Carg02556 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg02556
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Endoglucanase
Location	Carg_Chr02: 8124792 .. 8126766 (+)
RNA-Seq Expression	Carg02556
Synteny	Carg02556

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGCTACTACTACTACCTCCATCCTTCATTTGGGCCACTTCCATCCTTCTTTGTGGCCTCCTCTCGCCCACCCTTGGCGTTGGTGGGGCTGCACATCCTCCCTTTAACTACAGCGATGCGCTGGACAAGGCCGTGCTGTTCTTTGAGGGGCAGAGGTCAGGCAAGTTACCTGTTGCTCAACGAGTCATGTGGCGCGGTGACTCTGCCCTCTCTGACGGAGCCCATGAGAATGTATCACAAAACACTACCACACCATATCAAGTTGTAAGAATATTGAGTTAAATTTTTGCAGGTGAATTTGGTGGGAGGTTACTACGATGCCGGAGACAACGTCAAGTTGGTGTGGCCGATGGCGTTTACGGTGACATTGCTGAGTTGGACCGCCGTTGAATATGAAAATGAGATGAGTTCTGTGATGCAACTACAACACCTCCGGGGATCCGTCCGGTGGGGCGCCGATTTCATATTGCAAGCTCATGTTTCACCCACCGCACTCTATACTCAGGTTTTCATATTATTTCCTATTAATATATATTTCCTTAAAAATTCTTATTTTCATTTATTGTTTATATATTTATATTTATAACGTCACCCTTTCAATATTTTCATGCATGCTTACATTTTATTTTATAATATATGTGACGGAGATTTGAACTTCTTATCTATTAATCAATGATACATGTTATATATCAAATGAGATATGTTTACATTGTCAAAAATAAAATGAGATCCCGTCGGTTGGAGATGGGAATGAAACATTTTTTATAATGGTTAGAAAACATCTCTCGTTTTAAAACTTTGAGAAGAAGCTCAAAAGAAAAAATTCAAAAATGACAATATCTACTAGCGATGAGTTTGGACGGCTATAAATGATATCAAAGCCAGACATGGGGCGATGTGTCAGCAAGGAGGCTAAGTGGGGGTGTTTGGATGACTACAAATGATATCAAAGCCAGACATATGAGAATGTACCAACAAGGAAGTTAAGCCTTGGAAGGGTGTTTGGATGGCTATAAATGATATTAAAGCTAGACACCGAGCGATGTGCCAACAAGAAGGCTGAGCCTTAAAGGGAGTGTTTGATCGGCTATAAATGATATCAAAGCTGGATACTGGGCGATGTGGGAGCAAGGCTAAGCCTTGAAGGGGGTGTACATCTCTGGTTAAGAGACACATTTTAACCATATACAGAGGAATTTCACTTTATTCTAACAACCTCAACTTTTTTACAGGAATTCTATAAAGGAAAGAGAGAATTGAGCAAGTATAAGAGCGATATAGAATCGTTCATCTGTGCAGTGATGCCCAACAGTGGCTCTTCTCAGATTCCTACAACGCCTGGTACAATCACACACCTTTCTTCCATGAAAATACATACATATATATGAGCTTTTGTGCCTCTTGCTTTGTAGGTGGCCTTCTTTTCATAAGAGATAGCACCAATTTGCAATATGCATCAACCTCTTCCATGGTGCTTTTCATGTACTCTAAACTCCTAAACCAAGCCCACATCGATGGAGTTCATTGCAGATCCAAATATTTCTCCTCTTCTCAAATCAAAATCTTCGCAAAATCCCAAGTAATTAAGTTCACTGATTCATTTGCAGCAAACCCAAGTTCATAATTTAATCATAAACTCTGTTCTTTATAGGTGGATTACATATTGGGGGAAAACCCCTTGAAGATGTCATACATGGTGGGATTCGGCAGCAAATACCCATTGAAGTTACATCACAGAGCCTCATCCATCCCTTCAATTAAAGCTCATCTGTCCAAGGTTGGGTGCAGCCATGGCTACTCGGAGTACTTCAATTCAAACAATCCAAACCCGAATATACACGTTGGTGCCATAGTGGGAGGCCCTAATTCAAACGACCAATTCAGTGATATGAGACCCGACCACACTCATTCTGAGCCTACTACTTATATGAACGCTGCGTTTGTTGGCTCAGTGGCTGCATTGGTTGCATAG

mRNA sequence

ATGCCGCTACTACTACTACCTCCATCCTTCATTTGGGCCACTTCCATCCTTCTTTGTGGCCTCCTCTCGCCCACCCTTGGCGTTGGTGGGGCTGCACATCCTCCCTTTAACTACAGCGATGCGCTGGACAAGGCCGTGCTGTTCTTTGAGGGGCAGAGGTCAGGCAAGTTACCTGTTGCTCAACGAGTCATGTGGCGCGGTGACTCTGCCCTCTCTGACGGAGCCCATGAGAATGTGAATTTGGTGGGAGGTTACTACGATGCCGGAGACAACGTCAAGTTGGTGTGGCCGATGGCGTTTACGGTGACATTGCTGAGTTGGACCGCCGTTGAATATGAAAATGAGATGAGTTCTGTGATGCAACTACAACACCTCCGGGGATCCGTCCGGTGGGGCGCCGATTTCATATTGCAAGCTCATGTTTCACCCACCGCACTCTATACTCAGGAATTCTATAAAGGAAAGAGAGAATTGAGCAAGTATAAGAGCGATATAGAATCGTTCATCTGTGCAGTGATGCCCAACAGTGGCTCTTCTCAGATTCCTACAACGCCTGGTGGCCTTCTTTTCATAAGAGATAGCACCAATTTGCAATATGCATCAACCTCTTCCATGGTGCTTTTCATGTACTCTAAACTCCTAAACCAAGCCCACATCGATGGAGTTCATTGCAGATCCAAATATTTCTCCTCTTCTCAAATCAAAATCTTCGCAAAATCCCAAGTGGATTACATATTGGGGGAAAACCCCTTGAAGATGTCATACATGGTGGGATTCGGCAGCAAATACCCATTGAAGTTACATCACAGAGCCTCATCCATCCCTTCAATTAAAGCTCATCTGTCCAAGGTTGGGTGCAGCCATGGCTACTCGGAGTACTTCAATTCAAACAATCCAAACCCGAATATACACGTTGGTGCCATAGTGGGAGGCCCTAATTCAAACGACCAATTCAGTGATATGAGACCCGACCACACTCATTCTGAGCCTACTACTTATATGAACGCTGCGTTTGTTGGCTCAGTGGCTGCATTGGTTGCATAG

Coding sequence (CDS)

ATGCCGCTACTACTACTACCTCCATCCTTCATTTGGGCCACTTCCATCCTTCTTTGTGGCCTCCTCTCGCCCACCCTTGGCGTTGGTGGGGCTGCACATCCTCCCTTTAACTACAGCGATGCGCTGGACAAGGCCGTGCTGTTCTTTGAGGGGCAGAGGTCAGGCAAGTTACCTGTTGCTCAACGAGTCATGTGGCGCGGTGACTCTGCCCTCTCTGACGGAGCCCATGAGAATGTGAATTTGGTGGGAGGTTACTACGATGCCGGAGACAACGTCAAGTTGGTGTGGCCGATGGCGTTTACGGTGACATTGCTGAGTTGGACCGCCGTTGAATATGAAAATGAGATGAGTTCTGTGATGCAACTACAACACCTCCGGGGATCCGTCCGGTGGGGCGCCGATTTCATATTGCAAGCTCATGTTTCACCCACCGCACTCTATACTCAGGAATTCTATAAAGGAAAGAGAGAATTGAGCAAGTATAAGAGCGATATAGAATCGTTCATCTGTGCAGTGATGCCCAACAGTGGCTCTTCTCAGATTCCTACAACGCCTGGTGGCCTTCTTTTCATAAGAGATAGCACCAATTTGCAATATGCATCAACCTCTTCCATGGTGCTTTTCATGTACTCTAAACTCCTAAACCAAGCCCACATCGATGGAGTTCATTGCAGATCCAAATATTTCTCCTCTTCTCAAATCAAAATCTTCGCAAAATCCCAAGTGGATTACATATTGGGGGAAAACCCCTTGAAGATGTCATACATGGTGGGATTCGGCAGCAAATACCCATTGAAGTTACATCACAGAGCCTCATCCATCCCTTCAATTAAAGCTCATCTGTCCAAGGTTGGGTGCAGCCATGGCTACTCGGAGTACTTCAATTCAAACAATCCAAACCCGAATATACACGTTGGTGCCATAGTGGGAGGCCCTAATTCAAACGACCAATTCAGTGATATGAGACCCGACCACACTCATTCTGAGCCTACTACTTATATGAACGCTGCGTTTGTTGGCTCAGTGGCTGCATTGGTTGCATAG

Protein sequence

MPLLLLPPSFIWATSILLCGLLSPTLGVGGAAHPPFNYSDALDKAVLFFEGQRSGKLPVAQRVMWRGDSALSDGAHENVNLVGGYYDAGDNVKLVWPMAFTVTLLSWTAVEYENEMSSVMQLQHLRGSVRWGADFILQAHVSPTALYTQEFYKGKRELSKYKSDIESFICAVMPNSGSSQIPTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQAHIDGVHCRSKYFSSSQIKIFAKSQVDYILGENPLKMSYMVGFGSKYPLKLHHRASSIPSIKAHLSKVGCSHGYSEYFNSNNPNPNIHVGAIVGGPNSNDQFSDMRPDHTHSEPTTYMNAAFVGSVAALVA

Homology

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BLAST of Carg02556 vs. NCBI nr
Match: KAG7036014.1 (hypothetical protein SDJN02_02814, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 705.7 bits (1820), Expect = 2.0e-199
Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 0


Query: 1   MPLLLLPPSFIWATSILLCGLLSPTLGVGGAAHPPFNYSDALDKAVLFFEGQRSGKLPVA 60
           MPLLLLPPSFIWATSILLCGLLSPTLGVGGAAHPPFNYSDALDKAVLFFEGQRSGKLPVA
Sbjct: 1   MPLLLLPPSFIWATSILLCGLLSPTLGVGGAAHPPFNYSDALDKAVLFFEGQRSGKLPVA 60

Query: 61  QRVMWRGDSALSDGAHENVNLVGGYYDAGDNVKLVWPMAFTVTLLSWTAVEYENEMSSVM 120
           QRVMWRGDSALSDGAHENVNLVGGYYDAGDNVKLVWPMAFTVTLLSWTAVEYENEMSSVM
Sbjct: 61  QRVMWRGDSALSDGAHENVNLVGGYYDAGDNVKLVWPMAFTVTLLSWTAVEYENEMSSVM 120

Query: 121 QLQHLRGSVRWGADFILQAHVSPTALYTQEFYKGKRELSKYKSDIESFICAVMPNSGSSQ 180
           QLQHLRGSVRWGADFILQAHVSPTALYTQEFYKGKRELSKYKSDIESFICAVMPNSGSSQ
Sbjct: 121 QLQHLRGSVRWGADFILQAHVSPTALYTQEFYKGKRELSKYKSDIESFICAVMPNSGSSQ 180

Query: 181 IPTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQAHIDGVHCRSKYFSSSQIKIFAKS 240
           IPTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQAHIDGVHCRSKYFSSSQIKIFAKS
Sbjct: 181 IPTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQAHIDGVHCRSKYFSSSQIKIFAKS 240

Query: 241 QVDYILGENPLKMSYMVGFGSKYPLKLHHRASSIPSIKAHLSKVGCSHGYSEYFNSNNPN 300
           QVDYILGENPLKMSYMVGFGSKYPLKLHHRASSIPSIKAHLSKVGCSHGYSEYFNSNNPN
Sbjct: 241 QVDYILGENPLKMSYMVGFGSKYPLKLHHRASSIPSIKAHLSKVGCSHGYSEYFNSNNPN 300

Query: 301 PNIHVGAIVGGPNSNDQFSDMRPDHTHSEPTTYMNAAFVGSVAALVA 348
           PNIHVGAIVGGPNSNDQFSDMRPDHTHSEPTTYMNAAFVGSVAALVA
Sbjct: 301 PNIHVGAIVGGPNSNDQFSDMRPDHTHSEPTTYMNAAFVGSVAALVA 347

[Close]

BLAST of Carg02556 vs. NCBI nr
Match: KAG6606068.1 (hypothetical protein SDJN03_03385, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 700.7 bits (1807), Expect = 6.5e-198
Identity = 343/347 (98.85%), Postives = 344/347 (99.14%), Query Frame = 0


Query: 1   MPLLLLPPSFIWATSILLCGLLSPTLGVGGAAHPPFNYSDALDKAVLFFEGQRSGKLPVA 60
           MPLLLLPPSFIWATSILLCGLLSPTLGVGGAAHPPFNYSDALDKAVLFFEGQRSGKLP+A
Sbjct: 1   MPLLLLPPSFIWATSILLCGLLSPTLGVGGAAHPPFNYSDALDKAVLFFEGQRSGKLPIA 60

Query: 61  QRVMWRGDSALSDGAHENVNLVGGYYDAGDNVKLVWPMAFTVTLLSWTAVEYENEMSSVM 120
           QRVMWRGDSALSDGAHENVNLVGGYYDAGDNVK VWPMAFTVTLLSWTAVEYENEM SVM
Sbjct: 61  QRVMWRGDSALSDGAHENVNLVGGYYDAGDNVKFVWPMAFTVTLLSWTAVEYENEMGSVM 120

Query: 121 QLQHLRGSVRWGADFILQAHVSPTALYTQEFYKGKRELSKYKSDIESFICAVMPNSGSSQ 180
           QLQHLRGSVRWGADFILQAHVSPT LYTQEFYKGKRELSKYKSDIESFICAVMPNSGSSQ
Sbjct: 121 QLQHLRGSVRWGADFILQAHVSPTTLYTQEFYKGKRELSKYKSDIESFICAVMPNSGSSQ 180

Query: 181 IPTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQAHIDGVHCRSKYFSSSQIKIFAKS 240
           IPTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQAHIDGVHCRSKYFSSSQIKIFAKS
Sbjct: 181 IPTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQAHIDGVHCRSKYFSSSQIKIFAKS 240

Query: 241 QVDYILGENPLKMSYMVGFGSKYPLKLHHRASSIPSIKAHLSKVGCSHGYSEYFNSNNPN 300
           QVDYILGENPLKMSYMVGFGSKYPLKLHHRASSIPSIKAHLSKVGCSHGYSEYFNSNNPN
Sbjct: 241 QVDYILGENPLKMSYMVGFGSKYPLKLHHRASSIPSIKAHLSKVGCSHGYSEYFNSNNPN 300

Query: 301 PNIHVGAIVGGPNSNDQFSDMRPDHTHSEPTTYMNAAFVGSVAALVA 348
           PNIHVGAIVGGPNSNDQFSDMRPDHTHSEPTTYMNAAFVGSVAALVA
Sbjct: 301 PNIHVGAIVGGPNSNDQFSDMRPDHTHSEPTTYMNAAFVGSVAALVA 347

[Close]

BLAST of Carg02556 vs. NCBI nr
Match: XP_022958537.1 (endoglucanase-like [Cucurbita moschata])

HSP 1 Score: 636.7 bits (1641), Expect = 1.1e-178
Identity = 342/491 (69.65%), Postives = 343/491 (69.86%), Query Frame = 0


Query: 1   MPLLLLPPSFIWATSILLCGLLSPTLGVGGAAHPPFNYSDALDKAVLFFEGQRSGKLPVA 60
           MPLLLLPPSFIWATSILLCGLLSPTLGVGGAAHPPFNYSDALDKAVLFFEGQRSGKLPVA
Sbjct: 1   MPLLLLPPSFIWATSILLCGLLSPTLGVGGAAHPPFNYSDALDKAVLFFEGQRSGKLPVA 60

Query: 61  QRVMWRGDSALSDGAHENVNLVGGYYDAGDNVKLVWPMAFTVTLLSWTAVEYENEMSSVM 120
           QRVMWRGDSALSDGAHENVNLVGGYYDAGDNVK VWPMAFTVTLLSWTAVEYENEM SVM
Sbjct: 61  QRVMWRGDSALSDGAHENVNLVGGYYDAGDNVKFVWPMAFTVTLLSWTAVEYENEMGSVM 120

Query: 121 QLQHLRGSVRWGADFILQAHVSPTALYTQ------------------------------- 180
           QLQHLRGSVRWGADFILQAHVSPT LYTQ                               
Sbjct: 121 QLQHLRGSVRWGADFILQAHVSPTTLYTQVGDANGDHRCWERPEDMDTPRTLYKITPTSP 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 GSEAAAEAAAALAGASIVFSHVDANYSRTLLQRSISLFQFADEFRGSYSASCPFYCSFSG 240

Query: 241 -----------------------------------------------------EFYKGKR 300
                                                                EFYKGKR
Sbjct: 241 YQDELLWAAAWLYKATGNNNYLSYVVSNQGWSQAVSEFSWDNKFVGAQTLLAKEFYKGKR 300

Query: 301 ELSKYKSDIESFICAVMPNSGSSQIPTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQ 348
           ELSK+KSDIESFICAVMPNSGSSQIPTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQ
Sbjct: 301 ELSKFKSDIESFICAVMPNSGSSQIPTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQ 360

[Close]

BLAST of Carg02556 vs. NCBI nr
Match: KAA0042719.1 (endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 473.0 bits (1216), Expect = 2.2e-129
Identity = 261/481 (54.26%), Postives = 291/481 (60.50%), Query Frame = 0


Query: 11  IWATSILLCGLLSPTLGVGGAAHPPFNYSDALDKAVLFFEGQRSGKLPVAQRVMWRGDSA 70
           IW T I LC ++  T          FNYSDAL KAVLFFEGQRSGKLP  QRV WRGDSA
Sbjct: 9   IWCTWI-LCNVVFTTSDWAADLDRTFNYSDALGKAVLFFEGQRSGKLPKTQRVKWRGDSA 68

Query: 71  LSDGAHENVNLVGGYYDAGDNVKLVWPMAFTVTLLSWTAVEYENEMSSVMQLQHLRGSVR 130
           LSDG++ENVNLVGGYYDAGDNVK  WPMAFTVTLLSWT VEYE E+ SVMQL+HLR SVR
Sbjct: 69  LSDGSYENVNLVGGYYDAGDNVKFGWPMAFTVTLLSWTTVEYEKEIKSVMQLEHLRSSVR 128

Query: 131 WGADFILQAHVSPTALYTQ----------------------------------------- 190
           WGADFIL+AHVSPT LYTQ                                         
Sbjct: 129 WGADFILRAHVSPTTLYTQVGDANGDHQCWERPEDMDTPRTLYKITPDSPGTEAAAEAAA 188

Query: 191 ------------------------------------------------------------ 250
                                                                       
Sbjct: 189 ALAAASIVFNHVNTNYSRKLLQHSKSLFEFADKFRGSYSASCPFYCSYSGYQDELLWAAA 248

Query: 251 -------------------------------------------EFYKGKRELSKYKSDIE 310
                                                      EFYKGK+ LSK+K+D+E
Sbjct: 249 WLYKASRNMKYLRYVLSNQWWSQPTSEFSWDNKFVGAQTLLTKEFYKGKKNLSKFKNDVE 308

Query: 311 SFICAVMPNSGSSQIPTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQAHIDGVHCRS 348
           SFIC VMP+ GSS+I  T GGLLF+RDS+NLQYAS+SSMVLFMYS+LL QAHI G+HC S
Sbjct: 309 SFICKVMPDGGSSEILRTNGGLLFLRDSSNLQYASSSSMVLFMYSRLLKQAHIHGIHCGS 368

[Close]

BLAST of Carg02556 vs. NCBI nr
Match: XP_008437724.1 (PREDICTED: endoglucanase-like [Cucumis melo])

HSP 1 Score: 470.7 bits (1210), Expect = 1.1e-128
Identity = 261/481 (54.26%), Postives = 290/481 (60.29%), Query Frame = 0


Query: 11  IWATSILLCGLLSPTLGVGGAAHPPFNYSDALDKAVLFFEGQRSGKLPVAQRVMWRGDSA 70
           IW T I LC ++  T          FNYSDAL KAVLFFEGQRSGKLP  QRV WRGDSA
Sbjct: 9   IWCTWI-LCNVVFTTSDWAADLDRTFNYSDALGKAVLFFEGQRSGKLPKTQRVKWRGDSA 68

Query: 71  LSDGAHENVNLVGGYYDAGDNVKLVWPMAFTVTLLSWTAVEYENEMSSVMQLQHLRGSVR 130
           LSDG++ENVNLVGGYYDAGDNVK  WPMAFTVTLLSWT VEYE E+ SVMQL+HLR SVR
Sbjct: 69  LSDGSYENVNLVGGYYDAGDNVKFGWPMAFTVTLLSWTTVEYEKEIKSVMQLEHLRSSVR 128

Query: 131 WGADFILQAHVSPTALYTQ----------------------------------------- 190
           WGADFIL+AHVSPT LYTQ                                         
Sbjct: 129 WGADFILRAHVSPTTLYTQVGDANGDHQCWERPEDMDTPRTLYKITPDSPGTEAAAEAAA 188

Query: 191 ------------------------------------------------------------ 250
                                                                       
Sbjct: 189 ALAAASIVFNHVNTNYSRKLLQHSKSLFEFADKFRGSYSASCPFYCSYSGYQDELLWAAA 248

Query: 251 -------------------------------------------EFYKGKRELSKYKSDIE 310
                                                      EFYKGK+ LSK+K+D+E
Sbjct: 249 WLYKASRNMKYLGYVLSNQWWSQPTSEFSWDNKFVGAQTLLTKEFYKGKKNLSKFKNDVE 308

Query: 311 SFICAVMPNSGSSQIPTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQAHIDGVHCRS 348
           SFIC VMP+ GSS+I  T GGLLF+RDS+NLQYAS+SSMVLFMYSKLL QA I G+HC S
Sbjct: 309 SFICKVMPDGGSSEILRTNGGLLFLRDSSNLQYASSSSMVLFMYSKLLKQADIHGIHCGS 368

[Close]

BLAST of Carg02556 vs. ExPASy Swiss-Prot
Match: P22503 (Endoglucanase OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 412.9 bits (1060), Expect = 3.5e-114
Identity = 223/484 (46.07%), Postives = 284/484 (58.68%), Query Frame = 0


Query: 10  FIWATSILLCGLLSPTLG--VGGAAHPPFNYSDALDKAVLFFEGQRSGKLPVAQRVMWRG 69
           F+W++ +   GL     G     +  P ++Y+DAL KA+LFFEGQRSGKLP +QRV WR 
Sbjct: 11  FLWSSMVCHNGLAMMDDGKLTSSSGPPNYDYADALAKAILFFEGQRSGKLPSSQRVKWRE 70

Query: 70  DSALSDGAHENVNLVGGYYDAGDNVKLVWPMAFTVTLLSWTAVEYENEMSSVMQLQHLRG 129
           DSALSDG  +NVNL+GGYYDAGDNVK  WPMAF+ +LLSW AVEYE+E+SSV QL +L+ 
Sbjct: 71  DSALSDGKLQNVNLMGGYYDAGDNVKFGWPMAFSTSLLSWAAVEYESEISSVNQLGYLQS 130

Query: 130 SVRWGADFILQAHVSPTALYTQ-------------------------------------- 189
           ++RWGADF+L+AH SPT LYTQ                                      
Sbjct: 131 AIRWGADFMLRAHTSPTTLYTQVGDGNADHNCWERPEDMDTPRTVYKIDANSPGTEVAAE 190

Query: 190 ------------------------------------------------------------ 249
                                                                       
Sbjct: 191 YAAALSAASIVFKKIDAKYSSTLLSHSKSLFDFADKNRGSYSGSCPFYCSYSGYQDELLW 250

Query: 250 ----------------------------------------------EFYKGKRELSKYKS 309
                                                         EFY GK++L+K K+
Sbjct: 251 AAAWLYKASGESKYLSYIISNQGWSQTVSEFSWDNKFVGAQTLLTEEFYGGKKDLAKIKT 310

Query: 310 DIESFICAVMPNSGSSQIPTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQAHIDGVH 348
           D ESFICAVMP S S QI TTPGGLLF RDS+NLQY ++S+MVLF++S++LN+ HI+G++
Sbjct: 311 DAESFICAVMPGSNSRQIKTTPGGLLFTRDSSNLQYTTSSTMVLFIFSRILNRNHINGIN 370

[Close]

BLAST of Carg02556 vs. ExPASy Swiss-Prot
Match: Q9SUS0 (Endoglucanase 20 OS=Arabidopsis thaliana OX=3702 GN=At4g23560 PE=2 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 2.7e-106
Identity = 206/454 (45.37%), Postives = 257/454 (56.61%), Query Frame = 0


Query: 38  YSDALDKAVLFFEGQRSGKLPVAQRVMWRGDSALSDGAHENVNLVGGYYDAGDNVKLVWP 97
           Y DAL+K++LFFEGQRSGKLP  QRV WR DSALSDG+  NVNL+GGYYDAGDNVK VWP
Sbjct: 24  YGDALNKSILFFEGQRSGKLPTNQRVKWRADSALSDGSLANVNLIGGYYDAGDNVKFVWP 83

Query: 98  MAFTVTLLSWTAVEYENEMSSVMQLQHLRGSVRWGADFILQAHVSPTALYTQ-------- 157
           M+FT TLLSW A+EY+NE+SSV QL +LR +++WG DFIL+AH SP  LYTQ        
Sbjct: 84  MSFTTTLLSWAAIEYQNEISSVNQLGYLRSTIKWGTDFILRAHTSPNMLYTQVGDGNSDH 143

Query: 158 ------------------------------------------------------------ 217
                                                                       
Sbjct: 144 SCWERPEDMDTSRTLYSISSSSPGSEAAGEAAAALAAASLVFKSVDSTYSSTLLNHAKTL 203

Query: 218 ------------------------------------------------------------ 277
                                                                       
Sbjct: 204 FEFADKYRGSYQASCPFYCSYSGYQDELLWAAAWLYKATGDKIYINYVISNKDWSQAVNE 263

Query: 278 ----------------EFYKGKRELSKYKSDIESFICAVMPNSGSSQIPTTPGGLLFIRD 337
                           EFY G  +L+K+KSD+ESF+CA+MP S S QI  TPGGLLFIRD
Sbjct: 264 FSWDNKFVGAQALLVSEFYNGANDLAKFKSDVESFVCAMMPGSSSQQIKPTPGGLLFIRD 323

Query: 338 STNLQYASTSSMVLFMYSKLLNQAHIDGVHCRSKYFSSSQIKIFAKSQVDYILGENPLKM 348
           S+NLQY +T++ VLF YSK L +A +  + C S  F+ SQI+ FAKSQVDYILG NP+KM
Sbjct: 324 SSNLQYVTTATTVLFHYSKTLTKAGVGSIQCGSTKFTVSQIRNFAKSQVDYILGNNPMKM 383

[Close]

BLAST of Carg02556 vs. ExPASy Swiss-Prot
Match: Q9SZ90 (Endoglucanase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g09740 PE=3 SV=2)

HSP 1 Score: 375.6 bits (963), Expect = 6.3e-103
Identity = 200/455 (43.96%), Postives = 253/455 (55.60%), Query Frame = 0


Query: 37  NYSDALDKAVLFFEGQRSGKLPVAQRVMWRGDSALSDGAHENVNLVGGYYDAGDNVKLVW 96
           +Y DAL+K++LFFEGQRSGKLP  QRV WR DS LSDGA  NVNL+GGYYDAGDNVK VW
Sbjct: 23  DYGDALNKSILFFEGQRSGKLPTNQRVKWRADSGLSDGASANVNLIGGYYDAGDNVKFVW 82

Query: 97  PMAFTVTLLSWTAVEYENEMSSVMQLQHLRGSVRWGADFILQAHVSPTALYTQ------- 156
           PM+FT TLLSW A+EY+NE++ V QL +LR +++WG +FIL+AH S   LYTQ       
Sbjct: 83  PMSFTTTLLSWAALEYQNEITFVNQLGYLRSTIKWGTNFILRAHTSTNMLYTQVGDGNSD 142

Query: 157 ------------------------------------------------------------ 216
                                                                       
Sbjct: 143 HSCWERPEDMDTPRTLYSISSSSPGSEAAGEAAAALAAASLVFKLVDSTYSSKLLNNAKS 202

Query: 217 ------------------------------------------------------------ 276
                                                                       
Sbjct: 203 LFEFADKYRGSYQASCPFYCSHSGYQDELLWAAAWLYKATGEKSYLNYVISNKDWSKAIN 262

Query: 277 -----------------EFYKGKRELSKYKSDIESFICAVMPNSGSSQIPTTPGGLLFIR 336
                            EFY G  +L K+K+D+ESF+CA+MP S S QI  TPGG+LFIR
Sbjct: 263 EFSWDNKFAGVQALLASEFYNGANDLEKFKTDVESFVCALMPGSSSQQIKPTPGGILFIR 322

Query: 337 DSTNLQYASTSSMVLFMYSKLLNQAHIDGVHCRSKYFSSSQIKIFAKSQVDYILGENPLK 348
           DS+NLQY +T++ +LF YSK L +A +  + C S  F+ SQI+ FAKSQVDYILG NPLK
Sbjct: 323 DSSNLQYVTTATTILFYYSKTLTKAGVGSIQCGSTQFTVSQIRNFAKSQVDYILGNNPLK 382

[Close]

BLAST of Carg02556 vs. ExPASy Swiss-Prot
Match: Q6ZA06 (Endoglucanase 20 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU15 PE=2 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 1.1e-96
Identity = 198/463 (42.76%), Postives = 248/463 (53.56%), Query Frame = 0


Query: 27  GVGGAAHPPFNYSDALDKAVLFFEGQRSGKLPVAQRVMWRGDSALSDGAHENVNLVGGYY 86
           G+G    P  +Y DAL KA+LFFEGQRSG+LP  QR  WRGDSAL+DG  ENVNL GGYY
Sbjct: 32  GLGRLGSP--DYGDALAKAILFFEGQRSGRLPANQRATWRGDSALTDGREENVNLTGGYY 91

Query: 87  DAGDNVKLVWPMAFTVTLLSWTAVEYENEMSSVMQLQHLRGSVRWGADFILQAHVSPTAL 146
           DAGDNVK  +PMAFTVTLL W+AVEY   +++  +L +LR ++RWGADF+L+AH SPT L
Sbjct: 92  DAGDNVKFGYPMAFTVTLLGWSAVEYGAAVAAAGELGNLRAAIRWGADFLLRAHASPTTL 151

Query: 147 YT---------------------------------------------------------- 206
           YT                                                          
Sbjct: 152 YTQVGDGNADHQCWERPEDMDTPRTLYKITADSPGSEAAAEASAALAAAYVALKDDGDTA 211

Query: 207 ------------------------------------------------------------ 266
                                                                       
Sbjct: 212 FSSRLLAASRSLFDFANNYRGSFQSSCPFYCSYSGFQDELLWASAWLFKATRDAKYLDFL 271

Query: 267 ---------------------------QEFYKGKRELSKYKSDIESFICAVMPNSGSSQI 326
                                      QE+  G+ +L++YK +++SF+CA+MPNSG+ QI
Sbjct: 272 TNNQGSSNPVNEFSWDNKYAGAQMLAAQEYLGGRTQLARYKDNLDSFVCALMPNSGNVQI 331

Query: 327 PTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQAHIDGVHCRSKYFSSSQIKIFAKSQ 345
            TTPGGLLF RDS NLQY +T+++VL +YSK+L  +   GV C +  FS +QI  FA SQ
Sbjct: 332 RTTPGGLLFTRDSVNLQYTTTATLVLSIYSKVLKSSGSRGVRCSAATFSPNQISSFATSQ 391

[Close]

BLAST of Carg02556 vs. ExPASy Swiss-Prot
Match: Q8LQ92 (Endoglucanase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU8 PE=2 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 3.1e-70
Identity = 160/464 (34.48%), Postives = 223/464 (48.06%), Query Frame = 0


Query: 35  PFNYSDALDKAVLFFEGQRSGKLPVAQRVMWRGDSALSDGAHENVNLVGGYYDAGDNVKL 94
           P +Y DAL K++LFFEGQRSGKLP +QRV WRGDS LSDG+   V+LVGGYYDAGDN+K 
Sbjct: 33  PHDYRDALTKSILFFEGQRSGKLPPSQRVSWRGDSGLSDGSSIKVDLVGGYYDAGDNMKF 92

Query: 95  VWPMAFTVTLLSWTAVEYENEMSSVMQLQHLRGSVRWGADFILQAHVSPTALYTQ----- 154
            +P+AF++T+L+W+ VE+   M    +LQH R +VRWG+D++L+A   P  +Y Q     
Sbjct: 93  GFPLAFSMTMLAWSVVEFGGLMKG--ELQHARDAVRWGSDYLLKATAHPDTVYVQVGDAN 152

Query: 155 ------------------------------------------------------------ 214
                                                                       
Sbjct: 153 RDHACWERPEDMDTPRTVYKVDPSTPGTDVAAETAAALAAASLVFRKSDPAYASRLVARA 212

Query: 215 ----EF----------------------YKGKRE-------------------------- 274
               EF                      Y G ++                          
Sbjct: 213 KRVFEFADKHRGTYSTRLSPYVCPYYCSYSGYQDELLWGAAWLHRATKNPTYLSYIQMNG 272

Query: 275 ------------------------------------LSKYKSDIESFICAVMPNSGSSQI 334
                                               L +YK   +SFIC+++P + + Q 
Sbjct: 273 QVLGADEQDNTFGWDNKHAGARILIAKAFLVQKVAALHEYKGHADSFICSMVPGTPTDQT 332

Query: 335 PTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQAHIDGVHCRSKYFSSSQIKIFAKSQ 346
             T GGLLF    +N+QY ++SS +L  Y+K L  +    V C     + ++++  A+ Q
Sbjct: 333 QYTRGGLLFKLSDSNMQYVTSSSFLLLTYAKYLAFSKTT-VSCGGAAVTPARLRAIARQQ 392

[Close]

BLAST of Carg02556 vs. ExPASy TrEMBL
Match: A0A6J1H239 (Endoglucanase OS=Cucurbita moschata OX=3662 GN=LOC111459740 PE=3 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 5.6e-179
Identity = 342/491 (69.65%), Postives = 343/491 (69.86%), Query Frame = 0


Query: 1   MPLLLLPPSFIWATSILLCGLLSPTLGVGGAAHPPFNYSDALDKAVLFFEGQRSGKLPVA 60
           MPLLLLPPSFIWATSILLCGLLSPTLGVGGAAHPPFNYSDALDKAVLFFEGQRSGKLPVA
Sbjct: 1   MPLLLLPPSFIWATSILLCGLLSPTLGVGGAAHPPFNYSDALDKAVLFFEGQRSGKLPVA 60

Query: 61  QRVMWRGDSALSDGAHENVNLVGGYYDAGDNVKLVWPMAFTVTLLSWTAVEYENEMSSVM 120
           QRVMWRGDSALSDGAHENVNLVGGYYDAGDNVK VWPMAFTVTLLSWTAVEYENEM SVM
Sbjct: 61  QRVMWRGDSALSDGAHENVNLVGGYYDAGDNVKFVWPMAFTVTLLSWTAVEYENEMGSVM 120

Query: 121 QLQHLRGSVRWGADFILQAHVSPTALYTQ------------------------------- 180
           QLQHLRGSVRWGADFILQAHVSPT LYTQ                               
Sbjct: 121 QLQHLRGSVRWGADFILQAHVSPTTLYTQVGDANGDHRCWERPEDMDTPRTLYKITPTSP 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 GSEAAAEAAAALAGASIVFSHVDANYSRTLLQRSISLFQFADEFRGSYSASCPFYCSFSG 240

Query: 241 -----------------------------------------------------EFYKGKR 300
                                                                EFYKGKR
Sbjct: 241 YQDELLWAAAWLYKATGNNNYLSYVVSNQGWSQAVSEFSWDNKFVGAQTLLAKEFYKGKR 300

Query: 301 ELSKYKSDIESFICAVMPNSGSSQIPTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQ 348
           ELSK+KSDIESFICAVMPNSGSSQIPTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQ
Sbjct: 301 ELSKFKSDIESFICAVMPNSGSSQIPTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQ 360

[Close]

BLAST of Carg02556 vs. ExPASy TrEMBL
Match: A0A5A7TMY1 (Endoglucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002170 PE=3 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 1.1e-129
Identity = 261/481 (54.26%), Postives = 291/481 (60.50%), Query Frame = 0


Query: 11  IWATSILLCGLLSPTLGVGGAAHPPFNYSDALDKAVLFFEGQRSGKLPVAQRVMWRGDSA 70
           IW T I LC ++  T          FNYSDAL KAVLFFEGQRSGKLP  QRV WRGDSA
Sbjct: 9   IWCTWI-LCNVVFTTSDWAADLDRTFNYSDALGKAVLFFEGQRSGKLPKTQRVKWRGDSA 68

Query: 71  LSDGAHENVNLVGGYYDAGDNVKLVWPMAFTVTLLSWTAVEYENEMSSVMQLQHLRGSVR 130
           LSDG++ENVNLVGGYYDAGDNVK  WPMAFTVTLLSWT VEYE E+ SVMQL+HLR SVR
Sbjct: 69  LSDGSYENVNLVGGYYDAGDNVKFGWPMAFTVTLLSWTTVEYEKEIKSVMQLEHLRSSVR 128

Query: 131 WGADFILQAHVSPTALYTQ----------------------------------------- 190
           WGADFIL+AHVSPT LYTQ                                         
Sbjct: 129 WGADFILRAHVSPTTLYTQVGDANGDHQCWERPEDMDTPRTLYKITPDSPGTEAAAEAAA 188

Query: 191 ------------------------------------------------------------ 250
                                                                       
Sbjct: 189 ALAAASIVFNHVNTNYSRKLLQHSKSLFEFADKFRGSYSASCPFYCSYSGYQDELLWAAA 248

Query: 251 -------------------------------------------EFYKGKRELSKYKSDIE 310
                                                      EFYKGK+ LSK+K+D+E
Sbjct: 249 WLYKASRNMKYLRYVLSNQWWSQPTSEFSWDNKFVGAQTLLTKEFYKGKKNLSKFKNDVE 308

Query: 311 SFICAVMPNSGSSQIPTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQAHIDGVHCRS 348
           SFIC VMP+ GSS+I  T GGLLF+RDS+NLQYAS+SSMVLFMYS+LL QAHI G+HC S
Sbjct: 309 SFICKVMPDGGSSEILRTNGGLLFLRDSSNLQYASSSSMVLFMYSRLLKQAHIHGIHCGS 368

[Close]

BLAST of Carg02556 vs. ExPASy TrEMBL
Match: A0A1S3AVA7 (Endoglucanase OS=Cucumis melo OX=3656 GN=LOC103483071 PE=3 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 5.3e-129
Identity = 261/481 (54.26%), Postives = 290/481 (60.29%), Query Frame = 0


Query: 11  IWATSILLCGLLSPTLGVGGAAHPPFNYSDALDKAVLFFEGQRSGKLPVAQRVMWRGDSA 70
           IW T I LC ++  T          FNYSDAL KAVLFFEGQRSGKLP  QRV WRGDSA
Sbjct: 9   IWCTWI-LCNVVFTTSDWAADLDRTFNYSDALGKAVLFFEGQRSGKLPKTQRVKWRGDSA 68

Query: 71  LSDGAHENVNLVGGYYDAGDNVKLVWPMAFTVTLLSWTAVEYENEMSSVMQLQHLRGSVR 130
           LSDG++ENVNLVGGYYDAGDNVK  WPMAFTVTLLSWT VEYE E+ SVMQL+HLR SVR
Sbjct: 69  LSDGSYENVNLVGGYYDAGDNVKFGWPMAFTVTLLSWTTVEYEKEIKSVMQLEHLRSSVR 128

Query: 131 WGADFILQAHVSPTALYTQ----------------------------------------- 190
           WGADFIL+AHVSPT LYTQ                                         
Sbjct: 129 WGADFILRAHVSPTTLYTQVGDANGDHQCWERPEDMDTPRTLYKITPDSPGTEAAAEAAA 188

Query: 191 ------------------------------------------------------------ 250
                                                                       
Sbjct: 189 ALAAASIVFNHVNTNYSRKLLQHSKSLFEFADKFRGSYSASCPFYCSYSGYQDELLWAAA 248

Query: 251 -------------------------------------------EFYKGKRELSKYKSDIE 310
                                                      EFYKGK+ LSK+K+D+E
Sbjct: 249 WLYKASRNMKYLGYVLSNQWWSQPTSEFSWDNKFVGAQTLLTKEFYKGKKNLSKFKNDVE 308

Query: 311 SFICAVMPNSGSSQIPTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQAHIDGVHCRS 348
           SFIC VMP+ GSS+I  T GGLLF+RDS+NLQYAS+SSMVLFMYSKLL QA I G+HC S
Sbjct: 309 SFICKVMPDGGSSEILRTNGGLLFLRDSSNLQYASSSSMVLFMYSKLLKQADIHGIHCGS 368

[Close]

BLAST of Carg02556 vs. ExPASy TrEMBL
Match: A0A6J1DTR0 (Endoglucanase OS=Momordica charantia OX=3673 GN=LOC111023924 PE=3 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 1.2e-128
Identity = 261/483 (54.04%), Postives = 289/483 (59.83%), Query Frame = 0


Query: 9   SFIWATSILLCGLLSPTLGVGGAAHPPFNYSDALDKAVLFFEGQRSGKLPVAQRVMWRGD 68
           S IW T I+   +   + GV   A   FNY DAL KAVLFFEGQRSGKL  AQRV WRGD
Sbjct: 7   SAIWDTWIICSAVFVASRGV---AEHAFNYGDALGKAVLFFEGQRSGKLSEAQRVKWRGD 66

Query: 69  SALSDGAHENVNLVGGYYDAGDNVKLVWPMAFTVTLLSWTAVEYENEMSSVMQLQHLRGS 128
           SALSDG  ENVNLVGGYYDAGDNVK  WPMAFTV LLSWTA EYE E++SV QL HLR S
Sbjct: 67  SALSDGGLENVNLVGGYYDAGDNVKFGWPMAFTVALLSWTAAEYEEEIASVKQLDHLRSS 126

Query: 129 VRWGADFILQAHVSPTALYTQ--------------------------------------- 188
           VRWGADFIL+AH SPT LYTQ                                       
Sbjct: 127 VRWGADFILRAHTSPTTLYTQVGDGNSDHQCWERPEDMDTPRTLYKITPNSPGTEAAADA 186

Query: 189 ------------------------------------------------------------ 248
                                                                       
Sbjct: 187 AAALSAASIVFNRVDANYSRTLLQHSKSLFQFADRFRGSYSASCPFYCSYSGYQDELLWA 246

Query: 249 ---------------------------------------------EFYKGKRELSKYKSD 308
                                                        EFY+GK+ELSK+K+D
Sbjct: 247 AAWLYKASGSSKYLNYVSSNQGWSQAASEFSWDNKFAGAQTLLAKEFYQGKKELSKFKND 306

Query: 309 IESFICAVMPNSGSSQIPTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQAHIDGVHC 348
            ESFIC VMP S SS+I TTPGGLLF+RDS+NLQY  +SSMVLF+YS+LL++A + GVHC
Sbjct: 307 AESFICTVMPGSSSSRISTTPGGLLFVRDSSNLQYVCSSSMVLFIYSRLLDKAGVGGVHC 366

[Close]

BLAST of Carg02556 vs. ExPASy TrEMBL
Match: A0A6J1DX17 (Endoglucanase OS=Momordica charantia OX=3673 GN=LOC111023924 PE=3 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 8.4e-127
Identity = 261/488 (53.48%), Postives = 289/488 (59.22%), Query Frame = 0


Query: 9   SFIWATSILLCGLLSPTLGVGGAAHPPFNYSDALDKAVLFFEGQRSGKLPVAQRVMWRGD 68
           S IW T I+   +   + GV   A   FNY DAL KAVLFFEGQRSGKL  AQRV WRGD
Sbjct: 7   SAIWDTWIICSAVFVASRGV---AEHAFNYGDALGKAVLFFEGQRSGKLSEAQRVKWRGD 66

Query: 69  SALSDGAHEN-----VNLVGGYYDAGDNVKLVWPMAFTVTLLSWTAVEYENEMSSVMQLQ 128
           SALSDG  EN     VNLVGGYYDAGDNVK  WPMAFTV LLSWTA EYE E++SV QL 
Sbjct: 67  SALSDGGLENVGEKTVNLVGGYYDAGDNVKFGWPMAFTVALLSWTAAEYEEEIASVKQLD 126

Query: 129 HLRGSVRWGADFILQAHVSPTALYTQ---------------------------------- 188
           HLR SVRWGADFIL+AH SPT LYTQ                                  
Sbjct: 127 HLRSSVRWGADFILRAHTSPTTLYTQVGDGNSDHQCWERPEDMDTPRTLYKITPNSPGTE 186

Query: 189 ------------------------------------------------------------ 248
                                                                       
Sbjct: 187 AAADAAAALSAASIVFNRVDANYSRTLLQHSKSLFQFADRFRGSYSASCPFYCSYSGYQD 246

Query: 249 --------------------------------------------------EFYKGKRELS 308
                                                             EFY+GK+ELS
Sbjct: 247 ELLWAAAWLYKASGSSKYLNYVSSNQGWSQAASEFSWDNKFAGAQTLLAKEFYQGKKELS 306

Query: 309 KYKSDIESFICAVMPNSGSSQIPTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQAHI 348
           K+K+D ESFIC VMP S SS+I TTPGGLLF+RDS+NLQY  +SSMVLF+YS+LL++A +
Sbjct: 307 KFKNDAESFICTVMPGSSSSRISTTPGGLLFVRDSSNLQYVCSSSMVLFIYSRLLDKAGV 366

[Close]

BLAST of Carg02556 vs. TAIR 10
Match: AT4G23560.1 (glycosyl hydrolase 9B15 )

HSP 1 Score: 386.7 bits (992), Expect = 1.9e-107
Identity = 206/454 (45.37%), Postives = 257/454 (56.61%), Query Frame = 0


Query: 38  YSDALDKAVLFFEGQRSGKLPVAQRVMWRGDSALSDGAHENVNLVGGYYDAGDNVKLVWP 97
           Y DAL+K++LFFEGQRSGKLP  QRV WR DSALSDG+  NVNL+GGYYDAGDNVK VWP
Sbjct: 24  YGDALNKSILFFEGQRSGKLPTNQRVKWRADSALSDGSLANVNLIGGYYDAGDNVKFVWP 83

Query: 98  MAFTVTLLSWTAVEYENEMSSVMQLQHLRGSVRWGADFILQAHVSPTALYTQ-------- 157
           M+FT TLLSW A+EY+NE+SSV QL +LR +++WG DFIL+AH SP  LYTQ        
Sbjct: 84  MSFTTTLLSWAAIEYQNEISSVNQLGYLRSTIKWGTDFILRAHTSPNMLYTQVGDGNSDH 143

Query: 158 ------------------------------------------------------------ 217
                                                                       
Sbjct: 144 SCWERPEDMDTSRTLYSISSSSPGSEAAGEAAAALAAASLVFKSVDSTYSSTLLNHAKTL 203

Query: 218 ------------------------------------------------------------ 277
                                                                       
Sbjct: 204 FEFADKYRGSYQASCPFYCSYSGYQDELLWAAAWLYKATGDKIYINYVISNKDWSQAVNE 263

Query: 278 ----------------EFYKGKRELSKYKSDIESFICAVMPNSGSSQIPTTPGGLLFIRD 337
                           EFY G  +L+K+KSD+ESF+CA+MP S S QI  TPGGLLFIRD
Sbjct: 264 FSWDNKFVGAQALLVSEFYNGANDLAKFKSDVESFVCAMMPGSSSQQIKPTPGGLLFIRD 323

Query: 338 STNLQYASTSSMVLFMYSKLLNQAHIDGVHCRSKYFSSSQIKIFAKSQVDYILGENPLKM 348
           S+NLQY +T++ VLF YSK L +A +  + C S  F+ SQI+ FAKSQVDYILG NP+KM
Sbjct: 324 SSNLQYVTTATTVLFHYSKTLTKAGVGSIQCGSTKFTVSQIRNFAKSQVDYILGNNPMKM 383

[Close]

BLAST of Carg02556 vs. TAIR 10
Match: AT4G09740.1 (glycosyl hydrolase 9B14 )

HSP 1 Score: 375.6 bits (963), Expect = 4.4e-104
Identity = 200/455 (43.96%), Postives = 253/455 (55.60%), Query Frame = 0


Query: 37  NYSDALDKAVLFFEGQRSGKLPVAQRVMWRGDSALSDGAHENVNLVGGYYDAGDNVKLVW 96
           +Y DAL+K++LFFEGQRSGKLP  QRV WR DS LSDGA  NVNL+GGYYDAGDNVK VW
Sbjct: 23  DYGDALNKSILFFEGQRSGKLPTNQRVKWRADSGLSDGASANVNLIGGYYDAGDNVKFVW 82

Query: 97  PMAFTVTLLSWTAVEYENEMSSVMQLQHLRGSVRWGADFILQAHVSPTALYTQ------- 156
           PM+FT TLLSW A+EY+NE++ V QL +LR +++WG +FIL+AH S   LYTQ       
Sbjct: 83  PMSFTTTLLSWAALEYQNEITFVNQLGYLRSTIKWGTNFILRAHTSTNMLYTQVGDGNSD 142

Query: 157 ------------------------------------------------------------ 216
                                                                       
Sbjct: 143 HSCWERPEDMDTPRTLYSISSSSPGSEAAGEAAAALAAASLVFKLVDSTYSSKLLNNAKS 202

Query: 217 ------------------------------------------------------------ 276
                                                                       
Sbjct: 203 LFEFADKYRGSYQASCPFYCSHSGYQDELLWAAAWLYKATGEKSYLNYVISNKDWSKAIN 262

Query: 277 -----------------EFYKGKRELSKYKSDIESFICAVMPNSGSSQIPTTPGGLLFIR 336
                            EFY G  +L K+K+D+ESF+CA+MP S S QI  TPGG+LFIR
Sbjct: 263 EFSWDNKFAGVQALLASEFYNGANDLEKFKTDVESFVCALMPGSSSQQIKPTPGGILFIR 322

Query: 337 DSTNLQYASTSSMVLFMYSKLLNQAHIDGVHCRSKYFSSSQIKIFAKSQVDYILGENPLK 348
           DS+NLQY +T++ +LF YSK L +A +  + C S  F+ SQI+ FAKSQVDYILG NPLK
Sbjct: 323 DSSNLQYVTTATTILFYYSKTLTKAGVGSIQCGSTQFTVSQIRNFAKSQVDYILGNNPLK 382

[Close]

BLAST of Carg02556 vs. TAIR 10
Match: AT1G02800.1 (cellulase 2 )

HSP 1 Score: 259.2 bits (661), Expect = 4.6e-69
Identity = 158/462 (34.20%), Postives = 216/462 (46.75%), Query Frame = 0


Query: 37  NYSDALDKAVLFFEGQRSGKLPVAQRVMWRGDSALSDGAHENVNLVGGYYDAGDNVKLVW 96
           NY DAL K++LFFEGQRSGKLP  QR+ WR +S LSDG+  NV+LVGGYYDAGDN+K  +
Sbjct: 43  NYKDALSKSILFFEGQRSGKLPPNQRMTWRSNSGLSDGSALNVDLVGGYYDAGDNMKFGF 102

Query: 97  PMAFTVTLLSWTAVEYENEMSSVMQLQHLRGSVRWGADFILQAHVSPTALYTQ------- 156
           PMAFT T+LSW+ +E+   M S  +L + + ++RW  DF+L+A   P  +Y Q       
Sbjct: 103 PMAFTTTMLSWSLIEFGGLMKS--ELPNAKDAIRWATDFLLKATSHPDTIYVQVGDPNMD 162

Query: 157 ------------------------------------------------------------ 216
                                                                       
Sbjct: 163 HACWERPEDMDTPRSVFKVDKNNPGSDIAGEIAAALAAASIVFRKCDPSYSNHLLQRAIT 222

Query: 217 -----EFYKGK------------------------------------------------- 276
                + Y+G                                                  
Sbjct: 223 VFTFADKYRGPYSAGLAPEVCPFYCSYSGYQDELLWGAAWLQKATNNPTYLNYIKANGQI 282

Query: 277 --------------------------------RELSKYKSDIESFICAVMPNSGSSQIPT 336
                                           + L +YK   +SFIC+V+P + SSQ   
Sbjct: 283 LGADEFDNMFSWDNKHVGARILLSKEFLIQKVKSLEEYKEHADSFICSVLPGASSSQY-- 342

Query: 337 TPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQAHIDGVHCRSKYFSSSQIKIFAKSQVD 346
           TPGGLLF    +N+QY +++S +L  Y+K L  A     +C     + ++++  AK QVD
Sbjct: 343 TPGGLLFKMGESNMQYVTSTSFLLLTYAKYLTSAR-TVAYCGGSVVTPARLRSIAKKQVD 402

[Close]

BLAST of Carg02556 vs. TAIR 10
Match: AT4G02290.1 (glycosyl hydrolase 9B13 )

HSP 1 Score: 253.8 bits (647), Expect = 1.9e-67
Identity = 150/460 (32.61%), Postives = 215/460 (46.74%), Query Frame = 0


Query: 37  NYSDALDKAVLFFEGQRSGKLPVAQRVMWRGDSALSDGAHENVNLVGGYYDAGDNVKLVW 96
           NY DAL K++LFFEGQRSGKLP  QR+ WR DS LSDG+  +V+LVGGYYDAGDN+K  +
Sbjct: 52  NYKDALTKSILFFEGQRSGKLPSNQRMSWRRDSGLSDGSALHVDLVGGYYDAGDNIKFGF 111

Query: 97  PMAFTVTLLSWTAVEYENEMSSVMQLQHLRGSVRWGADFILQAHVSPTALYTQ------- 156
           PMAFT T+LSW+ +E+   M S  +LQ+ + ++RW  D++L+A   P  +Y Q       
Sbjct: 112 PMAFTTTMLSWSVIEFGGLMKS--ELQNAKIAIRWATDYLLKATSQPDTIYVQVGDANKD 171

Query: 157 ------------------------------------------------------------ 216
                                                                       
Sbjct: 172 HSCWERPEDMDTVRSVFKVDKNIPGSDVAAETAAALAAAAIVFRKSDPSYSKVLLKRAIS 231

Query: 217 -----EFYKGK------------------------------------------------- 276
                + Y+G                                                  
Sbjct: 232 VFAFADKYRGTYSAGLKPDVCPFYCSYSGYQDELLWGAAWLQKATKNIKYLNYIKINGQI 291

Query: 277 --------------------------------RELSKYKSDIESFICAVMPNSGSSQIPT 336
                                           + L +YK   ++FIC+V+P +  S    
Sbjct: 292 LGAAEYDNTFGWDNKHAGARILLTKAFLVQNVKTLHEYKGHADNFICSVIPGAPFSSTQY 351

Query: 337 TPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQAHIDGVHCRSKYFSSSQIKIFAKSQVD 344
           TPGGLLF     N+QY +++S +L  Y+K L  A    VHC    ++  +++  AK QVD
Sbjct: 352 TPGGLLFKMADANMQYVTSTSFLLLTYAKYLTSAK-TVVHCGGSVYTPGRLRSIAKRQVD 411

[Close]

BLAST of Carg02556 vs. TAIR 10
Match: AT1G71380.1 (cellulase 3 )

HSP 1 Score: 240.0 bits (611), Expect = 2.9e-63
Identity = 155/477 (32.49%), Postives = 217/477 (45.49%), Query Frame = 0


Query: 21  LLSPTLGVGGAAHPPFNYSDALDKAVLFFEGQRSGKLPVAQRVMWRGDSALSDGAHENVN 80
           L S  L   G A+P  NY +AL K++LFF+GQRSG LP  Q++ WR  S LSDG+  +V+
Sbjct: 10  LFSSLLISNGDANP--NYKEALSKSLLFFQGQRSGPLPRGQQISWRASSGLSDGSAAHVD 69

Query: 81  LVGGYYDAGDNVKLVWPMAFTVTLLSWTAVEYENEMSSVMQLQHLRGSVRWGADFILQ-A 140
           L GGYYDAGDNVK   PMAFT T+LSW+A+EY   M    +L++ R ++RW  D++L+ A
Sbjct: 70  LTGGYYDAGDNVKFNLPMAFTTTMLSWSALEYGKRMGP--ELENARVNIRWATDYLLKCA 129

Query: 141 HVSPTALY---------------------------------------------------- 200
             +P  LY                                                    
Sbjct: 130 RATPGKLYVGVGDPNVDHKCWERPEDMDTPRTVYSVSASNPGSDVAAETAAALAAASMVF 189

Query: 201 -----------------TQEF----------------------YKGKRE----------- 260
                              +F                      Y G ++           
Sbjct: 190 RKVDSKYSRLLLATAKDVMQFAIQYQGAYSDSLSSSVCPFYCSYSGYKDELMWGASWLLR 249

Query: 261 -----------------------------------------------LSKYKSDIESFIC 320
                                                            +YK   E+FIC
Sbjct: 250 ATNNPYYANFIKSLGGGDQPDIFSWDNKYAGAYVLLSRRALLNKDSNFEQYKQAAENFIC 309

Query: 321 AVMPNSGSSQIPTTPGGLLFIRDSTNLQYASTSSMVLFMYSKLLNQAHIDGVHCRSKYFS 348
            ++P+S SS    T GGL++    +NLQY ++ + +L  Y+K + +A     +C S    
Sbjct: 310 KILPDSPSSSTQYTQGGLMYKLPQSNLQYVTSITFLLTTYAKYM-KATKHTFNCGSSVIV 369

The following BLAST results are available for this feature:

BLAST of Carg02556 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7036014.1	2.0e-199	100.00	hypothetical protein SDJN02_02814, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6606068.1	6.5e-198	98.85	hypothetical protein SDJN03_03385, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022958537.1	1.1e-178	69.65	endoglucanase-like [Cucurbita moschata]	[more]
KAA0042719.1	2.2e-129	54.26	endoglucanase-like [Cucumis melo var. makuwa]	[more]
XP_008437724.1	1.1e-128	54.26	PREDICTED: endoglucanase-like [Cucumis melo]	[more]

BLAST of Carg02556 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P22503	3.5e-114	46.07	Endoglucanase OS=Phaseolus vulgaris OX=3885 PE=2 SV=2	[more]
Q9SUS0	2.7e-106	45.37	Endoglucanase 20 OS=Arabidopsis thaliana OX=3702 GN=At4g23560 PE=2 SV=1	[more]
Q9SZ90	6.3e-103	43.96	Endoglucanase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g09740 PE=3 SV=2	[more]
Q6ZA06	1.1e-96	42.76	Endoglucanase 20 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU15 PE=2 SV=1	[more]
Q8LQ92	3.1e-70	34.48	Endoglucanase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU8 PE=2 SV=1	[more]

BLAST of Carg02556 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H239	5.6e-179	69.65	Endoglucanase OS=Cucurbita moschata OX=3662 GN=LOC111459740 PE=3 SV=1	[more]
A0A5A7TMY1	1.1e-129	54.26	Endoglucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002170 ...	[more]
A0A1S3AVA7	5.3e-129	54.26	Endoglucanase OS=Cucumis melo OX=3656 GN=LOC103483071 PE=3 SV=1	[more]
A0A6J1DTR0	1.2e-128	54.04	Endoglucanase OS=Momordica charantia OX=3673 GN=LOC111023924 PE=3 SV=1	[more]
A0A6J1DX17	8.4e-127	53.48	Endoglucanase OS=Momordica charantia OX=3673 GN=LOC111023924 PE=3 SV=1	[more]

BLAST of Carg02556 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23560.1	1.9e-107	45.37	glycosyl hydrolase 9B15	[more]
AT4G09740.1	4.4e-104	43.96	glycosyl hydrolase 9B14	[more]
AT1G02800.1	4.6e-69	34.20	cellulase 2	[more]
AT4G02290.1	1.9e-67	32.61	glycosyl hydrolase 9B13	[more]
AT1G71380.1	2.9e-63	32.49	cellulase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012341	Six-hairpin glycosidase-like superfamily	GENE3D	1.50.10.10		coord: 33..152 e-value: 2.3E-43 score: 150.9
IPR012341	Six-hairpin glycosidase-like superfamily	GENE3D	1.50.10.10		coord: 153..347 e-value: 6.4E-52 score: 179.2
IPR001701	Glycoside hydrolase family 9	PFAM	PF00759	Glyco_hydro_9	coord: 155..340 e-value: 1.3E-42 score: 146.8 coord: 38..149 e-value: 1.5E-35 score: 123.5
None	No IPR available	PANTHER	PTHR22298:SF22	ENDOGLUCANASE 18-RELATED	coord: 35..149 coord: 146..346
None	No IPR available	PANTHER	PTHR22298	ENDO-1,4-BETA-GLUCANASE	coord: 35..149 coord: 146..346
IPR018221	Glycoside hydrolase family 9, His active site	PROSITE	PS00592	GH9_2	coord: 244..270
IPR008928	Six-hairpin glycosidase superfamily	SUPERFAMILY	48208	Six-hairpin glycosidases	coord: 35..345

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg02556-RA	Carg02556-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds