Carg03056 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg03056
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionMethyltransf_11 domain-containing protein
LocationCarg_Chr09: 3242585 .. 3246122 (+)
RNA-Seq ExpressionCarg03056
SyntenyCarg03056
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATACTTGATGGCTGCACTCCTTGGCAAGCACATGTTCGTCTTCGTTCCATTCCCCACTCGACCCTGTTTCCCACGGCGGTGCTTCTTCAAGTTCGATTCGGCCCTATCCATCTCTCATCGAACTTCTGTTTCAACCGTTCGAGCTACTTCGGCTGCCTCCATGGAAACTAGGACCGAACTATCGGTTTGTATTATCTCTTATCTTCTCTTGCTTGCTCTGTAAATTGAATTGTATAGAATGGAATCTGGATGATTAGGCTCCGGTCGAAGCTGTTTGTAATTTCCTCTGCTTGGTGTTTGAGAGAATCGCAAAAGATATGAGATGATATATGCTAAATGAAATGGCCGATGGTGTGTTGAGGCGTCGTATATATATGTTTTGTTGCGAAAAATCTAGGCGGATTTGAATTGGATTGAGTTATACTGAGAGTTTGAGTGATTCAACTATGTCAATTCTAAACTTCTTTCCGAAGATATAGCCTGGCGTAACGAAACATTATCAGGAACCTCTTCAAGGTTTGAGATCATTGTTTTCAGGTAGTGAGAGAGGAAGTGTAGTTTGAAGTTGGTTCTAGGTCTATTTGATTACATGTTCATTAATTCCTCATGAAAGAAGGATGTTAAAGAATACGTTGGTAAGATTAAATTAGTCAAATGTGTGTCAAGTAAGAGTGACAAGCATTTATTGGGAAAATAAAAGTTGACCTAACCTGTCAACTTTACCTTTCCGAATTGCAATATTCTTTTTTCCTATCTCTAATGATCTTCTGGTTTTTGCGTAGGACCCTGTTTTGATTGATAAGGAAGTCAACAACATTGAGAATATTTTAGCTTGTTCCGTATGTTATGGCCCATTGACTGCGGCAGCTGGTTCGGGACTTTCAGTGTATGTTTGAAACTGTATTGTACCTATTTTTGCTTTTCTTATTTCTTCTATTGCGTCTTAGTAATTGATGTGTCTTTATTTATATGCTTTTGATACAGGGAGTCCACAAAGGGATATCAATTGGAATGCGGCACATGTAAAAAGTCATTCACTGGCAGTGAATCTCACTTGGACTTGACCATATCCGCTGGAATTAACAATGAAGAATCCATGCCTGTTACCACAGAGATTTTTCGGTATTTCACTGTTGTCATCTGCTGTGTATTATGAAGGGGCAGTTAATTAGCATGCTACTAAAATTTTCAAATGCAAAAGGCTTCATGGTCTTGAGAGAAATTAATTTTGTTACAAATTCCATGTGATGGATCTTTATGCTTCATGTTAAAAGGCATTTTTGTTTAGAGCTAGAAGCTCGGTAAGCCATATACACGGGGACACCATTGCTACAATAAAAATTGACGGAATCCAATATACACGGGGACATATTGGTGTACCTATCAGAGGTTTATGGAGGTCTGGGATATTTTCAGAAATATTTTTGATTTTTGCCATATTGGAATTAGGATTATATTGGTAGCTAGCTAGTCTTAGTTGTAATGAAACAAATGAAGTTATGGGTGTTTGGTTGGAACTTGTGATACCTCTCCCTCGTTGCCTCCTTCCCCTTTTTTCTCATCTCATGTTTATCAGCTTTTCACTGGATCAGGACTAGATTGGTGTCTTTTCTGTATGAGAGGGGCTGGCGTCAAAGTTTTTCAGCAATCCTTGGATTTCCAGGCCCAGAAAAGGAGGTGATGTCAGCTCTTTTATCTGTCATGTTATTAAACTGCCTACATGGTACTTTTTATGTAATATCAGCATGTAAGTATAGGCTCAACATTAGGAACTGGATTAGTCCATTAATACCAATGCAAAGTCAGAGGCAGGTCTAGTATTTTGTTTTAGAGTGTTTTATTACTGATGAAGAGGAGGCCCTAGTGCCAACCCCTATAACAATGTTGAGGTCCGCAAAGATTAGTGATTTTAACGTGTCTCTGTTTTCACGCCTACTAGACTAGTGGTATCAGTTCAAGATGTAACCATGTTCCCAATACTAAGGACATGTTGATTTCATTGCAGTTCGAGTTTATAAAAAATTTCATCACACCAGTCTTAGGTGGAAACATAATTGATGCAAGTTGTGGAAGCGGAATGTTTTCAAGAATCTTTGCCAAGAGTGGGTTATTTTCTTCTGTTGTTGCATTGGACTACTCGGAGAACATGTTAAAGGAGTGTTATGCATTCATCAAAGAAGAGGAAAACTTCCCCAAAGAGTAAACAATATTTTCTTTTAGTTCTAGCTTTACATTAGTAGTTGTGGAATTGCTATAGTTCAAGGATCTTTGTGTTGCATATTTAGATTGTTGTTTGAGTTTTTAATCTAAATGAAAATTGTCATTCTTAGGAGCTTGGTTCTGATTAGAGCTGATATTGCTCGGCTTCCATTTGCTTCAAGTTCTATTGATGCAGTGCATGCTGGTGCTGCTTTACACTGCTGGCCTTCACCATCAGCTGCTGTGAGTATTTTTCATCTGTATCTGGGGGTTATAGGTTGTGATTTGACGTTTCATAGTTGCACTAATGAAAAACACACTGCACAAGCTGCAGTTTGTTGATGGTTATTGGCTTTTTACTATAGTGATATGATTACTCATCTTCAAAGAAAATATATTTTACTTCTCTTTACTTCGCTTGTATGAGTGTTAAATGTGTATATTACTGGCACCAACATATACCTATAATTGTACCACAGATTCATCTCACTAAACTAATCAACGGATATGCTTTTGTGATTAACTGTGTCCAGAATGGTCTTGCAGGTGGCTGAAATAAGTAGAATTTTAAAACCCGGAGGAGTATTTGTTGGGAGCACTTACATAATCGATGGACCATTTTCTTTTATGCCATTTTTGGGGACTCAAATAGAGGTATATTCGCAATTTGTTTTAATTTTCCCCTCTACAACTTCTGGGATTCTCCTTGGATGCTTAGGTAGTTTGATTACTTCAGTTCATTATATATATACACTTTTCACTCTTCGAAATCGCTCCACGTAAGTAGTGTGAAAGTTCTCTATTTATAATCATTTAAAAAATCAAATAAATTACAAAAAGATATAATAGGAAGAGCAATAAATGGATACAAAATATTTGTATGTAATAAAATGGTAAATATGATACCTACATGAGTCGTAATGGAAGATATTTGCCTGTAATAAAAGATAAAAAATTCTATATATAGTAAATTATAATTCATGACTAAACATACTATAAAACACAGTGTTTACCATGGCTTAATGCTGCAACGTTGCATCCAAATTCTGGTTTTTTGTTCCTGTTTTTGCCTCTTACCTTAGTAGTTAATCCTTAAACTTTGCATTGTGTGTATTGTGCATCTCTCTCCGCACAGGTTGCAAAGAAGACAATAGGGGGCCACATCGTTCTATCTGAACGTGAATTAGAAGAACTCTGCACGGCCTGTGGTTTGGTCGACTTTAGATGTTTAAGAAATAGGAGGTTTGTAATGTTCTCTGCTAGAAAACGCAGCTGAACATTTGGGTTGTGGTTTCCAGTCCTCTGGTTTTATTATAATATATATATATATATATATATG

mRNA sequence

ATACTTGATGGCTGCACTCCTTGGCAAGCACATGTTCGTCTTCGTTCCATTCCCCACTCGACCCTGTTTCCCACGGCGGTGCTTCTTCAAGTTCGATTCGGCCCTATCCATCTCTCATCGAACTTCTGTTTCAACCGTTCGAGCTACTTCGGCTGCCTCCATGGAAACTAGGACCGAACTATCGGACCCTGTTTTGATTGATAAGGAAGTCAACAACATTGAGAATATTTTAGCTTGTTCCGTATGTTATGGCCCATTGACTGCGGCAGCTGGTTCGGGACTTTCAGTGGAGTCCACAAAGGGATATCAATTGGAATGCGGCACATGTAAAAAGTCATTCACTGGCAGTGAATCTCACTTGGACTTGACCATATCCGCTGGAATTAACAATGAAGAATCCATGCCTGTTACCACAGAGATTTTTCGGACTAGATTGGTGTCTTTTCTGTATGAGAGGGGCTGGCGTCAAAGTTTTTCAGCAATCCTTGGATTTCCAGGCCCAGAAAAGGAGTTCGAGTTTATAAAAAATTTCATCACACCAGTCTTAGGTGGAAACATAATTGATGCAAGTTGTGGAAGCGGAATGTTTTCAAGAATCTTTGCCAAGAGTGGGTTATTTTCTTCTGTTGTTGCATTGGACTACTCGGAGAACATGTTAAAGGAGTGTTATGCATTCATCAAAGAAGAGGAAAACTTCCCCAAAGAGAGCTTGGTTCTGATTAGAGCTGATATTGCTCGGCTTCCATTTGCTTCAAGTTCTATTGATGCAGTGCATGCTGGTGCTGCTTTACACTGCTGGCCTTCACCATCAGCTGCTGTGGCTGAAATAAGTAGAATTTTAAAACCCGGAGGAGTATTTGTTGGGAGCACTTACATAATCGATGGACCATTTTCTTTTATGCCATTTTTGGGGACTCAAATAGAGGTTGCAAAGAAGACAATAGGGGGCCACATCGTTCTATCTGAACGTGAATTAGAAGAACTCTGCACGGCCTGTGGTTTGGTCGACTTTAGATGTTTAAGAAATAGGAGGTTTGTAATGTTCTCTGCTAGAAAACGCAGCTGAACATTTGGGTTGTGGTTTCCAGTCCTCTGGTTTTATTATAATATATATATATATATATATATG

Coding sequence (CDS)

ATGGCTGCACTCCTTGGCAAGCACATGTTCGTCTTCGTTCCATTCCCCACTCGACCCTGTTTCCCACGGCGGTGCTTCTTCAAGTTCGATTCGGCCCTATCCATCTCTCATCGAACTTCTGTTTCAACCGTTCGAGCTACTTCGGCTGCCTCCATGGAAACTAGGACCGAACTATCGGACCCTGTTTTGATTGATAAGGAAGTCAACAACATTGAGAATATTTTAGCTTGTTCCGTATGTTATGGCCCATTGACTGCGGCAGCTGGTTCGGGACTTTCAGTGGAGTCCACAAAGGGATATCAATTGGAATGCGGCACATGTAAAAAGTCATTCACTGGCAGTGAATCTCACTTGGACTTGACCATATCCGCTGGAATTAACAATGAAGAATCCATGCCTGTTACCACAGAGATTTTTCGGACTAGATTGGTGTCTTTTCTGTATGAGAGGGGCTGGCGTCAAAGTTTTTCAGCAATCCTTGGATTTCCAGGCCCAGAAAAGGAGTTCGAGTTTATAAAAAATTTCATCACACCAGTCTTAGGTGGAAACATAATTGATGCAAGTTGTGGAAGCGGAATGTTTTCAAGAATCTTTGCCAAGAGTGGGTTATTTTCTTCTGTTGTTGCATTGGACTACTCGGAGAACATGTTAAAGGAGTGTTATGCATTCATCAAAGAAGAGGAAAACTTCCCCAAAGAGAGCTTGGTTCTGATTAGAGCTGATATTGCTCGGCTTCCATTTGCTTCAAGTTCTATTGATGCAGTGCATGCTGGTGCTGCTTTACACTGCTGGCCTTCACCATCAGCTGCTGTGGCTGAAATAAGTAGAATTTTAAAACCCGGAGGAGTATTTGTTGGGAGCACTTACATAATCGATGGACCATTTTCTTTTATGCCATTTTTGGGGACTCAAATAGAGGTTGCAAAGAAGACAATAGGGGGCCACATCGTTCTATCTGAACGTGAATTAGAAGAACTCTGCACGGCCTGTGGTTTGGTCGACTTTAGATGTTTAAGAAATAGGAGGTTTGTAATGTTCTCTGCTAGAAAACGCAGCTGA

Protein sequence

MAALLGKHMFVFVPFPTRPCFPRRCFFKFDSALSISHRTSVSTVRATSAASMETRTELSDPVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDLTISAGINNEESMPVTTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVLGGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPFLGTQIEVAKKTIGGHIVLSERELEELCTACGLVDFRCLRNRRFVMFSARKRS
Homology
BLAST of Carg03056 vs. NCBI nr
Match: KAG7024628.1 (putative methyltransferase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 699.1 bits (1803), Expect = 1.9e-197
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0

Query: 1   MAALLGKHMFVFVPFPTRPCFPRRCFFKFDSALSISHRTSVSTVRATSAASMETRTELSD 60
           MAALLGKHMFVFVPFPTRPCFPRRCFFKFDSALSISHRTSVSTVRATSAASMETRTELSD
Sbjct: 1   MAALLGKHMFVFVPFPTRPCFPRRCFFKFDSALSISHRTSVSTVRATSAASMETRTELSD 60

Query: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120
           PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL
Sbjct: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120

Query: 121 TISAGINNEESMPVTTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180
           TISAGINNEESMPVTTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL
Sbjct: 121 TISAGINNEESMPVTTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180

Query: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240
           GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA
Sbjct: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240

Query: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300
           DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF
Sbjct: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300

Query: 301 LGTQIEVAKKTIGGHIVLSERELEELCTACGLVDFRCLRNRRFVMFSARKRS 353
           LGTQIEVAKKTIGGHIVLSERELEELCTACGLVDFRCLRNRRFVMFSARKRS
Sbjct: 301 LGTQIEVAKKTIGGHIVLSERELEELCTACGLVDFRCLRNRRFVMFSARKRS 352

BLAST of Carg03056 vs. NCBI nr
Match: XP_022937076.1 (uncharacterized methyltransferase At1g78140, chloroplastic [Cucurbita moschata])

HSP 1 Score: 694.9 bits (1792), Expect = 3.6e-196
Identity = 348/352 (98.86%), Postives = 351/352 (99.72%), Query Frame = 0

Query: 1   MAALLGKHMFVFVPFPTRPCFPRRCFFKFDSALSISHRTSVSTVRATSAASMETRTELSD 60
           MAALLGKHMFVFVPFPTRPCFPRRCFF+FDSALSISHRTSVSTVRATSAASMETRTELSD
Sbjct: 1   MAALLGKHMFVFVPFPTRPCFPRRCFFEFDSALSISHRTSVSTVRATSAASMETRTELSD 60

Query: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120
           PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL
Sbjct: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120

Query: 121 TISAGINNEESMPVTTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180
           TISAGINNEESMP TTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL
Sbjct: 121 TISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180

Query: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240
           GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA
Sbjct: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240

Query: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300
           DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF
Sbjct: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300

Query: 301 LGTQIEVAKKTIGGHIVLSERELEELCTACGLVDFRCLRNRRFVMFSARKRS 353
           LGTQIE+AKKTIGGHIVLSERELEELCTACG+VDFRCLRNRRFVMFSARKRS
Sbjct: 301 LGTQIEIAKKTIGGHIVLSERELEELCTACGMVDFRCLRNRRFVMFSARKRS 352

BLAST of Carg03056 vs. NCBI nr
Match: XP_023535731.1 (uncharacterized methyltransferase At1g78140, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 687.6 bits (1773), Expect = 5.8e-194
Identity = 345/352 (98.01%), Postives = 347/352 (98.58%), Query Frame = 0

Query: 1   MAALLGKHMFVFVPFPTRPCFPRRCFFKFDSALSISHRTSVSTVRATSAASMETRTELSD 60
           MAALLGKHMFVFVPFPTRPCFPRRCFFKFDSALSISHR SVSTVRATSAASMETRTELSD
Sbjct: 1   MAALLGKHMFVFVPFPTRPCFPRRCFFKFDSALSISHRASVSTVRATSAASMETRTELSD 60

Query: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120
           PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL
Sbjct: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120

Query: 121 TISAGINNEESMPVTTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180
           TISAGINNEESMP TTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL
Sbjct: 121 TISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180

Query: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240
           GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA
Sbjct: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240

Query: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300
           DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF
Sbjct: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300

Query: 301 LGTQIEVAKKTIGGHIVLSERELEELCTACGLVDFRCLRNRRFVMFSARKRS 353
           LGTQIE+AKK IGGHIVLSE ELEELC ACG+VDFRCLRNRRFVMFSARKRS
Sbjct: 301 LGTQIEIAKKIIGGHIVLSESELEELCMACGMVDFRCLRNRRFVMFSARKRS 352

BLAST of Carg03056 vs. NCBI nr
Match: XP_022976042.1 (uncharacterized methyltransferase At1g78140, chloroplastic-like [Cucurbita maxima] >XP_022976043.1 uncharacterized methyltransferase At1g78140, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 686.4 bits (1770), Expect = 1.3e-193
Identity = 344/352 (97.73%), Postives = 347/352 (98.58%), Query Frame = 0

Query: 1   MAALLGKHMFVFVPFPTRPCFPRRCFFKFDSALSISHRTSVSTVRATSAASMETRTELSD 60
           MAALLGKHMFVFVPFPTRPCFP+ CFFKFDSALSISHR SVSTVRATSA SMETRTELSD
Sbjct: 1   MAALLGKHMFVFVPFPTRPCFPQPCFFKFDSALSISHRASVSTVRATSATSMETRTELSD 60

Query: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120
           PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL
Sbjct: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120

Query: 121 TISAGINNEESMPVTTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180
           TISAGINNEESMP TTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL
Sbjct: 121 TISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180

Query: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240
           GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA
Sbjct: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240

Query: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300
           DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF
Sbjct: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300

Query: 301 LGTQIEVAKKTIGGHIVLSERELEELCTACGLVDFRCLRNRRFVMFSARKRS 353
           LGTQIE+AKK IGGHIVLSERELEELCTACG+VDFRCLRNRRFVMFSARKRS
Sbjct: 301 LGTQIEIAKKIIGGHIVLSERELEELCTACGMVDFRCLRNRRFVMFSARKRS 352

BLAST of Carg03056 vs. NCBI nr
Match: KAG6591745.1 (putative methyltransferase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 679.5 bits (1752), Expect = 1.6e-191
Identity = 343/344 (99.71%), Postives = 343/344 (99.71%), Query Frame = 0

Query: 9   MFVFVPFPTRPCFPRRCFFKFDSALSISHRTSVSTVRATSAASMETRTELSDPVLIDKEV 68
           MFVFVPFPTRPCFPRRCFFKFDSALSISHRTSVSTVRATSAASMETRTELSDPVLIDKEV
Sbjct: 1   MFVFVPFPTRPCFPRRCFFKFDSALSISHRTSVSTVRATSAASMETRTELSDPVLIDKEV 60

Query: 69  NNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDLTISAGINN 128
           NNIENILACSVCYGPLTAAA SGLSVESTKGYQLECGTCKKSFTGSESHLDLTISAGINN
Sbjct: 61  NNIENILACSVCYGPLTAAACSGLSVESTKGYQLECGTCKKSFTGSESHLDLTISAGINN 120

Query: 129 EESMPVTTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVLGGNIIDAS 188
           EESMPVTTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVLGGNIIDAS
Sbjct: 121 EESMPVTTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVLGGNIIDAS 180

Query: 189 CGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRADIARLPFA 248
           CGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRADIARLPFA
Sbjct: 181 CGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRADIARLPFA 240

Query: 249 SSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPFLGTQIEVA 308
           SSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPFLGTQIEVA
Sbjct: 241 SSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPFLGTQIEVA 300

Query: 309 KKTIGGHIVLSERELEELCTACGLVDFRCLRNRRFVMFSARKRS 353
           KKTIGGHIVLSERELEELCTACGLVDFRCLRNRRFVMFSARKRS
Sbjct: 301 KKTIGGHIVLSERELEELCTACGLVDFRCLRNRRFVMFSARKRS 344

BLAST of Carg03056 vs. ExPASy Swiss-Prot
Match: Q8LBV4 (Uncharacterized methyltransferase At1g78140, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g78140 PE=1 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 9.5e-99
Identity = 185/318 (58.18%), Postives = 239/318 (75.16%), Query Frame = 0

Query: 40  SVSTVRATSAASMETRTELSDPVLIDKEVNN--IENILACSVCYGPLT-AAAGSGLSVES 99
           S S   ++++ S+ET +  +   +I+K+  N   + ILAC +CY  L   +  +GL   +
Sbjct: 39  SRSAHASSASVSVETNSNSNVDFVIEKKDKNRGEKKILACPICYNSLAWISQPNGLIESA 98

Query: 100 TKGYQLECGTCKKSFTGSESHLDLTISAGINN-EESMPVTTEIFRTRLVSFLYERGWRQS 159
             G Q++C TCK+S++G+E+HLDL +++G     E MP++TE+FRT LVSFLYERGWRQ+
Sbjct: 99  ASGIQVQCNTCKRSYSGNETHLDLAVASGSKRYSEPMPLSTELFRTPLVSFLYERGWRQN 158

Query: 160 FSAILGFPGPEKEFEFIKNFITPVLGGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSEN 219
           F    GFPGPEKEFE  K ++ PVLGGNIIDASCGSGMFSR+F +S LFS V+ALDYSEN
Sbjct: 159 F-IWGGFPGPEKEFEMAKAYLKPVLGGNIIDASCGSGMFSRLFTRSDLFSLVIALDYSEN 218

Query: 220 MLKECYAFIKEEENFP-KESLVLIRADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEI 279
           ML++CY  + +EENFP KE LVL+RADIARLPF S S+DAVHAGAALHCWPSPS+AVAEI
Sbjct: 219 MLRQCYELLNKEENFPNKEKLVLVRADIARLPFLSGSVDAVHAGAALHCWPSPSSAVAEI 278

Query: 280 SRILKPGGVFVGSTYIIDGPFSFMPFLGTQIEVAKKTIGGHIVLSERELEELCTACGLVD 339
           SR+L+PGGVFV +T+I DGPFSF+PFL    +   +  G HI L+ERELE++C ACGLV+
Sbjct: 279 SRVLRPGGVFVATTFIYDGPFSFIPFLKNLRQEIMRYSGSHIFLNERELEDICKACGLVN 338

Query: 340 FRCLRNRRFVMFSARKRS 353
           F  +RN  F+M SA K S
Sbjct: 339 FTRVRNGPFIMLSATKPS 355

BLAST of Carg03056 vs. ExPASy Swiss-Prot
Match: Q0WPT7 (Uncharacterized methyltransferase At2g41040, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g41040 PE=1 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 6.4e-71
Identity = 145/345 (42.03%), Postives = 216/345 (62.61%), Query Frame = 0

Query: 18  RPCFPRR-CFFKFDSALSISHRTSVSTVR------ATSAASMETRTELSDPVLIDKEVNN 77
           +P  P R  FF  DS   +   ++ ++VR      A SA + ++    ++   I+ E   
Sbjct: 18  KPFLPNRSSFFSSDSQSPLLRFSASTSVRSRFPSAAISAVAPKSDINKNETPKIEIEET- 77

Query: 78  IENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDLTISAGINN-E 137
              + AC VCY PL     SG+++++      +CG C K+++  + +LDLT++A +++  
Sbjct: 78  --QVFACPVCYEPLMRKGPSGINLQAIYRSGFKCGQCNKTYSSKDEYLDLTVTADLDDYN 137

Query: 138 ESMPVTTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVLGGNIIDASC 197
           E  P+TTE+FR+ LVSFLYERGWRQ+F    GFPGP++EF   + +     GG ++D SC
Sbjct: 138 EVKPITTELFRSPLVSFLYERGWRQAFKR-SGFPGPDEEFRMAEEYFKEAEGGLLVDVSC 197

Query: 198 GSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKE-SLVLIRADIARLPFA 257
           GSG+FSR FA+SG +S V+ALDYSENML++C  FIK +  F    ++ ++RAD++RLPF 
Sbjct: 198 GSGLFSRKFAQSGKYSGVIALDYSENMLRQCKEFIKNDNTFDNSTNIAVVRADVSRLPFP 257

Query: 258 SSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGST---YIIDGPFSFMPFLGTQI 317
           S S+DAVHAGAALHCWPSP+ A+AEI R+L+ GGVFVG+T   Y    P+   PF     
Sbjct: 258 SGSVDAVHAGAALHCWPSPTNAIAEICRVLRSGGVFVGTTFLRYSPSTPWIIRPF----- 317

Query: 318 EVAKKTIGGHIVLSERELEELCTACGLVDFRCLRNRRFVMFSARK 351
               + +  +  L + E++++CT+CGL D+       F+MF+ARK
Sbjct: 318 --QSRILQSYNYLMQDEIKDVCTSCGLTDYEDYIQDSFIMFTARK 351

BLAST of Carg03056 vs. ExPASy Swiss-Prot
Match: Q2YY85 (Demethylmenaquinone methyltransferase OS=Staphylococcus aureus (strain bovine RF122 / ET3-1) OX=273036 GN=menG PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 7.7e-08
Identity = 36/106 (33.96%), Postives = 57/106 (53.77%), Query Frame = 0

Query: 181 GGNIIDASCGSGMFSRIFAKS-GLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIR 240
           G   +D  CG+G ++   +K+ G    V  +D+SENML+     + +E+    E++ L+ 
Sbjct: 50  GTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLE-----VGKEKTASMENVKLVH 109

Query: 241 ADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFV 286
            D   LPF  +S D V  G  L   P    A+ E++R+LKPGG+ V
Sbjct: 110 GDAMELPFGDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVV 150

BLAST of Carg03056 vs. ExPASy Swiss-Prot
Match: A7X2H6 (Demethylmenaquinone methyltransferase OS=Staphylococcus aureus (strain Mu3 / ATCC 700698) OX=418127 GN=menG PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.0e-07
Identity = 36/106 (33.96%), Postives = 57/106 (53.77%), Query Frame = 0

Query: 181 GGNIIDASCGSGMFSRIFAKS-GLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIR 240
           G   +D  CG+G ++   +K+ G    V  +D+SENML+     + +E+    E++ L+ 
Sbjct: 50  GTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLE-----VGKEKTASMENVKLVH 109

Query: 241 ADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFV 286
            D   LPF  +S D V  G  L   P    A+ E++R+LKPGG+ V
Sbjct: 110 GDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVV 150

BLAST of Carg03056 vs. ExPASy Swiss-Prot
Match: A6U1T9 (Demethylmenaquinone methyltransferase OS=Staphylococcus aureus (strain JH1) OX=359787 GN=menG PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.0e-07
Identity = 36/106 (33.96%), Postives = 57/106 (53.77%), Query Frame = 0

Query: 181 GGNIIDASCGSGMFSRIFAKS-GLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIR 240
           G   +D  CG+G ++   +K+ G    V  +D+SENML+     + +E+    E++ L+ 
Sbjct: 50  GTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLE-----VGKEKTASMENVKLVH 109

Query: 241 ADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFV 286
            D   LPF  +S D V  G  L   P    A+ E++R+LKPGG+ V
Sbjct: 110 GDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVV 150

BLAST of Carg03056 vs. ExPASy TrEMBL
Match: A0A6J1F9C0 (uncharacterized methyltransferase At1g78140, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111443486 PE=4 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 1.7e-196
Identity = 348/352 (98.86%), Postives = 351/352 (99.72%), Query Frame = 0

Query: 1   MAALLGKHMFVFVPFPTRPCFPRRCFFKFDSALSISHRTSVSTVRATSAASMETRTELSD 60
           MAALLGKHMFVFVPFPTRPCFPRRCFF+FDSALSISHRTSVSTVRATSAASMETRTELSD
Sbjct: 1   MAALLGKHMFVFVPFPTRPCFPRRCFFEFDSALSISHRTSVSTVRATSAASMETRTELSD 60

Query: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120
           PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL
Sbjct: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120

Query: 121 TISAGINNEESMPVTTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180
           TISAGINNEESMP TTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL
Sbjct: 121 TISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180

Query: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240
           GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA
Sbjct: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240

Query: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300
           DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF
Sbjct: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300

Query: 301 LGTQIEVAKKTIGGHIVLSERELEELCTACGLVDFRCLRNRRFVMFSARKRS 353
           LGTQIE+AKKTIGGHIVLSERELEELCTACG+VDFRCLRNRRFVMFSARKRS
Sbjct: 301 LGTQIEIAKKTIGGHIVLSERELEELCTACGMVDFRCLRNRRFVMFSARKRS 352

BLAST of Carg03056 vs. ExPASy TrEMBL
Match: A0A6J1IKY5 (uncharacterized methyltransferase At1g78140, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476562 PE=4 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 6.2e-194
Identity = 344/352 (97.73%), Postives = 347/352 (98.58%), Query Frame = 0

Query: 1   MAALLGKHMFVFVPFPTRPCFPRRCFFKFDSALSISHRTSVSTVRATSAASMETRTELSD 60
           MAALLGKHMFVFVPFPTRPCFP+ CFFKFDSALSISHR SVSTVRATSA SMETRTELSD
Sbjct: 1   MAALLGKHMFVFVPFPTRPCFPQPCFFKFDSALSISHRASVSTVRATSATSMETRTELSD 60

Query: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120
           PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL
Sbjct: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120

Query: 121 TISAGINNEESMPVTTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180
           TISAGINNEESMP TTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL
Sbjct: 121 TISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180

Query: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240
           GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA
Sbjct: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240

Query: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300
           DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF
Sbjct: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300

Query: 301 LGTQIEVAKKTIGGHIVLSERELEELCTACGLVDFRCLRNRRFVMFSARKRS 353
           LGTQIE+AKK IGGHIVLSERELEELCTACG+VDFRCLRNRRFVMFSARKRS
Sbjct: 301 LGTQIEIAKKIIGGHIVLSERELEELCTACGMVDFRCLRNRRFVMFSARKRS 352

BLAST of Carg03056 vs. ExPASy TrEMBL
Match: A0A1S3CQA4 (uncharacterized methyltransferase At1g78140, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503586 PE=4 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.2e-149
Identity = 273/346 (78.90%), Postives = 301/346 (86.99%), Query Frame = 0

Query: 10  FVFVPFPTRPCFPRRCFFKFDSALSISHRTSVSTVRATSAASMETRTEL---SDPVLIDK 69
           F+ +P PT+     RCFF F S LSIS R S+ST+R++SA  METRT +   SD V+IDK
Sbjct: 11  FLPLPLPTQFSSFTRCFFNFHSTLSISPRLSLSTLRSSSAPPMETRTNIIPPSDSVMIDK 70

Query: 70  EVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDLTISAGI 129
           EVN I+NILACS+C GPLTAAA SGLSVEST GYQLECGTCKKSFTGSESHLDLTI+ GI
Sbjct: 71  EVNKIKNILACSICRGPLTAAADSGLSVESTNGYQLECGTCKKSFTGSESHLDLTITGGI 130

Query: 130 NNEESMPVTTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVLGGNIID 189
           N+ ESMP  TEIFRTRLVSFLYERGWRQSFS +LGFPGPEKEFE IKNFITPVLGG+IID
Sbjct: 131 NSGESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFITPVLGGSIID 190

Query: 190 ASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRADIARLP 249
           ASCGSGMFSRIFAKSGLFSSVVALDYSENML++CY FIK+EENFP E LVLIRADIARLP
Sbjct: 191 ASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCYGFIKQEENFPNERLVLIRADIARLP 250

Query: 250 FASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPFLGTQIE 309
           FASSS+DAVHAGAA+HCWPSPSA VAEISRIL+PGGVFV ST+I+DGPFSF+PFL  QIE
Sbjct: 251 FASSSVDAVHAGAAVHCWPSPSAGVAEISRILRPGGVFVASTFIMDGPFSFVPFLRIQIE 310

Query: 310 VAKKTIGGHIVLSERELEELCTACGLVDFRCLRNRRFVMFSARKRS 353
             ++  G  I LSERELEELCTACGLVDF+CLRNR+FVM SA KRS
Sbjct: 311 GIQQIAGSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATKRS 356

BLAST of Carg03056 vs. ExPASy TrEMBL
Match: A0A6J1D0A7 (uncharacterized methyltransferase At1g78140, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111016077 PE=4 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 2.0e-147
Identity = 277/357 (77.59%), Postives = 309/357 (86.55%), Query Frame = 0

Query: 1   MAALLGKHMFV-FVPFPTRPCFPRR-CFFKFDS-ALSISHRTSVSTVRATSAASMETRTE 60
           MAA++ KHM + FV  PTR CF RR CFF FDS  LSIS R S STV A+S ASM+TR +
Sbjct: 1   MAAVVAKHMLLAFV--PTRLCFFRRCCFFNFDSPTLSISTRISASTVPASSIASMDTRLQ 60

Query: 61  LSDPVLIDKEVNNIENILACSVCYGPLT-AAAGSGLSVESTKGYQLECGTCKKSFTGSES 120
            SD  ++D+EVNNIENILACSVCYGPLT AAAGSGLSVEST GYQLECGTC+K+FTGS +
Sbjct: 61  PSDSSILDREVNNIENILACSVCYGPLTAAAAGSGLSVESTNGYQLECGTCRKTFTGSGT 120

Query: 121 HLDLTISAGINN-EESMPVTTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNF 180
           HLDLTI++G NN  + MP +TEIFRTRLVSFLYERGWRQSFS  LGFPGPE EFE I+ F
Sbjct: 121 HLDLTIASGNNNYGDPMPASTEIFRTRLVSFLYERGWRQSFSVALGFPGPETEFELIRTF 180

Query: 181 ITPVLGGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESL 240
           +TP+LGGNIIDASCGSG+FSRIFAKSGLFSSVVALDYSENMLK+CY FI++EENFP+E+L
Sbjct: 181 MTPILGGNIIDASCGSGLFSRIFAKSGLFSSVVALDYSENMLKQCYEFIQQEENFPRENL 240

Query: 241 VLIRADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPF 300
           VLIRADIARLPFAS+S+DAVHAGAALHCWPSPSAAVAEISRIL+PGGVFV ST+I DGPF
Sbjct: 241 VLIRADIARLPFASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIADGPF 300

Query: 301 SFMPFLGTQIEVAKKTIGGHIVLSERELEELCTACGLVDFRCLRNRRFVMFSARKRS 353
           SF+PFLGTQ E  K+  G HIVLSERELEELC ACGLV FRC+RNR FVM SA K S
Sbjct: 301 SFIPFLGTQRERVKQITGSHIVLSERELEELCRACGLVGFRCVRNRLFVMISATKPS 355

BLAST of Carg03056 vs. ExPASy TrEMBL
Match: A0A5J5B623 (Methyltransf_11 domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_027362 PE=4 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 5.9e-112
Identity = 213/335 (63.58%), Postives = 261/335 (77.91%), Query Frame = 0

Query: 23  RRCFFK--FDSALSISHRTSVSTVRATSA-ASMETRTELSDPVLIDK-EVNNIENILACS 82
           RR FFK  F  +     R S + VRA+S+ A +ET+    D +L +K EV+  +NILAC 
Sbjct: 25  RRRFFKPLFAPSPPTFERMSAAKVRASSSTAFVETK---PDSILDEKVEVSTSKNILACP 84

Query: 83  VCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDLTISAGINN-EESMPVTTE 142
           +CY PL      G SV+S      +C TCKKS+ G+E+HLDLT+++G     ESMPV+T+
Sbjct: 85  ICYVPLIWNGNPGFSVDSAPQTSFQCSTCKKSYFGNETHLDLTVTSGAKEYGESMPVSTQ 144

Query: 143 IFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVLGGNIIDASCGSGMFSRI 202
           IFR  LVSFLYERGWRQSFS + GFPGPEKEFE  KN++ PVLGGNI+DASCGSG+FSR+
Sbjct: 145 IFRFSLVSFLYERGWRQSFSVLGGFPGPEKEFELTKNYLKPVLGGNIVDASCGSGLFSRL 204

Query: 203 FAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRADIARLPFASSSIDAVHA 262
           FAKSGLFS V+ALDYSENML++CY FIK+EE+FPKE+L+L+RADI+RLPFASSS+DAVHA
Sbjct: 205 FAKSGLFSLVIALDYSENMLRQCYEFIKQEESFPKENLILVRADISRLPFASSSVDAVHA 264

Query: 263 GAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPFLGTQIEVAKKTIGGHIV 322
           GAALHCWPSPSAAVAEISR+L+PGGVFV +TY++DGPF+ +P L    E   +  G HI 
Sbjct: 265 GAALHCWPSPSAAVAEISRVLRPGGVFVATTYLLDGPFAALPLLRPLRENIGQISGSHIF 324

Query: 323 LSERELEELCTACGLVDFRCLRNRRFVMFSARKRS 353
           L+ERELE+LC ACGLV F C+RNRRFVM SARK S
Sbjct: 325 LAERELEDLCRACGLVGFTCVRNRRFVMISARKAS 356

BLAST of Carg03056 vs. TAIR 10
Match: AT1G78140.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 361.7 bits (927), Expect = 6.7e-100
Identity = 185/318 (58.18%), Postives = 239/318 (75.16%), Query Frame = 0

Query: 40  SVSTVRATSAASMETRTELSDPVLIDKEVNN--IENILACSVCYGPLT-AAAGSGLSVES 99
           S S   ++++ S+ET +  +   +I+K+  N   + ILAC +CY  L   +  +GL   +
Sbjct: 39  SRSAHASSASVSVETNSNSNVDFVIEKKDKNRGEKKILACPICYNSLAWISQPNGLIESA 98

Query: 100 TKGYQLECGTCKKSFTGSESHLDLTISAGINN-EESMPVTTEIFRTRLVSFLYERGWRQS 159
             G Q++C TCK+S++G+E+HLDL +++G     E MP++TE+FRT LVSFLYERGWRQ+
Sbjct: 99  ASGIQVQCNTCKRSYSGNETHLDLAVASGSKRYSEPMPLSTELFRTPLVSFLYERGWRQN 158

Query: 160 FSAILGFPGPEKEFEFIKNFITPVLGGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSEN 219
           F    GFPGPEKEFE  K ++ PVLGGNIIDASCGSGMFSR+F +S LFS V+ALDYSEN
Sbjct: 159 F-IWGGFPGPEKEFEMAKAYLKPVLGGNIIDASCGSGMFSRLFTRSDLFSLVIALDYSEN 218

Query: 220 MLKECYAFIKEEENFP-KESLVLIRADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEI 279
           ML++CY  + +EENFP KE LVL+RADIARLPF S S+DAVHAGAALHCWPSPS+AVAEI
Sbjct: 219 MLRQCYELLNKEENFPNKEKLVLVRADIARLPFLSGSVDAVHAGAALHCWPSPSSAVAEI 278

Query: 280 SRILKPGGVFVGSTYIIDGPFSFMPFLGTQIEVAKKTIGGHIVLSERELEELCTACGLVD 339
           SR+L+PGGVFV +T+I DGPFSF+PFL    +   +  G HI L+ERELE++C ACGLV+
Sbjct: 279 SRVLRPGGVFVATTFIYDGPFSFIPFLKNLRQEIMRYSGSHIFLNERELEDICKACGLVN 338

Query: 340 FRCLRNRRFVMFSARKRS 353
           F  +RN  F+M SA K S
Sbjct: 339 FTRVRNGPFIMLSATKPS 355

BLAST of Carg03056 vs. TAIR 10
Match: AT2G41040.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 269.2 bits (687), Expect = 4.5e-72
Identity = 145/345 (42.03%), Postives = 216/345 (62.61%), Query Frame = 0

Query: 18  RPCFPRR-CFFKFDSALSISHRTSVSTVR------ATSAASMETRTELSDPVLIDKEVNN 77
           +P  P R  FF  DS   +   ++ ++VR      A SA + ++    ++   I+ E   
Sbjct: 18  KPFLPNRSSFFSSDSQSPLLRFSASTSVRSRFPSAAISAVAPKSDINKNETPKIEIEET- 77

Query: 78  IENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDLTISAGINN-E 137
              + AC VCY PL     SG+++++      +CG C K+++  + +LDLT++A +++  
Sbjct: 78  --QVFACPVCYEPLMRKGPSGINLQAIYRSGFKCGQCNKTYSSKDEYLDLTVTADLDDYN 137

Query: 138 ESMPVTTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVLGGNIIDASC 197
           E  P+TTE+FR+ LVSFLYERGWRQ+F    GFPGP++EF   + +     GG ++D SC
Sbjct: 138 EVKPITTELFRSPLVSFLYERGWRQAFKR-SGFPGPDEEFRMAEEYFKEAEGGLLVDVSC 197

Query: 198 GSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKE-SLVLIRADIARLPFA 257
           GSG+FSR FA+SG +S V+ALDYSENML++C  FIK +  F    ++ ++RAD++RLPF 
Sbjct: 198 GSGLFSRKFAQSGKYSGVIALDYSENMLRQCKEFIKNDNTFDNSTNIAVVRADVSRLPFP 257

Query: 258 SSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGST---YIIDGPFSFMPFLGTQI 317
           S S+DAVHAGAALHCWPSP+ A+AEI R+L+ GGVFVG+T   Y    P+   PF     
Sbjct: 258 SGSVDAVHAGAALHCWPSPTNAIAEICRVLRSGGVFVGTTFLRYSPSTPWIIRPF----- 317

Query: 318 EVAKKTIGGHIVLSERELEELCTACGLVDFRCLRNRRFVMFSARK 351
               + +  +  L + E++++CT+CGL D+       F+MF+ARK
Sbjct: 318 --QSRILQSYNYLMQDEIKDVCTSCGLTDYEDYIQDSFIMFTARK 351

BLAST of Carg03056 vs. TAIR 10
Match: AT1G20330.1 (sterol methyltransferase 2 )

HSP 1 Score: 49.3 bits (116), Expect = 7.4e-06
Identity = 46/176 (26.14%), Postives = 74/176 (42.05%), Query Frame = 0

Query: 126 INNEESMPVTTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKE------FEFIKNFITPV 185
           I   E +P   + F   LV+ +YE GW QSF      PG   +       E   + I   
Sbjct: 64  IETAEKVPDFVDTF-YNLVTDIYEWGWGQSFHFSPSIPGKSHKDATRLHEEMAVDLIQVK 123

Query: 186 LGGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIR 245
            G  I+D  CG G   R  A S   ++VV +  +E  +    A +  ++        ++ 
Sbjct: 124 PGQKILDVGCGVGGPMRAIA-SHSRANVVGITINEYQVNR--ARLHNKKAGLDALCEVVC 183

Query: 246 ADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPF 296
            +  ++PF  +S D  ++  A    P      AEI R+LKPG ++V   ++    F
Sbjct: 184 GNFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKF 235

BLAST of Carg03056 vs. TAIR 10
Match: AT5G13710.1 (sterol methyltransferase 1 )

HSP 1 Score: 48.1 bits (113), Expect = 1.6e-05
Identity = 52/193 (26.94%), Postives = 78/193 (40.41%), Query Frame = 0

Query: 112 TGSESHLDLTISAGINNEESMPVTTEIFRT--RLVSFLYERGWRQSFSAILGFPGPEKEF 171
           T  E +    +  G N EE     T++      L +  YE GW +SF     + G E   
Sbjct: 18  TAVEKYEQYHVFHGGNEEERKANYTDMVNKYYDLATSFYEYGWGESFHFAQRWKG-ESLR 77

Query: 172 EFIK---NFITPVL----GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYA 231
           E IK   +F+   L    G  ++D  CG G   R  A+   FS+ V    + N  +    
Sbjct: 78  ESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIAR---FSNSVVTGLNNNEYQITRG 137

Query: 232 FIKEEENFPKESLVLIRADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGG 291
                     ++   ++AD  ++PF  +S DAV+A  A    P       EI R+LKPG 
Sbjct: 138 KELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYAIEATCHAPDAYGCYKEIYRVLKPGQ 197

Query: 292 VFVGSTYIIDGPF 296
            F    + +   F
Sbjct: 198 CFAAYEWCMTDAF 206

BLAST of Carg03056 vs. TAIR 10
Match: AT5G13710.2 (sterol methyltransferase 1 )

HSP 1 Score: 48.1 bits (113), Expect = 1.6e-05
Identity = 52/193 (26.94%), Postives = 78/193 (40.41%), Query Frame = 0

Query: 112 TGSESHLDLTISAGINNEESMPVTTEIFRT--RLVSFLYERGWRQSFSAILGFPGPEKEF 171
           T  E +    +  G N EE     T++      L +  YE GW +SF     + G E   
Sbjct: 18  TAVEKYEQYHVFHGGNEEERKANYTDMVNKYYDLATSFYEYGWGESFHFAQRWKG-ESLR 77

Query: 172 EFIK---NFITPVL----GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYA 231
           E IK   +F+   L    G  ++D  CG G   R  A+   FS+ V    + N  +    
Sbjct: 78  ESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIAR---FSNSVVTGLNNNEYQITRG 137

Query: 232 FIKEEENFPKESLVLIRADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGG 291
                     ++   ++AD  ++PF  +S DAV+A  A    P       EI R+LKPG 
Sbjct: 138 KELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYAIEATCHAPDAYGCYKEIYRVLKPGQ 197

Query: 292 VFVGSTYIIDGPF 296
            F    + +   F
Sbjct: 198 CFAAYEWCMTDAF 206

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7024628.11.9e-197100.00putative methyltransferase, chloroplastic [Cucurbita argyrosperma subsp. argyros... [more]
XP_022937076.13.6e-19698.86uncharacterized methyltransferase At1g78140, chloroplastic [Cucurbita moschata][more]
XP_023535731.15.8e-19498.01uncharacterized methyltransferase At1g78140, chloroplastic [Cucurbita pepo subsp... [more]
XP_022976042.11.3e-19397.73uncharacterized methyltransferase At1g78140, chloroplastic-like [Cucurbita maxim... [more]
KAG6591745.11.6e-19199.71putative methyltransferase, chloroplastic, partial [Cucurbita argyrosperma subsp... [more]
Match NameE-valueIdentityDescription
Q8LBV49.5e-9958.18Uncharacterized methyltransferase At1g78140, chloroplastic OS=Arabidopsis thalia... [more]
Q0WPT76.4e-7142.03Uncharacterized methyltransferase At2g41040, chloroplastic OS=Arabidopsis thalia... [more]
Q2YY857.7e-0833.96Demethylmenaquinone methyltransferase OS=Staphylococcus aureus (strain bovine RF... [more]
A7X2H61.0e-0733.96Demethylmenaquinone methyltransferase OS=Staphylococcus aureus (strain Mu3 / ATC... [more]
A6U1T91.0e-0733.96Demethylmenaquinone methyltransferase OS=Staphylococcus aureus (strain JH1) OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1F9C01.7e-19698.86uncharacterized methyltransferase At1g78140, chloroplastic OS=Cucurbita moschata... [more]
A0A6J1IKY56.2e-19497.73uncharacterized methyltransferase At1g78140, chloroplastic-like OS=Cucurbita max... [more]
A0A1S3CQA41.2e-14978.90uncharacterized methyltransferase At1g78140, chloroplastic OS=Cucumis melo OX=36... [more]
A0A6J1D0A72.0e-14777.59uncharacterized methyltransferase At1g78140, chloroplastic OS=Momordica charanti... [more]
A0A5J5B6235.9e-11263.58Methyltransf_11 domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_0... [more]
Match NameE-valueIdentityDescription
AT1G78140.16.7e-10058.18S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT2G41040.14.5e-7242.03S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G20330.17.4e-0626.14sterol methyltransferase 2 [more]
AT5G13710.11.6e-0526.94sterol methyltransferase 1 [more]
AT5G13710.21.6e-0526.94sterol methyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013216Methyltransferase type 11PFAMPF08241Methyltransf_11coord: 186..285
e-value: 5.9E-21
score: 74.9
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 139..348
e-value: 3.8E-30
score: 107.0
NoneNo IPR availablePANTHERPTHR43591METHYLTRANSFERASEcoord: 37..351
NoneNo IPR availablePANTHERPTHR43591:SF46OS08G0411200 PROTEINcoord: 37..351
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 183..291
e-value: 2.66571E-11
score: 57.825
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 145..343

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg03056-RACarg03056-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0006744 ubiquinone biosynthetic process
molecular_function GO:0008168 methyltransferase activity