Carg02696 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg02696
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Sacchrp_dh_NADP domain-containing protein
Location	Carg_Chr02: 7319443 .. 7322330 (-)
RNA-Seq Expression	Carg02696
Synteny	Carg02696

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTGCCGGTGCGGAGAAGAGCGGCAAACAATGGCGGGAGCTTTGAAAAGCTTTTCTCCGATAGCCATGATCAATGCCGACACTGAACTTCTGGTTCAATTGCCTCAGAATGATTGCAATCCTAGGGTTTTGGTACTCGGAGGCACCGGTCGGGTCGGAACCTCCATTGCCATTGCTCTCTTCAAGTTCTCGTCATTGGCGGTCTCAACAGGTGATTCTTCTGTGTTTATTTTAACTCGGTGAGTTTCATTTGTAGGTAGGTTTGAATTTGACTTATTTCGATTCTTTTCATTTCTGTATCTCTAAGTTATTCCTTAAAGTTGGTTTATCCGGCGTGTTCTCTCGTTTAGATCCTTTTTCATTATGAAACTAAACTGTTGTTTACCGATTGTTAATCTGTGGATTTCGAGCCTTTTCTAAATTGTGATGCATAATGATATAAGGTGCGGCGTGCTGTGAAACGGAAAAAGGTGCTGCTATGGTTGCGACAGTTGGGAGAAACTCTCGATTTGTTGAAGTCGATGCTGAGAATGTAAAATCATTGGAGGCTGCTTTGAGAGGTATACTGATTCTTCTAATTAGTTTATGATTTCAACAGTCGTCTTTGATTCTATTTCTTCTCCAATTTCCATTTCGTGAGTGAAATATGAAGCTCCTAGTCATCCTGTGAGCTTTTCCTCCTTCAAATGGAGTTTTAAATTTAAAACTTAGCGGTCAAATTACAATCCAATAGTTTAATATTCTGAAACATAAAGTTGTACCCACGAGGAAATAATTTCATACATAAAGTTTTACCTTCGAGGCTACGAAAAGAGGTGTGAAATGCAAGAGATCGCTGTTCAAGGAATTTTCTCTTTATCTCAAACTTTGAATAGCGGTATCCTTTTCCTTTCTTTGTTATCATTATTATTAAAAATTCTCTGTAATTTGCGTTGCCTATTTATGGAAGTTCCAACACATGGAGTCTCCTGCACCTGTTGACGCCTTTCTTTTTTATTGTTTAAACTACACACCGTTATTACTCTTTTTTAAAGTTTTAGCTCTGAGTCTCTGACAAATAACATGGTATTGTCTCTAGATGTCGTGTATGGTTAATTTCACGACCTTTTTTTTTGTTGACTCCAAAGACTGTCAAGCGACCAGAACTTTGGTGGTTCTGATGAGCTTTTGACCTCGGTGTAAAATGCAGATGTAATTTAATATAATATCATGGTTTATTTATAATAAGATTAGTGAAATTACATGTAGGGATCACTTCCTCACCCCCCTTAGTTGTTTTTTATTTTCTTACCATGGAATTATTTTGTAAGTAACTGATTTTTGTGGTCAAACAATTTAAAATGGAAGTATTATACATACATTTGCCCCTTTTGATTAATGAAATATGTCTATCTGGAACAAAACACGTGAAAATCTTGCACCCTAAACTAAGGGCATTCAAATCTTTAACATATTGCCACCAGGGGTGCTTAACTGAAATCTTTAGCTGTTACTACAGGTGTGAATCCTGTAGTTCATATTGCTGGGTCATTCCAACAGACAGAGAAGTGCACCGTACTTGAAGCTTCCATAAATATCCAGGAAAGAGCTGCATTTGCTTTAAAAATCTTGCTTTTATGATCATATATTGACGACGTTCTTTGTCTATCTGCATACAGCCTACGTTGGTGTTTCATACAAACTATTCACAGAATGCAAAAGCTTTCAAGAATAAAGCTATGGAGGCAAATATTCCAGCTATTACAACTGCATTTATCCTGGAGTGAGCAATGGTACATAATGTACTTATCGTGCACTTTTCTTATGTGGATAGTTCTTATTATATGGTGCTGCTAAATCTCTTATATTGAATGCAATATTCAACCTATTCATTTTCCTTGCACAAAGTATAGGAAAGATATTGTGTAAACTAATGGGCGAGTGGTAGGTTGAAGTTGAAGTTTTGTTTTCATGGGCAATAAAAACAACATATTCAAGTCAATGCTTCGTCTAACTGACAGTCTTTTCTGTTTCACCGAGCCAGTAATGGTGGCAGAGCTAGTACGTGCTGGAGAGATGAAAGCAGGGGTGATCCCGATAGGCTAAAGAGAAGTCCCTGATAATCATGCAACTATTGTGTAAACCTCACGACTGATGAAGCATACTTTTCCCCATCACTTATAATTCAATGCTGTATTTGTACAATAGGATGTAAACAAACTCCCCATGCCAACAGAGAATTAACAATTTATAATCTTCCGGATTTGGTGTATTCTTTAATAACCTCTGTATTTCCTTCGAGGAACCTCTGTATTTCACGATTTTATATGAAAATGGAAGAAATCAGGGTCAAAAGATTTGTGATTTTTAATGCCGTCATGTTTTATATTCAGCATAACCTTCAGTTAAACTAGAAAACTTAGAGGTCGTTAGTATAACTAATTATAAAAATTGATATGGTGCACTTTCTTCAGCAACTAAGCGTGAATTATTATCTCAGATTCTACCTGCACTGCAGGCAATGGTGGTGCTGGTCCATCTTTATTAGCTACTAGTTTCTTGCTGCTGGGAGAGAAGGTGGTCGCCTATAATAAAGGTTAGTGTCAAAACTGAAACTTGTTTGTCTTTGTAATATTCCAGCACTTGATGATTTTGCTGGAGAGCGTGTATCCATGAGGGTGAGAATCAAATTTCTTATATTTATACACACAAATTTCTGGTGAAAGAAGACGTAGCTTTATCAACCATTTGATAATTTGATTTCTGATTTATCAGGTGTTGGAAGTAGTTGATTTATGAAGAATGCCCCTTTTTCTTTTTCTGTTTTTGGTTTAATTCTGATTAGATGAGGCTACTTCATGGATAGCAGGTGACGAGAAGGAAAGTCGTGTAGTTTATGTAAGAATGTGA

mRNA sequence

CTTGCCGGTGCGGAGAAGAGCGGCAAACAATGGCGGGAGCTTTGAAAAGCTTTTCTCCGATAGCCATGATCAATGCCGACACTGAACTTCTGGTTCAATTGCCTCAGAATGATTGCAATCCTAGGGTTTTGGTACTCGGAGGCACCGGTCGGGTCGGAACCTCCATTGCCATTGCTCTCTTCAAGTTCTCGTCATTGGCGGTCTCAACAGGTGCGGCGTGCTGTGAAACGGAAAAAGGTGCTGCTATGGTTGCGACAGTTGGGAGAAACTCTCGATTTGTTGAAGTCGATGCTGAGAATGTAAAATCATTGGAGGCTGCTTTGAGAGGTGTGAATCCTGTAGTTCATATTGCTGGGTCATTCCAACAGACAGAGAAGTGCACCCCTACGTTGGTGTTTCATACAAACTATTCACAGAATGCAAAAGCTTTCAAGAATAAAGCTATGGAGGCAAATATTCCAGCTATTACAACTGCATTTATCCTGGATAATGGTGGCAGAGCTAGCAATGGTGGTGCTGGTCCATCTTTATTAGCTACTAGTTTCTTGCTGCTGGGAGAGAAGGTGGTCGCCTATAATAAAGATGAGGCTACTTCATGGATAGCAGGTGACGAGAAGGAAAGTCGTGTAGTTTATGTAAGAATGTGA

Coding sequence (CDS)

ATGGCGGGAGCTTTGAAAAGCTTTTCTCCGATAGCCATGATCAATGCCGACACTGAACTTCTGGTTCAATTGCCTCAGAATGATTGCAATCCTAGGGTTTTGGTACTCGGAGGCACCGGTCGGGTCGGAACCTCCATTGCCATTGCTCTCTTCAAGTTCTCGTCATTGGCGGTCTCAACAGGTGCGGCGTGCTGTGAAACGGAAAAAGGTGCTGCTATGGTTGCGACAGTTGGGAGAAACTCTCGATTTGTTGAAGTCGATGCTGAGAATGTAAAATCATTGGAGGCTGCTTTGAGAGGTGTGAATCCTGTAGTTCATATTGCTGGGTCATTCCAACAGACAGAGAAGTGCACCCCTACGTTGGTGTTTCATACAAACTATTCACAGAATGCAAAAGCTTTCAAGAATAAAGCTATGGAGGCAAATATTCCAGCTATTACAACTGCATTTATCCTGGATAATGGTGGCAGAGCTAGCAATGGTGGTGCTGGTCCATCTTTATTAGCTACTAGTTTCTTGCTGCTGGGAGAGAAGGTGGTCGCCTATAATAAAGATGAGGCTACTTCATGGATAGCAGGTGACGAGAAGGAAAGTCGTGTAGTTTATGTAAGAATGTGA

Protein sequence

MAGALKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKFSSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRGVNPVVHIAGSFQQTEKCTPTLVFHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGRASNGGAGPSLLATSFLLLGEKVVAYNKDEATSWIAGDEKESRVVYVRM

Homology

[Close]

BLAST of Carg02696 vs. NCBI nr
Match: KAG7035876.1 (hypothetical protein SDJN02_02675 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 400.2 bits (1027), Expect = 1.1e-107
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 1   MAGALKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKFSSLAVST 60
           MAGALKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKFSSLAVST
Sbjct: 1   MAGALKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKFSSLAVST 60

Query: 61  GAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRGVNPVVHIAGSFQQTEKCTPT 120
           GAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRGVNPVVHIAGSFQQTEKCTPT
Sbjct: 61  GAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRGVNPVVHIAGSFQQTEKCTPT 120

Query: 121 LVFHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGRASNGGAGPSLLATSFLLLGEKVV 180
           LVFHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGRASNGGAGPSLLATSFLLLGEKVV
Sbjct: 121 LVFHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGRASNGGAGPSLLATSFLLLGEKVV 180

Query: 181 AYNKDEATSWIAGDEKESRVVYVRM 206
           AYNKDEATSWIAGDEKESRVVYVRM
Sbjct: 181 AYNKDEATSWIAGDEKESRVVYVRM 205

[Close]

BLAST of Carg02696 vs. NCBI nr
Match: KAG6605927.1 (hypothetical protein SDJN03_03244, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 278.1 bits (710), Expect = 6.1e-71
Identity = 160/214 (74.77%), Postives = 164/214 (76.64%), Query Frame = 0


Query: 1   MAGALKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKFSSLAVST 60
           MAGALKSFSPIAMINADTELLVQLPQNDCNP VLVLGGTGRVGTSIAIALFKFSSLAV+T
Sbjct: 1   MAGALKSFSPIAMINADTELLVQLPQNDCNPTVLVLGGTGRVGTSIAIALFKFSSLAVAT 60

Query: 61  GAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRGVNPVVHIAGSFQQTEKCTPT 120
           GAACCETEK AAMVATVGRNSRFVEVDAENVKSLEAALRGV+PVVHIAGSFQQTEKCT +
Sbjct: 61  GAACCETEKNAAMVATVGRNSRFVEVDAENVKSLEAALRGVDPVVHIAGSFQQTEKCT-S 120

Query: 121 LVFHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGRAS---------NGGAGPSLLATS 180
            +FH                             NGGRAS         NGGAGPSLLATS
Sbjct: 121 FLFHR--------------------------ASNGGRASTCWRDESRGNGGAGPSLLATS 179

Query: 181 FLLLGEKVVAYNKDEATSWIAGDEKESRVVYVRM 206
           FLLLGEKVVAYNK        GDEKESRVVYVRM
Sbjct: 181 FLLLGEKVVAYNK--------GDEKESRVVYVRM 179

[Close]

BLAST of Carg02696 vs. NCBI nr
Match: XP_022957944.1 (uncharacterized protein LOC111459328 [Cucurbita moschata] >XP_022957945.1 uncharacterized protein LOC111459328 [Cucurbita moschata] >XP_022957946.1 uncharacterized protein LOC111459328 [Cucurbita moschata] >XP_022957947.1 uncharacterized protein LOC111459328 [Cucurbita moschata] >XP_022957948.1 uncharacterized protein LOC111459328 [Cucurbita moschata])

HSP 1 Score: 214.5 bits (545), Expect = 8.3e-52
Identity = 111/118 (94.07%), Postives = 113/118 (95.76%), Query Frame = 0


Query: 1   MAGALKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKFSSLAVST 60
           MAGALKSFSPIAMIN DTELLVQLPQNDCN RVLVLG TGRVGTSIAIALFKFSSLAV+T
Sbjct: 1   MAGALKSFSPIAMINVDTELLVQLPQNDCNSRVLVLGSTGRVGTSIAIALFKFSSLAVAT 60

Query: 61  GAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRGVNPVVHIAGSFQQTEKCT 119
           GAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALR V+PVVH AGSFQQTEKCT
Sbjct: 61  GAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRDVDPVVHTAGSFQQTEKCT 118

[Close]

BLAST of Carg02696 vs. NCBI nr
Match: KAG6570406.1 (hypothetical protein SDJN03_29321, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 208.0 bits (528), Expect = 7.8e-50
Identity = 131/220 (59.55%), Postives = 143/220 (65.00%), Query Frame = 0


Query: 1   MAGA---LKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKF-SSL 60
           MAGA   LKSFSP+AM  ADTEL +QLPQN  N RVLVLGGTGRVG S A AL KF   L
Sbjct: 1   MAGAFFRLKSFSPMAMATADTELPLQLPQNVRNSRVLVLGGTGRVGASTATALSKFCPDL 60

Query: 61  AVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRGVNPVVHIAGSFQQTEK 120
            ++ G      EKG AMVAT+GRNSRFVEVD EN K LEAALR V+ VVH AG FQQTEK
Sbjct: 61  QIAIGGR--NREKGEAMVATLGRNSRFVEVDVENAKMLEAALRDVDLVVHTAGPFQQTEK 120

Query: 121 CTPTLV---FHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGR---------------- 180
           CT   V     +NYSQNAK+FKNKA+EANIPAITTA I                      
Sbjct: 121 CTTAYVDVCDDSNYSQNAKSFKNKAIEANIPAITTAGIYPGVSNVMAAELVRVARDESKC 180

Query: 181 -----------ASNGGAGPSLLATSFLLLGEKVVAYNKDE 187
                      A  GGAGP++LATSFLLLGE+VVAYNK E
Sbjct: 181 EPERLRFYYYTAGTGGAGPTILATSFLLLGEEVVAYNKGE 218

[Close]

BLAST of Carg02696 vs. NCBI nr
Match: KAG7010280.1 (hypothetical protein SDJN02_27073, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 203.0 bits (515), Expect = 2.5e-48
Identity = 131/224 (58.48%), Postives = 142/224 (63.39%), Query Frame = 0


Query: 1   MAGA---LKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKF-SSL 60
           MAGA   LKSFSP+AM  ADTEL +QLPQN  N RVLVLGGTGRVG S A AL KF   L
Sbjct: 1   MAGAFFRLKSFSPMAMATADTELPLQLPQNVRNSRVLVLGGTGRVGASTATALSKFCPDL 60

Query: 61  AVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRGVNP----VVHIAGSFQ 120
            ++ G      EKG AMVAT+GRNSRFVEVD EN K LEAALR V      VVH AG FQ
Sbjct: 61  QIAIGGR--NREKGEAMVATLGRNSRFVEVDVENAKMLEAALRAVTTDVDLVVHTAGPFQ 120

Query: 121 QTEKCTPTLV---FHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGR------------ 180
           QTEKCT   V     +NYSQNAK+FKNKA+EANIPAITTA I                  
Sbjct: 121 QTEKCTTAYVDVCDDSNYSQNAKSFKNKAIEANIPAITTAGIYPGVSNVMAAELVRVARD 180

Query: 181 ---------------ASNGGAGPSLLATSFLLLGEKVVAYNKDE 187
                          A  GGAGP++LATSFLLLGE+VVAYNK E
Sbjct: 181 ESKCEPERLRFYYYTAGTGGAGPTILATSFLLLGEEVVAYNKGE 222

[Close]

BLAST of Carg02696 vs. ExPASy TrEMBL
Match: A0A6J1H1Q4 (uncharacterized protein LOC111459328 OS=Cucurbita moschata OX=3662 GN=LOC111459328 PE=4 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 4.0e-52
Identity = 111/118 (94.07%), Postives = 113/118 (95.76%), Query Frame = 0


Query: 1   MAGALKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKFSSLAVST 60
           MAGALKSFSPIAMIN DTELLVQLPQNDCN RVLVLG TGRVGTSIAIALFKFSSLAV+T
Sbjct: 1   MAGALKSFSPIAMINVDTELLVQLPQNDCNSRVLVLGSTGRVGTSIAIALFKFSSLAVAT 60

Query: 61  GAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRGVNPVVHIAGSFQQTEKCT 119
           GAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALR V+PVVH AGSFQQTEKCT
Sbjct: 61  GAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRDVDPVVHTAGSFQQTEKCT 118

[Close]

BLAST of Carg02696 vs. ExPASy TrEMBL
Match: A0A6J1FYX2 (uncharacterized protein LOC111449104 OS=Cucurbita moschata OX=3662 GN=LOC111449104 PE=4 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 4.6e-48
Identity = 129/229 (56.33%), Postives = 143/229 (62.45%), Query Frame = 0


Query: 1   MAGA---LKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKF-SSL 60
           MAGA   LKSFSP+AM  ADTEL +QLP+N  N RVLVLGGTGRVG S A AL KF   L
Sbjct: 1   MAGAFFRLKSFSPMAMATADTELPLQLPKNVRNSRVLVLGGTGRVGASTATALSKFCPDL 60

Query: 61  AVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRGVNPVVHIAGSFQQTEK 120
            ++ G      EKG AMVAT+GRNSRFVEVD EN K LEAALR V+ VVH AG FQQTEK
Sbjct: 61  QIAIGGR--NREKGEAMVATLGRNSRFVEVDVENAKMLEAALRDVDLVVHTAGPFQQTEK 120

Query: 121 CT------------PTLVFHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGR------- 180
           CT              +   +NYSQNAK+FKNKA+EANIPAITTA I             
Sbjct: 121 CTVLEASINTKTAYVDVCDDSNYSQNAKSFKNKAIEANIPAITTAGIYPGVSNVMAAELV 180

Query: 181 --------------------ASNGGAGPSLLATSFLLLGEKVVAYNKDE 187
                               A  GGAGP++LATSFLLLGE+VVAYNK E
Sbjct: 181 RVARDESKCEPERLRFYYYTAGTGGAGPTILATSFLLLGEEVVAYNKGE 227

[Close]

BLAST of Carg02696 vs. ExPASy TrEMBL
Match: A0A6J1J8E6 (uncharacterized protein LOC111484409 OS=Cucurbita maxima OX=3661 GN=LOC111484409 PE=4 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.8e-47
Identity = 128/229 (55.90%), Postives = 142/229 (62.01%), Query Frame = 0


Query: 1   MAGA---LKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKF-SSL 60
           MAGA   LKSFSP+AM  ADTEL +QLPQN  N RVLVLGGTGRVG S A AL KF   L
Sbjct: 1   MAGAFFRLKSFSPMAMATADTELPLQLPQNVRNSRVLVLGGTGRVGASTATALSKFCPDL 60

Query: 61  AVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRGVNPVVHIAGSFQQTEK 120
            ++ G      EKG AMVAT+GRNSRFVEVD EN K LEAALR V+ VVH AG FQQTEK
Sbjct: 61  QIAIGGR--NREKGEAMVATLGRNSRFVEVDVENAKMLEAALRDVDLVVHTAGPFQQTEK 120

Query: 121 CTP------------TLVFHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGR------- 180
           CT              +   + YSQNAK+FKNKA++ANIPAITTA I             
Sbjct: 121 CTVLEASINTKTAYIDVCDDSKYSQNAKSFKNKAIDANIPAITTAGIYPGVSNVMAAELV 180

Query: 181 --------------------ASNGGAGPSLLATSFLLLGEKVVAYNKDE 187
                               A  GGAGP++LATSFLLLGE+VVAYNK E
Sbjct: 181 RVARDESKCEPERLRFYYYTAGTGGAGPTILATSFLLLGEEVVAYNKGE 227

[Close]

BLAST of Carg02696 vs. ExPASy TrEMBL
Match: A0A6J1DG83 (uncharacterized protein LOC111020567 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111020567 PE=4 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 2.5e-46
Identity = 130/229 (56.77%), Postives = 145/229 (63.32%), Query Frame = 0


Query: 1   MAGA---LKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKF-SSL 60
           MAG+   LKSFSP+AM  A+TEL +QLPQN  N RVLVLGGTGRVG S AIAL +F   L
Sbjct: 1   MAGSLLPLKSFSPMAMAIAETELPLQLPQNVRNSRVLVLGGTGRVGGSTAIALSRFCPDL 60

Query: 61  AVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRGVNPVVHIAGSFQQTEK 120
            +  G      EKGAAMVAT+G NSRFVEVD ENVKSLEAALR V+ VVH AG FQQT+K
Sbjct: 61  QIVIGGR--NREKGAAMVATLGGNSRFVEVDVENVKSLEAALRDVDLVVHTAGPFQQTKK 120

Query: 121 CT------------PTLVFHTNYSQNAKAFKNKAMEANIPAITTAFI-----------LD 180
           CT              +   TNYS NAKA KNKA++ANIPAITTA I           L 
Sbjct: 121 CTVLEASIDTKTAYVDVCDDTNYSWNAKALKNKAIDANIPAITTAGIYPGVSNVMAAELV 180

Query: 181 NGGR----------------ASNGGAGPSLLATSFLLLGEKVVAYNKDE 187
              R                A  GGAGP++LATSFLLLGE+VVAYNK E
Sbjct: 181 RAARDESKAEPERLRFYYYTAGTGGAGPTILATSFLLLGEEVVAYNKGE 227

[Close]

BLAST of Carg02696 vs. ExPASy TrEMBL
Match: A0A6J1DHK9 (uncharacterized protein LOC111020567 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111020567 PE=4 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 2.5e-46
Identity = 130/229 (56.77%), Postives = 145/229 (63.32%), Query Frame = 0


Query: 1   MAGA---LKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKF-SSL 60
           MAG+   LKSFSP+AM  A+TEL +QLPQN  N RVLVLGGTGRVG S AIAL +F   L
Sbjct: 1   MAGSLLPLKSFSPMAMAIAETELPLQLPQNVRNSRVLVLGGTGRVGGSTAIALSRFCPDL 60

Query: 61  AVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRGVNPVVHIAGSFQQTEK 120
            +  G      EKGAAMVAT+G NSRFVEVD ENVKSLEAALR V+ VVH AG FQQT+K
Sbjct: 61  QIVIGGR--NREKGAAMVATLGGNSRFVEVDVENVKSLEAALRDVDLVVHTAGPFQQTKK 120

Query: 121 CT------------PTLVFHTNYSQNAKAFKNKAMEANIPAITTAFI-----------LD 180
           CT              +   TNYS NAKA KNKA++ANIPAITTA I           L 
Sbjct: 121 CTVLEASIDTKTAYVDVCDDTNYSWNAKALKNKAIDANIPAITTAGIYPGVSNVMAAELV 180

Query: 181 NGGR----------------ASNGGAGPSLLATSFLLLGEKVVAYNKDE 187
              R                A  GGAGP++LATSFLLLGE+VVAYNK E
Sbjct: 181 RAARDESKAEPERLRFYYYTAGTGGAGPTILATSFLLLGEEVVAYNKGE 227

[Close]

BLAST of Carg02696 vs. TAIR 10
Match: AT1G50450.1 (Saccharopine dehydrogenase )

HSP 1 Score: 119.0 bits (297), Expect = 4.4e-27
Identity = 85/197 (43.15%), Postives = 104/197 (52.79%), Query Frame = 0


Query: 30  NPRVLVLGGTGRVGTSIAIALFKF-SSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDA 89
           N RVLVLGGTGRVG S A AL K    L +  G      EKG AMVA +G NS F +VD 
Sbjct: 38  NYRVLVLGGTGRVGGSTATALSKLCPELKIVVGGR--NREKGEAMVAKLGENSEFSQVDI 97

Query: 90  ENVKSLEAALRGVNPVVHIAGSFQQTEKCTP------------TLVFHTNYSQNAKAFKN 149
            + K LE +LR V+ VVH AG FQQ  +CT              +   T+Y+  AK+ + 
Sbjct: 98  NDAKMLETSLRDVDLVVHAAGPFQQAPRCTVLEAAIKTKTAYLDVCDDTSYAFRAKSLEA 157

Query: 150 KAMEANIPAITTAFIL------------------DNGG---------RASNGGAGPSLLA 187
           +A+ ANIPA+TTA I                   D G           A  GGAGP++LA
Sbjct: 158 EAIAANIPALTTAGIYPGVSNVMAAEMVAAARSEDKGKPEKLRFSYYTAGTGGAGPTILA 217

The following BLAST results are available for this feature:

BLAST of Carg02696 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7035876.1	1.1e-107	100.00	hypothetical protein SDJN02_02675 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6605927.1	6.1e-71	74.77	hypothetical protein SDJN03_03244, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022957944.1	8.3e-52	94.07	uncharacterized protein LOC111459328 [Cucurbita moschata] >XP_022957945.1 unchar...	[more]
KAG6570406.1	7.8e-50	59.55	hypothetical protein SDJN03_29321, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7010280.1	2.5e-48	58.48	hypothetical protein SDJN02_27073, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of Carg02696 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg02696 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H1Q4	4.0e-52	94.07	uncharacterized protein LOC111459328 OS=Cucurbita moschata OX=3662 GN=LOC1114593...	[more]
A0A6J1FYX2	4.6e-48	56.33	uncharacterized protein LOC111449104 OS=Cucurbita moschata OX=3662 GN=LOC1114491...	[more]
A0A6J1J8E6	1.8e-47	55.90	uncharacterized protein LOC111484409 OS=Cucurbita maxima OX=3661 GN=LOC111484409...	[more]
A0A6J1DG83	2.5e-46	56.77	uncharacterized protein LOC111020567 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1DHK9	2.5e-46	56.77	uncharacterized protein LOC111020567 isoform X2 OS=Momordica charantia OX=3673 G...	[more]

BLAST of Carg02696 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G50450.1	4.4e-27	43.15	Saccharopine dehydrogenase	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002225	3-beta hydroxysteroid dehydrogenase/isomerase	PFAM	PF01073	3Beta_HSD	coord: 34..127 e-value: 3.4E-5 score: 23.0
None	No IPR available	GENE3D	3.40.50.720		coord: 29..147 e-value: 1.8E-10 score: 42.7
None	No IPR available	PANTHER	PTHR43796	CARBOXYNORSPERMIDINE SYNTHASE	coord: 157..187 coord: 21..149
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 31..144

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg02696-RA	Carg02696-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006694	steroid biosynthetic process
cellular_component	GO:0016020	membrane
molecular_function	GO:0003854	3-beta-hydroxy-delta5-steroid dehydrogenase activity
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor