Carg02757 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg02757
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	(1->3)-beta-glucan endohydrolase
Location	Carg_Chr02: 7017000 .. 7019326 (+)
RNA-Seq Expression	Carg02757
Synteny	Carg02757

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CGTAGCGCACCGTCCTCCGACTGGTCGGCAGTTCCCCTCTTCCTATACTGGTTTTGAAGGTACTGGTTGATACTTCATTGCCGATTTTCTTAATATTCATACATTTTTATTGTCATTTTCAGTAGATTTTTTACTCGAGCTGGGAATGGCGGGGATTGGAGGTGTTATTGTGAACTGCTGCGATTTCTGTTCCTGTTGCATCGAGGTTCATGATGCTTGTTGTAATAGTAGTTTGCTGCCTTTCCCATGCCATGGAGCGGCAGATATTGGGGGTTCTCTGTTTATTTGTGGGGATTAAGGTTTTTATCGTTTTATTTCATCAGGGTTTTCTTCAGTTGTAGGAAATTCGATCTCGTTGGTTGGGTCGTTTGTTGTTCTGTATTCTACTCTCATTTGTGCTTATGGGTTAATGTTTGGTAATTAGTGGTTAGCTACTTGCTTACATATGAATTTGTTCGTTGTTTGTATCTGCTTGTTTTGGTTTAGGGTCTCTTCCTGTTATAATTCTGTGTAAGAAGTGATTGTGATCTTGATAGGAATGTTATTAGGATAGAATTAGGGATGTTTTTGGGGTATTAAAGGTATGTTAGTAATTAGGTAATGAGTTGCTTGGGATAGAATTATTTAGATGTTTTTGACTTGGACTGAGAGTATGTTAGTAATCTTTTGTATTTCAATATCATTTTGGTTCTGTTTGGTTCGATCTTGTACTCGTTCTTTTCCATATGCAACGAGCACGACTCTGATGTACTGTCGATATCTTGCAGTCTTTATTGAATTTTGTTCTCTCTTGGTGGCATTTCATCGTAATTTTGACAGGCCGGTAAACTAAACTGCTGAGGGACGACTCGAAGGAACGGAGAGATGTTTGAGGTTGACGTTTTGGTGAATTGGAGGAGGCATGAGAAATGGTCTGCCATTGTGCTTTTGCTTGTGTCTGCCATGTTTTTTCATGTAAGAGGTACAGTATCTTAAATGCTAAAATTCAATTCAAGAGCGACCTCCTTCTGTAACACTCACAGCATGCATTCTTCCTTCTGTGTTACCCAGCATTTGTTGGGATAAATATTGGGACTCATGTATCCAATCTTCCACCTGCTTCAAAGGTGGTTTCAATGCTCAAACTCTACAAAATAACTCATGTTCGACTCTACGATGCCGATGTGCAATTACTCAAAGCCTTGGCAAACAGTAGCATTGAAGTAATGGTTGGTGTTACAAATGAGGAGGTTCTAAAACTTGGAAAGTCTCCATCAGCGGCTGCAGCTTGGATTAACAAAAATGTTGCACTTTATATACCTGCAACCAATATAACAGCCATTGCTGTAGGCAGTGAGGTTCTTACGTCTTTCCCCAATGCAGGCCCGTTCTTGATCCGTGCCATGAAATACCTTCATAAAGCTCTTGTGGCAGCGAACCTAAATCTTCAAGTCAAAGTTTCAACTCCTCAGTCTATGGATGTCGTTGTTACGTCGTTTCCTCCTTCCACAGCCACATTCAATTCTTCATTGAATTCCACCATTTACCCACTCCTTCAGTTTTTGAAGAACACCAAGTCATATTATATGTTGAATGTTTATCCTTATTATGGATACATGAAAGGAAACGGGATTTTCCCACTAGATTATGCTCTTTTCCGGCCGTTGTCTATTAACCGGCGGATTGTTGACCCGAACACTCTCCTCCACTATACCAACATGTTTGATGCCATGTTGGATGCAACCTATCATTCAATAAAATCTCTCAACTTTTCTGGTATCCCCATTATGGTGACTGAATCTGGCTGGCCTTGGTCTGGTGGAGCCAATGAAACTTATGCAACCAAGAAAAATGCTGAGATATATGTGAACAATCTGATCGGTAGGGTTTTGAATGGCTCTGGTCCACCTAGCGAGCCAACTAATCCTGTTAACGCCTACGTATATGAGTTGTTCAGTGAAGACCAGAAGCCTGGGCCTATGTCAGAGAAAAACTGGGGAGTTCTTTCGACCAACGGTTCTGCTATATATCGTTTGAGTTTGATATCCAGCAGTGTCACTGGGAGTTCTTCTGTTGTTTACTGTGTGGCTAAAGATGGTGCAGATCCTGGTAAGTTGCAAAGTGGTCTGAACTGGGCTTGTGGGCAGGGTGGAGCCAATTGTTCTGCTATTCAACCAGGGCAACCGTGTTTTCTTCCCGATAACGTTGTAAACCATGCTTCTTATGCTTATAATGACTACTATCAGAAAATGCAAGGCAATGGCGGAACATGTGACTTTGAAGGCACAGCAACATTAACTAATACTAATCCAAGTAAGTTCCTTTCTCCAAGTTCTTGCTTTATCTAA

mRNA sequence

CGTAGCGCACCGTCCTCCGACTGGTCGGCAGTTCCCCTCTTCCTATACTGGTTTTGAAGGCCGGTAAACTAAACTGCTGAGGGACGACTCGAAGGAACGGAGAGATGTTTGAGGTTGACGTTTTGGTGAATTGGAGGAGGCATGAGAAATGGTCTGCCATTGTGCTTTTGCTTGTGTCTGCCATGTTTTTTCATGTAAGAGCATTTGTTGGGATAAATATTGGGACTCATGTATCCAATCTTCCACCTGCTTCAAAGGTGGTTTCAATGCTCAAACTCTACAAAATAACTCATGTTCGACTCTACGATGCCGATGTGCAATTACTCAAAGCCTTGGCAAACAGTAGCATTGAAGTAATGGTTGGTGTTACAAATGAGGAGGTTCTAAAACTTGGAAAGTCTCCATCAGCGGCTGCAGCTTGGATTAACAAAAATGTTGCACTTTATATACCTGCAACCAATATAACAGCCATTGCTGTAGGCAGTGAGGTTCTTACGTCTTTCCCCAATGCAGGCCCGTTCTTGATCCGTGCCATGAAATACCTTCATAAAGCTCTTGTGGCAGCGAACCTAAATCTTCAAGTCAAAGTTTCAACTCCTCAGTCTATGGATGTCGTTGTTACGTCGTTTCCTCCTTCCACAGCCACATTCAATTCTTCATTGAATTCCACCATTTACCCACTCCTTCAGTTTTTGAAGAACACCAAGTCATATTATATGTTGAATGTTTATCCTTATTATGGATACATGAAAGGAAACGGGATTTTCCCACTAGATTATGCTCTTTTCCGGCCGTTGTCTATTAACCGGCGGATTGTTGACCCGAACACTCTCCTCCACTATACCAACATGTTTGATGCCATGTTGGATGCAACCTATCATTCAATAAAATCTCTCAACTTTTCTGGTATCCCCATTATGGTGACTGAATCTGGCTGGCCTTGGTCTGGTGGAGCCAATGAAACTTATGCAACCAAGAAAAATGCTGAGATATATGTGAACAATCTGATCGGTAGGGTTTTGAATGGCTCTGGTCCACCTAGCGAGCCAACTAATCCTGTTAACGCCTACGTATATGAGTTGTTCAGTGAAGACCAGAAGCCTGGGCCTATGTCAGAGAAAAACTGGGGAGTTCTTTCGACCAACGGTTCTGCTATATATCGTTTGAGTTTGATATCCAGCAGTGTCACTGGGAGTTCTTCTGTTGTTTACTGTGTGGCTAAAGATGGTGCAGATCCTGGTAAGTTGCAAAGTGGTCTGAACTGGGCTTGTGGGCAGGGTGGAGCCAATTGTTCTGCTATTCAACCAGGGCAACCGTGTTTTCTTCCCGATAACGTTGTAAACCATGCTTCTTATGCTTATAATGACTACTATCAGAAAATGCAAGGCAATGGCGGAACATGTGACTTTGAAGGCACAGCAACATTAACTAATACTAATCCAAGTAAGTTCCTTTCTCCAAGTTCTTGCTTTATCTAA

Coding sequence (CDS)

ATGTTTGAGGTTGACGTTTTGGTGAATTGGAGGAGGCATGAGAAATGGTCTGCCATTGTGCTTTTGCTTGTGTCTGCCATGTTTTTTCATGTAAGAGCATTTGTTGGGATAAATATTGGGACTCATGTATCCAATCTTCCACCTGCTTCAAAGGTGGTTTCAATGCTCAAACTCTACAAAATAACTCATGTTCGACTCTACGATGCCGATGTGCAATTACTCAAAGCCTTGGCAAACAGTAGCATTGAAGTAATGGTTGGTGTTACAAATGAGGAGGTTCTAAAACTTGGAAAGTCTCCATCAGCGGCTGCAGCTTGGATTAACAAAAATGTTGCACTTTATATACCTGCAACCAATATAACAGCCATTGCTGTAGGCAGTGAGGTTCTTACGTCTTTCCCCAATGCAGGCCCGTTCTTGATCCGTGCCATGAAATACCTTCATAAAGCTCTTGTGGCAGCGAACCTAAATCTTCAAGTCAAAGTTTCAACTCCTCAGTCTATGGATGTCGTTGTTACGTCGTTTCCTCCTTCCACAGCCACATTCAATTCTTCATTGAATTCCACCATTTACCCACTCCTTCAGTTTTTGAAGAACACCAAGTCATATTATATGTTGAATGTTTATCCTTATTATGGATACATGAAAGGAAACGGGATTTTCCCACTAGATTATGCTCTTTTCCGGCCGTTGTCTATTAACCGGCGGATTGTTGACCCGAACACTCTCCTCCACTATACCAACATGTTTGATGCCATGTTGGATGCAACCTATCATTCAATAAAATCTCTCAACTTTTCTGGTATCCCCATTATGGTGACTGAATCTGGCTGGCCTTGGTCTGGTGGAGCCAATGAAACTTATGCAACCAAGAAAAATGCTGAGATATATGTGAACAATCTGATCGGTAGGGTTTTGAATGGCTCTGGTCCACCTAGCGAGCCAACTAATCCTGTTAACGCCTACGTATATGAGTTGTTCAGTGAAGACCAGAAGCCTGGGCCTATGTCAGAGAAAAACTGGGGAGTTCTTTCGACCAACGGTTCTGCTATATATCGTTTGAGTTTGATATCCAGCAGTGTCACTGGGAGTTCTTCTGTTGTTTACTGTGTGGCTAAAGATGGTGCAGATCCTGGTAAGTTGCAAAGTGGTCTGAACTGGGCTTGTGGGCAGGGTGGAGCCAATTGTTCTGCTATTCAACCAGGGCAACCGTGTTTTCTTCCCGATAACGTTGTAAACCATGCTTCTTATGCTTATAATGACTACTATCAGAAAATGCAAGGCAATGGCGGAACATGTGACTTTGAAGGCACAGCAACATTAACTAATACTAATCCAAGTAAGTTCCTTTCTCCAAGTTCTTGCTTTATCTAA

Protein sequence

MFEVDVLVNWRRHEKWSAIVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSMLKLYKITHVRLYDADVQLLKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNITAIAVGSEVLTSFPNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTATFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDPNTLLHYTNMFDAMLDATYHSIKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNNLIGRVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSEKNWGVLSTNGSAIYRLSLISSSVTGSSSVVYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYNDYYQKMQGNGGTCDFEGTATLTNTNPSKFLSPSSCFI

Homology

[Close]

BLAST of Carg02757 vs. NCBI nr
Match: KAG7035815.1 (Glucan endo-1,3-beta-glucosidase 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 914.8 bits (2363), Expect = 2.9e-262
Identity = 457/457 (100.00%), Postives = 457/457 (100.00%), Query Frame = 0


Query: 1   MFEVDVLVNWRRHEKWSAIVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSMLKLYK 60
           MFEVDVLVNWRRHEKWSAIVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSMLKLYK
Sbjct: 1   MFEVDVLVNWRRHEKWSAIVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSMLKLYK 60

Query: 61  ITHVRLYDADVQLLKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNI 120
           ITHVRLYDADVQLLKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNI
Sbjct: 61  ITHVRLYDADVQLLKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNI 120

Query: 121 TAIAVGSEVLTSFPNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTA 180
           TAIAVGSEVLTSFPNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTA
Sbjct: 121 TAIAVGSEVLTSFPNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTA 180

Query: 181 TFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDP 240
           TFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDP
Sbjct: 181 TFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDP 240

Query: 241 NTLLHYTNMFDAMLDATYHSIKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNN 300
           NTLLHYTNMFDAMLDATYHSIKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNN
Sbjct: 241 NTLLHYTNMFDAMLDATYHSIKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNN 300

Query: 301 LIGRVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSEKNWGVLSTNGSAIYRLSLISSS 360
           LIGRVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSEKNWGVLSTNGSAIYRLSLISSS
Sbjct: 301 LIGRVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSEKNWGVLSTNGSAIYRLSLISSS 360

Query: 361 VTGSSSVVYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYN 420
           VTGSSSVVYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYN
Sbjct: 361 VTGSSSVVYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYN 420

Query: 421 DYYQKMQGNGGTCDFEGTATLTNTNPSKFLSPSSCFI 458
           DYYQKMQGNGGTCDFEGTATLTNTNPSKFLSPSSCFI
Sbjct: 421 DYYQKMQGNGGTCDFEGTATLTNTNPSKFLSPSSCFI 457

[Close]

BLAST of Carg02757 vs. NCBI nr
Match: KAG6605865.1 (Glucan endo-1,3-beta-glucosidase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 892.5 bits (2305), Expect = 1.5e-255
Identity = 446/447 (99.78%), Postives = 446/447 (99.78%), Query Frame = 0


Query: 1   MFEVDVLVNWRRHEKWSAIVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSMLKLYK 60
           MFEVDVLVNWRRHEKWSAIVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSMLKLYK
Sbjct: 1   MFEVDVLVNWRRHEKWSAIVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSMLKLYK 60

Query: 61  ITHVRLYDADVQLLKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNI 120
           ITHVRLYDAD QLLKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNI
Sbjct: 61  ITHVRLYDADAQLLKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNI 120

Query: 121 TAIAVGSEVLTSFPNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTA 180
           TAIAVGSEVLTSFPNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTA
Sbjct: 121 TAIAVGSEVLTSFPNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTA 180

Query: 181 TFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDP 240
           TFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDP
Sbjct: 181 TFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDP 240

Query: 241 NTLLHYTNMFDAMLDATYHSIKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNN 300
           NTLLHYTNMFDAMLDATYHSIKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNN
Sbjct: 241 NTLLHYTNMFDAMLDATYHSIKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNN 300

Query: 301 LIGRVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSEKNWGVLSTNGSAIYRLSLISSS 360
           LIGRVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSEKNWGVLSTNGSAIYRLSLISSS
Sbjct: 301 LIGRVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSEKNWGVLSTNGSAIYRLSLISSS 360

Query: 361 VTGSSSVVYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYN 420
           VTGSSSVVYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYN
Sbjct: 361 VTGSSSVVYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYN 420

Query: 421 DYYQKMQGNGGTCDFEGTATLTNTNPS 448
           DYYQKMQGNGGTCDFEGTATLTNTNPS
Sbjct: 421 DYYQKMQGNGGTCDFEGTATLTNTNPS 447

[Close]

BLAST of Carg02757 vs. NCBI nr
Match: XP_022957736.1 (glucan endo-1,3-beta-glucosidase 4-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 885.6 bits (2287), Expect = 1.9e-253
Identity = 440/447 (98.43%), Postives = 445/447 (99.55%), Query Frame = 0


Query: 1   MFEVDVLVNWRRHEKWSAIVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSMLKLYK 60
           MFEVDVLVNWRRHEKWSAIVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSML+LYK
Sbjct: 1   MFEVDVLVNWRRHEKWSAIVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSMLELYK 60

Query: 61  ITHVRLYDADVQLLKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNI 120
           ITHVRLYDAD QLLKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNI
Sbjct: 61  ITHVRLYDADAQLLKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNI 120

Query: 121 TAIAVGSEVLTSFPNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTA 180
           TAIAVGSEVLTSFPNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTA
Sbjct: 121 TAIAVGSEVLTSFPNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTA 180

Query: 181 TFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDP 240
           TFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDP
Sbjct: 181 TFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDP 240

Query: 241 NTLLHYTNMFDAMLDATYHSIKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNN 300
           NTLLHYTNMFDAMLDATYHS+KSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNN
Sbjct: 241 NTLLHYTNMFDAMLDATYHSVKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNN 300

Query: 301 LIGRVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSEKNWGVLSTNGSAIYRLSLISSS 360
           L+GRVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSE NWGVLSTNGSAIYRLSLISSS
Sbjct: 301 LLGRVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSENNWGVLSTNGSAIYRLSLISSS 360

Query: 361 VTGSSSVVYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYN 420
           VTGSSS+VYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYN
Sbjct: 361 VTGSSSIVYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYN 420

Query: 421 DYYQKMQGNGGTCDFEGTATLTNTNPS 448
           DYYQKMQG+GGTCDFEGTATLTNTNPS
Sbjct: 421 DYYQKMQGHGGTCDFEGTATLTNTNPS 447

[Close]

BLAST of Carg02757 vs. NCBI nr
Match: XP_023533890.1 (glucan endo-1,3-beta-glucosidase 4-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 881.7 bits (2277), Expect = 2.7e-252
Identity = 440/447 (98.43%), Postives = 443/447 (99.11%), Query Frame = 0


Query: 1   MFEVDVLVNWRRHEKWSAIVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSMLKLYK 60
           MFEVDVLVNWRRHEKWSAIVLLLVSAMFFHVRAFVGINIGTHVSNLPPAS VVSMLKLYK
Sbjct: 1   MFEVDVLVNWRRHEKWSAIVLLLVSAMFFHVRAFVGINIGTHVSNLPPASNVVSMLKLYK 60

Query: 61  ITHVRLYDADVQLLKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNI 120
           ITHVRLYDAD QLLKALANSSIEVMVGVTNEEVLK+GKSPS AAAWINKNVALYIPATNI
Sbjct: 61  ITHVRLYDADGQLLKALANSSIEVMVGVTNEEVLKIGKSPSVAAAWINKNVALYIPATNI 120

Query: 121 TAIAVGSEVLTSFPNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTA 180
           TAIAVGSEVLTSFPNAGPFLIR MKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTA
Sbjct: 121 TAIAVGSEVLTSFPNAGPFLIRVMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTA 180

Query: 181 TFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDP 240
           TFNSSLNSTIYPLLQFLK+TKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDP
Sbjct: 181 TFNSSLNSTIYPLLQFLKDTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDP 240

Query: 241 NTLLHYTNMFDAMLDATYHSIKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNN 300
           NTLLHYTNMFDAMLDATYHSIKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNN
Sbjct: 241 NTLLHYTNMFDAMLDATYHSIKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNN 300

Query: 301 LIGRVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSEKNWGVLSTNGSAIYRLSLISSS 360
           LIGRVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSEKNWGVLST+GSAIYRLSLISSS
Sbjct: 301 LIGRVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSEKNWGVLSTDGSAIYRLSLISSS 360

Query: 361 VTGSSSVVYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYN 420
           VTGSSSVVYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYN
Sbjct: 361 VTGSSSVVYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYN 420

Query: 421 DYYQKMQGNGGTCDFEGTATLTNTNPS 448
           DYYQKMQGNGGTCDFEGTATLTNTNPS
Sbjct: 421 DYYQKMQGNGGTCDFEGTATLTNTNPS 447

[Close]

BLAST of Carg02757 vs. NCBI nr
Match: XP_022996336.1 (glucan endo-1,3-beta-glucosidase 4-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 865.9 bits (2236), Expect = 1.5e-247
Identity = 434/447 (97.09%), Postives = 436/447 (97.54%), Query Frame = 0


Query: 1   MFEVDVLVNWRRHEKWSAIVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSMLKLYK 60
           MFEVDVLVNWRRHEKW AIVLLLVSAMFFHVRAFVGINIGTHVSNLPPAS VVSMLKLYK
Sbjct: 1   MFEVDVLVNWRRHEKWFAIVLLLVSAMFFHVRAFVGINIGTHVSNLPPASNVVSMLKLYK 60

Query: 61  ITHVRLYDADVQLLKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNI 120
           ITHVRLYDAD QLLKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPA NI
Sbjct: 61  ITHVRLYDADAQLLKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPAINI 120

Query: 121 TAIAVGSEVLTSFPNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTA 180
           TAIAVGSEVLTS PNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVV+TSFPPSTA
Sbjct: 121 TAIAVGSEVLTSIPNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVLTSFPPSTA 180

Query: 181 TFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDP 240
           TFNSSLNSTIYPLLQFLK TKSYYMLNVYPYYGYMKGNGIFPLDYAL RPLSINRRIVDP
Sbjct: 181 TFNSSLNSTIYPLLQFLKTTKSYYMLNVYPYYGYMKGNGIFPLDYALLRPLSINRRIVDP 240

Query: 241 NTLLHYTNMFDAMLDATYHSIKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNN 300
           NTLLHYTNMFDAMLDATYHSIKSLNFS IPI+VTESGWPWSGGANETYATKKNAEIYVNN
Sbjct: 241 NTLLHYTNMFDAMLDATYHSIKSLNFSDIPIVVTESGWPWSGGANETYATKKNAEIYVNN 300

Query: 301 LIGRVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSEKNWGVLSTNGSAIYRLSLISSS 360
           LI RVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSEKNWGVL TNGSAIYRLSLISSS
Sbjct: 301 LISRVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSEKNWGVLLTNGSAIYRLSLISSS 360

Query: 361 VTGSSSVVYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYN 420
           VTGSSSVVYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQ CFLPDNVVNHASYAYN
Sbjct: 361 VTGSSSVVYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQTCFLPDNVVNHASYAYN 420

Query: 421 DYYQKMQGNGGTCDFEGTATLTNTNPS 448
           DYYQKMQGNGGTCDFEGTATLTNTNPS
Sbjct: 421 DYYQKMQGNGGTCDFEGTATLTNTNPS 447

[Close]

BLAST of Carg02757 vs. ExPASy Swiss-Prot
Match: Q94CD8 (Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 PE=2 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 1.4e-158
Identity = 274/436 (62.84%), Postives = 351/436 (80.50%), Query Frame = 0


Query: 15  KWSA-IVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSMLKLYKITHVRLYDADVQL 74
           +W A  +LLL+S +     AF+G+NIGT ++N+PP S +V++LK  +ITHVRLYDA+  +
Sbjct: 5   RWFAEALLLLLSILACSNAAFIGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHM 64

Query: 75  LKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNITAIAVGSEVLTSF 134
           LKA AN+SIEVMVGVTNEE+LK+G+ PSAAAAW+NKNVA YIP+TNITAIAVGSEVLT+ 
Sbjct: 65  LKAFANTSIEVMVGVTNEEILKIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTI 124

Query: 135 PNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTATFNSSLNSTIYPL 194
           P+  P L  A+  +HKALVA+NLN +VKVS+P SMD++   FPPST+TF+ S N+T+Y L
Sbjct: 125 PHVAPILASALNNIHKALVASNLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSWNTTVYQL 184

Query: 195 LQFLKNTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDPNTLLHYTNMFDAM 254
           LQFLKNT S++MLN YPYYGY   NGIFPLDYALF+ LS  ++IVDPNTLLHY +MFDAM
Sbjct: 185 LQFLKNTGSFFMLNAYPYYGYTTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAM 244

Query: 255 LDATYHSIKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNNLIGRVLNGSGPPS 314
           +DA Y+S+++LNFS IP++VTE+GWP SGG++E  AT  NAE +  NLI RVLN SGPPS
Sbjct: 245 VDAAYYSMEALNFSKIPVVVTETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPS 304

Query: 315 EPTNPVNAYVYELFSEDQKPGPMSEKNWGVLSTNGSAIYRLSLI--SSSVTGSSSVVYCV 374
           +P  P+N Y+YEL++ED++ GP+SE+NWG+L  NG+++Y LSL   SSS   + S ++CV
Sbjct: 305 QPDIPINTYIYELYNEDKRSGPVSERNWGILFPNGTSVYPLSLSGGSSSAALNGSSMFCV 364

Query: 375 AKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYNDYYQKMQGNGG 434
           AK  AD  KL  GLNWACGQG ANC+AIQPGQPC+LP++V +HAS+A+NDYYQKM+  GG
Sbjct: 365 AKADADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPNDVKSHASFAFNDYYQKMKSAGG 424

Query: 435 TCDFEGTATLTNTNPS 448
           TCDF+GTA  T  +PS
Sbjct: 425 TCDFDGTAITTTRDPS 440

[Close]

BLAST of Carg02757 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 443.4 bits (1139), Expect = 3.2e-123
Identity = 215/433 (49.65%), Postives = 304/433 (70.21%), Query Frame = 0


Query: 19  IVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSMLKLYKITHVRLYDADVQLLKALA 78
           + L L ++      + +G+NIGT V+N+P  ++VV++LK   I  VRLYDAD  +L A A
Sbjct: 7   LFLFLFASSALSQDSLIGVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADRSMLLAFA 66

Query: 79  NSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNITAIAVGSEVLTSFPNAGP 138
           ++ ++V++ V N+++L + +S + AA W+ +NVA Y PATNIT IAVGSEVLTS  NA  
Sbjct: 67  HTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSLTNAAS 126

Query: 139 FLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTATFNSSLNSTIYPLLQFLK 198
            L+ A+KY+  ALV ANL+ Q+KVSTP S  +++ SFPPS A FN + +  I PLL+FL+
Sbjct: 127 VLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFNKTWDPVIVPLLKFLQ 186

Query: 199 NTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDPNTLLHYTNMFDAMLDATY 258
           +T S  +LNVYPY+ Y++ NG+ PLDYALF+PL  N+  VD NTLLHYTN+FDA++DA Y
Sbjct: 187 STGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDAIVDAAY 246

Query: 259 HSIKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNNLIGRVLNGSGPPSEPTNP 318
            ++  LNF+ IPI+VTESGWP  GG +E  AT +NA  Y +NLI  V+N +G P  P   
Sbjct: 247 FAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTPKHPGTA 306

Query: 319 VNAYVYELFSEDQKPGPMSEKNWGVLSTNGSAIYRLSLISSS---VTGSSSVVYCVAKDG 378
           V  Y+YEL++ED +PGP+SEKNWG+  TNG+ +Y L L  +       +++  +C+AK+ 
Sbjct: 307 VTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTLRLAGAGAILANDTTNQTFCIAKEK 366

Query: 379 ADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYNDYYQKMQGNGGTCDF 438
            D   LQ+ L+WACG G  +CSA+  G+ C+ PD+VV H++YA+N YYQKM    G+CDF
Sbjct: 367 VDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFNAYYQKMGKASGSCDF 426

Query: 439 EGTATLTNTNPSK 449
           +G AT+T T+PS+
Sbjct: 427 KGVATVTTTDPSR 439

[Close]

BLAST of Carg02757 vs. ExPASy Swiss-Prot
Match: Q9C7U5 (Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 PE=2 SV=2)

HSP 1 Score: 427.6 bits (1098), Expect = 1.8e-118
Identity = 210/418 (50.24%), Postives = 296/418 (70.81%), Query Frame = 0


Query: 33  AFVGINIGTHVSNLPPASKVVSMLKLYKITHVRLYDADVQLLKALANSSIEVMVGVTNEE 92
           +++G+NIGT +S++P  ++VV++LK  +I H+RLY+AD  LL ALAN+ I+V++ + N++
Sbjct: 30  SYIGVNIGTDLSDMPHPTQVVALLKAQEIRHIRLYNADPGLLIALANTGIKVIISIPNDQ 89

Query: 93  VLKLGKSPSAAAAWINKNVALYIPATNITAIAVGSEVLTSFPNAGPFLIRAMKYLHKALV 152
           +L +G+S S AA W+ +NV  + PAT ITA++VGSEVLTS  NA P L+ A+K +H AL+
Sbjct: 90  LLGIGQSNSTAANWVKRNVIAHYPATMITAVSVGSEVLTSLSNAAPVLVSAIKNVHAALL 149

Query: 153 AANLNLQVKVSTPQSMDVVVTSFPPSTATFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYY 212
           +ANL+  +KVSTP S  +++  FPPS A FN SLN+ I PLL FL++T SY M+NVYPY 
Sbjct: 150 SANLDKLIKVSTPLSTSLILDPFPPSQAFFNRSLNAVIVPLLSFLQSTNSYLMVNVYPYI 209

Query: 213 GYMKGNGIFPLDYALFRPLSINRRIVDPNTLLHYTNMFDAMLDATYHSIKSLNFSGIPIM 272
            YM+ NG+ PLDYALF+P+  N+  VD NTL+ Y+N FDAM+DATY ++  LNF+ IP++
Sbjct: 210 DYMQSNGVIPLDYALFKPIPPNKEAVDANTLVRYSNAFDAMVDATYFAMAFLNFTNIPVL 269

Query: 273 VTESGWPWSGGANETYATKKNAEIYVNNLIGRVLNGSGPPSEPTNPVNAYVYELFSEDQK 332
           VTESGWP  G  NE  AT  NA  Y +NLI  VLN +G P  P   V+ Y+YEL++ED K
Sbjct: 270 VTESGWPSKGETNEPDATLDNANTYNSNLIRHVLNKTGTPKRPGIAVSTYIYELYNEDTK 329

Query: 333 PGPMSEKNWGVLSTNGSAIYRLSLISSS---VTGSSSVVYCVAKDGADPGKLQSGLNWAC 392
            G +SEKNWG+ + NG  +Y L L +S       +++  YC A++GAD   LQ+ L+WAC
Sbjct: 330 AG-LSEKNWGLFNANGEPVYVLRLTNSGSVLANDTTNQTYCTAREGADTKMLQAALDWAC 389

Query: 393 GQGGANCSAIQPGQPCFLPDNVVNHASYAYNDYYQKMQGNGGTCDFEGTATLTNTNPS 448
           G G  +CS I+ G+ C+ PDNVV HA+YA++ YY +   N   C+F G A++T T+PS
Sbjct: 390 GPGKIDCSPIKQGETCYEPDNVVAHANYAFDTYYHQTGNNPDACNFNGVASITTTDPS 446

[Close]

BLAST of Carg02757 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 419.9 bits (1078), Expect = 3.8e-116
Identity = 207/417 (49.64%), Postives = 287/417 (68.82%), Query Frame = 0


Query: 34  FVGINIGTHVSNLPPASKVVSMLKLYKITHVRLYDADVQLLKALANSSIEVMVGVTNEEV 93
           FVG NIGT VSNL   +++V  L+  K+ HVRLYDAD +LLKALA + + V++ V N ++
Sbjct: 43  FVGFNIGTDVSNLLSPTELVKFLQAQKVNHVRLYDADPELLKALAKTKVRVIISVPNNQL 102

Query: 94  LKLGKSPSAAAAWINKNVALYIPATNITAIAVGSEVLTSFPNAGPFLIRAMKYLHKALVA 153
           L +G S S AA+WI +NV  Y P T ITAI+VG EVLT+ P++ P L+ A++ L+ ALVA
Sbjct: 103 LAIGSSNSTAASWIGRNVVAYYPETLITAISVGDEVLTTVPSSAPLLLPAIESLYNALVA 162

Query: 154 ANLNLQVKVSTPQSMDVVVTSFPPSTATFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYYG 213
           +NL+ Q+KVSTP +  +++ +FPPS A FN + +S + PLLQFL  T S  M+N+YPYY 
Sbjct: 163 SNLHTQIKVSTPHAASIMLDTFPPSQAYFNQTWHSIMVPLLQFLSKTGSPLMMNLYPYYV 222

Query: 214 YMKGNGIFPLDYALFRPLSINRRIVDPNTLLHYTNMFDAMLDATYHSIKSLNFSGIPIMV 273
           YM+  G+ PLD  LF PL+ ++ +VDPNTLLHYTN+ DAM+DA Y S+K+LN S + ++V
Sbjct: 223 YMQNKGVVPLDNCLFEPLTPSKEMVDPNTLLHYTNVLDAMVDAAYVSMKNLNVSDVAVLV 282

Query: 274 TESGWPWSGGANETYATKKNAEIYVNNLIGRVLNGSGPPSEPTNPVNAYVYELFSEDQKP 333
           TESGWP  G + E YAT  NA+ Y +NLI  V + +G P  P    + Y+YELF+ED + 
Sbjct: 283 TESGWPSKGDSKEPYATIDNADTYNSNLIKHVFDRTGTPLHPEMTSSVYIYELFNEDLRA 342

Query: 334 GPMSEKNWGVLSTNGSAIYRLSLISSS---VTGSSSVVYCVAKDGADPGKLQSGLNWACG 393
            P+SE +WG+   N + +Y L +  S       +++  YC+A DG D   LQ+ L+WACG
Sbjct: 343 PPVSEASWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTYCIAMDGVDAKTLQAALDWACG 402

Query: 394 QGGANCSAIQPGQPCFLPDNVVNHASYAYNDYYQKMQGNGGTCDFEGTATLTNTNPS 448
            G +NCS IQPG+ C+ P+NV  HAS+A+N YYQK     G+CDF+G A +T T+PS
Sbjct: 403 PGRSNCSEIQPGESCYQPNNVKGHASFAFNSYYQKEGRASGSCDFKGVAMITTTDPS 459

[Close]

BLAST of Carg02757 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 4.4e-88
Identity = 172/462 (37.23%), Postives = 273/462 (59.09%), Query Frame = 0


Query: 19  IVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSMLKLYKITHVRLYDADVQLLKALA 78
           I + +++ + F + + +GI  G +  NLP  ++V  +++   I  VR+YDA++ +LKA A
Sbjct: 11  IFVSILAFLNFGMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFA 70

Query: 79  NSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNITAIAVGSEVLTSFPNAGP 138
           N+ IE+M+GV N ++L   +  S    W++ N+  Y P+T IT+I+VG EV  +  NA  
Sbjct: 71  NTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNATG 130

Query: 139 FLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTATFNSSLNSTIYPLLQFLK 198
            ++ AM+ +H AL  + L+ ++K+S+  S+ ++  SFPPS+A+F+   ++ + P+L+FL 
Sbjct: 131 LVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSAFLKPMLEFLV 190

Query: 199 NTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDPNTLLHYTNMFDAMLDATY 258
             +S +M+++YPYY Y       PL+YALF   S   ++VDP T L Y+NMFDA LDA Y
Sbjct: 191 ENESPFMIDLYPYYAYRDSTEKVPLEYALFESSS---QVVDPATGLLYSNMFDAQLDAIY 250

Query: 259 HSIKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNNLIGRVLNGSGPPSEPTNP 318
            ++ +++F  + +MVTESGWP  G   ET AT +NA  Y  NLI  V+   G P++P   
Sbjct: 251 FALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEE 310

Query: 319 VNAYVYELFSEDQKPGPMSEKNWGVLSTNGSAIYRLSLISSSVT---------------- 378
           ++ Y++ LF+E++KPG  SE+NWG+   NG+ +Y L     + T                
Sbjct: 311 IDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPS 370

Query: 379 ------GSSSVV-----------YCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQPC 438
                 G+S+V            +C+A   A   +LQ+ L+WACG G  +CSA+QP QPC
Sbjct: 371 SSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPC 430

Query: 439 FLPDNVVNHASYAYNDYYQKMQGNGGTCDFEGTATLTNTNPS 448
           F PD V++HASYA+N YYQ+   +   C F G +   + +PS
Sbjct: 431 FEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPS 469

[Close]

BLAST of Carg02757 vs. ExPASy TrEMBL
Match: A0A6J1H028 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111459192 PE=3 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 9.0e-254
Identity = 440/447 (98.43%), Postives = 445/447 (99.55%), Query Frame = 0


Query: 1   MFEVDVLVNWRRHEKWSAIVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSMLKLYK 60
           MFEVDVLVNWRRHEKWSAIVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSML+LYK
Sbjct: 1   MFEVDVLVNWRRHEKWSAIVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSMLELYK 60

Query: 61  ITHVRLYDADVQLLKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNI 120
           ITHVRLYDAD QLLKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNI
Sbjct: 61  ITHVRLYDADAQLLKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNI 120

Query: 121 TAIAVGSEVLTSFPNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTA 180
           TAIAVGSEVLTSFPNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTA
Sbjct: 121 TAIAVGSEVLTSFPNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTA 180

Query: 181 TFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDP 240
           TFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDP
Sbjct: 181 TFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDP 240

Query: 241 NTLLHYTNMFDAMLDATYHSIKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNN 300
           NTLLHYTNMFDAMLDATYHS+KSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNN
Sbjct: 241 NTLLHYTNMFDAMLDATYHSVKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNN 300

Query: 301 LIGRVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSEKNWGVLSTNGSAIYRLSLISSS 360
           L+GRVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSE NWGVLSTNGSAIYRLSLISSS
Sbjct: 301 LLGRVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSENNWGVLSTNGSAIYRLSLISSS 360

Query: 361 VTGSSSVVYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYN 420
           VTGSSS+VYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYN
Sbjct: 361 VTGSSSIVYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYN 420

Query: 421 DYYQKMQGNGGTCDFEGTATLTNTNPS 448
           DYYQKMQG+GGTCDFEGTATLTNTNPS
Sbjct: 421 DYYQKMQGHGGTCDFEGTATLTNTNPS 447

[Close]

BLAST of Carg02757 vs. ExPASy TrEMBL
Match: A0A6J1K4F9 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491597 PE=3 SV=1)

HSP 1 Score: 865.9 bits (2236), Expect = 7.4e-248
Identity = 434/447 (97.09%), Postives = 436/447 (97.54%), Query Frame = 0


Query: 1   MFEVDVLVNWRRHEKWSAIVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSMLKLYK 60
           MFEVDVLVNWRRHEKW AIVLLLVSAMFFHVRAFVGINIGTHVSNLPPAS VVSMLKLYK
Sbjct: 1   MFEVDVLVNWRRHEKWFAIVLLLVSAMFFHVRAFVGINIGTHVSNLPPASNVVSMLKLYK 60

Query: 61  ITHVRLYDADVQLLKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNI 120
           ITHVRLYDAD QLLKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPA NI
Sbjct: 61  ITHVRLYDADAQLLKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPAINI 120

Query: 121 TAIAVGSEVLTSFPNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTA 180
           TAIAVGSEVLTS PNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVV+TSFPPSTA
Sbjct: 121 TAIAVGSEVLTSIPNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVLTSFPPSTA 180

Query: 181 TFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDP 240
           TFNSSLNSTIYPLLQFLK TKSYYMLNVYPYYGYMKGNGIFPLDYAL RPLSINRRIVDP
Sbjct: 181 TFNSSLNSTIYPLLQFLKTTKSYYMLNVYPYYGYMKGNGIFPLDYALLRPLSINRRIVDP 240

Query: 241 NTLLHYTNMFDAMLDATYHSIKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNN 300
           NTLLHYTNMFDAMLDATYHSIKSLNFS IPI+VTESGWPWSGGANETYATKKNAEIYVNN
Sbjct: 241 NTLLHYTNMFDAMLDATYHSIKSLNFSDIPIVVTESGWPWSGGANETYATKKNAEIYVNN 300

Query: 301 LIGRVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSEKNWGVLSTNGSAIYRLSLISSS 360
           LI RVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSEKNWGVL TNGSAIYRLSLISSS
Sbjct: 301 LISRVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSEKNWGVLLTNGSAIYRLSLISSS 360

Query: 361 VTGSSSVVYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYN 420
           VTGSSSVVYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQ CFLPDNVVNHASYAYN
Sbjct: 361 VTGSSSVVYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQTCFLPDNVVNHASYAYN 420

Query: 421 DYYQKMQGNGGTCDFEGTATLTNTNPS 448
           DYYQKMQGNGGTCDFEGTATLTNTNPS
Sbjct: 421 DYYQKMQGNGGTCDFEGTATLTNTNPS 447

[Close]

BLAST of Carg02757 vs. ExPASy TrEMBL
Match: A0A6J1GZY9 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111459192 PE=3 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 3.6e-234
Identity = 407/415 (98.07%), Postives = 413/415 (99.52%), Query Frame = 0


Query: 33  AFVGINIGTHVSNLPPASKVVSMLKLYKITHVRLYDADVQLLKALANSSIEVMVGVTNEE 92
           +FVGINIGTHVSNLPPASKVVSML+LYKITHVRLYDAD QLLKALANSSIEVMVGVTNEE
Sbjct: 16  SFVGINIGTHVSNLPPASKVVSMLELYKITHVRLYDADAQLLKALANSSIEVMVGVTNEE 75

Query: 93  VLKLGKSPSAAAAWINKNVALYIPATNITAIAVGSEVLTSFPNAGPFLIRAMKYLHKALV 152
           VLKLGKSPSAAAAWINKNVALYIPATNITAIAVGSEVLTSFPNAGPFLIRAMKYLHKALV
Sbjct: 76  VLKLGKSPSAAAAWINKNVALYIPATNITAIAVGSEVLTSFPNAGPFLIRAMKYLHKALV 135

Query: 153 AANLNLQVKVSTPQSMDVVVTSFPPSTATFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYY 212
           AANLNLQVKVSTPQSMDVVVTSFPPSTATFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYY
Sbjct: 136 AANLNLQVKVSTPQSMDVVVTSFPPSTATFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYY 195

Query: 213 GYMKGNGIFPLDYALFRPLSINRRIVDPNTLLHYTNMFDAMLDATYHSIKSLNFSGIPIM 272
           GYMKGNGIFPLDYALFRPLSINRRIVDPNTLLHYTNMFDAMLDATYHS+KSLNFSGIPIM
Sbjct: 196 GYMKGNGIFPLDYALFRPLSINRRIVDPNTLLHYTNMFDAMLDATYHSVKSLNFSGIPIM 255

Query: 273 VTESGWPWSGGANETYATKKNAEIYVNNLIGRVLNGSGPPSEPTNPVNAYVYELFSEDQK 332
           VTESGWPWSGGANETYATKKNAEIYVNNL+GRVLNGSGPPSEPTNPVNAYVYELFSEDQK
Sbjct: 256 VTESGWPWSGGANETYATKKNAEIYVNNLLGRVLNGSGPPSEPTNPVNAYVYELFSEDQK 315

Query: 333 PGPMSEKNWGVLSTNGSAIYRLSLISSSVTGSSSVVYCVAKDGADPGKLQSGLNWACGQG 392
           PGPMSE NWGVLSTNGSAIYRLSLISSSVTGSSS+VYCVAKDGADPGKLQSGLNWACGQG
Sbjct: 316 PGPMSENNWGVLSTNGSAIYRLSLISSSVTGSSSIVYCVAKDGADPGKLQSGLNWACGQG 375

Query: 393 GANCSAIQPGQPCFLPDNVVNHASYAYNDYYQKMQGNGGTCDFEGTATLTNTNPS 448
           GANCSAIQPGQPCFLPDNVVNHASYAYNDYYQKMQG+GGTCDFEGTATLTNTNPS
Sbjct: 376 GANCSAIQPGQPCFLPDNVVNHASYAYNDYYQKMQGHGGTCDFEGTATLTNTNPS 430

[Close]

BLAST of Carg02757 vs. ExPASy TrEMBL
Match: A0A6J1K1M6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491597 PE=3 SV=1)

HSP 1 Score: 803.1 bits (2073), Expect = 5.9e-229
Identity = 402/415 (96.87%), Postives = 405/415 (97.59%), Query Frame = 0


Query: 33  AFVGINIGTHVSNLPPASKVVSMLKLYKITHVRLYDADVQLLKALANSSIEVMVGVTNEE 92
           +FVGINIGTHVSNLPPAS VVSMLKLYKITHVRLYDAD QLLKALANSSIEVMVGVTNEE
Sbjct: 16  SFVGINIGTHVSNLPPASNVVSMLKLYKITHVRLYDADAQLLKALANSSIEVMVGVTNEE 75

Query: 93  VLKLGKSPSAAAAWINKNVALYIPATNITAIAVGSEVLTSFPNAGPFLIRAMKYLHKALV 152
           VLKLGKSPSAAAAWINKNVALYIPA NITAIAVGSEVLTS PNAGPFLIRAMKYLHKALV
Sbjct: 76  VLKLGKSPSAAAAWINKNVALYIPAINITAIAVGSEVLTSIPNAGPFLIRAMKYLHKALV 135

Query: 153 AANLNLQVKVSTPQSMDVVVTSFPPSTATFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYY 212
           AANLNLQVKVSTPQSMDVV+TSFPPSTATFNSSLNSTIYPLLQFLK TKSYYMLNVYPYY
Sbjct: 136 AANLNLQVKVSTPQSMDVVLTSFPPSTATFNSSLNSTIYPLLQFLKTTKSYYMLNVYPYY 195

Query: 213 GYMKGNGIFPLDYALFRPLSINRRIVDPNTLLHYTNMFDAMLDATYHSIKSLNFSGIPIM 272
           GYMKGNGIFPLDYAL RPLSINRRIVDPNTLLHYTNMFDAMLDATYHSIKSLNFS IPI+
Sbjct: 196 GYMKGNGIFPLDYALLRPLSINRRIVDPNTLLHYTNMFDAMLDATYHSIKSLNFSDIPIV 255

Query: 273 VTESGWPWSGGANETYATKKNAEIYVNNLIGRVLNGSGPPSEPTNPVNAYVYELFSEDQK 332
           VTESGWPWSGGANETYATKKNAEIYVNNLI RVLNGSGPPSEPTNPVNAYVYELFSEDQK
Sbjct: 256 VTESGWPWSGGANETYATKKNAEIYVNNLISRVLNGSGPPSEPTNPVNAYVYELFSEDQK 315

Query: 333 PGPMSEKNWGVLSTNGSAIYRLSLISSSVTGSSSVVYCVAKDGADPGKLQSGLNWACGQG 392
           PGPMSEKNWGVL TNGSAIYRLSLISSSVTGSSSVVYCVAKDGADPGKLQSGLNWACGQG
Sbjct: 316 PGPMSEKNWGVLLTNGSAIYRLSLISSSVTGSSSVVYCVAKDGADPGKLQSGLNWACGQG 375

Query: 393 GANCSAIQPGQPCFLPDNVVNHASYAYNDYYQKMQGNGGTCDFEGTATLTNTNPS 448
           GANCSAIQPGQ CFLPDNVVNHASYAYNDYYQKMQGNGGTCDFEGTATLTNTNPS
Sbjct: 376 GANCSAIQPGQTCFLPDNVVNHASYAYNDYYQKMQGNGGTCDFEGTATLTNTNPS 430

[Close]

BLAST of Carg02757 vs. ExPASy TrEMBL
Match: A0A1S3BMB0 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103491590 PE=3 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 1.6e-221
Identity = 385/449 (85.75%), Postives = 412/449 (91.76%), Query Frame = 0


Query: 1   MFEVDVLVNWRRHEKWSAIVLLLVSAMFFHVR-AFVGINIGTHVSNLPPASKVVSMLKLY 60
           +FEVDV VNWR HEKW A++ LL S M FHVR AFVGINIGTHVS+LPPAS VVSMLK Y
Sbjct: 11  IFEVDVSVNWRMHEKWPAVLFLLTSVMTFHVRGAFVGINIGTHVSSLPPASDVVSMLKQY 70

Query: 61  KITHVRLYDADVQLLKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATN 120
           + THVRLYDA+ QLLKA ANSS EVMVGVTNEEVLK+GKSPSAAAAW+NKNV LY PATN
Sbjct: 71  EFTHVRLYDANAQLLKAFANSSTEVMVGVTNEEVLKIGKSPSAAAAWVNKNVVLYTPATN 130

Query: 121 ITAIAVGSEVLTSFPNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPST 180
           ITAIAVGSEVLTS PNA   LIRAMKYLHKALVAANLNLQ+KVSTPQSMDV++TSFPPST
Sbjct: 131 ITAIAVGSEVLTSIPNAARVLIRAMKYLHKALVAANLNLQIKVSTPQSMDVILTSFPPST 190

Query: 181 ATFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVD 240
           ATFNSSLNSTIY LLQFLK T SYYMLNVYPY+GY+KGNG FPLDYALF+PLSINR+IVD
Sbjct: 191 ATFNSSLNSTIYQLLQFLKKTNSYYMLNVYPYHGYIKGNGNFPLDYALFQPLSINRQIVD 250

Query: 241 PNTLLHYTNMFDAMLDATYHSIKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVN 300
           PNTLLHY NMFDAMLDATYHSIK+LNFS IPI+VTESGWPWS GA+E YATK+NAEIYVN
Sbjct: 251 PNTLLHYNNMFDAMLDATYHSIKALNFSDIPIVVTESGWPWSDGADEPYATKQNAEIYVN 310

Query: 301 NLIGRVLNGSGPPSEPTNPVNAYVYELFSEDQKPGPMSEKNWGVLSTNGSAIYRLSLISS 360
           NLI RVLNGSGPPSEPT PVN Y+YELFSEDQKP PMS+KNWGVLST GSAIYRLS +SS
Sbjct: 311 NLISRVLNGSGPPSEPTKPVNTYIYELFSEDQKPEPMSQKNWGVLSTKGSAIYRLSSLSS 370

Query: 361 SVTGSSSVVYCVAKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAY 420
            V GSSSVVYCVAKDGADPGKLQ+GLNWACGQGGANCSAIQPGQPCFLPDN+VNHASYAY
Sbjct: 371 RVAGSSSVVYCVAKDGADPGKLQNGLNWACGQGGANCSAIQPGQPCFLPDNIVNHASYAY 430

Query: 421 NDYYQKMQGNGGTCDFEGTATLTNTNPSK 449
           NDYYQKMQ NGGTC+F+GTATLTNTNPS+
Sbjct: 431 NDYYQKMQLNGGTCNFDGTATLTNTNPSR 459

[Close]

BLAST of Carg02757 vs. TAIR 10
Match: AT3G13560.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 560.8 bits (1444), Expect = 9.8e-160
Identity = 274/436 (62.84%), Postives = 351/436 (80.50%), Query Frame = 0


Query: 15  KWSA-IVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSMLKLYKITHVRLYDADVQL 74
           +W A  +LLL+S +     AF+G+NIGT ++N+PP S +V++LK  +ITHVRLYDA+  +
Sbjct: 5   RWFAEALLLLLSILACSNAAFIGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHM 64

Query: 75  LKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNITAIAVGSEVLTSF 134
           LKA AN+SIEVMVGVTNEE+LK+G+ PSAAAAW+NKNVA YIP+TNITAIAVGSEVLT+ 
Sbjct: 65  LKAFANTSIEVMVGVTNEEILKIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTI 124

Query: 135 PNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTATFNSSLNSTIYPL 194
           P+  P L  A+  +HKALVA+NLN +VKVS+P SMD++   FPPST+TF+ S N+T+Y L
Sbjct: 125 PHVAPILASALNNIHKALVASNLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSWNTTVYQL 184

Query: 195 LQFLKNTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDPNTLLHYTNMFDAM 254
           LQFLKNT S++MLN YPYYGY   NGIFPLDYALF+ LS  ++IVDPNTLLHY +MFDAM
Sbjct: 185 LQFLKNTGSFFMLNAYPYYGYTTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAM 244

Query: 255 LDATYHSIKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNNLIGRVLNGSGPPS 314
           +DA Y+S+++LNFS IP++VTE+GWP SGG++E  AT  NAE +  NLI RVLN SGPPS
Sbjct: 245 VDAAYYSMEALNFSKIPVVVTETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPS 304

Query: 315 EPTNPVNAYVYELFSEDQKPGPMSEKNWGVLSTNGSAIYRLSLI--SSSVTGSSSVVYCV 374
           +P  P+N Y+YEL++ED++ GP+SE+NWG+L  NG+++Y LSL   SSS   + S ++CV
Sbjct: 305 QPDIPINTYIYELYNEDKRSGPVSERNWGILFPNGTSVYPLSLSGGSSSAALNGSSMFCV 364

Query: 375 AKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYNDYYQKMQGNGG 434
           AK  AD  KL  GLNWACGQG ANC+AIQPGQPC+LP++V +HAS+A+NDYYQKM+  GG
Sbjct: 365 AKADADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPNDVKSHASFAFNDYYQKMKSAGG 424

Query: 435 TCDFEGTATLTNTNPS 448
           TCDF+GTA  T  +PS
Sbjct: 425 TCDFDGTAITTTRDPS 440

[Close]

BLAST of Carg02757 vs. TAIR 10
Match: AT3G13560.3 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 560.8 bits (1444), Expect = 9.8e-160
Identity = 274/436 (62.84%), Postives = 351/436 (80.50%), Query Frame = 0


Query: 15  KWSA-IVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSMLKLYKITHVRLYDADVQL 74
           +W A  +LLL+S +     AF+G+NIGT ++N+PP S +V++LK  +ITHVRLYDA+  +
Sbjct: 5   RWFAEALLLLLSILACSNAAFIGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHM 64

Query: 75  LKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNITAIAVGSEVLTSF 134
           LKA AN+SIEVMVGVTNEE+LK+G+ PSAAAAW+NKNVA YIP+TNITAIAVGSEVLT+ 
Sbjct: 65  LKAFANTSIEVMVGVTNEEILKIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTI 124

Query: 135 PNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTATFNSSLNSTIYPL 194
           P+  P L  A+  +HKALVA+NLN +VKVS+P SMD++   FPPST+TF+ S N+T+Y L
Sbjct: 125 PHVAPILASALNNIHKALVASNLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSWNTTVYQL 184

Query: 195 LQFLKNTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDPNTLLHYTNMFDAM 254
           LQFLKNT S++MLN YPYYGY   NGIFPLDYALF+ LS  ++IVDPNTLLHY +MFDAM
Sbjct: 185 LQFLKNTGSFFMLNAYPYYGYTTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAM 244

Query: 255 LDATYHSIKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNNLIGRVLNGSGPPS 314
           +DA Y+S+++LNFS IP++VTE+GWP SGG++E  AT  NAE +  NLI RVLN SGPPS
Sbjct: 245 VDAAYYSMEALNFSKIPVVVTETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPS 304

Query: 315 EPTNPVNAYVYELFSEDQKPGPMSEKNWGVLSTNGSAIYRLSLI--SSSVTGSSSVVYCV 374
           +P  P+N Y+YEL++ED++ GP+SE+NWG+L  NG+++Y LSL   SSS   + S ++CV
Sbjct: 305 QPDIPINTYIYELYNEDKRSGPVSERNWGILFPNGTSVYPLSLSGGSSSAALNGSSMFCV 364

Query: 375 AKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYNDYYQKMQGNGG 434
           AK  AD  KL  GLNWACGQG ANC+AIQPGQPC+LP++V +HAS+A+NDYYQKM+  GG
Sbjct: 365 AKADADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPNDVKSHASFAFNDYYQKMKSAGG 424

Query: 435 TCDFEGTATLTNTNPS 448
           TCDF+GTA  T  +PS
Sbjct: 425 TCDFDGTAITTTRDPS 440

[Close]

BLAST of Carg02757 vs. TAIR 10
Match: AT3G13560.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 560.8 bits (1444), Expect = 9.8e-160
Identity = 274/436 (62.84%), Postives = 351/436 (80.50%), Query Frame = 0


Query: 15  KWSA-IVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSMLKLYKITHVRLYDADVQL 74
           +W A  +LLL+S +     AF+G+NIGT ++N+PP S +V++LK  +ITHVRLYDA+  +
Sbjct: 5   RWFAEALLLLLSILACSNAAFIGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHM 64

Query: 75  LKALANSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNITAIAVGSEVLTSF 134
           LKA AN+SIEVMVGVTNEE+LK+G+ PSAAAAW+NKNVA YIP+TNITAIAVGSEVLT+ 
Sbjct: 65  LKAFANTSIEVMVGVTNEEILKIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTI 124

Query: 135 PNAGPFLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTATFNSSLNSTIYPL 194
           P+  P L  A+  +HKALVA+NLN +VKVS+P SMD++   FPPST+TF+ S N+T+Y L
Sbjct: 125 PHVAPILASALNNIHKALVASNLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSWNTTVYQL 184

Query: 195 LQFLKNTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDPNTLLHYTNMFDAM 254
           LQFLKNT S++MLN YPYYGY   NGIFPLDYALF+ LS  ++IVDPNTLLHY +MFDAM
Sbjct: 185 LQFLKNTGSFFMLNAYPYYGYTTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAM 244

Query: 255 LDATYHSIKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNNLIGRVLNGSGPPS 314
           +DA Y+S+++LNFS IP++VTE+GWP SGG++E  AT  NAE +  NLI RVLN SGPPS
Sbjct: 245 VDAAYYSMEALNFSKIPVVVTETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPS 304

Query: 315 EPTNPVNAYVYELFSEDQKPGPMSEKNWGVLSTNGSAIYRLSLI--SSSVTGSSSVVYCV 374
           +P  P+N Y+YEL++ED++ GP+SE+NWG+L  NG+++Y LSL   SSS   + S ++CV
Sbjct: 305 QPDIPINTYIYELYNEDKRSGPVSERNWGILFPNGTSVYPLSLSGGSSSAALNGSSMFCV 364

Query: 375 AKDGADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYNDYYQKMQGNGG 434
           AK  AD  KL  GLNWACGQG ANC+AIQPGQPC+LP++V +HAS+A+NDYYQKM+  GG
Sbjct: 365 AKADADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPNDVKSHASFAFNDYYQKMKSAGG 424

Query: 435 TCDFEGTATLTNTNPS 448
           TCDF+GTA  T  +PS
Sbjct: 425 TCDFDGTAITTTRDPS 440

[Close]

BLAST of Carg02757 vs. TAIR 10
Match: AT2G01630.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 443.4 bits (1139), Expect = 2.3e-124
Identity = 215/433 (49.65%), Postives = 304/433 (70.21%), Query Frame = 0


Query: 19  IVLLLVSAMFFHVRAFVGINIGTHVSNLPPASKVVSMLKLYKITHVRLYDADVQLLKALA 78
           + L L ++      + +G+NIGT V+N+P  ++VV++LK   I  VRLYDAD  +L A A
Sbjct: 7   LFLFLFASSALSQDSLIGVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADRSMLLAFA 66

Query: 79  NSSIEVMVGVTNEEVLKLGKSPSAAAAWINKNVALYIPATNITAIAVGSEVLTSFPNAGP 138
           ++ ++V++ V N+++L + +S + AA W+ +NVA Y PATNIT IAVGSEVLTS  NA  
Sbjct: 67  HTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSLTNAAS 126

Query: 139 FLIRAMKYLHKALVAANLNLQVKVSTPQSMDVVVTSFPPSTATFNSSLNSTIYPLLQFLK 198
            L+ A+KY+  ALV ANL+ Q+KVSTP S  +++ SFPPS A FN + +  I PLL+FL+
Sbjct: 127 VLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFNKTWDPVIVPLLKFLQ 186

Query: 199 NTKSYYMLNVYPYYGYMKGNGIFPLDYALFRPLSINRRIVDPNTLLHYTNMFDAMLDATY 258
           +T S  +LNVYPY+ Y++ NG+ PLDYALF+PL  N+  VD NTLLHYTN+FDA++DA Y
Sbjct: 187 STGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDAIVDAAY 246

Query: 259 HSIKSLNFSGIPIMVTESGWPWSGGANETYATKKNAEIYVNNLIGRVLNGSGPPSEPTNP 318
            ++  LNF+ IPI+VTESGWP  GG +E  AT +NA  Y +NLI  V+N +G P  P   
Sbjct: 247 FAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTPKHPGTA 306

Query: 319 VNAYVYELFSEDQKPGPMSEKNWGVLSTNGSAIYRLSLISSS---VTGSSSVVYCVAKDG 378
           V  Y+YEL++ED +PGP+SEKNWG+  TNG+ +Y L L  +       +++  +C+AK+ 
Sbjct: 307 VTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTLRLAGAGAILANDTTNQTFCIAKEK 366

Query: 379 ADPGKLQSGLNWACGQGGANCSAIQPGQPCFLPDNVVNHASYAYNDYYQKMQGNGGTCDF 438
            D   LQ+ L+WACG G  +CSA+  G+ C+ PD+VV H++YA+N YYQKM    G+CDF
Sbjct: 367 VDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFNAYYQKMGKASGSCDF 426

Query: 439 EGTATLTNTNPSK 449
           +G AT+T T+PS+
Sbjct: 427 KGVATVTTTDPSR 439

[Close]

BLAST of Carg02757 vs. TAIR 10
Match: AT1G66250.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 427.6 bits (1098), Expect = 1.3e-119
Identity = 210/418 (50.24%), Postives = 296/418 (70.81%), Query Frame = 0


Query: 33  AFVGINIGTHVSNLPPASKVVSMLKLYKITHVRLYDADVQLLKALANSSIEVMVGVTNEE 92
           +++G+NIGT +S++P  ++VV++LK  +I H+RLY+AD  LL ALAN+ I+V++ + N++
Sbjct: 30  SYIGVNIGTDLSDMPHPTQVVALLKAQEIRHIRLYNADPGLLIALANTGIKVIISIPNDQ 89

Query: 93  VLKLGKSPSAAAAWINKNVALYIPATNITAIAVGSEVLTSFPNAGPFLIRAMKYLHKALV 152
           +L +G+S S AA W+ +NV  + PAT ITA++VGSEVLTS  NA P L+ A+K +H AL+
Sbjct: 90  LLGIGQSNSTAANWVKRNVIAHYPATMITAVSVGSEVLTSLSNAAPVLVSAIKNVHAALL 149

Query: 153 AANLNLQVKVSTPQSMDVVVTSFPPSTATFNSSLNSTIYPLLQFLKNTKSYYMLNVYPYY 212
           +ANL+  +KVSTP S  +++  FPPS A FN SLN+ I PLL FL++T SY M+NVYPY 
Sbjct: 150 SANLDKLIKVSTPLSTSLILDPFPPSQAFFNRSLNAVIVPLLSFLQSTNSYLMVNVYPYI 209

Query: 213 GYMKGNGIFPLDYALFRPLSINRRIVDPNTLLHYTNMFDAMLDATYHSIKSLNFSGIPIM 272
            YM+ NG+ PLDYALF+P+  N+  VD NTL+ Y+N FDAM+DATY ++  LNF+ IP++
Sbjct: 210 DYMQSNGVIPLDYALFKPIPPNKEAVDANTLVRYSNAFDAMVDATYFAMAFLNFTNIPVL 269

Query: 273 VTESGWPWSGGANETYATKKNAEIYVNNLIGRVLNGSGPPSEPTNPVNAYVYELFSEDQK 332
           VTESGWP  G  NE  AT  NA  Y +NLI  VLN +G P  P   V+ Y+YEL++ED K
Sbjct: 270 VTESGWPSKGETNEPDATLDNANTYNSNLIRHVLNKTGTPKRPGIAVSTYIYELYNEDTK 329

Query: 333 PGPMSEKNWGVLSTNGSAIYRLSLISSS---VTGSSSVVYCVAKDGADPGKLQSGLNWAC 392
            G +SEKNWG+ + NG  +Y L L +S       +++  YC A++GAD   LQ+ L+WAC
Sbjct: 330 AG-LSEKNWGLFNANGEPVYVLRLTNSGSVLANDTTNQTYCTAREGADTKMLQAALDWAC 389

Query: 393 GQGGANCSAIQPGQPCFLPDNVVNHASYAYNDYYQKMQGNGGTCDFEGTATLTNTNPS 448
           G G  +CS I+ G+ C+ PDNVV HA+YA++ YY +   N   C+F G A++T T+PS
Sbjct: 390 GPGKIDCSPIKQGETCYEPDNVVAHANYAFDTYYHQTGNNPDACNFNGVASITTTDPS 446

The following BLAST results are available for this feature:

BLAST of Carg02757 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7035815.1	2.9e-262	100.00	Glucan endo-1,3-beta-glucosidase 4 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6605865.1	1.5e-255	99.78	Glucan endo-1,3-beta-glucosidase 4, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022957736.1	1.9e-253	98.43	glucan endo-1,3-beta-glucosidase 4-like isoform X1 [Cucurbita moschata]	[more]
XP_023533890.1	2.7e-252	98.43	glucan endo-1,3-beta-glucosidase 4-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022996336.1	1.5e-247	97.09	glucan endo-1,3-beta-glucosidase 4-like isoform X1 [Cucurbita maxima]	[more]

BLAST of Carg02757 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94CD8	1.4e-158	62.84	Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 ...	[more]
Q9ZU91	3.2e-123	49.65	Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ...	[more]
Q9C7U5	1.8e-118	50.24	Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 ...	[more]
O65399	3.8e-116	49.64	Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ...	[more]
Q8VYE5	4.4e-88	37.23	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]

BLAST of Carg02757 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H028	9.0e-254	98.43	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111459192 P...	[more]
A0A6J1K4F9	7.4e-248	97.09	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491597 PE=...	[more]
A0A6J1GZY9	3.6e-234	98.07	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111459192 P...	[more]
A0A6J1K1M6	5.9e-229	96.87	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491597 PE=...	[more]
A0A1S3BMB0	1.6e-221	85.75	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103491590 PE=3 SV...	[more]

BLAST of Carg02757 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G13560.1	9.8e-160	62.84	O-Glycosyl hydrolases family 17 protein	[more]
AT3G13560.3	9.8e-160	62.84	O-Glycosyl hydrolases family 17 protein	[more]
AT3G13560.2	9.8e-160	62.84	O-Glycosyl hydrolases family 17 protein	[more]
AT2G01630.1	2.3e-124	49.65	O-Glycosyl hydrolases family 17 protein	[more]
AT1G66250.1	1.3e-119	50.24	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 368..449 e-value: 1.6E-40 score: 150.6
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 369..440 e-value: 2.2E-19 score: 69.9
None	No IPR available	GENE3D	1.20.58.1040		coord: 363..456 e-value: 1.3E-10 score: 43.5
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 34..361 e-value: 2.1E-109 score: 367.4
None	No IPR available	PANTHER	PTHR32227:SF274	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE 4	coord: 13..448
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 35..354 e-value: 2.2E-75 score: 254.2
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 13..448
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 35..355

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg02757-RA	Carg02757-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds