Carg02443 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg02443
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	L-lactate dehydrogenase
Location	Carg_Chr02: 8685461 .. 8687092 (+)
RNA-Seq Expression	Carg02443
Synteny	Carg02443

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGAAGAGTCCGTCAGCATCCGCCTTGGGTCCCGGCGGCCTCGACCTTTCACAGGCCTTCTTCAAGCCCATCCCTGACGCCTCTCCTCCCTCGACCTCCAAGGGCAACACTAAAATCTCTGTTATTGGCGCTGGTAACGTCGGCATGGCCATCGCTCAGACCATCCTCACTCAAGATTTGGCCGATGAACTCGTCCTCGTCGACGCCAAGCCTAACAAGCTCCGCGGCGAAATGCTCGACCTCCAACACGCCGCCGCCTTCCTCCCCCGCACCAAAATCAGCGCCTCCACCGACTACTCTATCACCGCTGGATCCGATCTCTGCATTGTCACAGCCGGCGCTCGTCAGATCGCCGGCGAATCTAGGTTGAATCTCCTGCAGAGGAACGTTGCTCTATTCCAGAAGATAGTTCCGCCGCTTGTTCAGTACTCTCCCGATACGATATTGTTAATCGTTTCGAATCCGGTGGATGTTTTGACCTATGTTGCTTGGAAGCTCTCTGGTTTTCCCTCGAATCGAGTTATTGGATCAGGAACAAATCTGGACTCGTCTAGGTTTAGGTTTCTCATTGCCGATCATCTCGACGTCAACGCTCAGGACGTTCAGGTCAGTTTTCCGATCAACATTTCTTCAACCTTTTTGAATCGATTAGTGCCTTATTGTTGGTTTGCTGTTGTCGAAGTAGTTTTGATTTTATCGTTTTTGGTCGATTGATGTAAAACATGGCTGGTAGTAAGATTGACATTGAAGAAATCCGTGGGAGAAGTTGAGGAAATTGAATTCGATGTTTCTAAAAACTTCCATGAAATTTGGAAAGTGAAATTCCTAATTTCTGCCAACTCACACTCAATAATTGACAAACCTTTAATTGAAACACTAGATGAATTTCTACAAACTAGCATTGATATTTAGATCATGAAGCTAAAAAAAAAGGGATCTCATATATGTTTGAAGGCATACATAGTGGGGGAGCACGGCGACAGCTCAGTGGCGCTCTGGTCAAGCATAAGCGTGGGCGGGGTGCCAATACTAAGCTTTCTAAAGAGCCAACAAATTGCGTACGAGAAGGAAACATTAGAGAACATTCACAAGGAGGTGATAGGGGGGGCGTATGAAGTAATACGCCTAAAGGGCTACACGTCATGGGCGATCGGGTACTCGGTGGCTAGCCTTGCCCGATCACTCCTTCGTGACCAGCAGAGGATCCACCCTGTATCCGTCCTCGCAAAAGGGTTCTACGGCATTGATGGGGGCGACCTGTTCCTCAGCCTGCCCGCACGGCTAGGGCGAGGCGGAGTATTGGGGATTACCAATGTGCATCTAACCACGGAGGAGATGCGGCGGCTGCAGGACTCGGCCAACACCATCTTGCAGGTGCAATCGGAGTTGGGGATTTGAAAACTTCAGCACTTGCTAACCATGGATGATGATATGAATGTTTTTTATGATATATTTTGATGGGTTGTTGTGATGATCTGAATGTTTTTTATGATAGATTTTGATGATATATTTTGATGGGTTGCTGTGCTAACCATGGATTATGAACGTGTAGGTCATTATTTAGATTTTAATTATTATTATTATTTTTTACTTTAATAAAATTTCAAAAATAAAACCCTACTTCATATA

mRNA sequence

ATGCAGAAGAGTCCGTCAGCATCCGCCTTGGGTCCCGGCGGCCTCGACCTTTCACAGGCCTTCTTCAAGCCCATCCCTGACGCCTCTCCTCCCTCGACCTCCAAGGGCAACACTAAAATCTCTGTTATTGGCGCTGGTAACGTCGGCATGGCCATCGCTCAGACCATCCTCACTCAAGATTTGGCCGATGAACTCGTCCTCGTCGACGCCAAGCCTAACAAGCTCCGCGGCGAAATGCTCGACCTCCAACACGCCGCCGCCTTCCTCCCCCGCACCAAAATCAGCGCCTCCACCGACTACTCTATCACCGCTGGATCCGATCTCTGCATTGTCACAGCCGGCGCTCGTCAGATCGCCGGCGAATCTAGGTTGAATCTCCTGCAGAGGAACGTTGCTCTATTCCAGAAGATAGTTCCGCCGCTTGTTCAGTACTCTCCCGATACGATATTGTTAATCGTTTCGAATCCGGTGGATGTTTTGACCTATGTTGCTTGGAAGCTCTCTGGTTTTCCCTCGAATCGAGTTATTGGATCAGGAACAAATCTGGACTCGTCTAGGTTTAGGTTTCTCATTGCCGATCATCTCGACGTCAACGCTCAGGACGTTCAGGCATACATAGTGGGGGAGCACGGCGACAGCTCAGTGGCGCTCTGGTCAAGCATAAGCGTGGGCGGGGTGCCAATACTAAGCTTTCTAAAGAGCCAACAAATTGCGTACGAGAAGGAAACATTAGAGAACATTCACAAGGAGGTGATAGGGGGGGCGTATGAAGTAATACGCCTAAAGGGCTACACGTCATGGGCGATCGGGTACTCGGTGGCTAGCCTTGCCCGATCACTCCTTCGTGACCAGCAGAGGATCCACCCTGTATCCGTCCTCGCAAAAGGGTTCTACGGCATTGATGGGGGCGACCTGTTCCTCAGCCTGCCCGCACGGCTAGGGCGAGGCGGAGTATTGGGGATTACCAATGTGCATCTAACCACGGAGGAGATGCGGCGGCTGCAGGACTCGGCCAACACCATCTTGCAGGTGCAATCGGAGTTGGGGATTTGAAAACTTCAGCACTTGCTAACCATGGATGATGATATGAATGTTTTTTATGATATATTTTGATGGGTTGTTGTGATGATCTGAATGTTTTTTATGATAGATTTTGATGATATATTTTGATGGGTTGCTGTGCTAACCATGGATTATGAACGTGTAGGTCATTATTTAGATTTTAATTATTATTATTATTTTTTACTTTAATAAAATTTCAAAAATAAAACCCTACTTCATATA

Coding sequence (CDS)

ATGCAGAAGAGTCCGTCAGCATCCGCCTTGGGTCCCGGCGGCCTCGACCTTTCACAGGCCTTCTTCAAGCCCATCCCTGACGCCTCTCCTCCCTCGACCTCCAAGGGCAACACTAAAATCTCTGTTATTGGCGCTGGTAACGTCGGCATGGCCATCGCTCAGACCATCCTCACTCAAGATTTGGCCGATGAACTCGTCCTCGTCGACGCCAAGCCTAACAAGCTCCGCGGCGAAATGCTCGACCTCCAACACGCCGCCGCCTTCCTCCCCCGCACCAAAATCAGCGCCTCCACCGACTACTCTATCACCGCTGGATCCGATCTCTGCATTGTCACAGCCGGCGCTCGTCAGATCGCCGGCGAATCTAGGTTGAATCTCCTGCAGAGGAACGTTGCTCTATTCCAGAAGATAGTTCCGCCGCTTGTTCAGTACTCTCCCGATACGATATTGTTAATCGTTTCGAATCCGGTGGATGTTTTGACCTATGTTGCTTGGAAGCTCTCTGGTTTTCCCTCGAATCGAGTTATTGGATCAGGAACAAATCTGGACTCGTCTAGGTTTAGGTTTCTCATTGCCGATCATCTCGACGTCAACGCTCAGGACGTTCAGGCATACATAGTGGGGGAGCACGGCGACAGCTCAGTGGCGCTCTGGTCAAGCATAAGCGTGGGCGGGGTGCCAATACTAAGCTTTCTAAAGAGCCAACAAATTGCGTACGAGAAGGAAACATTAGAGAACATTCACAAGGAGGTGATAGGGGGGGCGTATGAAGTAATACGCCTAAAGGGCTACACGTCATGGGCGATCGGGTACTCGGTGGCTAGCCTTGCCCGATCACTCCTTCGTGACCAGCAGAGGATCCACCCTGTATCCGTCCTCGCAAAAGGGTTCTACGGCATTGATGGGGGCGACCTGTTCCTCAGCCTGCCCGCACGGCTAGGGCGAGGCGGAGTATTGGGGATTACCAATGTGCATCTAACCACGGAGGAGATGCGGCGGCTGCAGGACTCGGCCAACACCATCTTGCAGGTGCAATCGGAGTTGGGGATTTGA

Protein sequence

MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQDLADELVLVDAKPNKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAGESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQIAYEKETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGIDGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI

Homology

[Close]

BLAST of Carg02443 vs. NCBI nr
Match: KAG7036127.1 (hypothetical protein SDJN02_02927, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 676.8 bits (1745), Expect = 1.0e-190
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0


Query: 1   MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD 60
           MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LADELVLVDAKPNKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LADELVLVDAKPNKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG
Sbjct: 61  LADELVLVDAKPNKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQIAYE 240

Query: 241 KETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI 300
           KETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI
Sbjct: 241 KETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI 351
           DGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI
Sbjct: 301 DGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI 350

[Close]

BLAST of Carg02443 vs. NCBI nr
Match: XP_023534013.1 (L-lactate dehydrogenase A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 673.3 bits (1736), Expect = 1.1e-189
Identity = 347/350 (99.14%), Postives = 350/350 (100.00%), Query Frame = 0


Query: 1   MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD 60
           MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LADELVLVDAKPNKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LADELVLVDAKP+KLRGEMLDLQHAAAFLPRTKI+ASTDYSITAGSDLCIVTAGARQIAG
Sbjct: 61  LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKINASTDYSITAGSDLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQYSPDTILL+VSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQYSPDTILLVVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQIAYE 240

Query: 241 KETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI 300
           KETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI
Sbjct: 241 KETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI 351
           DGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI
Sbjct: 301 DGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI 350

[Close]

BLAST of Carg02443 vs. NCBI nr
Match: XP_022958393.1 (L-lactate dehydrogenase A-like [Cucurbita moschata])

HSP 1 Score: 673.3 bits (1736), Expect = 1.1e-189
Identity = 348/350 (99.43%), Postives = 349/350 (99.71%), Query Frame = 0


Query: 1   MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD 60
           MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LADELVLVDAKPNKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LADELVLVDAKP+KLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVT GARQIAG
Sbjct: 61  LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTVGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQIAYE 240

Query: 241 KETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI 300
           KETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI
Sbjct: 241 KETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI 351
           DGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI
Sbjct: 301 DGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI 350

[Close]

BLAST of Carg02443 vs. NCBI nr
Match: KAG6606181.1 (hypothetical protein SDJN03_03498, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 671.4 bits (1731), Expect = 4.2e-189
Identity = 347/350 (99.14%), Postives = 349/350 (99.71%), Query Frame = 0


Query: 1   MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD 60
           + KSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD
Sbjct: 106 LYKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD 165

Query: 61  LADELVLVDAKPNKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LADELVLVDAKP+KLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG
Sbjct: 166 LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 225

Query: 121 ESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 226 ESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 285

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQIAYE
Sbjct: 286 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQIAYE 345

Query: 241 KETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI 300
           KETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI
Sbjct: 346 KETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI 405

Query: 301 DGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI 351
           DGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI
Sbjct: 406 DGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI 455

[Close]

BLAST of Carg02443 vs. NCBI nr
Match: XP_022995851.1 (L-lactate dehydrogenase A-like [Cucurbita maxima])

HSP 1 Score: 662.9 bits (1709), Expect = 1.5e-186
Identity = 343/350 (98.00%), Postives = 346/350 (98.86%), Query Frame = 0


Query: 1   MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD 60
           MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LADELVLVDAKPNKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L DELVLVDAKP+KLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG
Sbjct: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQYSPDT LLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQYSPDTKLLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLK+QQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240

Query: 241 KETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI 300
           KETLENIHKEVI GAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI
Sbjct: 241 KETLENIHKEVIEGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI 351
           DG DLFLSLPARLGRGGV+GITNVHLTTEEMRRLQDSANTILQVQSELGI
Sbjct: 301 DGDDLFLSLPARLGRGGVMGITNVHLTTEEMRRLQDSANTILQVQSELGI 350

[Close]

BLAST of Carg02443 vs. ExPASy Swiss-Prot
Match: P22988 (L-lactate dehydrogenase A OS=Hordeum vulgare OX=4513 PE=1 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 5.7e-128
Identity = 241/348 (69.25%), Postives = 285/348 (81.90%), Query Frame = 0


Query: 1   MQKSPSASALGPGGLDLSQAFFKPIPDASP--PSTS----KGNTKISVIGAGNVGMAIAQ 60
           M K+ S S LG      S  FF+P+ D  P  P++S    +  TKISVIGAGNVGMAIAQ
Sbjct: 1   MHKASSLSELGFDAGGASSGFFRPVADGCPATPTSSAVPHRRLTKISVIGAGNVGMAIAQ 60

Query: 61  TILTQDLADELVLVDAKPNKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAG 120
           TILTQ+LADE+ LVDA P+KLRGE LDLQHAAAFLPR +IS  TD ++T  SDL IVTAG
Sbjct: 61  TILTQNLADEIALVDALPDKLRGEALDLQHAAAFLPRVRIS-GTDAAVTKNSDLVIVTAG 120

Query: 121 ARQIAGESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNR 180
           ARQI GE+RLNLLQRNVAL++KIVPP+ ++SPD +LL+VSNPVDVLTYVAWKLSGFP++R
Sbjct: 121 ARQIPGETRLNLLQRNVALYRKIVPPVAEHSPDALLLVVSNPVDVLTYVAWKLSGFPASR 180

Query: 181 VIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKS 240
           VIGSGTNLDSSRFRFLIA+HLDVNAQDVQAY+VGEHGDSSVA+WSSISVGG+P    L+ 
Sbjct: 181 VIGSGTNLDSSRFRFLIAEHLDVNAQDVQAYMVGEHGDSSVAIWSSISVGGMPAFKSLRD 240

Query: 241 QQIAYEKETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLA 300
              ++++  LE I + V+GGAYEVI LKGYTSWAIGYSVASLA SLLRDQ+R+HPVSVLA
Sbjct: 241 SHRSFDEAALEGIRRAVVGGAYEVIGLKGYTSWAIGYSVASLAASLLRDQRRVHPVSVLA 300

Query: 301 KGFYGI-DGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTI 342
            GF+GI DG ++FLSLPARLGRGG+LG+  + LT  E  +L+ SA T+
Sbjct: 301 SGFHGISDGHEVFLSLPARLGRGGILGVAEMDLTEAEAAQLRRSAKTL 347

[Close]

BLAST of Carg02443 vs. ExPASy Swiss-Prot
Match: P22989 (L-lactate dehydrogenase B (Fragment) OS=Hordeum vulgare OX=4513 PE=1 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 1.5e-125
Identity = 231/342 (67.54%), Postives = 279/342 (81.58%), Query Frame = 0


Query: 16  DLSQAFFKPIPDASP------PSTSKGNTKISVIGAGNVGMAIAQTILTQDLADELVLVD 75
           D S  FF+ + D  P       +  +  TK+SVIGAGNVGMAIAQTILTQ+LADE+ LVD
Sbjct: 3   DASSGFFRAVADGCPITHTSCSAPHRRLTKVSVIGAGNVGMAIAQTILTQNLADEIALVD 62

Query: 76  AKPNKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAGESRLNLLQR 135
           A P+KLRGE LDLQHAAAFLPR +I + TD ++T  SDL +VTAGARQI GE+RLNLLQR
Sbjct: 63  ALPDKLRGEALDLQHAAAFLPRVRIVSGTDAAVTKNSDLIVVTAGARQIPGETRLNLLQR 122

Query: 136 NVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGTNLDSSRFRF 195
           NVAL++KIVPP+ ++SPD +LL+VSNPVDVLTYVAWKLSGFP++RVIGSGTNLDSSRFRF
Sbjct: 123 NVALYRKIVPPVAEHSPDALLLVVSNPVDVLTYVAWKLSGFPASRVIGSGTNLDSSRFRF 182

Query: 196 LIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQIAYEKETLENIHK 255
           L+A+HLDV+AQDVQAY+VGEHGDSSVA+WSSISVGG+P L  L+    ++++  LE I +
Sbjct: 183 LVAEHLDVSAQDVQAYMVGEHGDSSVAIWSSISVGGMPALKSLRDSHRSFDEAALEGIRR 242

Query: 256 EVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI-DGGDLFLS 315
            V+GGAYEVI LKGYTSWAIGYSVASLA SLLRDQ+R+HPVSVLA GF+GI DG ++FLS
Sbjct: 243 AVVGGAYEVIGLKGYTSWAIGYSVASLATSLLRDQRRVHPVSVLAAGFHGISDGHEVFLS 302

Query: 316 LPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI 351
           LPARLGR GVLG+  + LT  E  +L+ SA T+ +    LG+
Sbjct: 303 LPARLGRAGVLGVAEMDLTEAEAAQLRRSAKTLWENCQLLGL 344

[Close]

BLAST of Carg02443 vs. ExPASy Swiss-Prot
Match: P29038 (L-lactate dehydrogenase OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 3.9e-121
Identity = 235/355 (66.20%), Postives = 279/355 (78.59%), Query Frame = 0


Query: 1   MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGN----TKISVIGAGNVGMAIAQTI 60
           M+K+ S S LG    D S  FF+P+   S   TS+ +    TK+SVIGAGNVGMAIAQTI
Sbjct: 1   MKKATSLSELGFDAGDASSGFFRPVSGDSSTPTSQHHRRRLTKVSVIGAGNVGMAIAQTI 60

Query: 61  LTQDLADELVLVDAKPNKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGAR 120
           LT+DLADE+ LVDA P+KLRGEMLDLQHAAAFLPRT++ + TD S+T GSDL IVTAGAR
Sbjct: 61  LTRDLADEIALVDAVPDKLRGEMLDLQHAAAFLPRTRLVSGTDMSVTRGSDLVIVTAGAR 120

Query: 121 QIAGESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVI 180
           QI GE+RL+LLQRNVALF+KIVPPL + S D +LL+VSNPVDVLTYVAWKLSGFP++RVI
Sbjct: 121 QIQGETRLDLLQRNVALFRKIVPPLAEQSHDALLLVVSNPVDVLTYVAWKLSGFPASRVI 180

Query: 181 GSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQ 240
           GSGTNLDSSRFRFL+A+HLDVNAQDVQAY+VGEHGDSSVA+WSS+SV G+P+L  L+   
Sbjct: 181 GSGTNLDSSRFRFLLAEHLDVNAQDVQAYMVGEHGDSSVAVWSSVSVAGMPVLKSLQESH 240

Query: 241 IAYEKETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKG 300
             +++E LE I + V+  AYEVI LKGYTSWAIGYSVASLA SLLRDQ+RIHPVSVLA+G
Sbjct: 241 RCFDEEALEGIRRAVVDSAYEVISLKGYTSWAIGYSVASLAASLLRDQRRIHPVSVLARG 300

Query: 301 FYGI-DGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI 351
           F+GI DG     + P R  R    G   + LT EE +RL+ SA TI +    LG+
Sbjct: 301 FHGIPDGTTSSSACPPRRPRRRP-GRREMELTEEEAKRLRRSAKTIWENCQLLGL 354

[Close]

BLAST of Carg02443 vs. ExPASy Swiss-Prot
Match: Q9XT87 (L-lactate dehydrogenase A chain OS=Monodelphis domestica OX=13616 GN=LDHA PE=2 SV=3)

HSP 1 Score: 346.3 bits (887), Expect = 4.1e-94
Identity = 172/312 (55.13%), Postives = 234/312 (75.00%), Query Frame = 0


Query: 37  NTKISVIGAGNVGMAIAQTILTQDLADELVLVDAKPNKLRGEMLDLQHAAAFLPRTKISA 96
           + KI+V+G G VGMA A +IL +DLADEL LVD   +KL+GEM+DLQH + FL   KI +
Sbjct: 20  HNKITVVGVGAVGMACAISILMKDLADELALVDVIEDKLKGEMMDLQHGSLFLKTPKIVS 79

Query: 97  STDYSITAGSDLCIVTAGARQIAGESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNP 156
           S DY++TA S L ++TAGARQ  GESRLNL+QRNV +F+ I+P +V+YSP+  LL+VSNP
Sbjct: 80  SKDYAVTANSKLVVITAGARQQEGESRLNLVQRNVNIFKFIIPNIVKYSPNCKLLVVSNP 139

Query: 157 VDVLTYVAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVA 216
           VD+LTYVAWKLSGFP NRVIGSG NLDS+RFR+L+ + L +++     +I+GEHGDSSV 
Sbjct: 140 VDILTYVAWKLSGFPKNRVIGSGCNLDSARFRYLMGEKLGIHSSSCHGWILGEHGDSSVP 199

Query: 217 LWSSISVGGVPILSFLKSQQIAYEKETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASL 276
           +WS ++V GV + S   +     + E  +++HK+V+  AYEVI+LKGYTSWAIG SVA L
Sbjct: 200 VWSGVNVAGVSLKSLHPALGTDSDSEQWKDVHKQVVESAYEVIKLKGYTSWAIGLSVADL 259

Query: 277 ARSLLRDQQRIHPVSVLAKGFYGIDGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQD 336
           A S++++ +R+HP+S + KG YGI+  D+FLS+P  LG+ G+  +  V LTTEE  RL+ 
Sbjct: 260 AESIMKNLRRVHPISTMIKGLYGIN-EDVFLSVPCILGQNGISDVVKVTLTTEEESRLKQ 319

Query: 337 SANTILQVQSEL 349
           SA+T+  +Q EL
Sbjct: 320 SADTLWGIQKEL 330

[Close]

BLAST of Carg02443 vs. ExPASy Swiss-Prot
Match: P06151 (L-lactate dehydrogenase A chain OS=Mus musculus OX=10090 GN=Ldha PE=1 SV=3)

HSP 1 Score: 345.1 bits (884), Expect = 9.1e-94
Identity = 174/310 (56.13%), Postives = 229/310 (73.87%), Query Frame = 0


Query: 39  KISVIGAGNVGMAIAQTILTQDLADELVLVDAKPNKLRGEMLDLQHAAAFLPRTKISAST 98
           KI+V+G G VGMA A +IL +DLADEL LVD   +KL+GEM+DLQH + FL   KI +S 
Sbjct: 22  KITVVGVGAVGMACAISILMKDLADELALVDVMEDKLKGEMMDLQHGSLFLKTPKIVSSK 81

Query: 99  DYSITAGSDLCIVTAGARQIAGESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVD 158
           DY +TA S L I+TAGARQ  GESRLNL+QRNV +F+ I+P +V+YSP   LLIVSNPVD
Sbjct: 82  DYCVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNIVKYSPHCKLLIVSNPVD 141

Query: 159 VLTYVAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALW 218
           +LTYVAWK+SGFP NRVIGSG NLDS+RFR+L+ + L V+A     +++GEHGDSSV +W
Sbjct: 142 ILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHALSCHGWVLGEHGDSSVPVW 201

Query: 219 SSISVGGVPILSFLKSQQIAYEKETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLAR 278
           S ++V GV + S         +KE  + +HK+V+  AYEVI+LKGYTSWAIG SVA LA 
Sbjct: 202 SGVNVAGVSLKSLNPELGTDADKEQWKEVHKQVVDSAYEVIKLKGYTSWAIGLSVADLAE 261

Query: 279 SLLRDQQRIHPVSVLAKGFYGIDGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSA 338
           S++++ +R+HP+S + KG YGI+  D+FLS+P  LG+ G+  +  V LT EE  RL+ SA
Sbjct: 262 SIMKNLRRVHPISTMIKGLYGIN-EDVFLSVPCILGQNGISDVVKVTLTPEEEARLKKSA 321

Query: 339 NTILQVQSEL 349
           +T+  +Q EL
Sbjct: 322 DTLWGIQKEL 330

[Close]

BLAST of Carg02443 vs. ExPASy TrEMBL
Match: A0A6J1H2Z1 (L-lactate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111459629 PE=3 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 5.4e-190
Identity = 348/350 (99.43%), Postives = 349/350 (99.71%), Query Frame = 0


Query: 1   MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD 60
           MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LADELVLVDAKPNKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LADELVLVDAKP+KLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVT GARQIAG
Sbjct: 61  LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTVGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQIAYE 240

Query: 241 KETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI 300
           KETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI
Sbjct: 241 KETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI 351
           DGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI
Sbjct: 301 DGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI 350

[Close]

BLAST of Carg02443 vs. ExPASy TrEMBL
Match: A0A6J1K321 (L-lactate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111491257 PE=3 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 7.3e-187
Identity = 343/350 (98.00%), Postives = 346/350 (98.86%), Query Frame = 0


Query: 1   MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD 60
           MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LADELVLVDAKPNKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L DELVLVDAKP+KLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG
Sbjct: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQYSPDT LLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQYSPDTKLLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLK+QQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240

Query: 241 KETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI 300
           KETLENIHKEVI GAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI
Sbjct: 241 KETLENIHKEVIEGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI 351
           DG DLFLSLPARLGRGGV+GITNVHLTTEEMRRLQDSANTILQVQSELGI
Sbjct: 301 DGDDLFLSLPARLGRGGVMGITNVHLTTEEMRRLQDSANTILQVQSELGI 350

[Close]

BLAST of Carg02443 vs. ExPASy TrEMBL
Match: A0A5A7T9X6 (L-lactate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00390 PE=3 SV=1)

HSP 1 Score: 639.0 bits (1647), Expect = 1.1e-179
Identity = 329/350 (94.00%), Postives = 341/350 (97.43%), Query Frame = 0


Query: 1   MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD 60
           MQKSPSAS+LGPGGLDLSQAFFKPI DASPPS S  NTKISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASSLGPGGLDLSQAFFKPIRDASPPSISNRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LADELVLVDAKPNKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L DELVLVDAKP+KLRGEMLDLQHAAAFLPRTKISASTDYSITAGS+LCIVTAGARQIAG
Sbjct: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSNLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQ+SP+TILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLK+QQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240

Query: 241 KETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI 300
           KETLE IHKEVIGGAYEVI LKGYTSWAIGYSVASLARS+LRDQ+RIHPVSVLAKGFYGI
Sbjct: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI 351
           DGGDLFLSLPA+LGRGGVLGITNVHLT EE +RL+DSANTILQVQ+ELGI
Sbjct: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 350

[Close]

BLAST of Carg02443 vs. ExPASy TrEMBL
Match: A0A1S3ATB9 (L-lactate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103482619 PE=3 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 2.5e-179
Identity = 328/350 (93.71%), Postives = 340/350 (97.14%), Query Frame = 0


Query: 1   MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD 60
           MQKSPS S+LGPGGLDLSQAFFKPI DASPPSTS  NTKISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSPSTSSLGPGGLDLSQAFFKPIRDASPPSTSNRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LADELVLVDAKPNKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L DELVLVDAKP+KLRGEMLDLQHAAAFLPRTKISASTDYS TAGS+LCIVTAGARQIAG
Sbjct: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSTTAGSNLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQ+SP+TILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLK+QQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240

Query: 241 KETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI 300
           KETLE IHKEVIGGAYEVI LKGYTSWAIGYSVASLARS+LRDQ+RIHPVSVLAKGFYGI
Sbjct: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI 351
           DGGDLFLSLPA+LGRGGVLGITNVHLT EE +RL+DSANTILQVQ+ELGI
Sbjct: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 350

[Close]

BLAST of Carg02443 vs. ExPASy TrEMBL
Match: A0A6J1E3A1 (L-lactate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111430410 PE=3 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 2.4e-177
Identity = 324/350 (92.57%), Postives = 341/350 (97.43%), Query Frame = 0


Query: 1   MQKSPSASALGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTILTQD 60
           M+KS SAS+LGPGGLD+SQAFFKP+ DASPPSTSK NTKISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MEKSSSASSLGPGGLDISQAFFKPVSDASPPSTSKRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LADELVLVDAKPNKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LADELVLVDAKP+KLRGEMLDLQHAAAFLPRTKISASTDYS+TA SDLCIVTAGARQIAG
Sbjct: 61  LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQYSP+TILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFL++QQI YE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQITYE 240

Query: 241 KETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGFYGI 300
           KETLE+IHKEVIGGAYEVI LKGYTSWAIGYSVASLARSLLRDQ+RIHPVSVLAKGFYGI
Sbjct: 241 KETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI 351
           +GG+LFLSLPA+LGRGGVLGITNVHLT EE  RL+DSANTILQVQ++LGI
Sbjct: 301 NGGELFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI 350

[Close]

BLAST of Carg02443 vs. TAIR 10
Match: AT4G17260.1 (Lactate/malate dehydrogenase family protein )

HSP 1 Score: 546.6 bits (1407), Expect = 1.5e-155
Identity = 272/353 (77.05%), Postives = 315/353 (89.24%), Query Frame = 0


Query: 1   MQKSPSASA---LGPGGLDLSQAFFKPIPDASPPSTSKGNTKISVIGAGNVGMAIAQTIL 60
           M+K+ S S+   LGP GLDL+ AFFKPI ++ P   S   TK+SV+G GNVGMAIAQTIL
Sbjct: 1   MEKNASTSSLKDLGPSGLDLTSAFFKPIHNSDPSLPSNRRTKVSVVGVGNVGMAIAQTIL 60

Query: 61  TQDLADELVLVDAKPNKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQ 120
           TQDLADE+ LVDAKP+KLRGEMLDLQHAAAFLPRTKI+AS DY +TAGSDLCIVTAGARQ
Sbjct: 61  TQDLADEIALVDAKPDKLRGEMLDLQHAAAFLPRTKITASVDYEVTAGSDLCIVTAGARQ 120

Query: 121 IAGESRLNLLQRNVALFQKIVPPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIG 180
             GESRLNLLQRNVALF+ I+PPL + SPD+IL+IVSNPVDVLTYVAWKLSGFP NRV+G
Sbjct: 121 NPGESRLNLLQRNVALFRHIIPPLAKASPDSILIIVSNPVDVLTYVAWKLSGFPVNRVLG 180

Query: 181 SGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKSQQI 240
           SGTNLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGG+P+LSFL+  QI
Sbjct: 181 SGTNLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGIPVLSFLEKNQI 240

Query: 241 AYEKETLENIHKEVIGGAYEVIRLKGYTSWAIGYSVASLARSLLRDQQRIHPVSVLAKGF 300
           AYEK+TLE+IH+ V+G AYEVI LKGYTSWAIGYSVA+LAR++LRDQ++IHPV+VLA+GF
Sbjct: 241 AYEKQTLEDIHQAVVGSAYEVIGLKGYTSWAIGYSVANLARTILRDQRKIHPVTVLARGF 300

Query: 301 YGIDGGDLFLSLPARLGRGGVLGITNVHLTTEEMRRLQDSANTILQVQSELGI 351
           YG+DGGD+FLSLPA LGR GV+ +TNVH+T EE  +LQ SA TIL++QS+LG+
Sbjct: 301 YGVDGGDVFLSLPALLGRNGVVAVTNVHMTDEEAEKLQKSAKTILEMQSQLGL 353

The following BLAST results are available for this feature:

BLAST of Carg02443 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7036127.1	1.0e-190	100.00	hypothetical protein SDJN02_02927, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023534013.1	1.1e-189	99.14	L-lactate dehydrogenase A-like [Cucurbita pepo subsp. pepo]	[more]
XP_022958393.1	1.1e-189	99.43	L-lactate dehydrogenase A-like [Cucurbita moschata]	[more]
KAG6606181.1	4.2e-189	99.14	hypothetical protein SDJN03_03498, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022995851.1	1.5e-186	98.00	L-lactate dehydrogenase A-like [Cucurbita maxima]	[more]

BLAST of Carg02443 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P22988	5.7e-128	69.25	L-lactate dehydrogenase A OS=Hordeum vulgare OX=4513 PE=1 SV=1	[more]
P22989	1.5e-125	67.54	L-lactate dehydrogenase B (Fragment) OS=Hordeum vulgare OX=4513 PE=1 SV=1	[more]
P29038	3.9e-121	66.20	L-lactate dehydrogenase OS=Zea mays OX=4577 PE=2 SV=1	[more]
Q9XT87	4.1e-94	55.13	L-lactate dehydrogenase A chain OS=Monodelphis domestica OX=13616 GN=LDHA PE=2 S...	[more]
P06151	9.1e-94	56.13	L-lactate dehydrogenase A chain OS=Mus musculus OX=10090 GN=Ldha PE=1 SV=3	[more]

BLAST of Carg02443 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H2Z1	5.4e-190	99.43	L-lactate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111459629 PE=3 SV=1	[more]
A0A6J1K321	7.3e-187	98.00	L-lactate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111491257 PE=3 SV=1	[more]
A0A5A7T9X6	1.1e-179	94.00	L-lactate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A1S3ATB9	2.5e-179	93.71	L-lactate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103482619 PE=3 SV=1	[more]
A0A6J1E3A1	2.4e-177	92.57	L-lactate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111430410 PE=3 SV=1	[more]

BLAST of Carg02443 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G17260.1	1.5e-155	77.05	Lactate/malate dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001557	L-lactate/malate dehydrogenase	PRINTS	PR00086	LLDHDRGNASE	coord: 39..63 score: 50.38 coord: 205..218 score: 67.82 coord: 175..193 score: 68.42 coord: 64..88 score: 49.94 coord: 151..171 score: 71.81
IPR001557	L-lactate/malate dehydrogenase	PIRSF	PIRSF000102	Lac_mal_DH	coord: 34..350 e-value: 7.8E-103 score: 341.8
None	No IPR available	GENE3D	3.40.50.720		coord: 35..179 e-value: 9.7E-59 score: 199.5
None	No IPR available	PANTHER	PTHR43128	L-2-HYDROXYCARBOXYLATE DEHYDROGENASE (NAD(P)(+))	coord: 1..350
None	No IPR available	PANTHER	PTHR43128:SF23	L-LACTATE DEHYDROGENASE	coord: 1..350
None	No IPR available	CDD	cd05293	LDH_1	coord: 35..347 e-value: 8.66051E-179 score: 495.971
IPR022383	Lactate/malate dehydrogenase, C-terminal	PFAM	PF02866	Ldh_1_C	coord: 180..342 e-value: 4.1E-24 score: 85.5
IPR011304	L-lactate dehydrogenase	TIGRFAM	TIGR01771	TIGR01771	coord: 42..341 e-value: 2.6E-115 score: 382.8
IPR011304	L-lactate dehydrogenase	HAMAP	MF_00488	Lactate_dehydrog	coord: 37..349 score: 37.389771
IPR001236	Lactate/malate dehydrogenase, N-terminal	PFAM	PF00056	Ldh_1_N	coord: 38..177 e-value: 2.2E-44 score: 150.9
IPR015955	Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal	GENE3D	3.90.110.10		coord: 180..348 e-value: 2.0E-58 score: 198.6
IPR015955	Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal	SUPERFAMILY	56327	LDH C-terminal domain-like	coord: 178..347
IPR018177	L-lactate dehydrogenase, active site	PROSITE	PS00064	L_LDH	coord: 207..213
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 25..177

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg02443-RA	Carg02443-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0019752	carboxylic acid metabolic process
biological_process	GO:0009737	response to abscisic acid
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004459	L-lactate dehydrogenase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor