Carg02850 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg02850
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionbrassinosteroid-related acyltransferase 1-like
LocationCarg_Chr09: 2030568 .. 2035561 (+)
RNA-Seq ExpressionCarg02850
SyntenyCarg02850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCACTCCCCATGTTTCTCTGACAAAATCTCTTACTGTCTTCCCCAAAACCTTAAACCCCTCGAAACTTTTGGAGCTCTCAAATTTAGACAGGCAGTGTCCTTTACATATGTACCTTATTTTCTTCTACAATCCTTCTTCTATTTACACTAACTCATCACTTGATTCAGTGTTCACCAAACTGAAGATTGGTCTAGAAGAAACGTTGTCGGTCTGGTCCCCGGCAGCTGGTCGGTTGGAGCCGAACCCGGCGGATGGCAAGCTGAACCTCCGGTGCGACAACCGCGGTGCGATTCTAGCTCGGGCGGTCACGGATGTGAAGATATCAGAACTTGGGGATCTCTCAAATTATAATGAGTTCTTTGAGGAGTTGGTTCATAAACCTGTTTTGGATGGGGATTTTTGTGAGTTCCCTTTGGTTGTGGCTCAGGTGAGAGGCTGTTTAAATTTTTTGTTGTCAAATTTTACCTTAAAAACATGGTTTTTATTGGTCAGATTTTATTGTATGGGACAAGGAGACAGAGAGACAAAGAGACAAGCTTTGGATTACACAATGAACACATTCCACAGTCTGTAAAGGCCAATTATTTTTAATAATATTGCATTAAAAATTTAGGCTAAATTAATAAAAACATCCAATCTTTTGTAGTTTTAAAAATATAAGCGTTTGGAAAAAAAAAAATTCAAAAAATTTTTACGGTTAGTTTTGAACGGGAACGTTAGTATTTTGTTTAAAAAATACGTTTGAATCTTACGAAAAATGTTTTATTAATACTCTTATATCTTTTAAACCGTTAGTGCCGACTCATTCTTCAACAGGCATACGGCCAAAATCCTAGTATCCTCCTACTGCTTGGAAGCTTACGGTGTCGTGAAAATTTGTATGTATTTTTGAAATATGGTACTAACGGTTTCACTCATAGGTGACAAGATTTGGGTGCGGAGGATACTCCATAGGAACTGGAATAAGCCATTCATTATTCGATGGACCAGCTGCATACGAGTTTCTAAGTGCATGGGCGACAAAGTCAGCCATTTTCAAGGACGACAAGGAGCAATATGGGGTTGAGTTGCAGAAGCCCGTGCATGAAAGGGGGAGGCTTTTGATGGGAAGCGTCCCGAATCGGTGCTCGGGGAAGCGATCGGCAGCTGGGTCGGGAGCGGGGGCGGTGGCAATCGATCATTTGTACAAGTTGATAATGCAAAGTGCCATGGGGAATGAGGAAATGGAGAAAAAGGAGTATGGTTGTAGAACACTTCGAGTGAGCAGAGGGATGATTGAGACGTTGAAGAAGAAGGGTTTTGGGGAAAATGGTGGCTTTCCTTGTTCTTCATTTGATGTTATTGCTGCTCATCTATGGAAGGTACTTCTAACTTATTTTCAAAACTAATGGGAATGTTCTTACCGGCTAAGAAAAGGTTTTCACACTTTTATAAAAAATGTTTCGTTCTTTTCCTCAACCAACGCAGGATCTCTCAATCTACCCCCTTCGTGGCCCAGCATCCTTGCTGACACACTGTCTCGTGTCCATCCCCTTTCGAGGCCCAACGTCTTTGCTGACACACCGCCTCGTGTCCACCTCCCTTCGGAGCTCAGCCTCCATGCTGGCACATTGCCCAATGTCTGGTTCTGATACTGTTTGTAACAACTCAAGCCCCCATAGCAGATATTGTCCTTTTCGGGCTTTTCTTTTCAGGCTTCTTCTCAAGGTTTTTAAAACAACTAGGGAGAGGTTCCCACACCCGCATAAAAAATGTTTCGTTCTCCTTCTCAACCGATGTGGGATCTCACAATCCACCCCCTTTGGGGTCCAATGTCCTTATTGGCACACCGCCTTGTGTCCACCACCTTCGGGGCTCAACCTCCACGCTGACACATCGCTTGATGTCTAGTTCTAATACCATTTATAATAGCTCAAGCTCACTGTTAGCTGAGATTGTCCTATTTTGGTTTTTCCTCAAGGTTTTTAAAACGCGTCTGTCAATGAGAGATTTCCACACCTTTACGAAGAATGTTTCATTCTCCTCCTCAACCGATGTAGAATCTCACACCCAAATTGATTGTGGAGGGTATCATTTGAAGTTAATTAATGTGTGTTTGCATGGATTTGTTGTTATTTGGAATAATTAAGTTAATTAAGTTAGGCAAATTGGTTGGAGATTTTGATGTCTATTATTGTGAGTACAGGCAAGGACCAAGGCTCTGTCTGTGAGCAAAGAAAAGATGGTGTGCTTACAATTCGCAGTGGACGCCAGGAACAAGCTGAAGCCTCCTCTGCCCAAAGGCTTCAGCGGCAACTCCTTTGTGCTCGCTTCTGTGGCCTTACCTGCAAAACAATTAGAGGAAGAAAGCTACAAAACAATAATCGAGATGATTCGAGACGCCAAGTCTCAAGTCGACGACGACTATGTCAACGCATACCAACTCGGCCTTGATGGCCCTCAAGCTTCACTTCCTCCTCTCAAGGAGCTCACTGTCATATCAGATTGGACCAGAATGCCATTCCATAAGATTGAGTTCTTACATGGAGAAGCCGCCTATGCATCTCCTTTGAAGCCACCGATCCTCGACGTCGCTTACTTTCTGCAGAACCCCGCAGATAGCCAAGCGATCGATGTTCGGGTCACTTTGCTCTCGCAAACTCTTGATGCTTTTTCAACCTACTTTTTGACTAGCATGCAGTAAGCTTCACAAAGTTTGAATAGCATAAGGGTTTGTTCATTACCATGAAGAACAATGTCATTTCTGCATAGAATAAGAGAGCCCGAGCGAGCTTTTCTGATCTAATCCAAACTAGATATCGATCCGAAAGTCAACAAAAGACGAAACAAATCACTTATAGTATAGCGAAGCAAAATCTTTACCCATATGAATTACAATGTACAATTGTTGGCCAACATCAATAGAAATAAACAGTCATGGAATCAAAATGAGAAACACAGACTTCCTTAACAGATTATGTAGGAAGCTAAATAAGAACATGGTCAGAGAGCTAAACCTGCAGGAAGTAAATACATTAAAATCTGCTTGAGGCAAAACTAGATAACGCTCGCTGTCGACTCGAGAATTCGTTTTTCAGGTAGGTTGGGCTTCACATGCATGTTCCAATGGGATAATTATCACATCCAAAGTCCCCTTCCAATAATTTTCCATCATCTTGTTCCTGCAGAGTATCGTATTGTTACTGTAACGAACACCAATGCTTCGCATAAATGAAGCTCGGATGAAAATAACATTGACCGTATTGAGTTTTGGTCAACATCGAAAATGAAAAAAAAAAAAAGAATAAATATTAATAAGGAAATACCCTTCCATGCAATTCTAGGATATTCAGATATCATATCAAATGGGAAATGATACCTACTTCATTAGATACAATCACATAAGGGGAAAGAAATTGTAGGATGCGCAGGAAAAGAGCTATCAGTCTTTCTTCACTGCTAAGCCTTGATGTAACGCCTCAAGCCACCGTTAGCAGATATTGTTCGTTTTAGCCCGTTACGTATCGCCGTCGGCTTTACGGTTTTAAAACGCGTCTACTAGGGACAGGTTTCCACACCCTTATAAGGAATGTTTCGTTCCCCTCTCCAATTGATGTGAGATCTCACAATACATCCCCCTTAGAGACACAGCGTTCTCGCTGGCAAACCACCCAGTGTCTGGCTTTGAACCATTTGTAATAGCCCAAGTCACCGCTAGTAGATATTGTCCATTTTAGCCCGTTACGTATAGCCATCAGCCTCACAATTTTAAAAGACGTCTGCTAGGGAGAGGTTTCCACACTCTTGTAAAAAATGTTTCGTTCCCCTCTCCAACCAATGTGGAATCTCACACTTGTAGACTAAGTTCAATGAATGAAGCCTAAAAACACTAACAAGACAAAAGAGAAAGAAGTTACCTCTTTCATCATGGCACAAGCTATGTCTTCCAAGGAGCCGCAGTTACTTGAAGAGAATCTGGGTTGCATCTTTTGCTGATGATCGTACAGGTAACCTTGTTTCAACATGAGCGATGTCTCCATTGTGGATTTCTTGAATCTATCATGTTCCATCATTGCATGATCAAGAACAATTGCTTGATTAGTCACATTGAAATCCTTGCTACCAGAGAAGGTGTTCTGATTTTCCAAATGCTTGGGCAAAGTCTCTGCAGCACCATTCACTGGTATCAAAGAATTTGATGAACTACAGGCCATAGTTGTGTGGCAATAGGCATCCTGTTTGTGATCATTCCAATCTTGAACAGGAACCTTTGAGATCATTTGATTATATCTGTTGCTAGTTGTGGCTCCTTGTCGTTGAGAATCTGTTCTCGAATCATGGGACGGAGTAGATATTGAAGTAATAGACGAAATGTCAGAAAGACAATTTCCTATATGCAATGCACTGGTAGTATTGTCTCTCAACTGACTATGGTAGGAAGTAGCAATATTCGAAAGGTTGCTAGATGGAATTGTATTCAAGTGGACCCGAGATGAATGAACAGCTTTCGGCCAATTCTCACCAGTGCATCTAAGATTATCGAGCGAAGGACGTGAGCTTTCGGAAGTTGAAGATAGTACCGAAATCGAAGATTGAGATCCAAATTTTCTGCCAACATTCACTTCATGATGCTGAACTTCTGAAATCATGGGATCATTAGCTAATCCTAAAAGAGGATTACTCAAAGTTCCAGAAGACATGTTTGCATTTGGTAGGCCAATGGAGGCAGGAAATACTGTTCTCTCATCAAAAGCAGAAGAGAGATTGTCCACATGGGTCCCGTGCTTAACATGTTGTAGTTGATCAAGTTCTGATGGCTCAGGAATTCCCTGGAAAATATTCCCACTGCGGTTTCCAGAAAGCATGATTGGCTGAAATGTGGATTGATCATTGTTTGAATTGTTCATATTACCCAGTTTAACTGTTCCTGGGGACGAAAGGCCGTGCATACCCATACAAGCAGGAGTATTCAATCGACCGAGCATCCCAGTCGTTGGGATGGATCTATAA

mRNA sequence

ATGGCCACTCCCCATGTTTCTCTGACAAAATCTCTTACTGTCTTCCCCAAAACCTTAAACCCCTCGAAACTTTTGGAGCTCTCAAATTTAGACAGGCAGTGTCCTTTACATATGTACCTTATTTTCTTCTACAATCCTTCTTCTATTTACACTAACTCATCACTTGATTCAGTGTTCACCAAACTGAAGATTGGTCTAGAAGAAACGTTGTCGGTCTGGTCCCCGGCAGCTGGTCGGTTGGAGCCGAACCCGGCGGATGGCAAGCTGAACCTCCGGTGCGACAACCGCGGTGCGATTCTAGCTCGGGCGGTCACGGATGTGAAGATATCAGAACTTGGGGATCTCTCAAATTATAATGAGTTCTTTGAGGAGTTGGTTCATAAACCTGTTTTGGATGGGGATTTTTGTGAGTTCCCTTTGGTTGTGGCTCAGTTTCTAAGTGCATGGGCGACAAAGTCAGCCATTTTCAAGGACGACAAGGAGCAATATGGGGTTGAGTTGCAGAAGCCCGTGCATGAAAGGGGGAGGCTTTTGATGGGAAGCGTCCCGAATCGGTGCTCGGGGAAGCGATCGGCAGCTGGGTCGGGAGCGGGGGCGGTGGCAATCGATCATTTGTACAAGTTGATAATGCAAAGTGCCATGGGGAATGAGGAAATGGAGAAAAAGGAGTATGGTTGTAGAACACTTCGAGTGAGCAGAGGGATGATTGAGACGTTGAAGAAGAAGGGTTTTGGGGAAAATGTACAGGCAAGGACCAAGGCTCTGTCTGTGAGCAAAGAAAAGATGGTGTGCTTACAATTCGCAGTGGACGCCAGGAACAAGCTGAAGCCTCCTCTGCCCAAAGGCTTCAGCGGCAACTCCTTTGTGCTCGCTTCTGTGGCCTTACCTGCAAAACAATTAGAGGAAGAAAGCTACAAAACAATAATCGAGATGATTCGAGACGCCAAGTCTCAAGTCGACGACGACTATGTCAACGCATACCAACTCGGCCTTGATGGCCCTCAAGCTTCACTTCCTCCTCTCAAGGAGCTCACTGTCATATCAGATTGGACCAGAATGCCATTCCATAAGATTGAGTTCTTACATGGAGAAGCCGCCTATGCATCTCCTTTGAAGCCACCGATCCTCGACGTCGCTTACTTTCTGCAGAACCCCGCAGATAGCCAAGCGATCGATGTTCGGGTCACTTTGCTCTCGCAAACTCTTGATGCTTTTTCAACCTACTTTTTGACTAGCATGCATTTAACTGTTCCTGGGGACGAAAGGCCGTGCATACCCATACAAGCAGGAGTATTCAATCGACCGAGCATCCCAGTCGTTGGGATGGATCTATAA

Coding sequence (CDS)

ATGGCCACTCCCCATGTTTCTCTGACAAAATCTCTTACTGTCTTCCCCAAAACCTTAAACCCCTCGAAACTTTTGGAGCTCTCAAATTTAGACAGGCAGTGTCCTTTACATATGTACCTTATTTTCTTCTACAATCCTTCTTCTATTTACACTAACTCATCACTTGATTCAGTGTTCACCAAACTGAAGATTGGTCTAGAAGAAACGTTGTCGGTCTGGTCCCCGGCAGCTGGTCGGTTGGAGCCGAACCCGGCGGATGGCAAGCTGAACCTCCGGTGCGACAACCGCGGTGCGATTCTAGCTCGGGCGGTCACGGATGTGAAGATATCAGAACTTGGGGATCTCTCAAATTATAATGAGTTCTTTGAGGAGTTGGTTCATAAACCTGTTTTGGATGGGGATTTTTGTGAGTTCCCTTTGGTTGTGGCTCAGTTTCTAAGTGCATGGGCGACAAAGTCAGCCATTTTCAAGGACGACAAGGAGCAATATGGGGTTGAGTTGCAGAAGCCCGTGCATGAAAGGGGGAGGCTTTTGATGGGAAGCGTCCCGAATCGGTGCTCGGGGAAGCGATCGGCAGCTGGGTCGGGAGCGGGGGCGGTGGCAATCGATCATTTGTACAAGTTGATAATGCAAAGTGCCATGGGGAATGAGGAAATGGAGAAAAAGGAGTATGGTTGTAGAACACTTCGAGTGAGCAGAGGGATGATTGAGACGTTGAAGAAGAAGGGTTTTGGGGAAAATGTACAGGCAAGGACCAAGGCTCTGTCTGTGAGCAAAGAAAAGATGGTGTGCTTACAATTCGCAGTGGACGCCAGGAACAAGCTGAAGCCTCCTCTGCCCAAAGGCTTCAGCGGCAACTCCTTTGTGCTCGCTTCTGTGGCCTTACCTGCAAAACAATTAGAGGAAGAAAGCTACAAAACAATAATCGAGATGATTCGAGACGCCAAGTCTCAAGTCGACGACGACTATGTCAACGCATACCAACTCGGCCTTGATGGCCCTCAAGCTTCACTTCCTCCTCTCAAGGAGCTCACTGTCATATCAGATTGGACCAGAATGCCATTCCATAAGATTGAGTTCTTACATGGAGAAGCCGCCTATGCATCTCCTTTGAAGCCACCGATCCTCGACGTCGCTTACTTTCTGCAGAACCCCGCAGATAGCCAAGCGATCGATGTTCGGGTCACTTTGCTCTCGCAAACTCTTGATGCTTTTTCAACCTACTTTTTGACTAGCATGCATTTAACTGTTCCTGGGGACGAAAGGCCGTGCATACCCATACAAGCAGGAGTATTCAATCGACCGAGCATCCCAGTCGTTGGGATGGATCTATAA

Protein sequence

MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNEFFEELVHKPVLDGDFCEFPLVVAQFLSAWATKSAIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLYKLIMQSAMGNEEMEKKEYGCRTLRVSRGMIETLKKKGFGENVQARTKALSVSKEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQVDDDYVNAYQLGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDVAYFLQNPADSQAIDVRVTLLSQTLDAFSTYFLTSMHLTVPGDERPCIPIQAGVFNRPSIPVVGMDL
Homology
BLAST of Carg02850 vs. NCBI nr
Match: KAG7024428.1 (Brassinosteroid-related acyltransferase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 887.1 bits (2291), Expect = 6.2e-254
Identity = 444/444 (100.00%), Postives = 444/444 (100.00%), Query Frame = 0

Query: 1   MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFT 60
           MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFT
Sbjct: 1   MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFT 60

Query: 61  KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE 120
           KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE
Sbjct: 61  KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE 120

Query: 121 FFEELVHKPVLDGDFCEFPLVVAQFLSAWATKSAIFKDDKEQYGVELQKPVHERGRLLMG 180
           FFEELVHKPVLDGDFCEFPLVVAQFLSAWATKSAIFKDDKEQYGVELQKPVHERGRLLMG
Sbjct: 121 FFEELVHKPVLDGDFCEFPLVVAQFLSAWATKSAIFKDDKEQYGVELQKPVHERGRLLMG 180

Query: 181 SVPNRCSGKRSAAGSGAGAVAIDHLYKLIMQSAMGNEEMEKKEYGCRTLRVSRGMIETLK 240
           SVPNRCSGKRSAAGSGAGAVAIDHLYKLIMQSAMGNEEMEKKEYGCRTLRVSRGMIETLK
Sbjct: 181 SVPNRCSGKRSAAGSGAGAVAIDHLYKLIMQSAMGNEEMEKKEYGCRTLRVSRGMIETLK 240

Query: 241 KKGFGENVQARTKALSVSKEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQL 300
           KKGFGENVQARTKALSVSKEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQL
Sbjct: 241 KKGFGENVQARTKALSVSKEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQL 300

Query: 301 EEESYKTIIEMIRDAKSQVDDDYVNAYQLGLDGPQASLPPLKELTVISDWTRMPFHKIEF 360
           EEESYKTIIEMIRDAKSQVDDDYVNAYQLGLDGPQASLPPLKELTVISDWTRMPFHKIEF
Sbjct: 301 EEESYKTIIEMIRDAKSQVDDDYVNAYQLGLDGPQASLPPLKELTVISDWTRMPFHKIEF 360

Query: 361 LHGEAAYASPLKPPILDVAYFLQNPADSQAIDVRVTLLSQTLDAFSTYFLTSMHLTVPGD 420
           LHGEAAYASPLKPPILDVAYFLQNPADSQAIDVRVTLLSQTLDAFSTYFLTSMHLTVPGD
Sbjct: 361 LHGEAAYASPLKPPILDVAYFLQNPADSQAIDVRVTLLSQTLDAFSTYFLTSMHLTVPGD 420

Query: 421 ERPCIPIQAGVFNRPSIPVVGMDL 445
           ERPCIPIQAGVFNRPSIPVVGMDL
Sbjct: 421 ERPCIPIQAGVFNRPSIPVVGMDL 444

BLAST of Carg02850 vs. NCBI nr
Match: KAG6591543.1 (Brassinosteroid-related acyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 789.3 bits (2037), Expect = 1.8e-224
Identity = 409/455 (89.89%), Postives = 410/455 (90.11%), Query Frame = 0

Query: 1   MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFT 60
           MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFT
Sbjct: 1   MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFT 60

Query: 61  KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE 120
           KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE
Sbjct: 61  KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE 120

Query: 121 FFEELVHKPVLDGDFCEFPLVVAQ---------------------------FLSAWATKS 180
           FFEELVHKPVLDGDFCEFPLVVAQ                           FLSAWATKS
Sbjct: 121 FFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWATKS 180

Query: 181 AIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLYKLIMQSA 240
           AIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLYKLIMQSA
Sbjct: 181 AIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLYKLIMQSA 240

Query: 241 MGNEEMEKKEYGCRTLRVSRGMIETLKKKGFGEN---------------VQARTKALSVS 300
           MGNEEMEKKEYGCRTLRVSRGMIETLKKKGFGEN                +ARTKALSVS
Sbjct: 241 MGNEEMEKKEYGCRTLRVSRGMIETLKKKGFGENGGFPCSSFDVIAAHLWKARTKALSVS 300

Query: 301 KEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQ 360
           KEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASV LPAKQLEEESYKTIIEMIRDAKSQ
Sbjct: 301 KEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASVTLPAKQLEEESYKTIIEMIRDAKSQ 360

Query: 361 VDDDYVNAYQLGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV 414
           VDDDYVNAYQLGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV
Sbjct: 361 VDDDYVNAYQLGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV 420

BLAST of Carg02850 vs. NCBI nr
Match: XP_022936964.1 (brassinosteroid-related acyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 783.1 bits (2021), Expect = 1.3e-222
Identity = 406/456 (89.04%), Postives = 408/456 (89.47%), Query Frame = 0

Query: 1   MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFT 60
           MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFT
Sbjct: 1   MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFT 60

Query: 61  KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE 120
           KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE
Sbjct: 61  KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE 120

Query: 121 FFEELVHKPVLDGDFCEFPLVVAQ---------------------------FLSAWATKS 180
           FFEELVHKPVLDGDFCEFPLVVAQ                           FLSAWATKS
Sbjct: 121 FFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWATKS 180

Query: 181 AIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLYKLIMQSA 240
           AIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGK SAAGSGAGAVAIDHLYKLIMQSA
Sbjct: 181 AIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHLYKLIMQSA 240

Query: 241 MGNEEMEKKEYGCRTLRVSRGMIETLKKKGFGEN---------------VQARTKALSVS 300
           MGNEEMEKKEYGCRTLRVSRGMIETLKKK FGEN                +ARTKALSVS
Sbjct: 241 MGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFDVIAAHLWKARTKALSVS 300

Query: 301 KEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQ 360
           KEK+VCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQ
Sbjct: 301 KEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQ 360

Query: 361 VDDDYVNAYQLGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV 415
           VDDDYVNAYQ GLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV
Sbjct: 361 VDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV 420

BLAST of Carg02850 vs. NCBI nr
Match: XP_023535946.1 (brassinosteroid-related acyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 780.8 bits (2015), Expect = 6.3e-222
Identity = 404/455 (88.79%), Postives = 408/455 (89.67%), Query Frame = 0

Query: 1   MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFT 60
           MATPHVSLT+SL VFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFT
Sbjct: 1   MATPHVSLTQSLAVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFT 60

Query: 61  KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE 120
           KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILA+AVTDVKISELGDLSNYNE
Sbjct: 61  KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILAQAVTDVKISELGDLSNYNE 120

Query: 121 FFEELVHKPVLDGDFCEFPLVVAQ---------------------------FLSAWATKS 180
           FFEELVHKP LDGDFCEFPLVVAQ                           FLSAWATKS
Sbjct: 121 FFEELVHKPALDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWATKS 180

Query: 181 AIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLYKLIMQSA 240
           AIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLYKLIMQSA
Sbjct: 181 AIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLYKLIMQSA 240

Query: 241 MGNEEMEKKEYGCRTLRVSRGMIETLKKKGFGEN---------------VQARTKALSVS 300
           MGNEEMEKKEYGCRTLRVSRGMIETLKKK FGEN                +ARTKALSVS
Sbjct: 241 MGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFDVIAAHLWKARTKALSVS 300

Query: 301 KEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQ 360
           KEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQ
Sbjct: 301 KEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQ 360

Query: 361 VDDDYVNAYQLGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV 414
           VDDDYVNAYQLGLDGPQA+LPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV
Sbjct: 361 VDDDYVNAYQLGLDGPQATLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV 420

BLAST of Carg02850 vs. NCBI nr
Match: XP_022976161.1 (brassinosteroid-related acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 762.3 bits (1967), Expect = 2.3e-216
Identity = 398/455 (87.47%), Postives = 401/455 (88.13%), Query Frame = 0

Query: 1   MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFT 60
           MATPHVS T SLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSS YTNSSLDSVFT
Sbjct: 1   MATPHVSRTTSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSAYTNSSLDSVFT 60

Query: 61  KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE 120
           KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE
Sbjct: 61  KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE 120

Query: 121 FFEELVHKPVLDGDFCEFPLVVAQ---------------------------FLSAWATKS 180
           FFEELVHKPVLDGDFCEFPLVVAQ                           FLSAWATKS
Sbjct: 121 FFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWATKS 180

Query: 181 AIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLYKLIMQSA 240
           AIFKD+KEQ  VELQKPVHERGRLLMGSVPNRCSGKRSAAGSGA AVAIDHLYKLIMQSA
Sbjct: 181 AIFKDNKEQNEVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAEAVAIDHLYKLIMQSA 240

Query: 241 MGNEEMEKKEYGCRTLRVSRGMIETLKKKGFGEN---------------VQARTKALSVS 300
           MGNEEMEKKEYGCRTLRVSRGMIE LKKK FGEN                +ARTKALSVS
Sbjct: 241 MGNEEMEKKEYGCRTLRVSRGMIERLKKKAFGENGGFACSSFDVIAAHLWKARTKALSVS 300

Query: 301 KEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQ 360
           KEK VCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQ
Sbjct: 301 KEKTVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQ 360

Query: 361 VDDDYVNAYQLGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV 414
           VDDDYVNAYQLGLDGPQASLPPL ELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV
Sbjct: 361 VDDDYVNAYQLGLDGPQASLPPLNELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV 420

BLAST of Carg02850 vs. ExPASy Swiss-Prot
Match: Q9SZ58 (Brassinosteroid-related acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=BAT1 PE=2 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 2.1e-87
Identity = 201/466 (43.13%), Postives = 278/466 (59.66%), Query Frame = 0

Query: 1   MATPHVSLTKSLTVFPKTLN--PSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSV 60
           MAT  + + + L V+P+  N    KL+ LSNLDRQCPL MY +FFY  +   T    DSV
Sbjct: 5   MAT-RIDIIQKLNVYPRFQNHDKKKLITLSNLDRQCPLLMYSVFFYKNT---TTRDFDSV 64

Query: 61  FTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAV-TDVKISELGDLSN 120
           F+ LK+GLEET+SVW PAAGRL  +    KLN+RC++ GA++  AV T VK+SELGDL+ 
Sbjct: 65  FSNLKLGLEETMSVWYPAAGRLGLDGGGCKLNIRCNDGGAVMVEAVATGVKLSELGDLTQ 124

Query: 121 YNEFFEELVHKPVLDGDFCEFPLVVAQ---------------------------FLSAWA 180
           YNEF+E LV+KP LDGDF   PLVVAQ                           F+ AWA
Sbjct: 125 YNEFYENLVYKPSLDGDFSVMPLVVAQVTRFACGGYSIGIGTSHSLFDGISAYEFIHAWA 184

Query: 181 TKSAIFKDDKEQYGVELQ----KPVHERGRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLY 240
           + S I      +   + +    KPVH+R  LL+    NR + + +       A AI HLY
Sbjct: 185 SNSHIHNKSNSKITNKKEDVVIKPVHDRRNLLV----NRDAVRET------NAAAICHLY 244

Query: 241 KLIMQSAM------GNEEMEKKEYGCRTLRVSRGMIETLKKK---GF---------GENV 300
           +LI Q+ M       N E+    +  +T  ++   IE++KKK   GF             
Sbjct: 245 QLIKQAMMTYQEQNRNLELPDSGFVIKTFELNGDAIESMKKKSLEGFMCSSFEFLAAHLW 304

Query: 301 QARTKALSVSKEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQ-LEEESYKT 360
           +ART+AL + ++ MVCLQFAVD R + + PLP+GFSGN++VLASVA  A++ LEE + ++
Sbjct: 305 KARTRALGLRRDAMVCLQFAVDIRKRTETPLPEGFSGNAYVLASVASTARELLEELTLES 364

Query: 361 IIEMIRDAKSQVDDDYVNAYQLGLDGP-QASLPPLKELTVISDWTRMPFHKIEFLHG--E 411
           I+  IR+AK  +D  Y+N+Y   L G    +LPPLKELT+ISDWT+MPFH + F +G   
Sbjct: 365 IVNKIREAKKSIDQGYINSYMEALGGSNDGNLPPLKELTLISDWTKMPFHNVGFGNGGEP 424

BLAST of Carg02850 vs. ExPASy Swiss-Prot
Match: Q94CD1 (Omega-hydroxypalmitate O-feruloyl transferase OS=Arabidopsis thaliana OX=3702 GN=HHT1 PE=1 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 2.6e-21
Identity = 108/425 (25.41%), Postives = 177/425 (41.65%), Query Frame = 0

Query: 14  VFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFTKLKIGLEETLSVW 73
           V P++     L  LSNLD+   + +  I+ +         ++  +    K  L + L  +
Sbjct: 41  VKPESETRKGLYFLSNLDQNIAVIVRTIYCFKSEERGNEEAVQVI----KKALSQVLVHY 100

Query: 74  SPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYN-EFFEELVHKPVLD 133
            P AGRL  +P +GKL + C   G +   A  + K+ E+GD++  + E   +LV+  V  
Sbjct: 101 YPLAGRLTISP-EGKLTVDCTEEGVVFVEAEANCKMDEIGDITKPDPETLGKLVYDVVDA 160

Query: 134 GDFCEFPLVVAQ--------FLSAWATKSAIFKD----------DKEQYGVELQKPVHER 193
            +  E P V AQ        F+        +F             +   G+ L  P    
Sbjct: 161 KNILEIPPVTAQVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNSWGQVARGLPLTTPPFSD 220

Query: 194 GRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLY---KLIMQSAMGNEEMEKK------EYG 253
             +L    P +                I+ LY     + +S   + E  KK      E  
Sbjct: 221 RTILNARNPPKIENLHQEFEEIEDKSNINSLYTKEPTLYRSFCFDPEKIKKLKLQATENS 280

Query: 254 CRTLRVSRGMIETLKKKGFGENVQARTKALSVSKEKMVCLQFAVDARNKLKPPLPKGFSG 313
              L  S    E L    +    +ARTK+L +  ++   L FAVD R K +P LPKG+ G
Sbjct: 281 ESLLGNSCTSFEALSAFVW----RARTKSLKMLSDQKTKLLFAVDGRAKFEPQLPKGYFG 340

Query: 314 NSFVLASVALPAKQLEEESYKTIIEMIRDAKSQVDDDYVNAYQLGLDGPQASLPPLKELT 373
           N  VL +    A +L E+     + ++R+A   V D Y+ +     +  +A  P L    
Sbjct: 341 NGIVLTNSICEAGELIEKPLSFAVGLVREAIKMVTDGYMRSAIDYFEVTRAR-PSLSSTL 400

Query: 374 VISDWTRMPFHKIEFLHGEAAYASPLKPPILDVAYFLQNPADSQAIDVRVTLLSQTLDAF 411
           +I+ W+R+ FH  +F  GE   + P+  P  +V  FL +    ++I+V + L +  +D F
Sbjct: 401 LITTWSRLGFHTTDFGWGEPILSGPVALPEKEVTLFLSHGEQRRSINVLLGLPATAMDVF 455

BLAST of Carg02850 vs. ExPASy Swiss-Prot
Match: Q9FFQ7 (Fatty alcohol:caffeoyl-CoA acyltransferase OS=Arabidopsis thaliana OX=3702 GN=FACT PE=2 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 7.2e-19
Identity = 105/436 (24.08%), Postives = 184/436 (42.20%), Query Frame = 0

Query: 6   VSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFTKLKIG 65
           V+  + + V P +  P  L  LSNLD+   + +   +++  +S     S +  +  +K  
Sbjct: 9   VTRKEPVLVSPASETPKGLHYLSNLDQNIAIIVKTFYYFKSNS----RSNEESYEVIKKS 68

Query: 66  LEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDV---KISELGDLSNYNEFF 125
           L E L  + PAAGRL  +P +GK+ + C   G ++  A  +    KI +     +  E  
Sbjct: 69  LSEVLVHYYPAAGRLTISP-EGKIAVDCTGEGVVVVEAEANCGIEKIKKAISEIDQPETL 128

Query: 126 EELVHKPVLDGDFCEFPLVVAQ---------------------------FLSAWATKSAI 185
           E+LV+      +  E P VV Q                           FL++WA  +  
Sbjct: 129 EKLVYDVPGARNILEIPPVVVQVTNFKCGGFVLGLGMNHNMFDGIAAMEFLNSWAETAR- 188

Query: 186 FKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKRSAAG-----SGAGAVAIDHLYKLIM 245
                   G+ L  P      LL    P +     +        SG G +  D   KL+ 
Sbjct: 189 --------GLPLSVPPFLDRTLLRPRTPPKIEFPHNEFEDLEDISGTGKLYSDE--KLVY 248

Query: 246 QSAM-GNEEMEKKEYGCRTLRVSRGMIETLKKKGFGENVQARTKALSVSKEKMVCLQFAV 305
           +S + G E++E+ +    T   +    +TL     G   +AR +AL +  ++ + L FA 
Sbjct: 249 KSFLFGPEKLERLKIMAETRSTT---FQTLT----GFLWRARCQALGLKPDQRIKLLFAA 308

Query: 306 DARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQVDDDYVNAYQL 365
           D R++  P LPKG+SGN  V       A ++        + +++ A   V+D ++ +   
Sbjct: 309 DGRSRFVPELPKGYSGNGIVFTYCVTTAGEVTLNPLSHSVCLVKRAVEMVNDGFMRSAID 368

Query: 366 GLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDVAYFLQNPADSQ 406
             +  +A  P L    +I+ W ++ FH  +F  GE   + P+  P  +V  FL   +D++
Sbjct: 369 YFEVTRAR-PSLTATLLITSWAKLSFHTKDFGWGEPVVSGPVGLPEKEVILFLPCGSDTK 420

BLAST of Carg02850 vs. ExPASy Swiss-Prot
Match: A0A2R6PLK2 (Alcohol acyltransferase 9 OS=Actinidia chinensis var. chinensis OX=1590841 GN=AT9 PE=3 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.7e-15
Identity = 87/401 (21.70%), Postives = 161/401 (40.15%), Query Frame = 0

Query: 1   MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHM---YLIFFYNPSSIYTNSSLDS 60
           MA+    + K + V P    PS +L LS+LD Q  L     YL+ + +P  +      D 
Sbjct: 1   MASSVRLVKKPVLVAPVDPTPSTVLSLSSLDSQLFLRFPIEYLLVYASPHGV----DRDV 60

Query: 61  VFTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSN 120
              ++K  L  +L  + P AGR++  P    L++ C  +GA L  AV+D   S+      
Sbjct: 61  TAARVKAALARSLVPYYPLAGRVKTRPDSTGLDVVCQAQGAGLLEAVSDYTASDFQRAPR 120

Query: 121 YNEFFEELVHKPVLDGDFCEFPLVVAQFL-----------------------SAWATKSA 180
               + +L+   V    F   P +V Q                         S +    A
Sbjct: 121 SVAEWRKLLLVEV----FKVVPPLVVQLTWLSDGCVALGIGFSHCVIDGIGSSEFLNLFA 180

Query: 181 IFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLYKLIMQSAM 240
                + +      KPV +R  L      N  +                   + +  +++
Sbjct: 181 ELATSRAKLSEFQPKPVWDRQLLNSAGRTNLGTHPEFGRVPDLSGFVTRFAQERLSPTSI 240

Query: 241 GNEEMEKKEYGCRTLRVSRGMIETLKKKGF----GENVQARTKALSVSKEKMVCLQFAVD 300
             ++   KE   + + +S           F    G   ++  ++L++  ++++ L F+++
Sbjct: 241 TFDKTWLKE--LKNIAMSTSQAGEFPYTSFEVLSGHIWRSWARSLNLPAKQVLKLLFSIN 300

Query: 301 ARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQVDDDYVNAYQLG 360
            RN++KP LP G+ GN+FVL       K L E+      +++R AK +V D+Y       
Sbjct: 301 IRNRVKPSLPAGYYGNAFVLGCAQTSVKDLTEKGLGYCADLVRGAKERVGDEYAREVVES 360

Query: 361 LDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPL 372
           +  P+ + P    + +IS W+R+   +++F  G   +  P+
Sbjct: 361 VSWPRRASPDSVGVLIISQWSRLGLDRVDFGLGRPVHVGPI 391

BLAST of Carg02850 vs. ExPASy Swiss-Prot
Match: A0A068BIF1 (Alcohol acyltransferase 9 OS=Actinidia eriantha OX=165200 GN=AT9 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 2.8e-15
Identity = 104/459 (22.66%), Postives = 186/459 (40.52%), Query Frame = 0

Query: 1   MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHM---YLIFFYNPSSIYTNSSLDS 60
           MA+    + K + V P    PS +L LS+LD Q  L     YL+ + +P  +      D 
Sbjct: 1   MASSVRLVKKPVLVAPVDPTPSTVLSLSSLDSQLFLRFPIEYLLVYASPHGV------DR 60

Query: 61  VFT--KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDL 120
             T  ++K  L  +L  + P AGR++  P    L++ C  +GA L  AV+D   S+    
Sbjct: 61  AVTAARVKAALARSLVPYYPLAGRVKTRPDSTGLDVVCQAQGAGLLEAVSDYTASDFQRA 120

Query: 121 SNYNEFFEELVHKPVLDGDFCEFPLVVAQFL-----------------------SAWATK 180
                 + +L+   V    F   P +V Q                         S +   
Sbjct: 121 PRSVTEWRKLLLVEV----FKVVPPLVVQLTWLSDGCVALGVGFSHCVIDGIGSSEFLNL 180

Query: 181 SAIFKDDKEQYGVELQKPVHER------GRLLMGSVP------------NRCSGKRSAAG 240
            A     + +      KPV +R      GR  +G+ P             R + +R +  
Sbjct: 181 FAELATGRARLSEFQPKPVWDRHLLNSAGRTNLGTHPEFGRVPDLSGFVTRFTQERLSPT 240

Query: 241 SGAGAVAIDHLY-KLIMQSAMGNEEMEKKEYGCRTLRVSRGMIETLKKKGFGENVQARTK 300
           S    +  D  + K +   AM     +  E+   +  V  G I            ++  +
Sbjct: 241 S----ITFDKTWLKELKNIAMSTS--QPGEFPYTSFEVLSGHI-----------WRSWAR 300

Query: 301 ALSVSKEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIR 360
           +L++  ++++ L F+++ RN++KP LP G+ GN+FVL       K L E+      +++R
Sbjct: 301 SLNLPAKQVLKLLFSINIRNRVKPSLPAGYYGNAFVLGCAQTSVKDLTEKGLGYCADLVR 360

Query: 361 DAKSQVDDDYVNAYQLGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKP 413
            AK +V D+Y       +  P+ + P    + +IS W+R+   +++F  G      P+  
Sbjct: 361 GAKERVGDEYAREVVESVSWPRRASPDSVGVLIISQWSRLGLDRVDFGLGRPVQVGPICC 420

BLAST of Carg02850 vs. ExPASy TrEMBL
Match: A0A6J1F9T5 (brassinosteroid-related acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111443397 PE=3 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 6.1e-223
Identity = 406/456 (89.04%), Postives = 408/456 (89.47%), Query Frame = 0

Query: 1   MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFT 60
           MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFT
Sbjct: 1   MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFT 60

Query: 61  KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE 120
           KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE
Sbjct: 61  KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE 120

Query: 121 FFEELVHKPVLDGDFCEFPLVVAQ---------------------------FLSAWATKS 180
           FFEELVHKPVLDGDFCEFPLVVAQ                           FLSAWATKS
Sbjct: 121 FFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWATKS 180

Query: 181 AIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLYKLIMQSA 240
           AIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGK SAAGSGAGAVAIDHLYKLIMQSA
Sbjct: 181 AIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHLYKLIMQSA 240

Query: 241 MGNEEMEKKEYGCRTLRVSRGMIETLKKKGFGEN---------------VQARTKALSVS 300
           MGNEEMEKKEYGCRTLRVSRGMIETLKKK FGEN                +ARTKALSVS
Sbjct: 241 MGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFDVIAAHLWKARTKALSVS 300

Query: 301 KEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQ 360
           KEK+VCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQ
Sbjct: 301 KEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQ 360

Query: 361 VDDDYVNAYQLGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV 415
           VDDDYVNAYQ GLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV
Sbjct: 361 VDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV 420

BLAST of Carg02850 vs. ExPASy TrEMBL
Match: A0A6J1IG59 (brassinosteroid-related acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111476630 PE=3 SV=1)

HSP 1 Score: 762.3 bits (1967), Expect = 1.1e-216
Identity = 398/455 (87.47%), Postives = 401/455 (88.13%), Query Frame = 0

Query: 1   MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFT 60
           MATPHVS T SLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSS YTNSSLDSVFT
Sbjct: 1   MATPHVSRTTSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSAYTNSSLDSVFT 60

Query: 61  KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE 120
           KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE
Sbjct: 61  KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE 120

Query: 121 FFEELVHKPVLDGDFCEFPLVVAQ---------------------------FLSAWATKS 180
           FFEELVHKPVLDGDFCEFPLVVAQ                           FLSAWATKS
Sbjct: 121 FFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWATKS 180

Query: 181 AIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLYKLIMQSA 240
           AIFKD+KEQ  VELQKPVHERGRLLMGSVPNRCSGKRSAAGSGA AVAIDHLYKLIMQSA
Sbjct: 181 AIFKDNKEQNEVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAEAVAIDHLYKLIMQSA 240

Query: 241 MGNEEMEKKEYGCRTLRVSRGMIETLKKKGFGEN---------------VQARTKALSVS 300
           MGNEEMEKKEYGCRTLRVSRGMIE LKKK FGEN                +ARTKALSVS
Sbjct: 241 MGNEEMEKKEYGCRTLRVSRGMIERLKKKAFGENGGFACSSFDVIAAHLWKARTKALSVS 300

Query: 301 KEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQ 360
           KEK VCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQ
Sbjct: 301 KEKTVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQ 360

Query: 361 VDDDYVNAYQLGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV 414
           VDDDYVNAYQLGLDGPQASLPPL ELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV
Sbjct: 361 VDDDYVNAYQLGLDGPQASLPPLNELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV 420

BLAST of Carg02850 vs. ExPASy TrEMBL
Match: A0A6J1IT61 (brassinosteroid-related acyltransferase 1 OS=Cucurbita maxima OX=3661 GN=LOC111480377 PE=3 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 9.0e-174
Identity = 326/455 (71.65%), Postives = 364/455 (80.00%), Query Frame = 0

Query: 1   MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFT 60
           MAT HVS+TK+LTVFPKTLN  KLLELSNLDRQCPLH+YLIFFY PSS   +SSLDS+FT
Sbjct: 1   MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSS---SSSLDSIFT 60

Query: 61  KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE 120
            LKIGLEETLSVWSPAAGRLEPNPA+GKLNLRC+NRGAIL ++ T+++ISELGDLSNYNE
Sbjct: 61  NLKIGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYNE 120

Query: 121 FFEELVHKPVLDGDFCEFPLVVAQ---------------------------FLSAWATKS 180
           FFE+LV+KPVL+G+F EFPLVVAQ                           FLSAWA KS
Sbjct: 121 FFEKLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKS 180

Query: 181 AIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLYKLIMQSA 240
           AIFKD KE YGVEL  PVHERGRLLM     R S KR+   S +GAVAIDHLY+LIMQS 
Sbjct: 181 AIFKDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSV 240

Query: 241 MGNEEMEKKEYGCRTLRVSRGMIETLKKKGFGEN---------------VQARTKALSVS 300
            G EEM KKEY  RT R+SRGMIE LKK   GEN                +ARTKA+SVS
Sbjct: 241 TGKEEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVS 300

Query: 301 KEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQ 360
            EKMVCLQFAV+ARNKL PPLPKGFSGN+FVLASVAL A+QLE+ESYKTI+EMIR+AKS+
Sbjct: 301 NEKMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSR 360

Query: 361 VDDDYVNAYQLGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV 414
           VD+DY+NAY+ GL+GPQASLPPLKELTV+SDWTRMPFHKIEFLHG+A YASPLKPPILDV
Sbjct: 361 VDNDYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDV 420

BLAST of Carg02850 vs. ExPASy TrEMBL
Match: A0A1S3BW29 (omega-hydroxypalmitate O-feruloyl transferase OS=Cucumis melo OX=3656 GN=LOC103493765 PE=3 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 7.6e-173
Identity = 327/457 (71.55%), Postives = 354/457 (77.46%), Query Frame = 0

Query: 1   MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFT 60
           MA P VSLTKSL V PKT NP K LELSNLDRQCPLHMYLIFFYNPSSI TNSSLD VF 
Sbjct: 1   MANPQVSLTKSLIVIPKTSNPPKFLELSNLDRQCPLHMYLIFFYNPSSICTNSSLDLVFN 60

Query: 61  KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE 120
           KLKIGLEETLS+WSPAAGRL+ NP DGKLNLRCDNRGAIL +A+T+VKISELGDLSNYN+
Sbjct: 61  KLKIGLEETLSIWSPAAGRLDRNPTDGKLNLRCDNRGAILVQAITNVKISELGDLSNYND 120

Query: 121 FFEELVHKPVLDGDFCEFPLVVAQ---------------------------FLSAWATKS 180
           FFE+LV KPVL+G+F EFPLVVAQ                           FLSAWAT S
Sbjct: 121 FFEKLVQKPVLNGNFSEFPLVVAQVTRFGCGGYAIGTGISHSLFDGPAAYDFLSAWATNS 180

Query: 181 AIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLYKLIMQSA 240
           AIFKDD   Y ++   PVHERGRLL G+V NRCS          G VAIDHLYKLIMQSA
Sbjct: 181 AIFKDD--NYKMQTISPVHERGRLLTGTVANRCS----------GTVAIDHLYKLIMQSA 240

Query: 241 MGNEEMEKKEYGCRTLRVSRGMIETLKKKGFGE-----------------NVQARTKALS 300
           MGNEE+ +KEY CRT RVS+ MIE LK    GE                   +ARTKALS
Sbjct: 241 MGNEEVGRKEYVCRTFRVSKEMIERLKMNALGEINGGAFACSSFDLIAAHLWKARTKALS 300

Query: 301 VSKEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAK 360
           VSKEKMVCLQFAVDARNKL PPLPKGFSGN+FVLASVAL AKQL+EESYKTIIEMI+ AK
Sbjct: 301 VSKEKMVCLQFAVDARNKLMPPLPKGFSGNAFVLASVALTAKQLDEESYKTIIEMIQKAK 360

Query: 361 SQVDDDYVNAYQLGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPIL 414
           S+VD+DY+NAYQ GL+GPQASLPPLKELTV+SDWTRMPFHKI+FLHGEAAYASPLKPPIL
Sbjct: 361 SRVDNDYINAYQQGLEGPQASLPPLKELTVVSDWTRMPFHKIQFLHGEAAYASPLKPPIL 420

BLAST of Carg02850 vs. ExPASy TrEMBL
Match: A0A0A0L0F4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G652090 PE=3 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 9.9e-173
Identity = 323/457 (70.68%), Postives = 358/457 (78.34%), Query Frame = 0

Query: 1   MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFT 60
           MA P VSLTKSLTVFPKT NP+K +ELSNLDRQCPLHMYLIFFYNPSSIYTNSS D VF 
Sbjct: 1   MANPQVSLTKSLTVFPKTPNPTKFVELSNLDRQCPLHMYLIFFYNPSSIYTNSSPDLVFN 60

Query: 61  KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE 120
           KLKIGLEETLS+WSPAAGRL+ NP DGKLNLRCDNRGAIL +A+T+VK+SELGDLSNYNE
Sbjct: 61  KLKIGLEETLSIWSPAAGRLDRNPTDGKLNLRCDNRGAILVQAITNVKMSELGDLSNYNE 120

Query: 121 FFEELVHKPVLDGDFCEFPLVVAQ---------------------------FLSAWATKS 180
           FFE+LVHKP+L+G+F EFPLVVAQ                           FLSAWA  S
Sbjct: 121 FFEKLVHKPILNGNFSEFPLVVAQVTRFGCGGYAIGTGISHSLFDGPAAYDFLSAWAANS 180

Query: 181 AIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLYKLIMQSA 240
           AIFKD+ +   +    P+HERGRLL G+V NRCS       + A A AIDHLYKLIMQSA
Sbjct: 181 AIFKDNNKMQTI---TPIHERGRLLKGTVANRCS------RTVAVAAAIDHLYKLIMQSA 240

Query: 241 MGNEEMEKKEYGCRTLRVSRGMIETLKKKGFGEN-----------------VQARTKALS 300
           MGNEE+ +KEY CRT RVS+ MIE LK    GE                   +ARTKALS
Sbjct: 241 MGNEEVGRKEYVCRTFRVSKEMIERLKMNALGETNGAAFSCSSFDLIAAHLWKARTKALS 300

Query: 301 VSKEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAK 360
           VSKEKMVCLQFAVDARNKL PPLPKGFSGN+FVLASVAL AKQL+EESYKTIIEMI++AK
Sbjct: 301 VSKEKMVCLQFAVDARNKLMPPLPKGFSGNAFVLASVALTAKQLDEESYKTIIEMIQNAK 360

Query: 361 SQVDDDYVNAYQLGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPIL 414
           S+VD+DY+NAYQ GL+GPQASLPPLKELTV+SDWTRMPFHKI+FLHGEAAYASPLKPP+L
Sbjct: 361 SRVDNDYINAYQQGLEGPQASLPPLKELTVVSDWTRMPFHKIQFLHGEAAYASPLKPPVL 420

BLAST of Carg02850 vs. TAIR 10
Match: AT4G31910.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 324.3 bits (830), Expect = 1.5e-88
Identity = 201/466 (43.13%), Postives = 278/466 (59.66%), Query Frame = 0

Query: 1   MATPHVSLTKSLTVFPKTLN--PSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSV 60
           MAT  + + + L V+P+  N    KL+ LSNLDRQCPL MY +FFY  +   T    DSV
Sbjct: 5   MAT-RIDIIQKLNVYPRFQNHDKKKLITLSNLDRQCPLLMYSVFFYKNT---TTRDFDSV 64

Query: 61  FTKLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAV-TDVKISELGDLSN 120
           F+ LK+GLEET+SVW PAAGRL  +    KLN+RC++ GA++  AV T VK+SELGDL+ 
Sbjct: 65  FSNLKLGLEETMSVWYPAAGRLGLDGGGCKLNIRCNDGGAVMVEAVATGVKLSELGDLTQ 124

Query: 121 YNEFFEELVHKPVLDGDFCEFPLVVAQ---------------------------FLSAWA 180
           YNEF+E LV+KP LDGDF   PLVVAQ                           F+ AWA
Sbjct: 125 YNEFYENLVYKPSLDGDFSVMPLVVAQVTRFACGGYSIGIGTSHSLFDGISAYEFIHAWA 184

Query: 181 TKSAIFKDDKEQYGVELQ----KPVHERGRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLY 240
           + S I      +   + +    KPVH+R  LL+    NR + + +       A AI HLY
Sbjct: 185 SNSHIHNKSNSKITNKKEDVVIKPVHDRRNLLV----NRDAVRET------NAAAICHLY 244

Query: 241 KLIMQSAM------GNEEMEKKEYGCRTLRVSRGMIETLKKK---GF---------GENV 300
           +LI Q+ M       N E+    +  +T  ++   IE++KKK   GF             
Sbjct: 245 QLIKQAMMTYQEQNRNLELPDSGFVIKTFELNGDAIESMKKKSLEGFMCSSFEFLAAHLW 304

Query: 301 QARTKALSVSKEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQ-LEEESYKT 360
           +ART+AL + ++ MVCLQFAVD R + + PLP+GFSGN++VLASVA  A++ LEE + ++
Sbjct: 305 KARTRALGLRRDAMVCLQFAVDIRKRTETPLPEGFSGNAYVLASVASTARELLEELTLES 364

Query: 361 IIEMIRDAKSQVDDDYVNAYQLGLDGP-QASLPPLKELTVISDWTRMPFHKIEFLHG--E 411
           I+  IR+AK  +D  Y+N+Y   L G    +LPPLKELT+ISDWT+MPFH + F +G   
Sbjct: 365 IVNKIREAKKSIDQGYINSYMEALGGSNDGNLPPLKELTLISDWTKMPFHNVGFGNGGEP 424

BLAST of Carg02850 vs. TAIR 10
Match: AT5G41040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 104.8 bits (260), Expect = 1.9e-22
Identity = 108/425 (25.41%), Postives = 177/425 (41.65%), Query Frame = 0

Query: 14  VFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFTKLKIGLEETLSVW 73
           V P++     L  LSNLD+   + +  I+ +         ++  +    K  L + L  +
Sbjct: 41  VKPESETRKGLYFLSNLDQNIAVIVRTIYCFKSEERGNEEAVQVI----KKALSQVLVHY 100

Query: 74  SPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYN-EFFEELVHKPVLD 133
            P AGRL  +P +GKL + C   G +   A  + K+ E+GD++  + E   +LV+  V  
Sbjct: 101 YPLAGRLTISP-EGKLTVDCTEEGVVFVEAEANCKMDEIGDITKPDPETLGKLVYDVVDA 160

Query: 134 GDFCEFPLVVAQ--------FLSAWATKSAIFKD----------DKEQYGVELQKPVHER 193
            +  E P V AQ        F+        +F             +   G+ L  P    
Sbjct: 161 KNILEIPPVTAQVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNSWGQVARGLPLTTPPFSD 220

Query: 194 GRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLY---KLIMQSAMGNEEMEKK------EYG 253
             +L    P +                I+ LY     + +S   + E  KK      E  
Sbjct: 221 RTILNARNPPKIENLHQEFEEIEDKSNINSLYTKEPTLYRSFCFDPEKIKKLKLQATENS 280

Query: 254 CRTLRVSRGMIETLKKKGFGENVQARTKALSVSKEKMVCLQFAVDARNKLKPPLPKGFSG 313
              L  S    E L    +    +ARTK+L +  ++   L FAVD R K +P LPKG+ G
Sbjct: 281 ESLLGNSCTSFEALSAFVW----RARTKSLKMLSDQKTKLLFAVDGRAKFEPQLPKGYFG 340

Query: 314 NSFVLASVALPAKQLEEESYKTIIEMIRDAKSQVDDDYVNAYQLGLDGPQASLPPLKELT 373
           N  VL +    A +L E+     + ++R+A   V D Y+ +     +  +A  P L    
Sbjct: 341 NGIVLTNSICEAGELIEKPLSFAVGLVREAIKMVTDGYMRSAIDYFEVTRAR-PSLSSTL 400

Query: 374 VISDWTRMPFHKIEFLHGEAAYASPLKPPILDVAYFLQNPADSQAIDVRVTLLSQTLDAF 411
           +I+ W+R+ FH  +F  GE   + P+  P  +V  FL +    ++I+V + L +  +D F
Sbjct: 401 LITTWSRLGFHTTDFGWGEPILSGPVALPEKEVTLFLSHGEQRRSINVLLGLPATAMDVF 455

BLAST of Carg02850 vs. TAIR 10
Match: AT5G41040.2 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 104.8 bits (260), Expect = 1.9e-22
Identity = 108/425 (25.41%), Postives = 177/425 (41.65%), Query Frame = 0

Query: 14  VFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFTKLKIGLEETLSVW 73
           V P++     L  LSNLD+   + +  I+ +         ++  +    K  L + L  +
Sbjct: 25  VKPESETRKGLYFLSNLDQNIAVIVRTIYCFKSEERGNEEAVQVI----KKALSQVLVHY 84

Query: 74  SPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYN-EFFEELVHKPVLD 133
            P AGRL  +P +GKL + C   G +   A  + K+ E+GD++  + E   +LV+  V  
Sbjct: 85  YPLAGRLTISP-EGKLTVDCTEEGVVFVEAEANCKMDEIGDITKPDPETLGKLVYDVVDA 144

Query: 134 GDFCEFPLVVAQ--------FLSAWATKSAIFKD----------DKEQYGVELQKPVHER 193
            +  E P V AQ        F+        +F             +   G+ L  P    
Sbjct: 145 KNILEIPPVTAQVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNSWGQVARGLPLTTPPFSD 204

Query: 194 GRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLY---KLIMQSAMGNEEMEKK------EYG 253
             +L    P +                I+ LY     + +S   + E  KK      E  
Sbjct: 205 RTILNARNPPKIENLHQEFEEIEDKSNINSLYTKEPTLYRSFCFDPEKIKKLKLQATENS 264

Query: 254 CRTLRVSRGMIETLKKKGFGENVQARTKALSVSKEKMVCLQFAVDARNKLKPPLPKGFSG 313
              L  S    E L    +    +ARTK+L +  ++   L FAVD R K +P LPKG+ G
Sbjct: 265 ESLLGNSCTSFEALSAFVW----RARTKSLKMLSDQKTKLLFAVDGRAKFEPQLPKGYFG 324

Query: 314 NSFVLASVALPAKQLEEESYKTIIEMIRDAKSQVDDDYVNAYQLGLDGPQASLPPLKELT 373
           N  VL +    A +L E+     + ++R+A   V D Y+ +     +  +A  P L    
Sbjct: 325 NGIVLTNSICEAGELIEKPLSFAVGLVREAIKMVTDGYMRSAIDYFEVTRAR-PSLSSTL 384

Query: 374 VISDWTRMPFHKIEFLHGEAAYASPLKPPILDVAYFLQNPADSQAIDVRVTLLSQTLDAF 411
           +I+ W+R+ FH  +F  GE   + P+  P  +V  FL +    ++I+V + L +  +D F
Sbjct: 385 LITTWSRLGFHTTDFGWGEPILSGPVALPEKEVTLFLSHGEQRRSINVLLGLPATAMDVF 439

BLAST of Carg02850 vs. TAIR 10
Match: AT1G65450.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 100.9 bits (250), Expect = 2.7e-21
Identity = 106/439 (24.15%), Postives = 188/439 (42.82%), Query Frame = 0

Query: 6   VSLTKSLTVFP-KTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFTKLKI 65
           V++ +S  +FP +  +  K + LSN+D+     +  + F+ P+  +     + V  KL+ 
Sbjct: 18  VTIKESTLIFPSEETSERKSMFLSNVDQILNFDVQTVHFFRPNKEFPP---EMVSEKLRK 77

Query: 66  GLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNEFFEE 125
            L + +  +   AGRL  +P+ G+L++ C+  GA    A +D  + ELGDL   N  F +
Sbjct: 78  ALVKLMDAYEFLAGRLRVDPSSGRLDVDCNGAGAGFVTAASDYTLEELGDLVYPNPAFAQ 137

Query: 126 LVHKPVLDGDFCEFPLVVAQ--------FLSAWATKSAIFKDDKEQYGVE-LQKPVHERG 185
           LV   +      + PL V Q        F    +T    F     +  +E L   +HE+ 
Sbjct: 138 LVTSQLQSLPKDDQPLFVFQITSFKCGGFAMGISTNHTTFDGLSFKTFLENLASLLHEKP 197

Query: 186 RLLMGSVPNRCSGKRSAAGSGAGAVAIDHLYKLIMQSAMGNE--EMEKKEYGCRTLRVSR 245
              + + P  C+ +         +VA  H   +  Q        E   +    +  ++S 
Sbjct: 198 ---LSTPP--CNDRTLLKARDPPSVAFPHHELVKFQDCETTTVFEATSEHLDFKIFKLSS 257

Query: 246 GMIETLKKKG----------FGENVQA----RTKALSVSK--------EKMVCLQFAVDA 305
             I+ LK++            G NV      R KALSV+         E+   + +AVD 
Sbjct: 258 EQIKKLKERASETSNGNVRVTGFNVVTALVWRCKALSVAAEEGEETNLERESTILYAVDI 317

Query: 306 RNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQVDDDYVNAYQLGL 365
           R +L P LP  ++GN+ + A      K L EE +  I+EM+ +   ++ D+Y  +    +
Sbjct: 318 RGRLNPELPPSYTGNAVLTAYAKEKCKALLEEPFGRIVEMVGEGSKRITDEYARS---AI 377

Query: 366 DGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILD-VAYFLQNPADSQA 410
           D  +          ++S W ++ F ++E+  G+  Y+ P+     D V  F     DS+ 
Sbjct: 378 DWGELYKGFPHGEVLVSSWWKLGFAEVEYPWGKPKYSCPVVYHRKDIVLLFPDIDGDSKG 437

BLAST of Carg02850 vs. TAIR 10
Match: AT5G63560.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 96.7 bits (239), Expect = 5.1e-20
Identity = 105/436 (24.08%), Postives = 184/436 (42.20%), Query Frame = 0

Query: 6   VSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFTKLKIG 65
           V+  + + V P +  P  L  LSNLD+   + +   +++  +S     S +  +  +K  
Sbjct: 9   VTRKEPVLVSPASETPKGLHYLSNLDQNIAIIVKTFYYFKSNS----RSNEESYEVIKKS 68

Query: 66  LEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDV---KISELGDLSNYNEFF 125
           L E L  + PAAGRL  +P +GK+ + C   G ++  A  +    KI +     +  E  
Sbjct: 69  LSEVLVHYYPAAGRLTISP-EGKIAVDCTGEGVVVVEAEANCGIEKIKKAISEIDQPETL 128

Query: 126 EELVHKPVLDGDFCEFPLVVAQ---------------------------FLSAWATKSAI 185
           E+LV+      +  E P VV Q                           FL++WA  +  
Sbjct: 129 EKLVYDVPGARNILEIPPVVVQVTNFKCGGFVLGLGMNHNMFDGIAAMEFLNSWAETAR- 188

Query: 186 FKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKRSAAG-----SGAGAVAIDHLYKLIM 245
                   G+ L  P      LL    P +     +        SG G +  D   KL+ 
Sbjct: 189 --------GLPLSVPPFLDRTLLRPRTPPKIEFPHNEFEDLEDISGTGKLYSDE--KLVY 248

Query: 246 QSAM-GNEEMEKKEYGCRTLRVSRGMIETLKKKGFGENVQARTKALSVSKEKMVCLQFAV 305
           +S + G E++E+ +    T   +    +TL     G   +AR +AL +  ++ + L FA 
Sbjct: 249 KSFLFGPEKLERLKIMAETRSTT---FQTLT----GFLWRARCQALGLKPDQRIKLLFAA 308

Query: 306 DARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQVDDDYVNAYQL 365
           D R++  P LPKG+SGN  V       A ++        + +++ A   V+D ++ +   
Sbjct: 309 DGRSRFVPELPKGYSGNGIVFTYCVTTAGEVTLNPLSHSVCLVKRAVEMVNDGFMRSAID 368

Query: 366 GLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDVAYFLQNPADSQ 406
             +  +A  P L    +I+ W ++ FH  +F  GE   + P+  P  +V  FL   +D++
Sbjct: 369 YFEVTRAR-PSLTATLLITSWAKLSFHTKDFGWGEPVVSGPVGLPEKEVILFLPCGSDTK 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7024428.16.2e-254100.00Brassinosteroid-related acyltransferase 1, partial [Cucurbita argyrosperma subsp... [more]
KAG6591543.11.8e-22489.89Brassinosteroid-related acyltransferase 1, partial [Cucurbita argyrosperma subsp... [more]
XP_022936964.11.3e-22289.04brassinosteroid-related acyltransferase 1-like [Cucurbita moschata][more]
XP_023535946.16.3e-22288.79brassinosteroid-related acyltransferase 1-like [Cucurbita pepo subsp. pepo][more]
XP_022976161.12.3e-21687.47brassinosteroid-related acyltransferase 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SZ582.1e-8743.13Brassinosteroid-related acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=BAT... [more]
Q94CD12.6e-2125.41Omega-hydroxypalmitate O-feruloyl transferase OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9FFQ77.2e-1924.08Fatty alcohol:caffeoyl-CoA acyltransferase OS=Arabidopsis thaliana OX=3702 GN=FA... [more]
A0A2R6PLK21.7e-1521.70Alcohol acyltransferase 9 OS=Actinidia chinensis var. chinensis OX=1590841 GN=AT... [more]
A0A068BIF12.8e-1522.66Alcohol acyltransferase 9 OS=Actinidia eriantha OX=165200 GN=AT9 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F9T56.1e-22389.04brassinosteroid-related acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1IG591.1e-21687.47brassinosteroid-related acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1IT619.0e-17471.65brassinosteroid-related acyltransferase 1 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A1S3BW297.6e-17371.55omega-hydroxypalmitate O-feruloyl transferase OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A0A0L0F49.9e-17370.68Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G652090 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G31910.11.5e-8843.13HXXXD-type acyl-transferase family protein [more]
AT5G41040.11.9e-2225.41HXXXD-type acyl-transferase family protein [more]
AT5G41040.21.9e-2225.41HXXXD-type acyl-transferase family protein [more]
AT1G65450.12.7e-2124.15HXXXD-type acyl-transferase family protein [more]
AT5G63560.15.1e-2024.08HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003480TransferasePFAMPF02458Transferasecoord: 250..407
e-value: 9.2E-16
score: 57.5
coord: 5..144
e-value: 1.4E-18
score: 66.8
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 2..179
e-value: 2.2E-19
score: 71.8
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 207..410
e-value: 3.4E-33
score: 116.6
NoneNo IPR availablePANTHERPTHR31642TRICHOTHECENE 3-O-ACETYLTRANSFERASEcoord: 5..144
coord: 144..412
NoneNo IPR availablePANTHERPTHR31642:SF145BRASSINOSTEROID-RELATED ACYLTRANSFERASE 1coord: 144..412
coord: 5..144

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg02850-RACarg02850-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups