Carg03169 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg03169
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	indole-3-acetic acid-amido synthetase GH3.10
Location	Carg_Chr20: 2755672 .. 2759142 (+)
RNA-Seq Expression	Carg03169
Synteny	Carg03169

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTGCTGCTGCTGCGGCTGCTGCGGCTGCGGGCGTGTTCAAGGCGGCGAGGTGCGCACCATGTTTGAAGAAGTAGAAGACATGGTTGATTGGTTCGAGCGAGTGTCGTCAGAAGCAGGCGTGGCTCAATTGCGCACACTCCGCGAGATTCTGCGCCAGAATTGTGGCGTGGAGTATCTAAACCAATGGCTTGGAGATGTCAATATCCATCAAATTCCTGATGACTTTGCATTGGAATCCCTCTTCACTTCCTTAATCCCCCTTTCTTCCCACGCTGATTTCGAGCCTTTTCTTCAACGAATCGCCGATGGTGACTCTTCCCCTTTGCTTACACAACAACCCATTCAAACTCTCTCTTTAAGGTATACCCTTCTTCAACATTTTCCCTTGCATTTTATGAATCCCTTCTTTATTACAAATGATATCATTACGCCGCTGCAGTTCTGGCACCACCGAAGGCCGACAAAAGTACGTGCCCTTCACTCCACATAGTGCGCAGACTACCCTTCTCATCTTTCGTATAGCTGCTGCTTATAGATCCAGGTACCAATGCAATATGGTCAGAATCTTCATTTTTTTTGAGTTACCCTTTTCTTGCAAGACATTAAAGAATGGCTTAATCACAACTAGGGTTTATCCCACAAGGGAAGGAGGCAGGATTTTGGAGTTCATTTACAGTAGTAAACAGACCAAAACCAAAGGAGGGATAACCACCGGCACAGCCACAACCCATTACTACGCCAGTGAGCAATTCAAAATCAAGCAGCTCAAAACCAAATCCTTCACTTGCAGCCCACAAGAAGTCATTTCCGGCGGTGATTACAAGCAATCCACTTACTGTCATCTTCTATTGGGGCTGTTCTTCTCCGACCAAGTCGAGTTTGTCACCTCAACTTTCGCTTACACCATAGTCCAAGCCTTCAACGAATTGGAAGATTGTTGGCCTCAAATCTGTCACGACATTACCCATGCCACACTCTGCTCTAGAATCACTCTTCCTGAAATCCGTAAGGCTGTTTTGAACATCATTAGTCCAAACCCAATTCTTGGGGCGAAAATTCAAGCTGTTTGTGAAGAGCTTGAGAGACAGGATTGGTTGGATTTGGTTCCGAAGCTATGGCCGAATTGTAAGTATGTTTACTCCATAATGACGGGGTCTATGCAGCCGTATTTGAAGAAATTGAGACGCTATGCGGGGAGGTTGCCGTTGGTGAGTGGGGATTATGGATCGACAGAGAGTTGGATTGGGGTTAATGTCGATCCTTGTCTGCCGCCTGAGAATGTTACATTTGCGGTCGTACCCACTTTCTCTTATTTTGAGTTCATCCCACTTTTCAGACAACATCAACAACATGATTGTGGCAGTTCCTCCGTTGCTGTCTTCGATGATTTCTTGGAAGGCCAACCCCTTCCCTTGTCTCAAGTCAAGATTGGACAGCAGTACGAACTTGTTCTCACCACTTTCACAGGTTAGTTCATTAATTATCAACACAAATCTAATCAAACGCCTTGCTTTTGCTCCTTCCTTATCATTATGTCATCCAGAGAAGATTAGTTTTTAAAACACGTTTGCTAGGGAGAGTTGCTTCGTTTGATCTCCCAATTCACTTCTCTTGGAGACCCACACATAGTTCAATGTCTAGTTTTGATACGAACTGTAATGACCCGACTAACTCTGACCGCTATGTGTAAGAAAAGTTGAGCAGTTACCATTAGTATCATGATTTGGTAATCATGAATCATTGATTTGATCAGTAATGATCAGTCCTATACGGGCAAGTTGAGAGAAATTTCCAACCTTCCTTATTTGGCTCCAAAATTTCCCAACTTTCCTTGTCAACATGATGCTTACATTGATTTCGCAAAGGTAAAATTTTGTACCAAGATTTGTTTTTTATTGAAATTTAGTGGGTGAATATCAATAAAGCCTAAAAAATGTGAAAACTTTTTTACTATAGTTTACCGTCACATCAAATATAGAGCATCTGATTGTAAATATGTAACCGCTCAAGCCCATTACTACTTGATATTATCCTCTTTAGACTTTCTCTCTCGAGCTTCCCCTAAAATGCATCTTTAGACTTTCACTTTCAAGCTTCCCCTCATTCTTTACTAAATTTTTCTGTCACATCAAATATAGAGCATATAGATCATATGATTTAATATATAATAGCTCGAGTATTATATTCTTTGTGTTTTTCTTTACGATCTTCTCTTCAAGGCTTTTAAGACATGTCTACTAGGAATAGGTTCTTACGTACTACTTATAAGGAGTGTTTCATTCTCATCTCTAAAAGAGGTGTTTAAACATAGTTCAGATGATTATGACATATATGTTTGATCTTCGACCCAAAGGGGTTAGTGGGTTTTGGTATTGTTTGCTCAACGGATGATTATGACATATATCTTCGATCGAAAGGGGTTAATGGGTTTTGGGTATTGGTTGTGATTGGCAGGACTATACAGATGCAGATTAGGAGACGTAGTAGAAGTAGCAGGGTTTCACAACAAGACACCTAAACTCAACTTTATATGCAGAAGAAAGCTTGTATTAACCGTAAACATTGACAAGAACACCGAGAAGGATCTCCAATTGGTAGTGGAAAGAGGGTCCCAGCTGCTAGCCGACCAAAGTGGAGCCGAATTAGTTGACTTCACCAGCCATGCCGACTTAACCAACCAACCAGGCCACTATGTCATCTTCTGGGAAGTCAAAGGGCACGTCGAGGACGCAGTTCTTGGACACTGCTGCTCCGAGATGGACGCTGCTTTCGTGGACTACGGCTACGTGGTGTCCAGGCGGTCCAACACCATCGGACCCCTCGAGCTAAGGATCGTGGAAAGAGGCAGCTTCAACAAGATCATGGAACATTACATTGGTAACGGAGCTGCACTTAGCCAATTCAAGACACCCAGATGCACTACCAATCAGGTTCTTCTTACCATTCTCAATCTCTCCACTGTCAAAGCCTTTCATAGCACTGCTTATGGCTAAGTTTGGTTATTAGTATTGTTAGTATTTCCCAATTTTGAATCAGTTTCAGATCATTTCATTCTAACCAAATTAGAGTGTCACTAAACACTTACTCATCACCGCATGTAACGAGCCAAAACGAATAATATTTGTTAGTGGTGGGTTTCGGTTGTTACAATTAGTATCAGAGATAGACACCGAGCTGTCGTATCAGTTGGAGAAGAGAACAAATCATTCCTTATAGATGCTATTATAGTACCTCAAGAGTGAATAAAGTTTCGGTCGTCGTCGTCCTCGTCGTCTTGTTGTTTTTGCTTTTATCATCATAAAGCTGCCTCAACTCTTCTTCCACTGCTAGCTCTTAATGGGTTTGCTTTTGATTTCTACTAAAATCCTCCATTTGTGCATTATTTACTGCTACACCCCTCCCCTTTTCCGTCTCCTATGATATAATTATAAGGTTAAAAATGTAAATTTGTTAACTAATATTACTTTTTTT

mRNA sequence

CTGCTGCTGCTGCGGCTGCTGCGGCTGCGGGCGTGTTCAAGGCGGCGAGGTGCGCACCATGTTTGAAGAAGTAGAAGACATGGTTGATTGGTTCGAGCGAGTGTCGTCAGAAGCAGGCGTGGCTCAATTGCGCACACTCCGCGAGATTCTGCGCCAGAATTGTGGCGTGGAGTATCTAAACCAATGGCTTGGAGATGTCAATATCCATCAAATTCCTGATGACTTTGCATTGGAATCCCTCTTCACTTCCTTAATCCCCCTTTCTTCCCACGCTGATTTCGAGCCTTTTCTTCAACGAATCGCCGATGGTGACTCTTCCCCTTTGCTTACACAACAACCCATTCAAACTCTCTCTTTAAGTTCTGGCACCACCGAAGGCCGACAAAAGTACGTGCCCTTCACTCCACATAGTGCGCAGACTACCCTTCTCATCTTTCGTATAGCTGCTGCTTATAGATCCAGGTACCAATGCAATATGACATTAAAGAATGGCTTAATCACAACTAGGGTTTATCCCACAAGGGAAGGAGGCAGGATTTTGGAGTTCATTTACAGTAGTAAACAGACCAAAACCAAAGGAGGGATAACCACCGGCACAGCCACAACCCATTACTACGCCAGTGAGCAATTCAAAATCAAGCAGCTCAAAACCAAATCCTTCACTTGCAGCCCACAAGAAGTCATTTCCGGCGGTGATTACAAGCAATCCACTTACTGTCATCTTCTATTGGGGCTGTTCTTCTCCGACCAAGTCGAGTTTGTCACCTCAACTTTCGCTTACACCATAGTCCAAGCCTTCAACGAATTGGAAGATTGTTGGCCTCAAATCTGTCACGACATTACCCATGCCACACTCTGCTCTAGAATCACTCTTCCTGAAATCCGTAAGGCTGTTTTGAACATCATTAGTCCAAACCCAATTCTTGGGGCGAAAATTCAAGCTGTTTGTGAAGAGCTTGAGAGACAGGATTGGTTGGATTTGGTTCCGAAGCTATGGCCGAATTGTAAGTATGTTTACTCCATAATGACGGGGTCTATGCAGCCGTATTTGAAGAAATTGAGACGCTATGCGGGGAGGTTGCCGTTGGTGAGTGGGGATTATGGATCGACAGAGAGTTGGATTGGGGTTAATGTCGATCCTTGTCTGCCGCCTGAGAATGTTACATTTGCGGTCGTACCCACTTTCTCTTATTTTGAGTTCATCCCACTTTTCAGACAACATCAACAACATGATTGTGGCAGTTCCTCCGTTGCTGTCTTCGATGATTTCTTGGAAGGCCAACCCCTTCCCTTGTCTCAAGTCAAGATTGGACAGCAGTACGAACTTGTTCTCACCACTTTCACAGGACTATACAGATGCAGATTAGGAGACGTAGTAGAAGTAGCAGGGTTTCACAACAAGACACCTAAACTCAACTTTATATGCAGAAGAAAGCTTGTATTAACCGTAAACATTGACAAGAACACCGAGAAGGATCTCCAATTGGTAGTGGAAAGAGGGTCCCAGCTGCTAGCCGACCAAAGTGGAGCCGAATTAGTTGACTTCACCAGCCATGCCGACTTAACCAACCAACCAGGCCACTATGTCATCTTCTGGGAAGTCAAAGGGCACGTCGAGGACGCAGTTCTTGGACACTGCTGCTCCGAGATGGACGCTGCTTTCGTGGACTACGGCTACGTGGTGTCCAGGCGGTCCAACACCATCGGACCCCTCGAGCTAAGGATCGTGGAAAGAGGCAGCTTCAACAAGATCATGGAACATTACATTGGTAACGGAGCTGCACTTAGCCAATTCAAGACACCCAGATGCACTACCAATCAGGTTCTTCTTACCATTCTCAATCTCTCCACTGTCAAAGCCTTTCATAGCACTGCTTATGGCTAAGTTTGGTTATTAGTATTGTTAGTATTTCCCAATTTTGAATCAGTTTCAGATCATTTCATTCTAACCAAATTAGAGTGTCACTAAACACTTACTCATCACCGCATGTAACGAGCCAAAACGAATAATATTTGTTAGTGGTGGGTTTCGGTTGTTACAATTAGTATCAGAGATAGACACCGAGCTGTCGTATCAGTTGGAGAAGAGAACAAATCATTCCTTATAGATGCTATTATAGTACCTCAAGAGTGAATAAAGTTTCGGTCGTCGTCGTCCTCGTCGTCTTGTTGTTTTTGCTTTTATCATCATAAAGCTGCCTCAACTCTTCTTCCACTGCTAGCTCTTAATGGGTTTGCTTTTGATTTCTACTAAAATCCTCCATTTGTGCATTATTTACTGCTACACCCCTCCCCTTTTCCGTCTCCTATGATATAATTATAAGGTTAAAAATGTAAATTTGTTAACTAATATTACTTTTTTT

Coding sequence (CDS)

ATGTTTGAAGAAGTAGAAGACATGGTTGATTGGTTCGAGCGAGTGTCGTCAGAAGCAGGCGTGGCTCAATTGCGCACACTCCGCGAGATTCTGCGCCAGAATTGTGGCGTGGAGTATCTAAACCAATGGCTTGGAGATGTCAATATCCATCAAATTCCTGATGACTTTGCATTGGAATCCCTCTTCACTTCCTTAATCCCCCTTTCTTCCCACGCTGATTTCGAGCCTTTTCTTCAACGAATCGCCGATGGTGACTCTTCCCCTTTGCTTACACAACAACCCATTCAAACTCTCTCTTTAAGTTCTGGCACCACCGAAGGCCGACAAAAGTACGTGCCCTTCACTCCACATAGTGCGCAGACTACCCTTCTCATCTTTCGTATAGCTGCTGCTTATAGATCCAGGTACCAATGCAATATGACATTAAAGAATGGCTTAATCACAACTAGGGTTTATCCCACAAGGGAAGGAGGCAGGATTTTGGAGTTCATTTACAGTAGTAAACAGACCAAAACCAAAGGAGGGATAACCACCGGCACAGCCACAACCCATTACTACGCCAGTGAGCAATTCAAAATCAAGCAGCTCAAAACCAAATCCTTCACTTGCAGCCCACAAGAAGTCATTTCCGGCGGTGATTACAAGCAATCCACTTACTGTCATCTTCTATTGGGGCTGTTCTTCTCCGACCAAGTCGAGTTTGTCACCTCAACTTTCGCTTACACCATAGTCCAAGCCTTCAACGAATTGGAAGATTGTTGGCCTCAAATCTGTCACGACATTACCCATGCCACACTCTGCTCTAGAATCACTCTTCCTGAAATCCGTAAGGCTGTTTTGAACATCATTAGTCCAAACCCAATTCTTGGGGCGAAAATTCAAGCTGTTTGTGAAGAGCTTGAGAGACAGGATTGGTTGGATTTGGTTCCGAAGCTATGGCCGAATTGTAAGTATGTTTACTCCATAATGACGGGGTCTATGCAGCCGTATTTGAAGAAATTGAGACGCTATGCGGGGAGGTTGCCGTTGGTGAGTGGGGATTATGGATCGACAGAGAGTTGGATTGGGGTTAATGTCGATCCTTGTCTGCCGCCTGAGAATGTTACATTTGCGGTCGTACCCACTTTCTCTTATTTTGAGTTCATCCCACTTTTCAGACAACATCAACAACATGATTGTGGCAGTTCCTCCGTTGCTGTCTTCGATGATTTCTTGGAAGGCCAACCCCTTCCCTTGTCTCAAGTCAAGATTGGACAGCAGTACGAACTTGTTCTCACCACTTTCACAGGACTATACAGATGCAGATTAGGAGACGTAGTAGAAGTAGCAGGGTTTCACAACAAGACACCTAAACTCAACTTTATATGCAGAAGAAAGCTTGTATTAACCGTAAACATTGACAAGAACACCGAGAAGGATCTCCAATTGGTAGTGGAAAGAGGGTCCCAGCTGCTAGCCGACCAAAGTGGAGCCGAATTAGTTGACTTCACCAGCCATGCCGACTTAACCAACCAACCAGGCCACTATGTCATCTTCTGGGAAGTCAAAGGGCACGTCGAGGACGCAGTTCTTGGACACTGCTGCTCCGAGATGGACGCTGCTTTCGTGGACTACGGCTACGTGGTGTCCAGGCGGTCCAACACCATCGGACCCCTCGAGCTAAGGATCGTGGAAAGAGGCAGCTTCAACAAGATCATGGAACATTACATTGGTAACGGAGCTGCACTTAGCCAATTCAAGACACCCAGATGCACTACCAATCAGGTTCTTCTTACCATTCTCAATCTCTCCACTGTCAAAGCCTTTCATAGCACTGCTTATGGCTAA

