Carg02642 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg02642
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	DNA polymerase epsilon subunit 3
Location	Carg_Chr02: 7650403 .. 7653096 (+)
RNA-Seq Expression	Carg02642
Synteny	Carg02642

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCGAGAACGATGAATGTGGAACTGCAGAGGCCGCCTCTGAGAGAGATCAATCTTTAAGCTGAAGAGCCAAAGAAGAACACTCTTGAGGCATCGTTGAGGTAGAGAGTAGAGGAAGATGGAGAAAAAAGTTGGGGCTGAGATCGAAGAGCTTCCGAAGACTATAGTTCGCCGAGTGGTTAAAGAGAAGCTTTCTCAGTGCTCTCGGAACCAGGATATTTCCGTCAACAAAGATTCGCTTCTAGCCTTTTGCGAGAGCGCTCGGATCTTCATTCATTACCTCTCCGCAACGTAAGTACGGCATCCTGCCTTTTTTTTTTGAATCCTGAATGAAATTCTATCGATTCTGATGTTCGAAATTAGGGTTCGTTGTTTCTCTTCTGTGTGCCTGAAGAACGAAAGCGAAAGAGATTAAAGTGATGAATCTCTTGTTGTTATGGCGCTAGGATTAAATAAATTGTTGTTATGTATTAGTTAATTTTTGCGTTCGTATTGACTGGTTGATCTCTAAGCAGGTCGAATGATATATGCAAGGAATCGAAGAGGCAGACGATTAAGGCGGAAGATGTATTGAAAGCTCTAGAAGATATGGAATTTCCCGAGTTGGTTAGGCCTCTTAAGGCTTCCCTCAATGGTGCGTTAGGTTCTAACCACTCAGTTTACCTTTTTGTTTTTTTTCTCCGGAAAATCATTACTCATTGGAGCATGAAATTGCACGATTAATTGGATAACTACGTTTTGATAGTTGTAACTTGCTGAACATTCTTTAAAAGCCGAAAGATGATCTAATGGTTCTCTTTTATTTGGTTGCTAGCATGATCTTGTTTTTATGCTCTTGCATGATTTCGTTGCCTAAAATGTCTGGTATTCTAGACTTTCAATCTCGATTTTGATGAATTTCGTTAACACTTCAGGAGCGTGAAATATTGCACGGCTATTGTGCAAAGAGGCCGTTAACATTATAAGAAAATTCTTATCAGTTCCTCAAACATCCAAGACGAGTCGATTTGGCATCATGATAGGATAGAGTTCTCATGTGAAAAGTGGATACAAGGTACAAAATGCAAGTCTGAAGGACTGAGCCGTTAGTTTCTGTAGTTGAATTGTTCATGTAGTATAAGAAGACTGTAGGAATTCATCTCAATCTCCTTGCCTAGTTTAGGGAAGAAATCAATTGCTCCTTTGTTTTAGAAAGAATTCCTATTCATTGAGTGGGGTATTGGTGAAAGTTTTTGCTGCATGACTCTTTCTACAATAGAACTCTTGTGACAAAAGAAAAGCGGATTTTTCGAAGTTCCCTCTTTAATTCCTATGAACTCTTGCTTGACATAAAAAATGTATAATTAATCATTTACCTGAGGTTCTACTTGATTGATCTTCAAGTTGGGGGAATATAATTTAGAGTTCAAGTAACCCATGCATGCCTTTTATCAAAAATTTATTATTCGATTAGATTGAATAATCTTTCATTAAATAAATATTCAAGTGCTTATGAATCCTTGGAGGTTTTTGTTGGATCTATCTTTAGTTATGTTGTCTATTTGTATTAGTAGAAGTATATACTTGGACCATTTTGAAAATTAGGTAAAGTGAGCAAGATTTCTATTTACTGGTGTGTAACCAGGCTCTACTAGTTATATTTCATCTGTCGTTGGACATGTGAGGTTTTATATGGAATCTGATTATACTTTATATTCTTGATATGAAATGATTGTATGAGATCCTTTATTAATGATTTATATAGTGGGTTATGAAATTTTTATACTGCAGTACGAGTTCAATGTGTAGATACTTTCTTTTCTCTTGATTATTAGGCAGGATGGGTAATTGTCATGCTACAGTATGCCTACTCTTTTCAGATAATATATGAAACTAAGCTATTTTCTTATCTTGGACGACGAGTAATGGGGTCTTGAGCTAAGCCTTGGATGTTTTAGTGCCATAATGCCTTCCAAATCCTCCTAGCCATTATCCGACTTCTACGTCATTTGATATCTGTGGTTAATTTAATGACAAAAGAAATGTTGAATTTATTGTACGAAGAAAGCACTTTTACTTTCCGCAGGCTATGATTTTCTATGGCATTTTCAAACTTTTATCCAGGATGAGTATGAAAGAAAGCTAGTTGAACCTTTTTAATGATTTATTATGCAACCTTGGCTTGGCTGAATGTAGAATTCAGAAGTAAGAATGCTGGAAAGAAGGCAGCTGCGTCGAGGATAAAAGAAGCGAAAAAGAGGAAGATAGAAGAAGAGCCTACAGTTGAAGGTACTGATGGTGAAAGCAAAGATGATGTCGAAAATGACAATGACGATAACGATAAAGATGAAGATGAAGTTGAAGATGAAGATGAAGTTGAGAATGGTGGCAGTGATGACTGAGAAACATGATGTATTTCCCTAATTAGGTACTTAAAATGAGGTTATTCCTCACTCTGTACGCATAAGATGGTTTACAAGTCTTCTAGGAATATATTTTTCTTTTTTCTGAGACTAATTTAGGTTGTATATTGTGAAGCGGCAGATGCACTTTTTGGCAAGTTCTGTATCTCTGTCCACATGGTAGTGAGAGAGGCTCCCATACCTTATTAAATCCATTTGTTTTCTGGGTTTTTCCCCATAGTTTTTCGAATGCCAGACATGAAGGACATGTTTGTTTCTTAGTTTCTAACGTATAAAATCCACAAATGTTGGTGG

mRNA sequence

TTCGAGAACGATGAATGTGGAACTGCAGAGGCCGCCTCTGAGAGAGATCAATCTTTAAGCTGAAGAGCCAAAGAAGAACACTCTTGAGGCATCGTTGAGGTAGAGAGTAGAGGAAGATGGAGAAAAAAGTTGGGGCTGAGATCGAAGAGCTTCCGAAGACTATAGTTCGCCGAGTGGTTAAAGAGAAGCTTTCTCAGTGCTCTCGGAACCAGGATATTTCCGTCAACAAAGATTCGCTTCTAGCCTTTTGCGAGAGCGCTCGGATCTTCATTCATTACCTCTCCGCAACGTCGAATGATATATGCAAGGAATCGAAGAGGCAGACGATTAAGGCGGAAGATGTATTGAAAGCTCTAGAAGATATGGAATTTCCCGAGTTGGTTAGGCCTCTTAAGGCTTCCCTCAATGAATTCAGAAGTAAGAATGCTGGAAAGAAGGCAGCTGCGTCGAGGATAAAAGAAGCGAAAAAGAGGAAGATAGAAGAAGAGCCTACAGTTGAAGGTACTGATGGTGAAAGCAAAGATGATGTCGAAAATGACAATGACGATAACGATAAAGATGAAGATGAAGTTGAAGATGAAGATGAAGTTGAGAATGGTGGCAGTGATGACTGAGAAACATGATGTATTTCCCTAATTAGGTACTTAAAATGAGGTTATTCCTCACTCTGTACGCATAAGATGGTTTACAAGTCTTCTAGGAATATATTTTTCTTTTTTCTGAGACTAATTTAGGTTGTATATTGTGAAGCGGCAGATGCACTTTTTGGCAAGTTCTGTATCTCTGTCCACATGGTAGTGAGAGAGGCTCCCATACCTTATTAAATCCATTTGTTTTCTGGGTTTTTCCCCATAGTTTTTCGAATGCCAGACATGAAGGACATGTTTGTTTCTTAGTTTCTAACGTATAAAATCCACAAATGTTGGTGG

Coding sequence (CDS)

ATGGAGAAAAAAGTTGGGGCTGAGATCGAAGAGCTTCCGAAGACTATAGTTCGCCGAGTGGTTAAAGAGAAGCTTTCTCAGTGCTCTCGGAACCAGGATATTTCCGTCAACAAAGATTCGCTTCTAGCCTTTTGCGAGAGCGCTCGGATCTTCATTCATTACCTCTCCGCAACGTCGAATGATATATGCAAGGAATCGAAGAGGCAGACGATTAAGGCGGAAGATGTATTGAAAGCTCTAGAAGATATGGAATTTCCCGAGTTGGTTAGGCCTCTTAAGGCTTCCCTCAATGAATTCAGAAGTAAGAATGCTGGAAAGAAGGCAGCTGCGTCGAGGATAAAAGAAGCGAAAAAGAGGAAGATAGAAGAAGAGCCTACAGTTGAAGGTACTGATGGTGAAAGCAAAGATGATGTCGAAAATGACAATGACGATAACGATAAAGATGAAGATGAAGTTGAAGATGAAGATGAAGTTGAGAATGGTGGCAGTGATGACTGA

Protein sequence

MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRKIEEEPTVEGTDGESKDDVENDNDDNDKDEDEVEDEDEVENGGSDD

Homology

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BLAST of Carg02642 vs. NCBI nr
Match: KAG7035929.1 (DNA polymerase epsilon subunit 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 292.7 bits (748), Expect = 1.9e-75
Identity = 165/165 (100.00%), Postives = 165/165 (100.00%), Query Frame = 0


Query: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60
           MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN
Sbjct: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120
           DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120

Query: 121 IEEEPTVEGTDGESKDDVENDNDDNDKDEDEVEDEDEVENGGSDD 166
           IEEEPTVEGTDGESKDDVENDNDDNDKDEDEVEDEDEVENGGSDD
Sbjct: 121 IEEEPTVEGTDGESKDDVENDNDDNDKDEDEVEDEDEVENGGSDD 165

[Close]

BLAST of Carg02642 vs. NCBI nr
Match: KAG6605978.1 (DNA polymerase epsilon subunit 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 283.9 bits (725), Expect = 9.0e-73
Identity = 164/173 (94.80%), Postives = 165/173 (95.38%), Query Frame = 0


Query: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60
           MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN
Sbjct: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120
           DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120

Query: 121 IEEEPTVEGTDGESKDDVENDNDDN--------DKDEDEVEDEDEVENGGSDD 166
           IEEEPTVEGTDGESKDDVENDNDDN        D+DEDEVEDEDEVENGGSDD
Sbjct: 121 IEEEPTVEGTDGESKDDVENDNDDNDDGNDKDKDEDEDEVEDEDEVENGGSDD 173

[Close]

BLAST of Carg02642 vs. NCBI nr
Match: XP_022995161.1 (DNA polymerase epsilon subunit 3 [Cucurbita maxima])

HSP 1 Score: 282.3 bits (721), Expect = 2.6e-72
Identity = 162/169 (95.86%), Postives = 164/169 (97.04%), Query Frame = 0


Query: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60
           MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESAR+FIHYLSATSN
Sbjct: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARVFIHYLSATSN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120
           DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120

Query: 121 IEEEPTVEGTDGESKDDVENDN----DDNDKDEDEVEDEDEVENGGSDD 166
           IEEEPTVEGTDGESKDDVENDN    DDNDKDEDE ED+DEVENGGSDD
Sbjct: 121 IEEEPTVEGTDGESKDDVENDNDDDDDDNDKDEDEDEDKDEVENGGSDD 169

[Close]

BLAST of Carg02642 vs. NCBI nr
Match: XP_023533668.1 (DNA polymerase epsilon subunit 3 [Cucurbita pepo subsp. pepo] >XP_023533669.1 DNA polymerase epsilon subunit 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 281.6 bits (719), Expect = 4.5e-72
Identity = 160/165 (96.97%), Postives = 161/165 (97.58%), Query Frame = 0


Query: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60
           MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN
Sbjct: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120
           DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120

Query: 121 IEEEPTVEGTDGESKDDVENDNDDNDKDEDEVEDEDEVENGGSDD 166
           IEEEPTVEGTDGESKDDVENDNDD+D   DE EDEDEVENGGSDD
Sbjct: 121 IEEEPTVEGTDGESKDDVENDNDDDDNGNDEDEDEDEVENGGSDD 165

[Close]

BLAST of Carg02642 vs. NCBI nr
Match: XP_022958709.1 (DNA polymerase epsilon subunit 3 [Cucurbita moschata] >XP_022958710.1 DNA polymerase epsilon subunit 3 [Cucurbita moschata])

HSP 1 Score: 281.6 bits (719), Expect = 4.5e-72
Identity = 160/165 (96.97%), Postives = 161/165 (97.58%), Query Frame = 0


Query: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60
           MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN
Sbjct: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120
           DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120

Query: 121 IEEEPTVEGTDGESKDDVENDNDDNDKDEDEVEDEDEVENGGSDD 166
           IEEEPTVEGTDGESKDDVENDNDDND   D+ EDEDEVENGGSDD
Sbjct: 121 IEEEPTVEGTDGESKDDVENDNDDNDDGNDKDEDEDEVENGGSDD 165

[Close]

BLAST of Carg02642 vs. ExPASy Swiss-Prot
Match: Q3SZN5 (DNA polymerase epsilon subunit 3 OS=Bos taurus OX=9913 GN=POLE3 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.6e-11
Identity = 48/149 (32.21%), Postives = 87/149 (58.39%), Query Frame = 0


Query: 12  LPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKESKRQTI 71
           LP  ++ R++KE L        ++++K++  A   +A +F+ Y ++ +N+   + KR+T+
Sbjct: 10  LPNAVITRIIKEALP-----DGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 72  KAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRKIEEEPTVEGTD 131
            A DVL A+E+MEF   V PLK +L  +R +  GKK A+ + K+ K +K + E      +
Sbjct: 70  NASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKKTDSE------E 129

Query: 132 GESKDDVENDNDDNDKDEDEVEDEDEVEN 161
            +   D +ND D+   +E+E  +E+EV+N
Sbjct: 130 QDKSRDEDNDEDEERLEEEEQNEEEEVDN 147

[Close]

BLAST of Carg02642 vs. ExPASy Swiss-Prot
Match: Q9NRF9 (DNA polymerase epsilon subunit 3 OS=Homo sapiens OX=9606 GN=POLE3 PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.6e-11
Identity = 48/149 (32.21%), Postives = 87/149 (58.39%), Query Frame = 0


Query: 12  LPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKESKRQTI 71
           LP  ++ R++KE L        ++++K++  A   +A +F+ Y ++ +N+   + KR+T+
Sbjct: 10  LPNAVITRIIKEALP-----DGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 72  KAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRKIEEEPTVEGTD 131
            A DVL A+E+MEF   V PLK +L  +R +  GKK A+ + K+ K +K + E      +
Sbjct: 70  NASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKKTDSE------E 129

Query: 132 GESKDDVENDNDDNDKDEDEVEDEDEVEN 161
            +   D +ND D+   +E+E  +E+EV+N
Sbjct: 130 QDKSRDEDNDEDEERLEEEEQNEEEEVDN 147

[Close]

BLAST of Carg02642 vs. ExPASy Swiss-Prot
Match: Q5R4W3 (DNA polymerase epsilon subunit 3 OS=Pongo abelii OX=9601 GN=POLE3 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.6e-11
Identity = 48/149 (32.21%), Postives = 87/149 (58.39%), Query Frame = 0


Query: 12  LPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKESKRQTI 71
           LP  ++ R++KE L        ++++K++  A   +A +F+ Y ++ +N+   + KR+T+
Sbjct: 10  LPNAVITRIIKEALP-----DGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 72  KAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRKIEEEPTVEGTD 131
            A DVL A+E+MEF   V PLK +L  +R +  GKK A+ + K+ K +K + E      +
Sbjct: 70  NASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKKTDSE------E 129

Query: 132 GESKDDVENDNDDNDKDEDEVEDEDEVEN 161
            +   D +ND D+   +E+E  +E+EV+N
Sbjct: 130 QDKSRDEDNDEDEERLEEEEQNEEEEVDN 147

[Close]

BLAST of Carg02642 vs. ExPASy Swiss-Prot
Match: Q642A5 (DNA polymerase epsilon subunit 3 OS=Rattus norvegicus OX=10116 GN=Pole3 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.0e-10
Identity = 50/149 (33.56%), Postives = 85/149 (57.05%), Query Frame = 0


Query: 12  LPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKESKRQTI 71
           LP  ++ R++KE L        ++++K++  A   +A +F+ Y ++ +N+   + KR+T+
Sbjct: 10  LPNAVITRIIKEALP-----DGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 72  KAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRKIEEEPTVEGTD 131
            A DVL A+E+MEF   V PLK +L  +R +  GKK A+ + K+ K +K  EE       
Sbjct: 70  NASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKKDCEE------- 129

Query: 132 GESKDDVENDNDDNDKDEDEVEDEDEVEN 161
            + K   E D D+   DE+E  +E+EV+N
Sbjct: 130 -QDKSREEEDEDEERLDEEEQNEEEEVDN 145

[Close]

BLAST of Carg02642 vs. ExPASy Swiss-Prot
Match: Q9JKP7 (DNA polymerase epsilon subunit 3 OS=Mus musculus OX=10090 GN=Pole3 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.5e-09
Identity = 46/149 (30.87%), Postives = 83/149 (55.70%), Query Frame = 0


Query: 12  LPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKESKRQTI 71
           LP  ++ R++KE L        ++++K++  A   +A +F+ Y ++ +N+   + KR+T+
Sbjct: 10  LPNAVITRIIKEALP-----DGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 72  KAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRKIEEEPTVEGTD 131
            A DVL A+E+MEF   + PLK +L  +R    GKK A+ + K+ K +K  EE       
Sbjct: 70  NASDVLSAMEEMEFQRFITPLKEALEAYRRDEKGKKEASEQKKKDKDKKDSEE------- 129

Query: 132 GESKDDVENDNDDNDKDEDEVEDEDEVEN 161
            + K     + D+   DED+  +E+E++N
Sbjct: 130 -QDKSREVEEEDEERLDEDDQNEEEEIDN 145

[Close]

BLAST of Carg02642 vs. ExPASy TrEMBL
Match: A0A6J1K184 (DNA polymerase epsilon subunit 3 OS=Cucurbita maxima OX=3661 GN=LOC111490783 PE=4 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 1.3e-72
Identity = 162/169 (95.86%), Postives = 164/169 (97.04%), Query Frame = 0


Query: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60
           MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESAR+FIHYLSATSN
Sbjct: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARVFIHYLSATSN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120
           DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120

Query: 121 IEEEPTVEGTDGESKDDVENDN----DDNDKDEDEVEDEDEVENGGSDD 166
           IEEEPTVEGTDGESKDDVENDN    DDNDKDEDE ED+DEVENGGSDD
Sbjct: 121 IEEEPTVEGTDGESKDDVENDNDDDDDDNDKDEDEDEDKDEVENGGSDD 169

[Close]

BLAST of Carg02642 vs. ExPASy TrEMBL
Match: A0A6J1H495 (DNA polymerase epsilon subunit 3 OS=Cucurbita moschata OX=3662 GN=LOC111459853 PE=4 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 2.2e-72
Identity = 160/165 (96.97%), Postives = 161/165 (97.58%), Query Frame = 0


Query: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60
           MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN
Sbjct: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120
           DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120

Query: 121 IEEEPTVEGTDGESKDDVENDNDDNDKDEDEVEDEDEVENGGSDD 166
           IEEEPTVEGTDGESKDDVENDNDDND   D+ EDEDEVENGGSDD
Sbjct: 121 IEEEPTVEGTDGESKDDVENDNDDNDDGNDKDEDEDEVENGGSDD 165

[Close]

BLAST of Carg02642 vs. ExPASy TrEMBL
Match: A0A6J1DJA4 (DNA polymerase epsilon subunit 3 OS=Momordica charantia OX=3673 GN=LOC111020575 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 2.5e-60
Identity = 145/167 (86.83%), Postives = 153/167 (91.62%), Query Frame = 0


Query: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60
           MEKKV AEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN
Sbjct: 1   MEKKVAAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120
           DICKESKRQTIKA+DVLKALEDMEFPELVRPLKASLNEFR K+AGKKAAASR KEAKK++
Sbjct: 61  DICKESKRQTIKADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKR 120

Query: 121 IEEEPTVEGTDGESKDDVENDNDDN--DKDEDEVEDEDEVENGGSDD 166
             EEPTVE    ESKDDV ND++DN  D+DEDEVEDEDE ENG SD+
Sbjct: 121 KIEEPTVE---DESKDDVGNDDNDNDHDEDEDEVEDEDEEENGASDE 164

[Close]

BLAST of Carg02642 vs. ExPASy TrEMBL
Match: A0A0A0KEV7 (CBFD_NFYB_HMF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G425700 PE=4 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 3.0e-58
Identity = 141/166 (84.94%), Postives = 147/166 (88.55%), Query Frame = 0


Query: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60
           MEKK+G   EELPKTIVRRVVKEKLSQCSR+QDISVNKDSLLAFCESARIFIHYLSAT+N
Sbjct: 1   MEKKIGGGNEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120
           DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASL+EFR KNAGKKAAASR KEAKK++
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKR 120

Query: 121 IEEEPTVEGTDGESKDDVEN-DNDDNDKDEDEVEDEDEVENGGSDD 166
             EEPTVEGTDGES DDV N DNDDND      EDEDE EN  SDD
Sbjct: 121 KVEEPTVEGTDGESIDDVGNDDNDDND------EDEDEDENRASDD 160

[Close]

BLAST of Carg02642 vs. ExPASy TrEMBL
Match: A0A1S3BNA4 (DNA polymerase epsilon subunit D OS=Cucumis melo OX=3656 GN=LOC103491421 PE=4 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 3.7e-56
Identity = 130/154 (84.42%), Postives = 140/154 (90.91%), Query Frame = 0


Query: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60
           MEKK+ A IEELPKTIVRRVVKEKLSQCSR+QDISVNKDSLLAFCESARIFIHYLSAT+N
Sbjct: 1   MEKKIVAGIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120
           DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFR KNAGKKAAA R KE KK++
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRRKNAGKKAAALREKEVKKKR 120

Query: 121 IEEEPTVEGTDGESKDDVENDNDDNDKDEDEVED 155
             EEPTVEGTDGES D V +D++D D+DE+E  D
Sbjct: 121 KVEEPTVEGTDGESIDGVGDDDNDEDEDENEASD 154

[Close]

BLAST of Carg02642 vs. TAIR 10
Match: AT2G27470.1 (nuclear factor Y, subunit B11 )

HSP 1 Score: 138.3 bits (347), Expect = 5.7e-33
Identity = 93/194 (47.94%), Postives = 127/194 (65.46%), Query Frame = 0


Query: 9   IEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKESKR 68
           ++ELP  IVRRVVK+KLS+CS + D+S++K++LLAF ESARIFIHYLSAT+ND CK+++R
Sbjct: 8   VDELPLAIVRRVVKKKLSECSPDYDVSIHKEALLAFSESARIFIHYLSATANDFCKDARR 67

Query: 69  QTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKA-------------------A 128
           QT+KA+DV KALE+M+F E + PLK+SL +F+ KNAGKKA                    
Sbjct: 68  QTMKADDVFKALEEMDFSEFLEPLKSSLEDFKKKNAGKKAGAAAASYPAGGAALKSSSGT 127

Query: 129 ASRIKEAKKRKIEEEPTVEGT-----DGESK---DDVENDN----------DDNDKDEDE 166
           AS+ KE KKRK EE  T +G      D E+K   ++ ENDN          D+N  DE++
Sbjct: 128 ASKPKETKKRKQEEPSTQKGARKSKIDEETKRNDEETENDNTEEENGNDEEDENGNDEED 187

[Close]

BLAST of Carg02642 vs. TAIR 10
Match: AT5G23090.2 (nuclear factor Y, subunit B13 )

HSP 1 Score: 48.1 bits (113), Expect = 7.7e-06
Identity = 29/93 (31.18%), Postives = 50/93 (53.76%), Query Frame = 0


Query: 8   EIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKESK 67
           E   LPK  + +++KE L       D+ V +D+     E    FI+ +S+ SND+C +  
Sbjct: 12  EDASLPKATMTKIIKEMLP-----PDVRVARDAQDLLIECCVEFINLVSSESNDVCNKED 71

Query: 68  RQTIKAEDVLKALEDMEFPELVRPLKASLNEFR 101
           ++TI  E VLKAL+ + F E +  + A+  + +
Sbjct: 72  KRTIAPEHVLKALQVLGFGEYIEEVYAAYEQHK 99

[Close]

BLAST of Carg02642 vs. TAIR 10
Match: AT5G23090.1 (nuclear factor Y, subunit B13 )

HSP 1 Score: 48.1 bits (113), Expect = 7.7e-06
Identity = 29/93 (31.18%), Postives = 50/93 (53.76%), Query Frame = 0


Query: 8   EIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKESK 67
           E   LPK  + +++KE L       D+ V +D+     E    FI+ +S+ SND+C +  
Sbjct: 12  EDASLPKATMTKIIKEMLP-----PDVRVARDAQDLLIECCVEFINLVSSESNDVCNKED 71

Query: 68  RQTIKAEDVLKALEDMEFPELVRPLKASLNEFR 101
           ++TI  E VLKAL+ + F E +  + A+  + +
Sbjct: 72  KRTIAPEHVLKALQVLGFGEYIEEVYAAYEQHK 99

[Close]

BLAST of Carg02642 vs. TAIR 10
Match: AT5G23090.4 (nuclear factor Y, subunit B13 )

HSP 1 Score: 48.1 bits (113), Expect = 7.7e-06
Identity = 29/93 (31.18%), Postives = 50/93 (53.76%), Query Frame = 0


Query: 8   EIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKESK 67
           E   LPK  + +++KE L       D+ V +D+     E    FI+ +S+ SND+C +  
Sbjct: 12  EDASLPKATMTKIIKEMLP-----PDVRVARDAQDLLIECCVEFINLVSSESNDVCNKED 71

Query: 68  RQTIKAEDVLKALEDMEFPELVRPLKASLNEFR 101
           ++TI  E VLKAL+ + F E +  + A+  + +
Sbjct: 72  KRTIAPEHVLKALQVLGFGEYIEEVYAAYEQHK 99

[Close]

BLAST of Carg02642 vs. TAIR 10
Match: AT5G08190.1 (nuclear factor Y, subunit B12 )

HSP 1 Score: 47.4 bits (111), Expect = 1.3e-05
Identity = 29/93 (31.18%), Postives = 50/93 (53.76%), Query Frame = 0


Query: 8   EIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSNDICKESK 67
           E   LPK  + +++KE L       D+ V +D+     E    FI+ +S+ SN++C +  
Sbjct: 12  EDASLPKATMTKIIKEMLP-----ADVRVARDAQDLLIECCVEFINLISSESNEVCNKED 71

Query: 68  RQTIKAEDVLKALEDMEFPELVRPLKASLNEFR 101
           ++TI  E VLKAL+ + F E V  + A+  + +
Sbjct: 72  KRTIAPEHVLKALQVLGFGEYVEEVYAAYEQHK 99

The following BLAST results are available for this feature:

BLAST of Carg02642 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7035929.1	1.9e-75	100.00	DNA polymerase epsilon subunit 3 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6605978.1	9.0e-73	94.80	DNA polymerase epsilon subunit 3, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022995161.1	2.6e-72	95.86	DNA polymerase epsilon subunit 3 [Cucurbita maxima]	[more]
XP_023533668.1	4.5e-72	96.97	DNA polymerase epsilon subunit 3 [Cucurbita pepo subsp. pepo] >XP_023533669.1 DN...	[more]
XP_022958709.1	4.5e-72	96.97	DNA polymerase epsilon subunit 3 [Cucurbita moschata] >XP_022958710.1 DNA polyme...	[more]

BLAST of Carg02642 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3SZN5	4.6e-11	32.21	DNA polymerase epsilon subunit 3 OS=Bos taurus OX=9913 GN=POLE3 PE=2 SV=1	[more]
Q9NRF9	4.6e-11	32.21	DNA polymerase epsilon subunit 3 OS=Homo sapiens OX=9606 GN=POLE3 PE=1 SV=1	[more]
Q5R4W3	4.6e-11	32.21	DNA polymerase epsilon subunit 3 OS=Pongo abelii OX=9601 GN=POLE3 PE=2 SV=1	[more]
Q642A5	1.0e-10	33.56	DNA polymerase epsilon subunit 3 OS=Rattus norvegicus OX=10116 GN=Pole3 PE=1 SV=...	[more]
Q9JKP7	1.5e-09	30.87	DNA polymerase epsilon subunit 3 OS=Mus musculus OX=10090 GN=Pole3 PE=1 SV=1	[more]

BLAST of Carg02642 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K184	1.3e-72	95.86	DNA polymerase epsilon subunit 3 OS=Cucurbita maxima OX=3661 GN=LOC111490783 PE=...	[more]
A0A6J1H495	2.2e-72	96.97	DNA polymerase epsilon subunit 3 OS=Cucurbita moschata OX=3662 GN=LOC111459853 P...	[more]
A0A6J1DJA4	2.5e-60	86.83	DNA polymerase epsilon subunit 3 OS=Momordica charantia OX=3673 GN=LOC111020575 ...	[more]
A0A0A0KEV7	3.0e-58	84.94	CBFD_NFYB_HMF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G4257...	[more]
A0A1S3BNA4	3.7e-56	84.42	DNA polymerase epsilon subunit D OS=Cucumis melo OX=3656 GN=LOC103491421 PE=4 SV...	[more]

BLAST of Carg02642 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G27470.1	5.7e-33	47.94	nuclear factor Y, subunit B11	[more]
AT5G23090.2	7.7e-06	31.18	nuclear factor Y, subunit B13	[more]
AT5G23090.1	7.7e-06	31.18	nuclear factor Y, subunit B13	[more]
AT5G23090.4	7.7e-06	31.18	nuclear factor Y, subunit B13	[more]
AT5G08190.1	1.3e-05	31.18	nuclear factor Y, subunit B12	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 135..155
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 100..165
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 108..133
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 134..165
None	No IPR available	PANTHER	PTHR46172	DNA POLYMERASE EPSILON SUBUNIT 3	coord: 3..158
IPR009072	Histone-fold	GENE3D	1.10.20.10	Histone, subunit A	coord: 3..160 e-value: 8.6E-33 score: 115.0
IPR009072	Histone-fold	SUPERFAMILY	47113	Histone-fold	coord: 12..136
IPR003958	Transcription factor CBF/NF-Y/archaeal histone domain	PFAM	PF00808	CBFD_NFYB_HMF	coord: 11..80 e-value: 7.2E-9 score: 35.8

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg02642-RA	Carg02642-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006974	cellular response to DNA damage stimulus
biological_process	GO:0031507	heterochromatin assembly
biological_process	GO:0006272	leading strand elongation
biological_process	GO:0042766	nucleosome mobilization
cellular_component	GO:0008623	CHRAC
cellular_component	GO:0008622	epsilon DNA polymerase complex
molecular_function	GO:0031490	chromatin DNA binding
molecular_function	GO:0046982	protein heterodimerization activity