Carg02655 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg02655 vs. NCBI nr
Match: KAG7035916.1 (Serine/threonine-protein kinase Nek5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1533.5 bits (3969), Expect = 0.0e+00
Identity = 777/777 (100.00%), Postives = 777/777 (100.00%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300

Query: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360
           NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK
Sbjct: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360

Query: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420
           VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA
Sbjct: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420

Query: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480
           HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK
Sbjct: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480

Query: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540
           PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH
Sbjct: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540

Query: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDQLEETHPP 600
           QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDQLEETHPP
Sbjct: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDQLEETHPP 600

Query: 601 SCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSFEKLASSHLPAAFDDVIHVIRHSSFRVGS 660
           SCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSFEKLASSHLPAAFDDVIHVIRHSSFRVGS
Sbjct: 601 SCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSFEKLASSHLPAAFDDVIHVIRHSSFRVGS 660

Query: 661 EQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKSFRQRAEA 720
           EQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKSFRQRAEA
Sbjct: 661 EQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKSFRQRAEA 720

Query: 721 LEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDNARSS 778
           LEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDNARSS
Sbjct: 721 LEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDNARSS 777

BLAST of Carg02655 vs. NCBI nr
Match: KAG6605965.1 (Serine/threonine-protein kinase Nek5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1522.3 bits (3940), Expect = 0.0e+00
Identity = 775/784 (98.85%), Postives = 776/784 (98.98%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300

Query: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360
           NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK
Sbjct: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360

Query: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420
           VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA
Sbjct: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420

Query: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480
           HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK
Sbjct: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480

Query: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540
           PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH
Sbjct: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540

Query: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVL-------DQ 600
           QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVL       D+
Sbjct: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE 600

Query: 601 LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSFEKLASSHLPAAFDDVIHVIRH 660
           LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPS EKLASSHLPAAFDDVIHVIRH
Sbjct: 601 LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRH 660

Query: 661 SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS 720
           SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS
Sbjct: 661 SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS 720

Query: 721 FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN 778
           FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN
Sbjct: 721 FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN 780

BLAST of Carg02655 vs. NCBI nr
Match: XP_022957727.1 (serine/threonine-protein kinase Nek5-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1517.3 bits (3927), Expect = 0.0e+00
Identity = 773/784 (98.60%), Postives = 775/784 (98.85%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHS+FVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRR MAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRSMAESQNS 300

Query: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360
           NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK
Sbjct: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360

Query: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420
           VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA
Sbjct: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420

Query: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480
           HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK
Sbjct: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480

Query: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540
           PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH
Sbjct: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540

Query: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVL-------DQ 600
           QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVL       D+
Sbjct: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE 600

Query: 601 LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSFEKLASSHLPAAFDDVIHVIRH 660
           LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPS EKLASSHLPAAFDDVIHVIRH
Sbjct: 601 LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRH 660

Query: 661 SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS 720
           SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS
Sbjct: 661 SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS 720

Query: 721 FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN 778
           FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN
Sbjct: 721 FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN 780

BLAST of Carg02655 vs. NCBI nr
Match: XP_023532893.1 (serine/threonine-protein kinase Nek5-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1507.7 bits (3902), Expect = 0.0e+00
Identity = 768/784 (97.96%), Postives = 772/784 (98.47%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHS+FVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRR MAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRSMAESQNS 300

Query: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360
           NSSNSDRDSLISSDKNAPAMASNCDD+VTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK
Sbjct: 301 NSSNSDRDSLISSDKNAPAMASNCDDKVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360

Query: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420
           VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASN KA
Sbjct: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNQKA 420

Query: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480
           HAE+PPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK
Sbjct: 421 HAEVPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480

Query: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540
           PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPAR RQAELNVPDAVSSTE IH
Sbjct: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARTRQAELNVPDAVSSTERIH 540

Query: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVL-------DQ 600
           QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVL       D+
Sbjct: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE 600

Query: 601 LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSFEKLASSHLPAAFDDVIHVIRH 660
           LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPS EKLASSHLPAAFDDVIHVIRH
Sbjct: 601 LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRH 660

Query: 661 SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS 720
           SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS
Sbjct: 661 SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS 720

Query: 721 FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN 778
           FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN
Sbjct: 721 FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN 780

BLAST of Carg02655 vs. NCBI nr
Match: XP_022995026.1 (serine/threonine-protein kinase Nek5-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1445.6 bits (3741), Expect = 0.0e+00
Identity = 747/790 (94.56%), Postives = 757/790 (95.82%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDHYEIMEQIGRGAFGAAILV+HKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDHYEIMEQIGRGAFGAAILVYHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMA+LMKKSNG+HFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGLHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHS+FVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSI P VD  PDKRR TRDSRR MAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSITP-VDSLPDKRRTTRDSRRSMAESQNS 300

Query: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360
           NSSNSDRDSLISSDKNAPAMASNCDDRVTDND ASVDDHDGPELLVPSEEEQTHNTCSLK
Sbjct: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDKASVDDHDGPELLVPSEEEQTHNTCSLK 360

Query: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420
           VNDPMHSKPAQADHQS+TESRRPKTIRNIMLALKEGKARENYSPV G CVKSGVASN KA
Sbjct: 361 VNDPMHSKPAQADHQSHTESRRPKTIRNIMLALKEGKARENYSPVPGICVKSGVASNQKA 420

Query: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480
           HAE+PPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLP+VDPT KTK
Sbjct: 421 HAEVPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPMVDPTPKTK 480

Query: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540
            RYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPAR RQAE+NVPDAVSSTE IH
Sbjct: 481 QRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARTRQAEINVPDAVSSTERIH 540

Query: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVL-------DQ 600
           QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVL       D+
Sbjct: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE 600

Query: 601 LEETHPPSCSSSLQYEKGNP-GVDDKP-----LSDKVTSLNNPSFEKLASSHLPAAFDDV 660
           LEETHPPSCSSSLQYEKGNP GVDDKP     LSD+VTSLNNP  EKLASSHLPAAFDDV
Sbjct: 601 LEETHPPSCSSSLQYEKGNPGGVDDKPTHECSLSDEVTSLNNPPSEKLASSHLPAAFDDV 660

Query: 661 IHVIRHSSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARE 720
           IHVIRHSSFRVGSEQA TENLED SS KEMDGGSPPT PTKNSDSSEHTKPVEEEVAARE
Sbjct: 661 IHVIRHSSFRVGSEQALTENLEDHSSDKEMDGGSPPTVPTKNSDSSEHTKPVEEEVAARE 720

Query: 721 TLDVKSFRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKG 778
           TLDVKSFRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKG
Sbjct: 721 TLDVKSFRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKG 780

BLAST of Carg02655 vs. ExPASy Swiss-Prot
Match: Q0WPH8 (Serine/threonine-protein kinase Nek5 OS=Arabidopsis thaliana OX=3702 GN=NEK5 PE=1 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 1.6e-223
Identity = 487/946 (51.48%), Postives = 584/946 (61.73%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMD YE+MEQIGRGAFGAAILVHHK+E+KKYVLKKIRLARQTERCRRSAHQEM+LIA
Sbjct: 1   MESRMDQYELMEQIGRGAFGAAILVHHKAERKKYVLKKIRLARQTERCRRSAHQEMSLIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMA+LMKKSNG++FPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGVYFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHS++VLHRDLKCSNIFLTKD+DVRLGDFGLAKTLKADDL SSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNYVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLTSSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCC+YEMAA RPAFKAFDMAGLISK+NRSSIGPLP CYSPSLK LIK
Sbjct: 181 DIPYGFKSDIWSLGCCIYEMAAYRPAFKAFDMAGLISKVNRSSIGPLPPCYSPSLKALIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300
           GMLRKNPE+RPNASE+LKHPYLQPYVEQYRP+++     +P+K   +R+ RR MAESQNS
Sbjct: 241 GMLRKNPEYRPNASEILKHPYLQPYVEQYRPTLS-AASITPEKPLNSREGRRSMAESQNS 300

Query: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEE-QTHNTCSL 360
           NSS S++D+   SDKN   +  +  ++VT+ D   VDD D  + +  S E     +  + 
Sbjct: 301 NSS-SEKDNFYVSDKNIRYVVPSNGNKVTETDSGFVDDEDILDHVQQSAENGNLQSVSAT 360

Query: 361 KVNDPMHSKPAQADHQSNT-ESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNH 420
           K +     KP  +D + +  + R PKTIRNIM+ LKE KAREN SP+R N  +       
Sbjct: 361 KPDGHGILKPVHSDQRPDVIQPRHPKTIRNIMMVLKEEKARENGSPMRSNRSRPSSVPTQ 420

Query: 421 KAHAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSK 480
           K + E P K+ K       SK+NA TP  +P       S R      PKH +PV+D + K
Sbjct: 421 KNNVETPSKIPKLGDIAHSSKTNASTP--IPPSKLASDSARTPGSFPPKHHMPVIDSSPK 480

Query: 481 TKPRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPA-RARQAELNVPD-AVSST 540
            KPR D   P        ++ +S K RQRTPPT+ RR +  A ++RQ   ++ + A   T
Sbjct: 481 LKPRNDRISPSPAAKHEAEEAMSVKRRQRTPPTLPRRTSLIAHQSRQLGADISNMAAKET 540

Query: 541 EMIH-----QKETQ-----------HTTSEP------FSKGMQTDSSNSVSSSVSIQGFE 600
             +H     + ET             TT EP       +KGMQ++SSNS+SSS+S+Q FE
Sbjct: 541 AKLHPSVPSESETNSHQSRVHASPVSTTPEPKRTSVGSAKGMQSESSNSISSSLSMQAFE 600

Query: 601 ICDDAATPFTDIPE------------------------------------LVLDQLEET- 660
           +CDDA+TP+ D+ E                                    + L ++ ++ 
Sbjct: 601 LCDDASTPYIDMTEHTTPDDHRRSCHSEYSYSFPDISSEMMIRRDEHSTSMRLTEIPDSV 660

Query: 661 ---------HPP-----SCSSSL----------------QYEKGN------------PGV 720
                    H P     SC + L                Q++ G+            PG 
Sbjct: 661 SGVQNTIAHHQPEREQGSCPTVLKDDSPATLQSYEPNTSQHQHGDDKFTVKEFVSSVPGP 720

Query: 721 DDKPL-----------SDKVTSL--NNPSFEKLAS-SHLPAAFDDVIHVIRHSSFRVGSE 773
              PL           SD  TS+   N + EK  S SH     DDVIHVIRHSSFRVGS+
Sbjct: 721 APLPLHVEPSHQVNSHSDNKTSVMSQNSALEKNNSHSHPHPVVDDVIHVIRHSSFRVGSD 780

BLAST of Carg02655 vs. ExPASy Swiss-Prot
Match: Q94CU5 (Serine/threonine-protein kinase Nek5 OS=Oryza sativa subsp. japonica OX=39947 GN=NEK5 PE=2 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 8.6e-185
Identity = 430/948 (45.36%), Postives = 545/948 (57.49%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           M+SRMD YE+MEQIGRGAFGAAILV+HK+EKKKYVLKKIRLARQTERCR+SAHQEMALIA
Sbjct: 1   MDSRMDQYEVMEQIGRGAFGAAILVNHKTEKKKYVLKKIRLARQTERCRKSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           R+QHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMA+LMKK+NG +FPEEKL KWF QL LA
Sbjct: 61  RLQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKANGTYFPEEKLLKWFAQLALA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           V+YLHS+FVLHRDLKCSNIFLTKD+D+RLGDFGLAKTLKADDL SSVVGTPNYMCPELLA
Sbjct: 121 VDYLHSNFVLHRDLKCSNIFLTKDQDIRLGDFGLAKTLKADDLTSSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAA RPAFKAFDMAGLISKINRSSIGPLP CYSPS+K+LIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPPCYSPSMKSLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRP--SIAPTVDFSPDKRRPTRDSRRCMAESQ 300
            MLRK+PEHRP ASE+LK PYLQPYV QYRP   I+  +         +R S++ M+ SQ
Sbjct: 241 SMLRKSPEHRPTASEILKSPYLQPYVNQYRPFADISHPIHSLEKPITSSRSSQKSMSGSQ 300

Query: 301 NSNSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTH-NTC 360
            S+ S SD DS+ SS++N    +++ ++ + D + A   DH   +    S++ +++  T 
Sbjct: 301 CSSISGSDIDSIQSSERNTSGPSTSSNNTI-DTEGAEATDHVSVKNCSRSDDVKSNKETV 360

Query: 361 SLKVNDPMHSKPAQADHQSNTESRRPKTIRNIMLALK-EGKARENYSPVRGNCVKSGVAS 420
             ++     SK    D +   E ++PK I+ I+  L+ E K R+N SP+R + VK    S
Sbjct: 361 GPELERQDSSKSIHVDQRPRNEIKQPKIIKKILTTLREESKLRQNNSPIRASRVKLNSPS 420

Query: 421 NHKAHAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPT 480
           N +  ++      K    +  S  +++  +   AK   E  +R QA    KH  P+V+ +
Sbjct: 421 NREQLSDDS----KHSSDISSSSKSSEVTSRESAKVICEPVKRAQASPPLKHLSPIVEHS 480

Query: 481 SKTKPRYDNYPPLFHPVKPV---DDGLSSKSRQRTPPTVLRRPTFPAR---ARQAELNVP 540
            K K + D  P    P K      D    K + RTPP+  RR + P R     ++ L+  
Sbjct: 481 PKAKIKQDE-PLQPDPAKQAMEDVDAAVGKVKNRTPPSYSRRLSIPPRRPLGAESPLHAD 540

Query: 541 DAVSSTEMIHQK-------------------------------------ETQHTTSEPFS 600
              +  ++I ++                                       +  ++ P +
Sbjct: 541 TKRAHNKVIKERAKSPCRPVHGPDNDIIEPPGFPMAPPSPLGGVQMKVGNARAKSAPPRA 600

Query: 601 KGMQTDSSNSVSSSVS-IQGFEICD----DAATPFT-----DIPELVLDQLEETHPPSCS 660
             ++ DSS+  SS+++  +  E+ +    D++          IP+  + + + T  PS  
Sbjct: 601 VSIKEDSSDCSSSTIAYAENTELSEPSKQDSSAQLVSSCKCSIPDAAIQKHDLTAMPSSE 660

Query: 661 -SSLQYEKGNPGVDD-------KPLSD-----KVTSLNNP-------------------- 720
            ++  ++K     DD       +P SD      +   N P                    
Sbjct: 661 LNTTNFQKSMASNDDVCENLALEPSSDISEQVSIFKDNVPCSKISQSTANAIVQNDEDKF 720

Query: 721 -------------------------------SFEKLASSHLPAAFDDVIHVIRHSSFRVG 778
                                          S E+ +S HL    +DVIHVIRHSSFRV 
Sbjct: 721 TVQELLSSVADIAPFVSTKNFALEKGSPPIQSLERTSSPHLNPPIEDVIHVIRHSSFRVC 780

BLAST of Carg02655 vs. ExPASy Swiss-Prot
Match: Q60DG4 (Serine/threonine-protein kinase Nek4 OS=Oryza sativa subsp. japonica OX=39947 GN=NEK4 PE=2 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 8.3e-172
Identity = 420/947 (44.35%), Postives = 518/947 (54.70%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMD YEIMEQ+GRGAFGAAILV+HK E+KKYVLKKIRLARQTERCR+SAHQEMALIA
Sbjct: 1   MESRMDQYEIMEQVGRGAFGAAILVNHKIERKKYVLKKIRLARQTERCRKSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           R+QHPYIVEFKEAWVEKGCYVCIVTGYCEGGDM +LMKK NG +FPEEKL KWF QL+LA
Sbjct: 61  RLQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMDELMKKLNGTYFPEEKLLKWFAQLVLA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           V+YLHS++VLHRDLKCSNIFLTKD+D+RLGDFGLAKTLK DDL SSVVGTPNYMCPELL 
Sbjct: 121 VDYLHSNYVLHRDLKCSNIFLTKDQDIRLGDFGLAKTLKEDDLTSSVVGTPNYMCPELLT 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAA RPAFKAFDMAGLISKINRSSIGPLP+CYS S+KTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPACYSSSMKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRP-SIAPTVDFSPDKRRPT-RDSRRCMAESQ 300
            MLRK+PEHRP ASE+LK+PYLQPYV Q RP S APT    P+K   T R ++RC +ESQ
Sbjct: 241 SMLRKSPEHRPTASEILKNPYLQPYVNQCRPLSDAPTPIRMPEKPLSTSRSNQRCTSESQ 300

Query: 301 NSNSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHD----GPELLVPSEEEQTH 360
           +S+ S SD DS  SSD++    A + D ++  ND+ S+ D D      + + P +     
Sbjct: 301 SSSISCSDIDSTQSSDRSTSGGAPSTDSKL--NDIRSIQDADRADSDEKCVTPEDLRGNK 360

Query: 361 NTCSLKVNDPMHSKPAQADHQSNTESRRPKTIRNIMLALK-EGKARENYSPVRGNCVKSG 420
           N    ++     SK     H+   ES++PK I  IM  L+ E + REN SPV  + VK  
Sbjct: 361 NISGAELKRQDSSKSVHQHHRG--ESKQPKIIEKIMTTLREESRLRENNSPVSSSGVKLT 420

Query: 421 VASNHKAHAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVV 480
            A ++K  AE   +  +P   V  S    D  +      + E    +Q     K   P V
Sbjct: 421 SAVSNKNQAEQSSESSRPHSGVSYSSKFGDISSNGWTNTSDECVDPVQVPLQLKQLSPTV 480

Query: 481 DPTSKTKPRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELN-VPDA 540
           +   K K    + P     +       S  S+ ++ P+  RRP+ P R   A +  VP  
Sbjct: 481 EHCPKLKNSGSSTPEPAKQIAENGSSASGMSKTKSSPSSSRRPS-PQRQTVAGIPIVPFT 540

Query: 541 VSSTEMI---HQKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATP------- 600
           VS    I    +K        P +          +S+++S +  ++ +  A P       
Sbjct: 541 VSKRAHIKAESEKTPPRPAHSPNNSLHNLPPLIPISTNLSEENIKLGNSQAMPAPLEFVT 600

Query: 601 ------FTDIPELVLDQLEETHP-------------PSCSSSLQYEKGNPGV-------- 660
                  +     V+D +E+  P             P  +  +   KG  G+        
Sbjct: 601 AASKEDISFYSNSVVDCVEKAEPSEVFESNSPAYLTPPWTGPVLDAKGENGLIAIPCSEI 660

Query: 661 ------------DDKPLS------------------------------------DK---- 720
                       DD  LS                                    DK    
Sbjct: 661 HTGTLQKSMASNDDSSLSSPLDTFYLSFEQEFVCKDDSQSSKHGHSAVTLLSGEDKFTVQ 720

Query: 721 ------------VTSLNN---------PSFEKLASSHLPAAFDDVIHVIRHSSFRVGSEQ 778
                       V+S +N          SF+K + SH     D     IR +SF V  E 
Sbjct: 721 ELLASTPVISPFVSSTSNTLPEDKSSYQSFKKQSDSHSGPPVDVPAQTIRLNSFLVSDEW 780

BLAST of Carg02655 vs. ExPASy Swiss-Prot
Match: Q2QMH1 (Serine/threonine-protein kinase Nek2 OS=Oryza sativa subsp. japonica OX=39947 GN=NEK2 PE=2 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 7.1e-123
Identity = 302/775 (38.97%), Postives = 406/775 (52.39%), Query Frame = 0

Query: 5   MDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIARVQH 64
           MD YE++EQIG+GAFG+A+LV HK EKKKYVLKKIRLARQT+R RRSAHQEM LIA V++
Sbjct: 1   MDQYEVLEQIGKGAFGSALLVRHKLEKKKYVLKKIRLARQTDRTRRSAHQEMQLIATVRN 60

Query: 65  PYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLAVEYL 124
           P+IVE+K++WVEKGCYVCIV GYCEGGDMA+ +K++NG +F EEKLCKW  QLL+A++YL
Sbjct: 61  PFIVEYKDSWVEKGCYVCIVIGYCEGGDMAEAIKRANGTYFSEEKLCKWLVQLLMALDYL 120

Query: 125 HSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLADIPY 184
           H++ +LHRD+KCSNIF+ +D+ +RLGDFGLAK L +DDLASSVVGTP+YMCPELLADIPY
Sbjct: 121 HANHILHRDVKCSNIFIARDQSIRLGDFGLAKILTSDDLASSVVGTPSYMCPELLADIPY 180

Query: 185 GFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIKGMLR 244
           G KSDIWSLGCC+YEM A RPAFKAFDM  LI+KI +S + PLP+ YS + + LIK MLR
Sbjct: 181 GTKSDIWSLGCCIYEMTALRPAFKAFDMQALINKITKSIVSPLPTKYSGAFRGLIKSMLR 240

Query: 245 KNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNSNSSN 304
           K+PEHRP+A++LLKHP LQPYV Q +   +PT                            
Sbjct: 241 KSPEHRPSAAQLLKHPQLQPYVLQVQLKSSPT---------------------------- 300

Query: 305 SDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLKVNDP 364
                     +N   +  +  D+V              ++  PS+           V D 
Sbjct: 301 ----------RNILPIHQSLTDKV-------------KKMTFPSD-----------VVDS 360

Query: 365 MHSKPAQADHQSNTES---RRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKAH 424
              + A+ +   N  +    +P   RN + + +  K       V+G  V S  A +    
Sbjct: 361 ARRRMARRNSLGNERTVTFSKPSPERNSVSSTRSIKEYTTTQSVKGLSVDSSEAGD---- 420

Query: 425 AEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTKP 484
            E+  K I     +  + S   TP ++PAK             T ++QL         +P
Sbjct: 421 -EVTSKAI-----ITKTSSILRTPKSLPAK-----------TYTARNQL---------EP 480

Query: 485 RYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIHQ 544
              +Y   +    P           +T P  + RP     AR+A L              
Sbjct: 481 PKTSYNRTYRSELP----------SKTTPNKIARP-----ARRASL-------------- 540

Query: 545 KETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDQLEETHPPS 604
                    P S   +T +  S+S                        +L+QL+      
Sbjct: 541 ---------PLST-YETPTKRSIS------------------------ILEQLD------ 587

Query: 605 CSSSLQYEKGNPGVDDKPLSDKVTSLNNPSFEKLASSHLPAAFDDVIHVIRHSSFRVGSE 664
                     +P V          S+N P  +++A   L ++ D ++ +  H+    G  
Sbjct: 601 ----------SPDV----------SVNAPRIDRIAEFPLASSEDPLLPI--HNKLSPGHG 587

Query: 665 QAATENLEDRSSCKEM----------DGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDV 724
             +T    +RS  K+           D GS  +     + SS  +       + ++  D 
Sbjct: 661 SCSTPPFINRSITKDKCTIQVLRTDGDNGSDSSGRNATAASSRGSND-----SRQQRFDT 587

Query: 725 KSFRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLK 767
            SF+QRAEALE LLE SA+LLQ  R EELG++LKPFG +K SPRETAIWL KS K
Sbjct: 721 SSFQQRAEALEGLLEFSAQLLQQERYEELGILLKPFGPEKASPRETAIWLTKSFK 587

BLAST of Carg02655 vs. ExPASy Swiss-Prot
Match: A2ZMH2 (Serine/threonine-protein kinase Nek2 OS=Oryza sativa subsp. indica OX=39946 GN=NEK2 PE=3 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 1.2e-122
Identity = 302/775 (38.97%), Postives = 405/775 (52.26%), Query Frame = 0

Query: 5   MDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIARVQH 64
           MD YE++EQIG+GAFG+A+LV HK EKKKYVLKKIRLARQT+R RRSAHQEM LIA V++
Sbjct: 1   MDQYEVLEQIGKGAFGSALLVRHKLEKKKYVLKKIRLARQTDRTRRSAHQEMQLIATVRN 60

Query: 65  PYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLAVEYL 124
           P+IVE+K++WVEKGCYVCIV GYCEGGDMA+ +K++NG +F EEKLCKW  QLL+A++YL
Sbjct: 61  PFIVEYKDSWVEKGCYVCIVIGYCEGGDMAEAIKRANGTYFSEEKLCKWLVQLLMALDYL 120

Query: 125 HSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLADIPY 184
           H++ +LHRD+KCSNIF+ +D+ +RLGDFGLAK L +DDLASSVVGTP+YMCPELLADIPY
Sbjct: 121 HANHILHRDVKCSNIFIARDQSIRLGDFGLAKILTSDDLASSVVGTPSYMCPELLADIPY 180

Query: 185 GFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIKGMLR 244
           G KSDIWSLGCC+YEM A RPAFKAFDM  LI+KI +S + PLP+ YS + + LIK MLR
Sbjct: 181 GTKSDIWSLGCCIYEMTALRPAFKAFDMQALINKITKSIVSPLPTKYSGAFRGLIKSMLR 240

Query: 245 KNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNSNSSN 304
           K+PEHRP+A++LLKHP LQPYV Q +   +PT                            
Sbjct: 241 KSPEHRPSAAQLLKHPQLQPYVLQVQLKSSPT---------------------------- 300

Query: 305 SDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLKVNDP 364
                     +N   +  +  D+V              ++  PS+           V D 
Sbjct: 301 ----------RNILPIHQSLTDKV-------------KKMTFPSD-----------VVDS 360

Query: 365 MHSKPAQADHQSNTES---RRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKAH 424
              + A+ +   N  +    +P   RN + + +  K       V G  V S  A +    
Sbjct: 361 ARRRMARRNSLGNERTVTFSKPSPERNSVSSTRSIKEYTTTQSVEGLSVDSSEAGD---- 420

Query: 425 AEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTKP 484
            E+  K I     +  + S   TP ++PAK             T ++QL         +P
Sbjct: 421 -EVTSKAI-----ITKTSSILRTPKSLPAK-----------TYTARNQL---------EP 480

Query: 485 RYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIHQ 544
              +Y   +    P           +T P  + RP     AR+A L              
Sbjct: 481 PKTSYNRTYRSELP----------SKTTPNKIARP-----ARRASL-------------- 540

Query: 545 KETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDQLEETHPPS 604
                    P S   +T +  S+S                        +L+QL+      
Sbjct: 541 ---------PLST-YETPTKRSIS------------------------ILEQLD------ 587

Query: 605 CSSSLQYEKGNPGVDDKPLSDKVTSLNNPSFEKLASSHLPAAFDDVIHVIRHSSFRVGSE 664
                     +P V          S+N P  +++A   L ++ D ++ +  H+    G  
Sbjct: 601 ----------SPDV----------SVNAPRIDRIAEFPLASSEDPLLPI--HNKLSPGHG 587

Query: 665 QAATENLEDRSSCKEM----------DGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDV 724
             +T    +RS  K+           D GS  +     + SS  +       + ++  D 
Sbjct: 661 SCSTPPFINRSITKDKCTIQVLRTDGDNGSDSSGRNATAASSRGSND-----SRQQRFDT 587

Query: 725 KSFRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLK 767
            SF+QRAEALE LLE SA+LLQ  R EELG++LKPFG +K SPRETAIWL KS K
Sbjct: 721 SSFQQRAEALEGLLEFSAQLLQQERYEELGILLKPFGPEKASPRETAIWLTKSFK 587

BLAST of Carg02655 vs. ExPASy TrEMBL
Match: A0A6J1GZX8 (serine/threonine-protein kinase Nek5-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459186 PE=4 SV=1)

HSP 1 Score: 1517.3 bits (3927), Expect = 0.0e+00
Identity = 773/784 (98.60%), Postives = 775/784 (98.85%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHS+FVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRR MAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRSMAESQNS 300

Query: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360
           NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK
Sbjct: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360

Query: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420
           VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA
Sbjct: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420

Query: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480
           HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK
Sbjct: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480

Query: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540
           PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH
Sbjct: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540

Query: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVL-------DQ 600
           QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVL       D+
Sbjct: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE 600

Query: 601 LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSFEKLASSHLPAAFDDVIHVIRH 660
           LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPS EKLASSHLPAAFDDVIHVIRH
Sbjct: 601 LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRH 660

Query: 661 SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS 720
           SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS
Sbjct: 661 SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS 720

Query: 721 FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN 778
           FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN
Sbjct: 721 FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN 780

BLAST of Carg02655 vs. ExPASy TrEMBL
Match: A0A6J1K0V2 (serine/threonine-protein kinase Nek5-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490704 PE=4 SV=1)

HSP 1 Score: 1445.6 bits (3741), Expect = 0.0e+00
Identity = 747/790 (94.56%), Postives = 757/790 (95.82%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDHYEIMEQIGRGAFGAAILV+HKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDHYEIMEQIGRGAFGAAILVYHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMA+LMKKSNG+HFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGLHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHS+FVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSI P VD  PDKRR TRDSRR MAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSITP-VDSLPDKRRTTRDSRRSMAESQNS 300

Query: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360
           NSSNSDRDSLISSDKNAPAMASNCDDRVTDND ASVDDHDGPELLVPSEEEQTHNTCSLK
Sbjct: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDKASVDDHDGPELLVPSEEEQTHNTCSLK 360

Query: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420
           VNDPMHSKPAQADHQS+TESRRPKTIRNIMLALKEGKARENYSPV G CVKSGVASN KA
Sbjct: 361 VNDPMHSKPAQADHQSHTESRRPKTIRNIMLALKEGKARENYSPVPGICVKSGVASNQKA 420

Query: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480
           HAE+PPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLP+VDPT KTK
Sbjct: 421 HAEVPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPMVDPTPKTK 480

Query: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540
            RYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPAR RQAE+NVPDAVSSTE IH
Sbjct: 481 QRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARTRQAEINVPDAVSSTERIH 540

Query: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVL-------DQ 600
           QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVL       D+
Sbjct: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE 600

Query: 601 LEETHPPSCSSSLQYEKGNP-GVDDKP-----LSDKVTSLNNPSFEKLASSHLPAAFDDV 660
           LEETHPPSCSSSLQYEKGNP GVDDKP     LSD+VTSLNNP  EKLASSHLPAAFDDV
Sbjct: 601 LEETHPPSCSSSLQYEKGNPGGVDDKPTHECSLSDEVTSLNNPPSEKLASSHLPAAFDDV 660

Query: 661 IHVIRHSSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARE 720
           IHVIRHSSFRVGSEQA TENLED SS KEMDGGSPPT PTKNSDSSEHTKPVEEEVAARE
Sbjct: 661 IHVIRHSSFRVGSEQALTENLEDHSSDKEMDGGSPPTVPTKNSDSSEHTKPVEEEVAARE 720

Query: 721 TLDVKSFRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKG 778
           TLDVKSFRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKG
Sbjct: 721 TLDVKSFRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKG 780

BLAST of Carg02655 vs. ExPASy TrEMBL
Match: A0A6J1H131 (serine/threonine-protein kinase Nek5-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459186 PE=4 SV=1)

HSP 1 Score: 1322.4 bits (3421), Expect = 0.0e+00
Identity = 677/688 (98.40%), Postives = 679/688 (98.69%), Query Frame = 0

Query: 97  MKKSNGMHFPEEKLCKWFTQLLLAVEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAK 156
           MKKSNGMHFPEEKLCKWFTQLLLAVEYLHS+FVLHRDLKCSNIFLTKDRDVRLGDFGLAK
Sbjct: 1   MKKSNGMHFPEEKLCKWFTQLLLAVEYLHSNFVLHRDLKCSNIFLTKDRDVRLGDFGLAK 60

Query: 157 TLKADDLASSVVGTPNYMCPELLADIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLI 216
           TLKADDLASSVVGTPNYMCPELLADIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLI
Sbjct: 61  TLKADDLASSVVGTPNYMCPELLADIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLI 120

Query: 217 SKINRSSIGPLPSCYSPSLKTLIKGMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPT 276
           SKINRSSIGPLPSCYSPSLKTLIKGMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPT
Sbjct: 121 SKINRSSIGPLPSCYSPSLKTLIKGMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPT 180

Query: 277 VDFSPDKRRPTRDSRRCMAESQNSNSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASV 336
           VDFSPDKRRPTRDSRR MAESQNSNSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASV
Sbjct: 181 VDFSPDKRRPTRDSRRSMAESQNSNSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASV 240

Query: 337 DDHDGPELLVPSEEEQTHNTCSLKVNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEG 396
           DDHDGPELLVPSEEEQTHNTCSLKVNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEG
Sbjct: 241 DDHDGPELLVPSEEEQTHNTCSLKVNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEG 300

Query: 397 KARENYSPVRGNCVKSGVASNHKAHAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIES 456
           KARENYSPVRGNCVKSGVASNHKAHAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIES
Sbjct: 301 KARENYSPVRGNCVKSGVASNHKAHAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIES 360

Query: 457 SRRIQAVATPKHQLPVVDPTSKTKPRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRP 516
           SRRIQAVATPKHQLPVVDPTSKTKPRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRP
Sbjct: 361 SRRIQAVATPKHQLPVVDPTSKTKPRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRP 420

Query: 517 TFPARARQAELNVPDAVSSTEMIHQKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEIC 576
           TFPARARQAELNVPDAVSSTEMIHQKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEIC
Sbjct: 421 TFPARARQAELNVPDAVSSTEMIHQKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEIC 480

Query: 577 DDAATPFTDIPELVL-------DQLEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNN 636
           DDAATPFTDIPELVL       D+LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNN
Sbjct: 481 DDAATPFTDIPELVLDHQSIEHDELEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNN 540

Query: 637 PSFEKLASSHLPAAFDDVIHVIRHSSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKN 696
           PS EKLASSHLPAAFDDVIHVIRHSSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKN
Sbjct: 541 PSSEKLASSHLPAAFDDVIHVIRHSSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKN 600

Query: 697 SDSSEHTKPVEEEVAARETLDVKSFRQRAEALEELLELSAELLQHNRLEELGVVLKPFGK 756
           SDSSEHTKPVEEEVAARETLDVKSFRQRAEALEELLELSAELLQHNRLEELGVVLKPFGK
Sbjct: 601 SDSSEHTKPVEEEVAARETLDVKSFRQRAEALEELLELSAELLQHNRLEELGVVLKPFGK 660

Query: 757 DKVSPRETAIWLAKSLKGMKMEDNARSS 778
           DKVSPRETAIWLAKSLKGMKMEDNARSS
Sbjct: 661 DKVSPRETAIWLAKSLKGMKMEDNARSS 688

BLAST of Carg02655 vs. ExPASy TrEMBL
Match: A0A6J1JXL4 (serine/threonine-protein kinase Nek5-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490704 PE=4 SV=1)

HSP 1 Score: 1254.6 bits (3245), Expect = 0.0e+00
Identity = 653/694 (94.09%), Postives = 661/694 (95.24%), Query Frame = 0

Query: 97  MKKSNGMHFPEEKLCKWFTQLLLAVEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAK 156
           MKKSNG+HFPEEKLCKWFTQLLLAVEYLHS+FVLHRDLKCSNIFLTKDRDVRLGDFGLAK
Sbjct: 1   MKKSNGLHFPEEKLCKWFTQLLLAVEYLHSNFVLHRDLKCSNIFLTKDRDVRLGDFGLAK 60

Query: 157 TLKADDLASSVVGTPNYMCPELLADIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLI 216
           TLKADDLASSVVGTPNYMCPELLADIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLI
Sbjct: 61  TLKADDLASSVVGTPNYMCPELLADIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLI 120

Query: 217 SKINRSSIGPLPSCYSPSLKTLIKGMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPT 276
           SKINRSSIGPLPSCYSPSLKTLIKGMLRKNPEHRPNASELLKHPYLQPYVEQYRPSI P 
Sbjct: 121 SKINRSSIGPLPSCYSPSLKTLIKGMLRKNPEHRPNASELLKHPYLQPYVEQYRPSITP- 180

Query: 277 VDFSPDKRRPTRDSRRCMAESQNSNSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASV 336
           VD  PDKRR TRDSRR MAESQNSNSSNSDRDSLISSDKNAPAMASNCDDRVTDND ASV
Sbjct: 181 VDSLPDKRRTTRDSRRSMAESQNSNSSNSDRDSLISSDKNAPAMASNCDDRVTDNDKASV 240

Query: 337 DDHDGPELLVPSEEEQTHNTCSLKVNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEG 396
           DDHDGPELLVPSEEEQTHNTCSLKVNDPMHSKPAQADHQS+TESRRPKTIRNIMLALKEG
Sbjct: 241 DDHDGPELLVPSEEEQTHNTCSLKVNDPMHSKPAQADHQSHTESRRPKTIRNIMLALKEG 300

Query: 397 KARENYSPVRGNCVKSGVASNHKAHAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIES 456
           KARENYSPV G CVKSGVASN KAHAE+PPKVIKPPVAVPGSKSNADTPTTVPAKANIES
Sbjct: 301 KARENYSPVPGICVKSGVASNQKAHAEVPPKVIKPPVAVPGSKSNADTPTTVPAKANIES 360

Query: 457 SRRIQAVATPKHQLPVVDPTSKTKPRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRP 516
           SRRIQAVATPKHQLP+VDPT KTK RYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRP
Sbjct: 361 SRRIQAVATPKHQLPMVDPTPKTKQRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRP 420

Query: 517 TFPARARQAELNVPDAVSSTEMIHQKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEIC 576
           TFPAR RQAE+NVPDAVSSTE IHQKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEIC
Sbjct: 421 TFPARTRQAEINVPDAVSSTERIHQKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEIC 480

Query: 577 DDAATPFTDIPELVL-------DQLEETHPPSCSSSLQYEKGNP-GVDDKP-----LSDK 636
           DDAATPFTDIPELVL       D+LEETHPPSCSSSLQYEKGNP GVDDKP     LSD+
Sbjct: 481 DDAATPFTDIPELVLDHQSIEHDELEETHPPSCSSSLQYEKGNPGGVDDKPTHECSLSDE 540

Query: 637 VTSLNNPSFEKLASSHLPAAFDDVIHVIRHSSFRVGSEQAATENLEDRSSCKEMDGGSPP 696
           VTSLNNP  EKLASSHLPAAFDDVIHVIRHSSFRVGSEQA TENLED SS KEMDGGSPP
Sbjct: 541 VTSLNNPPSEKLASSHLPAAFDDVIHVIRHSSFRVGSEQALTENLEDHSSDKEMDGGSPP 600

Query: 697 TAPTKNSDSSEHTKPVEEEVAARETLDVKSFRQRAEALEELLELSAELLQHNRLEELGVV 756
           T PTKNSDSSEHTKPVEEEVAARETLDVKSFRQRAEALEELLELSAELLQHNRLEELGVV
Sbjct: 601 TVPTKNSDSSEHTKPVEEEVAARETLDVKSFRQRAEALEELLELSAELLQHNRLEELGVV 660

Query: 757 LKPFGKDKVSPRETAIWLAKSLKGMKMEDNARSS 778
           LKPFGKDKVSPRETAIWLAKSLKGMKMEDNARSS
Sbjct: 661 LKPFGKDKVSPRETAIWLAKSLKGMKMEDNARSS 693

BLAST of Carg02655 vs. ExPASy TrEMBL
Match: A0A6J1FSG2 (serine/threonine-protein kinase Nek5-like OS=Cucurbita moschata OX=3662 GN=LOC111448376 PE=4 SV=1)

HSP 1 Score: 1218.4 bits (3151), Expect = 0.0e+00
Identity = 673/956 (70.40%), Postives = 706/956 (73.85%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMD YEIMEQIGRGAFGAAILV+HKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMA+LMKKSNGMHFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHS+FVLHRDLKCSNIFLTKD+DVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNFVLHRDLKCSNIFLTKDKDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAA RPAFKAFDMAGLISKINRSS+GPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSMGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSI+P+VDF  DK R  RDSRR MAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSISPSVDFPSDKPRAARDSRRSMAESQNS 300

Query: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360
           NSSNSDRDSL SSDKN PA ASNCDD+VT +DMASVDDHDG E L+P E+EQTHN CSLK
Sbjct: 301 NSSNSDRDSLFSSDKNTPATASNCDDKVTVSDMASVDDHDGSETLLPGEDEQTHNMCSLK 360

Query: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420
           VNDPMHSK  Q DHQSNTESRRPKTIRNIMLALKEGKAREN+SPVRGN VKSGVASN KA
Sbjct: 361 VNDPMHSKTNQEDHQSNTESRRPKTIRNIMLALKEGKARENFSPVRGNRVKSGVASNQKA 420

Query: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480
           HAE+PPK IKPPVA PG KSN DT  TVP KANIESSRR+Q VATPKHQ+PVV PT KTK
Sbjct: 421 HAEVPPKFIKPPVAEPGFKSNPDTHITVPTKANIESSRRVQVVATPKHQVPVVVPTPKTK 480

Query: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540
            R+DN PPL  P+KPV+DGLSSKSRQRTPPT+LRRP+FPAR RQ    VPDAVSS E IH
Sbjct: 481 TRHDNSPPLCRPLKPVEDGLSSKSRQRTPPTLLRRPSFPARMRQVGQAVPDAVSSMERIH 540

Query: 541 Q----------------------KETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDD 600
           Q                      KETQH +SE  SKGMQTDSSNSVSSSVSIQGFEICDD
Sbjct: 541 QKETSPEEVQSSHQDHVVSMELTKETQHASSEHCSKGMQTDSSNSVSSSVSIQGFEICDD 600

Query: 601 AATPFT--------DIPELVLDQLEETHPPSCSSSLQYE---------KGNPGVDDKP-- 660
           AATPF         D   +  D++EETHPPSCS SLQYE         KGN GV DKP  
Sbjct: 601 AATPFADGVIDPVFDHQSIEHDEIEETHPPSCSPSLQYEMSEHNLLTDKGNHGVHDKPTE 660

Query: 661 ------------------------------------------------------------ 720
                                                                       
Sbjct: 661 CSLEAGVEKESPSVELDLPLKSEGRFVFKDDIPINIPSNRPNMVTEPNTISSHGDDKFTV 720

Query: 721 ---------------------------LSDKVTSLNNPSFEKLASSHLPAAFDDVIHVIR 778
                                      L DKVT+L+NPS EKLAS+HLPAAFDDVIHVIR
Sbjct: 721 RELLSSVAEPTATPLVATPSSSTHKCLLPDKVTTLHNPSAEKLASNHLPAAFDDVIHVIR 780

BLAST of Carg02655 vs. TAIR 10
Match: AT3G44200.1 (NIMA (never in mitosis, gene A)-related 6 )

HSP 1 Score: 777.3 bits (2006), Expect = 1.1e-224
Identity = 487/946 (51.48%), Postives = 584/946 (61.73%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMD YE+MEQIGRGAFGAAILVHHK+E+KKYVLKKIRLARQTERCRRSAHQEM+LIA
Sbjct: 1   MESRMDQYELMEQIGRGAFGAAILVHHKAERKKYVLKKIRLARQTERCRRSAHQEMSLIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMA+LMKKSNG++FPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGVYFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHS++VLHRDLKCSNIFLTKD+DVRLGDFGLAKTLKADDL SSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNYVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLTSSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCC+YEMAA RPAFKAFDMAGLISK+NRSSIGPLP CYSPSLK LIK
Sbjct: 181 DIPYGFKSDIWSLGCCIYEMAAYRPAFKAFDMAGLISKVNRSSIGPLPPCYSPSLKALIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300
           GMLRKNPE+RPNASE+LKHPYLQPYVEQYRP+++     +P+K   +R+ RR MAESQNS
Sbjct: 241 GMLRKNPEYRPNASEILKHPYLQPYVEQYRPTLS-AASITPEKPLNSREGRRSMAESQNS 300

Query: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEE-QTHNTCSL 360
           NSS S++D+   SDKN   +  +  ++VT+ D   VDD D  + +  S E     +  + 
Sbjct: 301 NSS-SEKDNFYVSDKNIRYVVPSNGNKVTETDSGFVDDEDILDHVQQSAENGNLQSVSAT 360

Query: 361 KVNDPMHSKPAQADHQSNT-ESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNH 420
           K +     KP  +D + +  + R PKTIRNIM+ LKE KAREN SP+R N  +       
Sbjct: 361 KPDGHGILKPVHSDQRPDVIQPRHPKTIRNIMMVLKEEKARENGSPMRSNRSRPSSVPTQ 420

Query: 421 KAHAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSK 480
           K + E P K+ K       SK+NA TP  +P       S R      PKH +PV+D + K
Sbjct: 421 KNNVETPSKIPKLGDIAHSSKTNASTP--IPPSKLASDSARTPGSFPPKHHMPVIDSSPK 480

Query: 481 TKPRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPA-RARQAELNVPD-AVSST 540
            KPR D   P        ++ +S K RQRTPPT+ RR +  A ++RQ   ++ + A   T
Sbjct: 481 LKPRNDRISPSPAAKHEAEEAMSVKRRQRTPPTLPRRTSLIAHQSRQLGADISNMAAKET 540

Query: 541 EMIH-----QKETQ-----------HTTSEP------FSKGMQTDSSNSVSSSVSIQGFE 600
             +H     + ET             TT EP       +KGMQ++SSNS+SSS+S+Q FE
Sbjct: 541 AKLHPSVPSESETNSHQSRVHASPVSTTPEPKRTSVGSAKGMQSESSNSISSSLSMQAFE 600

Query: 601 ICDDAATPFTDIPE------------------------------------LVLDQLEET- 660
           +CDDA+TP+ D+ E                                    + L ++ ++ 
Sbjct: 601 LCDDASTPYIDMTEHTTPDDHRRSCHSEYSYSFPDISSEMMIRRDEHSTSMRLTEIPDSV 660

Query: 661 ---------HPP-----SCSSSL----------------QYEKGN------------PGV 720
                    H P     SC + L                Q++ G+            PG 
Sbjct: 661 SGVQNTIAHHQPEREQGSCPTVLKDDSPATLQSYEPNTSQHQHGDDKFTVKEFVSSVPGP 720

Query: 721 DDKPL-----------SDKVTSL--NNPSFEKLAS-SHLPAAFDDVIHVIRHSSFRVGSE 773
              PL           SD  TS+   N + EK  S SH     DDVIHVIRHSSFRVGS+
Sbjct: 721 APLPLHVEPSHQVNSHSDNKTSVMSQNSALEKNNSHSHPHPVVDDVIHVIRHSSFRVGSD 780

BLAST of Carg02655 vs. TAIR 10
Match: AT3G04810.1 (NIMA-related kinase 2 )

HSP 1 Score: 433.3 bits (1113), Expect = 4.0e-121
Identity = 301/767 (39.24%), Postives = 392/767 (51.11%), Query Frame = 0

Query: 5   MDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIARVQH 64
           M++YE++EQIG+G+FG+A+LV HK EKK YVLKKIRLARQT R RRSAHQEM LI+++ +
Sbjct: 1   MENYEVLEQIGKGSFGSALLVRHKHEKKLYVLKKIRLARQTGRTRRSAHQEMELISKIHN 60

Query: 65  PYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLAVEYL 124
           P+IVE+K++WVEKGCYVCI+ GYC+GGDMA+ +KK+NG+HF EEKLCKW  Q+LLA+EYL
Sbjct: 61  PFIVEYKDSWVEKGCYVCIIIGYCKGGDMAEAIKKTNGVHFTEEKLCKWLVQILLALEYL 120

Query: 125 HSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLADIPY 184
           H++ +LHRD+KCSNIFLTKD+D+RLGDFGLAK L +DDLASSVVGTP+YMCPELLADIPY
Sbjct: 121 HANHILHRDVKCSNIFLTKDQDIRLGDFGLAKVLTSDDLASSVVGTPSYMCPELLADIPY 180

Query: 185 GFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIKGMLR 244
           G KSDIWSLGCCMYEM A +PAFKAFDM GLI++INRS + PLP+ YS + + L+K MLR
Sbjct: 181 GSKSDIWSLGCCMYEMTAMKPAFKAFDMQGLINRINRSIVPPLPAQYSAAFRGLVKSMLR 240

Query: 245 KNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNSNSSN 304
           KNPE RP+A+ELL+ P LQPY+++                                    
Sbjct: 241 KNPELRPSAAELLRQPLLQPYIQKIH---------------------------------- 300

Query: 305 SDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLKVNDP 364
                                                                 LKVNDP
Sbjct: 301 ------------------------------------------------------LKVNDP 360

Query: 365 -MHSKPAQ-ADHQSNTESRRPKTIRNIMLALKEGKARE-NYSPVRGNCVKSGVASNHKAH 424
             +  PAQ  + +S   +  P+  R        GK+     S  RGN ++  V+S     
Sbjct: 361 GSNVLPAQWPESESARRNSFPEQRRR-----PAGKSHSFGPSRFRGN-LEDSVSS----- 420

Query: 425 AEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTKP 484
                 + K   A    +   D  T       +   RR    +     +PV    S  +P
Sbjct: 421 ------IKKTVPAYLNRERQVDLSTDASGDGTV--VRRTSEASKSSRYVPVRASASPVRP 480

Query: 485 RYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQA-ELNVPDAVSSTEMIH 544
           R         PV       SS+ + R P  ++RR + P+  + A E+     +S T  +H
Sbjct: 481 RQPRSDLGQLPV-------SSQLKNRKPAALIRRASMPSSRKPAKEIKDSLYISKTSFLH 540

Query: 545 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDQLEETHPP 604
           Q                       S  VS+    I D    P     E     +      
Sbjct: 541 QIN---------------------SPDVSMNAPRI-DKIEFPLASYEEEPFVPVVRGKKK 597

Query: 605 SCSSSLQYE-KGNPGVDDKPLSDKVTSLNNPSFEKLASSHLPAAFDDVIHVIRHSSFRVG 664
             SS   Y     P +D     DK T     + E    + + A +++  +          
Sbjct: 601 KASSRGSYSPPPEPPLDCSITKDKFTLEPGQNRE---GAIMKAVYEEDAY---------- 597

Query: 665 SEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKSFRQRAE 724
                   LEDRS   + +     TA   +  SS            R+  D  S++QRAE
Sbjct: 661 --------LEDRSESSDQNA----TAGASSRASSG---------VRRQRFDPSSYQQRAE 597

Query: 725 ALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLK 767
           ALE LLE SA LLQ  R +EL V+L+PFG  KVSPRETAIWL+KS K
Sbjct: 721 ALEGLLEFSARLLQDERYDELNVLLRPFGPGKVSPRETAIWLSKSFK 597

BLAST of Carg02655 vs. TAIR 10
Match: AT3G04810.2 (NIMA-related kinase 2 )

HSP 1 Score: 433.3 bits (1113), Expect = 4.0e-121
Identity = 301/767 (39.24%), Postives = 392/767 (51.11%), Query Frame = 0

Query: 5   MDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIARVQH 64
           M++YE++EQIG+G+FG+A+LV HK EKK YVLKKIRLARQT R RRSAHQEM LI+++ +
Sbjct: 1   MENYEVLEQIGKGSFGSALLVRHKHEKKLYVLKKIRLARQTGRTRRSAHQEMELISKIHN 60

Query: 65  PYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLAVEYL 124
           P+IVE+K++WVEKGCYVCI+ GYC+GGDMA+ +KK+NG+HF EEKLCKW  Q+LLA+EYL
Sbjct: 61  PFIVEYKDSWVEKGCYVCIIIGYCKGGDMAEAIKKTNGVHFTEEKLCKWLVQILLALEYL 120

Query: 125 HSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLADIPY 184
           H++ +LHRD+KCSNIFLTKD+D+RLGDFGLAK L +DDLASSVVGTP+YMCPELLADIPY
Sbjct: 121 HANHILHRDVKCSNIFLTKDQDIRLGDFGLAKVLTSDDLASSVVGTPSYMCPELLADIPY 180

Query: 185 GFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIKGMLR 244
           G KSDIWSLGCCMYEM A +PAFKAFDM GLI++INRS + PLP+ YS + + L+K MLR
Sbjct: 181 GSKSDIWSLGCCMYEMTAMKPAFKAFDMQGLINRINRSIVPPLPAQYSAAFRGLVKSMLR 240

Query: 245 KNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNSNSSN 304
           KNPE RP+A+ELL+ P LQPY+++                                    
Sbjct: 241 KNPELRPSAAELLRQPLLQPYIQKIH---------------------------------- 300

Query: 305 SDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLKVNDP 364
                                                                 LKVNDP
Sbjct: 301 ------------------------------------------------------LKVNDP 360

Query: 365 -MHSKPAQ-ADHQSNTESRRPKTIRNIMLALKEGKARE-NYSPVRGNCVKSGVASNHKAH 424
             +  PAQ  + +S   +  P+  R        GK+     S  RGN ++  V+S     
Sbjct: 361 GSNVLPAQWPESESARRNSFPEQRRR-----PAGKSHSFGPSRFRGN-LEDSVSS----- 420

Query: 425 AEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTKP 484
                 + K   A    +   D  T       +   RR    +     +PV    S  +P
Sbjct: 421 ------IKKTVPAYLNRERQVDLSTDASGDGTV--VRRTSEASKSSRYVPVRASASPVRP 480

Query: 485 RYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQA-ELNVPDAVSSTEMIH 544
           R         PV       SS+ + R P  ++RR + P+  + A E+     +S T  +H
Sbjct: 481 RQPRSDLGQLPV-------SSQLKNRKPAALIRRASMPSSRKPAKEIKDSLYISKTSFLH 540

Query: 545 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDQLEETHPP 604
           Q                       S  VS+    I D    P     E     +      
Sbjct: 541 QIN---------------------SPDVSMNAPRI-DKIEFPLASYEEEPFVPVVRGKKK 597

Query: 605 SCSSSLQYE-KGNPGVDDKPLSDKVTSLNNPSFEKLASSHLPAAFDDVIHVIRHSSFRVG 664
             SS   Y     P +D     DK T     + E    + + A +++  +          
Sbjct: 601 KASSRGSYSPPPEPPLDCSITKDKFTLEPGQNRE---GAIMKAVYEEDAY---------- 597

Query: 665 SEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKSFRQRAE 724
                   LEDRS   + +     TA   +  SS            R+  D  S++QRAE
Sbjct: 661 --------LEDRSESSDQNA----TAGASSRASSG---------VRRQRFDPSSYQQRAE 597

Query: 725 ALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLK 767
           ALE LLE SA LLQ  R +EL V+L+PFG  KVSPRETAIWL+KS K
Sbjct: 721 ALEGLLEFSARLLQDERYDELNVLLRPFGPGKVSPRETAIWLSKSFK 597

BLAST of Carg02655 vs. TAIR 10
Match: AT5G28290.1 (NIMA-related kinase 3 )

HSP 1 Score: 429.5 bits (1103), Expect = 5.8e-120
Identity = 302/763 (39.58%), Postives = 381/763 (49.93%), Query Frame = 0

Query: 5   MDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIARVQH 64
           M+HYE++EQIG+G+FG+A+LV HK EKK YVLKKIRLARQT R RRSAHQEM LI+++++
Sbjct: 1   MEHYEVLEQIGKGSFGSALLVRHKHEKKLYVLKKIRLARQTGRTRRSAHQEMELISKIRN 60

Query: 65  PYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLAVEYL 124
           P+IVE+K++WVEKGCYVCIV GYC+GGDMA+ +KK+NG+ F EEKLCKW  QLL+A+EYL
Sbjct: 61  PFIVEYKDSWVEKGCYVCIVIGYCKGGDMAEAIKKANGVEFSEEKLCKWLVQLLMALEYL 120

Query: 125 HSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLADIPY 184
           H+S +LHRD+KCSNIFLTKD+D+RLGDFGLAK L +DDLASSVVGTP+YMCPELLADIPY
Sbjct: 121 HASHILHRDVKCSNIFLTKDQDIRLGDFGLAKILTSDDLASSVVGTPSYMCPELLADIPY 180

Query: 185 GFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIKGMLR 244
           G KSDIWSLGCCMYEM A +PAFKAFDM GLI++INRS + PLP+ YS + ++L+K MLR
Sbjct: 181 GSKSDIWSLGCCMYEMTALKPAFKAFDMQGLINRINRSIVAPLPAQYSTAFRSLVKSMLR 240

Query: 245 KNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNSNSSN 304
           KNPE RP+AS+LL+ P LQPYV++    +   + F      P+   RR     Q   +S 
Sbjct: 241 KNPELRPSASDLLRQPLLQPYVQK----VLLKLSFREHDTLPSESERRSSYPQQRKRTSG 300

Query: 305 SDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEE-QTHNTCSLKVND 364
                   S    P+                VD  D    + P       H    L  ND
Sbjct: 301 -------KSVSFGPS-------------RFGVDQEDSVSSVKPVHTYLHRHRPVDLSAND 360

Query: 365 PMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKAHAE 424
                       S    RRP     +  + K       Y PVR N  KSG          
Sbjct: 361 -----------TSRVVVRRPAVSSVVSNSSK-------YVPVRSNQPKSG---------- 420

Query: 425 MPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTKPRY 484
               ++KP                              AV T +  LP+       KP  
Sbjct: 421 ---GLLKP------------------------------AVVTRRASLPI-----SQKPAK 480

Query: 485 DNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIHQKE 544
                L+HP   +   L+S       P +  R  FP              S  EM     
Sbjct: 481 GTKDSLYHPNIGILHQLNSPDVSVNSPRI-DRIKFP------------LASYEEM----- 540

Query: 545 TQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDQLEETHPPSCS 604
                  PF   ++    +S  S                ++  PE  LD         CS
Sbjct: 541 -------PFIPVVRKKKGSSRGS----------------YSPPPEPPLD---------CS 558

Query: 605 SSLQYEKGNPGVDDKPLSDKVTSLNNPSFEKLASSHLPAAFDDVIHVIRHSSFRVGSEQA 664
                                                          I    F +  E+ 
Sbjct: 601 -----------------------------------------------ITKDKFTLEPERE 558

Query: 665 ATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKSFRQRAEALEE 724
              +L D+++          TA   +  SS  ++        R+  D  S+RQRAEALE 
Sbjct: 661 TKSDLSDQNA----------TAGASSRASSGASR--------RQRFDPSSYRQRAEALEG 558

Query: 725 LLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLK 767
           LLE SA LL   R +EL V+LKPFG  KVSPRETAIWL+KS K
Sbjct: 721 LLEFSARLLIDERYDELNVLLKPFGPGKVSPRETAIWLSKSFK 558

BLAST of Carg02655 vs. TAIR 10
Match: AT1G54510.1 (NIMA-related serine/threonine kinase 1 )

HSP 1 Score: 427.2 bits (1097), Expect = 2.9e-119
Identity = 303/764 (39.66%), Postives = 412/764 (53.93%), Query Frame = 0

Query: 5   MDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIARVQH 64
           M+ YE +EQIG+G+FG+A+LV HK EKKKYVLKKIRLARQT+R RRSAHQEM LI++++H
Sbjct: 1   MEQYEFLEQIGKGSFGSALLVRHKHEKKKYVLKKIRLARQTQRTRRSAHQEMELISKMRH 60

Query: 65  PYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLAVEYL 124
           P+IVE+K++WVEK CYVCIV GYCEGGDMA  +KKSNG+HF EEKLCKW  QLL+ +EYL
Sbjct: 61  PFIVEYKDSWVEKACYVCIVIGYCEGGDMAQAIKKSNGVHFQEEKLCKWLVQLLMGLEYL 120

Query: 125 HSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLADIPY 184
           HS+ +LHRD+KCSNIFLTK++D+RLGDFGLAK L +DDL SSVVGTP+YMCPELLADIPY
Sbjct: 121 HSNHILHRDVKCSNIFLTKEQDIRLGDFGLAKILTSDDLTSSVVGTPSYMCPELLADIPY 180

Query: 185 GFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIKGMLR 244
           G KSDIWSLGCC+YEMA  +PAFKAFDM  LI+KIN++ + PLP+ YS   + L+K MLR
Sbjct: 181 GSKSDIWSLGCCIYEMAYLKPAFKAFDMQALINKINKTIVSPLPAKYSGPFRGLVKSMLR 240

Query: 245 KNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNSNSSN 304
           KNPE RP+AS+LL+HP+LQPYV   +  +      +     P+  S+R M ++  S    
Sbjct: 241 KNPEVRPSASDLLRHPHLQPYVLDVKLRLNNLRRKTLPPELPS--SKRIMKKAHFSE--- 300

Query: 305 SDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLKVNDP 364
                                                P +  P+  E+ H +     ND 
Sbjct: 301 -------------------------------------PAVTCPAFGERQHRSL---WNDR 360

Query: 365 MHSKPAQADHQSNTE--SRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKAHA 424
             +  A+ D  S+ +  SRR   I ++ +           S  +G  +   V+S+     
Sbjct: 361 ALNPEAEEDTASSIKCISRR---ISDLSIE----------SSSKGTLICKQVSSS----- 420

Query: 425 EMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTKPR 484
               KV K P+                AK+++ +SRRI                 +T  R
Sbjct: 421 --ACKVSKYPL----------------AKSSV-TSRRIM----------------ETGRR 480

Query: 485 YDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIHQK 544
            D+     HPV     G +SK                         +P A          
Sbjct: 481 SDH----LHPVS--GGGTTSKI------------------------IPSA---------- 540

Query: 545 ETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDQLEETHPPSC 604
                TS P +K     ++  V++   I G  I  +  +P   I E  +D++      + 
Sbjct: 541 ---RRTSLPLTK---RATNQEVAAYNPIVG--ILQNVKSPEYSINEPQVDKI------AI 589

Query: 605 SSSLQYEKGNPGVDDKPLSDKVTSLNNPSFEKLASSHLPAAFDDVIHVIRHSSFRVGSEQ 664
                YE+    +   P+  K +S ++   ++  +        D   V  H++++ G + 
Sbjct: 601 FPLAPYEQ---DIFFTPMQRKTSSKSSSVSDRSITK-------DKCTVQTHTTWQ-GIQL 589

Query: 665 AATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKSFRQRAEALE 724
              +N+ D SS  + +  +  ++ T +S S             R   D  S+RQRA+ALE
Sbjct: 661 NMVDNISDGSSSSDQNATAGASSHTTSSSS------------RRCRFDPSSYRQRADALE 589

Query: 725 ELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLK 767
            LLE SA LLQ  R +EL V+LKPFG  KVSPRETAIW+AKSLK
Sbjct: 721 GLLEFSARLLQEGRYDELNVLLKPFGPGKVSPRETAIWIAKSLK 589