Protein sequence

MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG

Homology

[Close]

BLAST of Carg03169 vs. NCBI nr
Match: KAG7010696.1 (Indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1248.0 bits (3228), Expect = 0.0e+00
Identity = 608/608 (100.00%), Postives = 608/608 (100.00%), Query Frame = 0


Query: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGT 180
           TTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGT
Sbjct: 121 TTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGT 180

Query: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240
           ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA
Sbjct: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240

Query: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL 300
           YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL
Sbjct: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL 300

Query: 301 ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD 360
           ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD
Sbjct: 301 ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD 360

Query: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQ 420
           PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQ
Sbjct: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQ 420

Query: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480
           YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER
Sbjct: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480

Query: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY 540
           GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY
Sbjct: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY 540

Query: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 600
           VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK
Sbjct: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 600

Query: 601 AFHSTAYG 609
           AFHSTAYG
Sbjct: 601 AFHSTAYG 608

[Close]

BLAST of Carg03169 vs. NCBI nr
Match: KAG6570848.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1202.6 bits (3110), Expect = 0.0e+00
Identity = 591/608 (97.20%), Postives = 591/608 (97.20%), Query Frame = 0


Query: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGT 180
           TTLLIFRIAAAYRS               RVYPTREGGRILEFIYSSKQTKTKGGITTGT
Sbjct: 121 TTLLIFRIAAAYRS---------------RVYPTREGGRILEFIYSSKQTKTKGGITTGT 180

Query: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240
           ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA
Sbjct: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240

Query: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL 300
           YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKI AVCEEL
Sbjct: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEEL 300

Query: 301 ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD 360
           ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD
Sbjct: 301 ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD 360

Query: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQ 420
           PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVA FDDFLEGQPLPLSQVKIGQQ
Sbjct: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQ 420

Query: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480
           YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER
Sbjct: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480

Query: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY 540
           GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY
Sbjct: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY 540

Query: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 600
           VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK
Sbjct: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 593

Query: 601 AFHSTAYG 609
           AFHSTAYG
Sbjct: 601 AFHSTAYG 593

[Close]

BLAST of Carg03169 vs. NCBI nr
Match: XP_023512179.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1200.7 bits (3105), Expect = 0.0e+00
Identity = 589/608 (96.88%), Postives = 591/608 (97.20%), Query Frame = 0


Query: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSL+PLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGT 180
           TTLLIFRIAAAYRS               RVYPTREGGRILEFIYSSKQTKTKGGITTGT
Sbjct: 121 TTLLIFRIAAAYRS---------------RVYPTREGGRILEFIYSSKQTKTKGGITTGT 180

Query: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240
           ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA
Sbjct: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240

Query: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL 300
           YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKI AVCEEL
Sbjct: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEEL 300

Query: 301 ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD 360
           ERQDWLDLVPKLWPNCKYVYSIMTGS+QPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD
Sbjct: 301 ERQDWLDLVPKLWPNCKYVYSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD 360

Query: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQ 420
           PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVA FDDFLEGQPLPLSQVKIGQQ
Sbjct: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQ 420

Query: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480
           YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER
Sbjct: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480

Query: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY 540
           GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY
Sbjct: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY 540

Query: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 600
           VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK
Sbjct: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 593

Query: 601 AFHSTAYG 609
           AFHSTAYG
Sbjct: 601 AFHSTAYG 593

[Close]

BLAST of Carg03169 vs. NCBI nr
Match: XP_022943441.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita moschata])

HSP 1 Score: 1199.9 bits (3103), Expect = 0.0e+00
Identity = 589/608 (96.88%), Postives = 591/608 (97.20%), Query Frame = 0


Query: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFE+VEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPI+TLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGT 180
           TTLLIFRIAAAYRS               RVYPTREGGRILEFIYSSKQTKTKGGITTGT
Sbjct: 121 TTLLIFRIAAAYRS---------------RVYPTREGGRILEFIYSSKQTKTKGGITTGT 180

Query: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240
           ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA
Sbjct: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240

Query: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL 300
           YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKI AVCEEL
Sbjct: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEEL 300

Query: 301 ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD 360
           ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD
Sbjct: 301 ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD 360

Query: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQ 420
           PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVA FDDFLEGQPLPLSQVKIGQQ
Sbjct: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQ 420

Query: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480
           YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER
Sbjct: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480

Query: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY 540
           GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY
Sbjct: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY 540

Query: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 600
           VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK
Sbjct: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 593

Query: 601 AFHSTAYG 609
           AFHSTAYG
Sbjct: 601 AFHSTAYG 593

[Close]

BLAST of Carg03169 vs. NCBI nr
Match: XP_022986272.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita maxima])

HSP 1 Score: 1189.5 bits (3076), Expect = 0.0e+00
Identity = 584/608 (96.05%), Postives = 588/608 (96.71%), Query Frame = 0


Query: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFEEVEDMVDWFERVSSEAGVAQ RTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSL+PLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGT 180
           TTLLIFRIAAAYRS               RVYPTREGGRILEFIYSSKQTKTKGGITTGT
Sbjct: 121 TTLLIFRIAAAYRS---------------RVYPTREGGRILEFIYSSKQTKTKGGITTGT 180

Query: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240
           ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA
Sbjct: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240

Query: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL 300
           YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL
Sbjct: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL 300

Query: 301 ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD 360
           ERQDWLDLVPKLW NCKYVYSIMTGSMQPYLKKLRRYAG LPLVSGDYGSTESWIG+NVD
Sbjct: 301 ERQDWLDLVPKLWSNCKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVD 360

Query: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQ 420
           PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVA FDDFLEGQPLPLSQVKIGQQ
Sbjct: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQ 420

Query: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480
           YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER
Sbjct: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480

Query: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY 540
           GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKG+V+DAVLG CCSEMDAAFVDYGY
Sbjct: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGY 540

Query: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 600
           VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK
Sbjct: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 593

Query: 601 AFHSTAYG 609
           AFHSTAYG
Sbjct: 601 AFHSTAYG 593

[Close]

BLAST of Carg03169 vs. ExPASy Swiss-Prot
Match: Q9ZNS2 (Indole-3-acetic acid-amido synthetase GH3.10 OS=Arabidopsis thaliana OX=3702 GN=GH3.10 PE=2 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 7.8e-234
Identity = 396/603 (65.67%), Postives = 471/603 (78.11%), Query Frame = 0


Query: 6   EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 65
           +D++ WFE VS  A   Q  TLR IL  N GVEYL +WLG V++ ++ DD+ LE+LFTSL
Sbjct: 9   DDVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKM-DDYTLETLFTSL 68

Query: 66  IPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 125
           +P+ SHAD +P++QRIADG++SPLLTQ+PI  LSLSSGTTEGRQKYVPFT HSAQTTL I
Sbjct: 69  VPIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQTTLQI 128

Query: 126 FRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHY 185
           FR++AAYRSR+               YP REGGRILEFIY+ K+ KT GG+T GTATTHY
Sbjct: 129 FRLSAAYRSRF---------------YPIREGGRILEFIYAGKEFKTLGGLTVGTATTHY 188

Query: 186 YASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQ 245
           YASE+FK KQ  TKSFTCSPQEVISGGD+ Q TYCHLLLGL +S QVEFV S F+YTIVQ
Sbjct: 189 YASEEFKTKQETTKSFTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQ 248

Query: 246 AFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQ-D 305
           AF+  E+ W +IC DI    L SRITLP++RKAVL +I PNP L + I+ +C ELE    
Sbjct: 249 AFSFFEEIWREICADIKEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLG 308

Query: 306 WLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLP 365
           W  L+ KLWPN K++ SIMTGSM PYL KLR YAG LPLVS DYGSTESWIGVNVDP LP
Sbjct: 309 WFGLISKLWPNAKFISSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLP 368

Query: 366 PENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQYELV 425
           PE+V+FAV+PTFSYFEFIPL+R+  Q D     + +  DF+E +P+PLSQVK+GQ+YELV
Sbjct: 369 PEDVSFAVIPTFSYFEFIPLYRRQNQSD-----ICIDGDFVEDKPVPLSQVKLGQEYELV 428

Query: 426 LTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQL 485
           LTTFTGLYR RLGDVVEV  FH  TPKL+FI RRKL+LT+NIDKNTEKDLQ VV++ SQL
Sbjct: 429 LTTFTGLYRYRLGDVVEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQL 488

Query: 486 LADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSR 545
           L+  + AE+VDFTSHAD+  +PGHYVI+WE++G  +D  L  CC EMD AFVDYGYVVSR
Sbjct: 489 LSRSTRAEVVDFTSHADVIARPGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSR 548

Query: 546 RSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHS 605
           R N+IGPLELR+VERG+F K+ E  +G    L+QFKTPRCTTN V+L ILN ST+K F S
Sbjct: 549 RMNSIGPLELRVVERGTFGKVAERCVGKCGGLNQFKTPRCTTNSVMLDILNDSTIKRFRS 590

Query: 606 TAY 608
           +AY
Sbjct: 609 SAY 590

[Close]

BLAST of Carg03169 vs. ExPASy Swiss-Prot
Match: A0A1J6KGJ9 (Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 PE=1 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 3.1e-166
Identity = 294/604 (48.68%), Postives = 408/604 (67.55%), Query Frame = 0


Query: 6   EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 65
           E++++ FE ++ +AG  Q  TL++IL +N G EYL QW     ++   D  +    F + 
Sbjct: 14  EEVIEEFEVLTKDAGKIQEETLQKILEENGGTEYLQQW----GLNGKTDSLS----FKNC 73

Query: 66  IPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 125
           IP+ +H D EP++ RIADGD SP+LT +PI T+SLSSGTT+G+ K+VPF     ++T+ I
Sbjct: 74  IPIVTHKDLEPYIHRIADGDLSPILTGKPITTISLSSGTTQGKPKFVPFNEELMESTMQI 133

Query: 126 FRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHY 185
           F+ +  +R+               R +P    G+ L+FIY SKQ KTKGG+  GTATT+ 
Sbjct: 134 FKTSFVFRN---------------REFPV-VNGKALQFIYGSKQFKTKGGLAAGTATTNV 193

Query: 186 YASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQ 245
           Y + QFK      ++  CSP EVI G D++QS YCHLL GL F D+V+ V+STFA++IV 
Sbjct: 194 YRNAQFKKTMKAMQTPCCSPDEVIFGPDFQQSLYCHLLCGLIFRDEVQVVSSTFAHSIVH 253

Query: 246 AFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDW 305
           AF   E  W ++  +I    L SR+ +P +R A+  ++ P+P L   I   C  L   +W
Sbjct: 254 AFRNFEQIWQELVTNIREGVLSSRVIVPSMRAAMSKLLKPDPELADTIFNKCSRL--SNW 313

Query: 306 LDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPP 365
             L+P+L+PN +Y+Y IMTGSM+PYLKKLR YAG LPL+S DYGS+E WIG NV+P LPP
Sbjct: 314 YGLIPELFPNTRYIYGIMTGSMEPYLKKLRHYAGDLPLLSADYGSSEGWIGANVNPELPP 373

Query: 366 ENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQYELVL 425
           E VT+AV+P   YFEFIPL                  D LE  P+ L++VK+G++YE+V+
Sbjct: 374 ELVTYAVLPNIGYFEFIPLMENL--------------DGLEPMPVGLTEVKLGEEYEIVV 433

Query: 426 TTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLL 485
           T F GLYR RLGDVV++ GFHN TP+L FICRR L+L++NIDKNTEKDLQL VE  +++L
Sbjct: 434 TNFAGLYRYRLGDVVKIKGFHNGTPELQFICRRNLLLSINIDKNTEKDLQLAVEAAAKIL 493

Query: 486 ADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRR 545
           +D+   E+VDFTSH +++  PGHYVIFWE+ G   + +L  CC+ +D +FVD GYV SR+
Sbjct: 494 SDEK-LEVVDFTSHVNVSADPGHYVIFWELNGEASEEILKECCNCLDKSFVDAGYVGSRK 553

Query: 546 SNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFH 605
            + IG LELRIV+RG+F+KI++H++G GAA+SQFKTPRC   TN  +L IL+ + V+++ 
Sbjct: 554 VHAIGALELRIVKRGTFHKILDHFVGLGAAVSQFKTPRCVGPTNLSVLQILSSNVVESYF 576

Query: 606 STAY 608
           STA+
Sbjct: 614 STAF 576

[Close]

BLAST of Carg03169 vs. ExPASy Swiss-Prot
Match: A0A314KSQ4 (Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 PE=1 SV=2)

HSP 1 Score: 582.0 bits (1499), Expect = 7.7e-165
Identity = 297/604 (49.17%), Postives = 400/604 (66.23%), Query Frame = 0


Query: 6   EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 65
           E +++ FE ++ +AG  Q  TL++IL QN G EYL  W   +N    P        F + 
Sbjct: 15  EKVIEEFEDLTKDAGKIQEETLKKILEQNGGTEYLQLW--GLNGRTDP------QTFKNC 74

Query: 66  IPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 125
           +P+ +H D EP++QRIADGD SP+LT +PI+T+SLSSGTT+G+ K+VPF     ++T+ I
Sbjct: 75  VPIVTHNDLEPYIQRIADGDLSPILTGKPIETISLSSGTTQGKPKFVPFNDELMESTMQI 134

Query: 126 FRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHY 185
           F+ + A+R+               R +P    G+ L+FIYSSKQ KTKGG+  GTATT+ 
Sbjct: 135 FKTSFAFRN---------------REFPI-GNGKALQFIYSSKQFKTKGGLAAGTATTNV 194

Query: 186 YASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQ 245
           Y + QFK       +  CSP EVI G D+ QS YCHLL GL F D+V+ V+STFA++IV 
Sbjct: 195 YRNAQFKKTMKAMCTPCCSPDEVIFGPDFHQSLYCHLLCGLIFHDEVQVVSSTFAHSIVH 254

Query: 246 AFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDW 305
           AF   E  W  +  DI    L SR+T+P IR A+  ++ P+P L   I   C  L   +W
Sbjct: 255 AFRTFEQVWEALVVDIREGVLSSRVTVPSIRLAMSKLLKPDPELADTIYNKCSRL--SNW 314

Query: 306 LDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPP 365
             L+P L+PN +Y+Y IMTGSM+PYLKKLR YAG LPL+S DYGS+E W+GVNV+P LPP
Sbjct: 315 YGLIPDLFPNTRYIYGIMTGSMEPYLKKLRHYAGELPLLSADYGSSEGWVGVNVNPKLPP 374

Query: 366 ENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQYELVL 425
           E VT+AV+P   YFEFIPL       +  +S            P+ L++VK+G++YE+V 
Sbjct: 375 ELVTYAVLPNIGYFEFIPLGGNLNGIEQANS------------PVGLTEVKLGEEYEVVF 434

Query: 426 TTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLL 485
           T F GLYR RLGDVV+V GFHN TP+L F+CR  L+L++NIDKNTEKDLQL VE  ++ L
Sbjct: 435 TNFAGLYRYRLGDVVKVKGFHNGTPELQFVCRSNLLLSINIDKNTEKDLQLAVEAAAKRL 494

Query: 486 ADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRR 545
            D+   E+VDFTSH +++  PGHYVIFWE+ G   D +L  CC+ +D +F+D GYV SR+
Sbjct: 495 VDEK-LEVVDFTSHVNVSADPGHYVIFWELSGEATDEMLQDCCNCLDRSFIDAGYVSSRK 554

Query: 546 SNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFH 605
            N IG LELRIV+RG+F+KI++H++G G A+SQFKTPRC    N  LL IL+ + V+ + 
Sbjct: 555 VNAIGALELRIVKRGTFHKILDHFVGLGGAVSQFKTPRCVGPKNSSLLQILSSNVVETYV 579

Query: 606 STAY 608
           STA+
Sbjct: 615 STAF 579

[Close]

BLAST of Carg03169 vs. ExPASy Swiss-Prot
Match: Q6I581 (Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 1.3e-159
Identity = 287/606 (47.36%), Postives = 392/606 (64.69%), Query Frame = 0


Query: 6   EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYL-NQWLGDVNIHQIPDDFALESLFTS 65
           E+ ++ FE ++ +A   Q  TL++IL  N   EYL N  LG     +          + S
Sbjct: 7   EETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNFGLGGRTDAE---------SYKS 66

Query: 66  LIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLL 125
            IPL  H D EP++QRI DGD+SP++T +PI  LSLSSGTT G+ K++PF     +TTL 
Sbjct: 67  CIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKPKFIPFNDELLETTLQ 126

Query: 126 IFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTH 185
           I+R + A+R+               R YP  + G+ L+F+Y SKQ  TKGGI   TATT+
Sbjct: 127 IYRTSYAFRN---------------REYPIGQ-GKALQFVYGSKQVITKGGILATTATTN 186

Query: 186 YYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIV 245
            Y  +++K      +S  CSP EVI G D+ QS YCHLL GL +S++V  V STFA+++V
Sbjct: 187 LYRRQRYKEGMKDIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAHSLV 246

Query: 246 QAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQD 305
            AF   E+ W  +C DI    L  ++T P IR+AV  I+ PNP L   I   C  L   +
Sbjct: 247 HAFQTFEEVWEDLCTDIRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIGL--SN 306

Query: 306 WLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLP 365
           W  ++P LWPN KYVY IMTGSM+PYLKKLR YAG LPL+S DYG++E W+G N+DP +P
Sbjct: 307 WYGVIPALWPNAKYVYGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNIDPTVP 366

Query: 366 PENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQYELV 425
           PE VT+AV+P   YFEFIPL +   +    S+S+     ++E  P+ L++V++G+ YE+V
Sbjct: 367 PEQVTYAVLPQVGYFEFIPLEKPIGEETENSASI----HYIESDPVGLTEVEVGKIYEVV 426

Query: 426 LTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQL 485
           +T F GLYR RLGDVV++A FHN TP+L FICRR LVL++NIDKNTEKDLQL VE  S+ 
Sbjct: 427 ITNFAGLYRYRLGDVVKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVEEASKF 486

Query: 486 LADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSR 545
           L  +   E++DFTS  + ++ PG YVIFWE+ G   D VL  C + +D AF+D GY  SR
Sbjct: 487 LEGEK-LEVMDFTSFVERSSDPGRYVIFWELSGDASDEVLSSCANALDLAFIDAGYTGSR 546

Query: 546 RSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAF 605
           +  TIGPLELRI+ +G+F +I++H++  G A+SQFKTPR    +N  +L IL+ +  +++
Sbjct: 547 KIKTIGPLELRILRKGTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQILSRNVTQSY 580

Query: 606 HSTAYG 609
            STAYG
Sbjct: 607 FSTAYG 580

[Close]

BLAST of Carg03169 vs. ExPASy Swiss-Prot
Match: Q9SKE2 (Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 PE=1 SV=2)

HSP 1 Score: 538.1 bits (1385), Expect = 1.3e-151
Identity = 276/614 (44.95%), Postives = 387/614 (63.03%), Query Frame = 0


Query: 1   MFEEVED-----MVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDD 60
           M E+VE      ++D F+ ++  A   Q +TL+EIL +N    YL       N     + 
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQ------NCGLNGNA 60

Query: 61  FALESLFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFT 120
              E  F S++PL +  + EP+++R+ DGD+SP+LT  P+  +SLSSGT++GR K++PFT
Sbjct: 61  TDPEEAFKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFT 120

Query: 121 PHSAQTTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGG 180
               + TL +FR A A+R+               R +P  + G+ L+FI+SSKQ  + GG
Sbjct: 121 DELMENTLQLFRTAFAFRN---------------RDFPIDDNGKALQFIFSSKQYISTGG 180

Query: 181 ITTGTATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFV 240
           +  GTATT+ Y +  FK       S +CSP EVI   D  Q+ YCHLL G+ F DQV++V
Sbjct: 181 VPVGTATTNVYRNPNFKAGMKSITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYV 240

Query: 241 TSTFAYTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQA 300
            + FA+ +V AF   E  W +I  DI    L +RIT+P +R A+  +++PNP L   I+ 
Sbjct: 241 FAVFAHGLVHAFRTFEQVWEEIVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRT 300

Query: 301 VCEELERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWI 360
            C  L   +W  L+P L+PN KYVY IMTGSM+PY+ KLR YAG LPLVS DYGS+E WI
Sbjct: 301 KCMSL--SNWYGLIPALFPNAKYVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWI 360

Query: 361 GVNVDPCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQV 420
             NV P L PE  TFAV+P   YFEF+P+       + G           E +P+ L+QV
Sbjct: 361 AANVTPRLSPEEATFAVIPNLGYFEFLPV------SETGEG---------EEKPVGLTQV 420

Query: 421 KIGQQYELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQ 480
           KIG++YE+V+T + GLYR RLGDVV+V GF+N TP+L FICRR L+L++NIDKNTE+DLQ
Sbjct: 421 KIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQ 480

Query: 481 LVVERGSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAF 540
           L VE  ++ L+++   E++DF+S+ D++  PGHY IFWE+ G   + VL  CC+ +D AF
Sbjct: 481 LSVESAAKRLSEEK-IEVIDFSSYIDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAF 540

Query: 541 VDYGYVVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTI 600
           +D GYV SR+  TIG LELR+V +G+F KI EH++G G++  QFK PRC   +N  +L I
Sbjct: 541 IDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQI 575

Query: 601 LNLSTVKAFHSTAY 608
           L  + V ++ STA+
Sbjct: 601 LCENVVSSYFSTAF 575

[Close]

BLAST of Carg03169 vs. ExPASy TrEMBL
Match: A0A6J1FRQ1 (indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita moschata OX=3662 GN=LOC111448207 PE=3 SV=1)

HSP 1 Score: 1199.9 bits (3103), Expect = 0.0e+00
Identity = 589/608 (96.88%), Postives = 591/608 (97.20%), Query Frame = 0


Query: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFE+VEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPI+TLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGT 180
           TTLLIFRIAAAYRS               RVYPTREGGRILEFIYSSKQTKTKGGITTGT
Sbjct: 121 TTLLIFRIAAAYRS---------------RVYPTREGGRILEFIYSSKQTKTKGGITTGT 180

Query: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240
           ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA
Sbjct: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240

Query: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL 300
           YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKI AVCEEL
Sbjct: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEEL 300

Query: 301 ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD 360
           ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD
Sbjct: 301 ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD 360

Query: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQ 420
           PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVA FDDFLEGQPLPLSQVKIGQQ
Sbjct: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQ 420

Query: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480
           YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER
Sbjct: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480

Query: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY 540
           GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY
Sbjct: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY 540

Query: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 600
           VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK
Sbjct: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 593

Query: 601 AFHSTAYG 609
           AFHSTAYG
Sbjct: 601 AFHSTAYG 593

[Close]

BLAST of Carg03169 vs. ExPASy TrEMBL
Match: A0A6J1J741 (indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita maxima OX=3661 GN=LOC111484069 PE=3 SV=1)

HSP 1 Score: 1189.5 bits (3076), Expect = 0.0e+00
Identity = 584/608 (96.05%), Postives = 588/608 (96.71%), Query Frame = 0


Query: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFEEVEDMVDWFERVSSEAGVAQ RTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSL+PLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGT 180
           TTLLIFRIAAAYRS               RVYPTREGGRILEFIYSSKQTKTKGGITTGT
Sbjct: 121 TTLLIFRIAAAYRS---------------RVYPTREGGRILEFIYSSKQTKTKGGITTGT 180

Query: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240
           ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA
Sbjct: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240

Query: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL 300
           YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL
Sbjct: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL 300

Query: 301 ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD 360
           ERQDWLDLVPKLW NCKYVYSIMTGSMQPYLKKLRRYAG LPLVSGDYGSTESWIG+NVD
Sbjct: 301 ERQDWLDLVPKLWSNCKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVD 360

Query: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQ 420
           PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVA FDDFLEGQPLPLSQVKIGQQ
Sbjct: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQ 420

Query: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480
           YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER
Sbjct: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480

Query: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY 540
           GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKG+V+DAVLG CCSEMDAAFVDYGY
Sbjct: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGY 540

Query: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 600
           VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK
Sbjct: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 593

Query: 601 AFHSTAYG 609
           AFHSTAYG
Sbjct: 601 AFHSTAYG 593

[Close]

BLAST of Carg03169 vs. ExPASy TrEMBL
Match: A0A5D3B9T4 (Jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold464G00300 PE=3 SV=1)

HSP 1 Score: 1008.1 bits (2605), Expect = 1.6e-290
Identity = 493/605 (81.49%), Postives = 540/605 (89.26%), Query Frame = 0


Query: 3   EEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLF 62
           EE ED++DWFE+VSSEA VAQ RTLR+IL+QN GVEYL +W+GDVNIH+IPDDF L+S+F
Sbjct: 8   EEEEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDDFTLQSIF 67

Query: 63  TSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTT 122
           TSLIPLSSHA FEPFLQRIADGDSSPLLTQQPI TLSLSSGTTEGRQKYVPFTPHSA TT
Sbjct: 68  TSLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTPHSAHTT 127

Query: 123 LLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTAT 182
           LLIFR+AAAYRS               RVYPTREGG+ILEFIYSSKQTKTKGGITTGTAT
Sbjct: 128 LLIFRLAAAYRS---------------RVYPTREGGKILEFIYSSKQTKTKGGITTGTAT 187

Query: 183 THYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYT 242
           THYYASE+FKIKQLKTKSFTCSPQEVISG DYKQSTYCHLLLGL +S++VEFVTSTFAYT
Sbjct: 188 THYYASEEFKIKQLKTKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYT 247

Query: 243 IVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELER 302
           IVQAFN+LE+ W +I HDI+HATL SRI +PEIRKAVL ++SP P LG KI+ VCEEL++
Sbjct: 248 IVQAFNQLEESWEEIIHDISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKK 307

Query: 303 QDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPC 362
           + WL L+PKLWPNCKYVYSIMTGSMQPYLKKLR Y G LPLVSGDYGSTESWIGVNVDP 
Sbjct: 308 EGWLGLIPKLWPNCKYVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPY 367

Query: 363 LPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQYE 422
           LPPENVTFAV+PTFSYFEFIPLF+QH      SS+     DFLEGQP+ LS+VKIGQQYE
Sbjct: 368 LPPENVTFAVIPTFSYFEFIPLFKQHA---TTSSTDTAIHDFLEGQPIALSEVKIGQQYE 427

Query: 423 LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGS 482
           LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKD+QL VERGS
Sbjct: 428 LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGS 487

Query: 483 QLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVV 542
           QL++  SGAELVDFTS+A+L+NQPGHYVIFWEVKG V D VLG CCSEMDAAFVDYGYVV
Sbjct: 488 QLMSQLSGAELVDFTSYAELSNQPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVV 547

Query: 543 SRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAF 602
           SR++N+IGPLELRIVERGSFNKI+E+YIGNGAALSQFKTPRCTTN  LL ILNLST+++F
Sbjct: 548 SRKANSIGPLELRIVERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSF 594

Query: 603 HSTAY 608
            STAY
Sbjct: 608 FSTAY 594

[Close]

BLAST of Carg03169 vs. ExPASy TrEMBL
Match: A0A1S3BIL6 (jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490483 PE=3 SV=1)

HSP 1 Score: 1008.1 bits (2605), Expect = 1.6e-290
Identity = 493/605 (81.49%), Postives = 540/605 (89.26%), Query Frame = 0


Query: 3   EEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLF 62
           EE ED++DWFE+VSSEA VAQ RTLR+IL+QN GVEYL +W+GDVNIH+IPDDF L+S+F
Sbjct: 8   EEEEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDDFTLQSIF 67

Query: 63  TSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTT 122
           TSLIPLSSHA FEPFLQRIADGDSSPLLTQQPI TLSLSSGTTEGRQKYVPFTPHSA TT
Sbjct: 68  TSLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTPHSAHTT 127

Query: 123 LLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTAT 182
           LLIFR+AAAYRS               RVYPTREGG+ILEFIYSSKQTKTKGGITTGTAT
Sbjct: 128 LLIFRLAAAYRS---------------RVYPTREGGKILEFIYSSKQTKTKGGITTGTAT 187

Query: 183 THYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYT 242
           THYYASE+FKIKQLKTKSFTCSPQEVISG DYKQSTYCHLLLGL +S++VEFVTSTFAYT
Sbjct: 188 THYYASEEFKIKQLKTKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYT 247

Query: 243 IVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELER 302
           IVQAFN+LE+ W +I HDI+HATL SRI +PEIRKAVL ++SP P LG KI+ VCEEL++
Sbjct: 248 IVQAFNQLEESWEEIIHDISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKK 307

Query: 303 QDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPC 362
           + WL L+PKLWPNCKYVYSIMTGSMQPYLKKLR Y G LPLVSGDYGSTESWIGVNVDP 
Sbjct: 308 EGWLGLIPKLWPNCKYVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPY 367

Query: 363 LPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQYE 422
           LPPENVTFAV+PTFSYFEFIPLF+QH      SS+     DFLEGQP+ LS+VKIGQQYE
Sbjct: 368 LPPENVTFAVIPTFSYFEFIPLFKQHA---TTSSTDTAIHDFLEGQPIALSEVKIGQQYE 427

Query: 423 LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGS 482
           LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKD+QL VERGS
Sbjct: 428 LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGS 487

Query: 483 QLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVV 542
           QL++  SGAELVDFTS+A+L+NQPGHYVIFWEVKG V D VLG CCSEMDAAFVDYGYVV
Sbjct: 488 QLMSQLSGAELVDFTSYAELSNQPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVV 547

Query: 543 SRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAF 602
           SR++N+IGPLELRIVERGSFNKI+E+YIGNGAALSQFKTPRCTTN  LL ILNLST+++F
Sbjct: 548 SRKANSIGPLELRIVERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSF 594

Query: 603 HSTAY 608
            STAY
Sbjct: 608 FSTAY 594

[Close]

BLAST of Carg03169 vs. ExPASy TrEMBL
Match: A0A5A7VB01 (Jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold301G00260 PE=3 SV=1)

HSP 1 Score: 995.7 bits (2573), Expect = 8.3e-287
Identity = 490/605 (80.99%), Postives = 539/605 (89.09%), Query Frame = 0


Query: 3   EEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLF 62
           EE ED++DWFE+VSSEA VAQ RTLR+IL+QN GVEYL +W+GDVNIH+IPDDF L+S+F
Sbjct: 8   EEEEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDDFTLQSIF 67

Query: 63  TSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTT 122
           TSLIPLSSHA FEPFLQRIADGDSSPLLTQQPI TLSLSSGTTEGRQKYVPFTPHSAQTT
Sbjct: 68  TSLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTPHSAQTT 127

Query: 123 LLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTAT 182
           LLIFR+AAAYRS    N+T K  L+T  +        ILEFIYSSKQTKTKGGITTGTAT
Sbjct: 128 LLIFRLAAAYRS---TNITQKQHLLTIYLL-------ILEFIYSSKQTKTKGGITTGTAT 187

Query: 183 THYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYT 242
           THYYASE+FKIKQLKTKSFTCSPQEVISG DYKQSTYCHLLLGL +S++VEFVTSTFAYT
Sbjct: 188 THYYASEEFKIKQLKTKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYT 247

Query: 243 IVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELER 302
           IVQAFN+LE+ W +I HDI+HATL SRI +PEIRKAVL ++SP P LG KI+ VCEEL++
Sbjct: 248 IVQAFNQLEESWEEIIHDISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKK 307

Query: 303 QDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPC 362
           + WL L+PKLWPNCKYVYSIMTGSMQPYLKKLR Y G LPLVSGDYGSTESWIGVNVDP 
Sbjct: 308 EGWLGLIPKLWPNCKYVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPY 367

Query: 363 LPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQYE 422
           LPPENVTFAV+PTFSYFEFIPLF+QH      SS+     DFLEGQP+ LS+VKIGQQYE
Sbjct: 368 LPPENVTFAVIPTFSYFEFIPLFKQHA---TTSSTDTAIHDFLEGQPIALSEVKIGQQYE 427

Query: 423 LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGS 482
           LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKD+QL VERGS
Sbjct: 428 LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGS 487

Query: 483 QLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVV 542
           QL++  SGAELVDFTS+A+L+NQPGHYVIFWEVKG V D VLG CCSEMDAAFVDYGYVV
Sbjct: 488 QLMSQLSGAELVDFTSYAELSNQPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVV 547

Query: 543 SRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAF 602
           SR++N+IGPLELRIVERGSFNKI+E+YIGNGAALSQFKTPRCTTN  LL ILNLST+++F
Sbjct: 548 SRKANSIGPLELRIVERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSF 599

Query: 603 HSTAY 608
            STAY
Sbjct: 608 FSTAY 599

[Close]

BLAST of Carg03169 vs. TAIR 10
Match: AT4G03400.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 811.2 bits (2094), Expect = 5.6e-235
Identity = 396/603 (65.67%), Postives = 471/603 (78.11%), Query Frame = 0


Query: 6   EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 65
           +D++ WFE VS  A   Q  TLR IL  N GVEYL +WLG V++ ++ DD+ LE+LFTSL
Sbjct: 9   DDVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKM-DDYTLETLFTSL 68

Query: 66  IPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 125
           +P+ SHAD +P++QRIADG++SPLLTQ+PI  LSLSSGTTEGRQKYVPFT HSAQTTL I
Sbjct: 69  VPIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQTTLQI 128

Query: 126 FRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHY 185
           FR++AAYRSR+               YP REGGRILEFIY+ K+ KT GG+T GTATTHY
Sbjct: 129 FRLSAAYRSRF---------------YPIREGGRILEFIYAGKEFKTLGGLTVGTATTHY 188

Query: 186 YASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQ 245
           YASE+FK KQ  TKSFTCSPQEVISGGD+ Q TYCHLLLGL +S QVEFV S F+YTIVQ
Sbjct: 189 YASEEFKTKQETTKSFTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQ 248

Query: 246 AFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQ-D 305
           AF+  E+ W +IC DI    L SRITLP++RKAVL +I PNP L + I+ +C ELE    
Sbjct: 249 AFSFFEEIWREICADIKEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLG 308

Query: 306 WLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLP 365
           W  L+ KLWPN K++ SIMTGSM PYL KLR YAG LPLVS DYGSTESWIGVNVDP LP
Sbjct: 309 WFGLISKLWPNAKFISSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLP 368

Query: 366 PENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQYELV 425
           PE+V+FAV+PTFSYFEFIPL+R+  Q D     + +  DF+E +P+PLSQVK+GQ+YELV
Sbjct: 369 PEDVSFAVIPTFSYFEFIPLYRRQNQSD-----ICIDGDFVEDKPVPLSQVKLGQEYELV 428

Query: 426 LTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQL 485
           LTTFTGLYR RLGDVVEV  FH  TPKL+FI RRKL+LT+NIDKNTEKDLQ VV++ SQL
Sbjct: 429 LTTFTGLYRYRLGDVVEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQL 488

Query: 486 LADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSR 545
           L+  + AE+VDFTSHAD+  +PGHYVI+WE++G  +D  L  CC EMD AFVDYGYVVSR
Sbjct: 489 LSRSTRAEVVDFTSHADVIARPGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSR 548

Query: 546 RSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHS 605
           R N+IGPLELR+VERG+F K+ E  +G    L+QFKTPRCTTN V+L ILN ST+K F S
Sbjct: 549 RMNSIGPLELRVVERGTFGKVAERCVGKCGGLNQFKTPRCTTNSVMLDILNDSTIKRFRS 590

Query: 606 TAY 608
           +AY
Sbjct: 609 SAY 590

[Close]

BLAST of Carg03169 vs. TAIR 10
Match: AT2G46370.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 538.1 bits (1385), Expect = 9.1e-153
Identity = 276/614 (44.95%), Postives = 387/614 (63.03%), Query Frame = 0


Query: 1   MFEEVED-----MVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDD 60
           M E+VE      ++D F+ ++  A   Q +TL+EIL +N    YL       N     + 
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQ------NCGLNGNA 60

Query: 61  FALESLFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFT 120
              E  F S++PL +  + EP+++R+ DGD+SP+LT  P+  +SLSSGT++GR K++PFT
Sbjct: 61  TDPEEAFKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFT 120

Query: 121 PHSAQTTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGG 180
               + TL +FR A A+R+               R +P  + G+ L+FI+SSKQ  + GG
Sbjct: 121 DELMENTLQLFRTAFAFRN---------------RDFPIDDNGKALQFIFSSKQYISTGG 180

Query: 181 ITTGTATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFV 240
           +  GTATT+ Y +  FK       S +CSP EVI   D  Q+ YCHLL G+ F DQV++V
Sbjct: 181 VPVGTATTNVYRNPNFKAGMKSITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYV 240

Query: 241 TSTFAYTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQA 300
            + FA+ +V AF   E  W +I  DI    L +RIT+P +R A+  +++PNP L   I+ 
Sbjct: 241 FAVFAHGLVHAFRTFEQVWEEIVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRT 300

Query: 301 VCEELERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWI 360
            C  L   +W  L+P L+PN KYVY IMTGSM+PY+ KLR YAG LPLVS DYGS+E WI
Sbjct: 301 KCMSL--SNWYGLIPALFPNAKYVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWI 360

Query: 361 GVNVDPCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQV 420
             NV P L PE  TFAV+P   YFEF+P+       + G           E +P+ L+QV
Sbjct: 361 AANVTPRLSPEEATFAVIPNLGYFEFLPV------SETGEG---------EEKPVGLTQV 420

Query: 421 KIGQQYELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQ 480
           KIG++YE+V+T + GLYR RLGDVV+V GF+N TP+L FICRR L+L++NIDKNTE+DLQ
Sbjct: 421 KIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQ 480

Query: 481 LVVERGSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAF 540
           L VE  ++ L+++   E++DF+S+ D++  PGHY IFWE+ G   + VL  CC+ +D AF
Sbjct: 481 LSVESAAKRLSEEK-IEVIDFSSYIDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAF 540

Query: 541 VDYGYVVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTI 600
           +D GYV SR+  TIG LELR+V +G+F KI EH++G G++  QFK PRC   +N  +L I
Sbjct: 541 IDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQI 575

Query: 601 LNLSTVKAFHSTAY 608
           L  + V ++ STA+
Sbjct: 601 LCENVVSSYFSTAF 575

[Close]

BLAST of Carg03169 vs. TAIR 10
Match: AT2G46370.2 (Auxin-responsive GH3 family protein )

HSP 1 Score: 538.1 bits (1385), Expect = 9.1e-153
Identity = 276/614 (44.95%), Postives = 387/614 (63.03%), Query Frame = 0


Query: 1   MFEEVED-----MVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDD 60
           M E+VE      ++D F+ ++  A   Q +TL+EIL +N    YL       N     + 
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQ------NCGLNGNA 60

Query: 61  FALESLFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFT 120
              E  F S++PL +  + EP+++R+ DGD+SP+LT  P+  +SLSSGT++GR K++PFT
Sbjct: 61  TDPEEAFKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFT 120

Query: 121 PHSAQTTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGG 180
               + TL +FR A A+R+               R +P  + G+ L+FI+SSKQ  + GG
Sbjct: 121 DELMENTLQLFRTAFAFRN---------------RDFPIDDNGKALQFIFSSKQYISTGG 180

Query: 181 ITTGTATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFV 240
           +  GTATT+ Y +  FK       S +CSP EVI   D  Q+ YCHLL G+ F DQV++V
Sbjct: 181 VPVGTATTNVYRNPNFKAGMKSITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYV 240

Query: 241 TSTFAYTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQA 300
            + FA+ +V AF   E  W +I  DI    L +RIT+P +R A+  +++PNP L   I+ 
Sbjct: 241 FAVFAHGLVHAFRTFEQVWEEIVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRT 300

Query: 301 VCEELERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWI 360
            C  L   +W  L+P L+PN KYVY IMTGSM+PY+ KLR YAG LPLVS DYGS+E WI
Sbjct: 301 KCMSL--SNWYGLIPALFPNAKYVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWI 360

Query: 361 GVNVDPCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQV 420
             NV P L PE  TFAV+P   YFEF+P+       + G           E +P+ L+QV
Sbjct: 361 AANVTPRLSPEEATFAVIPNLGYFEFLPV------SETGEG---------EEKPVGLTQV 420

Query: 421 KIGQQYELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQ 480
           KIG++YE+V+T + GLYR RLGDVV+V GF+N TP+L FICRR L+L++NIDKNTE+DLQ
Sbjct: 421 KIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQ 480

Query: 481 LVVERGSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAF 540
           L VE  ++ L+++   E++DF+S+ D++  PGHY IFWE+ G   + VL  CC+ +D AF
Sbjct: 481 LSVESAAKRLSEEK-IEVIDFSSYIDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAF 540

Query: 541 VDYGYVVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTI 600
           +D GYV SR+  TIG LELR+V +G+F KI EH++G G++  QFK PRC   +N  +L I
Sbjct: 541 IDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQI 575

Query: 601 LNLSTVKAFHSTAY 608
           L  + V ++ STA+
Sbjct: 601 LCENVVSSYFSTAF 575

[Close]

BLAST of Carg03169 vs. TAIR 10
Match: AT2G46370.4 (Auxin-responsive GH3 family protein )

HSP 1 Score: 538.1 bits (1385), Expect = 9.1e-153
Identity = 276/614 (44.95%), Postives = 387/614 (63.03%), Query Frame = 0


Query: 1   MFEEVED-----MVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDD 60
           M E+VE      ++D F+ ++  A   Q +TL+EIL +N    YL       N     + 
Sbjct: 12  MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQ------NCGLNGNA 71

Query: 61  FALESLFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFT 120
              E  F S++PL +  + EP+++R+ DGD+SP+LT  P+  +SLSSGT++GR K++PFT
Sbjct: 72  TDPEEAFKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFT 131

Query: 121 PHSAQTTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGG 180
               + TL +FR A A+R+               R +P  + G+ L+FI+SSKQ  + GG
Sbjct: 132 DELMENTLQLFRTAFAFRN---------------RDFPIDDNGKALQFIFSSKQYISTGG 191

Query: 181 ITTGTATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFV 240
           +  GTATT+ Y +  FK       S +CSP EVI   D  Q+ YCHLL G+ F DQV++V
Sbjct: 192 VPVGTATTNVYRNPNFKAGMKSITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYV 251

Query: 241 TSTFAYTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQA 300
            + FA+ +V AF   E  W +I  DI    L +RIT+P +R A+  +++PNP L   I+ 
Sbjct: 252 FAVFAHGLVHAFRTFEQVWEEIVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRT 311

Query: 301 VCEELERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWI 360
            C  L   +W  L+P L+PN KYVY IMTGSM+PY+ KLR YAG LPLVS DYGS+E WI
Sbjct: 312 KCMSL--SNWYGLIPALFPNAKYVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWI 371

Query: 361 GVNVDPCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQV 420
             NV P L PE  TFAV+P   YFEF+P+       + G           E +P+ L+QV
Sbjct: 372 AANVTPRLSPEEATFAVIPNLGYFEFLPV------SETGEG---------EEKPVGLTQV 431

Query: 421 KIGQQYELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQ 480
           KIG++YE+V+T + GLYR RLGDVV+V GF+N TP+L FICRR L+L++NIDKNTE+DLQ
Sbjct: 432 KIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQ 491

Query: 481 LVVERGSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAF 540
           L VE  ++ L+++   E++DF+S+ D++  PGHY IFWE+ G   + VL  CC+ +D AF
Sbjct: 492 LSVESAAKRLSEEK-IEVIDFSSYIDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAF 551

Query: 541 VDYGYVVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTI 600
           +D GYV SR+  TIG LELR+V +G+F KI EH++G G++  QFK PRC   +N  +L I
Sbjct: 552 IDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQI 586

Query: 601 LNLSTVKAFHSTAY 608
           L  + V ++ STA+
Sbjct: 612 LCENVVSSYFSTAF 586

[Close]

BLAST of Carg03169 vs. TAIR 10
Match: AT2G46370.3 (Auxin-responsive GH3 family protein )

HSP 1 Score: 506.5 bits (1303), Expect = 2.9e-143
Identity = 251/529 (47.45%), Postives = 345/529 (65.22%), Query Frame = 0


Query: 81  IADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLIFRIAAAYRSRYQCNM 140
           + DGD+SP+LT  P+  +SLSSGT++GR K++PFT    + TL +FR A A+R+      
Sbjct: 1   MVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLFRTAFAFRN------ 60

Query: 141 TLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 200
                    R +P  + G+ L+FI+SSKQ  + GG+  GTATT+ Y +  FK       S
Sbjct: 61  ---------RDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITS 120

Query: 201 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 260
            +CSP EVI   D  Q+ YCHLL G+ F DQV++V + FA+ +V AF   E  W +I  D
Sbjct: 121 PSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTD 180

Query: 261 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWPNCKYVY 320
           I    L +RIT+P +R A+  +++PNP L   I+  C  L   +W  L+P L+PN KYVY
Sbjct: 181 IKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIPALFPNAKYVY 240

Query: 321 SIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYFE 380
            IMTGSM+PY+ KLR YAG LPLVS DYGS+E WI  NV P L PE  TFAV+P   YFE
Sbjct: 241 GIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFE 300

Query: 381 FIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGDVV 440
           F+P+       + G           E +P+ L+QVKIG++YE+V+T + GLYR RLGDVV
Sbjct: 301 FLPV------SETGEG---------EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVV 360

Query: 441 EVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSHA 500
           +V GF+N TP+L FICRR L+L++NIDKNTE+DLQL VE  ++ L+++   E++DF+S+ 
Sbjct: 361 KVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK-IEVIDFSSYI 420

Query: 501 DLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVERG 560
           D++  PGHY IFWE+ G   + VL  CC+ +D AF+D GYV SR+  TIG LELR+V +G
Sbjct: 421 DVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKG 480

Query: 561 SFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAY 608
           +F KI EH++G G++  QFK PRC   +N  +L IL  + V ++ STA+
Sbjct: 481 TFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 496

The following BLAST results are available for this feature:

BLAST of Carg03169 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7010696.1	0.0e+00	100.00	Indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita argyrosperma subsp. argy...	[more]
KAG6570848.1	0.0e+00	97.20	Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_023512179.1	0.0e+00	96.88	indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita pepo subsp. pepo]	[more]
XP_022943441.1	0.0e+00	96.88	indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita moschata]	[more]
XP_022986272.1	0.0e+00	96.05	indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita maxima]	[more]

BLAST of Carg03169 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZNS2	7.8e-234	65.67	Indole-3-acetic acid-amido synthetase GH3.10 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
A0A1J6KGJ9	3.1e-166	48.68	Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 ...	[more]
A0A314KSQ4	7.7e-165	49.17	Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 ...	[more]
Q6I581	1.3e-159	47.36	Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=3994...	[more]
Q9SKE2	1.3e-151	44.95	Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 ...	[more]

BLAST of Carg03169 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FRQ1	0.0e+00	96.88	indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1J741	0.0e+00	96.05	indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A5D3B9T4	1.6e-290	81.49	Jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S3BIL6	1.6e-290	81.49	jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A5A7VB01	8.3e-287	80.99	Jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo var. makuwa OX=11...	[more]

BLAST of Carg03169 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G03400.1	5.6e-235	65.67	Auxin-responsive GH3 family protein	[more]
AT2G46370.1	9.1e-153	44.95	Auxin-responsive GH3 family protein	[more]
AT2G46370.2	9.1e-153	44.95	Auxin-responsive GH3 family protein	[more]
AT2G46370.4	9.1e-153	44.95	Auxin-responsive GH3 family protein	[more]
AT2G46370.3	2.9e-143	47.45	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 11..587 e-value: 3.6E-173 score: 577.2
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 6..607
None	No IPR available	PANTHER	PTHR31901:SF48	INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.10	coord: 6..607

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg03169-RA	Carg03169-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009416	response to light stimulus
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016881	acid-amino acid ligase activity