Carg02672 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg02672
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Molybdopterin synthase catalytic subunit
Location	Carg_Chr02: 7485242 .. 7485970 (+)
RNA-Seq Expression	Carg02672
Synteny	Carg02672

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTTGGAGGAGAGAAATCTTGTTGAAATCTTGGAAGAGAACGTCCCAATCGACATAGTAAAGTACATCAAGTTTGTTAGTGTTCCCCAGGCCGGCGCTATCTCTACATTTTCAGGCACTACTCGGGACAATTTTGAAGGCAAGACGGTGGTGGAACTGCGTTACGAGGCTTATGTACCAATGGCAATACGGAGCCTCAAATCCATTTGTTCATCTGCAAGGTCGTCTTGGAACCTTCATTCAATCGCAGTTGCGCACCGACTAGGTCCTGTTCCTGTTGGGGAGATCAGTGTGTTCATTGCAGTTTCAGCTGTTCATCGAGCTGATGCAATGGATTCTTGCCGGTTCGTGATCGATGAGCTCAAGGCGTCTGTGCCAATATGGAAAAAGGAGGTTTATGATAATGGGGAGGTTTGGAAGGAGAATTCTGAGTTTATGGACAGAAGATTGGAGATTGGGAAGAAGGAAGGAGATGTGAATGAAGAAAATGAAGCTGAAATTCTTCCAAGGCATGGTAGGAAAAGCTGCTGTGGCTCAAAGGTTAAGGTGACTGAAGAATCTTCTTCTAGCTGCATTAGTTCAGAGCATGAAAAGAATCATCAACACAATGTTGAGAAGGAAAACGAAGAAGATGAAGAAGCTCAAAGGCATGTAAGGAAAAGCTGCTGTGGCTCGAAAGTTAAGGTGAATGAACGAGGAATCACCATTGATGACAGTGATGGATAG

mRNA sequence

ATGGCTTTGGAGGAGAGAAATCTTGTTGAAATCTTGGAAGAGAACGTCCCAATCGACATAGTAAAGTACATCAAGTTTGTTAGTGTTCCCCAGGCCGGCGCTATCTCTACATTTTCAGGCACTACTCGGGACAATTTTGAAGGCAAGACGGTGGTGGAACTGCGTTACGAGGCTTATGTACCAATGGCAATACGGAGCCTCAAATCCATTTGTTCATCTGCAAGGTCGTCTTGGAACCTTCATTCAATCGCAGTTGCGCACCGACTAGGTCCTGTTCCTGTTGGGGAGATCAGTGTGTTCATTGCAGTTTCAGCTGTTCATCGAGCTGATGCAATGGATTCTTGCCGGTTCGTGATCGATGAGCTCAAGGCGTCTGTGCCAATATGGAAAAAGGAGGTTTATGATAATGGGGAGGTTTGGAAGGAGAATTCTGAGTTTATGGACAGAAGATTGGAGATTGGGAAGAAGGAAGGAGATGTGAATGAAGAAAATGAAGCTGAAATTCTTCCAAGGCATGGTAGGAAAAGCTGCTGTGGCTCAAAGGTTAAGGTGACTGAAGAATCTTCTTCTAGCTGCATTAGTTCAGAGCATGAAAAGAATCATCAACACAATGTTGAGAAGGAAAACGAAGAAGATGAAGAAGCTCAAAGGCATGTAAGGAAAAGCTGCTGTGGCTCGAAAGTTAAGGTGAATGAACGAGGAATCACCATTGATGACAGTGATGGATAG

Coding sequence (CDS)

ATGGCTTTGGAGGAGAGAAATCTTGTTGAAATCTTGGAAGAGAACGTCCCAATCGACATAGTAAAGTACATCAAGTTTGTTAGTGTTCCCCAGGCCGGCGCTATCTCTACATTTTCAGGCACTACTCGGGACAATTTTGAAGGCAAGACGGTGGTGGAACTGCGTTACGAGGCTTATGTACCAATGGCAATACGGAGCCTCAAATCCATTTGTTCATCTGCAAGGTCGTCTTGGAACCTTCATTCAATCGCAGTTGCGCACCGACTAGGTCCTGTTCCTGTTGGGGAGATCAGTGTGTTCATTGCAGTTTCAGCTGTTCATCGAGCTGATGCAATGGATTCTTGCCGGTTCGTGATCGATGAGCTCAAGGCGTCTGTGCCAATATGGAAAAAGGAGGTTTATGATAATGGGGAGGTTTGGAAGGAGAATTCTGAGTTTATGGACAGAAGATTGGAGATTGGGAAGAAGGAAGGAGATGTGAATGAAGAAAATGAAGCTGAAATTCTTCCAAGGCATGGTAGGAAAAGCTGCTGTGGCTCAAAGGTTAAGGTGACTGAAGAATCTTCTTCTAGCTGCATTAGTTCAGAGCATGAAAAGAATCATCAACACAATGTTGAGAAGGAAAACGAAGAAGATGAAGAAGCTCAAAGGCATGTAAGGAAAAGCTGCTGTGGCTCGAAAGTTAAGGTGAATGAACGAGGAATCACCATTGATGACAGTGATGGATAG

Protein sequence

MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYVPMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVIDELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVNEENEAEILPRHGRKSCCGSKVKVTEESSSSCISSEHEKNHQHNVEKENEEDEEAQRHVRKSCCGSKVKVNERGITIDDSDG

Homology

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BLAST of Carg02672 vs. NCBI nr
Match: KAG7035899.1 (Molybdopterin synthase catalytic subunit, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 473.8 bits (1218), Expect = 9.0e-130
Identity = 242/242 (100.00%), Postives = 242/242 (100.00%), Query Frame = 0


Query: 1   MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 60
           MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYV
Sbjct: 1   MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 60

Query: 61  PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID 120
           PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID
Sbjct: 61  PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID 120

Query: 121 ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVNEENEAEILPRHGRKSCCGS 180
           ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVNEENEAEILPRHGRKSCCGS
Sbjct: 121 ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVNEENEAEILPRHGRKSCCGS 180

Query: 181 KVKVTEESSSSCISSEHEKNHQHNVEKENEEDEEAQRHVRKSCCGSKVKVNERGITIDDS 240
           KVKVTEESSSSCISSEHEKNHQHNVEKENEEDEEAQRHVRKSCCGSKVKVNERGITIDDS
Sbjct: 181 KVKVTEESSSSCISSEHEKNHQHNVEKENEEDEEAQRHVRKSCCGSKVKVNERGITIDDS 240

Query: 241 DG 243
           DG
Sbjct: 241 DG 242

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BLAST of Carg02672 vs. NCBI nr
Match: XP_022957855.1 (molybdopterin synthase catalytic subunit [Cucurbita moschata] >XP_022957856.1 molybdopterin synthase catalytic subunit [Cucurbita moschata] >XP_022957857.1 molybdopterin synthase catalytic subunit [Cucurbita moschata] >XP_023532736.1 molybdopterin synthase catalytic subunit [Cucurbita pepo subsp. pepo] >XP_023532737.1 molybdopterin synthase catalytic subunit [Cucurbita pepo subsp. pepo] >XP_023532738.1 molybdopterin synthase catalytic subunit [Cucurbita pepo subsp. pepo])

HSP 1 Score: 452.2 bits (1162), Expect = 2.8e-123
Identity = 231/232 (99.57%), Postives = 232/232 (100.00%), Query Frame = 0


Query: 1   MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 60
           MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYV
Sbjct: 1   MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 60

Query: 61  PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID 120
           PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID
Sbjct: 61  PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID 120

Query: 121 ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVNEENEAEILPRHGRKSCCGS 180
           ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDV+EENEAEILPRHGRKSCCGS
Sbjct: 121 ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVSEENEAEILPRHGRKSCCGS 180

Query: 181 KVKVTEESSSSCISSEHEKNHQHNVEKENEEDEEAQRHVRKSCCGSKVKVNE 233
           KVKVTEESSSSCISSEHEKNHQHNVEKENEEDEEAQRHVRKSCCGSKVKVNE
Sbjct: 181 KVKVTEESSSSCISSEHEKNHQHNVEKENEEDEEAQRHVRKSCCGSKVKVNE 232

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BLAST of Carg02672 vs. NCBI nr
Match: XP_022995139.1 (molybdopterin synthase catalytic subunit [Cucurbita maxima] >XP_022995140.1 molybdopterin synthase catalytic subunit [Cucurbita maxima] >XP_022995141.1 molybdopterin synthase catalytic subunit [Cucurbita maxima])

HSP 1 Score: 443.0 bits (1138), Expect = 1.7e-120
Identity = 226/232 (97.41%), Postives = 230/232 (99.14%), Query Frame = 0


Query: 1   MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 60
           MALEERNLVEILEENVPIDIVKYIKFVSVPQAGA+STFSGTTRDNFEGKTVVELRYEAYV
Sbjct: 1   MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAVSTFSGTTRDNFEGKTVVELRYEAYV 60

Query: 61  PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID 120
           PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID
Sbjct: 61  PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID 120

Query: 121 ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVNEENEAEILPRHGRKSCCGS 180
           ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDV+EENEAEILPRHGRK+CCGS
Sbjct: 121 ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVSEENEAEILPRHGRKTCCGS 180

Query: 181 KVKVTEESSSSCISSEHEKNHQHNVEKENEEDEEAQRHVRKSCCGSKVKVNE 233
           KVKVTEESSSSCISSEHEKNHQHNVEKENEED E QRH+RKSCCGSKVKVNE
Sbjct: 181 KVKVTEESSSSCISSEHEKNHQHNVEKENEED-EVQRHIRKSCCGSKVKVNE 231

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BLAST of Carg02672 vs. NCBI nr
Match: XP_038900956.1 (molybdopterin synthase catalytic subunit [Benincasa hispida])

HSP 1 Score: 372.1 bits (954), Expect = 3.7e-99
Identity = 197/233 (84.55%), Postives = 209/233 (89.70%), Query Frame = 0


Query: 1   MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 60
           MA EER LVEILEEN+PID+ KYI FVS PQAGAISTFSGTTRDNFEGKTVVELRYEAYV
Sbjct: 1   MASEERILVEILEENIPIDMAKYITFVSAPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 60

Query: 61  PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID 120
           PMAIRS+KSICSSARSSWNLHSIAV HRLGPVPVGEISVFIAVSAVHRADAMD CRFVID
Sbjct: 61  PMAIRSIKSICSSARSSWNLHSIAVVHRLGPVPVGEISVFIAVSAVHRADAMDCCRFVID 120

Query: 121 ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVNEENEAEILPRHGRKSCCGS 180
           ELKA VPIWKKEVYDNGEVWKENSEFMDRRLE+ KK+GD N ENE E++ RH RKSCCGS
Sbjct: 121 ELKACVPIWKKEVYDNGEVWKENSEFMDRRLELVKKQGDQN-ENETELV-RHSRKSCCGS 180

Query: 181 KVKVTEESSSSCISSEHEK-NHQHNVEKENEEDEEAQRHVRKSCCGSKVKVNE 233
           KVKV  E SSSCISS+HE+ N  HNVEKENEE  + QR+ RKSCCGSKVK+NE
Sbjct: 181 KVKVMNEESSSCISSDHEEGNRHHNVEKENEE-AQVQRNGRKSCCGSKVKLNE 230

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BLAST of Carg02672 vs. NCBI nr
Match: XP_004140180.1 (molybdopterin synthase catalytic subunit [Cucumis sativus] >KGN48068.1 hypothetical protein Csa_002904 [Cucumis sativus])

HSP 1 Score: 365.5 bits (937), Expect = 3.4e-97
Identity = 194/237 (81.86%), Postives = 209/237 (88.19%), Query Frame = 0


Query: 1   MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 60
           M  EER  VEILE+N+PID+ KYI FVS PQAGAISTFSGTTRDNFEGKTVVELRYEAYV
Sbjct: 1   MTSEERTFVEILEDNIPIDMAKYITFVSAPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 60

Query: 61  PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID 120
           PMAIRS+KSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMD CRFVID
Sbjct: 61  PMAIRSIKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDCCRFVID 120

Query: 121 ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVNEENEAEILPRHGRKSCCGS 180
           ELKA VPIWKKEVYDNGEVWKENSEFMDRRLE+  KEGD + ENE  ++ RH RKSCCGS
Sbjct: 121 ELKACVPIWKKEVYDNGEVWKENSEFMDRRLELVNKEGDDHNENETAVV-RHNRKSCCGS 180

Query: 181 KVKVTEESSSSC-ISSEHEK-NHQHNVEKENE-EDEEA--QRHVRKSCCGSKVKVNE 233
           KVKV  E  S C ISS+HE+ NHQHNV+ ENE E+EEA  QR++RKSCCGSKVK+NE
Sbjct: 181 KVKVMNEEPSKCIISSDHEEGNHQHNVKNENENENEEAQLQRNIRKSCCGSKVKLNE 236

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BLAST of Carg02672 vs. ExPASy Swiss-Prot
Match: O22827 (Molybdopterin synthase catalytic subunit OS=Arabidopsis thaliana OX=3702 GN=MOCS2 PE=2 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 2.2e-70
Identity = 128/200 (64.00%), Postives = 164/200 (82.00%), Query Frame = 0


Query: 1   MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 60
           M+ EE+NL+EILEE   +D+VKYI +VS PQAGAI+TFSGTTRD FEGKTV+ELRYEAYV
Sbjct: 1   MSAEEKNLIEILEEGHKVDVVKYIDYVSAPQAGAIATFSGTTRDMFEGKTVLELRYEAYV 60

Query: 61  PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID 120
           PMA R L SIC++ARS+W++H IAVAHRLGPVPVGE SV IAVS+VHRAD +D+C+F+ID
Sbjct: 61  PMATRCLSSICTTARSTWDIHKIAVAHRLGPVPVGETSVLIAVSSVHRADGLDACKFLID 120

Query: 121 ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVNEENEAEILPRHGRKSCCGS 180
           ELKASVPIWKKEVY NGE+WKENSEFM++RLE+ +K   + ++    ++  H R+ CCGS
Sbjct: 121 ELKASVPIWKKEVYTNGEIWKENSEFMEKRLELAEKRDSIVKKT---VVEEHRRRGCCGS 180

Query: 181 KVKVTEESSSSCISSEHEKN 201
           KV+V E+     I+ +++ +
Sbjct: 181 KVRVEEDEEHKDITGDNKSS 197

[Close]

BLAST of Carg02672 vs. ExPASy Swiss-Prot
Match: A2X0R4 (Molybdopterin synthase catalytic subunit OS=Oryza sativa subsp. indica OX=39946 GN=MOCS2 PE=3 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 2.1e-49
Identity = 108/186 (58.06%), Postives = 131/186 (70.43%), Query Frame = 0


Query: 2   ALEERNLVEILEE-NVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 61
           A EE +LVEIL+E +  +DI +Y+  V    AGAI+TF GTTRD+FEG+ VVELRYEAY 
Sbjct: 12  ATEEEDLVEILDEGSGRLDIARYVDHVRDLAAGAIATFEGTTRDSFEGRRVVELRYEAYG 71

Query: 62  PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID 121
            MA R L +I   AR++ +L  +AVAHRLG VP GE SVF+A SAVHRADAM++CR+VID
Sbjct: 72  AMARRRLAAILREARAAHSLRRLAVAHRLGTVPAGEASVFVAASAVHRADAMEACRYVID 131

Query: 122 ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVNEENEAEILPRHGRKSCCGS 181
           E+KASVPIWKKEVYD+GEVWKEN EF+DR       +G           P  G   CCGS
Sbjct: 132 EVKASVPIWKKEVYDDGEVWKENREFLDRT----TTDGTTASSPAPATRPAKG-GGCCGS 191

Query: 182 KVKVTE 187
           KV+  E
Sbjct: 192 KVRANE 192

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BLAST of Carg02672 vs. ExPASy Swiss-Prot
Match: Q6Z2X3 (Molybdopterin synthase catalytic subunit OS=Oryza sativa subsp. japonica OX=39947 GN=MOCS2 PE=2 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 2.8e-49
Identity = 108/186 (58.06%), Postives = 131/186 (70.43%), Query Frame = 0


Query: 2   ALEERNLVEILEE-NVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 61
           A EE +LVEIL+E +  +DI +Y+  V    AGAI+TF GTTRD+FEG+ VVELRYEAY 
Sbjct: 12  ATEEEDLVEILDEGSGRLDIARYVDHVRDLAAGAIATFEGTTRDSFEGRRVVELRYEAYG 71

Query: 62  PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID 121
            MA R L +I   AR++ +L  +AVAHRLG VP GE SVF+A SAVHRADAM++CR+VID
Sbjct: 72  AMARRRLAAILREARAAHSLRRLAVAHRLGTVPAGEASVFVAASAVHRADAMEACRYVID 131

Query: 122 ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVNEENEAEILPRHGRKSCCGS 181
           E+KASVPIWKKEVYD+GEVWKEN EF+DR       +G           P  G   CCG 
Sbjct: 132 EVKASVPIWKKEVYDDGEVWKENREFLDRT----TTDGTTASSPAPATRPAKG-GGCCGR 191

Query: 182 KVKVTE 187
           KV+V E
Sbjct: 192 KVRVNE 192

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BLAST of Carg02672 vs. ExPASy Swiss-Prot
Match: O96007 (Molybdopterin synthase catalytic subunit OS=Homo sapiens OX=9606 GN=MOCS2 PE=1 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.1e-32
Identity = 69/142 (48.59%), Postives = 92/142 (64.79%), Query Frame = 0


Query: 4   EERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYVPMA 63
           + ++++    E + +D V   + V  P  GAIS F GTTR+NFEGK V+ L YEAY+PMA
Sbjct: 42  KSKDVINFTAEKLSVDEVS--QLVISPLCGAISLFVGTTRNNFEGKKVISLEYEAYLPMA 101

Query: 64  IRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVIDELK 123
              ++ ICS  R  W +  IAV HRLG VPV E S+ IAVS+ HRA ++++  + ID LK
Sbjct: 102 ENEVRKICSDIRQKWPVKHIAVFHRLGLVPVSEASIIIAVSSAHRAASLEAVSYAIDTLK 161

Query: 124 ASVPIWKKEVYDNGEVWKENSE 146
           A VPIWKKE+Y+    WK N E
Sbjct: 162 AKVPIWKKEIYEESSTWKGNKE 181

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BLAST of Carg02672 vs. ExPASy Swiss-Prot
Match: A4FUY7 (Molybdopterin synthase catalytic subunit OS=Bos taurus OX=9913 GN=MOCS2 PE=2 SV=2)

HSP 1 Score: 138.7 bits (348), Expect = 9.0e-32
Identity = 70/143 (48.95%), Postives = 94/143 (65.73%), Query Frame = 0


Query: 4   EERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYVPMA 63
           + +++++   E + +D V   + V  P  GAIS F GTTR+NFEGK V+ L YEAY+PMA
Sbjct: 42  KSKDIIKFTSEKLSVDEVS--QLVISPLCGAISLFVGTTRNNFEGKKVISLEYEAYLPMA 101

Query: 64  IRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVIDELK 123
              ++ ICS  R  W +  IAV HRLG VPV E SV IAVS+ HRA ++++  + ID LK
Sbjct: 102 ENEIRKICSDMRQKWPVRHIAVFHRLGLVPVTEASVIIAVSSAHRAASLEAVSYAIDALK 161

Query: 124 ASVPIWKKEVY-DNGEVWKENSE 146
           A VPIWKKE+Y ++   WK N E
Sbjct: 162 ARVPIWKKEIYEESSSSWKRNKE 182

[Close]

BLAST of Carg02672 vs. ExPASy TrEMBL
Match: A0A6J1H1T2 (Molybdopterin synthase catalytic subunit OS=Cucurbita moschata OX=3662 GN=LOC111459270 PE=3 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.4e-123
Identity = 231/232 (99.57%), Postives = 232/232 (100.00%), Query Frame = 0


Query: 1   MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 60
           MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYV
Sbjct: 1   MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 60

Query: 61  PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID 120
           PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID
Sbjct: 61  PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID 120

Query: 121 ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVNEENEAEILPRHGRKSCCGS 180
           ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDV+EENEAEILPRHGRKSCCGS
Sbjct: 121 ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVSEENEAEILPRHGRKSCCGS 180

Query: 181 KVKVTEESSSSCISSEHEKNHQHNVEKENEEDEEAQRHVRKSCCGSKVKVNE 233
           KVKVTEESSSSCISSEHEKNHQHNVEKENEEDEEAQRHVRKSCCGSKVKVNE
Sbjct: 181 KVKVTEESSSSCISSEHEKNHQHNVEKENEEDEEAQRHVRKSCCGSKVKVNE 232

[Close]

BLAST of Carg02672 vs. ExPASy TrEMBL
Match: A0A6J1K163 (Molybdopterin synthase catalytic subunit OS=Cucurbita maxima OX=3661 GN=LOC111490770 PE=3 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 8.2e-121
Identity = 226/232 (97.41%), Postives = 230/232 (99.14%), Query Frame = 0


Query: 1   MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 60
           MALEERNLVEILEENVPIDIVKYIKFVSVPQAGA+STFSGTTRDNFEGKTVVELRYEAYV
Sbjct: 1   MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAVSTFSGTTRDNFEGKTVVELRYEAYV 60

Query: 61  PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID 120
           PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID
Sbjct: 61  PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID 120

Query: 121 ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVNEENEAEILPRHGRKSCCGS 180
           ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDV+EENEAEILPRHGRK+CCGS
Sbjct: 121 ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVSEENEAEILPRHGRKTCCGS 180

Query: 181 KVKVTEESSSSCISSEHEKNHQHNVEKENEEDEEAQRHVRKSCCGSKVKVNE 233
           KVKVTEESSSSCISSEHEKNHQHNVEKENEED E QRH+RKSCCGSKVKVNE
Sbjct: 181 KVKVTEESSSSCISSEHEKNHQHNVEKENEED-EVQRHIRKSCCGSKVKVNE 231

[Close]

BLAST of Carg02672 vs. ExPASy TrEMBL
Match: A0A0A0KEX7 (Molybdopterin synthase catalytic subunit OS=Cucumis sativus OX=3659 GN=Csa_6G428010 PE=3 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 1.7e-97
Identity = 194/237 (81.86%), Postives = 209/237 (88.19%), Query Frame = 0


Query: 1   MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 60
           M  EER  VEILE+N+PID+ KYI FVS PQAGAISTFSGTTRDNFEGKTVVELRYEAYV
Sbjct: 1   MTSEERTFVEILEDNIPIDMAKYITFVSAPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 60

Query: 61  PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID 120
           PMAIRS+KSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMD CRFVID
Sbjct: 61  PMAIRSIKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDCCRFVID 120

Query: 121 ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVNEENEAEILPRHGRKSCCGS 180
           ELKA VPIWKKEVYDNGEVWKENSEFMDRRLE+  KEGD + ENE  ++ RH RKSCCGS
Sbjct: 121 ELKACVPIWKKEVYDNGEVWKENSEFMDRRLELVNKEGDDHNENETAVV-RHNRKSCCGS 180

Query: 181 KVKVTEESSSSC-ISSEHEK-NHQHNVEKENE-EDEEA--QRHVRKSCCGSKVKVNE 233
           KVKV  E  S C ISS+HE+ NHQHNV+ ENE E+EEA  QR++RKSCCGSKVK+NE
Sbjct: 181 KVKVMNEEPSKCIISSDHEEGNHQHNVKNENENENEEAQLQRNIRKSCCGSKVKLNE 236

[Close]

BLAST of Carg02672 vs. ExPASy TrEMBL
Match: A0A5A7V2E3 (Molybdopterin synthase catalytic subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00470 PE=3 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 2.4e-96
Identity = 189/235 (80.43%), Postives = 207/235 (88.09%), Query Frame = 0


Query: 1   MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 60
           MA EER LVEILEEN+PID+ KYI FVS PQAGAISTFSGTTRDNFEGKTVVELRYEAYV
Sbjct: 1   MASEERTLVEILEENIPIDMAKYITFVSAPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 60

Query: 61  PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID 120
           PMAIRS+KSICSSARSSWNLHSIAVAHRLGPVPVG +SVFIAVSAVHRADAMD CRFVID
Sbjct: 61  PMAIRSIKSICSSARSSWNLHSIAVAHRLGPVPVGGVSVFIAVSAVHRADAMDCCRFVID 120

Query: 121 ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVNEENEAEILPRHGRKSCCGS 180
           ELKA VPIWKKEVYDNGEVWKENSEF DRRLE+  KEG  ++ENE  ++ RH RKSCCGS
Sbjct: 121 ELKACVPIWKKEVYDNGEVWKENSEFTDRRLELVNKEGHDHDENETAVV-RHNRKSCCGS 180

Query: 181 KVKVTEESSSSC-ISSEHEK-NHQHNVEKENEEDE-EAQRHVRKSCCGSKVKVNE 233
           KVK   E  S+C ISS+HE+ NHQHNV+ ENE +E + QR++RKSCCGSKVK+NE
Sbjct: 181 KVKAMNEEPSNCIISSDHEEGNHQHNVKNENENEEAQLQRNIRKSCCGSKVKLNE 234

[Close]

BLAST of Carg02672 vs. ExPASy TrEMBL
Match: A0A1S3BMF2 (Molybdopterin synthase catalytic subunit OS=Cucumis melo OX=3656 GN=LOC103491449 PE=3 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 2.4e-96
Identity = 189/235 (80.43%), Postives = 207/235 (88.09%), Query Frame = 0


Query: 1   MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 60
           MA EER LVEILEEN+PID+ KYI FVS PQAGAISTFSGTTRDNFEGKTVVELRYEAYV
Sbjct: 1   MASEERTLVEILEENIPIDMAKYITFVSAPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 60

Query: 61  PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID 120
           PMAIRS+KSICSSARSSWNLHSIAVAHRLGPVPVG +SVFIAVSAVHRADAMD CRFVID
Sbjct: 61  PMAIRSIKSICSSARSSWNLHSIAVAHRLGPVPVGGVSVFIAVSAVHRADAMDCCRFVID 120

Query: 121 ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVNEENEAEILPRHGRKSCCGS 180
           ELKA VPIWKKEVYDNGEVWKENSEF DRRLE+  KEG  ++ENE  ++ RH RKSCCGS
Sbjct: 121 ELKACVPIWKKEVYDNGEVWKENSEFTDRRLELVNKEGHDHDENETAVV-RHNRKSCCGS 180

Query: 181 KVKVTEESSSSC-ISSEHEK-NHQHNVEKENEEDE-EAQRHVRKSCCGSKVKVNE 233
           KVK   E  S+C ISS+HE+ NHQHNV+ ENE +E + QR++RKSCCGSKVK+NE
Sbjct: 181 KVKAMNEEPSNCIISSDHEEGNHQHNVKNENENEEAQLQRNIRKSCCGSKVKLNE 234

[Close]

BLAST of Carg02672 vs. TAIR 10
Match: AT2G43760.1 (molybdopterin biosynthesis MoaE family protein )

HSP 1 Score: 266.9 bits (681), Expect = 1.6e-71
Identity = 128/200 (64.00%), Postives = 164/200 (82.00%), Query Frame = 0


Query: 1   MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 60
           M+ EE+NL+EILEE   +D+VKYI +VS PQAGAI+TFSGTTRD FEGKTV+ELRYEAYV
Sbjct: 1   MSAEEKNLIEILEEGHKVDVVKYIDYVSAPQAGAIATFSGTTRDMFEGKTVLELRYEAYV 60

Query: 61  PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID 120
           PMA R L SIC++ARS+W++H IAVAHRLGPVPVGE SV IAVS+VHRAD +D+C+F+ID
Sbjct: 61  PMATRCLSSICTTARSTWDIHKIAVAHRLGPVPVGETSVLIAVSSVHRADGLDACKFLID 120

Query: 121 ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVNEENEAEILPRHGRKSCCGS 180
           ELKASVPIWKKEVY NGE+WKENSEFM++RLE+ +K   + ++    ++  H R+ CCGS
Sbjct: 121 ELKASVPIWKKEVYTNGEIWKENSEFMEKRLELAEKRDSIVKKT---VVEEHRRRGCCGS 180

Query: 181 KVKVTEESSSSCISSEHEKN 201
           KV+V E+     I+ +++ +
Sbjct: 181 KVRVEEDEEHKDITGDNKSS 197

[Close]

BLAST of Carg02672 vs. TAIR 10
Match: AT2G43760.2 (molybdopterin biosynthesis MoaE family protein )

HSP 1 Score: 266.9 bits (681), Expect = 1.6e-71
Identity = 128/200 (64.00%), Postives = 164/200 (82.00%), Query Frame = 0


Query: 1   MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 60
           M+ EE+NL+EILEE   +D+VKYI +VS PQAGAI+TFSGTTRD FEGKTV+ELRYEAYV
Sbjct: 1   MSAEEKNLIEILEEGHKVDVVKYIDYVSAPQAGAIATFSGTTRDMFEGKTVLELRYEAYV 60

Query: 61  PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID 120
           PMA R L SIC++ARS+W++H IAVAHRLGPVPVGE SV IAVS+VHRAD +D+C+F+ID
Sbjct: 61  PMATRCLSSICTTARSTWDIHKIAVAHRLGPVPVGETSVLIAVSSVHRADGLDACKFLID 120

Query: 121 ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVNEENEAEILPRHGRKSCCGS 180
           ELKASVPIWKKEVY NGE+WKENSEFM++RLE+ +K   + ++    ++  H R+ CCGS
Sbjct: 121 ELKASVPIWKKEVYTNGEIWKENSEFMEKRLELAEKRDSIVKKT---VVEEHRRRGCCGS 180

Query: 181 KVKVTEESSSSCISSEHEKN 201
           KV+V E+     I+ +++ +
Sbjct: 181 KVRVEEDEEHKDITGDNKSS 197

[Close]

BLAST of Carg02672 vs. TAIR 10
Match: AT2G43760.3 (molybdopterin biosynthesis MoaE family protein )

HSP 1 Score: 266.9 bits (681), Expect = 1.6e-71
Identity = 128/200 (64.00%), Postives = 164/200 (82.00%), Query Frame = 0


Query: 1   MALEERNLVEILEENVPIDIVKYIKFVSVPQAGAISTFSGTTRDNFEGKTVVELRYEAYV 60
           M+ EE+NL+EILEE   +D+VKYI +VS PQAGAI+TFSGTTRD FEGKTV+ELRYEAYV
Sbjct: 1   MSAEEKNLIEILEEGHKVDVVKYIDYVSAPQAGAIATFSGTTRDMFEGKTVLELRYEAYV 60

Query: 61  PMAIRSLKSICSSARSSWNLHSIAVAHRLGPVPVGEISVFIAVSAVHRADAMDSCRFVID 120
           PMA R L SIC++ARS+W++H IAVAHRLGPVPVGE SV IAVS+VHRAD +D+C+F+ID
Sbjct: 61  PMATRCLSSICTTARSTWDIHKIAVAHRLGPVPVGETSVLIAVSSVHRADGLDACKFLID 120

Query: 121 ELKASVPIWKKEVYDNGEVWKENSEFMDRRLEIGKKEGDVNEENEAEILPRHGRKSCCGS 180
           ELKASVPIWKKEVY NGE+WKENSEFM++RLE+ +K   + ++    ++  H R+ CCGS
Sbjct: 121 ELKASVPIWKKEVYTNGEIWKENSEFMEKRLELAEKRDSIVKKT---VVEEHRRRGCCGS 180

Query: 181 KVKVTEESSSSCISSEHEKN 201
           KV+V E+     I+ +++ +
Sbjct: 181 KVRVEEDEEHKDITGDNKSS 197

The following BLAST results are available for this feature:

BLAST of Carg02672 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7035899.1	9.0e-130	100.00	Molybdopterin synthase catalytic subunit, partial [Cucurbita argyrosperma subsp....	[more]
XP_022957855.1	2.8e-123	99.57	molybdopterin synthase catalytic subunit [Cucurbita moschata] >XP_022957856.1 mo...	[more]
XP_022995139.1	1.7e-120	97.41	molybdopterin synthase catalytic subunit [Cucurbita maxima] >XP_022995140.1 moly...	[more]
XP_038900956.1	3.7e-99	84.55	molybdopterin synthase catalytic subunit [Benincasa hispida]	[more]
XP_004140180.1	3.4e-97	81.86	molybdopterin synthase catalytic subunit [Cucumis sativus] >KGN48068.1 hypotheti...	[more]

BLAST of Carg02672 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22827	2.2e-70	64.00	Molybdopterin synthase catalytic subunit OS=Arabidopsis thaliana OX=3702 GN=MOCS...	[more]
A2X0R4	2.1e-49	58.06	Molybdopterin synthase catalytic subunit OS=Oryza sativa subsp. indica OX=39946 ...	[more]
Q6Z2X3	2.8e-49	58.06	Molybdopterin synthase catalytic subunit OS=Oryza sativa subsp. japonica OX=3994...	[more]
O96007	1.1e-32	48.59	Molybdopterin synthase catalytic subunit OS=Homo sapiens OX=9606 GN=MOCS2 PE=1 S...	[more]
A4FUY7	9.0e-32	48.95	Molybdopterin synthase catalytic subunit OS=Bos taurus OX=9913 GN=MOCS2 PE=2 SV=...	[more]

BLAST of Carg02672 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H1T2	1.4e-123	99.57	Molybdopterin synthase catalytic subunit OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1K163	8.2e-121	97.41	Molybdopterin synthase catalytic subunit OS=Cucurbita maxima OX=3661 GN=LOC11149...	[more]
A0A0A0KEX7	1.7e-97	81.86	Molybdopterin synthase catalytic subunit OS=Cucumis sativus OX=3659 GN=Csa_6G428...	[more]
A0A5A7V2E3	2.4e-96	80.43	Molybdopterin synthase catalytic subunit OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A1S3BMF2	2.4e-96	80.43	Molybdopterin synthase catalytic subunit OS=Cucumis melo OX=3656 GN=LOC103491449...	[more]

BLAST of Carg02672 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G43760.1	1.6e-71	64.00	molybdopterin biosynthesis MoaE family protein	[more]
AT2G43760.2	1.6e-71	64.00	molybdopterin biosynthesis MoaE family protein	[more]
AT2G43760.3	1.6e-71	64.00	molybdopterin biosynthesis MoaE family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003448	Molybdopterin biosynthesis MoaE	PFAM	PF02391	MoaE	coord: 17..124 e-value: 8.7E-39 score: 132.3
IPR003448	Molybdopterin biosynthesis MoaE	CDD	cd00756	MoaE	coord: 18..141 e-value: 2.78258E-56 score: 173.857
IPR036563	Molybdopterin biosynthesis MoaE subunit superfamily	GENE3D	3.90.1170.40	Molybdopterin biosynthesis MoaE subunit	coord: 1..143 e-value: 6.6E-55 score: 187.2
IPR036563	Molybdopterin biosynthesis MoaE subunit superfamily	SUPERFAMILY	54690	Molybdopterin synthase subunit MoaE	coord: 17..146
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 192..219
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 189..219
None	No IPR available	PANTHER	PTHR23404:SF11	MOLYBDOPTERIN SYNTHASE CATALYTIC SUBUNIT	coord: 1..192
None	No IPR available	PANTHER	PTHR23404	MOLYBDOPTERIN SYNTHASE RELATED	coord: 1..192
IPR028888	Molybdopterin synthase catalytic subunit, eukaryotes	HAMAP	MF_03052	MOC2B	coord: 9..149 score: 51.22871

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg02672-RA	Carg02672-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007015	actin filament organization
biological_process	GO:0006777	Mo-molybdopterin cofactor biosynthetic process
biological_process	GO:0010119	regulation of stomatal movement
cellular_component	GO:0005829	cytosol
cellular_component	GO:0019008	molybdopterin synthase complex
molecular_function	GO:0003779	actin binding
molecular_function	GO:0030366	molybdopterin synthase activity