIYDSSYDKCKS
Sbjct: 622 SHGIAEDLDDPKYAHHKYWSNPLETKLPDAPNMEIYCLYGVGIPTERSYIYDSSYDKCKS 681

Query: 122 IPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVRE 181
           IPFRIDLSI G+GSGCLRSGMYFVDGDDSVP+VSSGFMCAKGWRNKTRFNPSGSPTYVRE
Sbjct: 682 IPFRIDLSIEGKGSGCLRSGMYFVDGDDSVPIVSSGFMCAKGWRNKTRFNPSGSPTYVRE 741

Query: 182 YRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSER 241
           YRHKAPASLLEGRG+ESSAHVDIMGN  LIEDILRVAA   G EIGGD+IYSDILTLSER
Sbjct: 742 YRHKAPASLLEGRGVESSAHVDIMGNVNLIEDILRVAA---GEEIGGDKIYSDILTLSER 801

Query: 242 INLKL 247
           INLKL
Sbjct: 802 INLKL 803

[Close]

BLAST of Carg03188 vs. ExPASy TrEMBL
Match: A0A0A0KGM0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G149960 PE=4 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 9.2e-128
Identity = 226/245 (92.24%), Postives = 235/245 (95.92%), Query Frame = 0


Query: 2   DSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHF 61
           +SG SNDSSFSCSD WTEYDEMSRES+RKVSENKAYT +TVFDLLR+VAPKMMQRMDSHF
Sbjct: 449 NSGGSNDSSFSCSDVWTEYDEMSRESIRKVSENKAYTVETVFDLLRIVAPKMMQRMDSHF 508

Query: 62  SHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKS 121
           SHGIAEDLDDPKYAH+KYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKS
Sbjct: 509 SHGIAEDLDDPKYAHHKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKS 568

Query: 122 IPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVRE 181
           IPFRIDLSI G+GSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPT+VRE
Sbjct: 569 IPFRIDLSIEGKGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTHVRE 628

Query: 182 YRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSER 241
           YRHKAPASLLEGRG+ESSAHVDIMGN  LIEDILRVAA   G EIGGD+IYSDILTLSER
Sbjct: 629 YRHKAPASLLEGRGVESSAHVDIMGNVNLIEDILRVAA---GEEIGGDKIYSDILTLSER 688

Query: 242 INLKL 247
           INLKL
Sbjct: 689 INLKL 690

[Close]

BLAST of Carg03188 vs. TAIR 10
Match: AT3G44830.1 (Lecithin:cholesterol acyltransferase family protein )

HSP 1 Score: 364.4 bits (934), Expect = 7.3e-101
Identity = 176/246 (71.54%), Postives = 209/246 (84.96%), Query Frame = 0


Query: 4   GSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHFSH 63
           G+SNDS+ SC + W+EY+EMSRES+ KV+EN AYTA TV DLLR +APKMM+R ++HFSH
Sbjct: 422 GNSNDST-SCGEFWSEYNEMSRESIVKVAENTAYTATTVLDLLRFIAPKMMRRAEAHFSH 481

Query: 64  GIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYD--SSYDKCK- 123
           GIA+DLDDPKY HYKYWSNPLETKLP+AP+ME+YCLYGVGIPTERSYIY   +S  KCK 
Sbjct: 482 GIADDLDDPKYGHYKYWSNPLETKLPEAPEMEMYCLYGVGIPTERSYIYKLATSSGKCKS 541

Query: 124 SIPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 183
           SIPFRID S+ G+   CL+ G  F DGD+SVPV+S+GFMCAKGWR KTRFNPSG  T++R
Sbjct: 542 SIPFRIDGSLDGD-DVCLKGGTRFADGDESVPVISAGFMCAKGWRGKTRFNPSGMDTFLR 601

Query: 184 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE 243
           EY+HK P SLLE RG ES AHVDIMGN  LIED+LR+AAGA+G EIGGDRIYSD++ +SE
Sbjct: 602 EYKHKPPGSLLESRGTESGAHVDIMGNVGLIEDVLRIAAGASGQEIGGDRIYSDVMRMSE 661

Query: 244 RINLKL 247
           RI++KL
Sbjct: 662 RISIKL 665

[Close]

BLAST of Carg03188 vs. TAIR 10
Match: AT5G13640.1 (phospholipid:diacylglycerol acyltransferase )

HSP 1 Score: 301.2 bits (770), Expect = 7.6e-82
Identity = 144/236 (61.02%), Postives = 177/236 (75.00%), Query Frame = 0


Query: 12  SCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDD 71
           +C D WTEY +M    ++ ++E K YTA    DLL  VAPKMM R  +HFS+GIA+DLDD
Sbjct: 436 TCRDVWTEYHDMGIAGIKAIAEYKVYTAGEAIDLLHYVAPKMMARGAAHFSYGIADDLDD 495

Query: 72  PKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIH 131
            KY   KYWSNPLETKLP+AP+MEIY LYGVGIPTER+Y+Y  +      IPF+I  S H
Sbjct: 496 TKYQDPKYWSNPLETKLPNAPEMEIYSLYGVGIPTERAYVYKLNQSPDSCIPFQIFTSAH 555

Query: 132 GEG-SGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASL 191
            E    CL++G+Y VDGD++VPV+S+G+MCAK WR KTRFNPSG  TY+REY H  PA+L
Sbjct: 556 EEDEDSCLKAGVYNVDGDETVPVLSAGYMCAKAWRGKTRFNPSGIKTYIREYNHSPPANL 615

Query: 192 LEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSERINLKL 247
           LEGRG +S AHVDIMGN  LIEDI+RVAAG  G++IG D+++S I   SERI+LKL
Sbjct: 616 LEGRGTQSGAHVDIMGNFALIEDIMRVAAGGNGSDIGHDQVHSGIFEWSERIDLKL 671

The following BLAST results are available for this feature:

BLAST of Carg03188 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7010678.1	1.7e-139	100.00	putative phospholipid:diacylglycerol acyltransferase 2, partial [Cucurbita argyr...	[more]
KAG6570836.1	1.8e-138	99.59	putative phospholipid:diacylglycerol acyltransferase 2, partial [Cucurbita argyr...	[more]
XP_023512597.1	5.3e-138	99.19	putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita pepo subsp. pe...	[more]
XP_022986276.1	9.1e-138	98.78	putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita maxima]	[more]
XP_022944139.1	4.5e-137	98.37	putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita moschata]	[more]

BLAST of Carg03188 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FYC7	1.0e-99	71.54	Putative phospholipid:diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana O...	[more]
Q9FNA9	1.1e-80	61.02	Phospholipid:diacylglycerol acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN...	[more]
P40345	4.1e-24	31.51	Phospholipid:diacylglycerol acyltransferase OS=Saccharomyces cerevisiae (strain ...	[more]
O94680	7.0e-24	39.15	Phospholipid:diacylglycerol acyltransferase OS=Schizosaccharomyces pombe (strain...	[more]
Q08758	1.1e-05	29.79	Phosphatidylcholine-sterol acyltransferase OS=Papio anubis OX=9555 GN=LCAT PE=2 ...	[more]

BLAST of Carg03188 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JAN3	4.4e-138	98.78	putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucurbita maxima OX=36...	[more]
A0A6J1FW53	2.2e-137	98.37	putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucurbita moschata OX=...	[more]
A0A5A7SR19	7.1e-128	92.24	Putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucumis melo var. maku...	[more]
A0A1S3C9K8	7.1e-128	92.24	putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucumis melo OX=3656 G...	[more]
A0A0A0KGM0	9.2e-128	92.24	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G149960 PE=4 SV=1	[more]

BLAST of Carg03188 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G44830.1	7.3e-101	71.54	Lecithin:cholesterol acyltransferase family protein	[more]
AT5G13640.1	7.6e-82	61.02	phospholipid:diacylglycerol acyltransferase	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003386	Lecithin:cholesterol/phospholipid:diacylglycerol acyltransferase	PFAM	PF02450	LCAT	coord: 28..206 e-value: 2.2E-19 score: 69.9
None	No IPR available	PANTHER	PTHR11440	LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED	coord: 10..246
None	No IPR available	PANTHER	PTHR11440:SF87	PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE 2-RELATED	coord: 10..246

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg03188-RA	Carg03188-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008374	O-acyltransferase activity