Carg02545 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg02545
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	E3 ubiquitin-protein ligase RHA1B-like
Location	Carg_Chr02: 8170627 .. 8171163 (+)
RNA-Seq Expression	Carg02545
Synteny	Carg02545

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCTTCCCTGCAAGCTACACTGAGCTGCTTCTCCCGAAGCTCTTCATCCATGTACTCTCCTTACTCGGCTTCCTCAGGAAGCTCATCTCCCTCTTATTTTGCCTTCTAGGGCTCCAGGATTTTCTTGAACCGGACATTCCTTGGTCTAATCATTCTGATTCTTTTCCGACGGGACCATTGACGGACCTCGATCCTCTTTCCGCTGCTGTTCTCCGTGAGATTCTTCCTGTCGTCAGATTCTCTGAACTTCTGGATCCGCCGGAGAGCTGCGCTGTTTGTCTCTACGAGTTCGAGACCGACGATGAGATCCGCCGCCTCGCGAACTGTCGCCACATCTTCCACCGCGGATGCCTCGATCGGTGGATCGGTTATGGTCAGAGGACCTGCCCGCTGTGCCGGACGGTGTTCATTCCGCCCGATTTGCGGACTGGTGGTTGTAATTTTGACGATAGGCCTTGGGAGGATTCGGAACTCTTCGAAGCACTCCACACTGATTCTTCTTCATCCTCGACGCTTCTCAGCGCTGGTTTGTAG

mRNA sequence

ATGGGCTTCCCTGCAAGCTACACTGAGCTGCTTCTCCCGAAGCTCTTCATCCATGTACTCTCCTTACTCGGCTTCCTCAGGAAGCTCATCTCCCTCTTATTTTGCCTTCTAGGGCTCCAGGATTTTCTTGAACCGGACATTCCTTGGTCTAATCATTCTGATTCTTTTCCGACGGGACCATTGACGGACCTCGATCCTCTTTCCGCTGCTGTTCTCCGTGAGATTCTTCCTGTCGTCAGATTCTCTGAACTTCTGGATCCGCCGGAGAGCTGCGCTGTTTGTCTCTACGAGTTCGAGACCGACGATGAGATCCGCCGCCTCGCGAACTGTCGCCACATCTTCCACCGCGGATGCCTCGATCGGTGGATCGGTTATGGTCAGAGGACCTGCCCGCTGTGCCGGACGGTGTTCATTCCGCCCGATTTGCGGACTGGTGGTTGTAATTTTGACGATAGGCCTTGGGAGGATTCGGAACTCTTCGAAGCACTCCACACTGATTCTTCTTCATCCTCGACGCTTCTCAGCGCTGGTTTGTAG

Coding sequence (CDS)

ATGGGCTTCCCTGCAAGCTACACTGAGCTGCTTCTCCCGAAGCTCTTCATCCATGTACTCTCCTTACTCGGCTTCCTCAGGAAGCTCATCTCCCTCTTATTTTGCCTTCTAGGGCTCCAGGATTTTCTTGAACCGGACATTCCTTGGTCTAATCATTCTGATTCTTTTCCGACGGGACCATTGACGGACCTCGATCCTCTTTCCGCTGCTGTTCTCCGTGAGATTCTTCCTGTCGTCAGATTCTCTGAACTTCTGGATCCGCCGGAGAGCTGCGCTGTTTGTCTCTACGAGTTCGAGACCGACGATGAGATCCGCCGCCTCGCGAACTGTCGCCACATCTTCCACCGCGGATGCCTCGATCGGTGGATCGGTTATGGTCAGAGGACCTGCCCGCTGTGCCGGACGGTGTTCATTCCGCCCGATTTGCGGACTGGTGGTTGTAATTTTGACGATAGGCCTTGGGAGGATTCGGAACTCTTCGAAGCACTCCACACTGATTCTTCTTCATCCTCGACGCTTCTCAGCGCTGGTTTGTAG

Protein sequence

MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGPLTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLDRWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSSTLLSAGL

Homology

[Close]

BLAST of Carg02545 vs. NCBI nr
Match: KAG6606078.1 (Brassinosteroid-responsive RING protein 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036025.1 E3 ubiquitin-protein ligase RHA1B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 373.2 bits (957), Expect = 1.2e-99
Identity = 178/178 (100.00%), Postives = 178/178 (100.00%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120
           LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSSTLLSAGL 179
           RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSSTLLSAGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSSTLLSAGL 178

[Close]

BLAST of Carg02545 vs. NCBI nr
Match: XP_022958594.1 (E3 ubiquitin-protein ligase RHA1B-like [Cucurbita moschata])

HSP 1 Score: 370.2 bits (949), Expect = 1.0e-98
Identity = 177/178 (99.44%), Postives = 177/178 (99.44%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120
           LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSSTLLSAGL 179
           RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDR WEDSELFEALHTDSSSSSTLLSAGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRAWEDSELFEALHTDSSSSSTLLSAGL 178

[Close]

BLAST of Carg02545 vs. NCBI nr
Match: XP_022995549.1 (E3 ubiquitin-protein ligase RHA1B-like [Cucurbita maxima] >XP_023533861.1 E3 ubiquitin-protein ligase RHA1B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 363.6 bits (932), Expect = 9.6e-97
Identity = 177/180 (98.33%), Postives = 177/180 (98.33%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLF LLGLQDFLEPDIPWSNHSDSFPTGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFRLLGLQDFLEPDIPWSNHSDSFPTGP 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120
           LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSS--TLLSAGL 179
           RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSS  TLLSAGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSSTPTLLSAGL 180

[Close]

BLAST of Carg02545 vs. NCBI nr
Match: XP_038906771.1 (E3 ubiquitin-protein ligase RHA1B [Benincasa hispida])

HSP 1 Score: 345.9 bits (886), Expect = 2.1e-91
Identity = 167/180 (92.78%), Postives = 171/180 (95.00%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFPASYTELLLPKLFIHVLS LGFLRKLISLLF LLGLQDFLEPDIPW NH DSFPTGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTGP 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120
            TDLDPLSAAVLREILPVV+FSELLDPPESCAVCLYEFE DDEIRRLANCRHIFHRGCLD
Sbjct: 61  FTDLDPLSAAVLREILPVVKFSELLDPPESCAVCLYEFEADDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTD--SSSSSTLLSAGL 179
           RWIGYGQRTCPLCRTVFIPPDLR+GGCNFDDR WEDSE+FEALHTD  SSSSS+LLSAGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFEALHTDSSSSSSSSLLSAGL 180

[Close]

BLAST of Carg02545 vs. NCBI nr
Match: XP_023549736.1 (E3 ubiquitin-protein ligase RHA1B [Cucurbita pepo subsp. pepo])

HSP 1 Score: 342.0 bits (876), Expect = 3.0e-90
Identity = 165/178 (92.70%), Postives = 171/178 (96.07%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFPASYTELLLPKLFIHVLS+LGFLRKLISLLF LLGLQDFLEPDIPW N SDSF TGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSVLGFLRKLISLLFRLLGLQDFLEPDIPWPNQSDSFTTGP 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120
           LTDLDPLSAAVLREILPVV+FSELLDPPESCAVCLYEFE+DDEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVKFSELLDPPESCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSSTLLSAGL 179
           RWIGYGQRTCPLCRTVFIP DLR+G CNFDDR WEDSE+FEALH+DSSSSSTLLSAGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPGDLRSGVCNFDDRLWEDSEIFEALHSDSSSSSTLLSAGL 178

[Close]

BLAST of Carg02545 vs. ExPASy Swiss-Prot
Match: Q9XF92 (Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH1 PE=2 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 1.7e-43
Identity = 86/161 (53.42%), Postives = 111/161 (68.94%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFP  YTE+ LPKLF+  LS+LGF+R ++  +F  LGL DFLE D  W +++ S+PT  
Sbjct: 1   MGFPVGYTEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPDYT-SYPTRI 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELL----DPPESCAVCLYEFETDDEIRRLANCRHIFHR 120
                P SA ++REILPV++F EL     D PE+CAVCLYEFE + EIR L NCRHIFHR
Sbjct: 61  PETRSPFSALLIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHR 120

Query: 121 GCLDRWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDS 158
            CLDRW+ + Q+TCPLCRT F+P +++     F+ R W  S
Sbjct: 121 SCLDRWMDHDQKTCPLCRTPFVPDEMQE---EFNQRLWAAS 157

[Close]

BLAST of Carg02545 vs. ExPASy Swiss-Prot
Match: Q9SUS5 (E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.0e-24
Identity = 58/146 (39.73%), Postives = 85/146 (58.22%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MG P  + EL +P   +  L ++GF R ++  L   +GL  FL+       H+++  + P
Sbjct: 1   MGLPTDFKELQIPGYVLKTLYVIGFFRDMVDALCPYIGLPSFLD-------HNETSRSDP 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELL-DPPESCAVCLYEFETDDEIRRLANCRHIFHRGCL 120
                  SA +  E++PVVRFS+LL DP + C VCL +F +DD+IR+L  C H+FH  CL
Sbjct: 61  TRLALSTSATLANELIPVVRFSDLLTDPEDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCL 120

Query: 121 DRWI-GYGQRTCPLCRTVFIPPDLRT 145
           DRWI    + TCP+CR  F+P +  T
Sbjct: 121 DRWIVDCNKITCPICRNRFLPEEKST 139

[Close]

BLAST of Carg02545 vs. ExPASy Swiss-Prot
Match: Q9SUS4 (Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RHA1A PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 3.3e-23
Identity = 57/147 (38.78%), Postives = 86/147 (58.50%), Query Frame = 0


Query: 1   MGFPASY-TELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTG 60
           MG P  + TEL +P   + +L ++GF R ++  L   +GL  FL+       H+++    
Sbjct: 1   MGLPEDFITELQIPSYILKILYVIGFFRDIVDALCPYIGLPRFLD-------HNETSAPD 60

Query: 61  PLTDLDPLSAAVLREILPVVRFSEL-LDPPESCAVCLYEFETDDEIRRLANCRHIFHRGC 120
                   SA++  E++PVVRFS+L  DP + C VCL +FE+DD++R+L  C H+FH  C
Sbjct: 61  LTRHALSTSASLANELIPVVRFSDLPTDPEDCCTVCLSDFESDDKVRQLPKCGHVFHHYC 120

Query: 121 LDRWI-GYGQRTCPLCRTVFIPPDLRT 145
           LDRWI  Y +  CP+CR  F+P +  T
Sbjct: 121 LDRWIVDYNKMKCPVCRHRFLPKEKYT 140

[Close]

BLAST of Carg02545 vs. ExPASy Swiss-Prot
Match: Q9LZJ6 (RING-H2 finger protein ATL5 OS=Arabidopsis thaliana OX=3702 GN=ATL5 PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.5e-12
Identity = 47/123 (38.21%), Postives = 60/123 (48.78%), Query Frame = 0


Query: 57  PTGPLTDLDPLSAAVLREILPVVRFSELL--DPPESCAVCLYEFETDDEIRRLANCRHIF 116
           PT   + L PL   VL +I P+  +S      P E C+VCL EFE DDE R L  C H+F
Sbjct: 78  PTQSSSSLSPLDPTVLEKI-PIFVYSVKTHESPLEECSVCLSEFEEDDEGRVLPKCGHVF 137

Query: 117 HRGCLDRWIGYGQRTCPLCRTVFIPPDLRT-----GGCNFDDRPWEDSELFEALHTDSSS 173
           H  C+D W    + +CPLCR    P    T            +P ED+E   +  +D S 
Sbjct: 138 HVDCIDTWF-RSRSSCPLCRAPVQPAQPVTEPEPVAAVFPSVKPIEDTEAGSSSSSDESE 197

[Close]

BLAST of Carg02545 vs. ExPASy Swiss-Prot
Match: Q9P7E1 (Uncharacterized RING finger protein P4H10.07 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBP4H10.07 PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.7e-11
Identity = 27/47 (57.45%), Postives = 32/47 (68.09%), Query Frame = 0


Query: 89  ESCAVCLYEFETDDEIRRLANCRHIFHRGCLDRWIGYGQRTCPLCRT 136
           E C VCL  FE +DE RRL  C H FHR C+D+W+   Q +CPLCRT
Sbjct: 523 ERCLVCLSNFELNDECRRLKQCNHFFHRECIDQWLTSSQNSCPLCRT 569

[Close]

BLAST of Carg02545 vs. ExPASy TrEMBL
Match: A0A6J1H5J7 (E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita moschata OX=3662 GN=LOC111459775 PE=4 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 5.0e-99
Identity = 177/178 (99.44%), Postives = 177/178 (99.44%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120
           LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSSTLLSAGL 179
           RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDR WEDSELFEALHTDSSSSSTLLSAGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRAWEDSELFEALHTDSSSSSTLLSAGL 178

[Close]

BLAST of Carg02545 vs. ExPASy TrEMBL
Match: A0A6J1K664 (E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita maxima OX=3661 GN=LOC111491048 PE=4 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 4.7e-97
Identity = 177/180 (98.33%), Postives = 177/180 (98.33%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLF LLGLQDFLEPDIPWSNHSDSFPTGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFRLLGLQDFLEPDIPWSNHSDSFPTGP 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120
           LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSS--TLLSAGL 179
           RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSS  TLLSAGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSSTPTLLSAGL 180

[Close]

BLAST of Carg02545 vs. ExPASy TrEMBL
Match: A0A6J1I1X7 (E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita maxima OX=3661 GN=LOC111469101 PE=4 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 2.3e-88
Identity = 163/178 (91.57%), Postives = 169/178 (94.94%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFPASYTELLLPKLFIHVLS+LGFLRKLISLLF LLGLQDFLEPDIPW N SDSF TGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSILGFLRKLISLLFRLLGLQDFLEPDIPWPNQSDSFTTGP 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120
           LTDLDPLSAAVLREILPVV+FSELLDPPESCAVCLYEFE+DDEIR LANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVKFSELLDPPESCAVCLYEFESDDEIRHLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSSTLLSAGL 179
           RWIGYGQRTCPLCRTVFIP DLR+G CNFDDR WEDSE+FEALH+D SSSSTLLSAGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPGDLRSGVCNFDDRLWEDSEIFEALHSD-SSSSTLLSAGL 177

[Close]

BLAST of Carg02545 vs. ExPASy TrEMBL
Match: A0A6J1EQ05 (E3 ubiquitin-protein ligase RHA1B OS=Cucurbita moschata OX=3662 GN=LOC111435580 PE=4 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 1.2e-87
Identity = 163/178 (91.57%), Postives = 169/178 (94.94%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFPASYTELLLPKLFIHVLS+LGFLRKLISLLF LLGLQDFLEPDI W N SDSF TGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSVLGFLRKLISLLFRLLGLQDFLEPDILWPNQSDSFTTGP 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120
           LTDLDPLSAAVLREILPVV+FSELLDPPESCAVCLYEFE+DDEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVKFSELLDPPESCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSSTLLSAGL 179
           RWIGYGQRTCPLCRTVFIP DLR+G CNFDDR WEDSE+FEALH+D SSSSTLLSAGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPGDLRSGVCNFDDRLWEDSEIFEALHSD-SSSSTLLSAGL 177

[Close]

BLAST of Carg02545 vs. ExPASy TrEMBL
Match: A0A1S3AU97 (E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo OX=3656 GN=LOC103482771 PE=4 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 1.3e-83
Identity = 152/179 (84.92%), Postives = 163/179 (91.06%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFPA+YTELLLPKLFIH+LS LGFLRKLIS LF L GLQDFLEPDIPW N SDSFPT P
Sbjct: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPSDSFPTSP 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120
            T LDPLSAA+LREILPVV+FS+LLDPP+ CAVCLYEFE+DDEIRRLANCRHIFHRGCLD
Sbjct: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRT-GGCNFDDRPWEDSELFEALHTDSSSSSTLLSAGL 179
           RWIGYGQRTCPLCRTVFIPPDLR+ GGCNFDDR WEDSE+FE LHTDSSSSS+  S+ L
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGGCNFDDRLWEDSEIFEPLHTDSSSSSSSSSSNL 179

[Close]

BLAST of Carg02545 vs. TAIR 10
Match: AT1G63840.1 (RING/U-box superfamily protein )

HSP 1 Score: 184.1 bits (466), Expect = 9.7e-47
Identity = 100/173 (57.80%), Postives = 119/173 (68.79%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFP  Y+ELLLPK+F ++LS LG +RKLIS +F ++GL DFLEP+      S S+P  P
Sbjct: 1   MGFPVGYSELLLPKIFFYLLSFLGLIRKLISTMFKIIGLPDFLEPE----PVSTSWPDPP 60

Query: 61  LTDLDPLSAAVLR-EILPVVRFSELLDP-PESCAVCLYEFETDDEIRRLANCRHIFHRGC 120
            T   P SAA+L  E+LPVVRFS++  P  E CAVCLY+FE DDEIRRL NCRHIFHRGC
Sbjct: 61  PTLTKPDSAAILAGEMLPVVRFSDINRPESECCAVCLYDFENDDEIRRLTNCRHIFHRGC 120

Query: 121 LDRW-IGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSS 171
           LDRW +GY Q TCPLCRT FIP  L+     F+ R W  S    +   D SSS
Sbjct: 121 LDRWMMGYNQMTCPLCRTQFIPDHLQ---LEFNQRLWSQSSAVSSQLLDESSS 166

[Close]

BLAST of Carg02545 vs. TAIR 10
Match: AT3G61460.1 (brassinosteroid-responsive RING-H2 )

HSP 1 Score: 177.2 bits (448), Expect = 1.2e-44
Identity = 86/161 (53.42%), Postives = 111/161 (68.94%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFP  YTE+ LPKLF+  LS+LGF+R ++  +F  LGL DFLE D  W +++ S+PT  
Sbjct: 1   MGFPVGYTEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPDYT-SYPTRI 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELL----DPPESCAVCLYEFETDDEIRRLANCRHIFHR 120
                P SA ++REILPV++F EL     D PE+CAVCLYEFE + EIR L NCRHIFHR
Sbjct: 61  PETRSPFSALLIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHR 120

Query: 121 GCLDRWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDS 158
            CLDRW+ + Q+TCPLCRT F+P +++     F+ R W  S
Sbjct: 121 SCLDRWMDHDQKTCPLCRTPFVPDEMQE---EFNQRLWAAS 157

[Close]

BLAST of Carg02545 vs. TAIR 10
Match: AT5G41400.1 (RING/U-box superfamily protein )

HSP 1 Score: 167.5 bits (423), Expect = 9.4e-42
Identity = 92/174 (52.87%), Postives = 112/174 (64.37%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MG+P  YTELLLP++F+H+LSLLG +R LI   F +LGL DFLE D   S+ S   P   
Sbjct: 1   MGYPVGYTELLLPRIFLHLLSLLGLIRTLIDTGFRILGLPDFLESDPVSSSSSWLEPPYM 60

Query: 61  LTDLD---------PLSAAVLREILPVVRFSELLDP-----PESCAVCLYEFETDDEIRR 120
            T            P++A +  EILPV+RFSEL  P      + CAVCL+EFE DDEIRR
Sbjct: 61  STAAHHHQESSFFFPVAARLAGEILPVIRFSELTRPGFGSGSDCCAVCLHEFENDDEIRR 120

Query: 121 LANCRHIFHRGCLDRW-IGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSEL 160
           L NC+HIFHR CLDRW +GY Q TCPLCRT FI  +L+     F+ R W +SEL
Sbjct: 121 LTNCQHIFHRSCLDRWMMGYNQMTCPLCRTPFISDELQVA---FNQRVWSESEL 171

[Close]

BLAST of Carg02545 vs. TAIR 10
Match: AT4G11360.1 (RING-H2 finger A1B )

HSP 1 Score: 113.2 bits (282), Expect = 2.1e-25
Identity = 58/146 (39.73%), Postives = 85/146 (58.22%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MG P  + EL +P   +  L ++GF R ++  L   +GL  FL+       H+++  + P
Sbjct: 1   MGLPTDFKELQIPGYVLKTLYVIGFFRDMVDALCPYIGLPSFLD-------HNETSRSDP 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELL-DPPESCAVCLYEFETDDEIRRLANCRHIFHRGCL 120
                  SA +  E++PVVRFS+LL DP + C VCL +F +DD+IR+L  C H+FH  CL
Sbjct: 61  TRLALSTSATLANELIPVVRFSDLLTDPEDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCL 120

Query: 121 DRWI-GYGQRTCPLCRTVFIPPDLRT 145
           DRWI    + TCP+CR  F+P +  T
Sbjct: 121 DRWIVDCNKITCPICRNRFLPEEKST 139

[Close]

BLAST of Carg02545 vs. TAIR 10
Match: AT4G11370.1 (RING-H2 finger A1A )

HSP 1 Score: 109.8 bits (273), Expect = 2.3e-24
Identity = 57/147 (38.78%), Postives = 86/147 (58.50%), Query Frame = 0


Query: 1   MGFPASY-TELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTG 60
           MG P  + TEL +P   + +L ++GF R ++  L   +GL  FL+       H+++    
Sbjct: 1   MGLPEDFITELQIPSYILKILYVIGFFRDIVDALCPYIGLPRFLD-------HNETSAPD 60

Query: 61  PLTDLDPLSAAVLREILPVVRFSEL-LDPPESCAVCLYEFETDDEIRRLANCRHIFHRGC 120
                   SA++  E++PVVRFS+L  DP + C VCL +FE+DD++R+L  C H+FH  C
Sbjct: 61  LTRHALSTSASLANELIPVVRFSDLPTDPEDCCTVCLSDFESDDKVRQLPKCGHVFHHYC 120

Query: 121 LDRWI-GYGQRTCPLCRTVFIPPDLRT 145
           LDRWI  Y +  CP+CR  F+P +  T
Sbjct: 121 LDRWIVDYNKMKCPVCRHRFLPKEKYT 140

The following BLAST results are available for this feature:

BLAST of Carg02545 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6606078.1	1.2e-99	100.00	Brassinosteroid-responsive RING protein 1, partial [Cucurbita argyrosperma subsp...	[more]
XP_022958594.1	1.0e-98	99.44	E3 ubiquitin-protein ligase RHA1B-like [Cucurbita moschata]	[more]
XP_022995549.1	9.6e-97	98.33	E3 ubiquitin-protein ligase RHA1B-like [Cucurbita maxima] >XP_023533861.1 E3 ubi...	[more]
XP_038906771.1	2.1e-91	92.78	E3 ubiquitin-protein ligase RHA1B [Benincasa hispida]	[more]
XP_023549736.1	3.0e-90	92.70	E3 ubiquitin-protein ligase RHA1B [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg02545 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XF92	1.7e-43	53.42	Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH...	[more]
Q9SUS5	3.0e-24	39.73	E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 ...	[more]
Q9SUS4	3.3e-23	38.78	Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q9LZJ6	1.5e-12	38.21	RING-H2 finger protein ATL5 OS=Arabidopsis thaliana OX=3702 GN=ATL5 PE=2 SV=1	[more]
Q9P7E1	1.7e-11	57.45	Uncharacterized RING finger protein P4H10.07 OS=Schizosaccharomyces pombe (strai...	[more]

BLAST of Carg02545 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H5J7	5.0e-99	99.44	E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A6J1K664	4.7e-97	98.33	E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita maxima OX=3661 GN=LOC1114910...	[more]
A0A6J1I1X7	2.3e-88	91.57	E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita maxima OX=3661 GN=LOC1114691...	[more]
A0A6J1EQ05	1.2e-87	91.57	E3 ubiquitin-protein ligase RHA1B OS=Cucurbita moschata OX=3662 GN=LOC111435580 ...	[more]
A0A1S3AU97	1.3e-83	84.92	E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo OX=3656 GN=LOC103482771 PE=4 S...	[more]

BLAST of Carg02545 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G63840.1	9.7e-47	57.80	RING/U-box superfamily protein	[more]
AT3G61460.1	1.2e-44	53.42	brassinosteroid-responsive RING-H2	[more]
AT5G41400.1	9.4e-42	52.87	RING/U-box superfamily protein	[more]
AT4G11360.1	2.1e-25	39.73	RING-H2 finger A1B	[more]
AT4G11370.1	2.3e-24	38.78	RING-H2 finger A1A	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 91..133 e-value: 3.1E-6 score: 36.7
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 90..134 e-value: 1.4E-13 score: 51.0
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 91..134 score: 12.459772
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 85..137 e-value: 1.1E-17 score: 65.3
None	No IPR available	PANTHER	PTHR45969:SF49	SUBFAMILY NOT NAMED	coord: 1..143
None	No IPR available	PANTHER	PTHR45969	RING ZINC FINGER PROTEIN-RELATED	coord: 1..143
None	No IPR available	CDD	cd16448	RING-H2	coord: 91..134 e-value: 1.39578E-13 score: 60.1583
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 72..140

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg02545-RA	Carg02545-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding