Carg02880 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg02880
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionAuxin-responsive protein
LocationCarg_Chr09: 2207414 .. 2208432 (+)
RNA-Seq ExpressionCarg02880
SyntenyCarg02880
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGTTGCTAACAAAACTACCCATTCGATCCACTGCTAATGCTAGCTAGCTACCCTCATCCCATTGCCTTTGAATTATTTGAAAACAGGAAAGATCCTAATGGAGCTTCAACTTGAGCTAGCGCTTTGTGTATCAGATCCCACCAGAGGTTTCGACCTGAACAGGGCTGCGTGTGATGGAAAAGATGTGTTTGGCTCAGATCCGAGGAGCTGTCTGTGTGCAGAAAGTACAAGCCATGGTAGACACAAGCGTGGATATGAAGATGCTTTCTTTAAGAGCAAAGACTCGTTTAAAGAGCTGTCTTTGCTGCTGTGGAGCGGTCACCCAAACAAGGAAGACGATGACTGGAAGGACACCAACCAGAGAAGCTCTTGTGCTCTACACATGTGAGTTCCATTTCCTCAACTGATTTTTCCTCTTTTAGAAAACTTTAATCTATGCTCGTTAGATAAATATGCTGATTTTTATCCTAACAGTCGATGGTTCAATCCTCCACTCCATAATTCAAAAGAAGTATTATTACGTAGGGAGAGAATTTGAACATCGACCCATGAGAGTAGACACTGGTATTTAACTACTTGGAGTTATGTGTTGATGAACTGATATACATCAAACAAGGAGATAATATATATTTCTCTGTTTTTAAGTTGGATTTCAGTTCTTATTTTCAATTATGGGTCTGAATTTCATTTTCAAACTTAAAATTGATGATTTGTTCCATTTTTCATAGCAGAAATGCTGGAGAGGAGAACAAAGTTATGGGTTGGCCGCCCATTAAATCGTGGAGGAAAAAGTACCTTCATGAACTGCAACAGGATGGCTTTGGTTCTGATCAGATACCAAATCACTATTGGATGGAAGAGGATGAAGATGATGAAATTGTTTTTAACCCAAAATATGTGAAGGTGAAGATGGAGGGGGTGCCAATAGCAAGGAAGATTGATGTGGGGCTGTATAGCTCTTATCAGACGCTGAAAACTGCCTTGATCCACATGTTTTCCGATTCTTGCTGTGAGTGA

mRNA sequence

AAAGTTGCTAACAAAACTACCCATTCGATCCACTGCTAATGCTAGCTAGCTACCCTCATCCCATTGCCTTTGAATTATTTGAAAACAGGAAAGATCCTAATGGAGCTTCAACTTGAGCTAGCGCTTTGTGTATCAGATCCCACCAGAGGTTTCGACCTGAACAGGGCTGCGTGTGATGGAAAAGATGTGTTTGGCTCAGATCCGAGGAGCTGTCTGTGTGCAGAAAGTACAAGCCATGGTAGACACAAGCGTGGATATGAAGATGCTTTCTTTAAGAGCAAAGACTCGTTTAAAGAGCTGTCTTTGCTGCTGTGGAGCGGTCACCCAAACAAGGAAGACGATGACTGGAAGGACACCAACCAGAGAAGCTCTTGTGCTCTACACATCAGAAATGCTGGAGAGGAGAACAAAGTTATGGGTTGGCCGCCCATTAAATCGTGGAGGAAAAAGTACCTTCATGAACTGCAACAGGATGGCTTTGGTTCTGATCAGATACCAAATCACTATTGGATGGAAGAGGATGAAGATGATGAAATTGTTTTTAACCCAAAATATGTGAAGGTGAAGATGGAGGGGGTGCCAATAGCAAGGAAGATTGATGTGGGGCTGTATAGCTCTTATCAGACGCTGAAAACTGCCTTGATCCACATGTTTTCCGATTCTTGCTGTGAGTGA

Coding sequence (CDS)

ATGGAGCTTCAACTTGAGCTAGCGCTTTGTGTATCAGATCCCACCAGAGGTTTCGACCTGAACAGGGCTGCGTGTGATGGAAAAGATGTGTTTGGCTCAGATCCGAGGAGCTGTCTGTGTGCAGAAAGTACAAGCCATGGTAGACACAAGCGTGGATATGAAGATGCTTTCTTTAAGAGCAAAGACTCGTTTAAAGAGCTGTCTTTGCTGCTGTGGAGCGGTCACCCAAACAAGGAAGACGATGACTGGAAGGACACCAACCAGAGAAGCTCTTGTGCTCTACACATCAGAAATGCTGGAGAGGAGAACAAAGTTATGGGTTGGCCGCCCATTAAATCGTGGAGGAAAAAGTACCTTCATGAACTGCAACAGGATGGCTTTGGTTCTGATCAGATACCAAATCACTATTGGATGGAAGAGGATGAAGATGATGAAATTGTTTTTAACCCAAAATATGTGAAGGTGAAGATGGAGGGGGTGCCAATAGCAAGGAAGATTGATGTGGGGCTGTATAGCTCTTATCAGACGCTGAAAACTGCCTTGATCCACATGTTTTCCGATTCTTGCTGTGAGTGA

Protein sequence

MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKSKDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLHELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTALIHMFSDSCCE
Homology
BLAST of Carg02880 vs. NCBI nr
Match: KAG7024457.1 (Auxin-responsive protein IAA27 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 409.1 bits (1050), Expect = 2.1e-110
Identity = 191/191 (100.00%), Postives = 191/191 (100.00%), Query Frame = 0

Query: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60
           MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS
Sbjct: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60

Query: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH 120
           KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH
Sbjct: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH 120

Query: 121 ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA 180
           ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA
Sbjct: 121 ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA 180

Query: 181 LIHMFSDSCCE 192
           LIHMFSDSCCE
Sbjct: 181 LIHMFSDSCCE 191

BLAST of Carg02880 vs. NCBI nr
Match: XP_022937067.1 (auxin-responsive protein IAA28 isoform X1 [Cucurbita moschata])

HSP 1 Score: 402.1 bits (1032), Expect = 2.6e-108
Identity = 188/189 (99.47%), Postives = 189/189 (100.00%), Query Frame = 0

Query: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60
           MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS
Sbjct: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60

Query: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH 120
           KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH
Sbjct: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH 120

Query: 121 ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA 180
           ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTL+TA
Sbjct: 121 ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLETA 180

Query: 181 LIHMFSDSC 190
           LIHMFSDSC
Sbjct: 181 LIHMFSDSC 189

BLAST of Carg02880 vs. NCBI nr
Match: XP_022976192.1 (auxin-responsive protein IAA28-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 398.3 bits (1022), Expect = 3.8e-107
Identity = 187/189 (98.94%), Postives = 188/189 (99.47%), Query Frame = 0

Query: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60
           MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS
Sbjct: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60

Query: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH 120
           KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH
Sbjct: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH 120

Query: 121 ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA 180
           ELQQDGFGSDQIPNHYWMEEDED+EIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA
Sbjct: 121 ELQQDGFGSDQIPNHYWMEEDEDEEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA 180

Query: 181 LIHMFSDSC 190
           LI MFSDSC
Sbjct: 181 LIPMFSDSC 189

BLAST of Carg02880 vs. NCBI nr
Match: KAG6591567.1 (Auxin-responsive protein IAA29, partial [Cucurbita argyrosperma subsp. sororia] >KAG6591604.1 Auxin-responsive protein IAA29, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 397.1 bits (1019), Expect = 8.4e-107
Identity = 188/189 (99.47%), Postives = 188/189 (99.47%), Query Frame = 0

Query: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60
           MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS
Sbjct: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60

Query: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH 120
           KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHI NAGEENKVMGWPPIKSWRKKYLH
Sbjct: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHI-NAGEENKVMGWPPIKSWRKKYLH 120

Query: 121 ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA 180
           ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA
Sbjct: 121 ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA 180

Query: 181 LIHMFSDSC 190
           LIHMFSDSC
Sbjct: 181 LIHMFSDSC 188

BLAST of Carg02880 vs. NCBI nr
Match: XP_023535365.1 (auxin-responsive protein IAA28 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 396.0 bits (1016), Expect = 1.9e-106
Identity = 187/189 (98.94%), Postives = 187/189 (98.94%), Query Frame = 0

Query: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60
           MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCL AESTSHGRHKRGYEDAFFKS
Sbjct: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLRAESTSHGRHKRGYEDAFFKS 60

Query: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH 120
           KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNA EENKVMGWPPIKSWRKKYLH
Sbjct: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAEEENKVMGWPPIKSWRKKYLH 120

Query: 121 ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA 180
           ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA
Sbjct: 121 ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA 180

Query: 181 LIHMFSDSC 190
           LIHMFSDSC
Sbjct: 181 LIHMFSDSC 189

BLAST of Carg02880 vs. ExPASy Swiss-Prot
Match: Q93WC4 (Auxin-responsive protein IAA29 OS=Arabidopsis thaliana OX=3702 GN=IAA29 PE=1 SV=2)

HSP 1 Score: 63.2 bits (152), Expect = 3.8e-09
Identity = 61/214 (28.50%), Postives = 94/214 (43.93%), Query Frame = 0

Query: 1   MELQLELALCVSDPTR---GFDLNR-AACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDA 60
           MEL L L+L     ++    FDLN+  A +G         SCL       G  K  +E  
Sbjct: 1   MELDLGLSLSPHKSSKLGFNFDLNKHCAIEG-------AASCL-------GTEKLRFEAT 60

Query: 61  F----FKSKDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIK 120
           F     +      +  L   +G PN+ED+D       S  ++   +  E ++V+GWPP+K
Sbjct: 61  FGLGNVEENCYMPKQRLFALNGQPNEEDED----PLESESSIVYDDEEENSEVVGWPPVK 120

Query: 121 SWRKKYLHELQQDGFGSDQIPNHY-WMEEDEDDEIVFNP------------------KYV 180
           +   KY     +        P H+        +  + NP                   YV
Sbjct: 121 TCMIKYGSYHHRHIRNHHHCPYHHRGRRITAMNNNISNPTTATVGSSSSSSISSRSSMYV 180

Query: 181 KVKMEGVPIARKIDVGLYSSYQTLKTALIHMFSD 188
           KVKM+GV IARK+D+ L++SY++L  +LI MF++
Sbjct: 181 KVKMDGVAIARKVDIKLFNSYESLTNSLITMFTE 196

BLAST of Carg02880 vs. ExPASy Swiss-Prot
Match: P93830 (Auxin-responsive protein IAA17 OS=Arabidopsis thaliana OX=3702 GN=IAA17 PE=1 SV=2)

HSP 1 Score: 60.8 bits (146), Expect = 1.9e-08
Identity = 32/102 (31.37%), Postives = 50/102 (49.02%), Query Frame = 0

Query: 85  DTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLHELQQDGFGSDQIPNHYWMEEDEDD 144
           D+ ++S+C         + +V+GWPP++S+RK  +   Q+   G +              
Sbjct: 63  DSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA------------- 122

Query: 145 EIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTALIHMFS 187
                  +VKV M+G P  RKID+ +Y SY  L  AL +MFS
Sbjct: 123 -----AAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFS 146

BLAST of Carg02880 vs. ExPASy Swiss-Prot
Match: A2WTQ5 (Auxin-responsive protein IAA5 OS=Oryza sativa subsp. indica OX=39946 GN=IAA5 PE=2 SV=2)

HSP 1 Score: 60.1 bits (144), Expect = 3.2e-08
Identity = 32/83 (38.55%), Postives = 44/83 (53.01%), Query Frame = 0

Query: 104 KVMGWPPIKSWRKKYLHELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIA 163
           +V+GWPP++++RK  L      G G D+                  P YVKV M+G P  
Sbjct: 118 QVVGWPPVRNYRKNTLAASASKGKGEDK------------GTAEGGPLYVKVSMDGAPYL 177

Query: 164 RKIDVGLYSSYQTLKTALIHMFS 187
           RK+D+ +YSSY+ L  AL  MFS
Sbjct: 178 RKVDLKMYSSYEDLSMALEKMFS 188

BLAST of Carg02880 vs. ExPASy Swiss-Prot
Match: Q0JKG7 (Auxin-responsive protein IAA5 OS=Oryza sativa subsp. japonica OX=39947 GN=IAA5 PE=2 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 3.2e-08
Identity = 32/83 (38.55%), Postives = 44/83 (53.01%), Query Frame = 0

Query: 104 KVMGWPPIKSWRKKYLHELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIA 163
           +V+GWPP++++RK  L      G G D+                  P YVKV M+G P  
Sbjct: 117 QVVGWPPVRNYRKNTLAASASKGKGEDK------------GTAEGGPLYVKVSMDGAPYL 176

Query: 164 RKIDVGLYSSYQTLKTALIHMFS 187
           RK+D+ +YSSY+ L  AL  MFS
Sbjct: 177 RKVDLKMYSSYEDLSMALEKMFS 187

BLAST of Carg02880 vs. ExPASy Swiss-Prot
Match: Q5VRR0 (Auxin-responsive protein IAA20 OS=Oryza sativa subsp. japonica OX=39947 GN=IAA20 PE=2 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 2.1e-07
Identity = 55/187 (29.41%), Postives = 74/187 (39.57%), Query Frame = 0

Query: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAF--F 60
           MEL+L L L +  P+       AA    D+  S P SC           KRG+E+A   F
Sbjct: 1   MELELGLRLALPSPSPSPATATAAGSELDLLNSAPGSC----------RKRGFEEALGGF 60

Query: 61  KSKDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKY 120
           K+ D   +      +G     D D +  N+R              K++GWPP+K      
Sbjct: 61  KTDDDNDD-----GNGRGGDGDSDGEMGNKR-------------RKLVGWPPVKC----- 120

Query: 121 LHELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLK 180
           LH  +  G G                       YVKVKMEG+ I RK+D+ +  SY  L 
Sbjct: 121 LHRRRDGGCGGG---------------------YVKVKMEGLAIGRKLDLSILGSYAELL 133

Query: 181 TALIHMF 186
             L  MF
Sbjct: 181 DTLHLMF 133

BLAST of Carg02880 vs. ExPASy TrEMBL
Match: A0A6J1FA34 (Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111443480 PE=3 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 1.3e-108
Identity = 188/189 (99.47%), Postives = 189/189 (100.00%), Query Frame = 0

Query: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60
           MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS
Sbjct: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60

Query: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH 120
           KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH
Sbjct: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH 120

Query: 121 ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA 180
           ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTL+TA
Sbjct: 121 ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLETA 180

Query: 181 LIHMFSDSC 190
           LIHMFSDSC
Sbjct: 181 LIHMFSDSC 189

BLAST of Carg02880 vs. ExPASy TrEMBL
Match: A0A6J1IF49 (Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111476655 PE=3 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 1.8e-107
Identity = 187/189 (98.94%), Postives = 188/189 (99.47%), Query Frame = 0

Query: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60
           MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS
Sbjct: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60

Query: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH 120
           KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH
Sbjct: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH 120

Query: 121 ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA 180
           ELQQDGFGSDQIPNHYWMEEDED+EIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA
Sbjct: 121 ELQQDGFGSDQIPNHYWMEEDEDEEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA 180

Query: 181 LIHMFSDSC 190
           LI MFSDSC
Sbjct: 181 LIPMFSDSC 189

BLAST of Carg02880 vs. ExPASy TrEMBL
Match: A0A6J1FF02 (Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111443480 PE=3 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.2e-106
Identity = 187/189 (98.94%), Postives = 188/189 (99.47%), Query Frame = 0

Query: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60
           MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS
Sbjct: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60

Query: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH 120
           KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHI NAGEENKVMGWPPIKSWRKKYLH
Sbjct: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHI-NAGEENKVMGWPPIKSWRKKYLH 120

Query: 121 ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA 180
           ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTL+TA
Sbjct: 121 ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLETA 180

Query: 181 LIHMFSDSC 190
           LIHMFSDSC
Sbjct: 181 LIHMFSDSC 188

BLAST of Carg02880 vs. ExPASy TrEMBL
Match: A0A6J1ILE5 (Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111476655 PE=3 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 1.7e-105
Identity = 186/189 (98.41%), Postives = 187/189 (98.94%), Query Frame = 0

Query: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60
           MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS
Sbjct: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60

Query: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH 120
           KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHI NAGEENKVMGWPPIKSWRKKYLH
Sbjct: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHI-NAGEENKVMGWPPIKSWRKKYLH 120

Query: 121 ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA 180
           ELQQDGFGSDQIPNHYWMEEDED+EIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA
Sbjct: 121 ELQQDGFGSDQIPNHYWMEEDEDEEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA 180

Query: 181 LIHMFSDSC 190
           LI MFSDSC
Sbjct: 181 LIPMFSDSC 188

BLAST of Carg02880 vs. ExPASy TrEMBL
Match: A0A6J1FJ11 (Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111445918 PE=3 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 6.3e-84
Identity = 156/189 (82.54%), Postives = 169/189 (89.42%), Query Frame = 0

Query: 1   MELQLELALCVSDPTRGFDLNRAACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDAFFKS 60
           MELQLELAL V D TRGFDLNR ACD +DVFGSDPRSC+C +STSHGRHKRG+EDAFFKS
Sbjct: 68  MELQLELALSVPDRTRGFDLNRNACD-RDVFGSDPRSCVCEQSTSHGRHKRGFEDAFFKS 127

Query: 61  KDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLH 120
           KDS K+LSLLLWS   NKEDDD KDTN RSSCA+HI N GEENKV+GWPPIKSWRKK+LH
Sbjct: 128 KDSSKDLSLLLWSDRSNKEDDDRKDTNHRSSCAIHI-NVGEENKVVGWPPIKSWRKKHLH 187

Query: 121 ELQQDGFGSDQIPNHYWMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTA 180
           +LQQD  GSDQ  NHYWME++EDD IVFNPKYVKVKMEGV IARKIDVGLYSSYQTLKTA
Sbjct: 188 QLQQDRLGSDQTNNHYWMEDNEDDGIVFNPKYVKVKMEGVAIARKIDVGLYSSYQTLKTA 247

Query: 181 LIHMFSDSC 190
           LI+MFS+SC
Sbjct: 248 LINMFSNSC 254

BLAST of Carg02880 vs. TAIR 10
Match: AT4G32280.1 (indole-3-acetic acid inducible 29 )

HSP 1 Score: 63.2 bits (152), Expect = 2.7e-10
Identity = 61/214 (28.50%), Postives = 94/214 (43.93%), Query Frame = 0

Query: 1   MELQLELALCVSDPTR---GFDLNR-AACDGKDVFGSDPRSCLCAESTSHGRHKRGYEDA 60
           MEL L L+L     ++    FDLN+  A +G         SCL       G  K  +E  
Sbjct: 1   MELDLGLSLSPHKSSKLGFNFDLNKHCAIEG-------AASCL-------GTEKLRFEAT 60

Query: 61  F----FKSKDSFKELSLLLWSGHPNKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIK 120
           F     +      +  L   +G PN+ED+D       S  ++   +  E ++V+GWPP+K
Sbjct: 61  FGLGNVEENCYMPKQRLFALNGQPNEEDED----PLESESSIVYDDEEENSEVVGWPPVK 120

Query: 121 SWRKKYLHELQQDGFGSDQIPNHY-WMEEDEDDEIVFNP------------------KYV 180
           +   KY     +        P H+        +  + NP                   YV
Sbjct: 121 TCMIKYGSYHHRHIRNHHHCPYHHRGRRITAMNNNISNPTTATVGSSSSSSISSRSSMYV 180

Query: 181 KVKMEGVPIARKIDVGLYSSYQTLKTALIHMFSD 188
           KVKM+GV IARK+D+ L++SY++L  +LI MF++
Sbjct: 181 KVKMDGVAIARKVDIKLFNSYESLTNSLITMFTE 196

BLAST of Carg02880 vs. TAIR 10
Match: AT1G04250.1 (AUX/IAA transcriptional regulator family protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.3e-09
Identity = 32/102 (31.37%), Postives = 50/102 (49.02%), Query Frame = 0

Query: 85  DTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLHELQQDGFGSDQIPNHYWMEEDEDD 144
           D+ ++S+C         + +V+GWPP++S+RK  +   Q+   G +              
Sbjct: 63  DSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA------------- 122

Query: 145 EIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTALIHMFS 187
                  +VKV M+G P  RKID+ +Y SY  L  AL +MFS
Sbjct: 123 -----AAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFS 146

BLAST of Carg02880 vs. TAIR 10
Match: AT4G29080.1 (phytochrome-associated protein 2 )

HSP 1 Score: 58.9 bits (141), Expect = 5.1e-09
Identity = 36/87 (41.38%), Postives = 47/87 (54.02%), Query Frame = 0

Query: 104 KVMGWPPIKSWRKKYLHELQQDGFGSDQIPNHYWMEEDEDDEIVFNPK----YVKVKMEG 163
           +V+GWPPI+S+RK  +   Q    G++         E E+ E    P+    YVKV MEG
Sbjct: 143 QVVGWPPIRSFRKNSMASSQSQKPGNN--------SETEEAEAKSGPEQPCLYVKVSMEG 202

Query: 164 VPIARKIDVGLYSSYQTLKTALIHMFS 187
            P  RKID+  Y SY  L +AL  MFS
Sbjct: 203 APYLRKIDLKTYKSYLELSSALEKMFS 221

BLAST of Carg02880 vs. TAIR 10
Match: AT5G25890.1 (indole-3-acetic acid inducible 28 )

HSP 1 Score: 56.2 bits (134), Expect = 3.3e-08
Identity = 36/110 (32.73%), Postives = 59/110 (53.64%), Query Frame = 0

Query: 77  NKEDDDWKDTNQRSSCALHIRNAGEENKVMGWPPIKSWRKKYLHELQQDGFGSDQIPNHY 136
           +K+    K+T+  S+      N  E   V+GWPP++S R+    +L+++           
Sbjct: 27  SKQKSSTKETSFLSN------NRVEVAPVVGWPPVRSSRRNLTAQLKEE----------- 86

Query: 137 WMEEDEDDEIVFNPKYVKVKMEGVPIARKIDVGLYSSYQTLKTALIHMFS 187
            M++ E DE      YVK+ MEGVPI RK+++  Y++YQ L  A+  +FS
Sbjct: 87  -MKKKESDE--EKELYVKINMEGVPIGRKVNLSAYNNYQQLSHAVDQLFS 116

BLAST of Carg02880 vs. TAIR 10
Match: AT3G16500.1 (phytochrome-associated protein 1 )

HSP 1 Score: 53.9 bits (128), Expect = 1.6e-07
Identity = 28/84 (33.33%), Postives = 47/84 (55.95%), Query Frame = 0

Query: 105 VMGWPPIKSWRKKYLHELQQDGFGSDQIPNHYWMEEDEDDEIVFNPK---YVKVKMEGVP 164
           V+GWPP++S+RK  L        G++        + D+ ++ V   K   +VK+ M+GVP
Sbjct: 104 VVGWPPVRSFRKN-LASTSSSKLGNESSHGGQINKSDDGEKQVETKKEGMFVKINMDGVP 163

Query: 165 IARKIDVGLYSSYQTLKTALIHMF 186
           I RK+D+  Y+SY+ L   +  +F
Sbjct: 164 IGRKVDLNAYNSYEQLSFVVDKLF 186

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7024457.12.1e-110100.00Auxin-responsive protein IAA27 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022937067.12.6e-10899.47auxin-responsive protein IAA28 isoform X1 [Cucurbita moschata][more]
XP_022976192.13.8e-10798.94auxin-responsive protein IAA28-like isoform X1 [Cucurbita maxima][more]
KAG6591567.18.4e-10799.47Auxin-responsive protein IAA29, partial [Cucurbita argyrosperma subsp. sororia] ... [more]
XP_023535365.11.9e-10698.94auxin-responsive protein IAA28 isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q93WC43.8e-0928.50Auxin-responsive protein IAA29 OS=Arabidopsis thaliana OX=3702 GN=IAA29 PE=1 SV=... [more]
P938301.9e-0831.37Auxin-responsive protein IAA17 OS=Arabidopsis thaliana OX=3702 GN=IAA17 PE=1 SV=... [more]
A2WTQ53.2e-0838.55Auxin-responsive protein IAA5 OS=Oryza sativa subsp. indica OX=39946 GN=IAA5 PE=... [more]
Q0JKG73.2e-0838.55Auxin-responsive protein IAA5 OS=Oryza sativa subsp. japonica OX=39947 GN=IAA5 P... [more]
Q5VRR02.1e-0729.41Auxin-responsive protein IAA20 OS=Oryza sativa subsp. japonica OX=39947 GN=IAA20... [more]
Match NameE-valueIdentityDescription
A0A6J1FA341.3e-10899.47Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111443480 PE=3 SV=1[more]
A0A6J1IF491.8e-10798.94Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111476655 PE=3 SV=1[more]
A0A6J1FF021.2e-10698.94Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111443480 PE=3 SV=1[more]
A0A6J1ILE51.7e-10598.41Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111476655 PE=3 SV=1[more]
A0A6J1FJ116.3e-8482.54Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111445918 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G32280.12.7e-1028.50indole-3-acetic acid inducible 29 [more]
AT1G04250.11.3e-0931.37AUX/IAA transcriptional regulator family protein [more]
AT4G29080.15.1e-0941.38phytochrome-associated protein 2 [more]
AT5G25890.13.3e-0832.73indole-3-acetic acid inducible 28 [more]
AT3G16500.11.6e-0733.33phytochrome-associated protein 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.10.20.90coord: 146..191
e-value: 1.5E-10
score: 42.9
NoneNo IPR availablePANTHERPTHR31734:SF44AUXIN-RESPONSIVE PROTEIN IAA29coord: 1..188
IPR033389AUX/IAA domainPFAMPF02309AUX_IAAcoord: 45..187
e-value: 2.3E-22
score: 80.0
IPR003311AUX/IAA proteinPANTHERPTHR31734AUXIN-RESPONSIVE PROTEIN IAA17coord: 1..188
IPR000270PB1 domainPROSITEPS51745PB1coord: 150..191
score: 14.996021

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg02880-RACarg02880-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009734 auxin-activated signaling pathway
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding