Carg02396 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg02396 vs. NCBI nr
Match: KAG7016280.1 (RNA pseudouridine synthase 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 894.8 bits (2311), Expect = 2.9e-256
Identity = 433/433 (100.00%), Postives = 433/433 (100.00%), Query Frame = 0

Query: 1   MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLIEFYSRKYKSSAPLEGLLLIINETY 60
           MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLIEFYSRKYKSSAPLEGLLLIINETY
Sbjct: 1   MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLIEFYSRKYKSSAPLEGLLLIINETY 60

Query: 61  FTSITLQLVAANSERTDNDRWSGYHRSRNNSQVLHPGSFTPDLMVCSKVCITALGVFIYY 120
           FTSITLQLVAANSERTDNDRWSGYHRSRNNSQVLHPGSFTPDLMVCSKVCITALGVFIYY
Sbjct: 61  FTSITLQLVAANSERTDNDRWSGYHRSRNNSQVLHPGSFTPDLMVCSKVCITALGVFIYY 120

Query: 121 KICRIGSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL 180
           KICRIGSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL
Sbjct: 121 KICRIGSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL 180

Query: 181 QWWSSKQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNN 240
           QWWSSKQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNN
Sbjct: 181 QWWSSKQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNN 240

Query: 241 TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVN 300
           TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVN
Sbjct: 241 TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVN 300

Query: 301 VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLMGDPLYVAGGQPNCLHSELVDQSF 360
           VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLMGDPLYVAGGQPNCLHSELVDQSF
Sbjct: 301 VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLMGDPLYVAGGQPNCLHSELVDQSF 360

Query: 361 AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKIVAPLPPILRTHGESEDAGNRL 420
           AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKIVAPLPPILRTHGESEDAGNRL
Sbjct: 361 AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKIVAPLPPILRTHGESEDAGNRL 420

Query: 421 GINKPLHGGCQKD 434
           GINKPLHGGCQKD
Sbjct: 421 GINKPLHGGCQKD 433

BLAST of Carg02396 vs. NCBI nr
Match: KAG6578753.1 (RNA pseudouridine synthase 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 729.2 bits (1881), Expect = 2.1e-206
Identity = 368/433 (84.99%), Postives = 369/433 (85.22%), Query Frame = 0

Query: 1   MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLIEFYSRKYKSSAPLEGLLLIINETY 60
           MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLIEFYSRKYKSSAPLEG L  I    
Sbjct: 1   MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLIEFYSRKYKSSAPLEGWLQRI---- 60

Query: 61  FTSITLQLVAANSERTDNDRWSGYHRSRNNSQVLHPGSFTPDLMVCSKVCITALGVFIYY 120
                                        N Q+   G    D                  
Sbjct: 61  ----------------------------QNEQITIDGQVITDPET--------------- 120

Query: 121 KICRIGSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL 180
            I RIGSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL
Sbjct: 121 -ILRIGSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL 180

Query: 181 QWWSSKQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNN 240
           QWWSSKQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNN
Sbjct: 181 QWWSSKQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNN 240

Query: 241 TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVN 300
           TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVN
Sbjct: 241 TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVN 300

Query: 301 VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLMGDPLYVAGGQPNCLHSELVDQSF 360
           VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLMGDPLYVAGGQPNCLHSELVDQSF
Sbjct: 301 VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLMGDPLYVAGGQPNCLHSELVDQSF 360

Query: 361 AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKIVAPLPPILRTHGESEDAGNRL 420
           AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKIVAPLPPILRTHGESEDAGNRL
Sbjct: 361 AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKIVAPLPPILRTHGESEDAGNRL 385

Query: 421 GINKPLHGGCQKD 434
           GINKPLHGGCQKD
Sbjct: 421 GINKPLHGGCQKD 385

BLAST of Carg02396 vs. NCBI nr
Match: XP_022938426.1 (RNA pseudouridine synthase 5 isoform X1 [Cucurbita moschata] >XP_022938427.1 RNA pseudouridine synthase 5 isoform X1 [Cucurbita moschata])

HSP 1 Score: 696.8 bits (1797), Expect = 1.2e-196
Identity = 352/421 (83.61%), Postives = 357/421 (84.80%), Query Frame = 0

Query: 1   MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLIEFYSRKYKSSAPLEGLLLIINETY 60
           MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLIEFYSRKYKSSAPLEG L  I    
Sbjct: 1   MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLIEFYSRKYKSSAPLEGWLQRI---- 60

Query: 61  FTSITLQLVAANSERTDNDRWSGYHRSRNNSQVLHPGSFTPDLMVCSKVCITALGVFIYY 120
                                        N Q+   G    D                  
Sbjct: 61  ----------------------------QNEQITIDGQVITDPET--------------- 120

Query: 121 KICRIGSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL 180
            I RIGSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL
Sbjct: 121 -ILRIGSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL 180

Query: 181 QWWSSKQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNN 240
           QWWSSKQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQF+AYFADGTSCVGRNRNN
Sbjct: 181 QWWSSKQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQFAAYFADGTSCVGRNRNN 240

Query: 241 TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVN 300
           TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVN
Sbjct: 241 TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVN 300

Query: 301 VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLMGDPLYVAGGQPNCLHSELVDQSF 360
           VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPL+GDPLYVAGGQPNCLHSELVDQSF
Sbjct: 301 VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLIGDPLYVAGGQPNCLHSELVDQSF 360

Query: 361 AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKIVAPLPPILRTHGESEDAGNRL 420
           AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKI+APLPPILRTHGESEDAGNR+
Sbjct: 361 AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKILAPLPPILRTHGESEDAGNRV 373

Query: 421 G 422
           G
Sbjct: 421 G 373

BLAST of Carg02396 vs. NCBI nr
Match: XP_022994066.1 (RNA pseudouridine synthase 5 [Cucurbita maxima])

HSP 1 Score: 690.3 bits (1780), Expect = 1.1e-194
Identity = 349/421 (82.90%), Postives = 355/421 (84.32%), Query Frame = 0

Query: 1   MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLIEFYSRKYKSSAPLEGLLLIINETY 60
           MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLI+FYSRKYKSSAPLEG L  I    
Sbjct: 1   MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLIDFYSRKYKSSAPLEGWLQRI---- 60

Query: 61  FTSITLQLVAANSERTDNDRWSGYHRSRNNSQVLHPGSFTPDLMVCSKVCITALGVFIYY 120
                                        N Q+   G    D                  
Sbjct: 61  ----------------------------QNEQITIDGQVITDPET--------------- 120

Query: 121 KICRIGSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL 180
            I RIGSEVVYRR PWKEPDTP LLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL
Sbjct: 121 -ILRIGSEVVYRRFPWKEPDTPCLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL 180

Query: 181 QWWSSKQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNN 240
           QWWSSKQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQF+AYFADGTSCVGR+RNN
Sbjct: 181 QWWSSKQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQFAAYFADGTSCVGRSRNN 240

Query: 241 TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVN 300
           TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVN
Sbjct: 241 TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVN 300

Query: 301 VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLMGDPLYVAGGQPNCLHSELVDQSF 360
           VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPL+GDPLYVAGGQPNCLHSELVDQSF
Sbjct: 301 VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLLGDPLYVAGGQPNCLHSELVDQSF 360

Query: 361 AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKIVAPLPPILRTHGESEDAGNRL 420
           AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKIVAPLPPILRTHGESEDAGNR+
Sbjct: 361 AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKIVAPLPPILRTHGESEDAGNRV 373

Query: 421 G 422
           G
Sbjct: 421 G 373

BLAST of Carg02396 vs. NCBI nr
Match: XP_023551339.1 (RNA pseudouridine synthase 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 688.7 bits (1776), Expect = 3.2e-194
Identity = 348/421 (82.66%), Postives = 355/421 (84.32%), Query Frame = 0

Query: 1   MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLIEFYSRKYKSSAPLEGLLLIINETY 60
           MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLIEFYSRKYKSSAPLEG L  I    
Sbjct: 1   MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLIEFYSRKYKSSAPLEGWLQRI---- 60

Query: 61  FTSITLQLVAANSERTDNDRWSGYHRSRNNSQVLHPGSFTPDLMVCSKVCITALGVFIYY 120
                                        N Q+   G    D                  
Sbjct: 61  ----------------------------QNEQITIDGQVITDPET--------------- 120

Query: 121 KICRIGSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL 180
            I RIGSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL
Sbjct: 121 -ILRIGSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL 180

Query: 181 QWWSSKQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNN 240
           QWWSSKQSSSVS+ELHPVPVHRLGRGTSGILLCAKTRLARTQF+AYFADGTSCVGRNRNN
Sbjct: 181 QWWSSKQSSSVSKELHPVPVHRLGRGTSGILLCAKTRLARTQFAAYFADGTSCVGRNRNN 240

Query: 241 TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVN 300
           TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPA+SKVN
Sbjct: 241 TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAFSKVN 300

Query: 301 VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLMGDPLYVAGGQPNCLHSELVDQSF 360
           VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPL+GDPLYVAGGQPNCLHS LVDQSF
Sbjct: 301 VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLIGDPLYVAGGQPNCLHSGLVDQSF 360

Query: 361 AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKIVAPLPPILRTHGESEDAGNRL 420
           AEDGGY+RP KPVPGDCGYYLHAHQLVLHHPKTSEVVKIVAPLPPILRTHGESEDAGNR+
Sbjct: 361 AEDGGYRRPAKPVPGDCGYYLHAHQLVLHHPKTSEVVKIVAPLPPILRTHGESEDAGNRV 373

Query: 421 G 422
           G
Sbjct: 421 G 373

BLAST of Carg02396 vs. ExPASy Swiss-Prot
Match: Q5M721 (RNA pseudouridine synthase 5 OS=Arabidopsis thaliana OX=3702 GN=At3g52260 PE=2 SV=2)

HSP 1 Score: 443.7 bits (1140), Expect = 2.3e-123
Identity = 239/413 (57.87%), Postives = 283/413 (68.52%), Query Frame = 0

Query: 7   QVIGIPWPDLNDGLFYNDVVKPSDSGMSLI-EFYSRKYKSSAPLEGLLLIINETYFTSIT 66
           Q IG+PWP+LNDGL Y D +  S+S ++ + EFY  KYKSSAPL G +  I         
Sbjct: 6   QRIGLPWPELNDGLAYKDAISSSESELTTVSEFYFTKYKSSAPLLGWIQRI--------- 65

Query: 67  LQLVAANSERTDNDRWSGYHRSRNNSQVLHPGSFTPDLMVCSKVCITALGVFIYYKICRI 126
                                   N Q+   G    D                   + R 
Sbjct: 66  -----------------------QNGQIQIDGEVVKDPNT----------------LLRS 125

Query: 127 GSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQLQWWSS 186
           GS++VY RLPWKEPDTPY LEVLYEDD++IALNKPSGLQVLPGG+FQQRTVLTQLQW   
Sbjct: 126 GSKLVYSRLPWKEPDTPYSLEVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFG 185

Query: 187 KQSSSVS--EELHPVPVHRLGRGTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNNTME 246
           K  S +   E  HPVPVHRLGRGTSGILLCAKT+LA+T+ +AYFA+GTS VG + N   E
Sbjct: 186 KNDSYIGSRESPHPVPVHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVG-SGNLDQE 245

Query: 247 EGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVNVLE 306
            GT RK+SKIYRAL  GI+ +DEV I QPIG +RYPGVAQGLYVA+  GKPA+SKV VLE
Sbjct: 246 CGTGRKLSKIYRALADGIVEEDEVVIKQPIGVVRYPGVAQGLYVASPEGKPAFSKVFVLE 305

Query: 307 RDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLMGDPLYVAGGQPNCLHSELVDQ--SFA 366
           RD +   +LV+VEIQSGRPHQIRIHL+ +GHPL+GDPLYVAGGQP C   +LVD   +FA
Sbjct: 306 RDREKNCSLVKVEIQSGRPHQIRIHLAYMGHPLVGDPLYVAGGQPKCFDPDLVDDAAAFA 365

Query: 367 EDGGYQRPTKPVPGDCGYYLHAHQLVLHH-PKTSEVVKIVAPLPPILRTHGES 414
           EDGGY+RP + VPGDCGY+LHAHQ+ L +   T +VVKIVAPLPPIL+T  E+
Sbjct: 366 EDGGYRRPNQAVPGDCGYHLHAHQVELPNLLNTHKVVKIVAPLPPILQTRCET 369

BLAST of Carg02396 vs. ExPASy Swiss-Prot
Match: Q0DST9 (RNA pseudouridine synthase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0288500 PE=2 SV=2)

HSP 1 Score: 411.0 bits (1055), Expect = 1.7e-113
Identity = 226/421 (53.68%), Postives = 271/421 (64.37%), Query Frame = 0

Query: 10  GIPWPDLNDGLFYNDVVKPSDSG--MSLIEFYSRKYKSSAPLEGLLLIINETYFTSITLQ 69
           G PWP+ N+G+ Y D  + +D+G   +LIEFYS  YKSSAPL G +  I +        Q
Sbjct: 25  GTPWPEFNEGISYIDTFRCADAGATTTLIEFYSTSYKSSAPLPGWIKRIRDG-------Q 84

Query: 70  LVAANSERTDNDRWSGYHRSRNNSQVLHPGSFTPDLMVCSKVCITALGVFIYYKICRIGS 129
           +       TD                       PD+                  I R GS
Sbjct: 85  ITVDGEVATD-----------------------PDM------------------ILREGS 144

Query: 130 EVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQLQWWSSKQ 189
           ++VY RLPW+EP  P+LL+VLYEDD+MIALNKPSGLQVLP G+FQQRTVL QLQ    K 
Sbjct: 145 KLVYHRLPWQEPFAPHLLDVLYEDDDMIALNKPSGLQVLPKGLFQQRTVLAQLQLKDWKM 204

Query: 190 SSSV-----SEELHPVPVHRLGRGTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNNTM 249
            SS        + HPVPVHRLGRGTSG+LLCAKT+LA+ Q +AYFA+G +  G++R+ T 
Sbjct: 205 PSSFCSKRKDAQSHPVPVHRLGRGTSGLLLCAKTKLAKAQLAAYFAEGATNAGKSRDET- 264

Query: 250 EEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVNVL 309
           +    RKISK YRALV GI+ +DEV I QPIG +RYPGVA+GLY A SSGKPA SKV VL
Sbjct: 265 DICKARKISKFYRALVTGILENDEVMITQPIGLVRYPGVAEGLYAACSSGKPAMSKVRVL 324

Query: 310 ERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLMGDPLYVAGGQPNCLHSELVD--QSF 369
           ER +   HTL+QVEI SGRPHQIRIHL+ IGHPL+ DPLY  GGQP     E      SF
Sbjct: 325 ERLKIHNHTLIQVEIHSGRPHQIRIHLAYIGHPLVDDPLYGIGGQPKFDEPEPTSTADSF 384

Query: 370 AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKIVAPLPPILRTHGESEDAGNRL 422
           A DGGY+RP +PVPGDCGY+LHAH LVL HP T+E++KI APLP IL+T  E +D    L
Sbjct: 385 AYDGGYERPLQPVPGDCGYHLHAHWLVLCHPTTNEMIKITAPLPHILQTREEQQDTAKLL 396

BLAST of Carg02396 vs. ExPASy Swiss-Prot
Match: P72970 (Uncharacterized RNA pseudouridine synthase slr1592 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr1592 PE=3 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 8.2e-44
Identity = 109/285 (38.25%), Postives = 151/285 (52.98%), Query Frame = 0

Query: 126 GSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQLQWWSS 185
           G  + Y R PW EP+ P  L VL+ D+++ A++KPSGL VLPGG F   TVL QL+    
Sbjct: 42  GQTLTYWRSPWVEPEVPLQLSVLHRDEDLWAIDKPSGLPVLPGGDFVYHTVLEQLKIQYP 101

Query: 186 KQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNNTMEEG 245
           ++S          P+HRLG GTSG+LL  ++ L R + S  F +                
Sbjct: 102 QESL--------FPLHRLGTGTSGLLLLGRSTLGRRELSRQFRE---------------- 161

Query: 246 TKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVNVLERD 305
                 KIYR ++      ++    Q IG++ YP +   +Y A+S GKPA S   +L R 
Sbjct: 162 --HICRKIYRTVIGPCDLPEKFECHQAIGKIPYPQLGY-IYAASSHGKPAQSYGRILARS 221

Query: 306 EQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLMGDPLYVAGGQP-NCLHSELVDQSFAEDG 365
            +   T ++VEI++GRPHQIRIHL+S+G+PL+GD LY  GG P NC  +           
Sbjct: 222 PE--KTWLEVEIRTGRPHQIRIHLASLGYPLLGDRLYGPGGVPINCRTAR---------- 278

Query: 366 GYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKIVAPLPPILRT 410
                    P D GY LH++QL   H  T + +K+ APLPP L T
Sbjct: 282 ---------PSDGGYTLHSYQLGFLHLHTQQAIKLTAPLPPELIT 278

BLAST of Carg02396 vs. ExPASy Swiss-Prot
Match: P33640 (Ribosomal large subunit pseudouridine synthase D OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=rluD PE=3 SV=2)

HSP 1 Score: 120.6 bits (301), Expect = 4.5e-26
Identity = 86/260 (33.08%), Postives = 129/260 (49.62%), Query Frame = 0

Query: 145 LEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQLQWWSSKQSSSVSEELHPVPVHRLG 204
           LE++YED+ ++ ++KP+GL V P    Q  T+L  L +        ++       VHRL 
Sbjct: 83  LEIVYEDEHILVIDKPAGLVVHPAAGHQDGTLLNALLY----HVPDIANVPRAGIVHRLD 142

Query: 205 RGTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNNTMEEGTKRKISKIYRALVAGIIHD 264
           + T+G+++ AKT  A T+  A                      R +S+IY A+V G+I  
Sbjct: 143 KDTTGLMVVAKTLEAHTKLVAQL------------------QARSVSRIYEAIVIGVITS 202

Query: 265 DEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVNVLERDEQGKHTLVQVEIQSGRPHQ 324
              +ID PIGR    GV +       +GK A S   VLER     HT  +V++++GR HQ
Sbjct: 203 GG-TIDAPIGR---HGVQRQKMAVVDAGKVAVSHYRVLERFR--AHTHTRVKLETGRTHQ 262

Query: 325 IRIHLSSIGHPLMGDPLYVAGGQPNCLHSELVDQSFAEDGGYQRPTKPVPGDCGYYLHAH 384
           IR+H+S IG+PL+GDP+Y    +   + S+ + Q+  E   + R            LHA 
Sbjct: 263 IRVHMSHIGYPLVGDPVYGGRFRIPPVASQTLVQTLRE---FPRQA----------LHAR 301

Query: 385 QLVLHHPKTSEVVKIVAPLP 405
            L L HP T   +K  +PLP
Sbjct: 323 FLELDHPATGVRMKWESPLP 301

BLAST of Carg02396 vs. ExPASy Swiss-Prot
Match: P74346 (Uncharacterized RNA pseudouridine synthase slr1629 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr1629 PE=3 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 1.0e-25
Identity = 82/262 (31.30%), Postives = 129/262 (49.24%), Query Frame = 0

Query: 145 LEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQLQWWSSKQSSSVSEELHPVPVHRLG 204
           L++LYED+++I +NKP+GL V PG      TV+  L        + +     P  VHRL 
Sbjct: 98  LDILYEDEQLIIINKPAGLVVHPGPGHPDGTVVNAL-LAHCPDLAGIGGVQRPGIVHRLD 157

Query: 205 RGTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNNTMEEGTKRKISKIYRALVAGIIHD 264
           + T+G ++ AKT LA                 N    ++E T R   ++Y  +V G   +
Sbjct: 158 KDTTGAMVVAKTELAL---------------HNLQVQLKEKTAR---RLYWGIVYGSPKE 217

Query: 265 DEVSIDQPIGRMRYPGVAQ--GLYVATSSGKPAYSKVNVLERDEQGKHTLVQVEIQSGRP 324
            + +++ P+G  R+PG  Q  G+      G+ A +   +LER   G H+ ++ ++++GR 
Sbjct: 218 IQGTVNLPVG--RHPGDRQKMGIVPVEKGGREAVTHWRLLER--IGNHSWLEFQLETGRT 277

Query: 325 HQIRIHLSSIGHPLMGDPLYVAGGQPNCLHSELVDQSFAEDGGYQRPTKPVPGDCGYYLH 384
           HQIR+H   +GHPL+GD LY + G  N                       +PG     LH
Sbjct: 278 HQIRVHSKQMGHPLVGDNLYTSPGSVNV---------------------NLPGQA---LH 312

Query: 385 AHQLVLHHPKTSEVVKIVAPLP 405
           AHQL L HP + E++  +AP+P
Sbjct: 338 AHQLSLIHPVSGEIITAIAPMP 312

BLAST of Carg02396 vs. ExPASy TrEMBL
Match: A0A6J1FE06 (RNA pseudouridine synthase 5 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444674 PE=4 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 5.6e-197
Identity = 352/421 (83.61%), Postives = 357/421 (84.80%), Query Frame = 0

Query: 1   MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLIEFYSRKYKSSAPLEGLLLIINETY 60
           MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLIEFYSRKYKSSAPLEG L  I    
Sbjct: 1   MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLIEFYSRKYKSSAPLEGWLQRI---- 60

Query: 61  FTSITLQLVAANSERTDNDRWSGYHRSRNNSQVLHPGSFTPDLMVCSKVCITALGVFIYY 120
                                        N Q+   G    D                  
Sbjct: 61  ----------------------------QNEQITIDGQVITDPET--------------- 120

Query: 121 KICRIGSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL 180
            I RIGSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL
Sbjct: 121 -ILRIGSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL 180

Query: 181 QWWSSKQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNN 240
           QWWSSKQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQF+AYFADGTSCVGRNRNN
Sbjct: 181 QWWSSKQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQFAAYFADGTSCVGRNRNN 240

Query: 241 TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVN 300
           TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVN
Sbjct: 241 TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVN 300

Query: 301 VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLMGDPLYVAGGQPNCLHSELVDQSF 360
           VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPL+GDPLYVAGGQPNCLHSELVDQSF
Sbjct: 301 VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLIGDPLYVAGGQPNCLHSELVDQSF 360

Query: 361 AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKIVAPLPPILRTHGESEDAGNRL 420
           AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKI+APLPPILRTHGESEDAGNR+
Sbjct: 361 AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKILAPLPPILRTHGESEDAGNRV 373

Query: 421 G 422
           G
Sbjct: 421 G 373

BLAST of Carg02396 vs. ExPASy TrEMBL
Match: A0A6J1JY31 (RNA pseudouridine synthase 5 OS=Cucurbita maxima OX=3661 GN=LOC111489868 PE=4 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 5.3e-195
Identity = 349/421 (82.90%), Postives = 355/421 (84.32%), Query Frame = 0

Query: 1   MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLIEFYSRKYKSSAPLEGLLLIINETY 60
           MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLI+FYSRKYKSSAPLEG L  I    
Sbjct: 1   MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLIDFYSRKYKSSAPLEGWLQRI---- 60

Query: 61  FTSITLQLVAANSERTDNDRWSGYHRSRNNSQVLHPGSFTPDLMVCSKVCITALGVFIYY 120
                                        N Q+   G    D                  
Sbjct: 61  ----------------------------QNEQITIDGQVITDPET--------------- 120

Query: 121 KICRIGSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL 180
            I RIGSEVVYRR PWKEPDTP LLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL
Sbjct: 121 -ILRIGSEVVYRRFPWKEPDTPCLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL 180

Query: 181 QWWSSKQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNN 240
           QWWSSKQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQF+AYFADGTSCVGR+RNN
Sbjct: 181 QWWSSKQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQFAAYFADGTSCVGRSRNN 240

Query: 241 TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVN 300
           TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVN
Sbjct: 241 TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVN 300

Query: 301 VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLMGDPLYVAGGQPNCLHSELVDQSF 360
           VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPL+GDPLYVAGGQPNCLHSELVDQSF
Sbjct: 301 VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLLGDPLYVAGGQPNCLHSELVDQSF 360

Query: 361 AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKIVAPLPPILRTHGESEDAGNRL 420
           AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKIVAPLPPILRTHGESEDAGNR+
Sbjct: 361 AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKIVAPLPPILRTHGESEDAGNRV 373

Query: 421 G 422
           G
Sbjct: 421 G 373

BLAST of Carg02396 vs. ExPASy TrEMBL
Match: A0A6J1BWD7 (RNA pseudouridine synthase 5 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111006297 PE=4 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 4.7e-175
Identity = 312/415 (75.18%), Postives = 332/415 (80.00%), Query Frame = 0

Query: 1   MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLIEFYSRKYKSSAPLEGLLLIINETY 60
           MLG S  VIG+PWPDLNDGLFYNDVVKPSDSG +LIEFYS KYKSSAPLEG L  I    
Sbjct: 1   MLGTSSPVIGVPWPDLNDGLFYNDVVKPSDSGKALIEFYSSKYKSSAPLEGWLQRI---- 60

Query: 61  FTSITLQLVAANSERTDNDRWSGYHRSRNNSQVLHPGSFTPDLMVCSKVCITALGVFIYY 120
                                        N Q+   G    D                  
Sbjct: 61  ----------------------------QNEQITIDGQIITDPET--------------- 120

Query: 121 KICRIGSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL 180
            I RIGSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRT+LTQL
Sbjct: 121 -ILRIGSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTILTQL 180

Query: 181 QWWSSKQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNN 240
           QWWS KQSSSVSE  HPVPVHRLGRGTSGILLCAKTRLARTQ +AYFADG  C+GRNRN 
Sbjct: 181 QWWSRKQSSSVSEGSHPVPVHRLGRGTSGILLCAKTRLARTQLAAYFADGAFCIGRNRNT 240

Query: 241 TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVN 300
           +M EGTKRKISKIYRALV GI+HDDEVSIDQPIGR+ YPGVAQGLYVATSSGKPA+SKV+
Sbjct: 241 SMVEGTKRKISKIYRALVTGIVHDDEVSIDQPIGRIHYPGVAQGLYVATSSGKPAFSKVD 300

Query: 301 VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLMGDPLYVAGGQPNCLHSELVDQSF 360
           VLERDEQG +TLVQVEIQSGRPHQIRIHLSSIGHPL+GDPLYVAGGQPNCLHS+ V+QSF
Sbjct: 301 VLERDEQGNNTLVQVEIQSGRPHQIRIHLSSIGHPLLGDPLYVAGGQPNCLHSDSVNQSF 360

Query: 361 AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKIVAPLPPILRTHGESED 416
           AEDGGYQRPTKPVPGDCGYYLHAHQLVL HP+T+E VKI+APLPPILRTHGE+E+
Sbjct: 361 AEDGGYQRPTKPVPGDCGYYLHAHQLVLDHPRTNEAVKIIAPLPPILRTHGEAEE 367

BLAST of Carg02396 vs. ExPASy TrEMBL
Match: A0A1S3CPT2 (RNA pseudouridine synthase 5 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103503310 PE=4 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 2.6e-173
Identity = 314/418 (75.12%), Postives = 334/418 (79.90%), Query Frame = 0

Query: 1   MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLIEFYSRKYKSSAPLEGLLLIINETY 60
           +LGRSFQVIGIPWPDLNDGLFYNDVVKP DSG SLI FYS KYKSSAPLEG L  I    
Sbjct: 2   LLGRSFQVIGIPWPDLNDGLFYNDVVKPPDSGKSLIHFYSCKYKSSAPLEGWLQRI---- 61

Query: 61  FTSITLQLVAANSERTDNDRWSGYHRSRNNSQVLHPGSFTPDLMVCSKVCITALGVFIYY 120
                                        N Q+   G    D                  
Sbjct: 62  ----------------------------KNEQITIDGEVITDPET--------------- 121

Query: 121 KICRIGSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL 180
            I RIGSE+VYRRLPWKEPDTP+ LE+LYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL
Sbjct: 122 -ILRIGSELVYRRLPWKEPDTPFSLEILYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL 181

Query: 181 QWWSSKQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNN 240
           QWWSS QS S+SEELHPVPVHRLGRGTSGILLCAKT+LARTQF+AYFADGTS V   RN+
Sbjct: 182 QWWSSDQSFSISEELHPVPVHRLGRGTSGILLCAKTKLARTQFAAYFADGTSSV--IRNS 241

Query: 241 TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVN 300
           T EEGTKRKISKIYRALV GIIHDDEVS+DQPIGRM+YPGVAQGLYVAT SGKPA SKVN
Sbjct: 242 TSEEGTKRKISKIYRALVTGIIHDDEVSVDQPIGRMQYPGVAQGLYVATPSGKPACSKVN 301

Query: 301 VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLMGDPLYVAGGQPNCLHSELVDQSF 360
           VLERDEQG+HTLVQVEIQSGRPHQIRIHLSSIGHPL+GDPLYVAGGQPNCLHSE+V+QSF
Sbjct: 302 VLERDEQGEHTLVQVEIQSGRPHQIRIHLSSIGHPLLGDPLYVAGGQPNCLHSEVVNQSF 361

Query: 361 AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSEVVKIVAPLPPILRTHGESEDAGN 419
           AEDGGY+RP KPVPGDCGYYLHAHQL+L+HPKT+EVVKIVAPLPPILRT  E+ED  N
Sbjct: 362 AEDGGYERPMKPVPGDCGYYLHAHQLILYHPKTNEVVKIVAPLPPILRTRSEAEDFHN 369

BLAST of Carg02396 vs. ExPASy TrEMBL
Match: A0A1S3CPE8 (RNA pseudouridine synthase 5 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503310 PE=4 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 4.1e-171
Identity = 314/426 (73.71%), Postives = 334/426 (78.40%), Query Frame = 0

Query: 1   MLGRSFQVIGIPWPDLNDGLFYNDVVKPSDSGMSLIEFYSRKYKSSAPLEGLLLIINETY 60
           +LGRSFQVIGIPWPDLNDGLFYNDVVKP DSG SLI FYS KYKSSAPLEG L  I    
Sbjct: 2   LLGRSFQVIGIPWPDLNDGLFYNDVVKPPDSGKSLIHFYSCKYKSSAPLEGWLQRI---- 61

Query: 61  FTSITLQLVAANSERTDNDRWSGYHRSRNNSQVLHPGSFTPDLMVCSKVCITALGVFIYY 120
                                        N Q+   G    D                  
Sbjct: 62  ----------------------------KNEQITIDGEVITDPET--------------- 121

Query: 121 KICRIGSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL 180
            I RIGSE+VYRRLPWKEPDTP+ LE+LYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL
Sbjct: 122 -ILRIGSELVYRRLPWKEPDTPFSLEILYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQL 181

Query: 181 QWWSSKQSSSVSEELHPVPVHRLGRGTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNN 240
           QWWSS QS S+SEELHPVPVHRLGRGTSGILLCAKT+LARTQF+AYFADGTS V   RN+
Sbjct: 182 QWWSSDQSFSISEELHPVPVHRLGRGTSGILLCAKTKLARTQFAAYFADGTSSV--IRNS 241

Query: 241 TMEEGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVN 300
           T EEGTKRKISKIYRALV GIIHDDEVS+DQPIGRM+YPGVAQGLYVAT SGKPA SKVN
Sbjct: 242 TSEEGTKRKISKIYRALVTGIIHDDEVSVDQPIGRMQYPGVAQGLYVATPSGKPACSKVN 301

Query: 301 VLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLMGDPLYVAGGQPNCLHSELVDQSF 360
           VLERDEQG+HTLVQVEIQSGRPHQIRIHLSSIGHPL+GDPLYVAGGQPNCLHSE+V+QSF
Sbjct: 302 VLERDEQGEHTLVQVEIQSGRPHQIRIHLSSIGHPLLGDPLYVAGGQPNCLHSEVVNQSF 361

Query: 361 AEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPKTSE--------VVKIVAPLPPILRTHGE 419
           AEDGGY+RP KPVPGDCGYYLHAHQL+L+HPKT+E        VVKIVAPLPPILRT  E
Sbjct: 362 AEDGGYERPMKPVPGDCGYYLHAHQLILYHPKTNEKGFLVLLKVVKIVAPLPPILRTRSE 377

BLAST of Carg02396 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 443.7 bits (1140), Expect = 1.7e-124
Identity = 239/413 (57.87%), Postives = 283/413 (68.52%), Query Frame = 0

Query: 7   QVIGIPWPDLNDGLFYNDVVKPSDSGMSLI-EFYSRKYKSSAPLEGLLLIINETYFTSIT 66
           Q IG+PWP+LNDGL Y D +  S+S ++ + EFY  KYKSSAPL G +  I         
Sbjct: 6   QRIGLPWPELNDGLAYKDAISSSESELTTVSEFYFTKYKSSAPLLGWIQRI--------- 65

Query: 67  LQLVAANSERTDNDRWSGYHRSRNNSQVLHPGSFTPDLMVCSKVCITALGVFIYYKICRI 126
                                   N Q+   G    D                   + R 
Sbjct: 66  -----------------------QNGQIQIDGEVVKDPNT----------------LLRS 125

Query: 127 GSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQLQWWSS 186
           GS++VY RLPWKEPDTPY LEVLYEDD++IALNKPSGLQVLPGG+FQQRTVLTQLQW   
Sbjct: 126 GSKLVYSRLPWKEPDTPYSLEVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFG 185

Query: 187 KQSSSVS--EELHPVPVHRLGRGTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNNTME 246
           K  S +   E  HPVPVHRLGRGTSGILLCAKT+LA+T+ +AYFA+GTS VG + N   E
Sbjct: 186 KNDSYIGSRESPHPVPVHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVG-SGNLDQE 245

Query: 247 EGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVNVLE 306
            GT RK+SKIYRAL  GI+ +DEV I QPIG +RYPGVAQGLYVA+  GKPA+SKV VLE
Sbjct: 246 CGTGRKLSKIYRALADGIVEEDEVVIKQPIGVVRYPGVAQGLYVASPEGKPAFSKVFVLE 305

Query: 307 RDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLMGDPLYVAGGQPNCLHSELVDQ--SFA 366
           RD +   +LV+VEIQSGRPHQIRIHL+ +GHPL+GDPLYVAGGQP C   +LVD   +FA
Sbjct: 306 RDREKNCSLVKVEIQSGRPHQIRIHLAYMGHPLVGDPLYVAGGQPKCFDPDLVDDAAAFA 365

Query: 367 EDGGYQRPTKPVPGDCGYYLHAHQLVLHH-PKTSEVVKIVAPLPPILRTHGES 414
           EDGGY+RP + VPGDCGY+LHAHQ+ L +   T +VVKIVAPLPPIL+T  E+
Sbjct: 366 EDGGYRRPNQAVPGDCGYHLHAHQVELPNLLNTHKVVKIVAPLPPILQTRCET 369

BLAST of Carg02396 vs. TAIR 10
Match: AT3G52260.3 (Pseudouridine synthase family protein )

HSP 1 Score: 442.2 bits (1136), Expect = 4.8e-124
Identity = 242/430 (56.28%), Postives = 293/430 (68.14%), Query Frame = 0

Query: 7   QVIGIPWPDLNDGLFYNDVVKPSDSGMSLI-EFYSRKYKSSAPLEGLLLIINETYFTSIT 66
           Q IG+PWP+LNDGL Y D +  S+S ++ + EFY  KYKSSAPL G LL      ++S T
Sbjct: 6   QRIGLPWPELNDGLAYKDAISSSESELTTVSEFYFTKYKSSAPLLGFLLFF--FLYSSST 65

Query: 67  LQLVAANSERTDNDRWSGYHRSRNNSQVLHPGSFTPDLMVCSKVCITALGVFIYYKICRI 126
           +     +          G++ S+ +  +   G    D                   + R 
Sbjct: 66  IISFLLSILFNHLSFLGGFNESKTDRFIQIDGEVVKDPNT----------------LLRS 125

Query: 127 GSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQLQWWSS 186
           GS++VY RLPWKEPDTPY LEVLYEDD++IALNKPSGLQVLPGG+FQQRTVLTQLQW   
Sbjct: 126 GSKLVYSRLPWKEPDTPYSLEVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFG 185

Query: 187 KQSSSVS--EELHPVPVHRLGRGTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNNTME 246
           K  S +   E  HPVPVHRLGRGTSGILLCAKT+LA+T+ +AYFA+GTS VG + N   E
Sbjct: 186 KNDSYIGSRESPHPVPVHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVG-SGNLDQE 245

Query: 247 EGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVNVLE 306
            GT RK+SKIYRAL  GI+ +DEV I QPIG +RYPGVAQGLYVA+  GKPA+SKV VLE
Sbjct: 246 CGTGRKLSKIYRALADGIVEEDEVVIKQPIGVVRYPGVAQGLYVASPEGKPAFSKVFVLE 305

Query: 307 RDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLM-----------------GDPLYVAGG 366
           RD +   +LV+VEIQSGRPHQIRIHL+ +GHPL+                 GDPLYVAGG
Sbjct: 306 RDREKNCSLVKVEIQSGRPHQIRIHLAYMGHPLVEMTMFTGSECLRADQSAGDPLYVAGG 365

Query: 367 QPNCLHSELVDQ--SFAEDGGYQRPTKPVPGDCGYYLHAHQLVLHH-PKTSEVVKIVAPL 414
           QP C   +LVD   +FAEDGGY+RP + VPGDCGY+LHAHQ+ L +   T +VVKIVAPL
Sbjct: 366 QPKCFDPDLVDDAAAFAEDGGYRRPNQAVPGDCGYHLHAHQVELPNLLNTHKVVKIVAPL 416

BLAST of Carg02396 vs. TAIR 10
Match: AT3G52260.1 (Pseudouridine synthase family protein )

HSP 1 Score: 340.5 bits (872), Expect = 2.0e-93
Identity = 187/333 (56.16%), Postives = 220/333 (66.07%), Query Frame = 0

Query: 7   QVIGIPWPDLNDGLFYNDVVKPSDSGMSLI-EFYSRKYKSSAPLEGLLLIINETYFTSIT 66
           Q IG+PWP+LNDGL Y D +  S+S ++ + EFY  KYKSSAPL G +  I         
Sbjct: 6   QRIGLPWPELNDGLAYKDAISSSESELTTVSEFYFTKYKSSAPLLGWIQRI--------- 65

Query: 67  LQLVAANSERTDNDRWSGYHRSRNNSQVLHPGSFTPDLMVCSKVCITALGVFIYYKICRI 126
                                   N Q+   G    D                   + R 
Sbjct: 66  -----------------------QNGQIQIDGEVVKDPNT----------------LLRS 125

Query: 127 GSEVVYRRLPWKEPDTPYLLEVLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQLQWWSS 186
           GS++VY RLPWKEPDTPY LEVLYEDD++IALNKPSGLQVLPGG+FQQRTVLTQLQW   
Sbjct: 126 GSKLVYSRLPWKEPDTPYSLEVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFG 185

Query: 187 KQSSSVS--EELHPVPVHRLGRGTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNNTME 246
           K  S +   E  HPVPVHRLGRGTSGILLCAKT+LA+T+ +AYFA+GTS VG + N   E
Sbjct: 186 KNDSYIGSRESPHPVPVHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVG-SGNLDQE 245

Query: 247 EGTKRKISKIYRALVAGIIHDDEVSIDQPIGRMRYPGVAQGLYVATSSGKPAYSKVNVLE 306
            GT RK+SKIYRAL  GI+ +DEV I QPIG +RYPGVAQGLYVA+  GKPA+SKV VLE
Sbjct: 246 CGTGRKLSKIYRALADGIVEEDEVVIKQPIGVVRYPGVAQGLYVASPEGKPAFSKVFVLE 289

Query: 307 RDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPL 337
           RD +   +LV+VEIQSGRPHQIRIHL+ +GHPL
Sbjct: 306 RDREKNCSLVKVEIQSGRPHQIRIHLAYMGHPL 289

BLAST of Carg02396 vs. TAIR 10
Match: AT5G51140.1 (Pseudouridine synthase family protein )

HSP 1 Score: 83.2 bits (204), Expect = 5.7e-16
Identity = 58/204 (28.43%), Postives = 93/204 (45.59%), Query Frame = 0

Query: 147 VLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQLQWWSSKQSSSVSEELHPV-PVHRLGR 206
           +L+++ +++ + KP+ + V P G +++ T++  L             +L  + P+HRL R
Sbjct: 138 ILHQEPDVVTVCKPASVPVHPCGQYRKNTIVGIL---------DAEHDLGTLFPIHRLDR 197

Query: 207 GTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNNTMEEGTKRKISKIYRALVAGIIHDD 266
             SG+L+ A+T      F      G                   + K Y A V G+  +D
Sbjct: 198 LVSGLLIIARTAAKADFFRQQIEGG------------------MVKKRYIAKVIGVFPED 257

Query: 267 EVSIDQPIGRMRYPGVAQGLYVATS------SGKPAYSKVNVLERDEQGKHTLVQVEIQS 326
           E+ +D  I      G +      +S       GKPA +K   +  D  G H+LV  E  +
Sbjct: 258 EMIVDANINYNGSEGRSTAEDANSSGDDKKVKGKPACTKFTRI--DTNGTHSLVSCEPVT 312

Query: 327 GRPHQIRIHLSSIGHPLMGDPLYV 344
           GR HQIR+HL   GHP+  DPLY+
Sbjct: 318 GRTHQIRVHLQYTGHPIANDPLYL 312

BLAST of Carg02396 vs. TAIR 10
Match: AT5G51140.2 (Pseudouridine synthase family protein )

HSP 1 Score: 83.2 bits (204), Expect = 5.7e-16
Identity = 58/204 (28.43%), Postives = 93/204 (45.59%), Query Frame = 0

Query: 147 VLYEDDEMIALNKPSGLQVLPGGVFQQRTVLTQLQWWSSKQSSSVSEELHPV-PVHRLGR 206
           +L+++ +++ + KP+ + V P G +++ T++  L             +L  + P+HRL R
Sbjct: 138 ILHQEPDVVTVCKPASVPVHPCGQYRKNTIVGIL---------DAEHDLGTLFPIHRLDR 197

Query: 207 GTSGILLCAKTRLARTQFSAYFADGTSCVGRNRNNTMEEGTKRKISKIYRALVAGIIHDD 266
             SG+L+ A+T      F      G                   + K Y A V G+  +D
Sbjct: 198 LVSGLLIIARTAAKADFFRQQIEGG------------------MVKKRYIAKVIGVFPED 257

Query: 267 EVSIDQPIGRMRYPGVAQGLYVATS------SGKPAYSKVNVLERDEQGKHTLVQVEIQS 326
           E+ +D  I      G +      +S       GKPA +K   +  D  G H+LV  E  +
Sbjct: 258 EMIVDANINYNGSEGRSTAEDANSSGDDKKVKGKPACTKFTRI--DTNGTHSLVSCEPVT 312

Query: 327 GRPHQIRIHLSSIGHPLMGDPLYV 344
           GR HQIR+HL   GHP+  DPLY+
Sbjct: 318 GRTHQIRVHLQYTGHPIANDPLYL 312