Carg03115 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg03115
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionprotein NRT1/ PTR FAMILY 6.4-like
LocationCarg_Chr20: 3042879 .. 3045757 (-)
RNA-Seq ExpressionCarg03115
SyntenyCarg03115
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGCACACAAACAATTCATCTTCTTTCTCTTTCTCTCTCTCTCTCTTTCTCTCTAGCAGAGAGGAAAAAACAAGCAAAAAACAATGGTAACTTGTTTGTGGAGAAACTCCTTAGTTCCTATTGGCTATGAATGGCTTCTTTAACGGTTAAAATCTTCCTTGTTTTCAACAGGTTCTGGTATCTAAGCATGGGAGCGGAGGAGGAGAAGAAGATGCTGCAGTGGATTTTTGGGGAAACCCAGTGGACAAATCAAAAACTGGAGGATGGCTTGCTGCAGGGCTCATCTTAGGTGCTTAATTTTTTCTTTAGTTTTCACAGACCAAGAGTCTAAATCTATCAATTCTAATGCCCAAAATGATGCAGGAACTGAGCTCTCTGAAAGGATATGTGTGATGGGGATATCAATGAACTTAGTGACATATTTAGTTGGGGATTTGCATCTCTCTTCAGCTAAATCTGCAACTATTGTGACTAATTTTCTTGGTGCCCTTAATCTTCTTGGCCTTCTTGGTGGGTTCTTAGCAGATGCCAAGTTGGGACGTTATCTAACTGTTGCAACTTTTGCTTCCATAACTGCTGTGGTAAGTTGTGAGCCATTAAGAATGATTTCAGCAAAGAAATTTCTAGTTCAGAATTTAGCCTAAGCCTTGCTGGTTTTTTGCAGGGTGTTATTTTGTTGACATTGGCCACAACCATCCCTGGCATGCGACCCCCTCATTGTGATGACTCCAGAAGGCAACTTCATCAATGCATTGAAGCTAATGGTGGGCAACTTGCCATGCTCTATGCAGCACTTTATACTATAGCACTCGGCGGAGGGGGGATAAAATCGAACGTTTCAGGATTTGGGTCCGACCAATTCGATGTCAATGATCCTAAGGAGGAGAAGGCCATGATATTCTTCTTCAATAGGTTCTACTTTGCCATAAGCATTGGATCCTTGTTTGCTGTCATTGTGTTGGTTTATGTACAAGACAATGTTGGGAGAGGCTGGGGATATGGAATCTCTGGAGGGACCATGGTGATTGCTGTGATTGTACTGCTTTGTGGGACAACCTTTTACAGGTTTAAGAAGCCTAGAGGAAGTCCTCTAACAGTGATATGGAGGGTGGCTCTTTTGGCTTGGAAGAAGAGAAGTCTCCCTCTCCCATCTCATCCCAGCTTCTTAAATGACTACCAAAATGCCAAGGTTCCTTACACCTACAGGTTCAAGTAAGTGTTTTTCAGATCTATCATCAAATACACTTCGTTTTTTTTTCCCCTCTTTATTGTAAAGAAAACGGTGTTAATGAAAAGGTGAATATCAGGTGTCTTGACAAGGCTGCAATTCTGGACGAGTATGTTGCCAACGATCGAAACAGAAACGATCCATGGATTGTCTCGACCGTGACTGAAGTTGAAGAAGTGAAAATGGTGCTGAAGCTTTTACCTATTTGGTCTACTGGCATCCTCTTTTGGACGATCTACTCTCAAATGACTACGTTCACGGTAGAGCAAGCATCATTTATGGATAGAAAAGTTGGTAGTTTTGAGATTCCTCCTGGTTCTATGTCAGCCTTTCTATTCATCACCATCCTTCTCGTCACTTCCTTGAACGAGAAACTATTCATACCAATTGCACGAAAGCTGACTCACAATGCCCAAGGTCTCACAAGCCTTCAGAGGATTGGAATTGGACTTGTTTTCTCAATTTTTGGCATGGTGGCTGCTGGAGTTGTTGAGAAAGAGAGGAGGACATTAGCTTTGGGACAACGTACTCTAAGTGCTTTCTGGTTAATACCTCAGTTCTTCTTGATTGGTGCTGGAGAGGCATTTACATATGTTGGACAACTCGAGTTTTTTATCAGAGAAGCACCGGAACGAATGAAATCCATGAGCACAGGGCTTTTTCTTAGCACTCTTTCCATGGGATTTTTCGTTAGTAGCTTGTTGGTAACAATCGTGGATAAAGTAACCAACAAGCAATGGCTGAGAAGTAATTTGAACATGGGAAACTTGAACTACTTCTATTGGCTACTTGCAGTGTTGGGGCTGCTAAACTTTTTCTTCTTTCTAGTCCTAGCCCGGAAACATCAGTATAAAGACCAGCATTACTTCAACTCTAAAGACAACAATGAGGAGCTCAAGAGTTCTAATGACTTGGTAGCACTTGAAGTGGAAGGGCCAATAAGCATAAAGGCAACAGAAAGTTAAGAGTGTGTGTTTCAAGGTGTCCCTTGTATAAGTTTAAAAATCCTGCATCTACGTTTGAATATCTCAAAACCTGTAACTACAACCATCCTATGCTTTCAGCATCAATGGCAGATAAGCCACTGCCATTTTGTCTTCCCCTAGGTTCTATATGATCTAAACCATTCAGCATATTTCAAATAATGTTTTCCAGTGCAAACCGGCACTCATTTATGTAAACATCCATGTCTCATAGCAGGCTTCTTACTGAAAGTAGAATCCTCAAATGATTTTGATAGGCATCATTTTCAAACAGGCATCTATTCCAATAAATTATTTTAGCTAGCAACTAGACTCAGCAAGATGTATAGAGTATAAAAGCCTTGATTATCAAATAATTCCAGAAAAGTACACTGCTCAAATATAATCCTTCTCACATCGTCCCGATAAATGAGAAGTAGACCTGATATCTAACAGCAATGCATGTTTCAAGAGGCAGAAGCAGAACTTGGAGAAGGAAGGTGATTTACAACTAATGGAGGTCGGAGGGCCACATGCTGTTCCACCTTCTGTTTACGAGCTTTATACATTTCTTCTGTTTCAACCACTACTAATCGCTTTACCTATAATAAAATTAAGCAAAACAACGAAAAAGGGATCATGGCATTCAGTCACCATGAGGTTGTTCTGTAGTGTGTGCATGTGCGCAG

mRNA sequence

CAGCACACAAACAATTCATCTTCTTTCTCTTTCTCTCTCTCTCTCTTTCTCTCTAGCAGAGAGGAAAAAACAAGCAAAAAACAATGGTAACTTGTTTGTGGAGAAACTCCTTAGTTCCTATTGGCTATGAATGGCTTCTTTAACGGTTAAAATCTTCCTTGTTTTCAACAGGTTCTGGTATCTAAGCATGGGAGCGGAGGAGGAGAAGAAGATGCTGCAGTGGATTTTTGGGGAAACCCAGTGGACAAATCAAAAACTGGAGGATGGCTTGCTGCAGGGCTCATCTTAGGAACTGAGCTCTCTGAAAGGATATGTGTGATGGGGATATCAATGAACTTAGTGACATATTTAGTTGGGGATTTGCATCTCTCTTCAGCTAAATCTGCAACTATTGTGACTAATTTTCTTGGTGCCCTTAATCTTCTTGGCCTTCTTGGTGGGTTCTTAGCAGATGCCAAGTTGGGACGTTATCTAACTGTTGCAACTTTTGCTTCCATAACTGCTGTGGGTGTTATTTTGTTGACATTGGCCACAACCATCCCTGGCATGCGACCCCCTCATTGTGATGACTCCAGAAGGCAACTTCATCAATGCATTGAAGCTAATGGTGGGCAACTTGCCATGCTCTATGCAGCACTTTATACTATAGCACTCGGCGGAGGGGGGATAAAATCGAACGTTTCAGGATTTGGGTCCGACCAATTCGATGTCAATGATCCTAAGGAGGAGAAGGCCATGATATTCTTCTTCAATAGGTTCTACTTTGCCATAAGCATTGGATCCTTGTTTGCTGTCATTGTGTTGGTTTATGTACAAGACAATGTTGGGAGAGGCTGGGGATATGGAATCTCTGGAGGGACCATGGTGATTGCTGTGATTGTACTGCTTTGTGGGACAACCTTTTACAGGTTTAAGAAGCCTAGAGGAAGTCCTCTAACAGTGATATGGAGGGTGGCTCTTTTGGCTTGGAAGAAGAGAAGTCTCCCTCTCCCATCTCATCCCAGCTTCTTAAATGACTACCAAAATGCCAAGGTTCCTTACACCTACAGGTTCAAGTGTCTTGACAAGGCTGCAATTCTGGACGAGTATGTTGCCAACGATCGAAACAGAAACGATCCATGGATTGTCTCGACCGTGACTGAAGTTGAAGAAGTGAAAATGGTGCTGAAGCTTTTACCTATTTGGTCTACTGGCATCCTCTTTTGGACGATCTACTCTCAAATGACTACGTTCACGGTAGAGCAAGCATCATTTATGGATAGAAAAGTTGGTAGTTTTGAGATTCCTCCTGGTTCTATGTCAGCCTTTCTATTCATCACCATCCTTCTCGTCACTTCCTTGAACGAGAAACTATTCATACCAATTGCACGAAAGCTGACTCACAATGCCCAAGGTCTCACAAGCCTTCAGAGGATTGGAATTGGACTTGTTTTCTCAATTTTTGGCATGGTGGCTGCTGGAGTTGTTGAGAAAGAGAGGAGGACATTAGCTTTGGGACAACGTACTCTAAGTGCTTTCTGGTTAATACCTCAGTTCTTCTTGATTGGTGCTGGAGAGGCATTTACATATGTTGGACAACTCGAGTTTTTTATCAGAGAAGCACCGGAACGAATGAAATCCATGAGCACAGGGCTTTTTCTTAGCACTCTTTCCATGGGATTTTTCGTTAGTAGCTTGTTGGTAACAATCGTGGATAAAGTAACCAACAAGCAATGGCTGAGAAGTAATTTGAACATGGGAAACTTGAACTACTTCTATTGGCTACTTGCAGTGTTGGGGCTGCTAAACTTTTTCTTCTTTCTAGTCCTAGCCCGGAAACATCAGTATAAAGACCAGCATTACTTCAACTCTAAAGACAACAATGAGGAGCTCAAGAGTTCTAATGACTTGGTAGCACTTGAAGTGGAAGGGCCAATAAGCATAAAGGCAACAGAAAGTTAAGAGTGTGTGTTTCAAGGTGTCCCTTGTATAAGTTTAAAAATCCTGCATCTACGTTTGAATATCTCAAAACCTGTAACTACAACCATCCTATGCTTTCAGCATCAATGGCAGATAAGCCACTGCCATTTTGTCTTCCCCTAGGTTCTATATGATCTAAACCATTCAGCATATTTCAAATAATGTTTTCCAGTGCAAACCGGCACTCATTTATGTAAACATCCATGTCTCATAGCAGGCTTCTTACTGAAAGTAGAATCCTCAAATGATTTTGATAGGCATCATTTTCAAACAGGCATCTATTCCAATAAATTATTTTAGCTAGCAACTAGACTCAGCAAGATGTATAGAGTATAAAAGCCTTGATTATCAAATAATTCCAGAAAAGTACACTGCTCAAATATAATCCTTCTCACATCGTCCCGATAAATGAGAAGTAGACCTGATATCTAACAGCAATGCATGTTTCAAGAGGCAGAAGCAGAACTTGGAGAAGGAAGGTGATTTACAACTAATGGAGGTCGGAGGGCCACATGCTGTTCCACCTTCTGTTTACGAGCTTTATACATTTCTTCTGTTTCAACCACTACTAATCGCTTTACCTATAATAAAATTAAGCAAAACAACGAAAAAGGGATCATGGCATTCAGTCACCATGAGGTTGTTCTGTAGTGTGTGCATGTGCGCAG

Coding sequence (CDS)

ATGGGGATATCAATGAACTTAGTGACATATTTAGTTGGGGATTTGCATCTCTCTTCAGCTAAATCTGCAACTATTGTGACTAATTTTCTTGGTGCCCTTAATCTTCTTGGCCTTCTTGGTGGGTTCTTAGCAGATGCCAAGTTGGGACGTTATCTAACTGTTGCAACTTTTGCTTCCATAACTGCTGTGGGTGTTATTTTGTTGACATTGGCCACAACCATCCCTGGCATGCGACCCCCTCATTGTGATGACTCCAGAAGGCAACTTCATCAATGCATTGAAGCTAATGGTGGGCAACTTGCCATGCTCTATGCAGCACTTTATACTATAGCACTCGGCGGAGGGGGGATAAAATCGAACGTTTCAGGATTTGGGTCCGACCAATTCGATGTCAATGATCCTAAGGAGGAGAAGGCCATGATATTCTTCTTCAATAGGTTCTACTTTGCCATAAGCATTGGATCCTTGTTTGCTGTCATTGTGTTGGTTTATGTACAAGACAATGTTGGGAGAGGCTGGGGATATGGAATCTCTGGAGGGACCATGGTGATTGCTGTGATTGTACTGCTTTGTGGGACAACCTTTTACAGGTTTAAGAAGCCTAGAGGAAGTCCTCTAACAGTGATATGGAGGGTGGCTCTTTTGGCTTGGAAGAAGAGAAGTCTCCCTCTCCCATCTCATCCCAGCTTCTTAAATGACTACCAAAATGCCAAGGTTCCTTACACCTACAGGTTCAAGTGTCTTGACAAGGCTGCAATTCTGGACGAGTATGTTGCCAACGATCGAAACAGAAACGATCCATGGATTGTCTCGACCGTGACTGAAGTTGAAGAAGTGAAAATGGTGCTGAAGCTTTTACCTATTTGGTCTACTGGCATCCTCTTTTGGACGATCTACTCTCAAATGACTACGTTCACGGTAGAGCAAGCATCATTTATGGATAGAAAAGTTGGTAGTTTTGAGATTCCTCCTGGTTCTATGTCAGCCTTTCTATTCATCACCATCCTTCTCGTCACTTCCTTGAACGAGAAACTATTCATACCAATTGCACGAAAGCTGACTCACAATGCCCAAGGTCTCACAAGCCTTCAGAGGATTGGAATTGGACTTGTTTTCTCAATTTTTGGCATGGTGGCTGCTGGAGTTGTTGAGAAAGAGAGGAGGACATTAGCTTTGGGACAACGTACTCTAAGTGCTTTCTGGTTAATACCTCAGTTCTTCTTGATTGGTGCTGGAGAGGCATTTACATATGTTGGACAACTCGAGTTTTTTATCAGAGAAGCACCGGAACGAATGAAATCCATGAGCACAGGGCTTTTTCTTAGCACTCTTTCCATGGGATTTTTCGTTAGTAGCTTGTTGGTAACAATCGTGGATAAAGTAACCAACAAGCAATGGCTGAGAAGTAATTTGAACATGGGAAACTTGAACTACTTCTATTGGCTACTTGCAGTGTTGGGGCTGCTAAACTTTTTCTTCTTTCTAGTCCTAGCCCGGAAACATCAGTATAAAGACCAGCATTACTTCAACTCTAAAGACAACAATGAGGAGCTCAAGAGTTCTAATGACTTGGTAGCACTTGAAGTGGAAGGGCCAATAAGCATAAAGGCAACAGAAAGTTAA

Protein sequence

MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFYWLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES
Homology
BLAST of Carg03115 vs. NCBI nr
Match: KAG7010750.1 (Protein NRT1/ PTR FAMILY 6.4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1054.3 bits (2725), Expect = 3.6e-304
Identity = 540/540 (100.00%), Postives = 540/540 (100.00%), Query Frame = 0

Query: 1   MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 60
           MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI
Sbjct: 1   MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 60

Query: 61  TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 120
           TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN
Sbjct: 61  TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 120

Query: 121 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG 180
           VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG
Sbjct: 121 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG 180

Query: 181 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVP 240
           TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVP
Sbjct: 181 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVP 240

Query: 241 YTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS 300
           YTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS
Sbjct: 241 YTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS 300

Query: 301 QMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGL 360
           QMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGL
Sbjct: 301 QMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGL 360

Query: 361 TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQ 420
           TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQ
Sbjct: 361 TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQ 420

Query: 421 LEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFY 480
           LEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFY
Sbjct: 421 LEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFY 480

Query: 481 WLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 540
           WLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES
Sbjct: 481 WLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 540

BLAST of Carg03115 vs. NCBI nr
Match: KAG6570905.1 (Protein NRT1/ PTR FAMILY 6.4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1053.9 bits (2724), Expect = 4.7e-304
Identity = 539/540 (99.81%), Postives = 540/540 (100.00%), Query Frame = 0

Query: 1   MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 60
           MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI
Sbjct: 51  MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 110

Query: 61  TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 120
           TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN
Sbjct: 111 TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 170

Query: 121 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG 180
           VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVY+QDNVGRGWGYGISGG
Sbjct: 171 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYIQDNVGRGWGYGISGG 230

Query: 181 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVP 240
           TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVP
Sbjct: 231 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVP 290

Query: 241 YTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS 300
           YTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS
Sbjct: 291 YTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS 350

Query: 301 QMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGL 360
           QMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGL
Sbjct: 351 QMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGL 410

Query: 361 TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQ 420
           TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQ
Sbjct: 411 TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQ 470

Query: 421 LEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFY 480
           LEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFY
Sbjct: 471 LEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFY 530

Query: 481 WLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 540
           WLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES
Sbjct: 531 WLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 590

BLAST of Carg03115 vs. NCBI nr
Match: XP_022944384.1 (protein NRT1/ PTR FAMILY 6.4-like [Cucurbita moschata])

HSP 1 Score: 1052.4 bits (2720), Expect = 1.4e-303
Identity = 539/540 (99.81%), Postives = 540/540 (100.00%), Query Frame = 0

Query: 1   MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 60
           MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI
Sbjct: 51  MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 110

Query: 61  TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 120
           TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN
Sbjct: 111 TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 170

Query: 121 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG 180
           VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG
Sbjct: 171 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG 230

Query: 181 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVP 240
           TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVP
Sbjct: 231 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVP 290

Query: 241 YTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS 300
           YTYRFKCLDKAAILDEYVAN+RNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS
Sbjct: 291 YTYRFKCLDKAAILDEYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS 350

Query: 301 QMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGL 360
           QMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGL
Sbjct: 351 QMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGL 410

Query: 361 TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQ 420
           TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQ
Sbjct: 411 TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQ 470

Query: 421 LEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFY 480
           LEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFY
Sbjct: 471 LEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFY 530

Query: 481 WLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 540
           WLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES
Sbjct: 531 WLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 590

BLAST of Carg03115 vs. NCBI nr
Match: XP_023512951.1 (protein NRT1/ PTR FAMILY 6.4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1047.3 bits (2707), Expect = 4.4e-302
Identity = 537/540 (99.44%), Postives = 537/540 (99.44%), Query Frame = 0

Query: 1   MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 60
           MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI
Sbjct: 51  MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 110

Query: 61  TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 120
           TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN
Sbjct: 111 TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 170

Query: 121 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG 180
           VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG
Sbjct: 171 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG 230

Query: 181 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVP 240
           TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVP
Sbjct: 231 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVP 290

Query: 241 YTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS 300
           YTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS
Sbjct: 291 YTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS 350

Query: 301 QMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGL 360
           QMTTFTVEQASFM RKVGSFEIPPGSMSAFLFI ILLVTSLNEKLFIPIARKLTHNAQGL
Sbjct: 351 QMTTFTVEQASFMGRKVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNAQGL 410

Query: 361 TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQ 420
           TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQ
Sbjct: 411 TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQ 470

Query: 421 LEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFY 480
           LEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLN GNLNYFY
Sbjct: 471 LEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNKGNLNYFY 530

Query: 481 WLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 540
           WLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES
Sbjct: 531 WLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 590

BLAST of Carg03115 vs. NCBI nr
Match: XP_022986273.1 (protein NRT1/ PTR FAMILY 6.4-like [Cucurbita maxima])

HSP 1 Score: 1041.2 bits (2691), Expect = 3.1e-300
Identity = 535/540 (99.07%), Postives = 536/540 (99.26%), Query Frame = 0

Query: 1   MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 60
           MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI
Sbjct: 51  MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 110

Query: 61  TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 120
           TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN
Sbjct: 111 TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 170

Query: 121 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG 180
           VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG
Sbjct: 171 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG 230

Query: 181 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVP 240
           TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLA KKRSLPLPSHPSFLNDYQNAKVP
Sbjct: 231 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLASKKRSLPLPSHPSFLNDYQNAKVP 290

Query: 241 YTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS 300
           YTYRFKCLDKAAILDEYVAND+NRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS
Sbjct: 291 YTYRFKCLDKAAILDEYVANDQNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS 350

Query: 301 QMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGL 360
           QMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFI ILLVTSLNEKLFIPIARKLTHNAQGL
Sbjct: 351 QMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNAQGL 410

Query: 361 TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQ 420
           TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQR LSAFWLIPQFFLIGAGEAFTYVGQ
Sbjct: 411 TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRNLSAFWLIPQFFLIGAGEAFTYVGQ 470

Query: 421 LEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFY 480
           LEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLN GNLNYFY
Sbjct: 471 LEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNKGNLNYFY 530

Query: 481 WLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 540
           WLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES
Sbjct: 531 WLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 590

BLAST of Carg03115 vs. ExPASy Swiss-Prot
Match: Q9LVE0 (Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1)

HSP 1 Score: 751.5 bits (1939), Expect = 6.5e-216
Identity = 379/508 (74.61%), Postives = 424/508 (83.46%), Query Frame = 0

Query: 1   MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 60
           MGISMNLVTYLVGDLH+SSAKSATIVTNF+G LNLLGLLGGFLADAKLGRY  VA  AS+
Sbjct: 51  MGISMNLVTYLVGDLHISSAKSATIVTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASV 110

Query: 61  TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 120
           TA+GV+LLT+ATTI  MRPP CDD RR  HQCIEANG QLA+LY ALYTIALGGGGIKSN
Sbjct: 111 TALGVLLLTVATTISSMRPPICDDFRRLHHQCIEANGHQLALLYVALYTIALGGGGIKSN 170

Query: 121 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG 180
           VSGFGSDQFD +DPKEEK MIFFFNRFYF+IS+GSLFAVI LVYVQDNVGRGWGYGIS  
Sbjct: 171 VSGFGSDQFDTSDPKEEKQMIFFFNRFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAA 230

Query: 181 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVP 240
           TMV+A IVLLCGT  YRFKKP+GSP T IWRV  LAWKKR    P+HPS LN Y N  VP
Sbjct: 231 TMVVAAIVLLCGTKRYRFKKPKGSPFTTIWRVGFLAWKKRKESYPAHPSLLNGYDNTTVP 290

Query: 241 YTYRFKCLDKAAIL---DEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWT 300
           +T   KCLDKAAI        + D    DPWIVSTVT+VEEVK+V+KL+PIW+T ILFWT
Sbjct: 291 HTEMLKCLDKAAISKNESSPSSKDFEEKDPWIVSTVTQVEEVKLVMKLVPIWATNILFWT 350

Query: 301 IYSQMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNA 360
           IYSQMTTFTVEQA+FMDRK+GSF +P GS SAFL +TILL TSLNE++F+P+ R+LT   
Sbjct: 351 IYSQMTTFTVEQATFMDRKLGSFTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKP 410

Query: 361 QGLTSLQRIGIGLVFSIFGMVAAGVVEKERRTLAL-GQRTLSAFWLIPQFFLIGAGEAFT 420
           QG+TSLQRIG+GLVFS+  M  A V+E  RR  A+   + +SAFWL+PQ+FL+GAGEAF 
Sbjct: 411 QGITSLQRIGVGLVFSMAAMAVAAVIENARREAAVNNDKKISAFWLVPQYFLVGAGEAFA 470

Query: 421 YVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNL 480
           YVGQLEFFIREAPERMKSMSTGLFLST+SMGFFVSSLLV++VD+VT+K WLRSNLN   L
Sbjct: 471 YVGQLEFFIREAPERMKSMSTGLFLSTISMGFFVSSLLVSLVDRVTDKSWLRSNLNKARL 530

Query: 481 NYFYWLLAVLGLLNFFFFLVLARKHQYK 505
           NYFYWLL VLG LNF  F+V A KHQYK
Sbjct: 531 NYFYWLLVVLGALNFLIFIVFAMKHQYK 558

BLAST of Carg03115 vs. ExPASy Swiss-Prot
Match: Q05085 (Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 4.5e-140
Identity = 266/525 (50.67%), Postives = 357/525 (68.00%), Query Frame = 0

Query: 1   MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 60
           +GI +NLVTYL G +HL +A +A  VTNFLG   +L LLGGF+AD  LGRYLT+A FA+I
Sbjct: 49  LGIGVNLVTYLTGTMHLGNATAANTVTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAI 108

Query: 61  TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 120
            A GV +LTL+T IPG+RPP C+ +      C +A+G QL +LY ALY  ALG GG+K++
Sbjct: 109 QATGVSILTLSTIIPGLRPPRCNPTTSS--HCEQASGIQLTVLYLALYLTALGTGGVKAS 168

Query: 121 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG 180
           VSGFGSDQFD  +PKE   M +FFNRF+F I++GSL AV VLVYVQD+VGR WGYGI   
Sbjct: 169 VSGFGSDQFDETEPKERSKMTYFFNRFFFCINVGSLLAVTVLVYVQDDVGRKWGYGICAF 228

Query: 181 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQN---- 240
            +V+A+ V L GT  YRFKK  GSP+T +  V + AW+ R L LP+ PS+L D  +    
Sbjct: 229 AIVLALSVFLAGTNRYRFKKLIGSPMTQVAAVIVAAWRNRKLELPADPSYLYDVDDIIAA 288

Query: 241 -------AKVPYTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIW 300
                   K+P+T +F+ LDKAAI D+      N  + W +ST+T+VEEVK ++++LPIW
Sbjct: 289 EGSMKGKQKLPHTEQFRSLDKAAIRDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIW 348

Query: 301 STGILFWTIYSQMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPI 360
           +T ILFWT+++Q+TT +V Q+  +DR +GSFEIPP SM+ F    +LL T++ +++ I +
Sbjct: 349 ATCILFWTVHAQLTTLSVAQSETLDRSIGSFEIPPASMAVFYVGGLLLTTAVYDRVAIRL 408

Query: 361 ARKLTHNAQGLTSLQRIGIGLVFSIFGMVAAGVVEKERRTLA------LGQRTLSAFWLI 420
            +KL +   GL  LQRIG+GL F    M  A +VE +R   A      +    L  + LI
Sbjct: 409 CKKLFNYPHGLRPLQRIGLGLFFGSMAMAVAALVELKRLRTAHAHGPTVKTLPLGFYLLI 468

Query: 421 PQFFLIGAGEAFTYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTN 480
           PQ+ ++G GEA  Y GQL+FF+RE P+ MK MSTGL LSTL++GFF SS+LVTIV+K T 
Sbjct: 469 PQYLIVGIGEALIYTGQLDFFLRECPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTG 528

Query: 481 K--QWLRSNLNMGNLNYFYWLLAVLGLLNFFFFLVLARKHQYKDQ 507
           K   W+  +LN G L  FYWL+AVL  LNF  FLV ++ + YK++
Sbjct: 529 KAHPWIADDLNKGRLYNFYWLVAVLVALNFLIFLVFSKWYVYKEK 571

BLAST of Carg03115 vs. ExPASy Swiss-Prot
Match: Q9SZY4 (Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 3.7e-134
Identity = 261/512 (50.98%), Postives = 358/512 (69.92%), Query Frame = 0

Query: 1   MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 60
           MGI++NLVTYL+  +HL S+ SA IVT+F+G   LL LLGGFLAD+ LGR+ T+  F++I
Sbjct: 46  MGIAVNLVTYLMETMHLPSSTSANIVTDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTI 105

Query: 61  TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 120
            A+G   L +AT +P +RPP C         CI A   Q+ +LY +LY IALG GG+KS+
Sbjct: 106 QALGTGALAVATKLPELRPPTCHHG----EACIPATAFQMTILYVSLYLIALGTGGLKSS 165

Query: 121 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG 180
           +SGFGSDQFD  DPKE+  M FFFNRF+F IS+G+L AV VLVY+QD VGR W YGI   
Sbjct: 166 ISGFGSDQFDDKDPKEKAHMAFFFNRFFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTV 225

Query: 181 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFL--NDYQNAK 240
           +M IA+++ LCGT  YR+KK +GSP+  I++V   A++KR + LP    +L  ++ +  +
Sbjct: 226 SMAIAIVIFLCGTKRYRYKKSQGSPVVQIFQVIAAAFRKRKMELPQSIVYLYEDNPEGIR 285

Query: 241 VPYTYRFKCLDKAAILD----EYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGIL 300
           + +T +F  LDKAAI+     E   +     +PW +S+VT+VEEVKM+++LLPIW+T I+
Sbjct: 286 IEHTDQFHLLDKAAIVAEGDFEQTLDGVAIPNPWKLSSVTKVEEVKMMVRLLPIWATTII 345

Query: 301 FWTIYSQMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLT 360
           FWT Y+QM TF+VEQAS M R +GSF+IP GS++ F    IL+  ++ ++  +P  +K  
Sbjct: 346 FWTTYAQMITFSVEQASTMRRNIGSFKIPAGSLTVFFVAAILITLAVYDRAIMPFWKK-W 405

Query: 361 HNAQGLTSLQRIGIGLVFSIFGMVAAGVVEKERRTLA--LGQRTL--SAFWLIPQFFLIG 420
               G +SLQRI IGLV S  GM AA +VE++R ++A    Q+TL  S F L+PQFFL+G
Sbjct: 406 KGKPGFSSLQRIAIGLVLSTAGMAAAALVEQKRLSVAKSSSQKTLPISVFLLVPQFFLVG 465

Query: 421 AGEAFTYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQ----W 480
           AGEAF Y GQL+FFI ++P+ MK+MSTGLFL+TLS+GFFVSS LV+IV +VT+      W
Sbjct: 466 AGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGW 525

Query: 481 LRSNLNMGNLNYFYWLLAVLGLLNFFFFLVLA 499
           L  N+N G L+YFYWLL +L  +NF  +++ A
Sbjct: 526 LADNINHGRLDYFYWLLVILSGINFVVYIICA 552

BLAST of Carg03115 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 5.5e-114
Identity = 229/523 (43.79%), Postives = 333/523 (63.67%), Query Frame = 0

Query: 2   GISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASIT 61
           G+  NLV YL   L+  +A +A  VTN+ G   +  L+G F+ADA LGRY T+ATF  I 
Sbjct: 47  GMGTNLVNYLESRLNQGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIY 106

Query: 62  AVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNV 121
             G+ LLTL+ ++PG++P +C+            N  Q A+ + ALY IALG GGIK  V
Sbjct: 107 VSGMTLLTLSASVPGLKPGNCN------ADTCHPNSSQTAVFFVALYMIALGTGGIKPCV 166

Query: 122 SGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGT 181
           S FG+DQFD ND  E+     FFN FYF+I++G+L A  VLV++Q NVG GWG+G+    
Sbjct: 167 SSFGADQFDENDENEKIKKSSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVA 226

Query: 182 MVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFL--------ND 241
           MVIAV     G+ FYR ++P GSPLT I++V + A++K S+ +P   S L        N 
Sbjct: 227 MVIAVCFFFFGSRFYRLQRPGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNI 286

Query: 242 YQNAKVPYTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGI 301
             + K+ +T   K  DKAA+  +  +      +PW + +VT+VEE+K ++ LLP+W+TGI
Sbjct: 287 KGSRKLVHTDNLKFFDKAAVESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGI 346

Query: 302 LFWTIYSQMTTFTVEQASFMDRKVG-SFEIPPGSMSAFLFITILLVTSLNEKLFIPIARK 361
           +F T+YSQM+T  V Q + MD+ +G +FEIP  S+S F  +++L  T + ++  IP+ARK
Sbjct: 347 VFATVYSQMSTMFVLQGNTMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARK 406

Query: 362 LTHNAQGLTSLQRIGIGLVFSIFGMVAAGVVE-------KERRTLALGQRTLSAFWLIPQ 421
            T N +G T LQR+GIGLV SIF M+ AGV+E       K        Q  +S FW IPQ
Sbjct: 407 FTRNERGFTQLQRMGIGLVVSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQ 466

Query: 422 FFLIGAGEAFTYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQ 481
           + LIG  E FT++GQLEFF  +AP+ M+S+ + L L+T+++G ++S++LVT+V K+T K 
Sbjct: 467 YLLIGCAEVFTFIGQLEFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKN 526

Query: 482 ----WLRSNLNMGNLNYFYWLLAVLGLLNFFFFLVLARKHQYK 505
               W+  NLN G+L+YF++LLA L  LNF  +L ++++++YK
Sbjct: 527 GKPGWIPDNLNRGHLDYFFYLLATLSFLNFLVYLWISKRYKYK 563

BLAST of Carg03115 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 6.5e-107
Identity = 217/523 (41.49%), Postives = 323/523 (61.76%), Query Frame = 0

Query: 2   GISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASIT 61
           G+S NL+ YL   +++ +  ++  V+N+ G      L+G F+ADA LGRY T+A+F  I 
Sbjct: 48  GMSTNLINYLEKQMNMENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIY 107

Query: 62  AVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNV 121
             G+ LLT++ ++PG+ P  C        +   A  GQ A+ + ALY IALG GGIK  V
Sbjct: 108 IAGMTLLTISASVPGLTPT-CSG------ETCHATAGQTAITFIALYLIALGTGGIKPCV 167

Query: 122 SGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGT 181
           S FG+DQFD  D KE+++   FFN FYF I++G++ A  VLV++Q NVG GWG G+    
Sbjct: 168 SSFGADQFDDTDEKEKESKSSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVA 227

Query: 182 MVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNA---- 241
           M IAV+    G+ FYR +KP GSPLT + +V + + +K  + +P   S L + Q+A    
Sbjct: 228 MAIAVVFFFAGSNFYRLQKPGGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSI 287

Query: 242 ----KVPYTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGI 301
               K+ +T      DKAA+  E       ++  W + TVT+VEE+K +++LLPIW+TGI
Sbjct: 288 IGSRKLEHTKILTFFDKAAVETESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGI 347

Query: 302 LFWTIYSQMTTFTVEQASFMDRKVG-SFEIPPGSMSAFLFITILLVTSLNEKLFIPIARK 361
           +F ++YSQM T  V Q + +D+ +G +F+IP  S+S F  +++L    + +KL +P ARK
Sbjct: 348 VFASVYSQMGTVFVLQGNTLDQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARK 407

Query: 362 LTHNAQGLTSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQR-------TLSAFWLIPQ 421
            T + +G T LQRIGIGLV SIF MV+AG++E  R                ++ FW +PQ
Sbjct: 408 YTGHERGFTQLQRIGIGLVISIFSMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQ 467

Query: 422 FFLIGAGEAFTYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK- 481
           +FL+G  E FT++GQLEFF  +AP+ M+S+ + L L+ ++ G ++S+ LVT+V KVT   
Sbjct: 468 YFLVGCAEVFTFIGQLEFFYDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSG 527

Query: 482 ---QWLRSNLNMGNLNYFYWLLAVLGLLNFFFFLVLARKHQYK 505
               W+  NLN G+L+YF+WLLA L  LNF  +L +A+ + YK
Sbjct: 528 GRPGWIAKNLNNGHLDYFFWLLAGLSFLNFLVYLWIAKWYTYK 563

BLAST of Carg03115 vs. ExPASy TrEMBL
Match: A0A6J1FWL4 (protein NRT1/ PTR FAMILY 6.4-like OS=Cucurbita moschata OX=3662 GN=LOC111448847 PE=3 SV=1)

HSP 1 Score: 1052.4 bits (2720), Expect = 6.6e-304
Identity = 539/540 (99.81%), Postives = 540/540 (100.00%), Query Frame = 0

Query: 1   MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 60
           MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI
Sbjct: 51  MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 110

Query: 61  TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 120
           TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN
Sbjct: 111 TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 170

Query: 121 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG 180
           VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG
Sbjct: 171 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG 230

Query: 181 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVP 240
           TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVP
Sbjct: 231 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVP 290

Query: 241 YTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS 300
           YTYRFKCLDKAAILDEYVAN+RNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS
Sbjct: 291 YTYRFKCLDKAAILDEYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS 350

Query: 301 QMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGL 360
           QMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGL
Sbjct: 351 QMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGL 410

Query: 361 TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQ 420
           TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQ
Sbjct: 411 TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQ 470

Query: 421 LEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFY 480
           LEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFY
Sbjct: 471 LEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFY 530

Query: 481 WLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 540
           WLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES
Sbjct: 531 WLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 590

BLAST of Carg03115 vs. ExPASy TrEMBL
Match: A0A6J1JG19 (protein NRT1/ PTR FAMILY 6.4-like OS=Cucurbita maxima OX=3661 GN=LOC111484070 PE=3 SV=1)

HSP 1 Score: 1041.2 bits (2691), Expect = 1.5e-300
Identity = 535/540 (99.07%), Postives = 536/540 (99.26%), Query Frame = 0

Query: 1   MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 60
           MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI
Sbjct: 51  MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 110

Query: 61  TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 120
           TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN
Sbjct: 111 TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 170

Query: 121 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG 180
           VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG
Sbjct: 171 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG 230

Query: 181 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVP 240
           TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLA KKRSLPLPSHPSFLNDYQNAKVP
Sbjct: 231 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLASKKRSLPLPSHPSFLNDYQNAKVP 290

Query: 241 YTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS 300
           YTYRFKCLDKAAILDEYVAND+NRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS
Sbjct: 291 YTYRFKCLDKAAILDEYVANDQNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS 350

Query: 301 QMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGL 360
           QMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFI ILLVTSLNEKLFIPIARKLTHNAQGL
Sbjct: 351 QMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNAQGL 410

Query: 361 TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQ 420
           TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQR LSAFWLIPQFFLIGAGEAFTYVGQ
Sbjct: 411 TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRNLSAFWLIPQFFLIGAGEAFTYVGQ 470

Query: 421 LEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFY 480
           LEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLN GNLNYFY
Sbjct: 471 LEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNKGNLNYFY 530

Query: 481 WLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 540
           WLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES
Sbjct: 531 WLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 590

BLAST of Carg03115 vs. ExPASy TrEMBL
Match: A0A5D3CCF7 (Protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold757G00100 PE=3 SV=1)

HSP 1 Score: 970.7 bits (2508), Expect = 2.5e-279
Identity = 494/541 (91.31%), Postives = 517/541 (95.56%), Query Frame = 0

Query: 1   MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 60
           MGISMNLVTYLVGDLHL+SAKSATIVTNFLGALNLLGLLGGFLADA+LGRYLTVATFASI
Sbjct: 51  MGISMNLVTYLVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADARLGRYLTVATFASI 110

Query: 61  TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 120
           TAVGVILLTLATTIPGMRPPHCDDS RQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN
Sbjct: 111 TAVGVILLTLATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 170

Query: 121 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG 180
           VSGFGSDQFD NDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQD VGRGWGYGISGG
Sbjct: 171 VSGFGSDQFDTNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGG 230

Query: 181 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVP 240
           TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRV LLAWKKRSLP P+HPSFLNDYQNAKVP
Sbjct: 231 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVP 290

Query: 241 YTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS 300
           YTYRFKCLDKAAI+DEY   D   N+PWIVSTVTE+EEVKMV KL+PIWSTGILFWTIYS
Sbjct: 291 YTYRFKCLDKAAIIDEYATTD--PNNPWIVSTVTEIEEVKMVFKLIPIWSTGILFWTIYS 350

Query: 301 QMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGL 360
           QMTTFTVEQASFM+R+VGSFEIPPGSMSAFLFI ILLVTSLNEKLFIPIARKLTHN QGL
Sbjct: 351 QMTTFTVEQASFMERRVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGL 410

Query: 361 TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRT-LSAFWLIPQFFLIGAGEAFTYVG 420
           TSLQRIGIGLVFSIFGMVAAG VEKER+  A+GQ T +SAFWLIPQFFL+GAGEAF YVG
Sbjct: 411 TSLQRIGIGLVFSIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFAYVG 470

Query: 421 QLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYF 480
           QLEFFIREAPE+MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK+WLRSNLN G+L+YF
Sbjct: 471 QLEFFIREAPEQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKRWLRSNLNKGHLSYF 530

Query: 481 YWLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATE 540
           YWLLAVLGLLNFFFFL+LARKHQYKDQHY +SKDNNEELK SND+VALE+EGPIS+KA E
Sbjct: 531 YWLLAVLGLLNFFFFLLLARKHQYKDQHYVSSKDNNEELKISNDMVALEIEGPISMKAAE 589

BLAST of Carg03115 vs. ExPASy TrEMBL
Match: A0A1S3C263 (protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo OX=3656 GN=LOC103495995 PE=3 SV=1)

HSP 1 Score: 970.7 bits (2508), Expect = 2.5e-279
Identity = 494/541 (91.31%), Postives = 517/541 (95.56%), Query Frame = 0

Query: 1   MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 60
           MGISMNLVTYLVGDLHL+SAKSATIVTNFLGALNLLGLLGGFLADA+LGRYLTVATFASI
Sbjct: 51  MGISMNLVTYLVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADARLGRYLTVATFASI 110

Query: 61  TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 120
           TAVGVILLTLATTIPGMRPPHCDDS RQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN
Sbjct: 111 TAVGVILLTLATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 170

Query: 121 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG 180
           VSGFGSDQFD NDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQD VGRGWGYGISGG
Sbjct: 171 VSGFGSDQFDTNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGG 230

Query: 181 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVP 240
           TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRV LLAWKKRSLP P+HPSFLNDYQNAKVP
Sbjct: 231 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVP 290

Query: 241 YTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS 300
           YTYRFKCLDKAAI+DEY   D   N+PWIVSTVTE+EEVKMV KL+PIWSTGILFWTIYS
Sbjct: 291 YTYRFKCLDKAAIIDEYATTD--PNNPWIVSTVTEIEEVKMVFKLIPIWSTGILFWTIYS 350

Query: 301 QMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGL 360
           QMTTFTVEQASFM+R+VGSFEIPPGSMSAFLFI ILLVTSLNEKLFIPIARKLTHN QGL
Sbjct: 351 QMTTFTVEQASFMERRVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGL 410

Query: 361 TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRT-LSAFWLIPQFFLIGAGEAFTYVG 420
           TSLQRIGIGLVFSIFGMVAAG VEKER+  A+GQ T +SAFWLIPQFFL+GAGEAF YVG
Sbjct: 411 TSLQRIGIGLVFSIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFAYVG 470

Query: 421 QLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYF 480
           QLEFFIREAPE+MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK+WLRSNLN G+L+YF
Sbjct: 471 QLEFFIREAPEQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKRWLRSNLNKGHLSYF 530

Query: 481 YWLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATE 540
           YWLLAVLGLLNFFFFL+LARKHQYKDQHY +SKDNNEELK SND+VALE+EGPIS+KA E
Sbjct: 531 YWLLAVLGLLNFFFFLLLARKHQYKDQHYVSSKDNNEELKISNDMVALEIEGPISMKAAE 589

BLAST of Carg03115 vs. ExPASy TrEMBL
Match: A0A5A7V3P8 (Protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold2406G00170 PE=3 SV=1)

HSP 1 Score: 968.4 bits (2502), Expect = 1.3e-278
Identity = 493/541 (91.13%), Postives = 517/541 (95.56%), Query Frame = 0

Query: 1   MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 60
           MGISMNLVTYLVG+LHL+SAKSATIVTNFLGALNLLGLLGGFLADA+LGRYLTVATFASI
Sbjct: 51  MGISMNLVTYLVGELHLTSAKSATIVTNFLGALNLLGLLGGFLADARLGRYLTVATFASI 110

Query: 61  TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 120
           TAVGVILLTLATTIPGMRPPHCDDS RQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN
Sbjct: 111 TAVGVILLTLATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 170

Query: 121 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG 180
           VSGFGSDQFD NDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQD VGRGWGYGISGG
Sbjct: 171 VSGFGSDQFDTNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGG 230

Query: 181 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVP 240
           TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRV LLAWKKRSLP P+HPSFLNDYQNAKVP
Sbjct: 231 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVP 290

Query: 241 YTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYS 300
           YTYRFKCLDKAAI+DEY   D   N+PWIVSTVTE+EEVKMV KL+PIWSTGILFWTIYS
Sbjct: 291 YTYRFKCLDKAAIIDEYATTD--PNNPWIVSTVTEIEEVKMVFKLIPIWSTGILFWTIYS 350

Query: 301 QMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGL 360
           QMTTFTVEQASFM+R+VGSFEIPPGSMSAFLFI ILLVTSLNEKLFIPIARKLTHN QGL
Sbjct: 351 QMTTFTVEQASFMERRVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGL 410

Query: 361 TSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRT-LSAFWLIPQFFLIGAGEAFTYVG 420
           TSLQRIGIGLVFSIFGMVAAG VEKER+  A+GQ T +SAFWLIPQFFL+GAGEAF YVG
Sbjct: 411 TSLQRIGIGLVFSIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFAYVG 470

Query: 421 QLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYF 480
           QLEFFIREAPE+MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK+WLRSNLN G+L+YF
Sbjct: 471 QLEFFIREAPEQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKRWLRSNLNKGHLSYF 530

Query: 481 YWLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATE 540
           YWLLAVLGLLNFFFFL+LARKHQYKDQHY +SKDNNEELK SND+VALE+EGPIS+KA E
Sbjct: 531 YWLLAVLGLLNFFFFLLLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISMKAAE 589

BLAST of Carg03115 vs. TAIR 10
Match: AT3G21670.1 (Major facilitator superfamily protein )

HSP 1 Score: 751.5 bits (1939), Expect = 4.6e-217
Identity = 379/508 (74.61%), Postives = 424/508 (83.46%), Query Frame = 0

Query: 1   MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 60
           MGISMNLVTYLVGDLH+SSAKSATIVTNF+G LNLLGLLGGFLADAKLGRY  VA  AS+
Sbjct: 51  MGISMNLVTYLVGDLHISSAKSATIVTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASV 110

Query: 61  TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 120
           TA+GV+LLT+ATTI  MRPP CDD RR  HQCIEANG QLA+LY ALYTIALGGGGIKSN
Sbjct: 111 TALGVLLLTVATTISSMRPPICDDFRRLHHQCIEANGHQLALLYVALYTIALGGGGIKSN 170

Query: 121 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG 180
           VSGFGSDQFD +DPKEEK MIFFFNRFYF+IS+GSLFAVI LVYVQDNVGRGWGYGIS  
Sbjct: 171 VSGFGSDQFDTSDPKEEKQMIFFFNRFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAA 230

Query: 181 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVP 240
           TMV+A IVLLCGT  YRFKKP+GSP T IWRV  LAWKKR    P+HPS LN Y N  VP
Sbjct: 231 TMVVAAIVLLCGTKRYRFKKPKGSPFTTIWRVGFLAWKKRKESYPAHPSLLNGYDNTTVP 290

Query: 241 YTYRFKCLDKAAIL---DEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWT 300
           +T   KCLDKAAI        + D    DPWIVSTVT+VEEVK+V+KL+PIW+T ILFWT
Sbjct: 291 HTEMLKCLDKAAISKNESSPSSKDFEEKDPWIVSTVTQVEEVKLVMKLVPIWATNILFWT 350

Query: 301 IYSQMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNA 360
           IYSQMTTFTVEQA+FMDRK+GSF +P GS SAFL +TILL TSLNE++F+P+ R+LT   
Sbjct: 351 IYSQMTTFTVEQATFMDRKLGSFTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKP 410

Query: 361 QGLTSLQRIGIGLVFSIFGMVAAGVVEKERRTLAL-GQRTLSAFWLIPQFFLIGAGEAFT 420
           QG+TSLQRIG+GLVFS+  M  A V+E  RR  A+   + +SAFWL+PQ+FL+GAGEAF 
Sbjct: 411 QGITSLQRIGVGLVFSMAAMAVAAVIENARREAAVNNDKKISAFWLVPQYFLVGAGEAFA 470

Query: 421 YVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNL 480
           YVGQLEFFIREAPERMKSMSTGLFLST+SMGFFVSSLLV++VD+VT+K WLRSNLN   L
Sbjct: 471 YVGQLEFFIREAPERMKSMSTGLFLSTISMGFFVSSLLVSLVDRVTDKSWLRSNLNKARL 530

Query: 481 NYFYWLLAVLGLLNFFFFLVLARKHQYK 505
           NYFYWLL VLG LNF  F+V A KHQYK
Sbjct: 531 NYFYWLLVVLGALNFLIFIVFAMKHQYK 558

BLAST of Carg03115 vs. TAIR 10
Match: AT1G12110.1 (nitrate transporter 1.1 )

HSP 1 Score: 499.6 bits (1285), Expect = 3.2e-141
Identity = 266/525 (50.67%), Postives = 357/525 (68.00%), Query Frame = 0

Query: 1   MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 60
           +GI +NLVTYL G +HL +A +A  VTNFLG   +L LLGGF+AD  LGRYLT+A FA+I
Sbjct: 49  LGIGVNLVTYLTGTMHLGNATAANTVTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAI 108

Query: 61  TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 120
            A GV +LTL+T IPG+RPP C+ +      C +A+G QL +LY ALY  ALG GG+K++
Sbjct: 109 QATGVSILTLSTIIPGLRPPRCNPTTSS--HCEQASGIQLTVLYLALYLTALGTGGVKAS 168

Query: 121 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG 180
           VSGFGSDQFD  +PKE   M +FFNRF+F I++GSL AV VLVYVQD+VGR WGYGI   
Sbjct: 169 VSGFGSDQFDETEPKERSKMTYFFNRFFFCINVGSLLAVTVLVYVQDDVGRKWGYGICAF 228

Query: 181 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQN---- 240
            +V+A+ V L GT  YRFKK  GSP+T +  V + AW+ R L LP+ PS+L D  +    
Sbjct: 229 AIVLALSVFLAGTNRYRFKKLIGSPMTQVAAVIVAAWRNRKLELPADPSYLYDVDDIIAA 288

Query: 241 -------AKVPYTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIW 300
                   K+P+T +F+ LDKAAI D+      N  + W +ST+T+VEEVK ++++LPIW
Sbjct: 289 EGSMKGKQKLPHTEQFRSLDKAAIRDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIW 348

Query: 301 STGILFWTIYSQMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPI 360
           +T ILFWT+++Q+TT +V Q+  +DR +GSFEIPP SM+ F    +LL T++ +++ I +
Sbjct: 349 ATCILFWTVHAQLTTLSVAQSETLDRSIGSFEIPPASMAVFYVGGLLLTTAVYDRVAIRL 408

Query: 361 ARKLTHNAQGLTSLQRIGIGLVFSIFGMVAAGVVEKERRTLA------LGQRTLSAFWLI 420
            +KL +   GL  LQRIG+GL F    M  A +VE +R   A      +    L  + LI
Sbjct: 409 CKKLFNYPHGLRPLQRIGLGLFFGSMAMAVAALVELKRLRTAHAHGPTVKTLPLGFYLLI 468

Query: 421 PQFFLIGAGEAFTYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTN 480
           PQ+ ++G GEA  Y GQL+FF+RE P+ MK MSTGL LSTL++GFF SS+LVTIV+K T 
Sbjct: 469 PQYLIVGIGEALIYTGQLDFFLRECPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTG 528

Query: 481 K--QWLRSNLNMGNLNYFYWLLAVLGLLNFFFFLVLARKHQYKDQ 507
           K   W+  +LN G L  FYWL+AVL  LNF  FLV ++ + YK++
Sbjct: 529 KAHPWIADDLNKGRLYNFYWLVAVLVALNFLIFLVFSKWYVYKEK 571

BLAST of Carg03115 vs. TAIR 10
Match: AT2G26690.1 (Major facilitator superfamily protein )

HSP 1 Score: 479.9 bits (1234), Expect = 2.6e-135
Identity = 261/512 (50.98%), Postives = 358/512 (69.92%), Query Frame = 0

Query: 1   MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 60
           MGI++NLVTYL+  +HL S+ SA IVT+F+G   LL LLGGFLAD+ LGR+ T+  F++I
Sbjct: 46  MGIAVNLVTYLMETMHLPSSTSANIVTDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTI 105

Query: 61  TAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSN 120
            A+G   L +AT +P +RPP C         CI A   Q+ +LY +LY IALG GG+KS+
Sbjct: 106 QALGTGALAVATKLPELRPPTCHHG----EACIPATAFQMTILYVSLYLIALGTGGLKSS 165

Query: 121 VSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGG 180
           +SGFGSDQFD  DPKE+  M FFFNRF+F IS+G+L AV VLVY+QD VGR W YGI   
Sbjct: 166 ISGFGSDQFDDKDPKEKAHMAFFFNRFFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTV 225

Query: 181 TMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFL--NDYQNAK 240
           +M IA+++ LCGT  YR+KK +GSP+  I++V   A++KR + LP    +L  ++ +  +
Sbjct: 226 SMAIAIVIFLCGTKRYRYKKSQGSPVVQIFQVIAAAFRKRKMELPQSIVYLYEDNPEGIR 285

Query: 241 VPYTYRFKCLDKAAILD----EYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGIL 300
           + +T +F  LDKAAI+     E   +     +PW +S+VT+VEEVKM+++LLPIW+T I+
Sbjct: 286 IEHTDQFHLLDKAAIVAEGDFEQTLDGVAIPNPWKLSSVTKVEEVKMMVRLLPIWATTII 345

Query: 301 FWTIYSQMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLT 360
           FWT Y+QM TF+VEQAS M R +GSF+IP GS++ F    IL+  ++ ++  +P  +K  
Sbjct: 346 FWTTYAQMITFSVEQASTMRRNIGSFKIPAGSLTVFFVAAILITLAVYDRAIMPFWKK-W 405

Query: 361 HNAQGLTSLQRIGIGLVFSIFGMVAAGVVEKERRTLA--LGQRTL--SAFWLIPQFFLIG 420
               G +SLQRI IGLV S  GM AA +VE++R ++A    Q+TL  S F L+PQFFL+G
Sbjct: 406 KGKPGFSSLQRIAIGLVLSTAGMAAAALVEQKRLSVAKSSSQKTLPISVFLLVPQFFLVG 465

Query: 421 AGEAFTYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQ----W 480
           AGEAF Y GQL+FFI ++P+ MK+MSTGLFL+TLS+GFFVSS LV+IV +VT+      W
Sbjct: 466 AGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGW 525

Query: 481 LRSNLNMGNLNYFYWLLAVLGLLNFFFFLVLA 499
           L  N+N G L+YFYWLL +L  +NF  +++ A
Sbjct: 526 LADNINHGRLDYFYWLLVILSGINFVVYIICA 552

BLAST of Carg03115 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 412.9 bits (1060), Expect = 3.9e-115
Identity = 229/523 (43.79%), Postives = 333/523 (63.67%), Query Frame = 0

Query: 2   GISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASIT 61
           G+  NLV YL   L+  +A +A  VTN+ G   +  L+G F+ADA LGRY T+ATF  I 
Sbjct: 47  GMGTNLVNYLESRLNQGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIY 106

Query: 62  AVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNV 121
             G+ LLTL+ ++PG++P +C+            N  Q A+ + ALY IALG GGIK  V
Sbjct: 107 VSGMTLLTLSASVPGLKPGNCN------ADTCHPNSSQTAVFFVALYMIALGTGGIKPCV 166

Query: 122 SGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGT 181
           S FG+DQFD ND  E+     FFN FYF+I++G+L A  VLV++Q NVG GWG+G+    
Sbjct: 167 SSFGADQFDENDENEKIKKSSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVA 226

Query: 182 MVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFL--------ND 241
           MVIAV     G+ FYR ++P GSPLT I++V + A++K S+ +P   S L        N 
Sbjct: 227 MVIAVCFFFFGSRFYRLQRPGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNI 286

Query: 242 YQNAKVPYTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGI 301
             + K+ +T   K  DKAA+  +  +      +PW + +VT+VEE+K ++ LLP+W+TGI
Sbjct: 287 KGSRKLVHTDNLKFFDKAAVESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGI 346

Query: 302 LFWTIYSQMTTFTVEQASFMDRKVG-SFEIPPGSMSAFLFITILLVTSLNEKLFIPIARK 361
           +F T+YSQM+T  V Q + MD+ +G +FEIP  S+S F  +++L  T + ++  IP+ARK
Sbjct: 347 VFATVYSQMSTMFVLQGNTMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARK 406

Query: 362 LTHNAQGLTSLQRIGIGLVFSIFGMVAAGVVE-------KERRTLALGQRTLSAFWLIPQ 421
            T N +G T LQR+GIGLV SIF M+ AGV+E       K        Q  +S FW IPQ
Sbjct: 407 FTRNERGFTQLQRMGIGLVVSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQ 466

Query: 422 FFLIGAGEAFTYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQ 481
           + LIG  E FT++GQLEFF  +AP+ M+S+ + L L+T+++G ++S++LVT+V K+T K 
Sbjct: 467 YLLIGCAEVFTFIGQLEFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKN 526

Query: 482 ----WLRSNLNMGNLNYFYWLLAVLGLLNFFFFLVLARKHQYK 505
               W+  NLN G+L+YF++LLA L  LNF  +L ++++++YK
Sbjct: 527 GKPGWIPDNLNRGHLDYFFYLLATLSFLNFLVYLWISKRYKYK 563

BLAST of Carg03115 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 389.4 bits (999), Expect = 4.6e-108
Identity = 217/523 (41.49%), Postives = 323/523 (61.76%), Query Frame = 0

Query: 2   GISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASIT 61
           G+S NL+ YL   +++ +  ++  V+N+ G      L+G F+ADA LGRY T+A+F  I 
Sbjct: 48  GMSTNLINYLEKQMNMENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIY 107

Query: 62  AVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNV 121
             G+ LLT++ ++PG+ P  C        +   A  GQ A+ + ALY IALG GGIK  V
Sbjct: 108 IAGMTLLTISASVPGLTPT-CSG------ETCHATAGQTAITFIALYLIALGTGGIKPCV 167

Query: 122 SGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGT 181
           S FG+DQFD  D KE+++   FFN FYF I++G++ A  VLV++Q NVG GWG G+    
Sbjct: 168 SSFGADQFDDTDEKEKESKSSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVA 227

Query: 182 MVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNA---- 241
           M IAV+    G+ FYR +KP GSPLT + +V + + +K  + +P   S L + Q+A    
Sbjct: 228 MAIAVVFFFAGSNFYRLQKPGGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSI 287

Query: 242 ----KVPYTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGI 301
               K+ +T      DKAA+  E       ++  W + TVT+VEE+K +++LLPIW+TGI
Sbjct: 288 IGSRKLEHTKILTFFDKAAVETESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGI 347

Query: 302 LFWTIYSQMTTFTVEQASFMDRKVG-SFEIPPGSMSAFLFITILLVTSLNEKLFIPIARK 361
           +F ++YSQM T  V Q + +D+ +G +F+IP  S+S F  +++L    + +KL +P ARK
Sbjct: 348 VFASVYSQMGTVFVLQGNTLDQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARK 407

Query: 362 LTHNAQGLTSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQR-------TLSAFWLIPQ 421
            T + +G T LQRIGIGLV SIF MV+AG++E  R                ++ FW +PQ
Sbjct: 408 YTGHERGFTQLQRIGIGLVISIFSMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQ 467

Query: 422 FFLIGAGEAFTYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK- 481
           +FL+G  E FT++GQLEFF  +AP+ M+S+ + L L+ ++ G ++S+ LVT+V KVT   
Sbjct: 468 YFLVGCAEVFTFIGQLEFFYDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSG 527

Query: 482 ---QWLRSNLNMGNLNYFYWLLAVLGLLNFFFFLVLARKHQYK 505
               W+  NLN G+L+YF+WLLA L  LNF  +L +A+ + YK
Sbjct: 528 GRPGWIAKNLNNGHLDYFFWLLAGLSFLNFLVYLWIAKWYTYK 563

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7010750.13.6e-304100.00Protein NRT1/ PTR FAMILY 6.4 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6570905.14.7e-30499.81Protein NRT1/ PTR FAMILY 6.4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022944384.11.4e-30399.81protein NRT1/ PTR FAMILY 6.4-like [Cucurbita moschata][more]
XP_023512951.14.4e-30299.44protein NRT1/ PTR FAMILY 6.4-like [Cucurbita pepo subsp. pepo][more]
XP_022986273.13.1e-30099.07protein NRT1/ PTR FAMILY 6.4-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LVE06.5e-21674.61Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1[more]
Q050854.5e-14050.67Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1[more]
Q9SZY43.7e-13450.98Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1[more]
Q9M3905.5e-11443.79Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Q9LFB86.5e-10741.49Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FWL46.6e-30499.81protein NRT1/ PTR FAMILY 6.4-like OS=Cucurbita moschata OX=3662 GN=LOC111448847 ... [more]
A0A6J1JG191.5e-30099.07protein NRT1/ PTR FAMILY 6.4-like OS=Cucurbita maxima OX=3661 GN=LOC111484070 PE... [more]
A0A5D3CCF72.5e-27991.31Protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3C2632.5e-27991.31protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo OX=3656 GN=LOC103495995 PE=3 SV=1[more]
A0A5A7V3P81.3e-27891.13Protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
Match NameE-valueIdentityDescription
AT3G21670.14.6e-21774.61Major facilitator superfamily protein [more]
AT1G12110.13.2e-14150.67nitrate transporter 1.1 [more]
AT2G26690.12.6e-13550.98Major facilitator superfamily protein [more]
AT3G54140.13.9e-11543.79peptide transporter 1 [more]
AT5G01180.14.6e-10841.49peptide transporter 5 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 51..464
e-value: 3.9E-109
score: 365.1
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 1..515
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 1..510
e-value: 4.4E-169
score: 565.1
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 5..505
NoneNo IPR availablePANTHERPTHR11654:SF373PROTEIN NRT1/ PTR FAMILY 6.4-LIKEcoord: 1..515

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg03115-RACarg03115-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015706 nitrate transport
biological_process GO:0010167 response to nitrate
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015112 nitrate transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity