Carg03143 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg03143
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	APO protein 4, mitochondrial-like
Location	Carg_Chr20: 2885267 .. 2887895 (-)
RNA-Seq Expression	Carg03143
Synteny	Carg03143

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCGTTTCTGTGGCGCCCTCGTTTTCCCTCTGGACAAGTGGAACTGCAAATCTTCATTTGATCTTACAATTCTGCAAATTAATTCTATGGAAATGCCTGTTCTTCCTCCTGTCTGAGCAAATCTGGCTTCAATGCTCCGTCGGCGTCGCTGTTAGTTGTTTCATCTTGTCTTTCAAGCGCTGTGTCTCTCTGCGGTCTTCTCTCGAAATCTCATCCTTCGTTCTTTGAACTCTCAATCTTACTCGCAGTCCGAGCACTTTCGCCGTTGAATTCGAAGGCTCATGTGATTGGATTAAGATCTTAATTTATTCCTTCAAGCTTGTCGCTGGTCGTTTTCCTAAGATTTGGTAAATGTTAGCGCTCGCACCATGAAAACTATGCCTTTTATGGCTCTTAGAAGGAAGTTCTGTGAGAATATTGTGCAGGAATTCGTGCTACATCGATGCTACAGTTCTAAAGTCGACTTGAAGAAGCTTCGTCCAATGATTTTGAAGAGAATTCAAAATCGGGCTAATAACTGTCCTATAAAAGGTCTAATTCCAGTGGCACAGCAAGTCTTTGAAGCTAGGGCAATGCTTATTCATGGAGTTTCCACTCTTCTCAAAGCATTTCCGGTTGTCTCTTGCAAGTCAGTGTCCCTTGAACAAACTATCTTTAATTTCTCATTTTTGTGTTATGATTTTACTCTGTTTCGTTTTCTTTTACTTAGTTTTTATCCGAAGTTGCATGTCCCTTGAGCTAGTATGTGTAGTAGGGATGTACCGTTTTGAAATGCTGATTGGCCTTGCTTATCATCTAGCTATTACCTGACCATTCCTGCGCAGGTAGTGAGTTCAAAAAATTCATCAATGCATATTATCTAGTTCTTTATGCACAATTGGGCCGTCTGTGAAGTCTGTTCTTTCTGAAATTTTGATTGAACAATCCTGAGGTATTAATATGAATGCATGTTTGTGAATGCTGCTTCTAAAATTCGTAGATTACTGAAGGTGCCCGGAATTCATGAATTCTCTGGTAAAGGGGAGTCGAGGTTGAATTCCTTGGGCCCTTTGTATACACCGCCCGTCGCTCCTACCAATTGAATGGTCATGTGAAGTGTTCGGATTGTGATGACGTGAGCGGTTCGCTGCCTGCGACGTCGCGAGAAGTCCACTGAACTTTATCATTTAGAGGAAGGAGAAGTTGTAACAAGGTTTCCATAGGGGAACCTGCAGAAGGATCATTGTCGATGCCTAAACATCAAACGACCCGTGAACACGTTTAAGTCTGTTCTTTTTGAAGATTATATTAAAAACTCTCAGCTGTTAACGTGAATACATGTTTGTGAAGGCTGTTTCTGAAACTTGTAGGATCATTTTGTTGTTTTTATAGATTTTGTCCAGAAGTATATGTTGGTGAGAAAGGTCATCTGATACGAAGCTGCGGCGGTTACAAACGCGGGCCTAAAAATCAGGTTCATGAGTGGATCAGAGGTGGTTTGAATGATGTCCTTGTCTCAGTAGAAGCATTTCACCGCCATCACATGTTCGAAAAGGTTAATGAACATGATGAGAGGCTCAATTTTGAGCGTGTTCCAGCAGTTGTGGAACTCTGCTGGCAAGCTGGAGCCAATACAAATGATGAAAATCTGTCTTCAAGCACTTGGAATTCAGTTGGTGGTGGTGGTGGTGGTGGTGGTGGAGGCTCAGGCAGGGATGAACCTTTATCCGGTAATGAGATGAGGCTTTTGGCAACCGAAACTCTCAGGGCGTGGGAAACGGTTCGAACGGGCGTGCAGAAACTGTTAATGGTATATCCTACTAAGATCTGTAAATACTGCTCAGACGTTCATGTTGGGCCATCAGTACACCAAGCTAGACCATGTGGAGTTTTCAAATGTGAGAGTTGGCGAGGATCCCACTTCTGGGAGAAGGCGGATGTAGATGATTTAGTTCCTCTGAAGATCGTATGGCATCGGCGACCTCAAGATCCTCCCGTGCTCGTTGATGAAGGGAGGGATTATTACGGGCAATCGCCAGCAGTCCTGGCTCTTTGCACACAGGCTGGTGCCATTGCACCACCTAAGTATCATTGTATGATGAAAGTTCAAGGCTTACCACCACCTCAGAGTCACCTCAAGCTATGAAATCTTCTTTTGTTCGACTTCACGTGGAGCTTGACAACAACACTCGACTCGATTACAGTTCACGACCTTCAGGTGACCGTGCCTATTTATTCTCTTTCTTCCTGCTTTATCTGTTTGGTTGATCTAGTAAACATATAGAAAAAGTAACATGATGAACATCAAGCATAGCAACTTGGGAAAAGTTATCAAAAAGTTGAAGAATTTTGAACTTGAGAATGTCTTGTAGATTCGTCTTCATTCTTTTCAGTAGAATAACATGTCACGGGAGGCACTTTTATAGGAAACTTGTATGTAAATTTTATGGATTTAAGGAATTGCATGTAGCTATTCAAAATCAAGACACCATTATGAACATGGGTCAGGTCGGTCAACCTTTGAACTAAACGTTTTGGGTATGCAAACTCCTAGTTCACAAATATTAAAACCTCGAAATCAATTTGATTTCGTTTCAAATGATTGCAACTCTGGAGCATTTGATCTAATTATCTTGTTGAAACATAAGACT

mRNA sequence

TCGTTTCTGTGGCGCCCTCGTTTTCCCTCTGGACAAGTGGAACTGCAAATCTTCATTTGATCTTACAATTCTGCAAATTAATTCTATGGAAATGCCTGTTCTTCCTCCTGTCTGAGCAAATCTGGCTTCAATGCTCCGTCGGCGTCGCTGTTAGTTGTTTCATCTTGTCTTTCAAGCGCTGTGTCTCTCTGCGGTCTTCTCTCGAAATCTCATCCTTCGTTCTTTGAACTCTCAATCTTACTCGCAGTCCGAGCACTTTCGCCGTTGAATTCGAAGGCTCATGTGATTGGATTAAGATCTTAATTTATTCCTTCAAGCTTGTCGCTGGTCGTTTTCCTAAGATTTGGTAAATGTTAGCGCTCGCACCATGAAAACTATGCCTTTTATGGCTCTTAGAAGGAAGTTCTGTGAGAATATTGTGCAGGAATTCGTGCTACATCGATGCTACAGTTCTAAAGTCGACTTGAAGAAGCTTCGTCCAATGATTTTGAAGAGAATTCAAAATCGGGCTAATAACTGTCCTATAAAAGGTCTAATTCCAGTGGCACAGCAAGTCTTTGAAGCTAGGGCAATGCTTATTCATGGAGTTTCCACTCTTCTCAAAGCATTTCCGGTTGTCTCTTGCAAATTTTGTCCAGAAGTATATGTTGGTGAGAAAGGTCATCTGATACGAAGCTGCGGCGGTTACAAACGCGGGCCTAAAAATCAGGTTCATGAGTGGATCAGAGGTGGTTTGAATGATGTCCTTGTCTCAGTAGAAGCATTTCACCGCCATCACATGTTCGAAAAGGTTAATGAACATGATGAGAGGCTCAATTTTGAGCGTGTTCCAGCAGTTGTGGAACTCTGCTGGCAAGCTGGAGCCAATACAAATGATGAAAATCTGTCTTCAAGCACTTGGAATTCAGTTGGTGGTGGTGGTGGTGGTGGTGGTGGAGGCTCAGGCAGGGATGAACCTTTATCCGGTAATGAGATGAGGCTTTTGGCAACCGAAACTCTCAGGGCGTGGGAAACGGTTCGAACGGGCGTGCAGAAACTGTTAATGGTATATCCTACTAAGATCTGTAAATACTGCTCAGACGTTCATGTTGGGCCATCAGTACACCAAGCTAGACCATGTGGAGTTTTCAAATGTGAGAGTTGGCGAGGATCCCACTTCTGGGAGAAGGCGGATGTAGATGATTTAGTTCCTCTGAAGATCGTATGGCATCGGCGACCTCAAGATCCTCCCGTGCTCGTTGATGAAGGGAGGGATTATTACGGGCAATCGCCAGCAGTCCTGGCTCTTTGCACACAGGCTGGTGCCATTGCACCACCTAAGTATCATTGTATGATGAAAGTTCAAGGCTTACCACCACCTCAGAGTCACCTCAAGCTATGAAATCTTCTTTTGTTCGACTTCACGTGGAGCTTGACAACAACACTCGACTCGATTACAGTTCACGACCTTCAGGTGACCGTGCCTATTTATTCTCTTTCTTCCTGCTTTATCTGTTTGGTTGATCTAGTAAACATATAGAAAAAGTAACATGATGAACATCAAGCATAGCAACTTGGGAAAAGTTATCAAAAAGTTGAAGAATTTTGAACTTGAGAATGTCTTGTAGATTCGTCTTCATTCTTTTCAGTAGAATAACATGTCACGGGAGGCACTTTTATAGGAAACTTGTATGTAAATTTTATGGATTTAAGGAATTGCATGTAGCTATTCAAAATCAAGACACCATTATGAACATGGGTCAGGTCGGTCAACCTTTGAACTAAACGTTTTGGGTATGCAAACTCCTAGTTCACAAATATTAAAACCTCGAAATCAATTTGATTTCGTTTCAAATGATTGCAACTCTGGAGCATTTGATCTAATTATCTTGTTGAAACATAAGACT

Coding sequence (CDS)

ATGAAAACTATGCCTTTTATGGCTCTTAGAAGGAAGTTCTGTGAGAATATTGTGCAGGAATTCGTGCTACATCGATGCTACAGTTCTAAAGTCGACTTGAAGAAGCTTCGTCCAATGATTTTGAAGAGAATTCAAAATCGGGCTAATAACTGTCCTATAAAAGGTCTAATTCCAGTGGCACAGCAAGTCTTTGAAGCTAGGGCAATGCTTATTCATGGAGTTTCCACTCTTCTCAAAGCATTTCCGGTTGTCTCTTGCAAATTTTGTCCAGAAGTATATGTTGGTGAGAAAGGTCATCTGATACGAAGCTGCGGCGGTTACAAACGCGGGCCTAAAAATCAGGTTCATGAGTGGATCAGAGGTGGTTTGAATGATGTCCTTGTCTCAGTAGAAGCATTTCACCGCCATCACATGTTCGAAAAGGTTAATGAACATGATGAGAGGCTCAATTTTGAGCGTGTTCCAGCAGTTGTGGAACTCTGCTGGCAAGCTGGAGCCAATACAAATGATGAAAATCTGTCTTCAAGCACTTGGAATTCAGTTGGTGGTGGTGGTGGTGGTGGTGGTGGAGGCTCAGGCAGGGATGAACCTTTATCCGGTAATGAGATGAGGCTTTTGGCAACCGAAACTCTCAGGGCGTGGGAAACGGTTCGAACGGGCGTGCAGAAACTGTTAATGGTATATCCTACTAAGATCTGTAAATACTGCTCAGACGTTCATGTTGGGCCATCAGTACACCAAGCTAGACCATGTGGAGTTTTCAAATGTGAGAGTTGGCGAGGATCCCACTTCTGGGAGAAGGCGGATGTAGATGATTTAGTTCCTCTGAAGATCGTATGGCATCGGCGACCTCAAGATCCTCCCGTGCTCGTTGATGAAGGGAGGGATTATTACGGGCAATCGCCAGCAGTCCTGGCTCTTTGCACACAGGCTGGTGCCATTGCACCACCTAAGTATCATTGTATGATGAAAGTTCAAGGCTTACCACCACCTCAGAGTCACCTCAAGCTATGA

Protein sequence

MKTMPFMALRRKFCENIVQEFVLHRCYSSKVDLKKLRPMILKRIQNRANNCPIKGLIPVAQQVFEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEWIRGGLNDVLVSVEAFHRHHMFEKVNEHDERLNFERVPAVVELCWQAGANTNDENLSSSTWNSVGGGGGGGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYPTKICKYCSDVHVGPSVHQARPCGVFKCESWRGSHFWEKADVDDLVPLKIVWHRRPQDPPVLVDEGRDYYGQSPAVLALCTQAGAIAPPKYHCMMKVQGLPPPQSHLKL

Homology

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BLAST of Carg03143 vs. NCBI nr
Match: KAG7010722.1 (APO protein 4, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 708.4 bits (1827), Expect = 3.0e-200
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0


Query: 1   MKTMPFMALRRKFCENIVQEFVLHRCYSSKVDLKKLRPMILKRIQNRANNCPIKGLIPVA 60
           MKTMPFMALRRKFCENIVQEFVLHRCYSSKVDLKKLRPMILKRIQNRANNCPIKGLIPVA
Sbjct: 1   MKTMPFMALRRKFCENIVQEFVLHRCYSSKVDLKKLRPMILKRIQNRANNCPIKGLIPVA 60

Query: 61  QQVFEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEWIR 120
           QQVFEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEWIR
Sbjct: 61  QQVFEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEWIR 120

Query: 121 GGLNDVLVSVEAFHRHHMFEKVNEHDERLNFERVPAVVELCWQAGANTNDENLSSSTWNS 180
           GGLNDVLVSVEAFHRHHMFEKVNEHDERLNFERVPAVVELCWQAGANTNDENLSSSTWNS
Sbjct: 121 GGLNDVLVSVEAFHRHHMFEKVNEHDERLNFERVPAVVELCWQAGANTNDENLSSSTWNS 180

Query: 181 VGGGGGGGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYPTKICKYCSDVH 240
           VGGGGGGGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYPTKICKYCSDVH
Sbjct: 181 VGGGGGGGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYPTKICKYCSDVH 240

Query: 241 VGPSVHQARPCGVFKCESWRGSHFWEKADVDDLVPLKIVWHRRPQDPPVLVDEGRDYYGQ 300
           VGPSVHQARPCGVFKCESWRGSHFWEKADVDDLVPLKIVWHRRPQDPPVLVDEGRDYYGQ
Sbjct: 241 VGPSVHQARPCGVFKCESWRGSHFWEKADVDDLVPLKIVWHRRPQDPPVLVDEGRDYYGQ 300

Query: 301 SPAVLALCTQAGAIAPPKYHCMMKVQGLPPPQSHLKL 338
           SPAVLALCTQAGAIAPPKYHCMMKVQGLPPPQSHLKL
Sbjct: 301 SPAVLALCTQAGAIAPPKYHCMMKVQGLPPPQSHLKL 337

[Close]

BLAST of Carg03143 vs. NCBI nr
Match: KAG6570875.1 (APO protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 698.0 bits (1800), Expect = 4.1e-197
Identity = 334/335 (99.70%), Postives = 334/335 (99.70%), Query Frame = 0


Query: 4   MPFMALRRKFCENIVQEFVLHRCYSSKVDLKKLRPMILKRIQNRANNCPIKGLIPVAQQV 63
           MPFMALRRKFCENIVQEFVLHRCYSSKVDLKKLRPMILKRIQNRANNCPIKGLIPVAQQV
Sbjct: 1   MPFMALRRKFCENIVQEFVLHRCYSSKVDLKKLRPMILKRIQNRANNCPIKGLIPVAQQV 60

Query: 64  FEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEWIRGGL 123
           FEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEWIRGGL
Sbjct: 61  FEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEWIRGGL 120

Query: 124 NDVLVSVEAFHRHHMFEKVNEHDERLNFERVPAVVELCWQAGANTNDENLSSSTWNSV-G 183
           NDVLVSVEAFHRHHMFEKVNEHDERLNFERVPAVVELCWQAGANTNDENLSSSTWNSV G
Sbjct: 121 NDVLVSVEAFHRHHMFEKVNEHDERLNFERVPAVVELCWQAGANTNDENLSSSTWNSVGG 180

Query: 184 GGGGGGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYPTKICKYCSDVHVG 243
           GGGGGGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYPTKICKYCSDVHVG
Sbjct: 181 GGGGGGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYPTKICKYCSDVHVG 240

Query: 244 PSVHQARPCGVFKCESWRGSHFWEKADVDDLVPLKIVWHRRPQDPPVLVDEGRDYYGQSP 303
           PSVHQARPCGVFKCESWRGSHFWEKADVDDLVPLKIVWHRRPQDPPVLVDEGRDYYGQSP
Sbjct: 241 PSVHQARPCGVFKCESWRGSHFWEKADVDDLVPLKIVWHRRPQDPPVLVDEGRDYYGQSP 300

Query: 304 AVLALCTQAGAIAPPKYHCMMKVQGLPPPQSHLKL 338
           AVLALCTQAGAIAPPKYHCMMKVQGLPPPQSHLKL
Sbjct: 301 AVLALCTQAGAIAPPKYHCMMKVQGLPPPQSHLKL 335

[Close]

BLAST of Carg03143 vs. NCBI nr
Match: XP_022943578.1 (APO protein 4, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 679.1 bits (1751), Expect = 2.0e-191
Identity = 324/337 (96.14%), Postives = 326/337 (96.74%), Query Frame = 0


Query: 1   MKTMPFMALRRKFCENIVQEFVLHRCYSSKVDLKKLRPMILKRIQNRANNCPIKGLIPVA 60
           MKTMPFMALRRKFCENIVQEFVLHRCYSSKVDLKKLRPM+LKRIQNRANNCPIKGLIPVA
Sbjct: 1   MKTMPFMALRRKFCENIVQEFVLHRCYSSKVDLKKLRPMVLKRIQNRANNCPIKGLIPVA 60

Query: 61  QQVFEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEWIR 120
           QQVFEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEW R
Sbjct: 61  QQVFEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEWTR 120

Query: 121 GGLNDVLVSVEAFHRHHMFEKVNEHDERLNFERVPAVVELCWQAGANTNDENLSSSTWNS 180
           GGLNDVLV VEAFHRHHMFEKVNEHDERLNFERVPAVVELCWQAGA+TNDEN SSSTWNS
Sbjct: 121 GGLNDVLVPVEAFHRHHMFEKVNEHDERLNFERVPAVVELCWQAGASTNDENPSSSTWNS 180

Query: 181 VGGGGGGGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYPTKICKYCSDVH 240
           V     GGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYPTKICKYCSDVH
Sbjct: 181 V-----GGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYPTKICKYCSDVH 240

Query: 241 VGPSVHQARPCGVFKCESWRGSHFWEKADVDDLVPLKIVWHRRPQDPPVLVDEGRDYYGQ 300
           VGPSVHQARPCGVFKCESWRGSHFWEKADVDDLVP KIVWHRRPQDPPVLVDEGRDYYGQ
Sbjct: 241 VGPSVHQARPCGVFKCESWRGSHFWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDYYGQ 300

Query: 301 SPAVLALCTQAGAIAPPKYHCMMKVQGLPPPQSHLKL 338
           SPAVLALCTQAG IAPPKYHC MKVQGLPPPQSHLKL
Sbjct: 301 SPAVLALCTQAGTIAPPKYHCRMKVQGLPPPQSHLKL 332

[Close]

BLAST of Carg03143 vs. NCBI nr
Match: XP_022986333.1 (APO protein 4, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 667.9 bits (1722), Expect = 4.5e-188
Identity = 320/337 (94.96%), Postives = 324/337 (96.14%), Query Frame = 0


Query: 1   MKTMPFMALRRKFCENIVQEFVLHRCYSSKVDLKKLRPMILKRIQNRANNCPIKGLIPVA 60
           MKTMPFMALRRKFCENIVQEF+LHRCYSSKVDLKKLRPMILKRIQNRANNCPIKGLIPVA
Sbjct: 1   MKTMPFMALRRKFCENIVQEFMLHRCYSSKVDLKKLRPMILKRIQNRANNCPIKGLIPVA 60

Query: 61  QQVFEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEWIR 120
           QQVFEARA+LIHGVSTLLK FPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEWIR
Sbjct: 61  QQVFEARAILIHGVSTLLKEFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEWIR 120

Query: 121 GGLNDVLVSVEAFHRHHMFEKVNEHDERLNFERVPAVVELCWQAGANTNDENLSSSTWNS 180
           GGLNDVLV VEAFHRHH+FEKVNEHDER  FERVPAVVELCWQAGANTNDENLSSSTWNS
Sbjct: 121 GGLNDVLVPVEAFHRHHIFEKVNEHDERFKFERVPAVVELCWQAGANTNDENLSSSTWNS 180

Query: 181 VGGGGGGGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYPTKICKYCSDVH 240
           V   GGGGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYP KIC YCSDVH
Sbjct: 181 V---GGGGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYPAKICNYCSDVH 240

Query: 241 VGPSVHQARPCGVFKCESWRGSHFWEKADVDDLVPLKIVWHRRPQDPPVLVDEGRDYYGQ 300
           VGPSVH+ARPCGVFKCESWRGSHFWEKADVDDLVP KIVWHRRPQDPPVLVDEGRDYYG 
Sbjct: 241 VGPSVHKARPCGVFKCESWRGSHFWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDYYGH 300

Query: 301 SPAVLALCTQAGAIAPPKYHCMMKVQGLPPPQSHLKL 338
           SPAVLALCT AGAIAPPKYHCMMKVQGL PPQSHLKL
Sbjct: 301 SPAVLALCTHAGAIAPPKYHCMMKVQGL-PPQSHLKL 333

[Close]

BLAST of Carg03143 vs. NCBI nr
Match: XP_038901944.1 (APO protein 4, mitochondrial [Benincasa hispida] >XP_038901945.1 APO protein 4, mitochondrial [Benincasa hispida] >XP_038901946.1 APO protein 4, mitochondrial [Benincasa hispida] >XP_038901947.1 APO protein 4, mitochondrial [Benincasa hispida])

HSP 1 Score: 570.9 bits (1470), Expect = 7.5e-159
Identity = 273/337 (81.01%), Postives = 299/337 (88.72%), Query Frame = 0


Query: 1   MKTMPFMALRRKFCENIVQEFVLHRCYSSKVDLKKLRPMILKRIQNRANNCPIKGLIPVA 60
           MKTM FMAL+RKF E +VQEFVLHRCYSSKV+LKKLRPMILKRIQNR  N PI+G+ PVA
Sbjct: 1   MKTMAFMALKRKFREIVVQEFVLHRCYSSKVNLKKLRPMILKRIQNREKNYPIRGMTPVA 60

Query: 61  QQVFEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEWIR 120
           QQV EAR MLIHGVSTLLK+FPV+SCKFCPEVYVGEKGHLIRSCGGYKRG KNQVHEWIR
Sbjct: 61  QQVLEARTMLIHGVSTLLKSFPVLSCKFCPEVYVGEKGHLIRSCGGYKRGAKNQVHEWIR 120

Query: 121 GGLNDVLVSVEAFHRHHMFEKVNEHDERLNFERVPAVVELCWQAGANTNDENLSSSTWNS 180
           G LND++V VE+FH HHMF+ V +HDER NFERVPAVVELCWQAGAN NDEN SSST NS
Sbjct: 121 GDLNDIIVPVESFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENQSSSTRNS 180

Query: 181 VGGGGGGGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYPTKICKYCSDVH 240
                 G GGGSGRD+PLSG+EM LLATET+RAWET+RTGVQKLLMVYP K+CKYCS+VH
Sbjct: 181 ------GEGGGSGRDKPLSGHEMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEVH 240

Query: 241 VGPSVHQARPCGVFKCESWRGSHFWEKADVDDLVPLKIVWHRRPQDPPVLVDEGRDYYGQ 300
           VGPS H+AR CGVFK E WRGSHFWEKAD+DDLVP K+VWHRRPQDPPVL+DEGRDYYG 
Sbjct: 241 VGPSGHKARLCGVFKYEHWRGSHFWEKADIDDLVPPKLVWHRRPQDPPVLIDEGRDYYGH 300

Query: 301 SPAVLALCTQAGAIAPPKYHCMMKVQGLPPPQSHLKL 338
           +PAVLALCTQAGA+AP KYHCMMKVQG+  P+S LKL
Sbjct: 301 TPAVLALCTQAGALAPSKYHCMMKVQGI-SPRSQLKL 330

[Close]

BLAST of Carg03143 vs. ExPASy Swiss-Prot
Match: Q9LSZ0 (APO protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO4 PE=2 SV=2)

HSP 1 Score: 344.4 bits (882), Expect = 1.5e-93
Identity = 162/298 (54.36%), Postives = 215/298 (72.15%), Query Frame = 0


Query: 32  DLKKLRPMILKRIQNRANNCPIKGLIPVAQQVFEARAMLIHGVSTLLKAFPVVSCKFCPE 91
           DL+KLRPMILKRI+NRA + P+K ++PVA+++  AR  LI  ++ LLK FPV++CKFC E
Sbjct: 34  DLRKLRPMILKRIENRAKDYPVKEIVPVAEEILIARKNLISNIAALLKVFPVLTCKFCSE 93

Query: 92  VYVGEKGHLIRSCGGYKRGPKNQVHEWIRGGLNDVLVSVEAFHRHHMFEKVNEHDERLNF 151
           V+VG++GHLI +C  Y R   N++HEW+ G +ND+LV VE++H H++ + V  H ER ++
Sbjct: 94  VFVGKEGHLIETCRSYIRRGNNRLHEWVPGSINDILVPVESYHLHNISQGVIRHQERFDY 153

Query: 152 ERVPAVVELCWQAGANTNDENLSSSTWNSVGGGGGGGGGGSGRD-EPLSGNEMRLLATET 211
           +RVPA++ELC QAGA   +E L  S  +            S  D   L   +++ +    
Sbjct: 154 DRVPAILELCCQAGAIHPEEILQYSEIHD-------NPQISEEDIRSLPAGDLKYVGANA 213

Query: 212 LRAWETVRTGVQKLLMVYPTKICKYCSDVHVGPSVHQARPCGVFKCESWRGSHFWEKADV 271
           L AWE VR GV+KLL+VYP+K+CK C +VHVGPS H+AR CGVFK ESWRG+H+WEKA V
Sbjct: 214 LMAWEKVRAGVKKLLLVYPSKVCKRCKEVHVGPSGHKARLCGVFKYESWRGTHYWEKAGV 273

Query: 272 DDLVPLKIVWHRRPQDPPVLVDEGRDYYGQSPAVLALCTQAGAIAPPKYHCMMKVQGL 329
           +DLVP K+VWHRRPQDP VLVDEGR YYG +PA+++LC+  GAI P KY C MK QGL
Sbjct: 274 NDLVPEKMVWHRRPQDPVVLVDEGRSYYGHAPAIVSLCSHTGAIVPVKYACKMKPQGL 324

[Close]

BLAST of Carg03143 vs. ExPASy Swiss-Prot
Match: Q8W4A5 (APO protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO2 PE=2 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 7.0e-51
Identity = 109/305 (35.74%), Postives = 159/305 (52.13%), Query Frame = 0


Query: 49  NNCPIKGLIPVAQQVFEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYK 108
           N   +K L+P+A +V+ AR  LI+ +  L+K   V +C +C E++VG  GH  +SC G  
Sbjct: 126 NGMVVKSLVPLAYKVYNARIRLINNLHRLMKVVRVNACGWCNEIHVGPYGHPFKSCKGPN 185

Query: 109 RGPKNQVHEWIRGGLNDVLVSVEAFHRHHMFEKVNEHDERLNFERVPAVVELCWQAGANT 168
              +  +HEW    + DV+V +EA+H      K   HDER +  RVPAVVELC Q G   
Sbjct: 186 TSQRKGLHEWTNSVIEDVIVPLEAYHLFDRLGKRIRHDERFSIPRVPAVVELCIQGGVEI 245

Query: 169 NDENLSSSTWNSVGGGGGGGGGGSGRD-----------------------EPLSGNEMRL 228
            +          +  G          +                        P S  E   
Sbjct: 246 PEFPAKRRRKPIIRIGKSEFVDADETELPDPEPQPPPVPLLTELPVSEITPPSSEEETVS 305

Query: 229 LATETLRAWETVRTGVQKLLMVYPTKICKYCSDVHVGPSVHQARPCGVFKCESWRGSHFW 288
           LA ETL+AWE +R G +KL+ +Y  ++C YC +VHVGP+ H+A+ CG FK +   G H W
Sbjct: 306 LAEETLQAWEEMRAGAKKLMRMYRVRVCGYCPEVHVGPTGHKAQNCGAFKHQQRNGQHGW 365

Query: 289 EKADVDDLVPLKIVWHRRPQDPPVLVDEGRDYYGQSPAVLALCTQAGAIAPPKYHCMMKV 330
           + A +DDL+P + VWH    + P +  E R +YGQ+PAV+ +C QAGA+ P  Y   M++
Sbjct: 366 QSAVLDDLIPPRYVWHVPDVNGPPMQRELRSFYGQAPAVVEICAQAGAVVPEHYRATMRL 425

[Close]

BLAST of Carg03143 vs. ExPASy Swiss-Prot
Match: Q9XIR4 (APO protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO1 PE=2 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 5.2e-46
Identity = 113/326 (34.66%), Postives = 163/326 (50.00%), Query Frame = 0


Query: 34  KKLRPM-ILKRIQNRANNCPIKGLIPVAQQVFEARAMLIHGVSTLLKAFPVVSCKFCPEV 93
           KKL  M I K++    N   +  L+PVA QV +   +LI G++ LL   PV +C  C  V
Sbjct: 103 KKLAQMGIEKQLDPPKNGLLVPNLVPVADQVIDNWKLLIKGLAQLLHVVPVFACSECGAV 162

Query: 94  YVGEKGHLIRSCGGYKRGPKNQVHEWIRGGLNDVLVSVEAFHRHHMFEKVNEHDERLNFE 153
           +V   GH IR C G     +   H W++G +NDVL+ VE++H +  F +  +H+ R  +E
Sbjct: 163 HVANVGHNIRDCNGPTNSQRRGSHSWVKGTINDVLIPVESYHMYDPFGRRIKHETRFEYE 222

Query: 154 RVPAVVELCWQAGANTNDENLSSSTW--NSVGGGGGGGGGGSGRDE-------------- 213
           R+PA+VELC QAG    +      T     +G      GG     E              
Sbjct: 223 RIPALVELCIQAGVEIPEYPCRRRTQPIRMMGKRVIDRGGYHKEPEKPQTSSSLSSPLAE 282

Query: 214 -----------PLSGNEMRLLATETLRAWETVRTGVQKLLMVYPTKICKYCSDVHVGPSV 273
                      P +  ++  +A ET+ A+E VR GV KL+  +  K C YCS+VHVGP  
Sbjct: 283 LDTLGVFERYPPPTPEDIPKIAQETMDAYEKVRLGVTKLMRKFTVKACGYCSEVHVGPWG 342

Query: 274 HQARPCGVFKCESWR-GSHFWEKADVDDLVPLKIVWHRRPQDPPVLVDEGRDYYGQSPAV 331
           H  + CG FK   WR G H W+ A VD++ P   VWH R      L    R +YG++PA+
Sbjct: 343 HSVKLCGEFK-HQWRDGKHGWQDALVDEVFPPNYVWHVRDLKGNPLTGNLRRFYGKAPAL 402

[Close]

BLAST of Carg03143 vs. ExPASy Swiss-Prot
Match: Q9FH50 (APO protein 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO3 PE=2 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 2.8e-39
Identity = 98/310 (31.61%), Postives = 155/310 (50.00%), Query Frame = 0


Query: 49  NNCPIKGLIPVAQQVFEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYK 108
           N   +  L+ VA  V   R ML+ G+S ++   PV  C+ C EV++G++GH IR+C G  
Sbjct: 91  NGLLVPELVDVAHCVHRCRNMLLSGLSKIIHHVPVHRCRLCAEVHIGKQGHEIRTCTGPG 150

Query: 109 RGPKNQVHEWIRGGLNDVLVSVEAFHRHHMFEKVNE-HDERLNFERVPAVVELCWQAGAN 168
            G ++  H W RG ++DV++  + FH +    K    HDER    ++ AV+ELC QAG +
Sbjct: 151 SGSRSATHVWKRGRVSDVVLFPKCFHLYDRAVKPRVIHDERFTVPKISAVLELCIQAGVD 210

Query: 169 TN---DENLSSSTWNSVGGGGGGGGGGSGRDE----------------PLSGNEMRLLAT 228
                 +  S   ++  G          G  E                      ++ L+ 
Sbjct: 211 LEKFPSKRRSKPVYSIEGRIVDFEDVNDGNSELAVTSTTTLIQEDDRCKEEKKSLKELSF 270

Query: 229 ETLRAWETVRTGVQKLLMVYPTKICKYCSDVHVGPSVHQARPCGVFKCESWRGSHFWEKA 288
           ET+ +W  +  GV+KL+  Y    C YC ++ VGP  H+ R C   K +   G H W++A
Sbjct: 271 ETMESWFEMVLGVRKLMERYRVWTCGYCPEIQVGPKGHKVRMCKATKHQMRDGMHAWQEA 330

Query: 289 DVDDLVPLKIVWH-RRPQDPPVLVDEGRDYYGQSPAVLALCTQAGAIAPPKYHCMMKVQG 338
            +DD+V    VWH R P D  VL +  + +YG++PAV+ +C Q GA  P +Y+ MM++  
Sbjct: 331 TIDDVVGPTYVWHVRDPTDGSVLDNSLKRFYGKAPAVIEMCVQGGAPVPDQYNSMMRLDV 390

[Close]

BLAST of Carg03143 vs. ExPASy TrEMBL
Match: A0A6J1FX59 (APO protein 4, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111448307 PE=4 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 9.5e-192
Identity = 324/337 (96.14%), Postives = 326/337 (96.74%), Query Frame = 0


Query: 1   MKTMPFMALRRKFCENIVQEFVLHRCYSSKVDLKKLRPMILKRIQNRANNCPIKGLIPVA 60
           MKTMPFMALRRKFCENIVQEFVLHRCYSSKVDLKKLRPM+LKRIQNRANNCPIKGLIPVA
Sbjct: 1   MKTMPFMALRRKFCENIVQEFVLHRCYSSKVDLKKLRPMVLKRIQNRANNCPIKGLIPVA 60

Query: 61  QQVFEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEWIR 120
           QQVFEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEW R
Sbjct: 61  QQVFEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEWTR 120

Query: 121 GGLNDVLVSVEAFHRHHMFEKVNEHDERLNFERVPAVVELCWQAGANTNDENLSSSTWNS 180
           GGLNDVLV VEAFHRHHMFEKVNEHDERLNFERVPAVVELCWQAGA+TNDEN SSSTWNS
Sbjct: 121 GGLNDVLVPVEAFHRHHMFEKVNEHDERLNFERVPAVVELCWQAGASTNDENPSSSTWNS 180

Query: 181 VGGGGGGGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYPTKICKYCSDVH 240
           V     GGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYPTKICKYCSDVH
Sbjct: 181 V-----GGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYPTKICKYCSDVH 240

Query: 241 VGPSVHQARPCGVFKCESWRGSHFWEKADVDDLVPLKIVWHRRPQDPPVLVDEGRDYYGQ 300
           VGPSVHQARPCGVFKCESWRGSHFWEKADVDDLVP KIVWHRRPQDPPVLVDEGRDYYGQ
Sbjct: 241 VGPSVHQARPCGVFKCESWRGSHFWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDYYGQ 300

Query: 301 SPAVLALCTQAGAIAPPKYHCMMKVQGLPPPQSHLKL 338
           SPAVLALCTQAG IAPPKYHC MKVQGLPPPQSHLKL
Sbjct: 301 SPAVLALCTQAGTIAPPKYHCRMKVQGLPPPQSHLKL 332

[Close]

BLAST of Carg03143 vs. ExPASy TrEMBL
Match: A0A6J1JG78 (APO protein 4, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111484108 PE=4 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 2.2e-188
Identity = 320/337 (94.96%), Postives = 324/337 (96.14%), Query Frame = 0


Query: 1   MKTMPFMALRRKFCENIVQEFVLHRCYSSKVDLKKLRPMILKRIQNRANNCPIKGLIPVA 60
           MKTMPFMALRRKFCENIVQEF+LHRCYSSKVDLKKLRPMILKRIQNRANNCPIKGLIPVA
Sbjct: 1   MKTMPFMALRRKFCENIVQEFMLHRCYSSKVDLKKLRPMILKRIQNRANNCPIKGLIPVA 60

Query: 61  QQVFEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEWIR 120
           QQVFEARA+LIHGVSTLLK FPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEWIR
Sbjct: 61  QQVFEARAILIHGVSTLLKEFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEWIR 120

Query: 121 GGLNDVLVSVEAFHRHHMFEKVNEHDERLNFERVPAVVELCWQAGANTNDENLSSSTWNS 180
           GGLNDVLV VEAFHRHH+FEKVNEHDER  FERVPAVVELCWQAGANTNDENLSSSTWNS
Sbjct: 121 GGLNDVLVPVEAFHRHHIFEKVNEHDERFKFERVPAVVELCWQAGANTNDENLSSSTWNS 180

Query: 181 VGGGGGGGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYPTKICKYCSDVH 240
           V   GGGGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYP KIC YCSDVH
Sbjct: 181 V---GGGGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYPAKICNYCSDVH 240

Query: 241 VGPSVHQARPCGVFKCESWRGSHFWEKADVDDLVPLKIVWHRRPQDPPVLVDEGRDYYGQ 300
           VGPSVH+ARPCGVFKCESWRGSHFWEKADVDDLVP KIVWHRRPQDPPVLVDEGRDYYG 
Sbjct: 241 VGPSVHKARPCGVFKCESWRGSHFWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDYYGH 300

Query: 301 SPAVLALCTQAGAIAPPKYHCMMKVQGLPPPQSHLKL 338
           SPAVLALCT AGAIAPPKYHCMMKVQGL PPQSHLKL
Sbjct: 301 SPAVLALCTHAGAIAPPKYHCMMKVQGL-PPQSHLKL 333

[Close]

BLAST of Carg03143 vs. ExPASy TrEMBL
Match: A0A6J1I7G7 (APO protein 4, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111469835 PE=4 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 3.3e-152
Identity = 261/325 (80.31%), Postives = 283/325 (87.08%), Query Frame = 0


Query: 4   MPFMALRRKFCENIVQEFVLHRCYSSKVDLKKLRPMILKRIQNRANNCPIKGLIPVAQQV 63
           M FMALRRK  EN+VQEF+L RCYSSKVDLKKLRPMILKR+QNRANN PIKG+IPVAQ+V
Sbjct: 1   MAFMALRRKLGENVVQEFMLQRCYSSKVDLKKLRPMILKRLQNRANNYPIKGMIPVAQEV 60

Query: 64  FEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEWIRGGL 123
            EARAMLIHGVSTLLK FPV+SCKFCPEVYVG+KGHLIRSCGGYKRG KN+VHEWIRG L
Sbjct: 61  LEARAMLIHGVSTLLKTFPVLSCKFCPEVYVGDKGHLIRSCGGYKRGAKNRVHEWIRGDL 120

Query: 124 NDVLVSVEAFHRHHMFEKVNEHDERLNFERVPAVVELCWQAGANTNDENLSSSTWNSVGG 183
           ND+LV VE+FH  HMF+ V +HDER +FERVPAVVELCWQAGAN NDENLSSSTWNS   
Sbjct: 121 NDILVPVESFHLRHMFQDVIKHDERFDFERVPAVVELCWQAGANPNDENLSSSTWNS--- 180

Query: 184 GGGGGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYPTKICKYCSDVHVGP 243
                  G GRDE LS +E+ +LA  T+RAWETVRTGVQKLLMVYPTK+CK+CS+VHVGP
Sbjct: 181 ------DGDGRDEHLSSHELMVLANSTIRAWETVRTGVQKLLMVYPTKVCKHCSEVHVGP 240

Query: 244 SVHQARPCGVFKCESWRGSHFWEKADVDDLVPLKIVWHRRPQDPPVLVDEGRDYYGQSPA 303
           S H+AR CGVFK ESWRGSHFWEK DVDDLVP K VWHRR QDPPVLVDEGRDYYG +PA
Sbjct: 241 SGHKARLCGVFKYESWRGSHFWEKGDVDDLVPPKSVWHRRAQDPPVLVDEGRDYYGHAPA 300

Query: 304 VLALCTQAGAIAPPKYHCMMKVQGL 329
           VLALC QAGAIAP KYHCMMKVQGL
Sbjct: 301 VLALCIQAGAIAPSKYHCMMKVQGL 316

[Close]

BLAST of Carg03143 vs. ExPASy TrEMBL
Match: A0A0A0LQG5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G421010 PE=4 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 3.3e-152
Identity = 263/330 (79.70%), Postives = 291/330 (88.18%), Query Frame = 0


Query: 1   MKTMPFMALRRKFCENIVQEFVLHRCYSSKVDLKKLRPMILKRIQNRANNCPIKGLIPVA 60
           MKTM FMA+RRKF +N+VQEF+L RCYSSKV+LKKLRPMILKRIQ+RA N PIKG+ PVA
Sbjct: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60

Query: 61  QQVFEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEWIR 120
           QQV EARAMLIHGVSTLLK+FPV+SCKFCPEVYVGE+GHLIRSCGGYKRG KNQVH+WIR
Sbjct: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIR 120

Query: 121 GGLNDVLVSVEAFHRHHMFEKVNEHDERLNFERVPAVVELCWQAGANTNDENLSSSTWNS 180
           G L D++V VEAFH HHMF+ V +HDER NFERVPAVVELC QAGAN +D+NL+SST NS
Sbjct: 121 GDLKDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNS 180

Query: 181 VGGGGGGGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYPTKICKYCSDVH 240
                   GGGSG DEPLS +EM LLATET+RAWET+RTGVQKLLMVYPTK+CKYCS+VH
Sbjct: 181 ------AEGGGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVH 240

Query: 241 VGPSVHQARPCGVFKCESWRGSHFWEKADVDDLVPLKIVWHRRPQDPPVLVDEGRDYYGQ 300
           VGPS H+AR CGVF  ESWRGSHFWEKADVDDLVP KIVWHRR QDPPVLVD+G+DYYG 
Sbjct: 241 VGPSGHKARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGH 300

Query: 301 SPAVLALCTQAGAIAPPKYHCMMKVQGLPP 331
           +PAV+ALCTQAG IAP KYHCMMKVQGL P
Sbjct: 301 APAVVALCTQAGVIAPFKYHCMMKVQGLSP 324

[Close]

BLAST of Carg03143 vs. ExPASy TrEMBL
Match: A0A5A7TJ18 (APO protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1639G00180 PE=4 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 5.6e-152
Identity = 262/330 (79.39%), Postives = 291/330 (88.18%), Query Frame = 0


Query: 1   MKTMPFMALRRKFCENIVQEFVLHRCYSSKVDLKKLRPMILKRIQNRANNCPIKGLIPVA 60
           MKTM FMA+RRKF +N+VQEF+L RCYSSKV+LKKLRPMILKRIQ+RA N PIKG+ PVA
Sbjct: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60

Query: 61  QQVFEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYKRGPKNQVHEWIR 120
           QQV EARAMLIHGVSTLLK+FPV+SCK+CPEVYVG +GHLIRSCGGYKRG KNQVH+WIR
Sbjct: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKYCPEVYVGAEGHLIRSCGGYKRGAKNQVHQWIR 120

Query: 121 GGLNDVLVSVEAFHRHHMFEKVNEHDERLNFERVPAVVELCWQAGANTNDENLSSSTWNS 180
           G LND++V VEAFH HHMF+ V +HDER NFERVPAVVELC QAG N +D++L+SST NS
Sbjct: 121 GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCCQAGVNPDDKDLASSTQNS 180

Query: 181 VGGGGGGGGGGSGRDEPLSGNEMRLLATETLRAWETVRTGVQKLLMVYPTKICKYCSDVH 240
                   GGGSG DEP S +EM LLATET+RAWET+RTGVQKLLMVYPTK+CKYCS+VH
Sbjct: 181 ------AEGGGSGMDEPFSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVH 240

Query: 241 VGPSVHQARPCGVFKCESWRGSHFWEKADVDDLVPLKIVWHRRPQDPPVLVDEGRDYYGQ 300
           VGPS H+AR CGVFK ESWRGSHFWEKADVDDLVP KIVWHRR QDPPVLVD+GRDYYG 
Sbjct: 241 VGPSGHKARLCGVFKYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGRDYYGH 300

Query: 301 SPAVLALCTQAGAIAPPKYHCMMKVQGLPP 331
           +PAV+ALCTQAGAIAP KYHCMMKVQGL P
Sbjct: 301 APAVVALCTQAGAIAPFKYHCMMKVQGLSP 324

[Close]

BLAST of Carg03143 vs. TAIR 10
Match: AT3G21740.1 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 344.4 bits (882), Expect = 1.1e-94
Identity = 162/298 (54.36%), Postives = 215/298 (72.15%), Query Frame = 0


Query: 32  DLKKLRPMILKRIQNRANNCPIKGLIPVAQQVFEARAMLIHGVSTLLKAFPVVSCKFCPE 91
           DL+KLRPMILKRI+NRA + P+K ++PVA+++  AR  LI  ++ LLK FPV++CKFC E
Sbjct: 34  DLRKLRPMILKRIENRAKDYPVKEIVPVAEEILIARKNLISNIAALLKVFPVLTCKFCSE 93

Query: 92  VYVGEKGHLIRSCGGYKRGPKNQVHEWIRGGLNDVLVSVEAFHRHHMFEKVNEHDERLNF 151
           V+VG++GHLI +C  Y R   N++HEW+ G +ND+LV VE++H H++ + V  H ER ++
Sbjct: 94  VFVGKEGHLIETCRSYIRRGNNRLHEWVPGSINDILVPVESYHLHNISQGVIRHQERFDY 153

Query: 152 ERVPAVVELCWQAGANTNDENLSSSTWNSVGGGGGGGGGGSGRD-EPLSGNEMRLLATET 211
           +RVPA++ELC QAGA   +E L  S  +            S  D   L   +++ +    
Sbjct: 154 DRVPAILELCCQAGAIHPEEILQYSEIHD-------NPQISEEDIRSLPAGDLKYVGANA 213

Query: 212 LRAWETVRTGVQKLLMVYPTKICKYCSDVHVGPSVHQARPCGVFKCESWRGSHFWEKADV 271
           L AWE VR GV+KLL+VYP+K+CK C +VHVGPS H+AR CGVFK ESWRG+H+WEKA V
Sbjct: 214 LMAWEKVRAGVKKLLLVYPSKVCKRCKEVHVGPSGHKARLCGVFKYESWRGTHYWEKAGV 273

Query: 272 DDLVPLKIVWHRRPQDPPVLVDEGRDYYGQSPAVLALCTQAGAIAPPKYHCMMKVQGL 329
           +DLVP K+VWHRRPQDP VLVDEGR YYG +PA+++LC+  GAI P KY C MK QGL
Sbjct: 274 NDLVPEKMVWHRRPQDPVVLVDEGRSYYGHAPAIVSLCSHTGAIVPVKYACKMKPQGL 324

[Close]

BLAST of Carg03143 vs. TAIR 10
Match: AT5G57930.1 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 202.6 bits (514), Expect = 5.0e-52
Identity = 109/305 (35.74%), Postives = 159/305 (52.13%), Query Frame = 0


Query: 49  NNCPIKGLIPVAQQVFEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYK 108
           N   +K L+P+A +V+ AR  LI+ +  L+K   V +C +C E++VG  GH  +SC G  
Sbjct: 126 NGMVVKSLVPLAYKVYNARIRLINNLHRLMKVVRVNACGWCNEIHVGPYGHPFKSCKGPN 185

Query: 109 RGPKNQVHEWIRGGLNDVLVSVEAFHRHHMFEKVNEHDERLNFERVPAVVELCWQAGANT 168
              +  +HEW    + DV+V +EA+H      K   HDER +  RVPAVVELC Q G   
Sbjct: 186 TSQRKGLHEWTNSVIEDVIVPLEAYHLFDRLGKRIRHDERFSIPRVPAVVELCIQGGVEI 245

Query: 169 NDENLSSSTWNSVGGGGGGGGGGSGRD-----------------------EPLSGNEMRL 228
            +          +  G          +                        P S  E   
Sbjct: 246 PEFPAKRRRKPIIRIGKSEFVDADETELPDPEPQPPPVPLLTELPVSEITPPSSEEETVS 305

Query: 229 LATETLRAWETVRTGVQKLLMVYPTKICKYCSDVHVGPSVHQARPCGVFKCESWRGSHFW 288
           LA ETL+AWE +R G +KL+ +Y  ++C YC +VHVGP+ H+A+ CG FK +   G H W
Sbjct: 306 LAEETLQAWEEMRAGAKKLMRMYRVRVCGYCPEVHVGPTGHKAQNCGAFKHQQRNGQHGW 365

Query: 289 EKADVDDLVPLKIVWHRRPQDPPVLVDEGRDYYGQSPAVLALCTQAGAIAPPKYHCMMKV 330
           + A +DDL+P + VWH    + P +  E R +YGQ+PAV+ +C QAGA+ P  Y   M++
Sbjct: 366 QSAVLDDLIPPRYVWHVPDVNGPPMQRELRSFYGQAPAVVEICAQAGAVVPEHYRATMRL 425

[Close]

BLAST of Carg03143 vs. TAIR 10
Match: AT5G57930.2 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 202.6 bits (514), Expect = 5.0e-52
Identity = 109/305 (35.74%), Postives = 159/305 (52.13%), Query Frame = 0


Query: 49  NNCPIKGLIPVAQQVFEARAMLIHGVSTLLKAFPVVSCKFCPEVYVGEKGHLIRSCGGYK 108
           N   +K L+P+A +V+ AR  LI+ +  L+K   V +C +C E++VG  GH  +SC G  
Sbjct: 129 NGMVVKSLVPLAYKVYNARIRLINNLHRLMKVVRVNACGWCNEIHVGPYGHPFKSCKGPN 188

Query: 109 RGPKNQVHEWIRGGLNDVLVSVEAFHRHHMFEKVNEHDERLNFERVPAVVELCWQAGANT 168
              +  +HEW    + DV+V +EA+H      K   HDER +  RVPAVVELC Q G   
Sbjct: 189 TSQRKGLHEWTNSVIEDVIVPLEAYHLFDRLGKRIRHDERFSIPRVPAVVELCIQGGVEI 248

Query: 169 NDENLSSSTWNSVGGGGGGGGGGSGRD-----------------------EPLSGNEMRL 228
            +          +  G          +                        P S  E   
Sbjct: 249 PEFPAKRRRKPIIRIGKSEFVDADETELPDPEPQPPPVPLLTELPVSEITPPSSEEETVS 308

Query: 229 LATETLRAWETVRTGVQKLLMVYPTKICKYCSDVHVGPSVHQARPCGVFKCESWRGSHFW 288
           LA ETL+AWE +R G +KL+ +Y  ++C YC +VHVGP+ H+A+ CG FK +   G H W
Sbjct: 309 LAEETLQAWEEMRAGAKKLMRMYRVRVCGYCPEVHVGPTGHKAQNCGAFKHQQRNGQHGW 368

Query: 289 EKADVDDLVPLKIVWHRRPQDPPVLVDEGRDYYGQSPAVLALCTQAGAIAPPKYHCMMKV 330
           + A +DDL+P + VWH    + P +  E R +YGQ+PAV+ +C QAGA+ P  Y   M++
Sbjct: 369 QSAVLDDLIPPRYVWHVPDVNGPPMQRELRSFYGQAPAVVEICAQAGAVVPEHYRATMRL 428

[Close]

BLAST of Carg03143 vs. TAIR 10
Match: AT1G64810.1 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 186.4 bits (472), Expect = 3.7e-47
Identity = 113/326 (34.66%), Postives = 163/326 (50.00%), Query Frame = 0


Query: 34  KKLRPM-ILKRIQNRANNCPIKGLIPVAQQVFEARAMLIHGVSTLLKAFPVVSCKFCPEV 93
           KKL  M I K++    N   +  L+PVA QV +   +LI G++ LL   PV +C  C  V
Sbjct: 103 KKLAQMGIEKQLDPPKNGLLVPNLVPVADQVIDNWKLLIKGLAQLLHVVPVFACSECGAV 162

Query: 94  YVGEKGHLIRSCGGYKRGPKNQVHEWIRGGLNDVLVSVEAFHRHHMFEKVNEHDERLNFE 153
           +V   GH IR C G     +   H W++G +NDVL+ VE++H +  F +  +H+ R  +E
Sbjct: 163 HVANVGHNIRDCNGPTNSQRRGSHSWVKGTINDVLIPVESYHMYDPFGRRIKHETRFEYE 222

Query: 154 RVPAVVELCWQAGANTNDENLSSSTW--NSVGGGGGGGGGGSGRDE-------------- 213
           R+PA+VELC QAG    +      T     +G      GG     E              
Sbjct: 223 RIPALVELCIQAGVEIPEYPCRRRTQPIRMMGKRVIDRGGYHKEPEKPQTSSSLSSPLAE 282

Query: 214 -----------PLSGNEMRLLATETLRAWETVRTGVQKLLMVYPTKICKYCSDVHVGPSV 273
                      P +  ++  +A ET+ A+E VR GV KL+  +  K C YCS+VHVGP  
Sbjct: 283 LDTLGVFERYPPPTPEDIPKIAQETMDAYEKVRLGVTKLMRKFTVKACGYCSEVHVGPWG 342

Query: 274 HQARPCGVFKCESWR-GSHFWEKADVDDLVPLKIVWHRRPQDPPVLVDEGRDYYGQSPAV 331
           H  + CG FK   WR G H W+ A VD++ P   VWH R      L    R +YG++PA+
Sbjct: 343 HSVKLCGEFK-HQWRDGKHGWQDALVDEVFPPNYVWHVRDLKGNPLTGNLRRFYGKAPAL 402

[Close]

BLAST of Carg03143 vs. TAIR 10
Match: AT1G64810.2 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 186.4 bits (472), Expect = 3.7e-47
Identity = 113/326 (34.66%), Postives = 163/326 (50.00%), Query Frame = 0


Query: 34  KKLRPM-ILKRIQNRANNCPIKGLIPVAQQVFEARAMLIHGVSTLLKAFPVVSCKFCPEV 93
           KKL  M I K++    N   +  L+PVA QV +   +LI G++ LL   PV +C  C  V
Sbjct: 127 KKLAQMGIEKQLDPPKNGLLVPNLVPVADQVIDNWKLLIKGLAQLLHVVPVFACSECGAV 186

Query: 94  YVGEKGHLIRSCGGYKRGPKNQVHEWIRGGLNDVLVSVEAFHRHHMFEKVNEHDERLNFE 153
           +V   GH IR C G     +   H W++G +NDVL+ VE++H +  F +  +H+ R  +E
Sbjct: 187 HVANVGHNIRDCNGPTNSQRRGSHSWVKGTINDVLIPVESYHMYDPFGRRIKHETRFEYE 246

Query: 154 RVPAVVELCWQAGANTNDENLSSSTW--NSVGGGGGGGGGGSGRDE-------------- 213
           R+PA+VELC QAG    +      T     +G      GG     E              
Sbjct: 247 RIPALVELCIQAGVEIPEYPCRRRTQPIRMMGKRVIDRGGYHKEPEKPQTSSSLSSPLAE 306

Query: 214 -----------PLSGNEMRLLATETLRAWETVRTGVQKLLMVYPTKICKYCSDVHVGPSV 273
                      P +  ++  +A ET+ A+E VR GV KL+  +  K C YCS+VHVGP  
Sbjct: 307 LDTLGVFERYPPPTPEDIPKIAQETMDAYEKVRLGVTKLMRKFTVKACGYCSEVHVGPWG 366

Query: 274 HQARPCGVFKCESWR-GSHFWEKADVDDLVPLKIVWHRRPQDPPVLVDEGRDYYGQSPAV 331
           H  + CG FK   WR G H W+ A VD++ P   VWH R      L    R +YG++PA+
Sbjct: 367 HSVKLCGEFK-HQWRDGKHGWQDALVDEVFPPNYVWHVRDLKGNPLTGNLRRFYGKAPAL 426

The following BLAST results are available for this feature:

BLAST of Carg03143 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7010722.1	3.0e-200	100.00	APO protein 4, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6570875.1	4.1e-197	99.70	APO protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022943578.1	2.0e-191	96.14	APO protein 4, mitochondrial-like [Cucurbita moschata]	[more]
XP_022986333.1	4.5e-188	94.96	APO protein 4, mitochondrial-like [Cucurbita maxima]	[more]
XP_038901944.1	7.5e-159	81.01	APO protein 4, mitochondrial [Benincasa hispida] >XP_038901945.1 APO protein 4, ...	[more]

BLAST of Carg03143 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSZ0	1.5e-93	54.36	APO protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO4 PE=2 SV=2	[more]
Q8W4A5	7.0e-51	35.74	APO protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO2 PE=2 SV=1	[more]
Q9XIR4	5.2e-46	34.66	APO protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO1 PE=2 SV=1	[more]
Q9FH50	2.8e-39	31.61	APO protein 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO3 PE=2 SV=1	[more]

BLAST of Carg03143 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FX59	9.5e-192	96.14	APO protein 4, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111448307 ...	[more]
A0A6J1JG78	2.2e-188	94.96	APO protein 4, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111484108 PE...	[more]
A0A6J1I7G7	3.3e-152	80.31	APO protein 4, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111469835 PE...	[more]
A0A0A0LQG5	3.3e-152	79.70	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G421010 PE=4 SV=1	[more]
A0A5A7TJ18	5.6e-152	79.39	APO protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1639G00180...	[more]

BLAST of Carg03143 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G21740.1	1.1e-94	54.36	Arabidopsis thaliana protein of unknown function (DUF794)	[more]
AT5G57930.1	5.0e-52	35.74	Arabidopsis thaliana protein of unknown function (DUF794)	[more]
AT5G57930.2	5.0e-52	35.74	Arabidopsis thaliana protein of unknown function (DUF794)	[more]
AT1G64810.1	3.7e-47	34.66	Arabidopsis thaliana protein of unknown function (DUF794)	[more]
AT1G64810.2	3.7e-47	34.66	Arabidopsis thaliana protein of unknown function (DUF794)	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023342	APO domain	PFAM	PF05634	APO_RNA-bind	coord: 29..168 e-value: 1.9E-38 score: 131.9 coord: 206..313 e-value: 1.4E-16 score: 60.5
IPR023342	APO domain	PROSITE	PS51499	APO	coord: 232..317 score: 18.887539
IPR023342	APO domain	PROSITE	PS51499	APO	coord: 85..170 score: 19.015347
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 172..199
None	No IPR available	PANTHER	PTHR10388	EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1	coord: 13..331
None	No IPR available	PANTHER	PTHR10388:SF20	APO PROTEIN 4, MITOCHONDRIAL	coord: 13..331

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg03143-RA	Carg03143-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003723	RNA binding