Carg03133 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg03133
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	ThiF domain-containing protein
Location	Carg_Chr20: 2938901 .. 2939290 (+)
RNA-Seq Expression	Carg03133
Synteny	Carg03133

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATCGCAGAGTTCAATTCTCGCACAAACAACGTGCAAATCGCACGGAGCAATTCCACAAGTACTTGAAGCCCGGTGCCCTGGCGAAGATCCGGGACTCGCGAATCAGCGCCAGATTGTACCGATACAACTCGCTATCTCAGATCTATCTCCGCCGTACTGCTTCTCCGGAGTCGTCTCCACTTTCCTCTATCGGTCAATCGGAGATTGACGTGGATGATTTCCCTTCTTTGTCTGGAAGGGTTTACGGTCCCTTATGCCTCAAGAGGAAGAAGTTCTCGGGAATTAAGTCTACGTGGTTCCCGAGCCCTAATTTGCAGACTCCAACTGCCGATTTAAGTGGAGACTCGATTATTGATGCGTTTAATAGCGATGTTGCTGCGCATTGA

mRNA sequence

ATGAATCGCAGAGTTCAATTCTCGCACAAACAACGTGCAAATCGCACGGAGCAATTCCACAAGTACTTGAAGCCCGGTGCCCTGGCGAAGATCCGGGACTCGCGAATCAGCGCCAGATTGTACCGATACAACTCGCTATCTCAGATCTATCTCCGCCGTACTGCTTCTCCGGAGTCGTCTCCACTTTCCTCTATCGGTCAATCGGAGATTGACGTGGATGATTTCCCTTCTTTGTCTGGAAGGGTTTACGGTCCCTTATGCCTCAAGAGGAAGAAGTTCTCGGGAATTAAGTCTACGTGGTTCCCGAGCCCTAATTTGCAGACTCCAACTGCCGATTTAAGTGGAGACTCGATTATTGATGCGTTTAATAGCGATGTTGCTGCGCATTGA

Coding sequence (CDS)

ATGAATCGCAGAGTTCAATTCTCGCACAAACAACGTGCAAATCGCACGGAGCAATTCCACAAGTACTTGAAGCCCGGTGCCCTGGCGAAGATCCGGGACTCGCGAATCAGCGCCAGATTGTACCGATACAACTCGCTATCTCAGATCTATCTCCGCCGTACTGCTTCTCCGGAGTCGTCTCCACTTTCCTCTATCGGTCAATCGGAGATTGACGTGGATGATTTCCCTTCTTTGTCTGGAAGGGTTTACGGTCCCTTATGCCTCAAGAGGAAGAAGTTCTCGGGAATTAAGTCTACGTGGTTCCCGAGCCCTAATTTGCAGACTCCAACTGCCGATTTAAGTGGAGACTCGATTATTGATGCGTTTAATAGCGATGTTGCTGCGCATTGA

Protein sequence

MNRRVQFSHKQRANRTEQFHKYLKPGALAKIRDSRISARLYRYNSLSQIYLRRTASPESSPLSSIGQSEIDVDDFPSLSGRVYGPLCLKRKKFSGIKSTWFPSPNLQTPTADLSGDSIIDAFNSDVAAH

Homology

[Close]

BLAST of Carg03133 vs. NCBI nr
Match: XP_022944248.1 (uncharacterized protein LOC111448755 [Cucurbita moschata] >KAG6570884.1 hypothetical protein SDJN03_29799, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010732.1 hypothetical protein SDJN02_27528, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 257.7 bits (657), Expect = 5.4e-65
Identity = 129/129 (100.00%), Postives = 129/129 (100.00%), Query Frame = 0


Query: 1   MNRRVQFSHKQRANRTEQFHKYLKPGALAKIRDSRISARLYRYNSLSQIYLRRTASPESS 60
           MNRRVQFSHKQRANRTEQFHKYLKPGALAKIRDSRISARLYRYNSLSQIYLRRTASPESS
Sbjct: 1   MNRRVQFSHKQRANRTEQFHKYLKPGALAKIRDSRISARLYRYNSLSQIYLRRTASPESS 60

Query: 61  PLSSIGQSEIDVDDFPSLSGRVYGPLCLKRKKFSGIKSTWFPSPNLQTPTADLSGDSIID 120
           PLSSIGQSEIDVDDFPSLSGRVYGPLCLKRKKFSGIKSTWFPSPNLQTPTADLSGDSIID
Sbjct: 61  PLSSIGQSEIDVDDFPSLSGRVYGPLCLKRKKFSGIKSTWFPSPNLQTPTADLSGDSIID 120

Query: 121 AFNSDVAAH 130
           AFNSDVAAH
Sbjct: 121 AFNSDVAAH 129

[Close]

BLAST of Carg03133 vs. NCBI nr
Match: XP_023513131.1 (uncharacterized protein LOC111777660 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 240.7 bits (613), Expect = 6.8e-60
Identity = 119/129 (92.25%), Postives = 125/129 (96.90%), Query Frame = 0


Query: 1   MNRRVQFSHKQRANRTEQFHKYLKPGALAKIRDSRISARLYRYNSLSQIYLRRTASPESS 60
           MNRRVQFSHKQRANRTEQFHKYLKPGALAK+RDSRISAR YRYNSLSQIYLRRTAS ESS
Sbjct: 1   MNRRVQFSHKQRANRTEQFHKYLKPGALAKMRDSRISARSYRYNSLSQIYLRRTASLESS 60

Query: 61  PLSSIGQSEIDVDDFPSLSGRVYGPLCLKRKKFSGIKSTWFPSPNLQTPTADLSGDSIID 120
           PLS+IGQSEIDVDDFPSL+GRVYGPLCLKRKKF+GIKSTWFPSPNLQTPT DL+G+ IID
Sbjct: 61  PLSAIGQSEIDVDDFPSLAGRVYGPLCLKRKKFAGIKSTWFPSPNLQTPTTDLTGEPIID 120

Query: 121 AFNSDVAAH 130
           AFNSDVAAH
Sbjct: 121 AFNSDVAAH 129

[Close]

BLAST of Carg03133 vs. NCBI nr
Match: XP_022986202.1 (uncharacterized protein LOC111484017 [Cucurbita maxima])

HSP 1 Score: 233.8 bits (595), Expect = 8.3e-58
Identity = 115/129 (89.15%), Postives = 123/129 (95.35%), Query Frame = 0


Query: 1   MNRRVQFSHKQRANRTEQFHKYLKPGALAKIRDSRISARLYRYNSLSQIYLRRTASPESS 60
           MNRRVQ SHKQRANRTEQFHK+LKPGALAK+RDSRISAR YRYNSLSQI+LRRTAS ESS
Sbjct: 1   MNRRVQLSHKQRANRTEQFHKFLKPGALAKMRDSRISARSYRYNSLSQIHLRRTASLESS 60

Query: 61  PLSSIGQSEIDVDDFPSLSGRVYGPLCLKRKKFSGIKSTWFPSPNLQTPTADLSGDSIID 120
           PLS+IGQSEIDVDDFPSLSGRVYGPLCLKRKK +GIK+TWFPSPNLQTPT DL+GD I+D
Sbjct: 61  PLSAIGQSEIDVDDFPSLSGRVYGPLCLKRKKLAGIKTTWFPSPNLQTPTTDLTGDHIVD 120

Query: 121 AFNSDVAAH 130
           AFNSDVAAH
Sbjct: 121 AFNSDVAAH 129

[Close]

BLAST of Carg03133 vs. NCBI nr
Match: XP_038902395.1 (uncharacterized protein LOC120089036 [Benincasa hispida])

HSP 1 Score: 193.4 bits (490), Expect = 1.2e-45
Identity = 95/130 (73.08%), Postives = 113/130 (86.92%), Query Frame = 0


Query: 1   MNRRVQFSHKQRANRTEQFHKYLKPGALAKIRDSRISARLYRYNSLSQIYLRRTASPESS 60
           M R+++FS K  A+RTEQFHKYLKPGALA++RDS+ISA+ YR+NSLSQIYLRRT+   SS
Sbjct: 1   MKRKLKFSPKPYADRTEQFHKYLKPGALAQMRDSQISAKWYRFNSLSQIYLRRTSPLPSS 60

Query: 61  PLSSIGQSEID-VDDFPSLSGRVYGPLCLKRKKFSGIKSTWFPSPNLQTPTADLSGDSII 120
           PLS+IGQSEID VDDFPSL+GR YGP CLKRKK + IK+TWFPSPNLQTPT D++ D ++
Sbjct: 61  PLSTIGQSEIDVVDDFPSLAGRGYGPFCLKRKKLAAIKATWFPSPNLQTPTTDITRDPVV 120

Query: 121 DAFNSDVAAH 130
           D FNSDVAAH
Sbjct: 121 DVFNSDVAAH 130

[Close]

BLAST of Carg03133 vs. NCBI nr
Match: PON83836.1 (hypothetical protein TorRG33x02_203870 [Trema orientale])

HSP 1 Score: 117.9 bits (294), Expect = 6.7e-23
Identity = 68/130 (52.31%), Postives = 87/130 (66.92%), Query Frame = 0


Query: 1   MNRRVQFSHKQRANRTEQFHKYLKPGALAKIRDSRISARLYRYNSLSQIYLRRTASPESS 60
           MNRR +  H+   +RTE F KYLKPGALA+IRDSRISAR +R  SL+QI   R++ P S 
Sbjct: 1   MNRRTRAIHRSSPSRTEPFLKYLKPGALAQIRDSRISARSHRVVSLAQI---RSSQPSSP 60

Query: 61  PLSSIGQ-SEIDVDDFPSLSGRVYGPLCLKRKKFSGIKSTWFPSPNLQTPTADLSGDSII 120
           P S+ GQ S   +D FP  SGRV+GP C +RKK    K+  FPS N  +P  D + D I+
Sbjct: 61  PPSTPGQLSVAAMDGFPCFSGRVFGPRCPQRKKLVASKAVLFPSQNPSSPVLD-AADPIM 120

Query: 121 DAFNSDVAAH 130
           D F++D+AAH
Sbjct: 121 DVFSTDIAAH 126

[Close]

BLAST of Carg03133 vs. ExPASy TrEMBL
Match: A0A6J1FXV4 (uncharacterized protein LOC111448755 OS=Cucurbita moschata OX=3662 GN=LOC111448755 PE=4 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 2.6e-65
Identity = 129/129 (100.00%), Postives = 129/129 (100.00%), Query Frame = 0


Query: 1   MNRRVQFSHKQRANRTEQFHKYLKPGALAKIRDSRISARLYRYNSLSQIYLRRTASPESS 60
           MNRRVQFSHKQRANRTEQFHKYLKPGALAKIRDSRISARLYRYNSLSQIYLRRTASPESS
Sbjct: 1   MNRRVQFSHKQRANRTEQFHKYLKPGALAKIRDSRISARLYRYNSLSQIYLRRTASPESS 60

Query: 61  PLSSIGQSEIDVDDFPSLSGRVYGPLCLKRKKFSGIKSTWFPSPNLQTPTADLSGDSIID 120
           PLSSIGQSEIDVDDFPSLSGRVYGPLCLKRKKFSGIKSTWFPSPNLQTPTADLSGDSIID
Sbjct: 61  PLSSIGQSEIDVDDFPSLSGRVYGPLCLKRKKFSGIKSTWFPSPNLQTPTADLSGDSIID 120

Query: 121 AFNSDVAAH 130
           AFNSDVAAH
Sbjct: 121 AFNSDVAAH 129

[Close]

BLAST of Carg03133 vs. ExPASy TrEMBL
Match: A0A6J1J6X7 (uncharacterized protein LOC111484017 OS=Cucurbita maxima OX=3661 GN=LOC111484017 PE=4 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 4.0e-58
Identity = 115/129 (89.15%), Postives = 123/129 (95.35%), Query Frame = 0


Query: 1   MNRRVQFSHKQRANRTEQFHKYLKPGALAKIRDSRISARLYRYNSLSQIYLRRTASPESS 60
           MNRRVQ SHKQRANRTEQFHK+LKPGALAK+RDSRISAR YRYNSLSQI+LRRTAS ESS
Sbjct: 1   MNRRVQLSHKQRANRTEQFHKFLKPGALAKMRDSRISARSYRYNSLSQIHLRRTASLESS 60

Query: 61  PLSSIGQSEIDVDDFPSLSGRVYGPLCLKRKKFSGIKSTWFPSPNLQTPTADLSGDSIID 120
           PLS+IGQSEIDVDDFPSLSGRVYGPLCLKRKK +GIK+TWFPSPNLQTPT DL+GD I+D
Sbjct: 61  PLSAIGQSEIDVDDFPSLSGRVYGPLCLKRKKLAGIKTTWFPSPNLQTPTTDLTGDHIVD 120

Query: 121 AFNSDVAAH 130
           AFNSDVAAH
Sbjct: 121 AFNSDVAAH 129

[Close]

BLAST of Carg03133 vs. ExPASy TrEMBL
Match: A0A2P5EE91 (Uncharacterized protein OS=Trema orientale OX=63057 GN=TorRG33x02_203870 PE=4 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 3.2e-23
Identity = 68/130 (52.31%), Postives = 87/130 (66.92%), Query Frame = 0


Query: 1   MNRRVQFSHKQRANRTEQFHKYLKPGALAKIRDSRISARLYRYNSLSQIYLRRTASPESS 60
           MNRR +  H+   +RTE F KYLKPGALA+IRDSRISAR +R  SL+QI   R++ P S 
Sbjct: 1   MNRRTRAIHRSSPSRTEPFLKYLKPGALAQIRDSRISARSHRVVSLAQI---RSSQPSSP 60

Query: 61  PLSSIGQ-SEIDVDDFPSLSGRVYGPLCLKRKKFSGIKSTWFPSPNLQTPTADLSGDSII 120
           P S+ GQ S   +D FP  SGRV+GP C +RKK    K+  FPS N  +P  D + D I+
Sbjct: 61  PPSTPGQLSVAAMDGFPCFSGRVFGPRCPQRKKLVASKAVLFPSQNPSSPVLD-AADPIM 120

Query: 121 DAFNSDVAAH 130
           D F++D+AAH
Sbjct: 121 DVFSTDIAAH 126

[Close]

BLAST of Carg03133 vs. ExPASy TrEMBL
Match: A0A7J6GIE5 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_015480 PE=4 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.6e-22
Identity = 70/131 (53.44%), Postives = 87/131 (66.41%), Query Frame = 0


Query: 1   MNRRVQFSHKQRANRTEQFHKYLKPGALAKIRDSRISARLYRYNSLSQIYLRRTASPESS 60
           MNRR +  H+   NR E F KYLKPGALA+IRDSRISAR +R+ S++QI     +SP SS
Sbjct: 1   MNRRTRSLHRSSPNRAEPFLKYLKPGALAQIRDSRISARSHRFLSIAQI----RSSPPSS 60

Query: 61  PLSSIGQSE-IDVDDFPSLSGRVYGPLCLKRKKFSGIKSTWFPSPNLQTPTADLSGDSII 120
           P S+ GQS  I +D FP  SGR++ P C +RKK    KS  FPS N  +P  D + D II
Sbjct: 61  PSSTPGQSTVIAMDGFPCFSGRMFVPRCPQRKKLVASKSVLFPSQNPSSPVLD-AADPII 120

Query: 121 DAF-NSDVAAH 130
           D F +SD+AAH
Sbjct: 121 DVFSSSDIAAH 126

[Close]

BLAST of Carg03133 vs. ExPASy TrEMBL
Match: A0A438EFG1 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=CK203_081763 PE=4 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 4.7e-22
Identity = 59/126 (46.83%), Postives = 81/126 (64.29%), Query Frame = 0


Query: 1   MNRRVQFSHKQRANRTEQFHKYLKPGALAKIRDSRISARLYRYNSLSQIYLRRTASPESS 60
           MNRR++  H+  + R E FH+YLKPGALA++RDSRISAR +R +S SQI+L RT+SP SS
Sbjct: 1   MNRRIRALHRSSSKRDEAFHRYLKPGALAQLRDSRISARSHRVDSHSQIHLHRTSSPPSS 60

Query: 61  PLSSIGQSEIDVDDFPSLSGRVYGPLCLKRKKFSGIKSTWFPSPNLQTPTADLSGDSIID 120
           PL         +D FP  +GR YGP CL+RKK    KS +  S +  +P +D     ++D
Sbjct: 61  PLPP------QIDAFPCFAGRNYGPRCLQRKKLVAAKSVFLKSSSPASPMSDSPDSVVVD 120

Query: 121 AFNSDV 127
            F  ++
Sbjct: 121 LFGPEI 120

[Close]

BLAST of Carg03133 vs. TAIR 10
Match: AT5G54970.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G26960.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 66.6 bits (161), Expect = 1.6e-11
Identity = 45/125 (36.00%), Postives = 69/125 (55.20%), Query Frame = 0


Query: 10  KQRANRTEQFHKYLKPGALAKIRDSRISAR----LYRYNSLSQIYLRRTASPESSPLSSI 69
           K  A R++ FH+YLKPGALA+IR+SRI+AR    L   +SLSQ+    + S  S   ++ 
Sbjct: 6   KSPARRSDGFHRYLKPGALAQIRNSRINARSTTSLVSRSSLSQLVDPISPSENSVDEAAA 65

Query: 70  GQSEIDVDDFPSLSGRVYGPLCLKRKKFSGIKS-TWFPSPNLQTPTADLSGDSIIDAFNS 129
               + +D  P L  ++YGP   +RKK +  +S +     N+  P      +S++   +S
Sbjct: 66  ASRNLTIDQVPHLLRKIYGPYSYQRKKLAAARSVSSMMMMNINPPI-----NSVLVEPSS 125

[Close]

BLAST of Carg03133 vs. TAIR 10
Match: AT4G26960.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G54970.1); Has 22 Blast hits to 22 proteins in 8 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 22; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 59.7 bits (143), Expect = 2.0e-09
Identity = 43/125 (34.40%), Postives = 64/125 (51.20%), Query Frame = 0


Query: 15  RTEQFHKYLKPGALAKIRDSRISARLYRYNSLSQIYLRRTASPESSPLSSIGQS------ 74
           R + FH+Y+KPGALA+IR++RI+      N+ S   L R+ S    P SS  +S      
Sbjct: 40  RNDGFHRYMKPGALAQIRNARIN------NARSNFPLTRSLSDPVDPPSSSSESPIAATP 99

Query: 75  -EIDVDDFPSLSGRVYGPLCLKRKKFSGIKSTWF---PSPNLQTPTADLSGDSIIDAFNS 130
             I +D  P L  ++YGP  + RKK    +S      PSPN  +    ++G+S +   N 
Sbjct: 100 QTITMDQMPHLLSKIYGPYRIGRKKLGPARSAMVKLNPSPN--SILESINGNSNVLNNND 156

The following BLAST results are available for this feature:

BLAST of Carg03133 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022944248.1	5.4e-65	100.00	uncharacterized protein LOC111448755 [Cucurbita moschata] >KAG6570884.1 hypothet...	[more]
XP_023513131.1	6.8e-60	92.25	uncharacterized protein LOC111777660 [Cucurbita pepo subsp. pepo]	[more]
XP_022986202.1	8.3e-58	89.15	uncharacterized protein LOC111484017 [Cucurbita maxima]	[more]
XP_038902395.1	1.2e-45	73.08	uncharacterized protein LOC120089036 [Benincasa hispida]	[more]
PON83836.1	6.7e-23	52.31	hypothetical protein TorRG33x02_203870 [Trema orientale]	[more]

BLAST of Carg03133 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg03133 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FXV4	2.6e-65	100.00	uncharacterized protein LOC111448755 OS=Cucurbita moschata OX=3662 GN=LOC1114487...	[more]
A0A6J1J6X7	4.0e-58	89.15	uncharacterized protein LOC111484017 OS=Cucurbita maxima OX=3661 GN=LOC111484017...	[more]
A0A2P5EE91	3.2e-23	52.31	Uncharacterized protein OS=Trema orientale OX=63057 GN=TorRG33x02_203870 PE=4 SV...	[more]
A0A7J6GIE5	1.6e-22	53.44	Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_015480 PE=4 SV=1	[more]
A0A438EFG1	4.7e-22	46.83	Uncharacterized protein OS=Vitis vinifera OX=29760 GN=CK203_081763 PE=4 SV=1	[more]

BLAST of Carg03133 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54970.1	1.6e-11	36.00	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT4G26960.1	2.0e-09	34.40	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR35495	OS06G0679600 PROTEIN	coord: 1..129
None	No IPR available	PANTHER	PTHR35495:SF10	SUBFAMILY NOT NAMED	coord: 1..129

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg03133-RA	Carg03133-RA	mRNA