Carg02988 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg02988
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	isoamylase 3, chloroplastic isoform X2
Location	Carg_Chr09: 2837826 .. 2839632 (+)
RNA-Seq Expression	Carg02988
Synteny	Carg02988

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTATTGGCCTTTTGCTGGTATGCATAGGAGGATTTGACTCTATAATCTGCATTTAGTACTTTTCCTCGCCTTCCCTCCCAAAAGAACAAAAATAGATGGGAGAAAAATGAACCGGCAGGTCTTAGAGAACAGGTTTGTCAAAGATGTGTATATGTATACTCACTTAACGGATATCAAGCCATGGAACTTTATGCTATGTTTAGCCCACCTTCTTGGGACTGGCTTCATGTAGATGTGCACAAAATTGGTCATGCAAACTGTTTTGGGAATCTATGTTCTATGCTTTGAAGATTTCTAGTATTTACAGGCTTCATGATGGTAATGGAGAAGACGTTTACTTGGCATTCAATGCTCATGATTATTTTGTCAGTGTTTCATTACCCTCTCCTCCAACAAATCGAAAATGGTTCCGTGTGGTTAGTTTCTCTCTTGCCATTTTGTTGCTTCTAATTTCTGTCCAACTTCCATTGAGTTGCTTTAGGCTTGAATTAACTTGGTAGAACTGCATATTTTTCAAACCGTGTTCGTGAATTAGTTCTTACGGGTTTTCGCTTCTCATGCAGGTGGACACCAATCTGGAGTCTCCCCAAGACTTTGTATTGGATGGCATTCGGGGCATTGGAAGCACCTATAATTTGGCTCCCTATTCCTCAATTCTTCTGAAAGCCAAGTTAGAAAATGAACTTCTTTGATACTAACTCTATATTTAATCAAACCATTATTAGGCAGATGAATCAAGTTCTGTACAACATGACAATCGAAGTATCGTGGAAGAAATAAATTATGCAATCTAATGGAGGACGTGTAGAGCTATCCTCGGCATGGAATTCAGGTACCTGTTTTATGTTTATAGCATATTGGGAAAGAATTTTCTTTTTCTTCTGAAACAGATTAGAGTGTTGTTCATTGTTGCAATTACTTTCAACAATTAGATATAAGTGAATAAAATTATGATCATTAGGTATCTATTTTAGAAGGTCTTTTTTATGATTTTTGTTACTTTCGTCTTTGACCGATTGAAATGCATCCTTCTAAAATAAATGGAAGTCTTAGCAAACTTCCTTTTCTCAAGAAACTTGATCTTGATTCATGTAGAAACTCGATCTTGATTCGTGTAGAAACTCAAGATCTATGGTCCTAGTTGAAATAAAGAGAACAAAGTTCATACCGGTATAATTTCTTAGTTGAGTGAAATACCAGAGAATTATTAGGTACCATACGAGCAGGTCTGACAAAAATCTCTGTATAACCTCCTCAATTTCTCCATAAAAGAGCTAGATTAATTTTAACCCTCGGTATTGAAACAAAATTGTTAGGAATACAAACATTATCATTCATTAGTTAGAAGTGTCCTCACAAGGTTTATGAGAGTTTTGCTTGGACGAATATTTGAATATGAAACAAAAAATGATGAGCCTCGATTTCTTAGCAATATGGTTTGGGTGATAAAGATCATAGCCACTACTCTTCTTTCTAATAGAAAGATTACGAGGATAGCTCGAGATAGCTCAAGGGACCTTTATGATGTGATAAAGAGTGGTCTTCATCAACCATTTCACATTCATGGATTGAACTCTAATAGAATATTCTCCGAACAAAAGAAAAATAGAATTAGAACAAATTCTTAACCTAGAAAAACTGGATGGTTATTGTTTGGTACTTTGTTCAACGACAAAATTATAAACCGTCCGGTGAAATTTATGTTAGTATTATGTCAAATAAAATGAATACAATAATATTAGATAGTAATCATATGGAAATCTAGACTACAACAACAGCCTACAGCGCTACAGGTTATATATA

mRNA sequence

ATGCCTATTGGCCTTTTGCTGCCCACCTTCTTGGGACTGGCTTCATGTAGATGTGCACAAAATTGGTCATGCAAACTGTTTTGGGAATCTATGTTCTATGCTTTGAAGATTTCTAGTATTTACAGGCTTCATGATGGTAATGGAGAAGACGTTTACTTGGCATTCAATGCTCATGATTATTTTGTCAGTGTTTCATTACCCTCTCCTCCAACAAATCGAAAATGGTTCCGTGTGGTGGACACCAATCTGGAGTCTCCCCAAGACTTTGTATTGGATGGCATTCGGGGCATTGGAAGCACCTATAATTTGGCTCCCTATTCCTCAATTCTTCTGAAAGCCAAGTTAGAAAATGAACTTCTTTGATACTAACTCTATATTTAATCAAACCATTATTAGGCAGATGAATCAAGTTCTGTACAACATGACAATCGAAGTATCGTGGAAGAAATAAATTATGCAATCTAATGGAGGACGTGTAGAGCTATCCTCGGCATGGAATTCAGGTACCTGTTTTATGTTTATAGCATATTGGGAAAGAATTTTCTTTTTCTTCTGAAACAGATTAGAGTGTTGTTCATTGTTGCAATTACTTTCAACAATTAGATATAAGTGAATAAAATTATGATCATTAGGTATCTATTTTAGAAGGTCTTTTTTATGATTTTTGTTACTTTCGTCTTTGACCGATTGAAATGCATCCTTCTAAAATAAATGGAAGTCTTAGCAAACTTCCTTTTCTCAAGAAACTTGATCTTGATTCATGTAGAAACTCGATCTTGATTCGTGTAGAAACTCAAGATCTATGGTCCTAGTTGAAATAAAGAGAACAAAGTTCATACCGGTATAATTTCTTAGTTGAGTGAAATACCAGAGAATTATTAGGTACCATACGAGCAGGTCTGACAAAAATCTCTGTATAACCTCCTCAATTTCTCCATAAAAGAGCTAGATTAATTTTAACCCTCGGTATTGAAACAAAATTGTTAGGAATACAAACATTATCATTCATTAGTTAGAAGTGTCCTCACAAGGTTTATGAGAGTTTTGCTTGGACGAATATTTGAATATGAAACAAAAAATGATGAGCCTCGATTTCTTAGCAATATGGTTTGGGTGATAAAGATCATAGCCACTACTCTTCTTTCTAATAGAAAGATTACGAGGATAGCTCGAGATAGCTCAAGGGACCTTTATGATGTGATAAAGAGTGGTCTTCATCAACCATTTCACATTCATGGATTGAACTCTAATAGAATATTCTCCGAACAAAAGAAAAATAGAATTAGAACAAATTCTTAACCTAGAAAAACTGGATGGTTATTGTTTGGTACTTTGTTCAACGACAAAATTATAAACCGTCCGGTGAAATTTATGTTAGTATTATGTCAAATAAAATGAATACAATAATATTAGATAGTAATCATATGGAAATCTAGACTACAACAACAGCCTACAGCGCTACAGGTTATATATA

Coding sequence (CDS)

ATGCCTATTGGCCTTTTGCTGCCCACCTTCTTGGGACTGGCTTCATGTAGATGTGCACAAAATTGGTCATGCAAACTGTTTTGGGAATCTATGTTCTATGCTTTGAAGATTTCTAGTATTTACAGGCTTCATGATGGTAATGGAGAAGACGTTTACTTGGCATTCAATGCTCATGATTATTTTGTCAGTGTTTCATTACCCTCTCCTCCAACAAATCGAAAATGGTTCCGTGTGGTGGACACCAATCTGGAGTCTCCCCAAGACTTTGTATTGGATGGCATTCGGGGCATTGGAAGCACCTATAATTTGGCTCCCTATTCCTCAATTCTTCTGAAAGCCAAGTTAGAAAATGAACTTCTTTGA

Protein sequence

MPIGLLLPTFLGLASCRCAQNWSCKLFWESMFYALKISSIYRLHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGSTYNLAPYSSILLKAKLENELL

Homology

[Close]

BLAST of Carg02988 vs. NCBI nr
Match: KAG7024561.1 (Isoamylase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 253.8 bits (647), Expect = 7.2e-64
Identity = 120/120 (100.00%), Postives = 120/120 (100.00%), Query Frame = 0


Query: 1   MPIGLLLPTFLGLASCRCAQNWSCKLFWESMFYALKISSIYRLHDGNGEDVYLAFNAHDY 60
           MPIGLLLPTFLGLASCRCAQNWSCKLFWESMFYALKISSIYRLHDGNGEDVYLAFNAHDY
Sbjct: 1   MPIGLLLPTFLGLASCRCAQNWSCKLFWESMFYALKISSIYRLHDGNGEDVYLAFNAHDY 60

Query: 61  FVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGSTYNLAPYSSILLKAKLENELL 120
           FVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGSTYNLAPYSSILLKAKLENELL
Sbjct: 61  FVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGSTYNLAPYSSILLKAKLENELL 120

[Close]

BLAST of Carg02988 vs. NCBI nr
Match: XP_022936809.1 (isoamylase 3, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 167.2 bits (422), Expect = 8.9e-38
Identity = 79/80 (98.75%), Postives = 79/80 (98.75%), Query Frame = 0


Query: 41  YRLHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGST 100
           Y LHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGST
Sbjct: 695 YTLHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGST 754

Query: 101 YNLAPYSSILLKAKLENELL 121
           YNLAPYSSILLKAKLENELL
Sbjct: 755 YNLAPYSSILLKAKLENELL 774

[Close]

BLAST of Carg02988 vs. NCBI nr
Match: XP_022936810.1 (isoamylase 3, chloroplastic isoform X2 [Cucurbita moschata])

HSP 1 Score: 167.2 bits (422), Expect = 8.9e-38
Identity = 79/80 (98.75%), Postives = 79/80 (98.75%), Query Frame = 0


Query: 41  YRLHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGST 100
           Y LHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGST
Sbjct: 606 YTLHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGST 665

Query: 101 YNLAPYSSILLKAKLENELL 121
           YNLAPYSSILLKAKLENELL
Sbjct: 666 YNLAPYSSILLKAKLENELL 685

[Close]

BLAST of Carg02988 vs. NCBI nr
Match: XP_022975928.1 (isoamylase 3, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 164.9 bits (416), Expect = 4.4e-37
Identity = 78/80 (97.50%), Postives = 78/80 (97.50%), Query Frame = 0


Query: 41  YRLHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGST 100
           Y LHDGNGEDVYLAFNAHDYFVSVSLPSPPT RKWFRVVDTNLESPQDFVLDGIRGIGST
Sbjct: 693 YTLHDGNGEDVYLAFNAHDYFVSVSLPSPPTKRKWFRVVDTNLESPQDFVLDGIRGIGST 752

Query: 101 YNLAPYSSILLKAKLENELL 121
           YNLAPYSSILLKAKLENELL
Sbjct: 753 YNLAPYSSILLKAKLENELL 772

[Close]

BLAST of Carg02988 vs. NCBI nr
Match: XP_023535183.1 (isoamylase 3, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 164.9 bits (416), Expect = 4.4e-37
Identity = 78/80 (97.50%), Postives = 78/80 (97.50%), Query Frame = 0


Query: 41  YRLHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGST 100
           Y LHDGNGEDVYLAFNAHDYFVSVSLPSPPT RKWFRVVDTNLESPQDFVLDGIRGIGST
Sbjct: 693 YTLHDGNGEDVYLAFNAHDYFVSVSLPSPPTKRKWFRVVDTNLESPQDFVLDGIRGIGST 752

Query: 101 YNLAPYSSILLKAKLENELL 121
           YNLAPYSSILLKAKLENELL
Sbjct: 753 YNLAPYSSILLKAKLENELL 772

[Close]

BLAST of Carg02988 vs. ExPASy Swiss-Prot
Match: Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 110.2 bits (274), Expect = 1.7e-23
Identity = 46/75 (61.33%), Postives = 59/75 (78.67%), Query Frame = 0


Query: 41  YRLHDG-NGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGS 100
           + LHDG  G D+Y+AFNAHDYFV   +P PP  ++WFRV DTNLESP DFV +G+ G+  
Sbjct: 690 FTLHDGIGGRDIYVAFNAHDYFVKALIPQPPPGKQWFRVADTNLESPDDFVREGVAGVAD 749

Query: 101 TYNLAPYSSILLKAK 115
           TYN+AP+SSILL++K
Sbjct: 750 TYNVAPFSSILLQSK 764

[Close]

BLAST of Carg02988 vs. ExPASy Swiss-Prot
Match: B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 5.1e-20
Identity = 46/75 (61.33%), Postives = 55/75 (73.33%), Query Frame = 0


Query: 41  YRLHDGN-GEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGS 100
           + +HD N G D+YLAFNAHDYFV   +P PP ++ W RVVDTNLESP D V +G+   G 
Sbjct: 707 FTVHDHNSGGDIYLAFNAHDYFVDAVIPPPPHHKCWNRVVDTNLESPNDIVPEGVPFTGP 766

Query: 101 TYNLAPYSSILLKAK 115
            Y +APYSSILLKAK
Sbjct: 767 KYRIAPYSSILLKAK 781

[Close]

BLAST of Carg02988 vs. ExPASy Swiss-Prot
Match: Q8L735 (Isoamylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA2 PE=1 SV=2)

HSP 1 Score: 50.8 bits (120), Expect = 1.2e-05
Identity = 34/104 (32.69%), Postives = 49/104 (47.12%), Query Frame = 0


Query: 28  WE---SMFYALKI---------SSIYRLHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKW 87
           WE   S F AL+I         +S+   ++    D+++ FNA D+  SV LPS P   KW
Sbjct: 773 WEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKW 832

Query: 88  FRVVDTNLESPQDFVLDGIRGIGS-------TYNLAPYSSILLK 113
            R+VDT L  P  F ++G   +          Y + PYS  L +
Sbjct: 833 RRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

[Close]

BLAST of Carg02988 vs. ExPASy Swiss-Prot
Match: P10342 (Isoamylase OS=Pseudomonas amyloderamosa OX=32043 GN=iam PE=1 SV=3)

HSP 1 Score: 48.5 bits (114), Expect = 6.1e-05
Identity = 26/75 (34.67%), Postives = 40/75 (53.33%), Query Frame = 0


Query: 46  GNGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTN--LESPQDFVLDG----IRGIGS 105
           G+   +Y+A+N     V+ +LP+PP+  +W+RV DT    +    FV  G    I G G+
Sbjct: 702 GDSNSIYVAYNGWSSSVTFTLPAPPSGTQWYRVTDTCDWNDGASTFVAPGSETLIGGAGT 761

Query: 106 TYNLAPYSSILLKAK 115
           TY     S +LL +K
Sbjct: 762 TYGQCGQSLLLLISK 776

[Close]

BLAST of Carg02988 vs. ExPASy Swiss-Prot
Match: P26501 (Isoamylase OS=Pseudomonas sp. (strain SMP1) OX=72588 GN=iam PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 6.1e-05
Identity = 26/75 (34.67%), Postives = 40/75 (53.33%), Query Frame = 0


Query: 46  GNGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTN--LESPQDFVLDG----IRGIGS 105
           G+   +Y+A+N     V+ +LP+PP+  +W+RV DT    +    FV  G    I G G+
Sbjct: 702 GDSNSIYVAYNGWSSSVTFTLPAPPSGTQWYRVTDTCDWNDGASTFVAPGSETLIGGAGT 761

Query: 106 TYNLAPYSSILLKAK 115
           TY     S +LL +K
Sbjct: 762 TYGQCGQSLLLLISK 776

[Close]

BLAST of Carg02988 vs. ExPASy TrEMBL
Match: A0A6J1FES0 (isoamylase 3, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443282 PE=4 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 4.3e-38
Identity = 79/80 (98.75%), Postives = 79/80 (98.75%), Query Frame = 0


Query: 41  YRLHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGST 100
           Y LHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGST
Sbjct: 606 YTLHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGST 665

Query: 101 YNLAPYSSILLKAKLENELL 121
           YNLAPYSSILLKAKLENELL
Sbjct: 666 YNLAPYSSILLKAKLENELL 685

[Close]

BLAST of Carg02988 vs. ExPASy TrEMBL
Match: A0A6J1F9D1 (isoamylase 3, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443282 PE=4 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 4.3e-38
Identity = 79/80 (98.75%), Postives = 79/80 (98.75%), Query Frame = 0


Query: 41  YRLHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGST 100
           Y LHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGST
Sbjct: 695 YTLHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGST 754

Query: 101 YNLAPYSSILLKAKLENELL 121
           YNLAPYSSILLKAKLENELL
Sbjct: 755 YNLAPYSSILLKAKLENELL 774

[Close]

BLAST of Carg02988 vs. ExPASy TrEMBL
Match: A0A6J1II39 (isoamylase 3, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111476487 PE=4 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 2.1e-37
Identity = 78/80 (97.50%), Postives = 78/80 (97.50%), Query Frame = 0


Query: 41  YRLHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGST 100
           Y LHDGNGEDVYLAFNAHDYFVSVSLPSPPT RKWFRVVDTNLESPQDFVLDGIRGIGST
Sbjct: 606 YTLHDGNGEDVYLAFNAHDYFVSVSLPSPPTKRKWFRVVDTNLESPQDFVLDGIRGIGST 665

Query: 101 YNLAPYSSILLKAKLENELL 121
           YNLAPYSSILLKAKLENELL
Sbjct: 666 YNLAPYSSILLKAKLENELL 685

[Close]

BLAST of Carg02988 vs. ExPASy TrEMBL
Match: A0A6J1IKM9 (isoamylase 3, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476487 PE=4 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 2.1e-37
Identity = 78/80 (97.50%), Postives = 78/80 (97.50%), Query Frame = 0


Query: 41  YRLHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGST 100
           Y LHDGNGEDVYLAFNAHDYFVSVSLPSPPT RKWFRVVDTNLESPQDFVLDGIRGIGST
Sbjct: 693 YTLHDGNGEDVYLAFNAHDYFVSVSLPSPPTKRKWFRVVDTNLESPQDFVLDGIRGIGST 752

Query: 101 YNLAPYSSILLKAKLENELL 121
           YNLAPYSSILLKAKLENELL
Sbjct: 753 YNLAPYSSILLKAKLENELL 772

[Close]

BLAST of Carg02988 vs. ExPASy TrEMBL
Match: A0A0A0LE02 (Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G889910 PE=4 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.6e-32
Identity = 68/80 (85.00%), Postives = 74/80 (92.50%), Query Frame = 0


Query: 41  YRLHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGST 100
           Y LHD NGEDVYLAFNAH+YFV+VSLPSPPT RKWFRVVDTNLESP DFVLDGI G+GS+
Sbjct: 696 YTLHDDNGEDVYLAFNAHEYFVNVSLPSPPTKRKWFRVVDTNLESPHDFVLDGIPGVGSS 755

Query: 101 YNLAPYSSILLKAKLENELL 121
           YN+APYSSILLKA LEN+LL
Sbjct: 756 YNVAPYSSILLKANLENDLL 775

[Close]

BLAST of Carg02988 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 110.2 bits (274), Expect = 1.2e-24
Identity = 46/75 (61.33%), Postives = 59/75 (78.67%), Query Frame = 0


Query: 41  YRLHDG-NGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGS 100
           + LHDG  G D+Y+AFNAHDYFV   +P PP  ++WFRV DTNLESP DFV +G+ G+  
Sbjct: 690 FTLHDGIGGRDIYVAFNAHDYFVKALIPQPPPGKQWFRVADTNLESPDDFVREGVAGVAD 749

Query: 101 TYNLAPYSSILLKAK 115
           TYN+AP+SSILL++K
Sbjct: 750 TYNVAPFSSILLQSK 764

[Close]

BLAST of Carg02988 vs. TAIR 10
Match: AT1G03310.2 (debranching enzyme 1 )

HSP 1 Score: 50.8 bits (120), Expect = 8.7e-07
Identity = 34/104 (32.69%), Postives = 49/104 (47.12%), Query Frame = 0


Query: 28  WE---SMFYALKI---------SSIYRLHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKW 87
           WE   S F AL+I         +S+   ++    D+++ FNA D+  SV LPS P   KW
Sbjct: 773 WEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKW 832

Query: 88  FRVVDTNLESPQDFVLDGIRGIGS-------TYNLAPYSSILLK 113
            R+VDT L  P  F ++G   +          Y + PYS  L +
Sbjct: 833 RRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

[Close]

BLAST of Carg02988 vs. TAIR 10
Match: AT1G03310.1 (debranching enzyme 1 )

HSP 1 Score: 50.8 bits (120), Expect = 8.7e-07
Identity = 34/104 (32.69%), Postives = 49/104 (47.12%), Query Frame = 0


Query: 28  WE---SMFYALKI---------SSIYRLHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKW 87
           WE   S F AL+I         +S+   ++    D+++ FNA D+  SV LPS P   KW
Sbjct: 773 WEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKW 832

Query: 88  FRVVDTNLESPQDFVLDGIRGIGS-------TYNLAPYSSILLK 113
            R+VDT L  P  F ++G   +          Y + PYS  L +
Sbjct: 833 RRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

The following BLAST results are available for this feature:

BLAST of Carg02988 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7024561.1	7.2e-64	100.00	Isoamylase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]
XP_022936809.1	8.9e-38	98.75	isoamylase 3, chloroplastic isoform X1 [Cucurbita moschata]	[more]
XP_022936810.1	8.9e-38	98.75	isoamylase 3, chloroplastic isoform X2 [Cucurbita moschata]	[more]
XP_022975928.1	4.4e-37	97.50	isoamylase 3, chloroplastic isoform X1 [Cucurbita maxima]	[more]
XP_023535183.1	4.4e-37	97.50	isoamylase 3, chloroplastic [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg02988 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M0S5	1.7e-23	61.33	Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2	[more]
B9G434	5.1e-20	61.33	Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=...	[more]
Q8L735	1.2e-05	32.69	Isoamylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA2 PE=1 SV=2	[more]
P10342	6.1e-05	34.67	Isoamylase OS=Pseudomonas amyloderamosa OX=32043 GN=iam PE=1 SV=3	[more]
P26501	6.1e-05	34.67	Isoamylase OS=Pseudomonas sp. (strain SMP1) OX=72588 GN=iam PE=1 SV=1	[more]

BLAST of Carg02988 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FES0	4.3e-38	98.75	isoamylase 3, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1F9D1	4.3e-38	98.75	isoamylase 3, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1II39	2.1e-37	97.50	isoamylase 3, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1114764...	[more]
A0A6J1IKM9	2.1e-37	97.50	isoamylase 3, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114764...	[more]
A0A0A0LE02	1.6e-32	85.00	Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G889910 PE=4 S...	[more]

BLAST of Carg02988 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G09020.1	1.2e-24	61.33	isoamylase 3	[more]
AT1G03310.2	8.7e-07	32.69	debranching enzyme 1	[more]
AT1G03310.1	8.7e-07	32.69	debranching enzyme 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013780	Glycosyl hydrolase, all-beta	GENE3D	2.60.40.1180		coord: 19..119 e-value: 1.1E-19 score: 72.6
None	No IPR available	PANTHER	PTHR43002	GLYCOGEN DEBRANCHING ENZYME	coord: 29..113
None	No IPR available	SUPERFAMILY	51011	Glycosyl hydrolase domain	coord: 44..114

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg02988-RA	Carg02988-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005977	glycogen metabolic process
molecular_function	GO:0019156	isoamylase activity