Carg02669 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg02669 vs. NCBI nr
Match: KAG7035902.1 (Cysteine desulfurase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 946.0 bits (2444), Expect = 1.2e-271
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0

Query: 1   MEKMMEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRCSFRRFSSPFSVSASASTARETD 60
           MEKMMEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRCSFRRFSSPFSVSASASTARETD
Sbjct: 1   MEKMMEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRCSFRRFSSPFSVSASASTARETD 60

Query: 61  NLHLGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVH 120
           NLHLGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVH
Sbjct: 61  NLHLGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVH 120

Query: 121 RGMHFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDE 180
           RGMHFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDE
Sbjct: 121 RGMHFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDE 180

Query: 181 VIITVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSN 240
           VIITVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSN
Sbjct: 181 VIITVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSN 240

Query: 241 VLASVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGF 300
           VLASVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGF
Sbjct: 241 VLASVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGF 300

Query: 301 LYGKSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSG 360
           LYGKSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSG
Sbjct: 301 LYGKSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSG 360

Query: 361 IGMQKIHSYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFL 420
           IGMQKIHSYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFL
Sbjct: 361 IGMQKIHSYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFL 420

Query: 421 DQQHGVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDVLTCYLTERSFTKTI 476
           DQQHGVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDVLTCYLTERSFTKTI
Sbjct: 421 DQQHGVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDVLTCYLTERSFTKTI 475

BLAST of Carg02669 vs. NCBI nr
Match: XP_022957848.1 (cysteine desulfurase 1, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 911.8 bits (2355), Expect = 2.5e-261
Identity = 457/458 (99.78%), Postives = 457/458 (99.78%), Query Frame = 0

Query: 4   MMEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRCSFRRFSSPFSVSASASTARETDNLH 63
           MMEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRCSFRRFSSPFSVSASASTARETDNLH
Sbjct: 1   MMEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRCSFRRFSSPFSVSASASTARETDNLH 60

Query: 64  LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM 123
           LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM
Sbjct: 61  LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM 120

Query: 124 HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII 183
           HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII
Sbjct: 121 HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII 180

Query: 184 TVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSNVLA 243
           TVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSNVLA
Sbjct: 181 TVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSNVLA 240

Query: 244 SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG 303
           SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG
Sbjct: 241 SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG 300

Query: 304 KSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM 363
           KSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM
Sbjct: 301 KSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM 360

Query: 364 QKIHSYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFLDQQ 423
           QKIHSYEVELANYLYESLRAVPNIHIYGPAPT SVQRAALCSFNIENIHPTDIASFLDQQ
Sbjct: 361 QKIHSYEVELANYLYESLRAVPNIHIYGPAPTGSVQRAALCSFNIENIHPTDIASFLDQQ 420

Query: 424 HGVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDV 462
           HGVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDV
Sbjct: 421 HGVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDV 458

BLAST of Carg02669 vs. NCBI nr
Match: KAG6605954.1 (Nuclear pore complex protein NUP96, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 899.8 bits (2324), Expect = 1.0e-257
Identity = 457/469 (97.44%), Postives = 457/469 (97.44%), Query Frame = 0

Query: 2    EKMMEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRCSFRRFSSPFSVSASASTARETDN 61
            EKMMEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLR SFRRFSSPFSVSASASTARETDN
Sbjct: 1105 EKMMEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRSSFRRFSSPFSVSASASTARETDN 1164

Query: 62   LHLGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHR 121
            LHLGSLSLG ITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHR
Sbjct: 1165 LHLGSLSLGRITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHR 1224

Query: 122  GMHFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDE- 181
            GMHFLSTKATDEYELARKKVASFINAR ASQIVFTRNATEAINLVAYSWGIPNLKLGDE 
Sbjct: 1225 GMHFLSTKATDEYELARKKVASFINARHASQIVFTRNATEAINLVAYSWGIPNLKLGDEV 1284

Query: 182  --------VIITVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKL 241
                    VIITVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKL
Sbjct: 1285 FLKLDLMQVIITVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKL 1344

Query: 242  VVTHHVSNVLASVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKM 301
            VVTHHVSNVLASVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKM
Sbjct: 1345 VVTHHVSNVLASVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKM 1404

Query: 302  CGPTSIGFLYGKSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLG 361
            CGPTSIGFLYGKSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLG
Sbjct: 1405 CGPTSIGFLYGKSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLG 1464

Query: 362  AAIDYLSGIGMQKIHSYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIH 421
            AAIDYLSGIGMQKIHSYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIH
Sbjct: 1465 AAIDYLSGIGMQKIHSYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIH 1524

Query: 422  PTDIASFLDQQHGVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDV 462
            PTDIASFLDQQHGVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDV
Sbjct: 1525 PTDIASFLDQQHGVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDV 1573

BLAST of Carg02669 vs. NCBI nr
Match: XP_022995078.1 (cysteine desulfurase 1, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 897.9 bits (2319), Expect = 3.8e-257
Identity = 450/458 (98.25%), Postives = 452/458 (98.69%), Query Frame = 0

Query: 4   MMEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRCSFRRFSSPFSVSASASTARETDNLH 63
           MMEGVALKLQFRNPTHCSSLFSST+RHSS  FLRCSFRR SSPFSVSASASTARETDNLH
Sbjct: 1   MMEGVALKLQFRNPTHCSSLFSSTLRHSSSPFLRCSFRRLSSPFSVSASASTARETDNLH 60

Query: 64  LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM 123
           LG LSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM
Sbjct: 61  LGPLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM 120

Query: 124 HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII 183
           HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII
Sbjct: 121 HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII 180

Query: 184 TVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSNVLA 243
           TVAEHHSAIVPWQIVAQKTGAVLK VNLGEHE+PNLKDFKEMFSTKTKLVVTHHVSNVLA
Sbjct: 181 TVAEHHSAIVPWQIVAQKTGAVLKIVNLGEHEVPNLKDFKEMFSTKTKLVVTHHVSNVLA 240

Query: 244 SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG 303
           SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG
Sbjct: 241 SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG 300

Query: 304 KSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM 363
           KSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM
Sbjct: 301 KSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM 360

Query: 364 QKIHSYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFLDQQ 423
           QKIHSYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFLDQQ
Sbjct: 361 QKIHSYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFLDQQ 420

Query: 424 HGVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDV 462
           HGVAIRSGHHCAQPLHRAL VTASARASLYFYNTKEDV
Sbjct: 421 HGVAIRSGHHCAQPLHRALSVTASARASLYFYNTKEDV 458

BLAST of Carg02669 vs. NCBI nr
Match: XP_023532532.1 (cysteine desulfurase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 882.5 bits (2279), Expect = 1.6e-252
Identity = 446/458 (97.38%), Postives = 448/458 (97.82%), Query Frame = 0

Query: 4   MMEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRCSFRRFSSPFSVSASASTARETDNLH 63
           MMEGVALKLQFR PT      SST+RHSS  FLRCSFRRFSSPFSVSASASTARETDNLH
Sbjct: 1   MMEGVALKLQFRIPT------SSTLRHSSSPFLRCSFRRFSSPFSVSASASTARETDNLH 60

Query: 64  LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM 123
           LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM
Sbjct: 61  LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM 120

Query: 124 HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII 183
           HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII
Sbjct: 121 HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII 180

Query: 184 TVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSNVLA 243
           TVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHE+PNLKDFKEMFSTKTKLVVTHHVSNVLA
Sbjct: 181 TVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEVPNLKDFKEMFSTKTKLVVTHHVSNVLA 240

Query: 244 SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG 303
           SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG
Sbjct: 241 SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG 300

Query: 304 KSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM 363
           KSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM
Sbjct: 301 KSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM 360

Query: 364 QKIHSYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFLDQQ 423
           QKIHSYEVELANYLYESLRAVPNIHIYGPAPT SVQRAALCSFNIENIHPTDIASFLDQQ
Sbjct: 361 QKIHSYEVELANYLYESLRAVPNIHIYGPAPTVSVQRAALCSFNIENIHPTDIASFLDQQ 420

Query: 424 HGVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDV 462
           HGVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDV
Sbjct: 421 HGVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDV 452

BLAST of Carg02669 vs. ExPASy Swiss-Prot
Match: Q93WX6 (Cysteine desulfurase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NFS2 PE=1 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 1.1e-190
Identity = 337/457 (73.74%), Postives = 382/457 (83.59%), Query Frame = 0

Query: 5   MEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRCSFRRFSSPFSVSASASTARETDNLHL 64
           MEGVA+KL    P+  +++   ++ H S S +RC     SS  SV ++A+ +  T +   
Sbjct: 1   MEGVAMKL----PSFPNAI---SIGHRSFSRVRC-----SSSLSVCSAAAASSATISTDS 60

Query: 65  GSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGMH 124
            S+SLGH  R DF ILHQEVNGSKLVYLD+AATSQKP AVL ALQNYYE YNSNVHRG+H
Sbjct: 61  ESVSLGHRVRKDFRILHQEVNGSKLVYLDSAATSQKPAAVLDALQNYYEFYNSNVHRGIH 120

Query: 125 FLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVIIT 184
           +LS KATDE+ELARKKVA FINA D+ +IVFTRNATEAINLVAYSWG+ NLK GDEVI+T
Sbjct: 121 YLSAKATDEFELARKKVARFINASDSREIVFTRNATEAINLVAYSWGLSNLKPGDEVILT 180

Query: 185 VAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSNVLAS 244
           VAEHHS IVPWQIV+QKTGAVLKFV L E E+P++   +E+ S KTKLV  HHVSNVLAS
Sbjct: 181 VAEHHSCIVPWQIVSQKTGAVLKFVTLNEDEVPDINKLRELISPKTKLVAVHHVSNVLAS 240

Query: 245 VLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYGK 304
            LPIEEIV WAH  GAKV+VDACQSVPHMVVDVQ L+ADFLVASSHKMCGPT IGFLYGK
Sbjct: 241 SLPIEEIVVWAHDVGAKVLVDACQSVPHMVVDVQKLNADFLVASSHKMCGPTGIGFLYGK 300

Query: 305 SELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGMQ 364
           S+LL +MPPFLGGGEMISDVFLDHS+YAEPPSRFEAGTPAIGEAI LGAA+DYLSGIGM 
Sbjct: 301 SDLLHSMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIALGAAVDYLSGIGMP 360

Query: 365 KIHSYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFLDQQH 424
           KIH YEVE+  YLYE L ++P++ IYGP P+ SV R ALCSFN+E +HPTD+A+FLDQQH
Sbjct: 361 KIHEYEVEIGKYLYEKLSSLPDVRIYGPRPSESVHRGALCSFNVEGLHPTDLATFLDQQH 420

Query: 425 GVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDV 462
           GVAIRSGHHCAQPLHR LGV ASARASLYFYNTK+DV
Sbjct: 421 GVAIRSGHHCAQPLHRYLGVNASARASLYFYNTKDDV 445

BLAST of Carg02669 vs. ExPASy Swiss-Prot
Match: Q55793 (Probable cysteine desulfurase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=csd PE=1 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 1.5e-144
Identity = 252/394 (63.96%), Postives = 300/394 (76.14%), Query Frame = 0

Query: 68  SLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGMHFLS 127
           SL    R DFPIL+QE+NG  LVYLDNAATSQKPRAVL+ L +YYE  N+NVHRG H LS
Sbjct: 8   SLAATVRQDFPILNQEINGHPLVYLDNAATSQKPRAVLEKLMHYYENDNANVHRGAHQLS 67

Query: 128 TKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVIITVAE 187
            +ATD YE  R KVA FINAR   +IV+TRNATEAINLVAYSWG+ NLK GDE+I TV E
Sbjct: 68  VRATDAYEAVRNKVAKFINARSPREIVYTRNATEAINLVAYSWGMNNLKAGDEIITTVME 127

Query: 188 HHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSNVLASVLP 247
           HHS +VPWQ+VA KTGAVLKFV L E E  +L+ FK + S KTKLV   H+SN L  V P
Sbjct: 128 HHSNLVPWQMVAAKTGAVLKFVQLDEQESFDLEHFKTLLSEKTKLVTVVHISNTLGCVNP 187

Query: 248 IEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYGKSEL 307
            EEI   AH+ GAKV+VDACQS PH  +DVQ++D D+LVAS HKMC PT IGFLYGK E+
Sbjct: 188 AEEIAQLAHQAGAKVLVDACQSAPHYPLDVQLIDCDWLVASGHKMCAPTGIGFLYGKEEI 247

Query: 308 LSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGMQKIH 367
           L AMPPF GGGEMI++VF DH +  E P +FEAGTPAI EAI LGAA+DYL+ +GM+ IH
Sbjct: 248 LEAMPPFFGGGEMIAEVFFDHFTTGELPHKFEAGTPAIAEAIALGAAVDYLTDLGMENIH 307

Query: 368 SYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFLDQQHGVA 427
           +YEVEL +YL++ L  +P + +YGP P     RAAL SFN+  +H +D+A+ +DQ  G+A
Sbjct: 308 NYEVELTHYLWQGLGQIPQLRLYGPNPKHG-DRAALASFNVAGLHASDVATMVDQD-GIA 367

Query: 428 IRSGHHCAQPLHRALGVTASARASLYFYNTKEDV 462
           IRSGHHC QPLHR    + SARASLYFYNTKE++
Sbjct: 368 IRSGHHCTQPLHRLFDASGSARASLYFYNTKEEI 399

BLAST of Carg02669 vs. ExPASy Swiss-Prot
Match: O32164 (Cysteine desulfurase SufS OS=Bacillus subtilis (strain 168) OX=224308 GN=sufS PE=1 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 1.5e-115
Identity = 210/388 (54.12%), Postives = 272/388 (70.10%), Query Frame = 0

Query: 74  RPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGMHFLSTKATDE 133
           R  FPILHQ+VNG  LVYLD+AATSQKPRAV++ L  YY  YNSNVHRG+H L T+ATD 
Sbjct: 7   REQFPILHQQVNGHDLVYLDSAATSQKPRAVIETLDKYYNQYNSNVHRGVHTLGTRATDG 66

Query: 134 YELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVIITVAEHHSAIV 193
           YE AR+KV  FINA+  ++I+FT+  T ++N+VA S+   NLK GDEV+IT  EHH+ I+
Sbjct: 67  YEGAREKVRKFINAKSMAEIIFTKGTTTSLNMVALSYARANLKPGDEVVITYMEHHANII 126

Query: 194 PWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSNVLASVLPIEEIVG 253
           PWQ   + TGA LK++ L E    +L+D +E  ++ TK+V   HVSNVL +V PI+E+  
Sbjct: 127 PWQQAVKATGATLKYIPLQEDGTISLEDVRETVTSNTKIVAVSHVSNVLGTVNPIKEMAK 186

Query: 254 WAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYGKSELLSAMPP 313
            AH  GA +VVD  QS PHM +DVQ LD DF   SSHKMCGPT +G LYGK  LL  M P
Sbjct: 187 IAHDNGAVIVVDGAQSTPHMKIDVQDLDCDFFALSSHKMCGPTGVGVLYGKKALLENMEP 246

Query: 314 FLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGMQKIHSYEVEL 373
              GGEMI  V L  S++ E P +FEAGTP I  AIGLGAAID+L  IG+ +I  +E +L
Sbjct: 247 AEFGGEMIDFVGLYESTWKELPWKFEAGTPIIAGAIGLGAAIDFLEEIGLDEISRHEHKL 306

Query: 374 ANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFLDQQHGVAIRSGHH 433
           A Y  E  R +  + +YGP      +RA L +FN++++HP D+A+ LD + G+A+R+GHH
Sbjct: 307 AAYALERFRQLDGVTVYGPE-----ERAGLVTFNLDDVHPHDVATVLDAE-GIAVRAGHH 366

Query: 434 CAQPLHRALGVTASARASLYFYNTKEDV 462
           CAQPL + L VTA+ARAS Y YNT+E++
Sbjct: 367 CAQPLMKWLDVTATARASFYLYNTEEEI 388

BLAST of Carg02669 vs. ExPASy Swiss-Prot
Match: Q9K7A0 (Probable cysteine desulfurase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=csd PE=3 SV=2)

HSP 1 Score: 405.2 bits (1040), Expect = 1.0e-111
Identity = 211/391 (53.96%), Postives = 266/391 (68.03%), Query Frame = 0

Query: 71  HITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGMHFLSTKA 130
           H  +  FP+L QEVNGS LVYLD+AATSQKP AV++AL +YY  YNSNVHRG+H L T A
Sbjct: 4   HEIKKLFPVLDQEVNGSPLVYLDSAATSQKPIAVIEALDDYYRRYNSNVHRGVHTLGTLA 63

Query: 131 TDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVIITVAEHHS 190
           TD YE AR+K+  FI+A    +I+FTR  T AINLVA S+G  NL  GDE++IT  EHHS
Sbjct: 64  TDGYEGAREKIRRFIHASSTEEIIFTRGTTTAINLVAASYGRANLGEGDEIVITPMEHHS 123

Query: 191 AIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSNVLASVLPIEE 250
            I+PWQ VA+ TGA L ++ L +     ++D ++  S KTK+V   HVSNVL ++ P++E
Sbjct: 124 NIIPWQQVAKATGATLTYLPLQKDGTIKIEDVEKTISEKTKIVAIMHVSNVLGTINPVKE 183

Query: 251 IVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYGKSELLSA 310
           I   AHR GA ++VD  QS PHM +DVQ L  DF   S HKM GPT IG LYGK   L  
Sbjct: 184 IAEIAHRHGAIMLVDGAQSAPHMKIDVQELGCDFFAFSGHKMAGPTGIGVLYGKKAHLEK 243

Query: 311 MPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGMQKIHSYE 370
           M P   GGEMI  V L  S++ E P +FE GTP I  AIGLGAAID+L  IG+ +I  +E
Sbjct: 244 MEPVEFGGEMIDFVGLYDSTWKELPWKFEGGTPIIAGAIGLGAAIDFLEDIGLDEIEKHE 303

Query: 371 VELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFLDQQHGVAIRS 430
            ELA Y  + L  +  + +YGP      +RA L +FNIE++HP D+A+ LD   G+A+R+
Sbjct: 304 HELAQYALDRLSELEGMTVYGPQ-----KRAGLVTFNIEDVHPHDVATVLDAD-GIAVRA 363

Query: 431 GHHCAQPLHRALGVTASARASLYFYNTKEDV 462
           GHHCAQPL + L VTA+ARAS Y YNTKED+
Sbjct: 364 GHHCAQPLMKWLDVTATARASFYLYNTKEDI 388

BLAST of Carg02669 vs. ExPASy Swiss-Prot
Match: P63518 (Probable cysteine desulfurase OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) OX=158878 GN=csd PE=3 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 7.5e-107
Identity = 193/386 (50.00%), Postives = 265/386 (68.65%), Query Frame = 0

Query: 76  DFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGMHFLSTKATDEYE 135
           DFPIL Q+VNG +L YLD+ ATSQ P  VL  L++YY+ YNSNVHRG+H L + ATD YE
Sbjct: 14  DFPILDQKVNGKRLAYLDSTATSQTPMQVLNVLEDYYKRYNSNVHRGVHTLGSLATDGYE 73

Query: 136 LARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVIITVAEHHSAIVPW 195
            AR+ V  FINA+   +I+FTR  T +INLVA+S+G  N++ GDE+++T  EHH+ IVPW
Sbjct: 74  NARETVRRFINAKYFEEIIFTRGTTASINLVAHSYGDANVEEGDEIVVTEMEHHANIVPW 133

Query: 196 QIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSNVLASVLPIEEIVGWA 255
           Q +A++  A LKF+ +      N++D K+  + KTK+V   H+SNVL ++  ++ I   A
Sbjct: 134 QQLAKRKNATLKFIPMTADGELNIEDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIA 193

Query: 256 HRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYGKSELLSAMPPFL 315
           H+ GA + VD  Q+ PHM +D+Q ++ADF   S HKM GPT IG L+GK ELL  M P  
Sbjct: 194 HQHGAIISVDGAQAAPHMKLDMQEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIE 253

Query: 316 GGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGMQKIHSYEVELAN 375
            GG+MI  V    +++A+ P++FEAGTP I +AIGL  AI YL  IG   IH YE EL  
Sbjct: 254 FGGDMIDFVSKYDATWADLPTKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTI 313

Query: 376 YLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFLDQQHGVAIRSGHHCA 435
           Y YE + A+  I IYGP      +RA + +FN++++HP D+A+ +D + GVA+R+GHHCA
Sbjct: 314 YAYEQMSAIEGIEIYGPPKD---RRAGVITFNLQDVHPHDVATAVDTE-GVAVRAGHHCA 373

Query: 436 QPLHRALGVTASARASLYFYNTKEDV 462
           QPL + L V+++ARAS Y YNTKED+
Sbjct: 374 QPLMKWLNVSSTARASFYIYNTKEDI 395

BLAST of Carg02669 vs. ExPASy TrEMBL
Match: A0A6J1H1F2 (Cysteine desulfurase OS=Cucurbita moschata OX=3662 GN=LOC111459267 PE=4 SV=1)

HSP 1 Score: 911.8 bits (2355), Expect = 1.2e-261
Identity = 457/458 (99.78%), Postives = 457/458 (99.78%), Query Frame = 0

Query: 4   MMEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRCSFRRFSSPFSVSASASTARETDNLH 63
           MMEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRCSFRRFSSPFSVSASASTARETDNLH
Sbjct: 1   MMEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRCSFRRFSSPFSVSASASTARETDNLH 60

Query: 64  LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM 123
           LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM
Sbjct: 61  LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM 120

Query: 124 HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII 183
           HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII
Sbjct: 121 HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII 180

Query: 184 TVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSNVLA 243
           TVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSNVLA
Sbjct: 181 TVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSNVLA 240

Query: 244 SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG 303
           SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG
Sbjct: 241 SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG 300

Query: 304 KSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM 363
           KSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM
Sbjct: 301 KSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM 360

Query: 364 QKIHSYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFLDQQ 423
           QKIHSYEVELANYLYESLRAVPNIHIYGPAPT SVQRAALCSFNIENIHPTDIASFLDQQ
Sbjct: 361 QKIHSYEVELANYLYESLRAVPNIHIYGPAPTGSVQRAALCSFNIENIHPTDIASFLDQQ 420

Query: 424 HGVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDV 462
           HGVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDV
Sbjct: 421 HGVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDV 458

BLAST of Carg02669 vs. ExPASy TrEMBL
Match: A0A6J1K6W1 (Cysteine desulfurase OS=Cucurbita maxima OX=3661 GN=LOC111490732 PE=4 SV=1)

HSP 1 Score: 897.9 bits (2319), Expect = 1.8e-257
Identity = 450/458 (98.25%), Postives = 452/458 (98.69%), Query Frame = 0

Query: 4   MMEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRCSFRRFSSPFSVSASASTARETDNLH 63
           MMEGVALKLQFRNPTHCSSLFSST+RHSS  FLRCSFRR SSPFSVSASASTARETDNLH
Sbjct: 1   MMEGVALKLQFRNPTHCSSLFSSTLRHSSSPFLRCSFRRLSSPFSVSASASTARETDNLH 60

Query: 64  LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM 123
           LG LSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM
Sbjct: 61  LGPLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM 120

Query: 124 HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII 183
           HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII
Sbjct: 121 HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII 180

Query: 184 TVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSNVLA 243
           TVAEHHSAIVPWQIVAQKTGAVLK VNLGEHE+PNLKDFKEMFSTKTKLVVTHHVSNVLA
Sbjct: 181 TVAEHHSAIVPWQIVAQKTGAVLKIVNLGEHEVPNLKDFKEMFSTKTKLVVTHHVSNVLA 240

Query: 244 SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG 303
           SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG
Sbjct: 241 SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG 300

Query: 304 KSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM 363
           KSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM
Sbjct: 301 KSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM 360

Query: 364 QKIHSYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFLDQQ 423
           QKIHSYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFLDQQ
Sbjct: 361 QKIHSYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFLDQQ 420

Query: 424 HGVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDV 462
           HGVAIRSGHHCAQPLHRAL VTASARASLYFYNTKEDV
Sbjct: 421 HGVAIRSGHHCAQPLHRALSVTASARASLYFYNTKEDV 458

BLAST of Carg02669 vs. ExPASy TrEMBL
Match: A0A6J1H1S7 (Cysteine desulfurase OS=Cucurbita moschata OX=3662 GN=LOC111459267 PE=4 SV=1)

HSP 1 Score: 866.3 bits (2237), Expect = 5.9e-248
Identity = 440/458 (96.07%), Postives = 440/458 (96.07%), Query Frame = 0

Query: 4   MMEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRCSFRRFSSPFSVSASASTARETDNLH 63
           MMEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRCSFRRFSSPFSVSASASTARETDNLH
Sbjct: 1   MMEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRCSFRRFSSPFSVSASASTARETDNLH 60

Query: 64  LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM 123
           LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM
Sbjct: 61  LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM 120

Query: 124 HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII 183
           HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII
Sbjct: 121 HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII 180

Query: 184 TVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSNVLA 243
           TVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSNVLA
Sbjct: 181 TVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSNVLA 240

Query: 244 SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG 303
           SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG
Sbjct: 241 SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG 300

Query: 304 KSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM 363
           KSELLSAMPPFL                 EPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM
Sbjct: 301 KSELLSAMPPFL-----------------EPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM 360

Query: 364 QKIHSYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFLDQQ 423
           QKIHSYEVELANYLYESLRAVPNIHIYGPAPT SVQRAALCSFNIENIHPTDIASFLDQQ
Sbjct: 361 QKIHSYEVELANYLYESLRAVPNIHIYGPAPTGSVQRAALCSFNIENIHPTDIASFLDQQ 420

Query: 424 HGVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDV 462
           HGVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDV
Sbjct: 421 HGVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDV 441

BLAST of Carg02669 vs. ExPASy TrEMBL
Match: A0A6J1K4P0 (Cysteine desulfurase OS=Cucurbita maxima OX=3661 GN=LOC111490732 PE=4 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 8.8e-244
Identity = 433/458 (94.54%), Postives = 435/458 (94.98%), Query Frame = 0

Query: 4   MMEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRCSFRRFSSPFSVSASASTARETDNLH 63
           MMEGVALKLQFRNPTHCSSLFSST+RHSS  FLRCSFRR SSPFSVSASASTARETDNLH
Sbjct: 1   MMEGVALKLQFRNPTHCSSLFSSTLRHSSSPFLRCSFRRLSSPFSVSASASTARETDNLH 60

Query: 64  LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM 123
           LG LSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM
Sbjct: 61  LGPLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM 120

Query: 124 HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII 183
           HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII
Sbjct: 121 HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII 180

Query: 184 TVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSNVLA 243
           TVAEHHSAIVPWQIVAQKTGAVLK VNLGEHE+PNLKDFKEMFSTKTKLVVTHHVSNVLA
Sbjct: 181 TVAEHHSAIVPWQIVAQKTGAVLKIVNLGEHEVPNLKDFKEMFSTKTKLVVTHHVSNVLA 240

Query: 244 SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG 303
           SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG
Sbjct: 241 SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG 300

Query: 304 KSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM 363
           KSELLSAMPPFL                 EPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM
Sbjct: 301 KSELLSAMPPFL-----------------EPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM 360

Query: 364 QKIHSYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFLDQQ 423
           QKIHSYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFLDQQ
Sbjct: 361 QKIHSYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFLDQQ 420

Query: 424 HGVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDV 462
           HGVAIRSGHHCAQPLHRAL VTASARASLYFYNTKEDV
Sbjct: 421 HGVAIRSGHHCAQPLHRALSVTASARASLYFYNTKEDV 441

BLAST of Carg02669 vs. ExPASy TrEMBL
Match: A0A6J1H0D5 (Cysteine desulfurase OS=Cucurbita moschata OX=3662 GN=LOC111459267 PE=4 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 1.9e-238
Identity = 419/420 (99.76%), Postives = 419/420 (99.76%), Query Frame = 0

Query: 4   MMEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRCSFRRFSSPFSVSASASTARETDNLH 63
           MMEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRCSFRRFSSPFSVSASASTARETDNLH
Sbjct: 1   MMEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRCSFRRFSSPFSVSASASTARETDNLH 60

Query: 64  LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM 123
           LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM
Sbjct: 61  LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGM 120

Query: 124 HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII 183
           HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII
Sbjct: 121 HFLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVII 180

Query: 184 TVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSNVLA 243
           TVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSNVLA
Sbjct: 181 TVAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSNVLA 240

Query: 244 SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG 303
           SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG
Sbjct: 241 SVLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYG 300

Query: 304 KSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM 363
           KSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM
Sbjct: 301 KSELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM 360

Query: 364 QKIHSYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFLDQQ 423
           QKIHSYEVELANYLYESLRAVPNIHIYGPAPT SVQRAALCSFNIENIHPTDIASFLDQQ
Sbjct: 361 QKIHSYEVELANYLYESLRAVPNIHIYGPAPTGSVQRAALCSFNIENIHPTDIASFLDQQ 420

BLAST of Carg02669 vs. TAIR 10
Match: AT1G08490.1 (chloroplastic NIFS-like cysteine desulfurase )

HSP 1 Score: 667.5 bits (1721), Expect = 7.7e-192
Identity = 337/457 (73.74%), Postives = 382/457 (83.59%), Query Frame = 0

Query: 5   MEGVALKLQFRNPTHCSSLFSSTVRHSSPSFLRCSFRRFSSPFSVSASASTARETDNLHL 64
           MEGVA+KL    P+  +++   ++ H S S +RC     SS  SV ++A+ +  T +   
Sbjct: 1   MEGVAMKL----PSFPNAI---SIGHRSFSRVRC-----SSSLSVCSAAAASSATISTDS 60

Query: 65  GSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPRAVLKALQNYYEAYNSNVHRGMH 124
            S+SLGH  R DF ILHQEVNGSKLVYLD+AATSQKP AVL ALQNYYE YNSNVHRG+H
Sbjct: 61  ESVSLGHRVRKDFRILHQEVNGSKLVYLDSAATSQKPAAVLDALQNYYEFYNSNVHRGIH 120

Query: 125 FLSTKATDEYELARKKVASFINARDASQIVFTRNATEAINLVAYSWGIPNLKLGDEVIIT 184
           +LS KATDE+ELARKKVA FINA D+ +IVFTRNATEAINLVAYSWG+ NLK GDEVI+T
Sbjct: 121 YLSAKATDEFELARKKVARFINASDSREIVFTRNATEAINLVAYSWGLSNLKPGDEVILT 180

Query: 185 VAEHHSAIVPWQIVAQKTGAVLKFVNLGEHEIPNLKDFKEMFSTKTKLVVTHHVSNVLAS 244
           VAEHHS IVPWQIV+QKTGAVLKFV L E E+P++   +E+ S KTKLV  HHVSNVLAS
Sbjct: 181 VAEHHSCIVPWQIVSQKTGAVLKFVTLNEDEVPDINKLRELISPKTKLVAVHHVSNVLAS 240

Query: 245 VLPIEEIVGWAHRFGAKVVVDACQSVPHMVVDVQVLDADFLVASSHKMCGPTSIGFLYGK 304
            LPIEEIV WAH  GAKV+VDACQSVPHMVVDVQ L+ADFLVASSHKMCGPT IGFLYGK
Sbjct: 241 SLPIEEIVVWAHDVGAKVLVDACQSVPHMVVDVQKLNADFLVASSHKMCGPTGIGFLYGK 300

Query: 305 SELLSAMPPFLGGGEMISDVFLDHSSYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGMQ 364
           S+LL +MPPFLGGGEMISDVFLDHS+YAEPPSRFEAGTPAIGEAI LGAA+DYLSGIGM 
Sbjct: 301 SDLLHSMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIALGAAVDYLSGIGMP 360

Query: 365 KIHSYEVELANYLYESLRAVPNIHIYGPAPTASVQRAALCSFNIENIHPTDIASFLDQQH 424
           KIH YEVE+  YLYE L ++P++ IYGP P+ SV R ALCSFN+E +HPTD+A+FLDQQH
Sbjct: 361 KIHEYEVEIGKYLYEKLSSLPDVRIYGPRPSESVHRGALCSFNVEGLHPTDLATFLDQQH 420

Query: 425 GVAIRSGHHCAQPLHRALGVTASARASLYFYNTKEDV 462
           GVAIRSGHHCAQPLHR LGV ASARASLYFYNTK+DV
Sbjct: 421 GVAIRSGHHCAQPLHRYLGVNASARASLYFYNTKDDV 445