Carg03138 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg03138
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	septum-promoting GTP-binding protein 1-like
Location	Carg_Chr20: 2908006 .. 2909974 (-)
RNA-Seq Expression	Carg03138
Synteny	Carg03138

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGGCCATCCATAACGTTTTCCCAACGATGCCTCAACCTTGCCGGAAACAGCCTCTCCGCCTAAGCGTTAAGCGGCAGACCGCTCTCAAGCGCCACGCCGGCCAATTTTTGGACTCGATTCTTGCGTGTTACAGCTCCACCAAGCCTAAGGCGTATGCTGCGGTGCCCGATGATGCCATTGCGGCTTCTACGGTTGTCGAGGATGCTGGGTTTGCGGCGGAGACTCTAACACCATCCGTGTGCCATGATCATGGCCTGGACAAAGATTTGGTGTCTTTGAAAATTAGCCTGTTGGGTGATAGCCAAATTGGAAAGACAAGTTTTCTGGTAAGCTTGATTTTGTTTTGTTGGCGTTTTGGTTGGTCTCTGAGTTTTGTTTTTTTATTGTCTGTTTTGAAGGTTAAATATGTTGGGAATGAATTGGATGAAGGAAGGAGTGAGCAGACAGGATTGAATTTGATGGACAAAACACTTATGGTTAGAGGAGCAAGAATTTCTTATAGCATTTGTGGAAGTCGGACGCCTTAATTACATGAAGGACATGATTTTGATAACATGTTTTCGTGATTTTGCTTTGGACTTTCCTCCAAAAGGCCTCATAGCAAGGATGTATTCTTACTTATAAACTCGTGATCAATTAGCTAATGCAGGACTCCCTCCCAACAATTTTCGACAAATTTTCTCATTGGGTTTTCCCTTTCGGGATTCCTCTCACGGCTTTAAAACGCGTCTGCTAGGGGAAGGTTTTCATACCACCCTTGTAAAGGGTGTTTTGTTCTCCTCCCCAACCTACGTGGGACATCACAATCCACCTCCTTAGGAGCCCAGTGTCCTCGTTGGCACTCTTCCCTTTCTCTAATCGATGTGGGACCCCTGCCAAATCCACCCCCTTTGGAGCCAGTATCCTTACTGGCACACCGTCTCGTGTCTACCCTCTTTGGAGAACAGCCTTCTCGTTGGCACATAGTCCAGTGTCTGACTCTGATACAATCTGTAACAAACCAGACCCACTACTAGCCGATATTGTCCTCTTTGGACATAAGCTCTCGGATCAAGGCCGTTACACAAACAATTACCATTTCGACTAAATTAACCTAAACCCATCAGCTTAAATGATATTAGAACGTAGTTTTGTGTTTGAATTTGTATAAGAACGTTATGAGGATGTGGATGCAGGTAATAAGAAGTCTCAAGATTACATTCCAACGGCGTGTAAGGACTCTGTGGCAATTCTTTTTATGTTTGATCTAACAAGTAGGCGTACTCTAACCGGGTGAGGCTTGTTTCTGTTTGATCTAAATGACTTTGGCCTTCATGAATGGGAAGAATTAAACATAAGGGTTTTCCCTTTGGTTTGCAGCATTGTAAAGTGGTATAGACAAGCTCGAAAGTATAATCAGGTATAGTTTAGGTCTCGTTTGATAACGTTTTTAGAACGAAGTTTTGAATTTCTTTTTGTCCGTTCAAAGCTCTTTTCTTTGCAGAGAGCGATTCCAGTGTTGGTAGGAACAAAGTTCGATGATTTCATCCAACTCCCCATTGATTTGCAATGGGCAATTGCAAACGAGGTAAGAATTGAAATCATGAGTGTTTCTCTCTTTGATTTAAGCGTAACGACTCAAGCCCACCGCTAGTAGATACTGTCCTCTTTGGGTCTAGTAGATACTGTCCTCTTTGAGTTTTCCCTTTCAGGCTTCCCTCAAAGTTTTTAAAACTCGTTCTGTTAAGGAGAGGCAAGAGCCTACACCAACATCAATGGATGTAGGATCTCACAGAATGTCTCGTGATTTCCTTATTTTGACAGGCACGAGCTTACGCCAAGGCACTGAATGCTACATTGTTCTTCTCCAGTTCAACTTACAACATCAATGTCAATAAGATCTTTAAGTTCATCACAGCAAAGCTCTTCGACCTGCCGTGGACGGTTGAGCGCAACTTAACAGTCGGGGAGCCTATAATTGATTTCTAG

mRNA sequence

CAGGCCATCCATAACGTTTTCCCAACGATGCCTCAACCTTGCCGGAAACAGCCTCTCCGCCTAAGCGTTAAGCGGCAGACCGCTCTCAAGCGCCACGCCGGCCAATTTTTGGACTCGATTCTTGCGTGTTACAGCTCCACCAAGCCTAAGGCGTATGCTGCGGTGCCCGATGATGCCATTGCGGCTTCTACGGTTGTCGAGGATGCTGGGTTTGCGGCGGAGACTCTAACACCATCCGTGTGCCATGATCATGGCCTGGACAAAGATTTGGTGTCTTTGAAAATTAGCCTGTTGGGTGATAGCCAAATTGGAAAGACAAGTTTTCTGGTTAAATATGTTGGGAATGAATTGGATGAAGGAAGGAGTAATAAGAAGTCTCAAGATTACATTCCAACGGCGTGTAAGGACTCTGTGGCAATTCTTTTTATGTTTGATCTAACAAGTAGGCGTACTCTAACCGGCATTGTAAAGTGGTATAGACAAGCTCGAAAGTATAATCAGTTCATCACAGCAAAGCTCTTCGACCTGCCGTGGACGGTTGAGCGCAACTTAACAGTCGGGGAGCCTATAATTGATTTCTAG

Coding sequence (CDS)

ATGCCTCAACCTTGCCGGAAACAGCCTCTCCGCCTAAGCGTTAAGCGGCAGACCGCTCTCAAGCGCCACGCCGGCCAATTTTTGGACTCGATTCTTGCGTGTTACAGCTCCACCAAGCCTAAGGCGTATGCTGCGGTGCCCGATGATGCCATTGCGGCTTCTACGGTTGTCGAGGATGCTGGGTTTGCGGCGGAGACTCTAACACCATCCGTGTGCCATGATCATGGCCTGGACAAAGATTTGGTGTCTTTGAAAATTAGCCTGTTGGGTGATAGCCAAATTGGAAAGACAAGTTTTCTGGTTAAATATGTTGGGAATGAATTGGATGAAGGAAGGAGTAATAAGAAGTCTCAAGATTACATTCCAACGGCGTGTAAGGACTCTGTGGCAATTCTTTTTATGTTTGATCTAACAAGTAGGCGTACTCTAACCGGCATTGTAAAGTGGTATAGACAAGCTCGAAAGTATAATCAGTTCATCACAGCAAAGCTCTTCGACCTGCCGTGGACGGTTGAGCGCAACTTAACAGTCGGGGAGCCTATAATTGATTTCTAG

Protein sequence

MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYAAVPDDAIAASTVVEDAGFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSNKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQARKYNQFITAKLFDLPWTVERNLTVGEPIIDF

Homology

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BLAST of Carg03138 vs. NCBI nr
Match: KAG7010727.1 (spg1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 369.0 bits (946), Expect = 2.4e-98
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYAAVPDDAIAASTVVEDA 60
           MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYAAVPDDAIAASTVVEDA
Sbjct: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYAAVPDDAIAASTVVEDA 60

Query: 61  GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSNKKSQDY 120
           GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSNKKSQDY
Sbjct: 61  GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSNKKSQDY 120

Query: 121 IPTACKDSVAILFMFDLTSRRTLTGIVKWYRQARKYNQFITAKLFDLPWTVERNLTVGEP 180
           IPTACKDSVAILFMFDLTSRRTLTGIVKWYRQARKYNQFITAKLFDLPWTVERNLTVGEP
Sbjct: 121 IPTACKDSVAILFMFDLTSRRTLTGIVKWYRQARKYNQFITAKLFDLPWTVERNLTVGEP 180

Query: 181 IIDF 185
           IIDF
Sbjct: 181 IIDF 184

[Close]

BLAST of Carg03138 vs. NCBI nr
Match: KAG6570880.1 (Glutamate receptor 3.4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 326.2 bits (835), Expect = 1.8e-85
Identity = 183/268 (68.28%), Postives = 183/268 (68.28%), Query Frame = 0


Query: 1    MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYAAVPDDAIAASTVVEDA 60
            MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYAAVPDDA AASTVVEDA
Sbjct: 898  MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYAAVPDDATAASTVVEDA 957

Query: 61   GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRS------- 120
            GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRS       
Sbjct: 958  GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL 1017

Query: 121  ---------------------NKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180
                                 NKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ
Sbjct: 1018 MDKTLMVRGARISYSIWEVEGNKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 1077

Query: 181  ARKYNQ------------------------------------------------------ 185
            ARKYNQ                                                      
Sbjct: 1078 ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARAYAKALNATLFFSSSTYNINVNKI 1137

[Close]

BLAST of Carg03138 vs. NCBI nr
Match: XP_023513332.1 (septum-promoting GTP-binding protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 322.4 bits (825), Expect = 2.5e-84
Identity = 181/268 (67.54%), Postives = 182/268 (67.91%), Query Frame = 0


Query: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYAAVPDDAIAASTVVEDA 60
           MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAY AVPDDA AASTVVEDA
Sbjct: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYPAVPDDATAASTVVEDA 60

Query: 61  GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRS------- 120
           GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRS       
Sbjct: 61  GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL 120

Query: 121 ---------------------NKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180
                                +KKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ
Sbjct: 121 MDKTLLVRGARISYSIWEVGGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180

Query: 181 ARKYNQ------------------------------------------------------ 185
           ARKYNQ                                                      
Sbjct: 181 ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARAYAKALNATLFFSSSTYNINVNKI 240

[Close]

BLAST of Carg03138 vs. NCBI nr
Match: XP_022944635.1 (septum-promoting GTP-binding protein 1-like [Cucurbita moschata])

HSP 1 Score: 318.2 bits (814), Expect = 4.8e-83
Identity = 178/268 (66.42%), Postives = 182/268 (67.91%), Query Frame = 0


Query: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYAAVPDDAIAASTVVEDA 60
           MPQPCRKQPLRLSVKRQTALKRHAGQFLDSIL+CYSSTKPKAY AVPD+A AASTVVEDA
Sbjct: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILSCYSSTKPKAYPAVPDEATAASTVVEDA 60

Query: 61  GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRS------- 120
           GFAAE+LTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRS       
Sbjct: 61  GFAAESLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL 120

Query: 121 ---------------------NKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180
                                +KKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ
Sbjct: 121 MDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180

Query: 181 ARKYNQ------------------------------------------------------ 185
           ARKYNQ                                                      
Sbjct: 181 ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARAYAKALNATLFFSSSTYNINVNKI 240

[Close]

BLAST of Carg03138 vs. NCBI nr
Match: XP_022986354.1 (septum-promoting GTP-binding protein 1-like [Cucurbita maxima])

HSP 1 Score: 317.0 bits (811), Expect = 1.1e-82
Identity = 178/268 (66.42%), Postives = 181/268 (67.54%), Query Frame = 0


Query: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYAAVPDDAIAASTVVEDA 60
           MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAY AVP +A AASTVV+DA
Sbjct: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYPAVPTEATAASTVVDDA 60

Query: 61  GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRS------- 120
           GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRS       
Sbjct: 61  GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL 120

Query: 121 ---------------------NKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180
                                +KKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ
Sbjct: 121 MDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180

Query: 181 ARKYNQ------------------------------------------------------ 185
           ARKYNQ                                                      
Sbjct: 181 ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARTYAKALNATLFFSSSTYNINVNKI 240

[Close]

BLAST of Carg03138 vs. ExPASy Swiss-Prot
Match: P87027 (Septum-promoting GTP-binding protein 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=spg1 PE=1 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.3e-11
Identity = 38/108 (35.19%), Postives = 56/108 (51.85%), Query Frame = 0


Query: 79  KDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRS------------------------- 138
           K+ V++K+ ++GDS IGKTS +V YV    DE  +                         
Sbjct: 6   KNNVTIKVGMIGDSSIGKTSLMVTYVQGSFDEESTQTLGVNFMEKTISIRNTEITFSIWD 65

Query: 139 ---NKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQARKYNQ 159
               ++  + +P  C D+VAILFMFDL+ + TL  I +WYRQAR +N+
Sbjct: 66  LGGQREFVNMLPMVCNDAVAILFMFDLSRKSTLNSIKEWYRQARGFNK 113

[Close]

BLAST of Carg03138 vs. ExPASy Swiss-Prot
Match: P38987 (Protein TEM1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TEM1 PE=1 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 3.4e-07
Identity = 39/120 (32.50%), Postives = 59/120 (49.17%), Query Frame = 0


Query: 68  TPSVCHDHGLD--KDLVSLKISLLGDSQIGKTSFLVKYVGNELD------------EGRS 127
           TPS   ++ +   ++ V +++ L+GD+Q+GKTS +VKYV N  D            + + 
Sbjct: 3   TPSTGANNSIPAVRNQVEVQVGLVGDAQVGKTSLMVKYVQNIYDKEYTQTLGVNFLKRKV 62

Query: 128 NKKSQDYI----------------PTACKDSVAILFMFDLTSRRTLTGIVKWYRQARKYN 158
           + +S D I                P A   S  I+F+FDLT   TL+ I +WYRQA   N
Sbjct: 63  SIRSTDIIFSIMDLGGQREFINMLPIATVGSSVIIFLFDLTRPETLSSIKEWYRQAYGLN 122

[Close]

BLAST of Carg03138 vs. ExPASy Swiss-Prot
Match: Q9VP48 (Ras-related protein Rab-26 OS=Drosophila melanogaster OX=7227 GN=Rab26 PE=2 SV=2)

HSP 1 Score: 46.2 bits (108), Expect = 4.6e-04
Identity = 34/119 (28.57%), Postives = 49/119 (41.18%), Query Frame = 0


Query: 69  PSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEG----------------- 128
           PS  H    D D V+ K  LLGDS +GKTSFLVKY   E   G                 
Sbjct: 177 PSNAHQQPFD-DTVNHKTILLGDSGVGKTSFLVKYNTGEFRLGSFSATVGIALTNKVVVV 236

Query: 129 ------------RSNKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQARKYNQ 159
                          ++ +       +D+ A+L ++D+T++ T   I  W  + R+Y Q
Sbjct: 237 DGTRVKLQIWDTAGQERFRSVTHAYYRDAHALLLLYDVTNKTTYDNIRAWLGEIREYAQ 294

[Close]

BLAST of Carg03138 vs. ExPASy TrEMBL
Match: A0A6J1FYS5 (septum-promoting GTP-binding protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449038 PE=4 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 2.3e-83
Identity = 178/268 (66.42%), Postives = 182/268 (67.91%), Query Frame = 0


Query: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYAAVPDDAIAASTVVEDA 60
           MPQPCRKQPLRLSVKRQTALKRHAGQFLDSIL+CYSSTKPKAY AVPD+A AASTVVEDA
Sbjct: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILSCYSSTKPKAYPAVPDEATAASTVVEDA 60

Query: 61  GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRS------- 120
           GFAAE+LTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRS       
Sbjct: 61  GFAAESLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL 120

Query: 121 ---------------------NKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180
                                +KKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ
Sbjct: 121 MDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180

Query: 181 ARKYNQ------------------------------------------------------ 185
           ARKYNQ                                                      
Sbjct: 181 ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARAYAKALNATLFFSSSTYNINVNKI 240

[Close]

BLAST of Carg03138 vs. ExPASy TrEMBL
Match: A0A6J1JFU6 (septum-promoting GTP-binding protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111484119 PE=4 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 5.2e-83
Identity = 178/268 (66.42%), Postives = 181/268 (67.54%), Query Frame = 0


Query: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYAAVPDDAIAASTVVEDA 60
           MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAY AVP +A AASTVV+DA
Sbjct: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYPAVPTEATAASTVVDDA 60

Query: 61  GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRS------- 120
           GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRS       
Sbjct: 61  GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL 120

Query: 121 ---------------------NKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180
                                +KKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ
Sbjct: 121 MDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180

Query: 181 ARKYNQ------------------------------------------------------ 185
           ARKYNQ                                                      
Sbjct: 181 ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARTYAKALNATLFFSSSTYNINVNKI 240

[Close]

BLAST of Carg03138 vs. ExPASy TrEMBL
Match: A0A6J1I4P4 (septum-promoting GTP-binding protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111469846 PE=4 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 5.0e-54
Identity = 137/276 (49.64%), Postives = 153/276 (55.43%), Query Frame = 0


Query: 1   MPQPCRKQPLRLSVKRQ-----TALKRHAGQFLDSILACYSSTKPKAYAAVPDDAIAAS- 60
           MPQPCRKQPL   +KRQ     TALKR   QFL+SIL CYS  KP+    V DDA  AS 
Sbjct: 19  MPQPCRKQPLNFGLKRQMHHPATALKRRTRQFLESILTCYSK-KPQTCRTVRDDAATASI 78

Query: 61  --TVVEDAGFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGR 120
               V D GF AET TP +CH+H  DKDLV LKISLLGDSQIGKT FL KYVGNE++EG+
Sbjct: 79  SPRAVADGGFEAETRTP-LCHNHSQDKDLVPLKISLLGDSQIGKTCFLEKYVGNEMEEGK 138

Query: 121 S----------------------------NKKSQDYIPTACKDSVAILFMFDLTSRRTLT 180
           S                            +KKSQD+IPTACKDSVAILFMFDLTSRRTL 
Sbjct: 139 SEQRGLNLMDKTLMVRGARISYSIWEVGGDKKSQDHIPTACKDSVAILFMFDLTSRRTLK 198

Query: 181 GIVKWYRQARKYNQ---------------------------------------------- 185
            +++WYRQA K+NQ                                              
Sbjct: 199 SVLRWYRQAWKWNQAAIPVLIGTKFDDFIQLPIDLQWAIACEARTYAKALNATLFFSSAT 258

[Close]

BLAST of Carg03138 vs. ExPASy TrEMBL
Match: A0A6J1G5T0 (septum-promoting GTP-binding protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111451069 PE=4 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 1.5e-53
Identity = 137/276 (49.64%), Postives = 153/276 (55.43%), Query Frame = 0


Query: 1   MPQPCRKQPLRLSVKRQ-----TALKRHAGQFLDSILACYSSTKPKAYAAVPDDAIAAS- 60
           MPQPCRKQPL   +KRQ     TALKR   QFL+SIL CYS  KP+    V DDA  AS 
Sbjct: 19  MPQPCRKQPLNFGLKRQMHHPATALKRRTRQFLESILTCYSK-KPQTCRTVCDDAATASI 78

Query: 61  --TVVEDAGFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGR 120
               V D G  AET TP +CH+H  DKDLV LKISLLGDSQIGKTSFL KYVGNE++EG+
Sbjct: 79  SPRAVADGGSEAETRTP-LCHNHSQDKDLVPLKISLLGDSQIGKTSFLEKYVGNEMEEGK 138

Query: 121 S----------------------------NKKSQDYIPTACKDSVAILFMFDLTSRRTLT 180
           S                            +KKSQD+IPTACKDSVAILFMFDLTSRRTL 
Sbjct: 139 SEQRGLNLMDKTLMVRGARISYSIWEVGGDKKSQDHIPTACKDSVAILFMFDLTSRRTLK 198

Query: 181 GIVKWYRQARKYNQ---------------------------------------------- 185
            +++WYRQA K+NQ                                              
Sbjct: 199 SVLRWYRQAWKWNQAAIPVLIGTKFDDFIQLPIDLQWAIASEARAYAKALNATLFFSSAT 258

[Close]

BLAST of Carg03138 vs. ExPASy TrEMBL
Match: A0A5A7TPV3 (Septum-promoting GTP-binding protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold27G00460 PE=4 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.6e-52
Identity = 134/271 (49.45%), Postives = 153/271 (56.46%), Query Frame = 0


Query: 1   MPQPCRKQPL-RLSVKRQTALKRHAGQFLDSILACYSSTKPKAYAAVPDDAIAAST--VV 60
           MP+P RK PL R +++R+TALKR A  F DSIL CYSS KP+AY    DD   AST  VV
Sbjct: 1   MPRPSRKHPLRRFTLRRRTALKRRAWHFFDSILTCYSSKKPQAY----DDVALASTSVVV 60

Query: 61  EDAGFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRS---- 120
            D  F AETL PS+CH    ++DLVSLKISLLGDSQIGKTSFLVKYVGNE+DEG+S    
Sbjct: 61  VDEEFVAETLAPSLCH----NQDLVSLKISLLGDSQIGKTSFLVKYVGNEMDEGKSEQTG 120

Query: 121 ------------------------NKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKW 180
                                   + KSQDYI TACK SVAIL+MFDLTSRRTL   V+W
Sbjct: 121 LKLMDKTLMVRGARISYSIWEVEGDSKSQDYISTACKGSVAILYMFDLTSRRTLNNTVRW 180

Query: 181 YRQARKYN---------------------------------------------------- 185
           YRQAR++N                                                    
Sbjct: 181 YRQARRWNKTAIPVLIGTKFDDFIQLPIDLQWAIASEARSYAKALNATLFFSSATYNINV 240

[Close]

BLAST of Carg03138 vs. TAIR 10
Match: AT3G21700.3 (Ras-related small GTP-binding family protein )

HSP 1 Score: 128.3 bits (321), Expect = 6.6e-30
Identity = 105/292 (35.96%), Postives = 127/292 (43.49%), Query Frame = 0


Query: 1   MPQPCRK----QPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAY----AAVPDDAI- 60
           M Q C K      LR  V R+  + R   + L S +   +  K + Y     A+P   + 
Sbjct: 1   MAQSCLKIVRINNLRNRVNRRILILRRFTRLLWSRIVACTPGKSRRYLLLSRALPSPTVS 60

Query: 61  --------AASTVVEDAGFAAETL-TPSVCHDHG-----LDKDLVSLKISLLGDSQIGKT 120
                   A   VV   G   E +   SV +D+       D DLVSLKISLLGD +IGKT
Sbjct: 61  RPSPSPIPAVDVVVGGGGGGGEFVRRSSVVYDNDNSHRRSDSDLVSLKISLLGDPEIGKT 120

Query: 121 SFLVKYVG-------NELDEG----------------------RSNKKSQDYIPTACKDS 180
           SFL KYVG        EL++G                         ++S+D IP ACKDS
Sbjct: 121 SFLAKYVGEEKEVEMRELEKGINCTDKTLYMGGARISYSIWELEGAERSRDQIPVACKDS 180

Query: 181 VAILFMFDLTSRRTLTGIVKWYRQARKYNQ------------------------------ 185
           VAILFMFDLTSR TL  ++ WY+QARK NQ                              
Sbjct: 181 VAILFMFDLTSRCTLNSVISWYQQARKSNQTAIPVMVGTKFDEFIQLPIDLQWTIASQAR 240

[Close]

BLAST of Carg03138 vs. TAIR 10
Match: AT3G21700.1 (Ras-related small GTP-binding family protein )

HSP 1 Score: 127.9 bits (320), Expect = 8.6e-30
Identity = 105/291 (36.08%), Postives = 127/291 (43.64%), Query Frame = 0


Query: 1   MPQPCRK----QPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAY----AAVPDDAI- 60
           M Q C K      LR  V R+  + R   + L S +   +  K + Y     A+P   + 
Sbjct: 1   MAQSCLKIVRINNLRNRVNRRILILRRFTRLLWSRIVACTPGKSRRYLLLSRALPSPTVS 60

Query: 61  --------AASTVVEDAGFAAETL-TPSVCHDHG-----LDKDLVSLKISLLGDSQIGKT 120
                   A   VV   G   E +   SV +D+       D DLVSLKISLLGD +IGKT
Sbjct: 61  RPSPSPIPAVDVVVGGGGGGGEFVRRSSVVYDNDNSHRRSDSDLVSLKISLLGDPEIGKT 120

Query: 121 SFLVKYVG-------NELDEG---------------------RSNKKSQDYIPTACKDSV 180
           SFL KYVG        EL++G                        ++S+D IP ACKDSV
Sbjct: 121 SFLAKYVGEEKEVEMRELEKGINCTDKTLYMGGARISYSIWELEAERSRDQIPVACKDSV 180

Query: 181 AILFMFDLTSRRTLTGIVKWYRQARKYNQ------------------------------- 185
           AILFMFDLTSR TL  ++ WY+QARK NQ                               
Sbjct: 181 AILFMFDLTSRCTLNSVISWYQQARKSNQTAIPVMVGTKFDEFIQLPIDLQWTIASQART 240

[Close]

BLAST of Carg03138 vs. TAIR 10
Match: AT5G54840.1 (Ras-related small GTP-binding family protein )

HSP 1 Score: 119.8 bits (299), Expect = 2.3e-27
Identity = 74/191 (38.74%), Postives = 90/191 (47.12%), Query Frame = 0


Query: 78  DKDLVSLKISLLGDSQIGKTSFLVKYVGNE----------------------------LD 137
           D DLVSLKISLLGD Q GKT+F+VKYVG+E                             D
Sbjct: 96  DSDLVSLKISLLGDCQTGKTTFVVKYVGDENQSFLEMTGLNLMDKTFYVQGVTISFSIWD 155

Query: 138 EGRSNKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQARKYN------------ 185
            G   K+S+D+IP ACKD+VAILFMFDLTSR TL  +  WY QARK+N            
Sbjct: 156 VGGDEKRSKDHIPIACKDAVAILFMFDLTSRSTLNSVFGWYSQARKWNKTAIPILIGTKF 215

[Close]

BLAST of Carg03138 vs. TAIR 10
Match: AT5G54840.2 (Ras-related small GTP-binding family protein )

HSP 1 Score: 103.6 bits (257), Expect = 1.7e-22
Identity = 57/116 (49.14%), Postives = 68/116 (58.62%), Query Frame = 0


Query: 78  DKDLVSLKISLLGDSQIGKTSFLVKYVGNE----------------------------LD 137
           D DLVSLKISLLGD Q GKT+F+VKYVG+E                             D
Sbjct: 96  DSDLVSLKISLLGDCQTGKTTFVVKYVGDENQSFLEMTGLNLMDKTFYVQGVTISFSIWD 155

Query: 138 EGRSNKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQARKYNQFITAKLF 166
            G   K+S+D+IP ACKD+VAILFMFDLTSR TL  +  WY QARK+N+     +F
Sbjct: 156 VGGDEKRSKDHIPIACKDAVAILFMFDLTSRSTLNSVFGWYSQARKWNKVRLISMF 211

[Close]

BLAST of Carg03138 vs. TAIR 10
Match: AT3G21700.2 (Ras-related small GTP-binding family protein )

HSP 1 Score: 100.9 bits (250), Expect = 1.1e-21
Identity = 87/235 (37.02%), Postives = 109/235 (46.38%), Query Frame = 0


Query: 1   MPQPCRK----QPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAY----AAVPDDAI- 60
           M Q C K      LR  V R+  + R   + L S +   +  K + Y     A+P   + 
Sbjct: 1   MAQSCLKIVRINNLRNRVNRRILILRRFTRLLWSRIVACTPGKSRRYLLLSRALPSPTVS 60

Query: 61  --------AASTVVEDAGFAAETL-TPSVCHDHG-----LDKDLVSLKISLLGDSQIGKT 120
                   A   VV   G   E +   SV +D+       D DLVSLKISLLGD +IGKT
Sbjct: 61  RPSPSPIPAVDVVVGGGGGGGEFVRRSSVVYDNDNSHRRSDSDLVSLKISLLGDPEIGKT 120

Query: 121 SFLVKYVG-------NELDEG---------------------RSNKKSQDYIPTACKDSV 172
           SFL KYVG        EL++G                        ++S+D IP ACKDSV
Sbjct: 121 SFLAKYVGEEKEVEMRELEKGINCTDKTLYMGGARISYSIWELEAERSRDQIPVACKDSV 180

The following BLAST results are available for this feature:

BLAST of Carg03138 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7010727.1	2.4e-98	100.00	spg1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6570880.1	1.8e-85	68.28	Glutamate receptor 3.4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023513332.1	2.5e-84	67.54	septum-promoting GTP-binding protein 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022944635.1	4.8e-83	66.42	septum-promoting GTP-binding protein 1-like [Cucurbita moschata]	[more]
XP_022986354.1	1.1e-82	66.42	septum-promoting GTP-binding protein 1-like [Cucurbita maxima]	[more]

BLAST of Carg03138 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P87027	1.3e-11	35.19	Septum-promoting GTP-binding protein 1 OS=Schizosaccharomyces pombe (strain 972 ...	[more]
P38987	3.4e-07	32.50	Protein TEM1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 ...	[more]
Q9VP48	4.6e-04	28.57	Ras-related protein Rab-26 OS=Drosophila melanogaster OX=7227 GN=Rab26 PE=2 SV=2	[more]

BLAST of Carg03138 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FYS5	2.3e-83	66.42	septum-promoting GTP-binding protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1JFU6	5.2e-83	66.42	septum-promoting GTP-binding protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1I4P4	5.0e-54	49.64	septum-promoting GTP-binding protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1G5T0	1.5e-53	49.64	septum-promoting GTP-binding protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A5A7TPV3	1.6e-52	49.45	Septum-promoting GTP-binding protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of Carg03138 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G21700.3	6.6e-30	35.96	Ras-related small GTP-binding family protein	[more]
AT3G21700.1	8.6e-30	36.08	Ras-related small GTP-binding family protein	[more]
AT5G54840.1	2.3e-27	38.74	Ras-related small GTP-binding family protein	[more]
AT5G54840.2	1.7e-22	49.14	Ras-related small GTP-binding family protein	[more]
AT3G21700.2	1.1e-21	37.02	Ras-related small GTP-binding family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 77..168 e-value: 3.1E-7 score: 31.8
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 84..158
None	No IPR available	PANTHER	PTHR47978:SF29	MONOMERIC G-PROTEIN	coord: 113..158 coord: 158..183
None	No IPR available	PANTHER	PTHR47978	FAMILY NOT NAMED	coord: 113..158 coord: 158..183
None	No IPR available	PANTHER	PTHR47978	FAMILY NOT NAMED	coord: 38..116
None	No IPR available	PANTHER	PTHR47978:SF29	MONOMERIC G-PROTEIN	coord: 38..116

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg03138-RA	Carg03138-RA	mRNA