Carg02563 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg02563
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionaluminum-activated malate transporter 9-like
LocationCarg_Chr02: 8072173 .. 8075452 (-)
RNA-Seq ExpressionCarg02563
SyntenyCarg02563
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATGGAAAAACAGGCAATACCGTTATAGACATCGATTTCTCGACAAAACCCGAACCTATAGGGACGAAAGCTGAAAACTTGGCAGCTTCATGTAAAGCTTCAATAGGGAGTGTTTTGGGGTTTTGCAAAGAAGACAGCAACCGAGTTTTATTCTCATTGAAAGTTGGGTTGGCTGTTGTTTTAGTGTCTTTGCTTATACTTTTTCGAGCACCTTACGATGTGTTTGGGACTAATATCATTTGGGCTATCATCACTGTAGCCATCATGTTCGAATATACCGTTGGTTTGTAGCTATCCATAAACCCCACGTTTTGTCTTTTGATGATTTTAATGCATATTATGTTATAATGGTAATGAAGTTTTTTGGGGTTTTGTTGTCAAAAGGAGCAACTTTCAACCGTGGTTTCAATAGGGCGCTCGGAAGCTTGCTTGCTGGAATCTTGGCCATTGCTGTTGCCCAACTTGCGCTACGCACAGGCCCTATTGCAGAACCCATTATCATTGGCATTACCATTTTCCTAGTTGGTAACTTTCTCACCAAAGTTTACGTTTTAATGTTTAAATTATAAACGTAACGGTCTACCTAAGTCCACCGCTAGTAGATATATTGTTCTTTCTGGGTTTTCCTTTTCGGGCTTTCCCTCAAAGTTTTTAAAACGTGTCAGTTAAGCATAGGTTTCCACATGTTTACAAAGAGTGTTTCGTTCTCTTCCCCAATCGATGTGGGATCTCACAATCCACTCCCCTTCGGGACCCAGCATCCTCGCCGACATTCATTACTTTTTCCAACCGATGTAGATTCCATCCAAATCTACCCCCTCGGGGCTCAACGTCCTTACTGGCACATCGCCTCATGTCACCCCCTTCAGGGCTTAGCCTTCTCACTCGCACATCGTCTCATGTCCACCCCTTCAAAGCTTAGCCTCCTCGCTGGCACATCGCCGTGTGTCTGTGGGATCTCACAATCCACTCCCCTTTGAGGCCCAGTGTCCTCGCCGACACTCGTTACTTTCTCCAATAGGTGTGGGATCCATCCAAATCCACCTCCTTTAGGCCCAACGTCCTGACACATTGTCTCATGTCCACCTTCTTCAGGGCTTAGCCTCCTCACTAGCACATCGCCCGGTATCTGACTTTGATACCATTTGTACCAGCTCAAGTCCACCGCTAATAAATATTTTCCTTTTTAGACTTTTCCTTGGGCCTCCTTAAAATGCGTCCCTCTCTTGTAAATGGTGTTTTGTTTTACTTACGGAGGTTGGAGGTTAGTATATGAATGATAGTAATAGTAAATTATATAAGTAATGTGAAAGTGTGTTGAACAGGAAGTGTGACATCATTCATGAAGCTATGGCCATCGCTAGTTCCATACGAGTATGGGTTCAGAGTTATACTTTTCACATACTGCTTGATAGTTGTTTCTGGTTATCGAATGGGGAACCCACTGAGAACTGCCATGGATCGCCTCTACTCCATCGCCATCGGAGGATTCGTTGCAGTGTTAGTGAACGTCCTCGTTTTCCCCATCTGGGCTGGCGAGCAGCTGCACGAGGACCTCGTCGCCACCTTCAACTCCCTCGCTGATTCCCTCCAAGGTTCGAACTTTTGGTTCAACTTTTAACATTCTCTCATTTACTTCAAAAACAATGTTACAAATTTGGCAGAATGTGTGAACAAGTATTTAGAGGACGAGGGATCAAACATTGGGCAACAGCTTCCCAAGGCTGTAATGGATGAATTTCCAGACGAACCAGCTTATAAGAAGTGCAAAGCCACCTTGAACTCCTCTGCAAAGTTTGAGGCTTTGGTACGCTAAAATTTACCCTTTTTCTTCTTATGAACACTATGATTCTTAATTTCTAATCCAATGGAAATGACCATTTTTTAGGCGACATCCGCGAAATGGGAGCCGCCACACGGCAGATTCGGGCACTTCTTTTATCCATGGGGGGAGTATGTTAAAGTTGGAGCTGTTTTGAGATATTGTGCTTATGAAATTATGGCTCTTCATGGTGTTCTTCACTCTCCAATCCAGGTCATCCTCTTAGCTTCATCTCTTGTTCCAAGTCTTGTGGGTGTTTTTAGCATGAAATTTAGAAGGAAAAAATTGAAGGTTATCTGCTTTGACCCGTTACTGATCGTATCGCCGTCAGTATCACAGTTTTGAAACGTATCTGCTAAAGAGAGTTTCTACATCCTTATAAAGAATGTTTCGTTCCCTCTTCAACCGATGTGGAATATCACAATCCACCCTCCTTGGGGGCCAGTGTCCTCGCTGACACATACCATTTGTAACCGCCAAAGTCCACTGCTAACGGATATTCAACCGATGTGGAATCTCACAATCCACCCTCCTTGGGGGCCAGTGTCCTCGTTGGCACATACCATTTGTAACCGCCAAAGTCCACTACTAACGGATATTCAACCGATGTGGGATCTCACAATCCACCCTCCTTGGGGGCCAGTGTCCTCGATGGCACATACCATTTGTAACCGTCAAAGCCCACCGCTAACGGATATTGTCCATTTTGGCCTATTACGTATCGCCGTTAACCTCACAGTTTTAAAATGTGTCTGATCTTCTTAGACAAAATTCAATTTTATATTCGATAGATTCATTTTATATCTCTATCTTACTGAACTTTTGTAGGCTCCATATAAGCTCAGAATCACATTCAAATCCGAGATCCAAGAAACAGCAAACCAAGCAGCGGAGCTGTTGAGAAGTTTGGGTAAAGACATCAACAACATGCAACAATCCCTCAAAATATATCTCCTAAAGAATGTTCATACTGCAGCAGACAAGCTGCAACAAGCCCTTCACACTCACTCTTATCCCCTCACTCCCACTTGTGACTCCCTAGACCTCTCTTCCAAGCTTCAGTCCAAGCTCTTGACTGCCTCGTCGAACCGCCTCTATGATCTTCCAAGCTTGTTAGCTGAGCTCAACGGCAATGGCCCGGAGAGGAGTTTAATCCAGCAGAATAGTCATGGTGTGGCTGCAGGGACTCAAGCAGAGGCTTACGTTGAGTTAGTGAGGAAGCAGTCGAGAAGGCAGCATTCATGGCCGTTGAGGGAAATGGATGTTTTCGACGACGGTGGGGGTGTTCCCATCGAGTATTTACAGAGGATGAGGAAGCAAGAGAGTACTGCAGCCATGTCACTGGCTAACTTTACTTCCTTGCTCATCGAGTTCGTGGCTAGGCTCGACTATTTGGTCGAAACCGTGGATGAGCTTTCTAAGATGGCCAAGTTCAAAGATAATATCATTAGCTGA

mRNA sequence

ATGAATGGAAAAACAGGCAATACCGTTATAGACATCGATTTCTCGACAAAACCCGAACCTATAGGGACGAAAGCTGAAAACTTGGCAGCTTCATGTAAAGCTTCAATAGGGAGTGTTTTGGGGTTTTGCAAAGAAGACAGCAACCGAGTTTTATTCTCATTGAAAGTTGGGTTGGCTGTTGTTTTAGTGTCTTTGCTTATACTTTTTCGAGCACCTTACGATGTGTTTGGGACTAATATCATTTGGGCTATCATCACTGTAGCCATCATGTTCGAATATACCGTTGGAGCAACTTTCAACCGTGGTTTCAATAGGGCGCTCGGAAGCTTGCTTGCTGGAATCTTGGCCATTGCTCTATGGCCATCGCTAGTTCCATACGAGTATGGGTTCAGAGTTATACTTTTCACATACTGCTTGATAGTTGTTTCTGGTTATCGAATGGGGAACCCACTGAGAACTGCCATGGATCGCCTCTACTCCATCGCCATCGGAGGATTCGTTGCAGTGTTAGTGAACGTCCTCGTTTTCCCCATCTGGGCTGGCGAGCAGCTGCACGAGGACCTCGTCGCCACCTTCAACTCCCTCGCTGATTCCCTCCAAGAATGTGTGAACAAGTATTTAGAGGACGAGGGATCAAACATTGGGCAACAGCTTCCCAAGGCTGTAATGGATGAATTTCCAGACGAACCAGCTTATAAGAAGTGCAAAGCCACCTTGAACTCCTCTGCAAAGTTTGAGGCTTTGGCGACATCCGCGAAATGGGAGCCGCCACACGGCAGATTCGGGCACTTCTTTTATCCATGGGGGGAGTATGTTAAAGTTGGAGCTGTTTTGAGATATTGTGCTTATGAAATTATGGCTCTTCATGGTGTTCTTCACTCTCCAATCCAGATCCAAGAAACAGCAAACCAAGCAGCGGAGCTGTTGAGAAGTTTGGGTAAAGACATCAACAACATGCAACAATCCCTCAAAATATATCTCCTAAAGAATGTTCATACTGCAGCAGACAAGCTGCAACAAGCCCTTCACACTCACTCTTATCCCCTCACTCCCACTTGTGACTCCCTAGACCTCTCTTCCAAGCTTCAGTCCAAGCTCTTGACTGCCTCGTCGAACCGCCTCTATGATCTTCCAAGCTTGTTAGCTGAGCTCAACGGCAATGGCCCGGAGAGGAGTTTAATCCAGCAGAATAGTCATGGTGTGGCTGCAGGGACTCAAGCAGAGGCTTACGTTGAGTTAGTGAGGAAGCAGTCGAGAAGGCAGCATTCATGGCCGTTGAGGGAAATGGATGTTTTCGACGACGGTGGGGGTGTTCCCATCGAGTATTTACAGAGGATGAGGAAGCAAGAGAGTACTGCAGCCATGTCACTGGCTAACTTTACTTCCTTGCTCATCGAGTTCGTGGCTAGGCTCGACTATTTGGTCGAAACCGTGGATGAGCTTTCTAAGATGGCCAAGTTCAAAGATAATATCATTAGCTGA

Coding sequence (CDS)

ATGAATGGAAAAACAGGCAATACCGTTATAGACATCGATTTCTCGACAAAACCCGAACCTATAGGGACGAAAGCTGAAAACTTGGCAGCTTCATGTAAAGCTTCAATAGGGAGTGTTTTGGGGTTTTGCAAAGAAGACAGCAACCGAGTTTTATTCTCATTGAAAGTTGGGTTGGCTGTTGTTTTAGTGTCTTTGCTTATACTTTTTCGAGCACCTTACGATGTGTTTGGGACTAATATCATTTGGGCTATCATCACTGTAGCCATCATGTTCGAATATACCGTTGGAGCAACTTTCAACCGTGGTTTCAATAGGGCGCTCGGAAGCTTGCTTGCTGGAATCTTGGCCATTGCTCTATGGCCATCGCTAGTTCCATACGAGTATGGGTTCAGAGTTATACTTTTCACATACTGCTTGATAGTTGTTTCTGGTTATCGAATGGGGAACCCACTGAGAACTGCCATGGATCGCCTCTACTCCATCGCCATCGGAGGATTCGTTGCAGTGTTAGTGAACGTCCTCGTTTTCCCCATCTGGGCTGGCGAGCAGCTGCACGAGGACCTCGTCGCCACCTTCAACTCCCTCGCTGATTCCCTCCAAGAATGTGTGAACAAGTATTTAGAGGACGAGGGATCAAACATTGGGCAACAGCTTCCCAAGGCTGTAATGGATGAATTTCCAGACGAACCAGCTTATAAGAAGTGCAAAGCCACCTTGAACTCCTCTGCAAAGTTTGAGGCTTTGGCGACATCCGCGAAATGGGAGCCGCCACACGGCAGATTCGGGCACTTCTTTTATCCATGGGGGGAGTATGTTAAAGTTGGAGCTGTTTTGAGATATTGTGCTTATGAAATTATGGCTCTTCATGGTGTTCTTCACTCTCCAATCCAGATCCAAGAAACAGCAAACCAAGCAGCGGAGCTGTTGAGAAGTTTGGGTAAAGACATCAACAACATGCAACAATCCCTCAAAATATATCTCCTAAAGAATGTTCATACTGCAGCAGACAAGCTGCAACAAGCCCTTCACACTCACTCTTATCCCCTCACTCCCACTTGTGACTCCCTAGACCTCTCTTCCAAGCTTCAGTCCAAGCTCTTGACTGCCTCGTCGAACCGCCTCTATGATCTTCCAAGCTTGTTAGCTGAGCTCAACGGCAATGGCCCGGAGAGGAGTTTAATCCAGCAGAATAGTCATGGTGTGGCTGCAGGGACTCAAGCAGAGGCTTACGTTGAGTTAGTGAGGAAGCAGTCGAGAAGGCAGCATTCATGGCCGTTGAGGGAAATGGATGTTTTCGACGACGGTGGGGGTGTTCCCATCGAGTATTTACAGAGGATGAGGAAGCAAGAGAGTACTGCAGCCATGTCACTGGCTAACTTTACTTCCTTGCTCATCGAGTTCGTGGCTAGGCTCGACTATTTGGTCGAAACCGTGGATGAGCTTTCTAAGATGGCCAAGTTCAAAGATAATATCATTAGCTGA

Protein sequence

MNGKTGNTVIDIDFSTKPEPIGTKAENLAASCKASIGSVLGFCKEDSNRVLFSLKVGLAVVLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIALWPSLVPYEYGFRVILFTYCLIVVSGYRMGNPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHEDLVATFNSLADSLQECVNKYLEDEGSNIGQQLPKAVMDEFPDEPAYKKCKATLNSSAKFEALATSAKWEPPHGRFGHFFYPWGEYVKVGAVLRYCAYEIMALHGVLHSPIQIQETANQAAELLRSLGKDINNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTCDSLDLSSKLQSKLLTASSNRLYDLPSLLAELNGNGPERSLIQQNSHGVAAGTQAEAYVELVRKQSRRQHSWPLREMDVFDDGGGVPIEYLQRMRKQESTAAMSLANFTSLLIEFVARLDYLVETVDELSKMAKFKDNIIS
Homology
BLAST of Carg02563 vs. NCBI nr
Match: KAG7036007.1 (putative aluminum-activated malate transporter 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 967.6 bits (2500), Expect = 4.0e-278
Identity = 493/493 (100.00%), Postives = 493/493 (100.00%), Query Frame = 0

Query: 1   MNGKTGNTVIDIDFSTKPEPIGTKAENLAASCKASIGSVLGFCKEDSNRVLFSLKVGLAV 60
           MNGKTGNTVIDIDFSTKPEPIGTKAENLAASCKASIGSVLGFCKEDSNRVLFSLKVGLAV
Sbjct: 1   MNGKTGNTVIDIDFSTKPEPIGTKAENLAASCKASIGSVLGFCKEDSNRVLFSLKVGLAV 60

Query: 61  VLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIALW 120
           VLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIALW
Sbjct: 61  VLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIALW 120

Query: 121 PSLVPYEYGFRVILFTYCLIVVSGYRMGNPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWA 180
           PSLVPYEYGFRVILFTYCLIVVSGYRMGNPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWA
Sbjct: 121 PSLVPYEYGFRVILFTYCLIVVSGYRMGNPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWA 180

Query: 181 GEQLHEDLVATFNSLADSLQECVNKYLEDEGSNIGQQLPKAVMDEFPDEPAYKKCKATLN 240
           GEQLHEDLVATFNSLADSLQECVNKYLEDEGSNIGQQLPKAVMDEFPDEPAYKKCKATLN
Sbjct: 181 GEQLHEDLVATFNSLADSLQECVNKYLEDEGSNIGQQLPKAVMDEFPDEPAYKKCKATLN 240

Query: 241 SSAKFEALATSAKWEPPHGRFGHFFYPWGEYVKVGAVLRYCAYEIMALHGVLHSPIQIQE 300
           SSAKFEALATSAKWEPPHGRFGHFFYPWGEYVKVGAVLRYCAYEIMALHGVLHSPIQIQE
Sbjct: 241 SSAKFEALATSAKWEPPHGRFGHFFYPWGEYVKVGAVLRYCAYEIMALHGVLHSPIQIQE 300

Query: 301 TANQAAELLRSLGKDINNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTCDSLDLSS 360
           TANQAAELLRSLGKDINNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTCDSLDLSS
Sbjct: 301 TANQAAELLRSLGKDINNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTCDSLDLSS 360

Query: 361 KLQSKLLTASSNRLYDLPSLLAELNGNGPERSLIQQNSHGVAAGTQAEAYVELVRKQSRR 420
           KLQSKLLTASSNRLYDLPSLLAELNGNGPERSLIQQNSHGVAAGTQAEAYVELVRKQSRR
Sbjct: 361 KLQSKLLTASSNRLYDLPSLLAELNGNGPERSLIQQNSHGVAAGTQAEAYVELVRKQSRR 420

Query: 421 QHSWPLREMDVFDDGGGVPIEYLQRMRKQESTAAMSLANFTSLLIEFVARLDYLVETVDE 480
           QHSWPLREMDVFDDGGGVPIEYLQRMRKQESTAAMSLANFTSLLIEFVARLDYLVETVDE
Sbjct: 421 QHSWPLREMDVFDDGGGVPIEYLQRMRKQESTAAMSLANFTSLLIEFVARLDYLVETVDE 480

Query: 481 LSKMAKFKDNIIS 494
           LSKMAKFKDNIIS
Sbjct: 481 LSKMAKFKDNIIS 493

BLAST of Carg02563 vs. NCBI nr
Match: KAG6606059.1 (Aluminum-activated malate transporter 9, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 939.1 bits (2426), Expect = 1.5e-269
Identity = 491/537 (91.43%), Postives = 492/537 (91.62%), Query Frame = 0

Query: 1   MNGKTGNTVIDIDFSTKPEPIGTKAENLAASCKASIGSVLGFCKEDSNRVLFSLKVGLAV 60
           MNGKTGNTVIDIDFSTKPEPIGTKAENLAASCKASIGSVLGFCKEDSNRVLFSLKVGLAV
Sbjct: 1   MNGKTGNTVIDIDFSTKPEPIGTKAENLAASCKASIGSVLGFCKEDSNRVLFSLKVGLAV 60

Query: 61  VLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIA-- 120
           VLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIA  
Sbjct: 61  VLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIAVA 120

Query: 121 ------------------------------LWPSLVPYEYGFRVILFTYCLIVVSGYRMG 180
                                         LWPSLVPYEYGFRVILFTYCLIVVSGYRMG
Sbjct: 121 QLALRTGPIAEPIIIGITIFLVGSVTSFMKLWPSLVPYEYGFRVILFTYCLIVVSGYRMG 180

Query: 181 NPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHEDLVATFNSLADSLQECVNKYLE 240
           NPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHEDLVATFNSLADSLQECVNKYLE
Sbjct: 181 NPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHEDLVATFNSLADSLQECVNKYLE 240

Query: 241 DEGSNIGQQLPKAVMDEFPDEPAYKKCKATLNSSAKFEALATSAKWEPPHGRFGHFFYPW 300
           DEGSNIGQQLPKAVMDEFPDEPAYKKCKATLNSSAKFEALATSAKWEPPHGRFGHFFYPW
Sbjct: 241 DEGSNIGQQLPKAVMDEFPDEPAYKKCKATLNSSAKFEALATSAKWEPPHGRFGHFFYPW 300

Query: 301 GEYVKVGAVLRYCAYEIMALHGVLHSPIQ------------IQETANQAAELLRSLGKDI 360
           GEYVKVGAVLRYCAYEIMALHGVLHSPIQ            IQETANQAAELLRSLGKDI
Sbjct: 301 GEYVKVGAVLRYCAYEIMALHGVLHSPIQAPYKLRITFKSEIQETANQAAELLRSLGKDI 360

Query: 361 NNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTCDSLDLSSKLQSKLLTASSNRLYD 420
           NNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTCDSLDLSSKLQSKLLTASSNRLYD
Sbjct: 361 NNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTCDSLDLSSKLQSKLLTASSNRLYD 420

Query: 421 LPSLLAELNGNGPERSLIQQNSHGVAAGTQAEAYVELVRKQSRRQHSWPLREMDVFDDGG 480
           LPSLLAELNGNGPERSLIQQNSHGVAAGTQAEAYVELVRKQSRRQHSWPLREMDVFDDGG
Sbjct: 421 LPSLLAELNGNGPERSLIQQNSHGVAAGTQAEAYVELVRKQSRRQHSWPLREMDVFDDGG 480

Query: 481 GVPIEYLQRMRKQESTAAMSLANFTSLLIEFVARLDYLVETVDELSKMAKFKDNIIS 494
           GVP+EYLQRMRKQESTAAMSLANFTSLLIEFVARLDYLVETVD LSKMAKFKDNIIS
Sbjct: 481 GVPVEYLQRMRKQESTAAMSLANFTSLLIEFVARLDYLVETVDGLSKMAKFKDNIIS 537

BLAST of Carg02563 vs. NCBI nr
Match: XP_022957880.1 (aluminum-activated malate transporter 9-like [Cucurbita moschata])

HSP 1 Score: 925.2 bits (2390), Expect = 2.3e-265
Identity = 484/537 (90.13%), Postives = 487/537 (90.69%), Query Frame = 0

Query: 1   MNGKTGNTVIDIDFSTKPEPIGTKAENLAASCKASIGSVLGFCKEDSNRVLFSLKVGLAV 60
           MNGKTGNTVIDIDFSTKPEPIG KAENLAASCKASIGSVLGFCKEDSNRVLFSLKVGLAV
Sbjct: 1   MNGKTGNTVIDIDFSTKPEPIGAKAENLAASCKASIGSVLGFCKEDSNRVLFSLKVGLAV 60

Query: 61  VLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIA-- 120
           VLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIA  
Sbjct: 61  VLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIAVA 120

Query: 121 ------------------------------LWPSLVPYEYGFRVILFTYCLIVVSGYRMG 180
                                         LWPSLVPYEYGFRVILFTYCLIVVSGYRMG
Sbjct: 121 QLALRTGPIAEPIIIGISIFLVGSVTSFMKLWPSLVPYEYGFRVILFTYCLIVVSGYRMG 180

Query: 181 NPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHEDLVATFNSLADSLQECVNKYLE 240
           NPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLH+DLVATFNSLADSLQECVNKYLE
Sbjct: 181 NPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHKDLVATFNSLADSLQECVNKYLE 240

Query: 241 DEGSNIGQQLPKAVMDEFPDEPAYKKCKATLNSSAKFEALATSAKWEPPHGRFGHFFYPW 300
           DEGSNIGQQLPKAVMDEFPDEPAYKKCKATLNSSAKFEALATSAKWEPPHGRFGHFFYPW
Sbjct: 241 DEGSNIGQQLPKAVMDEFPDEPAYKKCKATLNSSAKFEALATSAKWEPPHGRFGHFFYPW 300

Query: 301 GEYVKVGAVLRYCAYEIMALHGVLHSPIQ------------IQETANQAAELLRSLGKDI 360
           GEYVKVGAVLRYCAYEIMALHGVLHS IQ            IQETANQAAELLR LGKDI
Sbjct: 301 GEYVKVGAVLRYCAYEIMALHGVLHSRIQAPYKLRITFKSEIQETANQAAELLRGLGKDI 360

Query: 361 NNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTCDSLDLSSKLQSKLLTASSNRLYD 420
           NNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTCDSLDLSSKLQSKL TA SN LYD
Sbjct: 361 NNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTCDSLDLSSKLQSKLSTAPSNSLYD 420

Query: 421 LPSLLAELNGNGPERSLIQQNSHGVAAGTQAEAYVELVRKQSRRQHSWPLREMDVFDDGG 480
           LPSLLAELNGNGPERSLIQQNSHGVAAGTQAEAYVELVRKQSRRQHSWPLREMDVFDDGG
Sbjct: 421 LPSLLAELNGNGPERSLIQQNSHGVAAGTQAEAYVELVRKQSRRQHSWPLREMDVFDDGG 480

Query: 481 GVPIEYLQRMRKQESTAAMSLANFTSLLIEFVARLDYLVETVDELSKMAKFKDNIIS 494
           GVP+E+LQRMRKQESTAAMSLANFTSLLIEFVARLDYLVETVDELSKMAKFKDNIIS
Sbjct: 481 GVPVEFLQRMRKQESTAAMSLANFTSLLIEFVARLDYLVETVDELSKMAKFKDNIIS 537

BLAST of Carg02563 vs. NCBI nr
Match: XP_022996317.1 (aluminum-activated malate transporter 9-like [Cucurbita maxima])

HSP 1 Score: 887.5 bits (2292), Expect = 5.3e-254
Identity = 471/539 (87.38%), Postives = 478/539 (88.68%), Query Frame = 0

Query: 1   MNGKTGNTVIDIDFSTKPEPIGTKAENLAASCKASIGSVLGFCKEDSNRVLFSLKVGLAV 60
           MNGKTGNTVIDIDFSTKPEPIG KAENLAASCKA IGSVLGFCKEDSNRVLFSLKVGLAV
Sbjct: 1   MNGKTGNTVIDIDFSTKPEPIGAKAENLAASCKAPIGSVLGFCKEDSNRVLFSLKVGLAV 60

Query: 61  VLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIA-- 120
           VLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIA  
Sbjct: 61  VLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIAVA 120

Query: 121 ------------------------------LWPSLVPYEYGFRVILFTYCLIVVSGYRMG 180
                                         LWPSLVPYEYGFRV+LFTYCLIVVSGYRMG
Sbjct: 121 QLALRTGPIAEPIIIGISIFLVGSVTSFMKLWPSLVPYEYGFRVLLFTYCLIVVSGYRMG 180

Query: 181 NPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHEDLVATFNSLADSLQECVNKYLE 240
           NPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLH+DLVATFNSLADSLQECV+KYLE
Sbjct: 181 NPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHKDLVATFNSLADSLQECVDKYLE 240

Query: 241 DEGS--NIGQQLPKAVMDEFPDEPAYKKCKATLNSSAKFEALATSAKWEPPHGRFGHFFY 300
           DEGS  NIGQQLPKAVMDEFP+EPAYKKCKATLNSSAKFEALATSAKWEPPHGRF HFFY
Sbjct: 241 DEGSVHNIGQQLPKAVMDEFPEEPAYKKCKATLNSSAKFEALATSAKWEPPHGRFRHFFY 300

Query: 301 PWGEYVKVGAVLRYCAYEIMALHGVLHSPIQ------------IQETANQAAELLRSLGK 360
           PW EYVKVGAVLRYCAYEIMALHGVLHS IQ            IQETANQAAELLRSLGK
Sbjct: 301 PWTEYVKVGAVLRYCAYEIMALHGVLHSRIQAPYKLRITFKSEIQETANQAAELLRSLGK 360

Query: 361 DINNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTCDSLDLSSKLQSKLLTASSNRL 420
           DINNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTC SL LSSKLQSKL TASSN L
Sbjct: 361 DINNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTCASLHLSSKLQSKLSTASSNSL 420

Query: 421 YDLPSLLAELNGNGPERSLIQQNSHGVAAGTQAEAYVELVRKQSRRQHSWPLREMDVFDD 480
           YDLPSLLAELNGNGPERSL QQNSHGVAAGTQAEAYVE VRKQSRRQ+SWPLREMDVFDD
Sbjct: 421 YDLPSLLAELNGNGPERSLNQQNSHGVAAGTQAEAYVEFVRKQSRRQNSWPLREMDVFDD 480

Query: 481 GGGVPIEYLQRMRKQESTAAMSLANFTSLLIEFVARLDYLVETVDELSKMAKFKDNIIS 494
            GGVP+E+LQRMRK ESTAA SLANFTSLLIEFVARLDYLVETVDELSKMAKFKD IIS
Sbjct: 481 DGGVPVEFLQRMRKLESTAATSLANFTSLLIEFVARLDYLVETVDELSKMAKFKDIIIS 539

BLAST of Carg02563 vs. NCBI nr
Match: XP_023532729.1 (aluminum-activated malate transporter 9-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 867.5 bits (2240), Expect = 5.7e-248
Identity = 463/539 (85.90%), Postives = 467/539 (86.64%), Query Frame = 0

Query: 1   MNGKTGNTVIDIDFSTKPEPIGTKAENLAASCKASIGSVLGFCKEDSNRVLFSLKVGLAV 60
           MNGKTGN VIDIDFSTKPEP+G KA NLAASCKASIGSVLGFCKED NRVLFSLKVGLAV
Sbjct: 1   MNGKTGNIVIDIDFSTKPEPLGAKAGNLAASCKASIGSVLGFCKEDRNRVLFSLKVGLAV 60

Query: 61  VLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIA-- 120
           VLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIA  
Sbjct: 61  VLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIAVA 120

Query: 121 ------------------------------LWPSLVPYEYGFRVILFTYCLIVVSGYRMG 180
                                         LWPSLVPYEYGFRVILFTYCLIVVSGYRMG
Sbjct: 121 QLALRTGPIAEPIIIGITIFLVGSVTSFMKLWPSLVPYEYGFRVILFTYCLIVVSGYRMG 180

Query: 181 NPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHEDLVATFNSLADSLQECVNKYLE 240
           NPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLH+DLVATFNSLADSLQECVNKYLE
Sbjct: 181 NPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHKDLVATFNSLADSLQECVNKYLE 240

Query: 241 DEGS--NIGQQLPKAVMDEFPDEPAYKKCKATLNSSAKFEALATSAKWEPPHGRFGHFFY 300
           DEGS  NIGQQLPKAVMDEFPDEPAYKKCKATLNSSAKFEALATSAKWEPPHGRF HFFY
Sbjct: 241 DEGSVHNIGQQLPKAVMDEFPDEPAYKKCKATLNSSAKFEALATSAKWEPPHGRFRHFFY 300

Query: 301 PWGEYVKVGAVLRYCAYEIMALHGVLHSPIQ------------IQETANQAAELLRSLGK 360
           PW EYVKVGAVLRYCAYEIMALHGVLHSPIQ            IQETANQAAELLRSLGK
Sbjct: 301 PWAEYVKVGAVLRYCAYEIMALHGVLHSPIQAPYKLRTTFKSEIQETANQAAELLRSLGK 360

Query: 361 DINNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTCDSLDLSSKLQSKLLTASSNRL 420
           DINNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTCDSLDLSSKLQSKL TASSN L
Sbjct: 361 DINNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTCDSLDLSSKLQSKLSTASSNSL 420

Query: 421 YDLPSLLAELNGNGPERSLIQQNSHGVAAGTQAEAYVELVRKQSRRQHSWPLREMDVFDD 480
           YDLPSLLAE+NGN                GTQAEA VELVRKQSRRQHSWPLREMDVFDD
Sbjct: 421 YDLPSLLAEINGN----------------GTQAEACVELVRKQSRRQHSWPLREMDVFDD 480

Query: 481 GGGVPIEYLQRMRKQESTAAMSLANFTSLLIEFVARLDYLVETVDELSKMAKFKDNIIS 494
           GGGVP+EYLQRMRKQESTAAMSLANFTSLLIEFVARLDYLVETVDELSKMAKFKD IIS
Sbjct: 481 GGGVPVEYLQRMRKQESTAAMSLANFTSLLIEFVARLDYLVETVDELSKMAKFKDKIIS 523

BLAST of Carg02563 vs. ExPASy Swiss-Prot
Match: Q9LPQ8 (Putative aluminum-activated malate transporter 3 OS=Arabidopsis thaliana OX=3702 GN=ALMT3 PE=3 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.1e-68
Identity = 176/500 (35.20%), Postives = 267/500 (53.40%), Query Frame = 0

Query: 46  DSNRVLFSLKVGLAVVLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNR 105
           D  +++FS K+GLA+ L S+LI F+ P      + +WAI+TV ++FE+++GATF++G NR
Sbjct: 88  DPRKMIFSAKMGLALTLTSILIFFKIPGLELSGHYLWAILTVVVIFEFSIGATFSKGCNR 147

Query: 106 ALGSLLAGILAIA-------------------------------LWPSLVPYEYGFRVIL 165
            LG+L AG LA+                                L+P++ PYEYGFRV L
Sbjct: 148 GLGTLSAGGLALGMSWISEMTGNWADVFNAASIFVVAFFATYAKLYPTMKPYEYGFRVFL 207

Query: 166 FTYCLIVVSGYRMGNPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHEDLVATFNS 225
            TYC ++VSGY+ G  + TA+ R   IA+G  V ++VN  ++PIWAGE LH  +   F +
Sbjct: 208 LTYCYVIVSGYKTGEFMETAVSRFLLIALGASVGLIVNTCIYPIWAGEDLHNLVAKNFVN 267

Query: 226 LADSLQECVNKYLEDEGSNIGQQLPKAVM--DEFPDEPAYKKCKATLNSSAKFEALATSA 285
           +A SL+ CVN YLE    +    +P  ++  +   ++P Y   ++ + S+++ + L + A
Sbjct: 268 VATSLEGCVNGYLECVAYD---TIPSRILVYEAVAEDPVYSGYRSAVQSTSQEDTLMSFA 327

Query: 286 KWEPPHGRFGHFFYPWGEYVKVGAVLRYCAYEIMALHGVLHSPIQ------------IQE 345
            WEPPHG +  F YPW  YVKVG  LR+CA  +MALHG + S IQ            +Q 
Sbjct: 328 SWEPPHGPYKSFRYPWALYVKVGGALRHCAIMVMALHGCILSEIQAAEDRRREFRNELQR 387

Query: 346 TANQAAELLRSLGKDINNMQQSLKIY-LLKNVHTAADKLQQALHTHSYPLTPTCDSLDLS 405
              + A++LR +G+ +  M++   I  +L  +H AA++LQ  +   SY L     + ++ 
Sbjct: 388 VGIEGAKVLRYIGESLKKMEKLNPIEDILYEIHQAAEELQSKIDKKSY-LLVNAKNWEIG 447

Query: 406 SKLQSKLLTAS---SNRLYDLPSLLAELNGNGPERSL-IQQNSHGVAAGTQAEAYVELVR 465
           ++ + + LT     SN   DL  +LA  + +  E +L   +N   V       +   L  
Sbjct: 448 NRPRVRDLTDEQKISNLDSDLSRILAHKSQS--EATLRPPKNWDDVTTAANLSSATMLPY 507

Query: 466 KQSR----RQHSWPLREMDVFDDGGGVPIEYLQRMRKQESTAAMSLANFTSLLIEFVARL 492
            QSR    +Q SWP R       G  +     +  +  ES + +SLA F SLLIEFVARL
Sbjct: 508 LQSRTMIHKQPSWPSRIS--ITPGSMLQPPLGEPGKMYESASNLSLATFASLLIEFVARL 567

BLAST of Carg02563 vs. ExPASy Swiss-Prot
Match: Q9LS46 (Aluminum-activated malate transporter 9 OS=Arabidopsis thaliana OX=3702 GN=ALMT9 PE=2 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.1e-68
Identity = 172/506 (33.99%), Postives = 266/506 (52.57%), Query Frame = 0

Query: 46  DSNRVLFSLKVGLAVVLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNR 105
           D  +++FS K+GLA+ +V+LLI ++ P        +WAI+TV ++FE+T+GAT ++GFNR
Sbjct: 84  DPRKIVFSAKIGLALTIVALLIFYQEPNPDLSRYSVWAILTVVVVFEFTIGATLSKGFNR 143

Query: 106 ALGSLLAGILAIA-------------------------------LWPSLVPYEYGFRVIL 165
           ALG+L AG LA+                                L+PS+  YEYGFRV L
Sbjct: 144 ALGTLSAGGLALGMAELSTLFGDWEEIFCTLSIFCIGFLATFMKLYPSMKAYEYGFRVFL 203

Query: 166 FTYCLIVVSGYRMGNPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHEDLVATFNS 225
            TYC I++SG+R G  +  A+ R   IA+G  V++ VN+ ++PIWAGE LH  +V  F +
Sbjct: 204 LTYCYILISGFRTGQFIEVAISRFLLIALGAGVSLGVNMFIYPIWAGEDLHNLVVKNFMN 263

Query: 226 LADSLQECVNKYLEDEGSNIGQQLPKAVMD-EFPDEPAYKKCKATLNSSAKFEALATSAK 285
           +A SL+ CVN YL        +++P  ++  +  ++P YK  ++ + S+++ E+L + A 
Sbjct: 264 VATSLEGCVNGYLR---CLEYERIPSKILTYQASEDPVYKGYRSAVESTSQEESLMSFAI 323

Query: 286 WEPPHGRFGHFFYPWGEYVKVGAVLRYCAYEIMALHGVLHSPIQ------------IQET 345
           WEPPHG +  F YPW  YVK+   L++CA+ +MALHG + S IQ            +Q  
Sbjct: 324 WEPPHGPYKSFNYPWKNYVKLSGALKHCAFTVMALHGCILSEIQAPEERRQVFRQELQRV 383

Query: 346 ANQAAELLRSLGKDINNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPT-CDSLDLSS 405
             + A+LLR LG+ +  M++   + LL  VH AA++LQ  +   SY L  + C  +   +
Sbjct: 384 GVEGAKLLRELGEKVKKMEKLGPVDLLFEVHLAAEELQHKIDKKSYLLVNSECWEIGNRA 443

Query: 406 KLQSKLLTASSNRLYDLPSLLAELNGNGPERSLIQQNSHGVAAGTQAEAYVELVRKQSRR 465
             +S+            P  L  L  + P  +         A  + +EA +E+      +
Sbjct: 444 TKESE------------PQELLSLEDSDPPEN---HAPPIYAFKSLSEAVLEIPPSWGEK 503

Query: 466 QH--------------SWPLREM---DVFDDGGGVPIEYLQRMRKQESTAAMSLANFTSL 490
            H              SWP R +    +    G  P+  ++  +  ES +A+SLA F SL
Sbjct: 504 NHREALNHRPTFSKQVSWPARLVLPPHLETTNGASPL--VETTKTYESASALSLATFASL 563

BLAST of Carg02563 vs. ExPASy Swiss-Prot
Match: Q9C6L8 (Aluminum-activated malate transporter 4 OS=Arabidopsis thaliana OX=3702 GN=ALMT4 PE=3 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.5e-65
Identity = 167/520 (32.12%), Postives = 272/520 (52.31%), Query Frame = 0

Query: 25  AENLAASCKA--SIGSVL-GFCKEDSNRVLFSLKVGLAVVLVSLLILFRAPYDVFGTNII 84
           ++ + AS KA   IG+ L    + D  +V FS+K+G+A+ L S +I  + P        +
Sbjct: 40  SDGITASWKALYDIGAKLYEMGRSDRRKVYFSVKMGMALALCSFVIYLKEPLRDASKYAV 99

Query: 85  WAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIA----------------------- 144
           WAI+TV ++FEY++GAT  +GFNRA+G+L AG LA+                        
Sbjct: 100 WAILTVVVVFEYSIGATLVKGFNRAIGTLSAGGLALGIARLSVSAGEFEELIIIISIFIA 159

Query: 145 --------LWPSLVPYEYGFRVILFTYCLIVVSGYRMGNPLRTAMDRLYSIAIGGFVAVL 204
                   L+P++  YEY FRV L TYC+++VSG    +   TA  R   I +G  + + 
Sbjct: 160 GFSASYLKLYPAMKSYEYAFRVFLLTYCIVLVSGNNSRDFFSTAYYRFLLILVGAGICLG 219

Query: 205 VNVLVFPIWAGEQLHEDLVATFNSLADSLQECVNKYLEDEGSNIGQQLPKAVMD-EFPDE 264
           VN+ + PIWAGE LH+ +V  F S+A+SL+ CVN YL+       +++P  ++  +  D+
Sbjct: 220 VNIFILPIWAGEDLHKLVVKNFKSVANSLEGCVNGYLQCVEY---ERIPSKILTYQASDD 279

Query: 265 PAYKKCKATLNSSAKFEALATSAKWEPPHGRFGHFFYPWGEYVKVGAVLRYCAYEIMALH 324
           P Y   ++ + S+++ ++L   A WEPPHG +  F +PW  YVK+   +R+CA+ +MA+H
Sbjct: 280 PLYSGYRSVVQSTSQEDSLLDFAVWEPPHGPYKTFHHPWANYVKLSGAVRHCAFMVMAMH 339

Query: 325 GVLHSPIQ------------IQETANQAAELLRSLGKDINNMQQSLKIYLLKNVHTAADK 384
           G + S IQ            +Q   N+ A++LR  G+ +  M++     +LK+V  AA++
Sbjct: 340 GCILSEIQAAPEKRQAFRQELQRVGNEGAKVLRLFGEKVEKMEKLSPGNVLKDVQRAAEE 399

Query: 385 LQQALHTHSYPLTPTCDSLDLSSKLQSKLLTASSNRLYDLPSLLAEL------NGNGPER 444
           LQ  + ++S+ L  +     +  K +++    + +   D  S + +       N N P  
Sbjct: 400 LQMKIDSNSFLLVNSESWAAMKEKAEAEEAQQNYHEAKDDESKVIQSLSQIWDNNNNPH- 459

Query: 445 SLIQQNSHGVAAGTQAEAYVELVRKQSRRQHSWPLREMDVFDDGGGVPIEYLQRMRKQES 492
               QN H   AG  ++ ++       R + +WP     V   GG +  E   ++   ES
Sbjct: 460 ---HQNQH---AGNDSQLWISTESMMLRNRENWP----SVSFIGGSMINEIESKV--YES 519

BLAST of Carg02563 vs. ExPASy Swiss-Prot
Match: Q9SHM1 (Aluminum-activated malate transporter 6 OS=Arabidopsis thaliana OX=3702 GN=ALMT6 PE=3 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.6e-64
Identity = 158/504 (31.35%), Postives = 259/504 (51.39%), Query Frame = 0

Query: 32  CKASIGSVLGFCKEDSNRVLFSLKVGLAVVLVSLLILFRAPYDVFGTNIIWAIITVAIMF 91
           C   I ++      D  R+ F++K+G+A+ L S++I  + P        +W I+TV ++F
Sbjct: 22  CFRKITNLCELGHSDRRRIFFAVKMGMALALCSVVIFLKEPLHDASKYSVWGILTVVVVF 81

Query: 92  EYTVGATFNRGFNRALGSLLAGILAIA-------------------------------LW 151
           EY+VGAT  +GFNRA+G++ AG LA+                                L 
Sbjct: 82  EYSVGATLVKGFNRAIGTVSAGGLALGIARLSVLSRDFEQTIIITCIFLAGFIASYSKLH 141

Query: 152 PSLVPYEYGFRVILFTYCLIVVSGYRMGNPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWA 211
           P++ PYEY FRV L T+C+++VSG   G+   TA  R   I +G    ++VN+ +FPIWA
Sbjct: 142 PAMKPYEYAFRVFLLTFCIVLVSGNNTGDFFSTAYYRFLFIVVGATTCLVVNIFIFPIWA 201

Query: 212 GEQLHEDLVATFNSLADSLQECVNKYLEDEGSNIGQQLPKAVMD-EFPDEPAYKKCKATL 271
           GE LH+ +   F S+A+SL+ CVN YL+       +++P  ++  +  D+P Y   ++ +
Sbjct: 202 GEDLHKLVANNFKSVANSLEGCVNGYLQCVEY---ERVPSKILTYQTSDDPLYSGYRSAI 261

Query: 272 NSSAKFEALATSAKWEPPHGRFGHFFYPWGEYVKVGAVLRYCAYEIMALHGVLHSPIQ-- 331
            S+ + E+L   A WEPPHG +  F +PW  YVK+   +R+CA+ +MA+HG + S IQ  
Sbjct: 262 QSTNQEESLLDFAIWEPPHGPYRTFNHPWKNYVKLSGAVRHCAFTVMAIHGCILSEIQAA 321

Query: 332 ----------IQETANQAAELLRSLGKDINNMQQSLKIYLLKNVHTAADKLQQALHTHSY 391
                     +Q   N+ A++LR +G+ +  M+      +L +V  AA++LQ  + + SY
Sbjct: 322 PEKRQAFRHELQRVGNEGAKVLRLIGEKVEKMENLGPGEILNDVQRAAEELQMKIDSKSY 381

Query: 392 PLTPTCDSLDLSSKLQSKLLTASSNRLYDLPSLLAELNGNGPERSLIQQNSHGVAAGTQA 451
            L  +        K +++     ++    + SL    + N         N +  +   ++
Sbjct: 382 LLVNSESWAATKEKAEAEEYEEEAHETKVIKSLSQIWDTNSS-----SNNQNPASGNDES 441

Query: 452 EAYVELVRKQSRRQHSWPLREMDVFDDGGGVPIEYLQRMRKQESTAAMSLANFTSLLIEF 492
           + +        R + +WP     V   GG V  E + ++   ES +++SLA F SLLIEF
Sbjct: 442 QIWESTESMMLRNRETWP----SVSFIGGSVVNETVYKV--YESASSLSLATFASLLIEF 501

BLAST of Carg02563 vs. ExPASy Swiss-Prot
Match: Q93Z29 (Aluminum-activated malate transporter 5 OS=Arabidopsis thaliana OX=3702 GN=ALMT5 PE=1 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 2.6e-62
Identity = 159/496 (32.06%), Postives = 261/496 (52.62%), Query Frame = 0

Query: 46  DSNRVLFSLKVGLAVVLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNR 105
           D  ++ FS+K+G+A+ L S +I  + P        +WAI+TV ++FEY VGAT  +GFNR
Sbjct: 53  DRRKLYFSIKMGIALALCSFVIFLKEPLQDASKFAVWAILTVVLIFEYYVGATLVKGFNR 112

Query: 106 ALGSLLAGILAIA-------------------------------LWPSLVPYEYGFRVIL 165
           ALG++LAG LA+                                L+ S+ PYEY FRV  
Sbjct: 113 ALGTMLAGGLALGVAQLSVLAGEFEEVIIVICIFLAGFGASYLKLYASMKPYEYAFRVFK 172

Query: 166 FTYCLIVVSGYRMGNPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHEDLVATFNS 225
            TYC+++VSG    + L TA  R+  I +G  + +LVNV +FPIWAGE LH+ +   F +
Sbjct: 173 LTYCIVLVSGNNSRDFLSTAYYRILLIGLGATICLLVNVFLFPIWAGEDLHKLVAKNFKN 232

Query: 226 LADSLQECVNKYLEDEGSNIGQQLPKAVMD-EFPDEPAYKKCKATLNSSAKFEALATSAK 285
           +A+SL+ CVN YL+       +++P  ++  +  D+P Y   ++ + S+++ ++L   A 
Sbjct: 233 VANSLEGCVNGYLQCVEY---ERIPSKILTYQASDDPLYSGYRSAVQSTSQEDSLLDFAI 292

Query: 286 WEPPHGRFGHFFYPWGEYVKVGAVLRYCAYEIMALHGVLHSPIQ------------IQET 345
           WEPPHG +  F +PW  YVK+   +R+CA+ +MA+HG + S IQ            ++  
Sbjct: 293 WEPPHGPYKTFNHPWKNYVKLSGAVRHCAFTVMAMHGCILSEIQASPEKRHVFSNELRRV 352

Query: 346 ANQAAELLRSLGKDINNMQQ-SLKI-YLLKNVHTAADKLQQALHTHSYPLTPTCDSLDLS 405
            N+ A++LR  G+ +  M++ SL +  +LK+V  AA+ LQ  + + SY L  +     + 
Sbjct: 353 GNEGAKVLRLFGEKVEKMEKLSLSLGEILKDVQRAAEALQMKIDSKSYLLVNSESWAAIK 412

Query: 406 SKLQSKLLTASSNRLYDLPSLLAELNGNGPERSLIQ----QNSHGVAAGTQAEAYVELVR 465
            + +++    +     D  + +         +SL Q     N++   +  Q++ ++    
Sbjct: 413 EQAEAEEARENDQEAKDDETKVI--------KSLSQIWDTNNNNNHQSNDQSQHWMSTES 472

Query: 466 KQSRRQHSWPLREMDVFDDGGGVPIEYLQRMRKQESTAAMSLANFTSLLIEFVARLDYLV 492
              + +  WP   M   D   G  +  ++  +  ES +++SLA F SLLIEFVARL  +V
Sbjct: 473 MMLKNREMWP--SMSFID---GTVVNEIE-CKVYESASSLSLATFASLLIEFVARLQNIV 531

BLAST of Carg02563 vs. ExPASy TrEMBL
Match: A0A6J1H1V7 (aluminum-activated malate transporter 9-like OS=Cucurbita moschata OX=3662 GN=LOC111459287 PE=3 SV=1)

HSP 1 Score: 925.2 bits (2390), Expect = 1.1e-265
Identity = 484/537 (90.13%), Postives = 487/537 (90.69%), Query Frame = 0

Query: 1   MNGKTGNTVIDIDFSTKPEPIGTKAENLAASCKASIGSVLGFCKEDSNRVLFSLKVGLAV 60
           MNGKTGNTVIDIDFSTKPEPIG KAENLAASCKASIGSVLGFCKEDSNRVLFSLKVGLAV
Sbjct: 1   MNGKTGNTVIDIDFSTKPEPIGAKAENLAASCKASIGSVLGFCKEDSNRVLFSLKVGLAV 60

Query: 61  VLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIA-- 120
           VLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIA  
Sbjct: 61  VLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIAVA 120

Query: 121 ------------------------------LWPSLVPYEYGFRVILFTYCLIVVSGYRMG 180
                                         LWPSLVPYEYGFRVILFTYCLIVVSGYRMG
Sbjct: 121 QLALRTGPIAEPIIIGISIFLVGSVTSFMKLWPSLVPYEYGFRVILFTYCLIVVSGYRMG 180

Query: 181 NPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHEDLVATFNSLADSLQECVNKYLE 240
           NPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLH+DLVATFNSLADSLQECVNKYLE
Sbjct: 181 NPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHKDLVATFNSLADSLQECVNKYLE 240

Query: 241 DEGSNIGQQLPKAVMDEFPDEPAYKKCKATLNSSAKFEALATSAKWEPPHGRFGHFFYPW 300
           DEGSNIGQQLPKAVMDEFPDEPAYKKCKATLNSSAKFEALATSAKWEPPHGRFGHFFYPW
Sbjct: 241 DEGSNIGQQLPKAVMDEFPDEPAYKKCKATLNSSAKFEALATSAKWEPPHGRFGHFFYPW 300

Query: 301 GEYVKVGAVLRYCAYEIMALHGVLHSPIQ------------IQETANQAAELLRSLGKDI 360
           GEYVKVGAVLRYCAYEIMALHGVLHS IQ            IQETANQAAELLR LGKDI
Sbjct: 301 GEYVKVGAVLRYCAYEIMALHGVLHSRIQAPYKLRITFKSEIQETANQAAELLRGLGKDI 360

Query: 361 NNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTCDSLDLSSKLQSKLLTASSNRLYD 420
           NNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTCDSLDLSSKLQSKL TA SN LYD
Sbjct: 361 NNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTCDSLDLSSKLQSKLSTAPSNSLYD 420

Query: 421 LPSLLAELNGNGPERSLIQQNSHGVAAGTQAEAYVELVRKQSRRQHSWPLREMDVFDDGG 480
           LPSLLAELNGNGPERSLIQQNSHGVAAGTQAEAYVELVRKQSRRQHSWPLREMDVFDDGG
Sbjct: 421 LPSLLAELNGNGPERSLIQQNSHGVAAGTQAEAYVELVRKQSRRQHSWPLREMDVFDDGG 480

Query: 481 GVPIEYLQRMRKQESTAAMSLANFTSLLIEFVARLDYLVETVDELSKMAKFKDNIIS 494
           GVP+E+LQRMRKQESTAAMSLANFTSLLIEFVARLDYLVETVDELSKMAKFKDNIIS
Sbjct: 481 GVPVEFLQRMRKQESTAAMSLANFTSLLIEFVARLDYLVETVDELSKMAKFKDNIIS 537

BLAST of Carg02563 vs. ExPASy TrEMBL
Match: A0A6J1K1K6 (aluminum-activated malate transporter 9-like OS=Cucurbita maxima OX=3661 GN=LOC111491581 PE=3 SV=1)

HSP 1 Score: 887.5 bits (2292), Expect = 2.6e-254
Identity = 471/539 (87.38%), Postives = 478/539 (88.68%), Query Frame = 0

Query: 1   MNGKTGNTVIDIDFSTKPEPIGTKAENLAASCKASIGSVLGFCKEDSNRVLFSLKVGLAV 60
           MNGKTGNTVIDIDFSTKPEPIG KAENLAASCKA IGSVLGFCKEDSNRVLFSLKVGLAV
Sbjct: 1   MNGKTGNTVIDIDFSTKPEPIGAKAENLAASCKAPIGSVLGFCKEDSNRVLFSLKVGLAV 60

Query: 61  VLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIA-- 120
           VLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIA  
Sbjct: 61  VLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIAVA 120

Query: 121 ------------------------------LWPSLVPYEYGFRVILFTYCLIVVSGYRMG 180
                                         LWPSLVPYEYGFRV+LFTYCLIVVSGYRMG
Sbjct: 121 QLALRTGPIAEPIIIGISIFLVGSVTSFMKLWPSLVPYEYGFRVLLFTYCLIVVSGYRMG 180

Query: 181 NPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHEDLVATFNSLADSLQECVNKYLE 240
           NPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLH+DLVATFNSLADSLQECV+KYLE
Sbjct: 181 NPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHKDLVATFNSLADSLQECVDKYLE 240

Query: 241 DEGS--NIGQQLPKAVMDEFPDEPAYKKCKATLNSSAKFEALATSAKWEPPHGRFGHFFY 300
           DEGS  NIGQQLPKAVMDEFP+EPAYKKCKATLNSSAKFEALATSAKWEPPHGRF HFFY
Sbjct: 241 DEGSVHNIGQQLPKAVMDEFPEEPAYKKCKATLNSSAKFEALATSAKWEPPHGRFRHFFY 300

Query: 301 PWGEYVKVGAVLRYCAYEIMALHGVLHSPIQ------------IQETANQAAELLRSLGK 360
           PW EYVKVGAVLRYCAYEIMALHGVLHS IQ            IQETANQAAELLRSLGK
Sbjct: 301 PWTEYVKVGAVLRYCAYEIMALHGVLHSRIQAPYKLRITFKSEIQETANQAAELLRSLGK 360

Query: 361 DINNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTCDSLDLSSKLQSKLLTASSNRL 420
           DINNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTC SL LSSKLQSKL TASSN L
Sbjct: 361 DINNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTCASLHLSSKLQSKLSTASSNSL 420

Query: 421 YDLPSLLAELNGNGPERSLIQQNSHGVAAGTQAEAYVELVRKQSRRQHSWPLREMDVFDD 480
           YDLPSLLAELNGNGPERSL QQNSHGVAAGTQAEAYVE VRKQSRRQ+SWPLREMDVFDD
Sbjct: 421 YDLPSLLAELNGNGPERSLNQQNSHGVAAGTQAEAYVEFVRKQSRRQNSWPLREMDVFDD 480

Query: 481 GGGVPIEYLQRMRKQESTAAMSLANFTSLLIEFVARLDYLVETVDELSKMAKFKDNIIS 494
            GGVP+E+LQRMRK ESTAA SLANFTSLLIEFVARLDYLVETVDELSKMAKFKD IIS
Sbjct: 481 DGGVPVEFLQRMRKLESTAATSLANFTSLLIEFVARLDYLVETVDELSKMAKFKDIIIS 539

BLAST of Carg02563 vs. ExPASy TrEMBL
Match: A0A6J1CX80 (aluminum-activated malate transporter 9-like OS=Momordica charantia OX=3673 GN=LOC111015266 PE=3 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 9.6e-193
Identity = 378/540 (70.00%), Postives = 417/540 (77.22%), Query Frame = 0

Query: 1   MNGKTGNTVID-IDFSTKPEP----IGTKAENLAASCKASIGSVLGFCKEDSNRVLFSLK 60
           MNGKT +  ID I F +KPEP       K++NLAAS +ASI S+  FCKEDSNRV FSLK
Sbjct: 1   MNGKTSSIEIDIIGFPSKPEPPTKGSSGKSKNLAASFRASISSIWDFCKEDSNRVKFSLK 60

Query: 61  VGLAVVLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGIL 120
           VGLAVVLVSLLILF+APYD FGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGIL
Sbjct: 61  VGLAVVLVSLLILFQAPYDAFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGIL 120

Query: 121 AIA--------------------------------LWPSLVPYEYGFRVILFTYCLIVVS 180
           AI                                 LWPSL PYEYGFRVILFTYCLIVVS
Sbjct: 121 AIGVAQLALRTGPVAQPIIIGISIFLVGAVTSLMKLWPSLTPYEYGFRVILFTYCLIVVS 180

Query: 181 GYRMGNPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHEDLVATFNSLADSLQECV 240
           GYRMGNPLRTAMDRLY+IAIGGFVAVLVNVLVFPIWAG QLH++LV+TF+S+A+SLQECV
Sbjct: 181 GYRMGNPLRTAMDRLYTIAIGGFVAVLVNVLVFPIWAGHQLHKELVSTFDSVANSLQECV 240

Query: 241 NKYLEDEGSNIGQQLPKAVMDEFPDEPAYKKCKATLNSSAKFEALATSAKWEPPHGRFGH 300
           NKYLE+EGS    QLPKA+MDEFPDEPAYKK KATLNSSAKFE+LATSAKWEPPHGRF  
Sbjct: 241 NKYLEEEGSQ-QNQLPKALMDEFPDEPAYKKFKATLNSSAKFESLATSAKWEPPHGRFRQ 300

Query: 301 FFYPWGEYVKVGAVLRYCAYEIMALHGVLHSPIQ------------IQETANQAAELLRS 360
           FFYPW EYVKVGAVLRYCAYE+MALHG+LHS IQ            IQ+ ANQAAEL+RS
Sbjct: 301 FFYPWAEYVKVGAVLRYCAYEVMALHGLLHSQIQAPYNLRIAFKSEIQDAANQAAELVRS 360

Query: 361 LGKDINNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPT--CDSLDLSSKLQSKLLTA 420
           LG+DI+NM+QSLKI LLK +HT+A+KLQ A+ THSY LTP   CD++D SSKLQSKL T 
Sbjct: 361 LGEDISNMKQSLKISLLKRLHTSAEKLQLAIDTHSYLLTPATYCDAIDFSSKLQSKLSTT 420

Query: 421 SSNRLYDLPSLLAELNGNGPERSLIQQNSHGVAAGTQAEAYVELVRKQSRRQHSWPLREM 480
           SS+ L+DLP+ L  +                 A   QAEA VEL RKQSRRQHSWP  E+
Sbjct: 421 SSSSLHDLPNELDVV----------------AAGAAQAEACVELGRKQSRRQHSWPSLEV 480

Query: 481 DVFDDGGGVPIEYLQRMRKQESTAAMSLANFTSLLIEFVARLDYLVETVDELSKMAKFKD 490
              D+GG V +E+  RMRK ES+AAMSLANFTSLLIEFVARLDYLVETVDELSKMAKFKD
Sbjct: 481 GALDEGGSVVVEFSPRMRKLESSAAMSLANFTSLLIEFVARLDYLVETVDELSKMAKFKD 523

BLAST of Carg02563 vs. ExPASy TrEMBL
Match: A0A0A0KMG7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G151600 PE=3 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.6e-187
Identity = 371/538 (68.96%), Postives = 410/538 (76.21%), Query Frame = 0

Query: 1   MNGKTGNTVIDIDFSTKPEPIGTKAENLAASCKASIG--SVLGFCKEDSNRVLFSLKVGL 60
           MN KTG+ VIDI   TK EP+  K+     +    +G  SV  FCKED NR+LFS KVGL
Sbjct: 1   MNEKTGSIVIDI-IETKLEPLWAKSGGKLGNSSTLLGIRSVWNFCKEDRNRLLFSFKVGL 60

Query: 61  AVVLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIA 120
           AVVLVSLLIL +APYDVFG+NIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAI 
Sbjct: 61  AVVLVSLLILLQAPYDVFGSNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIG 120

Query: 121 L--------------------------------WPSLVPYEYGFRVILFTYCLIVVSGYR 180
           +                                WP L PYEYGFRVILFTYCLIVVSGYR
Sbjct: 121 VAQLALLTGPVGEPIVIGISIFLVGSITTLMKQWPRLTPYEYGFRVILFTYCLIVVSGYR 180

Query: 181 MGNPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHEDLVATFNSLADSLQECVNKY 240
           MGNPLR A+DRLYSIAIG  VAVLVNVLVFPIWAG QLH DLV  FNS+ADSLQECV KY
Sbjct: 181 MGNPLRIAIDRLYSIAIGALVAVLVNVLVFPIWAGHQLHNDLVNAFNSIADSLQECVKKY 240

Query: 241 LED-EGSNIGQQLP-KAVMDEFPDEPAYKKCKATLNSSAKFEALATSAKWEPPHGRFGHF 300
           LED E  NI Q++P KAVMDEFPDEPAYKKCKATLNSS+KF+ LATSAKWEPPHGRF  F
Sbjct: 241 LEDEEWKNIDQEVPLKAVMDEFPDEPAYKKCKATLNSSSKFDTLATSAKWEPPHGRFKQF 300

Query: 301 FYPWGEYVKVGAVLRYCAYEIMALHGVLHSPIQ------------IQETANQAAELLRSL 360
           FYPW EYVKVGAVLRYCAY++MALHGVLHS IQ            IQ+ ANQAAEL+RSL
Sbjct: 301 FYPWTEYVKVGAVLRYCAYQVMALHGVLHSQIQAPYNLRITFKSEIQDVANQAAELMRSL 360

Query: 361 GKDINNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTCDSLDLSSKLQSKLLTASSN 420
           GKDI+NM+QS+KI  LKNVH+ A+KLQ+A+ THSY LTPTC+++ LS        T SS+
Sbjct: 361 GKDIDNMKQSIKISHLKNVHSTAEKLQRAIDTHSYLLTPTCETIGLS--------TTSSS 420

Query: 421 RLYDLPSLLAELNGNGPERSLIQQNSHGVAAGTQAEAYVELVRK-QSRRQHSWPLREMDV 480
            L +LPSL AEL+ NG +RSL +Q+SHG          V+LVRK QSRRQHSWPLREMDV
Sbjct: 421 NLDNLPSLSAELDSNGSKRSLNKQDSHG----------VDLVRKQQSRRQHSWPLREMDV 480

Query: 481 FDDGGGVPIEYLQRMRKQESTAAMSLANFTSLLIEFVARLDYLVETVDELSKMAKFKD 490
           FDDG  V IE+L RMRK ESTAAMSLANFTSLLIEFVARLDYLVETVDELS+MAKF +
Sbjct: 481 FDDGRCVAIEFLPRMRKLESTAAMSLANFTSLLIEFVARLDYLVETVDELSRMAKFNE 519

BLAST of Carg02563 vs. ExPASy TrEMBL
Match: A0A4U5PLY7 (Uncharacterized protein OS=Populus alba OX=43335 GN=D5086_0000211980 PE=3 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 4.8e-176
Identity = 347/538 (64.50%), Postives = 401/538 (74.54%), Query Frame = 0

Query: 1   MNGKTGNTVIDI-DFSTKPEPIGTKAE---NLAASCKASIGSVLGFCKEDSNRVLFSLKV 60
           MNGK G+  I+I   +TK +  GT  E      +SCKA I SV  F KEDSNRV F+LKV
Sbjct: 1   MNGKKGSFEINIPPEATKSKLPGTSKEGSIGAFSSCKAWIWSVWEFAKEDSNRVKFALKV 60

Query: 61  GLAVVLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNRALGSLLAGILA 120
           GLAV+LVSLLILFRAPYD+FGTNIIW+I+TVAIMFEYTVGATFNRGFNRALGSLLAG+LA
Sbjct: 61  GLAVLLVSLLILFRAPYDIFGTNIIWSILTVAIMFEYTVGATFNRGFNRALGSLLAGVLA 120

Query: 121 IA--------------------------------LWPSLVPYEYGFRVILFTYCLIVVSG 180
           IA                                LWPSLVPYEYGFRVILFTYCLI+VSG
Sbjct: 121 IAVAQLAIQSGRVAEPIIIGISIFLIGSITSFMKLWPSLVPYEYGFRVILFTYCLIIVSG 180

Query: 181 YRMGNPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHEDLVATFNSLADSLQECVN 240
           YRMGNP+ TAMDRLYSIAIGGFVAVLVNV VFPIWAGEQLH++LV +FNS+ADSL+ECV 
Sbjct: 181 YRMGNPITTAMDRLYSIAIGGFVAVLVNVFVFPIWAGEQLHKELVNSFNSVADSLEECVK 240

Query: 241 KYLEDEGSNIGQQLPKAVMDEFPDEPAYKKCKATLNSSAKFEALATSAKWEPPHGRFGHF 300
           KYLEDEG +   +  K VMDEFPDEP Y++CK+TLNSSAK E+LA SAKWEPPHG+FGHF
Sbjct: 241 KYLEDEGLD-HPEFSKTVMDEFPDEPNYRRCKSTLNSSAKLESLANSAKWEPPHGKFGHF 300

Query: 301 FYPWGEYVKVGAVLRYCAYEIMALHGVLHSPIQ------------IQETANQAAELLRSL 360
           FYPW EYVKVGAVLRYCAYE+MALHGVLHS IQ            IQE A  AAEL+RSL
Sbjct: 301 FYPWSEYVKVGAVLRYCAYEVMALHGVLHSEIQAPHNLRFTFYSEIQEAATHAAELVRSL 360

Query: 361 GKDINNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPTCDSLDLSSKLQSKLLTASSN 420
           GKDI+NM++S K  LLK VH+A ++LQ+A+  HSY LT   D  D SSK  +KL    S 
Sbjct: 361 GKDISNMKRSPKTSLLKKVHSATERLQRAIDMHSYLLTSNFDPPDNSSKPLTKLPVTFST 420

Query: 421 RLYDLPSLLAELNGNGPERSLIQQNSHGVAAGT---QAEAYVELVRKQSRRQHSWPLREM 480
             YDL + L E + N  E +L Q N + + +GT   Q E+Y E++RKQSRR HSWP RE+
Sbjct: 421 TQYDLSNPLTEFDSNSTENNLSQINEN-MPSGTPPQQTESYHEMMRKQSRRLHSWPSREV 480

Query: 481 DVFDDGGGVPIEYLQRMRKQESTAAMSLANFTSLLIEFVARLDYLVETVDELSKMAKF 488
           D F++ GG+ ++ L RM+  ESTAA+SLANFTSLLIEFVARLD+LVE VDELSKMAKF
Sbjct: 481 DAFEEEGGLSMDLLPRMKALESTAALSLANFTSLLIEFVARLDHLVEAVDELSKMAKF 536

BLAST of Carg02563 vs. TAIR 10
Match: AT1G18420.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 262.3 bits (669), Expect = 7.8e-70
Identity = 176/500 (35.20%), Postives = 267/500 (53.40%), Query Frame = 0

Query: 46  DSNRVLFSLKVGLAVVLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNR 105
           D  +++FS K+GLA+ L S+LI F+ P      + +WAI+TV ++FE+++GATF++G NR
Sbjct: 88  DPRKMIFSAKMGLALTLTSILIFFKIPGLELSGHYLWAILTVVVIFEFSIGATFSKGCNR 147

Query: 106 ALGSLLAGILAIA-------------------------------LWPSLVPYEYGFRVIL 165
            LG+L AG LA+                                L+P++ PYEYGFRV L
Sbjct: 148 GLGTLSAGGLALGMSWISEMTGNWADVFNAASIFVVAFFATYAKLYPTMKPYEYGFRVFL 207

Query: 166 FTYCLIVVSGYRMGNPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHEDLVATFNS 225
            TYC ++VSGY+ G  + TA+ R   IA+G  V ++VN  ++PIWAGE LH  +   F +
Sbjct: 208 LTYCYVIVSGYKTGEFMETAVSRFLLIALGASVGLIVNTCIYPIWAGEDLHNLVAKNFVN 267

Query: 226 LADSLQECVNKYLEDEGSNIGQQLPKAVM--DEFPDEPAYKKCKATLNSSAKFEALATSA 285
           +A SL+ CVN YLE    +    +P  ++  +   ++P Y   ++ + S+++ + L + A
Sbjct: 268 VATSLEGCVNGYLECVAYD---TIPSRILVYEAVAEDPVYSGYRSAVQSTSQEDTLMSFA 327

Query: 286 KWEPPHGRFGHFFYPWGEYVKVGAVLRYCAYEIMALHGVLHSPIQ------------IQE 345
            WEPPHG +  F YPW  YVKVG  LR+CA  +MALHG + S IQ            +Q 
Sbjct: 328 SWEPPHGPYKSFRYPWALYVKVGGALRHCAIMVMALHGCILSEIQAAEDRRREFRNELQR 387

Query: 346 TANQAAELLRSLGKDINNMQQSLKIY-LLKNVHTAADKLQQALHTHSYPLTPTCDSLDLS 405
              + A++LR +G+ +  M++   I  +L  +H AA++LQ  +   SY L     + ++ 
Sbjct: 388 VGIEGAKVLRYIGESLKKMEKLNPIEDILYEIHQAAEELQSKIDKKSY-LLVNAKNWEIG 447

Query: 406 SKLQSKLLTAS---SNRLYDLPSLLAELNGNGPERSL-IQQNSHGVAAGTQAEAYVELVR 465
           ++ + + LT     SN   DL  +LA  + +  E +L   +N   V       +   L  
Sbjct: 448 NRPRVRDLTDEQKISNLDSDLSRILAHKSQS--EATLRPPKNWDDVTTAANLSSATMLPY 507

Query: 466 KQSR----RQHSWPLREMDVFDDGGGVPIEYLQRMRKQESTAAMSLANFTSLLIEFVARL 492
            QSR    +Q SWP R       G  +     +  +  ES + +SLA F SLLIEFVARL
Sbjct: 508 LQSRTMIHKQPSWPSRIS--ITPGSMLQPPLGEPGKMYESASNLSLATFASLLIEFVARL 567

BLAST of Carg02563 vs. TAIR 10
Match: AT3G18440.1 (aluminum-activated malate transporter 9 )

HSP 1 Score: 262.3 bits (669), Expect = 7.8e-70
Identity = 172/506 (33.99%), Postives = 266/506 (52.57%), Query Frame = 0

Query: 46  DSNRVLFSLKVGLAVVLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNR 105
           D  +++FS K+GLA+ +V+LLI ++ P        +WAI+TV ++FE+T+GAT ++GFNR
Sbjct: 84  DPRKIVFSAKIGLALTIVALLIFYQEPNPDLSRYSVWAILTVVVVFEFTIGATLSKGFNR 143

Query: 106 ALGSLLAGILAIA-------------------------------LWPSLVPYEYGFRVIL 165
           ALG+L AG LA+                                L+PS+  YEYGFRV L
Sbjct: 144 ALGTLSAGGLALGMAELSTLFGDWEEIFCTLSIFCIGFLATFMKLYPSMKAYEYGFRVFL 203

Query: 166 FTYCLIVVSGYRMGNPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHEDLVATFNS 225
            TYC I++SG+R G  +  A+ R   IA+G  V++ VN+ ++PIWAGE LH  +V  F +
Sbjct: 204 LTYCYILISGFRTGQFIEVAISRFLLIALGAGVSLGVNMFIYPIWAGEDLHNLVVKNFMN 263

Query: 226 LADSLQECVNKYLEDEGSNIGQQLPKAVMD-EFPDEPAYKKCKATLNSSAKFEALATSAK 285
           +A SL+ CVN YL        +++P  ++  +  ++P YK  ++ + S+++ E+L + A 
Sbjct: 264 VATSLEGCVNGYLR---CLEYERIPSKILTYQASEDPVYKGYRSAVESTSQEESLMSFAI 323

Query: 286 WEPPHGRFGHFFYPWGEYVKVGAVLRYCAYEIMALHGVLHSPIQ------------IQET 345
           WEPPHG +  F YPW  YVK+   L++CA+ +MALHG + S IQ            +Q  
Sbjct: 324 WEPPHGPYKSFNYPWKNYVKLSGALKHCAFTVMALHGCILSEIQAPEERRQVFRQELQRV 383

Query: 346 ANQAAELLRSLGKDINNMQQSLKIYLLKNVHTAADKLQQALHTHSYPLTPT-CDSLDLSS 405
             + A+LLR LG+ +  M++   + LL  VH AA++LQ  +   SY L  + C  +   +
Sbjct: 384 GVEGAKLLRELGEKVKKMEKLGPVDLLFEVHLAAEELQHKIDKKSYLLVNSECWEIGNRA 443

Query: 406 KLQSKLLTASSNRLYDLPSLLAELNGNGPERSLIQQNSHGVAAGTQAEAYVELVRKQSRR 465
             +S+            P  L  L  + P  +         A  + +EA +E+      +
Sbjct: 444 TKESE------------PQELLSLEDSDPPEN---HAPPIYAFKSLSEAVLEIPPSWGEK 503

Query: 466 QH--------------SWPLREM---DVFDDGGGVPIEYLQRMRKQESTAAMSLANFTSL 490
            H              SWP R +    +    G  P+  ++  +  ES +A+SLA F SL
Sbjct: 504 NHREALNHRPTFSKQVSWPARLVLPPHLETTNGASPL--VETTKTYESASALSLATFASL 563

BLAST of Carg02563 vs. TAIR 10
Match: AT1G25480.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 251.9 bits (642), Expect = 1.1e-66
Identity = 167/520 (32.12%), Postives = 272/520 (52.31%), Query Frame = 0

Query: 25  AENLAASCKA--SIGSVL-GFCKEDSNRVLFSLKVGLAVVLVSLLILFRAPYDVFGTNII 84
           ++ + AS KA   IG+ L    + D  +V FS+K+G+A+ L S +I  + P        +
Sbjct: 40  SDGITASWKALYDIGAKLYEMGRSDRRKVYFSVKMGMALALCSFVIYLKEPLRDASKYAV 99

Query: 85  WAIITVAIMFEYTVGATFNRGFNRALGSLLAGILAIA----------------------- 144
           WAI+TV ++FEY++GAT  +GFNRA+G+L AG LA+                        
Sbjct: 100 WAILTVVVVFEYSIGATLVKGFNRAIGTLSAGGLALGIARLSVSAGEFEELIIIISIFIA 159

Query: 145 --------LWPSLVPYEYGFRVILFTYCLIVVSGYRMGNPLRTAMDRLYSIAIGGFVAVL 204
                   L+P++  YEY FRV L TYC+++VSG    +   TA  R   I +G  + + 
Sbjct: 160 GFSASYLKLYPAMKSYEYAFRVFLLTYCIVLVSGNNSRDFFSTAYYRFLLILVGAGICLG 219

Query: 205 VNVLVFPIWAGEQLHEDLVATFNSLADSLQECVNKYLEDEGSNIGQQLPKAVMD-EFPDE 264
           VN+ + PIWAGE LH+ +V  F S+A+SL+ CVN YL+       +++P  ++  +  D+
Sbjct: 220 VNIFILPIWAGEDLHKLVVKNFKSVANSLEGCVNGYLQCVEY---ERIPSKILTYQASDD 279

Query: 265 PAYKKCKATLNSSAKFEALATSAKWEPPHGRFGHFFYPWGEYVKVGAVLRYCAYEIMALH 324
           P Y   ++ + S+++ ++L   A WEPPHG +  F +PW  YVK+   +R+CA+ +MA+H
Sbjct: 280 PLYSGYRSVVQSTSQEDSLLDFAVWEPPHGPYKTFHHPWANYVKLSGAVRHCAFMVMAMH 339

Query: 325 GVLHSPIQ------------IQETANQAAELLRSLGKDINNMQQSLKIYLLKNVHTAADK 384
           G + S IQ            +Q   N+ A++LR  G+ +  M++     +LK+V  AA++
Sbjct: 340 GCILSEIQAAPEKRQAFRQELQRVGNEGAKVLRLFGEKVEKMEKLSPGNVLKDVQRAAEE 399

Query: 385 LQQALHTHSYPLTPTCDSLDLSSKLQSKLLTASSNRLYDLPSLLAEL------NGNGPER 444
           LQ  + ++S+ L  +     +  K +++    + +   D  S + +       N N P  
Sbjct: 400 LQMKIDSNSFLLVNSESWAAMKEKAEAEEAQQNYHEAKDDESKVIQSLSQIWDNNNNPH- 459

Query: 445 SLIQQNSHGVAAGTQAEAYVELVRKQSRRQHSWPLREMDVFDDGGGVPIEYLQRMRKQES 492
               QN H   AG  ++ ++       R + +WP     V   GG +  E   ++   ES
Sbjct: 460 ---HQNQH---AGNDSQLWISTESMMLRNRENWP----SVSFIGGSMINEIESKV--YES 519

BLAST of Carg02563 vs. TAIR 10
Match: AT2G17470.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 248.4 bits (633), Expect = 1.2e-65
Identity = 158/504 (31.35%), Postives = 259/504 (51.39%), Query Frame = 0

Query: 32  CKASIGSVLGFCKEDSNRVLFSLKVGLAVVLVSLLILFRAPYDVFGTNIIWAIITVAIMF 91
           C   I ++      D  R+ F++K+G+A+ L S++I  + P        +W I+TV ++F
Sbjct: 22  CFRKITNLCELGHSDRRRIFFAVKMGMALALCSVVIFLKEPLHDASKYSVWGILTVVVVF 81

Query: 92  EYTVGATFNRGFNRALGSLLAGILAIA-------------------------------LW 151
           EY+VGAT  +GFNRA+G++ AG LA+                                L 
Sbjct: 82  EYSVGATLVKGFNRAIGTVSAGGLALGIARLSVLSRDFEQTIIITCIFLAGFIASYSKLH 141

Query: 152 PSLVPYEYGFRVILFTYCLIVVSGYRMGNPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWA 211
           P++ PYEY FRV L T+C+++VSG   G+   TA  R   I +G    ++VN+ +FPIWA
Sbjct: 142 PAMKPYEYAFRVFLLTFCIVLVSGNNTGDFFSTAYYRFLFIVVGATTCLVVNIFIFPIWA 201

Query: 212 GEQLHEDLVATFNSLADSLQECVNKYLEDEGSNIGQQLPKAVMD-EFPDEPAYKKCKATL 271
           GE LH+ +   F S+A+SL+ CVN YL+       +++P  ++  +  D+P Y   ++ +
Sbjct: 202 GEDLHKLVANNFKSVANSLEGCVNGYLQCVEY---ERVPSKILTYQTSDDPLYSGYRSAI 261

Query: 272 NSSAKFEALATSAKWEPPHGRFGHFFYPWGEYVKVGAVLRYCAYEIMALHGVLHSPIQ-- 331
            S+ + E+L   A WEPPHG +  F +PW  YVK+   +R+CA+ +MA+HG + S IQ  
Sbjct: 262 QSTNQEESLLDFAIWEPPHGPYRTFNHPWKNYVKLSGAVRHCAFTVMAIHGCILSEIQAA 321

Query: 332 ----------IQETANQAAELLRSLGKDINNMQQSLKIYLLKNVHTAADKLQQALHTHSY 391
                     +Q   N+ A++LR +G+ +  M+      +L +V  AA++LQ  + + SY
Sbjct: 322 PEKRQAFRHELQRVGNEGAKVLRLIGEKVEKMENLGPGEILNDVQRAAEELQMKIDSKSY 381

Query: 392 PLTPTCDSLDLSSKLQSKLLTASSNRLYDLPSLLAELNGNGPERSLIQQNSHGVAAGTQA 451
            L  +        K +++     ++    + SL    + N         N +  +   ++
Sbjct: 382 LLVNSESWAATKEKAEAEEYEEEAHETKVIKSLSQIWDTNSS-----SNNQNPASGNDES 441

Query: 452 EAYVELVRKQSRRQHSWPLREMDVFDDGGGVPIEYLQRMRKQESTAAMSLANFTSLLIEF 492
           + +        R + +WP     V   GG V  E + ++   ES +++SLA F SLLIEF
Sbjct: 442 QIWESTESMMLRNRETWP----SVSFIGGSVVNETVYKV--YESASSLSLATFASLLIEF 501

BLAST of Carg02563 vs. TAIR 10
Match: AT1G68600.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 241.1 bits (614), Expect = 1.9e-63
Identity = 159/496 (32.06%), Postives = 261/496 (52.62%), Query Frame = 0

Query: 46  DSNRVLFSLKVGLAVVLVSLLILFRAPYDVFGTNIIWAIITVAIMFEYTVGATFNRGFNR 105
           D  ++ FS+K+G+A+ L S +I  + P        +WAI+TV ++FEY VGAT  +GFNR
Sbjct: 53  DRRKLYFSIKMGIALALCSFVIFLKEPLQDASKFAVWAILTVVLIFEYYVGATLVKGFNR 112

Query: 106 ALGSLLAGILAIA-------------------------------LWPSLVPYEYGFRVIL 165
           ALG++LAG LA+                                L+ S+ PYEY FRV  
Sbjct: 113 ALGTMLAGGLALGVAQLSVLAGEFEEVIIVICIFLAGFGASYLKLYASMKPYEYAFRVFK 172

Query: 166 FTYCLIVVSGYRMGNPLRTAMDRLYSIAIGGFVAVLVNVLVFPIWAGEQLHEDLVATFNS 225
            TYC+++VSG    + L TA  R+  I +G  + +LVNV +FPIWAGE LH+ +   F +
Sbjct: 173 LTYCIVLVSGNNSRDFLSTAYYRILLIGLGATICLLVNVFLFPIWAGEDLHKLVAKNFKN 232

Query: 226 LADSLQECVNKYLEDEGSNIGQQLPKAVMD-EFPDEPAYKKCKATLNSSAKFEALATSAK 285
           +A+SL+ CVN YL+       +++P  ++  +  D+P Y   ++ + S+++ ++L   A 
Sbjct: 233 VANSLEGCVNGYLQCVEY---ERIPSKILTYQASDDPLYSGYRSAVQSTSQEDSLLDFAI 292

Query: 286 WEPPHGRFGHFFYPWGEYVKVGAVLRYCAYEIMALHGVLHSPIQ------------IQET 345
           WEPPHG +  F +PW  YVK+   +R+CA+ +MA+HG + S IQ            ++  
Sbjct: 293 WEPPHGPYKTFNHPWKNYVKLSGAVRHCAFTVMAMHGCILSEIQASPEKRHVFSNELRRV 352

Query: 346 ANQAAELLRSLGKDINNMQQ-SLKI-YLLKNVHTAADKLQQALHTHSYPLTPTCDSLDLS 405
            N+ A++LR  G+ +  M++ SL +  +LK+V  AA+ LQ  + + SY L  +     + 
Sbjct: 353 GNEGAKVLRLFGEKVEKMEKLSLSLGEILKDVQRAAEALQMKIDSKSYLLVNSESWAAIK 412

Query: 406 SKLQSKLLTASSNRLYDLPSLLAELNGNGPERSLIQ----QNSHGVAAGTQAEAYVELVR 465
            + +++    +     D  + +         +SL Q     N++   +  Q++ ++    
Sbjct: 413 EQAEAEEARENDQEAKDDETKVI--------KSLSQIWDTNNNNNHQSNDQSQHWMSTES 472

Query: 466 KQSRRQHSWPLREMDVFDDGGGVPIEYLQRMRKQESTAAMSLANFTSLLIEFVARLDYLV 492
              + +  WP   M   D   G  +  ++  +  ES +++SLA F SLLIEFVARL  +V
Sbjct: 473 MMLKNREMWP--SMSFID---GTVVNEIE-CKVYESASSLSLATFASLLIEFVARLQNIV 531

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7036007.14.0e-278100.00putative aluminum-activated malate transporter 3, partial [Cucurbita argyrosperm... [more]
KAG6606059.11.5e-26991.43Aluminum-activated malate transporter 9, partial [Cucurbita argyrosperma subsp. ... [more]
XP_022957880.12.3e-26590.13aluminum-activated malate transporter 9-like [Cucurbita moschata][more]
XP_022996317.15.3e-25487.38aluminum-activated malate transporter 9-like [Cucurbita maxima][more]
XP_023532729.15.7e-24885.90aluminum-activated malate transporter 9-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9LPQ81.1e-6835.20Putative aluminum-activated malate transporter 3 OS=Arabidopsis thaliana OX=3702... [more]
Q9LS461.1e-6833.99Aluminum-activated malate transporter 9 OS=Arabidopsis thaliana OX=3702 GN=ALMT9... [more]
Q9C6L81.5e-6532.12Aluminum-activated malate transporter 4 OS=Arabidopsis thaliana OX=3702 GN=ALMT4... [more]
Q9SHM11.6e-6431.35Aluminum-activated malate transporter 6 OS=Arabidopsis thaliana OX=3702 GN=ALMT6... [more]
Q93Z292.6e-6232.06Aluminum-activated malate transporter 5 OS=Arabidopsis thaliana OX=3702 GN=ALMT5... [more]
Match NameE-valueIdentityDescription
A0A6J1H1V71.1e-26590.13aluminum-activated malate transporter 9-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1K1K62.6e-25487.38aluminum-activated malate transporter 9-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1CX809.6e-19370.00aluminum-activated malate transporter 9-like OS=Momordica charantia OX=3673 GN=L... [more]
A0A0A0KMG71.6e-18768.96Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G151600 PE=3 SV=1[more]
A0A4U5PLY74.8e-17664.50Uncharacterized protein OS=Populus alba OX=43335 GN=D5086_0000211980 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G18420.17.8e-7035.20Aluminium activated malate transporter family protein [more]
AT3G18440.17.8e-7033.99aluminum-activated malate transporter 9 [more]
AT1G25480.11.1e-6632.12Aluminium activated malate transporter family protein [more]
AT2G17470.11.2e-6531.35Aluminium activated malate transporter family protein [more]
AT1G68600.11.9e-6332.06Aluminium activated malate transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020966Aluminum-activated malate transporterPFAMPF11744ALMTcoord: 119..482
e-value: 2.6E-87
score: 293.7
coord: 42..118
e-value: 1.4E-31
score: 109.8
NoneNo IPR availablePANTHERPTHR31086ALUMINUM-ACTIVATED MALATE TRANSPORTER 10coord: 1..118
coord: 117..490
NoneNo IPR availablePANTHERPTHR31086:SF17OS01G0221600 PROTEINcoord: 1..118
coord: 117..490

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg02563-RACarg02563-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034220 ion transmembrane transport
biological_process GO:0015743 malate transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane