Carg02867 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg02867
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionER membrane protein complex subunit 7 homolog
LocationCarg_Chr09: 2134431 .. 2137700 (-)
RNA-Seq ExpressionCarg02867
SyntenyCarg02867
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TAGTAAAAACTTAAAAAGTTAAATTAATTTATCCCTCAAAAATAAAATCAGATGGGATCGAGAGGGAGAGGGATGGAGTAAAATCAGGTATCCGTTCTTTCTTTCTACAACACTCGGCAAAGAACAAGAAGAACCGCGAGATCTTCCCTGTTCCGAACCTCCCGCCATTGCAGAATCTTCAATAATGCGATCTCAAGTTTTCATTCTCTTGCTTTCACTCCAATTATTCTTCTCTCTGTTATCTCCGTCGCATGCGATTTCCCCTGGGTAGGTCTCAGTCCTTGTCTTTAATTCACCGTGACTTTGTGCTCGGCTCGTCAGGAACATGAATTGGTGAAGATTGAAACTAATGATCGACGATTCCGTGGAACTAGAATGCGTCATTATTACTTTCTATTGTTTTTTAATCTCGTTGTTGCATTTCGCAGGTCCGGCGATGGATACACTATCTATGGTCGGGTGAAAATTTCAAGTACGTTGCTTTTTTTTCTTATTCTACAAGTTGTGAATGTATTTTGATATTCAGCTTCGAGGTAGTTGTAGATTTGTTACTTCTTTTTGTAACTTAAAGAAAGTTATTCGTTGGAAAACGAGAATTACAGAAGCGAAGTGAAGTGAAGATATCTGAACCTAATATGGAGGAGATTAAAAAGATTCTTCCGTTGGTATTGATAATTACCAGAAGTACTTCTTAGTTTGGCAATGAAAGAAATATTATCTTACGACTGACCAAAATTCTACAAGTTTTGAATAATTACCAGAATCTTTTTCCTAATGAGCTAAATTTAAATTACTCTGAATCTTTCCTGCTATAGATTACAAAAGTCCTTACTGCTAGGGTCTCCTGGAAGGTTAAGGTATAAGTTGTGAGCCAAGAATTGCAGCATGACCATAGGACTAAGCAATGTGGATCCTTTATCCTTAATATAAATTATTTCCCTCCACCATGTACTCGAGGTCTCCACATGAAAATAGTGATGCTGCTTTGATAATTCTGGAATATATTATCTGCATCTAGGTGTCGTGTTAGCATAGATTTGAAACGAGGAAAAAAGATTTGCAGACCCCTACCTTACTATTGTAGGTCCTCATAAAGCTCTCCTTTTGGAGGAGAGGCAACACAACTATGTTTCTTCACCCCTTCACATGAGGCATGTACGCACCTACTTTTTACTTAGCATTTTCCTCTTACTTTTGTCCATATTGAATCCCTAGGGGTGACTAGTATGTTAGAAAAATCTATCCGCTTTACAGTTCTGCATTGCGAAGATGAAAATGTTCAAAATGAATGTTATATTTGCTTGAGATTCTATGTTTAGATGATTGTCGTCGTACTATGAACTAATGCATACTGTTGAATTTTTGGCAGGGAACGCACTTAAAGAATATGGACTTCCTGGAAAAATATCAAACATTAAAGTCATACTTAATGGTGGACAAAGAGTTACCTTCCTGAGGCCCGATGGATACTTTTCATTGTATCCTCTTTATGTTTTTAGTTAATTTAAAATGCTTAAAGTTAATGAGTTTTATTACTTTTAGGCTTGTTTCCGCACAGGATTTCATTTTCTGCTTTGCTCTTGTTGGGACTCGAATGATCTGCTCGGATCATTTATACAATCCTCAACTGGATGTAGCATTTGATTTTATTTTGTTCTTTGCTTCCTAGTGGTTAGTGTTATTATTATTTTGGATAGTTAAGCTAAAAAATTTGGCTTTCCAATTTTCTGAGGTTTCTGTAATTCCTTAACTTCAGTTCTTAAAGCTATAATGTGCCAGCAGGAACCCATCTGCTTGAAGTGGCTGCATTGGGCTATTTCTTTTCTCCCGTGGGTTTTCTTTATCATCCATCGGGTATCAGTTTTTCTACAAATCTGGTTTCATGTGACTTTTTTTTTTTTCCCTTTCTATTTATTCCTTAGGTTCGAGTTGATGTGAGTGCCAGAAACCCAGGTAAGGTTCAGGCAGCCCTAACAGAGAACAGGCGGGCATTGAGTGAGCTTGTTTTAGAGCCTTTAAGGGATGAAGAGTATTATGAGGTTTGGTTTAACATAAGCTTCTCTTTTTTTTTTTTTTTTCCTTTTTAATCCACTTTTTAAGGACTATAAGTTATTGAATTTTATCTTCGATCTCACATTATTACTCAATCTCTTTGTATTATAGAGTCGGGAACCGTTCTCCATAATGTCTGTTGTGAAAAGTCCTATGGGTCTGATGGTGGGATTCATGCTAATTGTTGTATTCCTGATGCCCAAGTTAATGGAGAACATAGGTACGTACTCATTTGCTCATTAGACAGTCGTCCCTGTTTCACATCCTATAATGGCAAATTCTTGTGCTTTTAATAGTTCTTTCTTCCTTTATATTATTATGAATTTTTTTGTGTTCATTTGGTCCCTAAATTTTCAAAAGTGTTTAATAGTCAAAACTTTCATTTAATGTCGGTAGGAACCTAAGTTATCAATTTTGTATCGAGTAGATTCATGGATTTCAAAAAATATTAAATAGGTCAATTACCTATTAAGCACACAATTTTAAATTTAAGGATCAATTGGACACAAAATTGAAGTTGAAGGATATCTTTTTAGAGTTAAGGATCTTGGTGGACACAAAATTGAAATTTCATGGATTTATTAAATACTTTTGAAAATTTAGATACCAATAGATACAGACTTGAAAGTTTCGGGACTAATCTCGTCATTTAACCTATTATCGTGTTCCTATTCCATCTTGAGAATATATAGGGTAAGCTGTATTATTTTTATATACAAAGGGTTCTATGTTAATTCTCTCTTTGAAATTTCTTTGGCAATGTGTTGATGAAAAATTCTTCCAACGTTTCCACTTTGCACGAGACTTGGCAATGCCATTTCAATTTTATTACTTTAATGGATGAGATTTTCTCATGCCAAATCTGTTGGTGGAAGTTATGTTGCTCTAATTGCTTGCCCTCTCGATGAAATTGCAACTTCATTCGATTCATCATAAGAAATCAAATGGGGAAAGTTCATATTATTGGTTCCACTCCCCAATCGATTAAACTTCCAAATAGAAATGTCTTGATCGAATTCAATTCTTTCATAAATGAATGATTGTCTTTCTGCCCCCCAAACCCCCCAAAATAGAGGATGATTCTGTCTCTAACTGCGTGATTTTGCGATATTCTGATGATCAGATCCTGAAGAAATGAGGAGAGCACAAGAAGAAATGAGGAACCAAGGCGTCCCAACCTTATCAAGCTTGCTACCTGGGGCTGGCCAGAGCAGCTAA

mRNA sequence

TAGTAAAAACTTAAAAAGTTAAATTAATTTATCCCTCAAAAATAAAATCAGATGGGATCGAGAGGGAGAGGGATGGAGTAAAATCAGGTATCCGTTCTTTCTTTCTACAACACTCGGCAAAGAACAAGAAGAACCGCGAGATCTTCCCTGTTCCGAACCTCCCGCCATTGCAGAATCTTCAATAATGCGATCTCAAGTTTTCATTCTCTTGCTTTCACTCCAATTATTCTTCTCTCTGTTATCTCCGTCGCATGCGATTTCCCCTGGGTCCGGCGATGGATACACTATCTATGGTCGGGTGAAAATTTCAAGGAACGCACTTAAAGAATATGGACTTCCTGGAAAAATATCAAACATTAAAGTCATACTTAATGGTGGACAAAGAGTTACCTTCCTGAGGCCCGATGGATACTTTTCATTCTATAATGTGCCAGCAGGAACCCATCTGCTTGAAGTGGCTGCATTGGGCTATTTCTTTTCTCCCGTGGGTTTTCTTTATCATCCATCGGGTATCAGTTTTTCTACAAATCTGGTTCGAGTTGATGTGAGTGCCAGAAACCCAGGTAAGGTTCAGGCAGCCCTAACAGAGAACAGGCGGGCATTGAGTGAGCTTGTTTTAGAGCCTTTAAGGGATGAAGAGTATTATGAGAGTCGGGAACCGTTCTCCATAATGTCTGTTGTGAAAAGTCCTATGGGTCTGATGGTGGGATTCATGCTAATTGTTGTATTCCTGATGCCCAAGTTAATGGAGAACATAGATCCTGAAGAAATGAGGAGAGCACAAGAAGAAATGAGGAACCAAGGCGTCCCAACCTTATCAAGCTTGCTACCTGGGGCTGGCCAGAGCAGCTAA

Coding sequence (CDS)

ATGCGATCTCAAGTTTTCATTCTCTTGCTTTCACTCCAATTATTCTTCTCTCTGTTATCTCCGTCGCATGCGATTTCCCCTGGGTCCGGCGATGGATACACTATCTATGGTCGGGTGAAAATTTCAAGGAACGCACTTAAAGAATATGGACTTCCTGGAAAAATATCAAACATTAAAGTCATACTTAATGGTGGACAAAGAGTTACCTTCCTGAGGCCCGATGGATACTTTTCATTCTATAATGTGCCAGCAGGAACCCATCTGCTTGAAGTGGCTGCATTGGGCTATTTCTTTTCTCCCGTGGGTTTTCTTTATCATCCATCGGGTATCAGTTTTTCTACAAATCTGGTTCGAGTTGATGTGAGTGCCAGAAACCCAGGTAAGGTTCAGGCAGCCCTAACAGAGAACAGGCGGGCATTGAGTGAGCTTGTTTTAGAGCCTTTAAGGGATGAAGAGTATTATGAGAGTCGGGAACCGTTCTCCATAATGTCTGTTGTGAAAAGTCCTATGGGTCTGATGGTGGGATTCATGCTAATTGTTGTATTCCTGATGCCCAAGTTAATGGAGAACATAGATCCTGAAGAAATGAGGAGAGCACAAGAAGAAATGAGGAACCAAGGCGTCCCAACCTTATCAAGCTTGCTACCTGGGGCTGGCCAGAGCAGCTAA

Protein sequence

MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKVILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSPVGFLYHPSGISFSTNLVRVDVSARNPGKVQAALTENRRALSELVLEPLRDEEYYESREPFSIMSVVKSPMGLMVGFMLIVVFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS
Homology
BLAST of Carg02867 vs. NCBI nr
Match: KAG7024445.1 (ER membrane protein complex subunit 7-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 431.4 bits (1108), Expect = 4.7e-117
Identity = 222/222 (100.00%), Postives = 222/222 (100.00%), Query Frame = 0

Query: 1   MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV 60
           MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV
Sbjct: 1   MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV 60

Query: 61  ILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSPVGFLYHPSGISFSTNLVRVD 120
           ILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSPVGFLYHPSGISFSTNLVRVD
Sbjct: 61  ILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSPVGFLYHPSGISFSTNLVRVD 120

Query: 121 VSARNPGKVQAALTENRRALSELVLEPLRDEEYYESREPFSIMSVVKSPMGLMVGFMLIV 180
           VSARNPGKVQAALTENRRALSELVLEPLRDEEYYESREPFSIMSVVKSPMGLMVGFMLIV
Sbjct: 121 VSARNPGKVQAALTENRRALSELVLEPLRDEEYYESREPFSIMSVVKSPMGLMVGFMLIV 180

Query: 181 VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS 223
           VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS
Sbjct: 181 VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS 222

BLAST of Carg02867 vs. NCBI nr
Match: XP_022976199.1 (ER membrane protein complex subunit 7 homolog [Cucurbita maxima])

HSP 1 Score: 387.1 bits (993), Expect = 1.0e-103
Identity = 205/222 (92.34%), Postives = 206/222 (92.79%), Query Frame = 0

Query: 1   MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV 60
           MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV
Sbjct: 1   MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV 60

Query: 61  ILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSPVGFLYHPSGISFSTNLVRVD 120
           ILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSP                VRVD
Sbjct: 61  ILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSP----------------VRVD 120

Query: 121 VSARNPGKVQAALTENRRALSELVLEPLRDEEYYESREPFSIMSVVKSPMGLMVGFMLIV 180
           VSARNPGKVQAALTENRRALSELVLEPLRDEEYYE+REPFSIMSVVKSPMGLMVGFMLIV
Sbjct: 121 VSARNPGKVQAALTENRRALSELVLEPLRDEEYYETREPFSIMSVVKSPMGLMVGFMLIV 180

Query: 181 VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS 223
           VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS
Sbjct: 181 VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS 206

BLAST of Carg02867 vs. NCBI nr
Match: KAG6591557.1 (ER membrane protein complex subunit 7-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 386.0 bits (990), Expect = 2.3e-103
Identity = 205/222 (92.34%), Postives = 205/222 (92.34%), Query Frame = 0

Query: 1   MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV 60
           MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV
Sbjct: 387 MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV 446

Query: 61  ILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSPVGFLYHPSGISFSTNLVRVD 120
           ILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSP                VRVD
Sbjct: 447 ILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSP----------------VRVD 506

Query: 121 VSARNPGKVQAALTENRRALSELVLEPLRDEEYYESREPFSIMSVVKSPMGLMVGFMLIV 180
           VSARNPGKVQAALTENRRALSELVLEPLRDEEYYE REPFSIMSVVKSPMGLMVGFMLIV
Sbjct: 507 VSARNPGKVQAALTENRRALSELVLEPLRDEEYYEIREPFSIMSVVKSPMGLMVGFMLIV 566

Query: 181 VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS 223
           VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS
Sbjct: 567 VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS 592

BLAST of Carg02867 vs. NCBI nr
Match: XP_022935856.1 (ER membrane protein complex subunit 7 homolog [Cucurbita moschata])

HSP 1 Score: 384.0 bits (985), Expect = 8.6e-103
Identity = 204/222 (91.89%), Postives = 205/222 (92.34%), Query Frame = 0

Query: 1   MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV 60
           MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV
Sbjct: 1   MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV 60

Query: 61  ILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSPVGFLYHPSGISFSTNLVRVD 120
           ILNGGQRVTFLRPDGYFSFY+VPAGTHLLEVAALGYFFSP                VRVD
Sbjct: 61  ILNGGQRVTFLRPDGYFSFYDVPAGTHLLEVAALGYFFSP----------------VRVD 120

Query: 121 VSARNPGKVQAALTENRRALSELVLEPLRDEEYYESREPFSIMSVVKSPMGLMVGFMLIV 180
           VSARNPGKVQAALTENRRALSELVLEPLRDEEYYE REPFSIMSVVKSPMGLMVGFMLIV
Sbjct: 121 VSARNPGKVQAALTENRRALSELVLEPLRDEEYYEIREPFSIMSVVKSPMGLMVGFMLIV 180

Query: 181 VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS 223
           VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS
Sbjct: 181 VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS 206

BLAST of Carg02867 vs. NCBI nr
Match: XP_023535034.1 (ER membrane protein complex subunit 7 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 383.6 bits (984), Expect = 1.1e-102
Identity = 204/222 (91.89%), Postives = 204/222 (91.89%), Query Frame = 0

Query: 1   MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV 60
           MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV
Sbjct: 1   MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV 60

Query: 61  ILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSPVGFLYHPSGISFSTNLVRVD 120
           ILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGY FSP                VRVD
Sbjct: 61  ILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYLFSP----------------VRVD 120

Query: 121 VSARNPGKVQAALTENRRALSELVLEPLRDEEYYESREPFSIMSVVKSPMGLMVGFMLIV 180
           VSARNPGKVQAALTENRRALSELVLEPLRDEEYYE REPFSIMSVVKSPMGLMVGFMLIV
Sbjct: 121 VSARNPGKVQAALTENRRALSELVLEPLRDEEYYEIREPFSIMSVVKSPMGLMVGFMLIV 180

Query: 181 VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS 223
           VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS
Sbjct: 181 VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS 206

BLAST of Carg02867 vs. ExPASy Swiss-Prot
Match: Q8VY97 (ER membrane protein complex subunit 7 homolog OS=Arabidopsis thaliana OX=3702 GN=At4g32130 PE=2 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 5.5e-68
Identity = 137/210 (65.24%), Postives = 162/210 (77.14%), Query Frame = 0

Query: 9   LLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKVILNGGQRV 68
           L++  LFF   + +  IS GS D YTI GRV++  + +   G   K SNIKVILNGGQ V
Sbjct: 10  LIAFSLFFFFFASTLPISSGSEDSYTITGRVRVPASTV--IGHAAKFSNIKVILNGGQHV 69

Query: 69  TFLRPDGYFSFYNVPAGTHLLEVAALGYFFSPVGFLYHPSGISFSTNLVRVDVSARNPGK 128
           TFLRPDGYF+F+ VPAGTHL+EV ALGYFFSP                VRVDVSAR+ GK
Sbjct: 70  TFLRPDGYFTFHKVPAGTHLIEVYALGYFFSP----------------VRVDVSARHRGK 129

Query: 129 VQAALTENRRALSELVLEPLRDEEYYESREPFSIMSVVKSPMGLMVGFMLIVVFLMPKLM 188
           VQA LTE RR+L+ELVLEPLR E+YYE REPFS+MS+VKSPMGLMVGFM++VVFLMPKLM
Sbjct: 130 VQATLTETRRSLTELVLEPLRAEQYYEMREPFSVMSIVKSPMGLMVGFMVVVVFLMPKLM 189

Query: 189 ENIDPEEMRRAQEEMRNQGVPTLSSLLPGA 219
           ENIDPEEM+ AQE+MR+QGVP+L+SLLP +
Sbjct: 190 ENIDPEEMKSAQEQMRSQGVPSLTSLLPAS 201

BLAST of Carg02867 vs. ExPASy Swiss-Prot
Match: Q5TYV0 (ER membrane protein complex subunit 7 OS=Danio rerio OX=7955 GN=emc7 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.8e-11
Identity = 52/189 (27.51%), Postives = 96/189 (50.79%), Query Frame = 0

Query: 24  AISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKVILNGGQRVTFLRPDGYFSFYNVP 83
           A S  +GD + I GR  +     +++     IS  +V++ G + V FL+ DG F+  +VP
Sbjct: 33  AASQSNGDRFKIEGRAIVPGVKTQDW-----ISTARVLVEGEEYVGFLKTDGSFAVNDVP 92

Query: 84  AGTHLLEVAALGYFFSPVGFLYHPSGISFSTNLVRVDVSARNPGKVQAALTENRRALSEL 143
           +G++++E+ +  + F P                VRVD++++  GK++A L  N    SE+
Sbjct: 93  SGSYVVEIVSPSFRFEP----------------VRVDITSK--GKMRARLV-NYIKTSEV 152

Query: 144 VLEPL-------RDEEYYESREPFSIMSVVKSPMGLMVGFMLIVVFLMPKLMENIDPEEM 203
           + +P            Y+  RE +     + +PM +M+   L+++ L+PK++   DP EM
Sbjct: 153 IRQPYPLQIRAGGPHTYFMKRETWGWTDFLMNPMVMMMVLPLLIIVLLPKVVNTNDP-EM 196

Query: 204 RRAQEEMRN 206
           R+  E+  N
Sbjct: 213 RKEMEQSMN 196

BLAST of Carg02867 vs. ExPASy Swiss-Prot
Match: Q0IHY5 (ER membrane protein complex subunit 7 OS=Xenopus tropicalis OX=8364 GN=emc7 PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 2.3e-10
Identity = 62/212 (29.25%), Postives = 105/212 (49.53%), Query Frame = 0

Query: 2   RSQVFILLLSLQLFFSLLSPSHAISPGSG-DGYTIYGRVKISRNALKEYGLPGKISNIKV 61
           RS    LL  LQL  + L     +SPGSG D + + GR  +     +++     ++  +V
Sbjct: 13  RSLSLWLLALLQLCSADLD---VLSPGSGSDKFKVEGRAVVPGVRPQDW-----VNTARV 72

Query: 62  ILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSPVGFLYHPSGISFSTNLVRVD 121
           +++G + V FLR DG F  ++VP+G++++EV +  + F P                VRVD
Sbjct: 73  LVDGEEHVGFLRTDGSFVVHDVPSGSYVVEVISPAHRFEP----------------VRVD 132

Query: 122 VSARNPGKVQAALTENRRALSELVLEPL-------RDEEYYESREPFSIMSVVKSPMGLM 181
           ++++  GK++A    N    SE+V  P            Y+  RE +     + +PM +M
Sbjct: 133 ITSK--GKMRARYV-NHIKTSEVVRLPYPLQMKSSGPPSYFIKRETWGWTDFLMNPMVMM 192

Query: 182 VGFMLIVVFLMPKLMENIDPEEMRRAQEEMRN 206
           +   L++  L+PK++   DP EMRR  E+  N
Sbjct: 193 MVLPLLIFVLLPKVVNTSDP-EMRREMEQSMN 196

BLAST of Carg02867 vs. ExPASy Swiss-Prot
Match: Q9EP72 (ER membrane protein complex subunit 7 OS=Mus musculus OX=10090 GN=Emc7 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 4.0e-10
Identity = 62/209 (29.67%), Postives = 102/209 (48.80%), Query Frame = 0

Query: 5   VFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGK-ISNIKVILN 64
           V +LLLS     S +  + A  PG G G  +  R KI   A+     P   IS  +V+++
Sbjct: 11  VLLLLLSGDAHSSEVPGAAAEGPG-GSGVGLGDRFKIEGRAVVPGVKPQDWISAARVLVD 70

Query: 65  GGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSPVGFLYHPSGISFSTNLVRVDVSA 124
           G + V FL+ DG F  +++P+G++++EV +  Y F P                VRVD+++
Sbjct: 71  GEEHVGFLKTDGSFVVHDIPSGSYVVEVISPAYKFDP----------------VRVDITS 130

Query: 125 RNPGKVQAALTENRRALSELVLEPL-------RDEEYYESREPFSIMSVVKSPMGLMVGF 184
           +  GK++A    N    SE+V  P            Y+  RE +     + +PM +M+  
Sbjct: 131 K--GKMRARYV-NYIKTSEVVRLPYPLQMKSSGPPSYFIKRESWGWTDFLMNPMVMMMVL 190

Query: 185 MLIVVFLMPKLMENIDPEEMRRAQEEMRN 206
            L++  L+PK++   DP +MRR  E+  N
Sbjct: 191 PLLIFVLLPKVVNTSDP-DMRREMEQSMN 198

BLAST of Carg02867 vs. ExPASy Swiss-Prot
Match: Q4R5V2 (ER membrane protein complex subunit 7 OS=Macaca fascicularis OX=9541 GN=EMC7 PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 6.8e-10
Identity = 61/209 (29.19%), Postives = 98/209 (46.89%), Query Frame = 0

Query: 5   VFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGK-ISNIKVILN 64
           V +LLL      S   P  A     G G  I  R KI   A+     P   IS  +V+++
Sbjct: 11  VLLLLLLSGDVQSSEVPGAAAEGSGGSGVGIGDRFKIEGRAVVPGVKPQDWISAARVLVD 70

Query: 65  GGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSPVGFLYHPSGISFSTNLVRVDVSA 124
           G + V FL+ DG F  +++P+G++++EV +  Y F P                VRVD+++
Sbjct: 71  GEEHVGFLKTDGSFVVHDIPSGSYVVEVVSPAYRFDP----------------VRVDITS 130

Query: 125 RNPGKVQAALTENRRALSELVLEPL-------RDEEYYESREPFSIMSVVKSPMGLMVGF 184
           +  GK++A    N    SE+V  P            Y+  RE +     + +PM +M+  
Sbjct: 131 K--GKMRARYV-NHIKTSEVVRLPYPLQMKSSGPPSYFIKRESWGWTDFLMNPMVMMMVL 190

Query: 185 MLIVVFLMPKLMENIDPEEMRRAQEEMRN 206
            L++  L+PK++   DP +MRR  E+  N
Sbjct: 191 PLLIFVLLPKVVNTSDP-DMRREMEQSMN 199

BLAST of Carg02867 vs. ExPASy TrEMBL
Match: A0A6J1IMV4 (ER membrane protein complex subunit 7 homolog OS=Cucurbita maxima OX=3661 GN=LOC111476661 PE=4 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 4.9e-104
Identity = 205/222 (92.34%), Postives = 206/222 (92.79%), Query Frame = 0

Query: 1   MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV 60
           MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV
Sbjct: 1   MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV 60

Query: 61  ILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSPVGFLYHPSGISFSTNLVRVD 120
           ILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSP                VRVD
Sbjct: 61  ILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSP----------------VRVD 120

Query: 121 VSARNPGKVQAALTENRRALSELVLEPLRDEEYYESREPFSIMSVVKSPMGLMVGFMLIV 180
           VSARNPGKVQAALTENRRALSELVLEPLRDEEYYE+REPFSIMSVVKSPMGLMVGFMLIV
Sbjct: 121 VSARNPGKVQAALTENRRALSELVLEPLRDEEYYETREPFSIMSVVKSPMGLMVGFMLIV 180

Query: 181 VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS 223
           VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS
Sbjct: 181 VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS 206

BLAST of Carg02867 vs. ExPASy TrEMBL
Match: A0A6J1F5W6 (ER membrane protein complex subunit 7 homolog OS=Cucurbita moschata OX=3662 GN=LOC111442638 PE=4 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 4.2e-103
Identity = 204/222 (91.89%), Postives = 205/222 (92.34%), Query Frame = 0

Query: 1   MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV 60
           MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV
Sbjct: 1   MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV 60

Query: 61  ILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSPVGFLYHPSGISFSTNLVRVD 120
           ILNGGQRVTFLRPDGYFSFY+VPAGTHLLEVAALGYFFSP                VRVD
Sbjct: 61  ILNGGQRVTFLRPDGYFSFYDVPAGTHLLEVAALGYFFSP----------------VRVD 120

Query: 121 VSARNPGKVQAALTENRRALSELVLEPLRDEEYYESREPFSIMSVVKSPMGLMVGFMLIV 180
           VSARNPGKVQAALTENRRALSELVLEPLRDEEYYE REPFSIMSVVKSPMGLMVGFMLIV
Sbjct: 121 VSARNPGKVQAALTENRRALSELVLEPLRDEEYYEIREPFSIMSVVKSPMGLMVGFMLIV 180

Query: 181 VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS 223
           VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS
Sbjct: 181 VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS 206

BLAST of Carg02867 vs. ExPASy TrEMBL
Match: A0A6J1CCD4 (ER membrane protein complex subunit 7 homolog OS=Momordica charantia OX=3673 GN=LOC111009919 PE=4 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 4.4e-97
Identity = 190/220 (86.36%), Postives = 198/220 (90.00%), Query Frame = 0

Query: 1   MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV 60
           MRS+VFILLLS+Q++ SLL+PSHAISPGSGDGYTIYGRVKI  N +K YGLPGKISNIKV
Sbjct: 1   MRSKVFILLLSIQIYLSLLTPSHAISPGSGDGYTIYGRVKIPSNGIKGYGLPGKISNIKV 60

Query: 61  ILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSPVGFLYHPSGISFSTNLVRVD 120
           ILNGGQRVTFLRPDGYFSFYNVPAGTHL+EVAALGYFFSP                VRVD
Sbjct: 61  ILNGGQRVTFLRPDGYFSFYNVPAGTHLIEVAALGYFFSP----------------VRVD 120

Query: 121 VSARNPGKVQAALTENRRALSELVLEPLRDEEYYESREPFSIMSVVKSPMGLMVGFMLIV 180
           VSARNPGKVQAALTENRRALSELVLEPLRDEEYYE REPFSIMSVVKSPMGLMVGFMLIV
Sbjct: 121 VSARNPGKVQAALTENRRALSELVLEPLRDEEYYEVREPFSIMSVVKSPMGLMVGFMLIV 180

Query: 181 VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQ 221
           VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAG+
Sbjct: 181 VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGR 204

BLAST of Carg02867 vs. ExPASy TrEMBL
Match: A0A6J1FS36 (ER membrane protein complex subunit 7 homolog OS=Cucurbita moschata OX=3662 GN=LOC111446728 PE=4 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 4.4e-97
Identity = 192/222 (86.49%), Postives = 199/222 (89.64%), Query Frame = 0

Query: 1   MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV 60
           MRSQ  ILLL LQLFFSLLSPSHAIS GSGDGYTIYGRVKI+ NALK YGLPGKISNIKV
Sbjct: 1   MRSQASILLLLLQLFFSLLSPSHAISTGSGDGYTIYGRVKIAGNALKGYGLPGKISNIKV 60

Query: 61  ILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSPVGFLYHPSGISFSTNLVRVD 120
           ILNGGQR+TFLRPDGYFSFYNVPAGTHL+EVAALGYFFSP                VRVD
Sbjct: 61  ILNGGQRITFLRPDGYFSFYNVPAGTHLIEVAALGYFFSP----------------VRVD 120

Query: 121 VSARNPGKVQAALTENRRALSELVLEPLRDEEYYESREPFSIMSVVKSPMGLMVGFMLIV 180
           VSARNPGKVQAALTENRRALSELVLEPLRDEEYYE REPFSIMSVVKSPMGLM+GFM++V
Sbjct: 121 VSARNPGKVQAALTENRRALSELVLEPLRDEEYYEIREPFSIMSVVKSPMGLMMGFMILV 180

Query: 181 VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS 223
           VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAG+SS
Sbjct: 181 VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGRSS 206

BLAST of Carg02867 vs. ExPASy TrEMBL
Match: A0A6J1J2W1 (ER membrane protein complex subunit 7 homolog OS=Cucurbita maxima OX=3661 GN=LOC111480849 PE=4 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 4.9e-96
Identity = 189/222 (85.14%), Postives = 199/222 (89.64%), Query Frame = 0

Query: 1   MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV 60
           MRSQ  +LLL LQLFFSLLSPSHAIS GSGDGYTIYGRVKI+ NALK YGLPGKISNIKV
Sbjct: 1   MRSQASVLLLLLQLFFSLLSPSHAISTGSGDGYTIYGRVKIAGNALKGYGLPGKISNIKV 60

Query: 61  ILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSPVGFLYHPSGISFSTNLVRVD 120
           IL+GGQR+T+LRPDGYFSFYNVPAGTHL+EVAALGYFFSP                VRVD
Sbjct: 61  ILDGGQRITYLRPDGYFSFYNVPAGTHLIEVAALGYFFSP----------------VRVD 120

Query: 121 VSARNPGKVQAALTENRRALSELVLEPLRDEEYYESREPFSIMSVVKSPMGLMVGFMLIV 180
           VSARNPGKVQAALTENRRALSELVLEPLRDEEYYE REPFSIMSVVKSPMGLM+GFM++V
Sbjct: 121 VSARNPGKVQAALTENRRALSELVLEPLRDEEYYEIREPFSIMSVVKSPMGLMMGFMILV 180

Query: 181 VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQSS 223
           VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAG+SS
Sbjct: 181 VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGRSS 206

BLAST of Carg02867 vs. TAIR 10
Match: AT2G25310.1 (Protein of unknown function (DUF2012) )

HSP 1 Score: 263.5 bits (672), Expect = 1.6e-70
Identity = 140/221 (63.35%), Postives = 170/221 (76.92%), Query Frame = 0

Query: 1   MRSQVFILLLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKV 60
           +RS   +  + LQ+ F   + +   S GS D YTI GRVKI  + +   G   K SN+KV
Sbjct: 7   IRSSPTLAFIVLQICFIFFASTFPCSSGSEDSYTITGRVKIPPSNV--IGHIAKFSNVKV 66

Query: 61  ILNGGQRVTFLRPDGYFSFYNVPAGTHLLEVAALGYFFSPVGFLYHPSGISFSTNLVRVD 120
           ILNGGQ++TFLRPDGYF+F+ VPAGTHL+EV+A+GYFFSP                VRVD
Sbjct: 67  ILNGGQKITFLRPDGYFTFHEVPAGTHLIEVSAMGYFFSP----------------VRVD 126

Query: 121 VSARNPGKVQAALTENRRALSELVLEPLRDEEYYESREPFSIMSVVKSPMGLMVGFMLIV 180
           VSAR+ GKVQA LTE RR+L+ELVLEPL++E+YYE REPF+IMS+VKSPMGLMVGFM++V
Sbjct: 127 VSARHRGKVQATLTETRRSLTELVLEPLKEEQYYEIREPFNIMSIVKSPMGLMVGFMVVV 186

Query: 181 VFLMPKLMENIDPEEMRRAQEEMRNQGVPTLSSLLPGAGQS 222
           VFLMPKLMENIDPEEM++AQEEMR QGVP+L+SLLPGAG S
Sbjct: 187 VFLMPKLMENIDPEEMKQAQEEMRRQGVPSLTSLLPGAGAS 209

BLAST of Carg02867 vs. TAIR 10
Match: AT4G32130.1 (Protein of unknown function (DUF2012) )

HSP 1 Score: 258.8 bits (660), Expect = 3.9e-69
Identity = 137/210 (65.24%), Postives = 162/210 (77.14%), Query Frame = 0

Query: 9   LLSLQLFFSLLSPSHAISPGSGDGYTIYGRVKISRNALKEYGLPGKISNIKVILNGGQRV 68
           L++  LFF   + +  IS GS D YTI GRV++  + +   G   K SNIKVILNGGQ V
Sbjct: 10  LIAFSLFFFFFASTLPISSGSEDSYTITGRVRVPASTV--IGHAAKFSNIKVILNGGQHV 69

Query: 69  TFLRPDGYFSFYNVPAGTHLLEVAALGYFFSPVGFLYHPSGISFSTNLVRVDVSARNPGK 128
           TFLRPDGYF+F+ VPAGTHL+EV ALGYFFSP                VRVDVSAR+ GK
Sbjct: 70  TFLRPDGYFTFHKVPAGTHLIEVYALGYFFSP----------------VRVDVSARHRGK 129

Query: 129 VQAALTENRRALSELVLEPLRDEEYYESREPFSIMSVVKSPMGLMVGFMLIVVFLMPKLM 188
           VQA LTE RR+L+ELVLEPLR E+YYE REPFS+MS+VKSPMGLMVGFM++VVFLMPKLM
Sbjct: 130 VQATLTETRRSLTELVLEPLRAEQYYEMREPFSVMSIVKSPMGLMVGFMVVVVFLMPKLM 189

Query: 189 ENIDPEEMRRAQEEMRNQGVPTLSSLLPGA 219
           ENIDPEEM+ AQE+MR+QGVP+L+SLLP +
Sbjct: 190 ENIDPEEMKSAQEQMRSQGVPSLTSLLPAS 201

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7024445.14.7e-117100.00ER membrane protein complex subunit 7-like protein [Cucurbita argyrosperma subsp... [more]
XP_022976199.11.0e-10392.34ER membrane protein complex subunit 7 homolog [Cucurbita maxima][more]
KAG6591557.12.3e-10392.34ER membrane protein complex subunit 7-like protein, partial [Cucurbita argyrospe... [more]
XP_022935856.18.6e-10391.89ER membrane protein complex subunit 7 homolog [Cucurbita moschata][more]
XP_023535034.11.1e-10291.89ER membrane protein complex subunit 7 homolog [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q8VY975.5e-6865.24ER membrane protein complex subunit 7 homolog OS=Arabidopsis thaliana OX=3702 GN... [more]
Q5TYV02.8e-1127.51ER membrane protein complex subunit 7 OS=Danio rerio OX=7955 GN=emc7 PE=2 SV=1[more]
Q0IHY52.3e-1029.25ER membrane protein complex subunit 7 OS=Xenopus tropicalis OX=8364 GN=emc7 PE=2... [more]
Q9EP724.0e-1029.67ER membrane protein complex subunit 7 OS=Mus musculus OX=10090 GN=Emc7 PE=1 SV=1[more]
Q4R5V26.8e-1029.19ER membrane protein complex subunit 7 OS=Macaca fascicularis OX=9541 GN=EMC7 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1IMV44.9e-10492.34ER membrane protein complex subunit 7 homolog OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1F5W64.2e-10391.89ER membrane protein complex subunit 7 homolog OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1CCD44.4e-9786.36ER membrane protein complex subunit 7 homolog OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1FS364.4e-9786.49ER membrane protein complex subunit 7 homolog OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1J2W14.9e-9685.14ER membrane protein complex subunit 7 homolog OS=Cucurbita maxima OX=3661 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT2G25310.11.6e-7063.35Protein of unknown function (DUF2012) [more]
AT4G32130.13.9e-6965.24Protein of unknown function (DUF2012) [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019008Domain of unknown function DUF2012PFAMPF09430DUF2012coord: 55..173
e-value: 1.7E-18
score: 66.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 197..222
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 205..222
NoneNo IPR availablePANTHERPTHR13605:SF7ER MEMBRANE PROTEIN COMPLEX SUBUNIT 7 HOMOLOGcoord: 11..220
IPR039163ER membrane protein complex subunit 7PANTHERPTHR13605UNCHARACTERIZEDcoord: 11..220
IPR013784Carbohydrate-binding-like foldSUPERFAMILY49452Starch-binding domain-likecoord: 31..100

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg02867-RACarg02867-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0072546 EMC complex
molecular_function GO:0030246 carbohydrate binding