Carg02820 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg02820
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionAspartyl protease family protein
LocationCarg_Chr09: 1879296 .. 1881957 (-)
RNA-Seq ExpressionCarg02820
SyntenyCarg02820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGCCTCTTTCTTCAATGCTTTTCTTTGCTTTTTGTTTCCTTTTCTTTTACTCTTCCGCCGGGAAAGTCTCGCCGGACGCCCACCGCCTCACCGTCGAACTCGCTGACCTTCTGCCGTCCGCCGCCTGCAACCGCCGCTCCGTTCAAGGTCTCTCTCTGTTTCTCTAATTTCCTTCCGCTAGAATTTTCCGGCGTTATTAATTAAATTAAGATATATATTTTTTCTTAATTGGATTAGAAAATTTTATTTCGGTGAAATTAAATTTTATATAAGCACGAACTAACATTAATTTCGCTGTCAACTATATCATTACGTGTCCTAAACATAGGGAGGGCTGTTTCTTTCAACCTTAAATGTCTTGTTTTGTTGTTCGCCGATTTTTCTTATTCACCTTTTTAATTATAGATACGGGAAAATTAAAAGATTCCAAATTTTAATAATTTCACAAAAAAAAAAAAAAAAAAAGACCAATTGGGTCTGTCTGTCTGTCTGCCTCTAAGCCTTTTCTCCGTTGAAATTGAAGAAAATTGTATGGGTGGGGTTGTTGGTTTGTGGCTTAAAAGTAAAAGGGGAAATTATTTATTTATTTTTAATTAAAAAAAATCAAGAAAAGGGTCCAAATTTGTGATAAAGAAGTGACCGATCGAATTATTAACATGTTATTAAAAATACGACAAAAAAAAAATTAATTATAGAATTTTGTTTCCTTTTTCATAAGGGAATTGCTTTTTTTTTTTTTTTTTTTTTATGTTTTTAAAATTCTACTAACCTGCTAATAATTGTAATGTAATTCGAAAAAAGAATGTATATTTTTTAATTAAGGAGAAAATCTTTCTAGAAATCTGGCTAAATATTTTTAATGTTCTTAAATCATGGAAATAAATAATTAAAAATTGAAATGAAAACTGGTATTAGATGAGCTTGAGTTAGACGTCATCGTATTGAAACAAGTCAATGAATGATGGGGATAGAATCATTATTTTAATAAAATAAAAATTATTATATTTATAGTTAATTAGAATGGGGAAATTAATTCTTACGTAGAGAGTCAAAAGTAGGAAAAGACAAATAAGTACACCGTCAGTGAGTACAAATATTCCGCAACCCCATACGGCTGTGATGTAAAATGTAATTAAAGCGCCAGCTGAAGCCGCCGCTATAATTGCGAAACTATCAACTACTCAATGTGGGCCTCCAAGAGGCCCATTAATGAACAGCCCATTGGAAGCCCAAAATCGGAACCATATTTTACATTTTTTCTTCATTATTTATTTTTTAAAACAAGAAAATGTCATTTATGGGCCCAGCCCAAGATTCAGGTCGGAGAACGCAGTCATCACTGGAAGTGATTCACCGGCACGGGCCATGCGGCGGCGGAGAATCGAAAGCACCGACGGCAGAGGAATTATTCGTTCAAGATCAGGCTCGAGTGGATTTCATCCACTCGAAATTCTCAGGGGACTTCAAATCCATCGATCGTTTACGGCCGTCGAAGGCCACGAAAATCCCGGCGAAGTCCGGCGCCACCATCGGGTCCGGGAATTACATAGTGAATGTTGCTCTGGGGACGCCGAAGAAGTACTTATCGCTTATATTTGACACCGGCAGCGATCTGACGTGGACTCAGTGCGAGCCGTGTGCCAGATATTGCTACAGCCAGAAGGACCCTGTTTTCGCCCCTTCCCAATCCACCACTTATTCCAACATCACTTGTTCTTCGCCTATCTGCTCTCAGCTTGAATCGGGCACAGGTAATTTCACTTTAATTACTCATTTATAATAAATTTCATGTTGAATTTCAAGTTGAATTTCATGTTGTGTAAATATGCAGGGAACCAACCTGGTTGCTCCGCCGCGAAATCCTGCATTTACGGAATACAATACGGCGATCAATCTTTCTCCGTCGGATATTTCGCCAAAGAAACTTTAACCTTAACGCCCACCGATGTTATCAGCAATTTCCTCTTCGGTTGCGGCCAAAACAACCGTGGACTCTTCGGCAGCGCCGCCGGTCTCATTGGCCTTGGCCAGGACAAAATCTCCATCGTCAAACAGACCGCCCAAAAGTACGGTCAGATCTTCTCCTACTGCCTCCCTAAAACCTCAAGTTCCACCGGCTATCTAGCCTTCGGCGGCAGTGGCGGTGGCGGCGGTGCTCTGAAATACACGCCGATCACAAAAGCACACGGCGTCGCGAATTTCTATGGCGTCGACATCGTCGGTATGAAGGTCGGAGGAATTCAGATCCCGATCTCGGCGTCGGTCTTCTCGACCTCCGGCGCGATCATCGATTCCGGCACGGTGATTACGCGGCTGCCGCCAGATGCGTACAGCGCCTTGAAATCGGCGTTTCAGAAAGGAATGACGAAGTATCCGAAAGCGCCGGAGCTTTCGATTCTGGACACGTGTTACGATCTGAGCAAATACACCTCTGTAGAGATCCCGAAAGTGGATTTTCTTTTCAAAGGAGGAACGGTACTCGAACTGGACGGCACCGGAATCTTGTACGGAGCATCGACGACTCAGGTCTGTTTGGCATTCGCCGGAAATATGGATCCGAGCTCCGTTGCCATTATTGGGAACGTGCAGCAGAAGACTCTGCAGGTAGTTTACGATGTCGGTGGAGGGAAGATTGGGTTTGGTTATAATGGTTGTTAA

mRNA sequence

ATGGCGCCTCTTTCTTCAATGCTTTTCTTTGCTTTTTGTTTCCTTTTCTTTTACTCTTCCGCCGGGAAAGTCTCGCCGGACGCCCACCGCCTCACCGTCGAACTCGCTGACCTTCTGCCGTCCGCCGCCTGCAACCGCCGCTCCGTTCAAGCCCAAGATTCAGGTCGGAGAACGCAGTCATCACTGGAAGTGATTCACCGGCACGGGCCATGCGGCGGCGGAGAATCGAAAGCACCGACGGCAGAGGAATTATTCGTTCAAGATCAGGCTCGAGTGGATTTCATCCACTCGAAATTCTCAGGGGACTTCAAATCCATCGATCGTTTACGGCCGTCGAAGGCCACGAAAATCCCGGCGAAGTCCGGCGCCACCATCGGGTCCGGGAATTACATAGTGAATGTTGCTCTGGGGACGCCGAAGAAGTACTTATCGCTTATATTTGACACCGGCAGCGATCTGACGTGGACTCAGTGCGAGCCGTGTGCCAGATATTGCTACAGCCAGAAGGACCCTGTTTTCGCCCCTTCCCAATCCACCACTTATTCCAACATCACTTGTTCTTCGCCTATCTGCTCTCAGCTTGAATCGGGCACAGGGAACCAACCTGGTTGCTCCGCCGCGAAATCCTGCATTTACGGAATACAATACGGCGATCAATCTTTCTCCGTCGGATATTTCGCCAAAGAAACTTTAACCTTAACGCCCACCGATGTTATCAGCAATTTCCTCTTCGGTTGCGGCCAAAACAACCGTGGACTCTTCGGCAGCGCCGCCGGTCTCATTGGCCTTGGCCAGGACAAAATCTCCATCGTCAAACAGACCGCCCAAAAGTACGGTCAGATCTTCTCCTACTGCCTCCCTAAAACCTCAAGTTCCACCGGCTATCTAGCCTTCGGCGGCAGTGGCGGTGGCGGCGGTGCTCTGAAATACACGCCGATCACAAAAGCACACGGCGTCGCGAATTTCTATGGCGTCGACATCGTCGGTATGAAGGTCGGAGGAATTCAGATCCCGATCTCGGCGTCGGTCTTCTCGACCTCCGGCGCGATCATCGATTCCGGCACGGTGATTACGCGGCTGCCGCCAGATGCGTACAGCGCCTTGAAATCGGCGTTTCAGAAAGGAATGACGAAGTATCCGAAAGCGCCGGAGCTTTCGATTCTGGACACGTGTTACGATCTGAGCAAATACACCTCTGTAGAGATCCCGAAAGTGGATTTTCTTTTCAAAGGAGGAACGGTACTCGAACTGGACGGCACCGGAATCTTGTACGGAGCATCGACGACTCAGGTCTGTTTGGCATTCGCCGGAAATATGGATCCGAGCTCCGTTGCCATTATTGGGAACGTGCAGCAGAAGACTCTGCAGGTAGTTTACGATGTCGGTGGAGGGAAGATTGGGTTTGGTTATAATGGTTGTTAA

Coding sequence (CDS)

ATGGCGCCTCTTTCTTCAATGCTTTTCTTTGCTTTTTGTTTCCTTTTCTTTTACTCTTCCGCCGGGAAAGTCTCGCCGGACGCCCACCGCCTCACCGTCGAACTCGCTGACCTTCTGCCGTCCGCCGCCTGCAACCGCCGCTCCGTTCAAGCCCAAGATTCAGGTCGGAGAACGCAGTCATCACTGGAAGTGATTCACCGGCACGGGCCATGCGGCGGCGGAGAATCGAAAGCACCGACGGCAGAGGAATTATTCGTTCAAGATCAGGCTCGAGTGGATTTCATCCACTCGAAATTCTCAGGGGACTTCAAATCCATCGATCGTTTACGGCCGTCGAAGGCCACGAAAATCCCGGCGAAGTCCGGCGCCACCATCGGGTCCGGGAATTACATAGTGAATGTTGCTCTGGGGACGCCGAAGAAGTACTTATCGCTTATATTTGACACCGGCAGCGATCTGACGTGGACTCAGTGCGAGCCGTGTGCCAGATATTGCTACAGCCAGAAGGACCCTGTTTTCGCCCCTTCCCAATCCACCACTTATTCCAACATCACTTGTTCTTCGCCTATCTGCTCTCAGCTTGAATCGGGCACAGGGAACCAACCTGGTTGCTCCGCCGCGAAATCCTGCATTTACGGAATACAATACGGCGATCAATCTTTCTCCGTCGGATATTTCGCCAAAGAAACTTTAACCTTAACGCCCACCGATGTTATCAGCAATTTCCTCTTCGGTTGCGGCCAAAACAACCGTGGACTCTTCGGCAGCGCCGCCGGTCTCATTGGCCTTGGCCAGGACAAAATCTCCATCGTCAAACAGACCGCCCAAAAGTACGGTCAGATCTTCTCCTACTGCCTCCCTAAAACCTCAAGTTCCACCGGCTATCTAGCCTTCGGCGGCAGTGGCGGTGGCGGCGGTGCTCTGAAATACACGCCGATCACAAAAGCACACGGCGTCGCGAATTTCTATGGCGTCGACATCGTCGGTATGAAGGTCGGAGGAATTCAGATCCCGATCTCGGCGTCGGTCTTCTCGACCTCCGGCGCGATCATCGATTCCGGCACGGTGATTACGCGGCTGCCGCCAGATGCGTACAGCGCCTTGAAATCGGCGTTTCAGAAAGGAATGACGAAGTATCCGAAAGCGCCGGAGCTTTCGATTCTGGACACGTGTTACGATCTGAGCAAATACACCTCTGTAGAGATCCCGAAAGTGGATTTTCTTTTCAAAGGAGGAACGGTACTCGAACTGGACGGCACCGGAATCTTGTACGGAGCATCGACGACTCAGGTCTGTTTGGCATTCGCCGGAAATATGGATCCGAGCTCCGTTGCCATTATTGGGAACGTGCAGCAGAAGACTCTGCAGGTAGTTTACGATGTCGGTGGAGGGAAGATTGGGTTTGGTTATAATGGTTGTTAA

Protein sequence

MAPLSSMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQDSGRRTQSSLEVIHRHGPCGGGESKAPTAEELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAKSGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTTYSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVISNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGGSGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRLPPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGTGILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC
Homology
BLAST of Carg02820 vs. NCBI nr
Match: KAG7024399.1 (Aspartyl protease family protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 948.7 bits (2451), Expect = 1.9e-272
Identity = 473/473 (100.00%), Postives = 473/473 (100.00%), Query Frame = 0

Query: 1   MAPLSSMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQDSGRRTQS 60
           MAPLSSMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQDSGRRTQS
Sbjct: 1   MAPLSSMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQDSGRRTQS 60

Query: 61  SLEVIHRHGPCGGGESKAPTAEELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK 120
           SLEVIHRHGPCGGGESKAPTAEELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK
Sbjct: 61  SLEVIHRHGPCGGGESKAPTAEELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK 120

Query: 121 SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT 180
           SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT
Sbjct: 121 SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT 180

Query: 181 YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS 240
           YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS
Sbjct: 181 YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS 240

Query: 241 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG 300
           NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG
Sbjct: 241 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG 300

Query: 301 SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL 360
           SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL
Sbjct: 301 SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL 360

Query: 361 PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT 420
           PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT
Sbjct: 361 PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT 420

Query: 421 GILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
           GILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC
Sbjct: 421 GILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 473

BLAST of Carg02820 vs. NCBI nr
Match: XP_022936652.1 (aspartyl protease family protein At5g10770-like isoform X1 [Cucurbita moschata] >KAG6591512.1 Aspartyl protease family protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 946.4 bits (2445), Expect = 9.2e-272
Identity = 472/473 (99.79%), Postives = 472/473 (99.79%), Query Frame = 0

Query: 1   MAPLSSMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQDSGRRTQS 60
           MAPLSSMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQDSGRRTQS
Sbjct: 1   MAPLSSMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQDSGRRTQS 60

Query: 61  SLEVIHRHGPCGGGESKAPTAEELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK 120
           SLEVIHRHGPCGGGESKAPTA ELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK
Sbjct: 61  SLEVIHRHGPCGGGESKAPTAAELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK 120

Query: 121 SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT 180
           SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT
Sbjct: 121 SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT 180

Query: 181 YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS 240
           YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS
Sbjct: 181 YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS 240

Query: 241 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG 300
           NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG
Sbjct: 241 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG 300

Query: 301 SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL 360
           SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL
Sbjct: 301 SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL 360

Query: 361 PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT 420
           PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT
Sbjct: 361 PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT 420

Query: 421 GILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
           GILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC
Sbjct: 421 GILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 473

BLAST of Carg02820 vs. NCBI nr
Match: XP_023535774.1 (aspartyl protease family protein At5g10770-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 935.6 bits (2417), Expect = 1.6e-268
Identity = 465/473 (98.31%), Postives = 468/473 (98.94%), Query Frame = 0

Query: 1   MAPLSSMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQDSGRRTQS 60
           MAPLSSMLFFAFCFLFF+SSAGKVSP AHRLTVELADLLPSA CNRRSVQAQDSGRRTQS
Sbjct: 1   MAPLSSMLFFAFCFLFFHSSAGKVSPGAHRLTVELADLLPSATCNRRSVQAQDSGRRTQS 60

Query: 61  SLEVIHRHGPCGGGESKAPTAEELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK 120
           SLEVIHRHGPCGGGESKAPTA ELFVQDQ RVDFIHSKFSGDFKS+DRLRPSKATKIPAK
Sbjct: 61  SLEVIHRHGPCGGGESKAPTAAELFVQDQGRVDFIHSKFSGDFKSVDRLRPSKATKIPAK 120

Query: 121 SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT 180
           SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT
Sbjct: 121 SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT 180

Query: 181 YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS 240
           YSNITCSSPICSQLESGTGNQPGCS AKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS
Sbjct: 181 YSNITCSSPICSQLESGTGNQPGCSTAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS 240

Query: 241 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG 300
           NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG
Sbjct: 241 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG 300

Query: 301 SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL 360
           SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL
Sbjct: 301 SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL 360

Query: 361 PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT 420
           PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT
Sbjct: 361 PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT 420

Query: 421 GILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
           GILYGASTTQVCLAFAGNMDPS+VAIIGNVQQKTLQVVYDVGGGKIGFGYNGC
Sbjct: 421 GILYGASTTQVCLAFAGNMDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 473

BLAST of Carg02820 vs. NCBI nr
Match: XP_022936653.1 (aspartyl protease family protein At5g10770-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 933.3 bits (2411), Expect = 8.1e-268
Identity = 468/473 (98.94%), Postives = 468/473 (98.94%), Query Frame = 0

Query: 1   MAPLSSMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQDSGRRTQS 60
           MAPLSSMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQ    GRRTQS
Sbjct: 1   MAPLSSMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQ----GRRTQS 60

Query: 61  SLEVIHRHGPCGGGESKAPTAEELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK 120
           SLEVIHRHGPCGGGESKAPTA ELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK
Sbjct: 61  SLEVIHRHGPCGGGESKAPTAAELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK 120

Query: 121 SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT 180
           SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT
Sbjct: 121 SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT 180

Query: 181 YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS 240
           YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS
Sbjct: 181 YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS 240

Query: 241 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG 300
           NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG
Sbjct: 241 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG 300

Query: 301 SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL 360
           SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL
Sbjct: 301 SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL 360

Query: 361 PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT 420
           PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT
Sbjct: 361 PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT 420

Query: 421 GILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
           GILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC
Sbjct: 421 GILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 469

BLAST of Carg02820 vs. NCBI nr
Match: XP_022977010.1 (aspartyl protease family protein At5g10770-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 925.6 bits (2391), Expect = 1.7e-265
Identity = 462/473 (97.67%), Postives = 465/473 (98.31%), Query Frame = 0

Query: 1   MAPLSSMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQDSGRRTQS 60
           MAPLSSML FAFCF FF+SSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQ SG RTQS
Sbjct: 1   MAPLSSMLLFAFCFFFFHSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQISGGRTQS 60

Query: 61  SLEVIHRHGPCGGGESKAPTAEELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK 120
           SLEVIHRHGPCG G SKAPTA ELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK
Sbjct: 61  SLEVIHRHGPCGDGNSKAPTAAELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK 120

Query: 121 SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT 180
           SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQC PCARYCYSQKDPVFAPSQSTT
Sbjct: 121 SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCAPCARYCYSQKDPVFAPSQSTT 180

Query: 181 YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS 240
           YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS
Sbjct: 181 YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS 240

Query: 241 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG 300
           NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG
Sbjct: 241 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG 300

Query: 301 SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL 360
           SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL
Sbjct: 301 SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL 360

Query: 361 PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT 420
           PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT
Sbjct: 361 PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT 420

Query: 421 GILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
           GILYGASTTQVCLAFAGN+DPSSVAIIGNVQQKT+QVVYDVGGGKIGFGYNGC
Sbjct: 421 GILYGASTTQVCLAFAGNLDPSSVAIIGNVQQKTMQVVYDVGGGKIGFGYNGC 473

BLAST of Carg02820 vs. ExPASy Swiss-Prot
Match: Q8S9J6 (Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At5g10770 PE=2 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 6.2e-138
Identity = 253/450 (56.22%), Postives = 321/450 (71.33%), Query Frame = 0

Query: 27  DAHRLTVELADLLPSAACNRRSVQAQDSGRRTQSSLEVIHRHGPC---GGGESKAPTAEE 86
           D+H  T++++ LLPS++    S         T+SSL V HRHG C     G++ +P   E
Sbjct: 32  DSH--TIQVSSLLPSSS---SSCVLSPRASTTKSSLHVTHRHGTCSRLNNGKATSPDHVE 91

Query: 87  LFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAKSGATIGSGNYIVNVALGTPKKYL 146
           +   DQARV+ IHSK S    + D +  SK+T +PAK G+T+GSGNYIV V LGTPK  L
Sbjct: 92  ILRLDQARVNSIHSKLSKKL-ATDHVSESKSTDLPAKDGSTLGSGNYIVTVGLGTPKNDL 151

Query: 147 SLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTTYSNITCSSPICSQLESGTGNQPG 206
           SLIFDTGSDLTWTQC+PC R CY QK+P+F PS+ST+Y N++CSS  C  L S TGN   
Sbjct: 152 SLIFDTGSDLTWTQCQPCVRTCYDQKEPIFNPSKSTSYYNVSCSSAACGSLSSATGNAGS 211

Query: 207 CSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVISNFLFGCGQNNRGLFGSAAGLIGL 266
           CSA+ +CIYGIQYGDQSFSVG+ AKE  TLT +DV     FGCG+NN+GLF   AGL+GL
Sbjct: 212 CSAS-NCIYGIQYGDQSFSVGFLAKEKFTLTNSDVFDGVYFGCGENNQGLFTGVAGLLGL 271

Query: 267 GQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGGSGGGGGALKYTPITKAHGVANFY 326
           G+DK+S   QTA  Y +IFSYCLP ++S TG+L F GS G   ++K+TPI+      +FY
Sbjct: 272 GRDKLSFPSQTATAYNKIFSYCLPSSASYTGHLTF-GSAGISRSVKFTPISTITDGTSFY 331

Query: 327 GVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRLPPDAYSALKSAFQKGMTKYPKAP 386
           G++IV + VGG ++PI ++VFST GA+IDSGTVITRLPP AY+AL+S+F+  M+KYP   
Sbjct: 332 GLNIVAITVGGQKLPIPSTVFSTPGALIDSGTVITRLPPKAYAALRSSFKAKMSKYPTTS 391

Query: 387 ELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGTGILYGASTTQVCLAFAGNMDPSS 446
            +SILDTC+DLS + +V IPKV F F GG V+EL   GI Y    +QVCLAFAGN D S+
Sbjct: 392 GVSILDTCFDLSGFKTVTIPKVAFSFSGGAVVELGSKGIFYVFKISQVCLAFAGNSDDSN 451

Query: 447 VAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
            AI GNVQQ+TL+VVYD  GG++GF  NGC
Sbjct: 452 AAIFGNVQQQTLEVVYDGAGGRVGFAPNGC 473

BLAST of Carg02820 vs. ExPASy Swiss-Prot
Match: Q9LEW3 (Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 3.6e-117
Identity = 230/475 (48.42%), Postives = 306/475 (64.42%), Query Frame = 0

Query: 4   LSSMLFFAFCFLFFYSSAGKVSPDAHRL---TVELADLLPSAACNRRSVQAQDSGRRTQS 63
           LS ++    C  + ++   + S     L   T++++ L PS++    S         T+S
Sbjct: 8   LSMIIMLCVCLNWCFAEGAEKSDSGKVLDSYTIQVSSLFPSSS----SCVPSSKASNTKS 67

Query: 64  SLEVIHRHGPCGGGESKAPT-AEELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPA 123
           SL V+H HG C    S A    +E+  +DQARV+ I+SK S +  S + +  +K+T++PA
Sbjct: 68  SLRVVHMHGACSHLSSDARVDHDEIIRRDQARVESIYSKLSKN--SANEVSEAKSTELPA 127

Query: 124 KSGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQST 183
           KSG T+GSGNYIV + +GTPK  LSL+FDTGSDLTWTQCEPC   CYSQK+P F PS S+
Sbjct: 128 KSGITLGSGNYIVTIGIGTPKHDLSLVFDTGSDLTWTQCEPCLGSCYSQKEPKFNPSSSS 187

Query: 184 TYSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVI 243
           TY N++CSSP+C   ES       CSA+ +C+Y I YGD+SF+ G+ AKE  TLT +DV+
Sbjct: 188 TYQNVSCSSPMCEDAES-------CSAS-NCVYSIVYGDKSFTQGFLAKEKFTLTNSDVL 247

Query: 244 SNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPK-TSSSTGYLAF 303
            +  FGCG+NN+GLF   AGL+GLG  K+S+  QT   Y  IFSYCLP  TS+STG+L F
Sbjct: 248 EDVYFGCGENNQGLFDGVAGLLGLGPGKLSLPAQTTTTYNNIFSYCLPSFTSNSTGHLTF 307

Query: 304 GGSGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVIT 363
            GS G   ++K+TPI+      N YG+DI+G+ VG  ++ I+ + FST GAIIDSGTV T
Sbjct: 308 -GSAGISESVKFTPISSFPSAFN-YGIDIIGISVGDKELAITPNSFSTEGAIIDSGTVFT 367

Query: 364 RLPPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELD 423
           RLP   Y+ L+S F++ M+ Y       + DTCYD +   +V  P + F F G TV+ELD
Sbjct: 368 RLPTKVYAELRSVFKEKMSSYKSTSGYGLFDTCYDFTGLDTVTYPTIAFSFAGSTVVELD 427

Query: 424 GTGILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
           G+GI      +QVCLAFAGN D    AI GNVQQ TL VVYDV GG++GF  NGC
Sbjct: 428 GSGISLPIKISQVCLAFAGNDD--LPAIFGNVQQTTLDVVYDVAGGRVGFAPNGC 464

BLAST of Carg02820 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 2.0e-72
Identity = 152/362 (41.99%), Postives = 215/362 (59.39%), Query Frame = 0

Query: 121 SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT 180
           SG + GSG Y   + +GTP +Y+ ++ DTGSD+ W QC PC R CYSQ DP+F P +S T
Sbjct: 133 SGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRR-CYSQSDPIFDPRKSKT 192

Query: 181 YSNITCSSPICSQLESGTGNQPGCSA-AKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVI 240
           Y+ I CSSP C +L+S      GC+   K+C+Y + YGD SF+VG F+ ETLT    + +
Sbjct: 193 YATIPCSSPHCRRLDSA-----GCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFR-RNRV 252

Query: 241 SNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCL-PKTSSSTGYLAF 300
                GCG +N GLF  AAGL+GLG+ K+S   QT  ++ Q FSYCL  +++SS      
Sbjct: 253 KGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVV 312

Query: 301 GGSGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIP-ISASVF-----STSGAIID 360
            G+       ++TP+     +  FY V ++G+ VGG ++P ++AS+F        G IID
Sbjct: 313 FGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIID 372

Query: 361 SGTVITRLPPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGG 420
           SGT +TRL   AY A++ AF+ G     +AP+ S+ DTC+DLS    V++P V   F+G 
Sbjct: 373 SGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRGA 432

Query: 421 TVLELDGTGILYGASTT-QVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYN 474
            V  L  T  L    T  + C AFAG M    ++IIGN+QQ+  +VVYD+   ++GF   
Sbjct: 433 DV-SLPATNYLIPVDTNGKFCFAFAGTM--GGLSIIGNIQQQGFRVVYDLASSRVGFAPG 484

BLAST of Carg02820 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 1.8e-68
Identity = 144/360 (40.00%), Postives = 207/360 (57.50%), Query Frame = 0

Query: 121 SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT 180
           SG   GSG Y V + +G+P +   ++ D+GSD+ W QC+PC + CY Q DPVF P++S +
Sbjct: 122 SGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPC-KLCYKQSDPVFDPAKSGS 181

Query: 181 YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS 240
           Y+ ++C S +C ++E+      GC +   C Y + YGD S++ G  A ETLT   T V+ 
Sbjct: 182 YTGVSCGSSVCDRIENS-----GCHSG-GCRYEVMYGDGSYTKGTLALETLTFAKT-VVR 241

Query: 241 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCL-PKTSSSTGYLAFG 300
           N   GCG  NRG+F  AAGL+G+G   +S V Q + + G  F YCL  + + STG L FG
Sbjct: 242 NVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGSLVFG 301

Query: 301 GSGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTS-----GAIIDSG 360
                 GA  + P+ +     +FY V + G+ VGG++IP+   VF  +     G ++D+G
Sbjct: 302 REALPVGA-SWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTG 361

Query: 361 TVITRLPPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTV 420
           T +TRLP  AY A +  F+      P+A  +SI DTCYDLS + SV +P V F F  G V
Sbjct: 362 TAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPV 421

Query: 421 LELDGTGILYGASTT-QVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
           L L     L     +   C AFA +  P+ ++IIGN+QQ+ +QV +D   G +GFG N C
Sbjct: 422 LTLPARNFLMPVDDSGTYCFAFAAS--PTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of Carg02820 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 1.1e-65
Identity = 158/406 (38.92%), Postives = 221/406 (54.43%), Query Frame = 0

Query: 87  QDQARVDFIHSKFSGDFKSIDR--LRP---------SKATKIPAKSGATIGSGNYIVNVA 146
           +D +RV  I +K     + +DR  L+P         ++    P  SGA+ GSG Y   + 
Sbjct: 108 RDSSRVAGIVAKIRFAVEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIG 167

Query: 147 LGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTTYSNITCSSPICSQLE 206
           +GTP K + L+ DTGSD+ W QCEPCA  CY Q DPVF P+ S+TY ++TCS+P CS LE
Sbjct: 168 VGTPAKEMYLVLDTGSDVNWIQCEPCAD-CYQQSDPVFNPTSSSTYKSLTCSAPQCSLLE 227

Query: 207 SGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVISNFLFGCGQNNRGLFG 266
           +       C + K C+Y + YGD SF+VG  A +T+T   +  I+N   GCG +N GLF 
Sbjct: 228 TS-----ACRSNK-CLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFT 287

Query: 267 SAAGLIGLGQDKISIVKQTAQKYGQIFSYCL-PKTSSSTGYLAFGGSGGGGGALKYTPIT 326
            AAGL+GLG   +SI   T Q     FSYCL  + S  +  L F     GGG     P+ 
Sbjct: 288 GAAGLLGLGGGVLSI---TNQMKATSFSYCLVDRDSGKSSSLDFNSVQLGGGDAT-APLL 347

Query: 327 KAHGVANFYGVDIVGMKVGGIQIPISASVF-----STSGAIIDSGTVITRLPPDAYSALK 386
           +   +  FY V + G  VGG ++ +  ++F      + G I+D GT +TRL   AY++L+
Sbjct: 348 RNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLR 407

Query: 387 SAFQKGMTKYPK-APELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGTGILYGAST 446
            AF K      K +  +S+ DTCYD S  ++V++P V F F GG  L+L     L     
Sbjct: 408 DAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDD 467

Query: 447 T-QVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
           +   C AFA     SS++IIGNVQQ+  ++ YD+    IG   N C
Sbjct: 468 SGTFCFAFAPT--SSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of Carg02820 vs. ExPASy TrEMBL
Match: A0A6J1F908 (aspartyl protease family protein At5g10770-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443184 PE=3 SV=1)

HSP 1 Score: 946.4 bits (2445), Expect = 4.5e-272
Identity = 472/473 (99.79%), Postives = 472/473 (99.79%), Query Frame = 0

Query: 1   MAPLSSMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQDSGRRTQS 60
           MAPLSSMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQDSGRRTQS
Sbjct: 1   MAPLSSMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQDSGRRTQS 60

Query: 61  SLEVIHRHGPCGGGESKAPTAEELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK 120
           SLEVIHRHGPCGGGESKAPTA ELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK
Sbjct: 61  SLEVIHRHGPCGGGESKAPTAAELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK 120

Query: 121 SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT 180
           SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT
Sbjct: 121 SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT 180

Query: 181 YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS 240
           YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS
Sbjct: 181 YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS 240

Query: 241 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG 300
           NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG
Sbjct: 241 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG 300

Query: 301 SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL 360
           SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL
Sbjct: 301 SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL 360

Query: 361 PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT 420
           PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT
Sbjct: 361 PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT 420

Query: 421 GILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
           GILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC
Sbjct: 421 GILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 473

BLAST of Carg02820 vs. ExPASy TrEMBL
Match: A0A6J1FDU3 (aspartyl protease family protein At5g10770-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443184 PE=3 SV=1)

HSP 1 Score: 933.3 bits (2411), Expect = 3.9e-268
Identity = 468/473 (98.94%), Postives = 468/473 (98.94%), Query Frame = 0

Query: 1   MAPLSSMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQDSGRRTQS 60
           MAPLSSMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQ    GRRTQS
Sbjct: 1   MAPLSSMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQ----GRRTQS 60

Query: 61  SLEVIHRHGPCGGGESKAPTAEELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK 120
           SLEVIHRHGPCGGGESKAPTA ELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK
Sbjct: 61  SLEVIHRHGPCGGGESKAPTAAELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK 120

Query: 121 SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT 180
           SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT
Sbjct: 121 SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT 180

Query: 181 YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS 240
           YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS
Sbjct: 181 YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS 240

Query: 241 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG 300
           NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG
Sbjct: 241 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG 300

Query: 301 SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL 360
           SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL
Sbjct: 301 SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL 360

Query: 361 PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT 420
           PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT
Sbjct: 361 PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT 420

Query: 421 GILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
           GILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC
Sbjct: 421 GILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 469

BLAST of Carg02820 vs. ExPASy TrEMBL
Match: A0A6J1IL31 (aspartyl protease family protein At5g10770-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477209 PE=3 SV=1)

HSP 1 Score: 925.6 bits (2391), Expect = 8.2e-266
Identity = 462/473 (97.67%), Postives = 465/473 (98.31%), Query Frame = 0

Query: 1   MAPLSSMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQDSGRRTQS 60
           MAPLSSML FAFCF FF+SSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQ SG RTQS
Sbjct: 1   MAPLSSMLLFAFCFFFFHSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQISGGRTQS 60

Query: 61  SLEVIHRHGPCGGGESKAPTAEELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK 120
           SLEVIHRHGPCG G SKAPTA ELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK
Sbjct: 61  SLEVIHRHGPCGDGNSKAPTAAELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK 120

Query: 121 SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT 180
           SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQC PCARYCYSQKDPVFAPSQSTT
Sbjct: 121 SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCAPCARYCYSQKDPVFAPSQSTT 180

Query: 181 YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS 240
           YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS
Sbjct: 181 YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS 240

Query: 241 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG 300
           NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG
Sbjct: 241 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG 300

Query: 301 SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL 360
           SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL
Sbjct: 301 SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL 360

Query: 361 PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT 420
           PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT
Sbjct: 361 PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT 420

Query: 421 GILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
           GILYGASTTQVCLAFAGN+DPSSVAIIGNVQQKT+QVVYDVGGGKIGFGYNGC
Sbjct: 421 GILYGASTTQVCLAFAGNLDPSSVAIIGNVQQKTMQVVYDVGGGKIGFGYNGC 473

BLAST of Carg02820 vs. ExPASy TrEMBL
Match: A0A6J1III3 (aspartyl protease family protein At5g10770-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111477209 PE=3 SV=1)

HSP 1 Score: 916.0 bits (2366), Expect = 6.5e-263
Identity = 459/473 (97.04%), Postives = 462/473 (97.67%), Query Frame = 0

Query: 1   MAPLSSMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQDSGRRTQS 60
           MAPLSSML FAFCF FF+SSAGKVSPDAHRLTVELADLLPSAACNRRSVQ    G RTQS
Sbjct: 1   MAPLSSMLLFAFCFFFFHSSAGKVSPDAHRLTVELADLLPSAACNRRSVQ----GGRTQS 60

Query: 61  SLEVIHRHGPCGGGESKAPTAEELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK 120
           SLEVIHRHGPCG G SKAPTA ELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK
Sbjct: 61  SLEVIHRHGPCGDGNSKAPTAAELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAK 120

Query: 121 SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT 180
           SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQC PCARYCYSQKDPVFAPSQSTT
Sbjct: 121 SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCAPCARYCYSQKDPVFAPSQSTT 180

Query: 181 YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS 240
           YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS
Sbjct: 181 YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS 240

Query: 241 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG 300
           NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG
Sbjct: 241 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGG 300

Query: 301 SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL 360
           SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL
Sbjct: 301 SGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRL 360

Query: 361 PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT 420
           PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT
Sbjct: 361 PPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGT 420

Query: 421 GILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
           GILYGASTTQVCLAFAGN+DPSSVAIIGNVQQKT+QVVYDVGGGKIGFGYNGC
Sbjct: 421 GILYGASTTQVCLAFAGNLDPSSVAIIGNVQQKTMQVVYDVGGGKIGFGYNGC 469

BLAST of Carg02820 vs. ExPASy TrEMBL
Match: A0A1S3BVJ7 (aspartyl protease family protein At5g10770-like OS=Cucumis melo OX=3656 GN=LOC103493725 PE=4 SV=1)

HSP 1 Score: 814.3 bits (2102), Expect = 2.7e-232
Identity = 408/476 (85.71%), Postives = 436/476 (91.60%), Query Frame = 0

Query: 1   MAPLSSML--FFAFCFLFFYSSAGK-VSPDAHRLTVELADLLPSAACNRRSVQAQDSGRR 60
           MA L S++  FFAF FLFF S AGK +SPD+H LTVELADL PSA+C RRS QA  S   
Sbjct: 1   MASLPSIILFFFAFSFLFFQSFAGKLLSPDSHYLTVELADLFPSASCTRRSPQAHTSSVG 60

Query: 61  TQSSLEVIHRHGPCGGGESKAPTAEELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKI 120
            QSSLEVIHRHGPCG   S APTA E+FVQDQARVDFIHSKF+G+ +S+DRLRPSKATKI
Sbjct: 61  EQSSLEVIHRHGPCGDDVSNAPTAAEMFVQDQARVDFIHSKFAGELESVDRLRPSKATKI 120

Query: 121 PAKSGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQ 180
           PAKSGATIGSGNYIVNV LGTPKKYLSLIFDTGSDLTWTQC+PCARYCY+QKDPVFAPSQ
Sbjct: 121 PAKSGATIGSGNYIVNVGLGTPKKYLSLIFDTGSDLTWTQCQPCARYCYNQKDPVFAPSQ 180

Query: 181 STTYSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTD 240
           STTYSNI+CSS  CSQLESGTGNQPGCSAA++CIYGIQYGDQSFSVGYFAKETLTLT  D
Sbjct: 181 STTYSNISCSSSDCSQLESGTGNQPGCSAARACIYGIQYGDQSFSVGYFAKETLTLTSND 240

Query: 241 VISNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLA 300
           VI NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYL 
Sbjct: 241 VIENFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLT 300

Query: 301 FGGSGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVI 360
           FGG GGGGGALKYTPITKAHGVANFYG+DIVG+KVGG QIPIS+SVFSTSGAIIDSGTVI
Sbjct: 301 FGG-GGGGGALKYTPITKAHGVANFYGLDIVGIKVGGTQIPISSSVFSTSGAIIDSGTVI 360

Query: 361 TRLPPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLEL 420
           TRLPPDAYSALKSAFQKGM +YPKAPELSILDTCYDLSKY++++IPKV  +FKG   L+L
Sbjct: 361 TRLPPDAYSALKSAFQKGMARYPKAPELSILDTCYDLSKYSTIQIPKVGIMFKGQEELDL 420

Query: 421 DGTGILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
           DGTGI+YGAST+QVCLAFAGN DPS+VAIIGNVQQKTLQVVYDVGGGKIGFGYNGC
Sbjct: 421 DGTGIMYGASTSQVCLAFAGNQDPSTVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 475

BLAST of Carg02820 vs. TAIR 10
Match: AT5G10770.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 492.3 bits (1266), Expect = 4.4e-139
Identity = 253/450 (56.22%), Postives = 321/450 (71.33%), Query Frame = 0

Query: 27  DAHRLTVELADLLPSAACNRRSVQAQDSGRRTQSSLEVIHRHGPC---GGGESKAPTAEE 86
           D+H  T++++ LLPS++    S         T+SSL V HRHG C     G++ +P   E
Sbjct: 32  DSH--TIQVSSLLPSSS---SSCVLSPRASTTKSSLHVTHRHGTCSRLNNGKATSPDHVE 91

Query: 87  LFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPAKSGATIGSGNYIVNVALGTPKKYL 146
           +   DQARV+ IHSK S    + D +  SK+T +PAK G+T+GSGNYIV V LGTPK  L
Sbjct: 92  ILRLDQARVNSIHSKLSKKL-ATDHVSESKSTDLPAKDGSTLGSGNYIVTVGLGTPKNDL 151

Query: 147 SLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTTYSNITCSSPICSQLESGTGNQPG 206
           SLIFDTGSDLTWTQC+PC R CY QK+P+F PS+ST+Y N++CSS  C  L S TGN   
Sbjct: 152 SLIFDTGSDLTWTQCQPCVRTCYDQKEPIFNPSKSTSYYNVSCSSAACGSLSSATGNAGS 211

Query: 207 CSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVISNFLFGCGQNNRGLFGSAAGLIGL 266
           CSA+ +CIYGIQYGDQSFSVG+ AKE  TLT +DV     FGCG+NN+GLF   AGL+GL
Sbjct: 212 CSAS-NCIYGIQYGDQSFSVGFLAKEKFTLTNSDVFDGVYFGCGENNQGLFTGVAGLLGL 271

Query: 267 GQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGGSGGGGGALKYTPITKAHGVANFY 326
           G+DK+S   QTA  Y +IFSYCLP ++S TG+L F GS G   ++K+TPI+      +FY
Sbjct: 272 GRDKLSFPSQTATAYNKIFSYCLPSSASYTGHLTF-GSAGISRSVKFTPISTITDGTSFY 331

Query: 327 GVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRLPPDAYSALKSAFQKGMTKYPKAP 386
           G++IV + VGG ++PI ++VFST GA+IDSGTVITRLPP AY+AL+S+F+  M+KYP   
Sbjct: 332 GLNIVAITVGGQKLPIPSTVFSTPGALIDSGTVITRLPPKAYAALRSSFKAKMSKYPTTS 391

Query: 387 ELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGTGILYGASTTQVCLAFAGNMDPSS 446
            +SILDTC+DLS + +V IPKV F F GG V+EL   GI Y    +QVCLAFAGN D S+
Sbjct: 392 GVSILDTCFDLSGFKTVTIPKVAFSFSGGAVVELGSKGIFYVFKISQVCLAFAGNSDDSN 451

Query: 447 VAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
            AI GNVQQ+TL+VVYD  GG++GF  NGC
Sbjct: 452 AAIFGNVQQQTLEVVYDGAGGRVGFAPNGC 473

BLAST of Carg02820 vs. TAIR 10
Match: AT5G10760.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 423.3 bits (1087), Expect = 2.5e-118
Identity = 230/475 (48.42%), Postives = 306/475 (64.42%), Query Frame = 0

Query: 4   LSSMLFFAFCFLFFYSSAGKVSPDAHRL---TVELADLLPSAACNRRSVQAQDSGRRTQS 63
           LS ++    C  + ++   + S     L   T++++ L PS++    S         T+S
Sbjct: 8   LSMIIMLCVCLNWCFAEGAEKSDSGKVLDSYTIQVSSLFPSSS----SCVPSSKASNTKS 67

Query: 64  SLEVIHRHGPCGGGESKAPT-AEELFVQDQARVDFIHSKFSGDFKSIDRLRPSKATKIPA 123
           SL V+H HG C    S A    +E+  +DQARV+ I+SK S +  S + +  +K+T++PA
Sbjct: 68  SLRVVHMHGACSHLSSDARVDHDEIIRRDQARVESIYSKLSKN--SANEVSEAKSTELPA 127

Query: 124 KSGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQST 183
           KSG T+GSGNYIV + +GTPK  LSL+FDTGSDLTWTQCEPC   CYSQK+P F PS S+
Sbjct: 128 KSGITLGSGNYIVTIGIGTPKHDLSLVFDTGSDLTWTQCEPCLGSCYSQKEPKFNPSSSS 187

Query: 184 TYSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVI 243
           TY N++CSSP+C   ES       CSA+ +C+Y I YGD+SF+ G+ AKE  TLT +DV+
Sbjct: 188 TYQNVSCSSPMCEDAES-------CSAS-NCVYSIVYGDKSFTQGFLAKEKFTLTNSDVL 247

Query: 244 SNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPK-TSSSTGYLAF 303
            +  FGCG+NN+GLF   AGL+GLG  K+S+  QT   Y  IFSYCLP  TS+STG+L F
Sbjct: 248 EDVYFGCGENNQGLFDGVAGLLGLGPGKLSLPAQTTTTYNNIFSYCLPSFTSNSTGHLTF 307

Query: 304 GGSGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVIT 363
            GS G   ++K+TPI+      N YG+DI+G+ VG  ++ I+ + FST GAIIDSGTV T
Sbjct: 308 -GSAGISESVKFTPISSFPSAFN-YGIDIIGISVGDKELAITPNSFSTEGAIIDSGTVFT 367

Query: 364 RLPPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELD 423
           RLP   Y+ L+S F++ M+ Y       + DTCYD +   +V  P + F F G TV+ELD
Sbjct: 368 RLPTKVYAELRSVFKEKMSSYKSTSGYGLFDTCYDFTGLDTVTYPTIAFSFAGSTVVELD 427

Query: 424 GTGILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
           G+GI      +QVCLAFAGN D    AI GNVQQ TL VVYDV GG++GF  NGC
Sbjct: 428 GSGISLPIKISQVCLAFAGNDD--LPAIFGNVQQTTLDVVYDVAGGRVGFAPNGC 464

BLAST of Carg02820 vs. TAIR 10
Match: AT1G79720.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 287.3 bits (734), Expect = 2.2e-77
Identity = 170/444 (38.29%), Postives = 244/444 (54.95%), Query Frame = 0

Query: 41  SAACNRRSVQAQDSGRRTQSSLEVIHRHGPCGGGESKAPTAEELFVQDQARVDFIHSKFS 100
           S +C  RS+       R  ++LE+ HR    G             V D  RV  +  K  
Sbjct: 51  STSCFSRSL----GKGRESTTLEMKHRELCSGKTIDLGKKMRRALVLDNIRVQSLQLKIK 110

Query: 101 GDFKSIDRLRPSKATKIPAKSGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEP 160
               S      S+ T+IP  SG  + S NYIV V LG   K +SLI DTGSDLTW QC+P
Sbjct: 111 AMTSSTTEQSVSE-TQIPLTSGIKLESLNYIVTVELG--GKNMSLIVDTGSDLTWVQCQP 170

Query: 161 CARYCYSQKDPVFAPSQSTTYSNITCSSPICSQLESGTGNQPGCSAAKS-----CIYGIQ 220
           C R CY+Q+ P++ PS S++Y  + C+S  C  L + T N   C          C Y + 
Sbjct: 171 C-RSCYNQQGPLYDPSVSSSYKTVFCNSSTCQDLVAATSNSGPCGGNNGVVKTPCEYVVS 230

Query: 221 YGDQSFSVGYFAKETLTLTPTDVISNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTA 280
           YGD S++ G  A E++ L  T  + NF+FGCG+NN+GLFG ++GL+GLG+  +S+V QT 
Sbjct: 231 YGDGSYTRGDLASESILLGDTK-LENFVFGCGRNNKGLFGGSSGLMGLGRSSVSLVSQTL 290

Query: 281 QKYGQIFSYCLPK-TSSSTGYLAFGGSGG---GGGALKYTPITKAHGVANFYGVDIVGMK 340
           + +  +FSYCLP     ++G L+FG          ++ YTP+ +   + +FY +++ G  
Sbjct: 291 KTFNGVFSYCLPSLEDGASGSLSFGNDSSVYTNSTSVSYTPLVQNPQLRSFYILNLTGAS 350

Query: 341 VGGIQIPISASVFSTSGAIIDSGTVITRLPPDAYSALKSAFQKGMTKYPKAPELSILDTC 400
           +GG+++  S+      G +IDSGTVITRLPP  Y A+K  F K  + +P AP  SILDTC
Sbjct: 351 IGGVELKSSS---FGRGILIDSGTVITRLPPSIYKAVKIEFLKQFSGFPTAPGYSILDTC 410

Query: 401 YDLSKYTSVEIPKVDFLFKGGTVLELDGTGILY--GASTTQVCLAFAGNMDPSSVAIIGN 460
           ++L+ Y  + IP +  +F+G   LE+D TG+ Y      + VCLA A     + V IIGN
Sbjct: 411 FNLTSYEDISIPIIKMIFQGNAELEVDVTGVFYFVKPDASLVCLALASLSYENEVGIIGN 470

Query: 461 VQQKTLQVVYDVGGGKIGFGYNGC 474
            QQK  +V+YD    ++G     C
Sbjct: 471 YQQKNQRVIYDTTQERLGIVGENC 482

BLAST of Carg02820 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 274.6 bits (701), Expect = 1.5e-73
Identity = 152/362 (41.99%), Postives = 215/362 (59.39%), Query Frame = 0

Query: 121 SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT 180
           SG + GSG Y   + +GTP +Y+ ++ DTGSD+ W QC PC R CYSQ DP+F P +S T
Sbjct: 133 SGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRR-CYSQSDPIFDPRKSKT 192

Query: 181 YSNITCSSPICSQLESGTGNQPGCSA-AKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVI 240
           Y+ I CSSP C +L+S      GC+   K+C+Y + YGD SF+VG F+ ETLT    + +
Sbjct: 193 YATIPCSSPHCRRLDSA-----GCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFR-RNRV 252

Query: 241 SNFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCL-PKTSSSTGYLAF 300
                GCG +N GLF  AAGL+GLG+ K+S   QT  ++ Q FSYCL  +++SS      
Sbjct: 253 KGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVV 312

Query: 301 GGSGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIP-ISASVF-----STSGAIID 360
            G+       ++TP+     +  FY V ++G+ VGG ++P ++AS+F        G IID
Sbjct: 313 FGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIID 372

Query: 361 SGTVITRLPPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGG 420
           SGT +TRL   AY A++ AF+ G     +AP+ S+ DTC+DLS    V++P V   F+G 
Sbjct: 373 SGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRGA 432

Query: 421 TVLELDGTGILYGASTT-QVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYN 474
            V  L  T  L    T  + C AFAG M    ++IIGN+QQ+  +VVYD+   ++GF   
Sbjct: 433 DV-SLPATNYLIPVDTNGKFCFAFAGTM--GGLSIIGNIQQQGFRVVYDLASSRVGFAPG 484

BLAST of Carg02820 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 261.5 bits (667), Expect = 1.3e-69
Identity = 144/360 (40.00%), Postives = 207/360 (57.50%), Query Frame = 0

Query: 121 SGATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTT 180
           SG   GSG Y V + +G+P +   ++ D+GSD+ W QC+PC + CY Q DPVF P++S +
Sbjct: 122 SGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPC-KLCYKQSDPVFDPAKSGS 181

Query: 181 YSNITCSSPICSQLESGTGNQPGCSAAKSCIYGIQYGDQSFSVGYFAKETLTLTPTDVIS 240
           Y+ ++C S +C ++E+      GC +   C Y + YGD S++ G  A ETLT   T V+ 
Sbjct: 182 YTGVSCGSSVCDRIENS-----GCHSG-GCRYEVMYGDGSYTKGTLALETLTFAKT-VVR 241

Query: 241 NFLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCL-PKTSSSTGYLAFG 300
           N   GCG  NRG+F  AAGL+G+G   +S V Q + + G  F YCL  + + STG L FG
Sbjct: 242 NVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGSLVFG 301

Query: 301 GSGGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTS-----GAIIDSG 360
                 GA  + P+ +     +FY V + G+ VGG++IP+   VF  +     G ++D+G
Sbjct: 302 REALPVGA-SWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTG 361

Query: 361 TVITRLPPDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTV 420
           T +TRLP  AY A +  F+      P+A  +SI DTCYDLS + SV +P V F F  G V
Sbjct: 362 TAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPV 421

Query: 421 LELDGTGILYGASTT-QVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 474
           L L     L     +   C AFA +  P+ ++IIGN+QQ+ +QV +D   G +GFG N C
Sbjct: 422 LTLPARNFLMPVDDSGTYCFAFAAS--PTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7024399.11.9e-272100.00Aspartyl protease family protein, partial [Cucurbita argyrosperma subsp. argyros... [more]
XP_022936652.19.2e-27299.79aspartyl protease family protein At5g10770-like isoform X1 [Cucurbita moschata] ... [more]
XP_023535774.11.6e-26898.31aspartyl protease family protein At5g10770-like [Cucurbita pepo subsp. pepo][more]
XP_022936653.18.1e-26898.94aspartyl protease family protein At5g10770-like isoform X2 [Cucurbita moschata][more]
XP_022977010.11.7e-26597.67aspartyl protease family protein At5g10770-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8S9J66.2e-13856.22Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q9LEW33.6e-11748.42Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1[more]
Q9LNJ32.0e-7241.99Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q9LHE31.8e-6840.00Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LS401.1e-6538.92Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Match NameE-valueIdentityDescription
A0A6J1F9084.5e-27299.79aspartyl protease family protein At5g10770-like isoform X1 OS=Cucurbita moschata... [more]
A0A6J1FDU33.9e-26898.94aspartyl protease family protein At5g10770-like isoform X2 OS=Cucurbita moschata... [more]
A0A6J1IL318.2e-26697.67aspartyl protease family protein At5g10770-like isoform X1 OS=Cucurbita maxima O... [more]
A0A6J1III36.5e-26397.04aspartyl protease family protein At5g10770-like isoform X2 OS=Cucurbita maxima O... [more]
A0A1S3BVJ72.7e-23285.71aspartyl protease family protein At5g10770-like OS=Cucumis melo OX=3656 GN=LOC10... [more]
Match NameE-valueIdentityDescription
AT5G10770.14.4e-13956.22Eukaryotic aspartyl protease family protein [more]
AT5G10760.12.5e-11848.42Eukaryotic aspartyl protease family protein [more]
AT1G79720.12.2e-7738.29Eukaryotic aspartyl protease family protein [more]
AT1G01300.11.5e-7341.99Eukaryotic aspartyl protease family protein [more]
AT3G20015.11.3e-6940.00Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 136..156
score: 42.92
coord: 445..460
score: 30.21
coord: 349..360
score: 40.45
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 48..473
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 130..300
e-value: 8.3E-52
score: 176.0
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 305..473
e-value: 8.0E-44
score: 151.5
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 106..299
e-value: 8.1E-52
score: 178.0
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 124..473
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 323..468
e-value: 1.5E-29
score: 102.9
NoneNo IPR availablePANTHERPTHR13683:SF750ASPARTYL PROTEASE AED1coord: 48..473
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 145..156
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 130..469
score: 43.062088
IPR033873CND41-likeCDDcd05472cnd41_likecoord: 129..473
e-value: 4.65827E-136
score: 392.02

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg02820-RACarg02820-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity