Carg02352 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg02352
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	CASP-like protein
Location	Carg_Chr15: 1941600 .. 1944696 (-)
RNA-Seq Expression	Carg02352
Synteny	Carg02352

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAGTTCAAGTGGTGCAAGGGTGACTTGTCTTATATTGAGAATCCTAACCTTCGTGTTTCTATTCATCTCCTTCTTCGTTCTCGTCACCACGTCTCAAACCGTTGAATCGGTAAAGTTTCATTTCAACAACTTCCATACATATAGGTACGTGCTTGCTACAATCATCATCGGGATCGTATTCAACCTCTTGCAAATCGCATTCTCCCTCTTTAATATCGTTAAAGGTGCAACCGGTACCATACTATTTGATTTCTTTGGCGACAAGGTACGTTATACACACACATCTAGTGTCATGTTTGATTCTATTCGAATATGTGGCCCTAAACGCGTCGGTTTTGGTGCAGTTTTTGTCATACCTTCTAGCCACAGGCACAGCAGCTGGGTTTGGACTATCAGTGGATTTGCAAACATTAGACCCAGATGACTACTTTGGAACGTTCTTCGACAAGGCATATGCGGCCTCGGCTCTTCTTTTGTTCGCGTTCTTTTGTTCGGCGGCGGTCTCTATTATCTCTTCCTTTGCACTCTCGAAGCGACCTTAATTAATTAATCTTTGGTTCTTTAATTGTTTCCATGTTCTTTTTTTCTTTCGTCTTAAGATCATGTCGTTCTTATTGGGTTTTGTTTGATGATTTGTTACAATTCTTTTTCTCCGAAATTTTATTGGATTGTTTAATATAAAAGTTTATAAATATTTTTCGAGTCAAACTGTTCGAGCAATTCAACACGGACTTATGATGAATACAATGAAACCGAGTGATCGAGTGTTCCGTACAAGTATTAAAATTTGATATCAATTAAAAAATGGTTCAAAATAAGTGTTTGATAAAATAGATTTATAAATTATTTATTATTGAATATAAAATAATTAATTTGTGAACATTTTTGTTTATAAAAATATTATTTTACAATATTCATTTAGTTTATAATACAAAATGAGTTTACAACATAAAACATTGTATTTATTAATTTATTATTTTTAAATATAAATCTTCAATTTAAAATTTTAATAAAAGTTATCCGTATTATTTAGATTACAAAATTTAAAAACAGTTTTAAAAAACACATTCTACATTAAAAAAAAAACATGACGATTAACAAATTTCGTACCAACCAACCTCGAATTTTCTTTGTTACATTTATCCGTTAATAATCAGTTATATCACGAAAAATAATTCATTTAAAAAAAAATAAAGAAAGCACATTTCATTTATTTATTATCCTTTTAATTTTATCTATAATAAGTTCTTCCATTTCAGCAAACATGTCATATGATCGAGATTGATTAAACGTGTTAGGAAACATTTGAAATTTCATCAACTTGTCATATAAATATAAAATAAAAAAAAAACTTTAATTATTTATTAAACATTTTAAAATTAAACATGGTATTATTAATTAAGAAGGCTAGAGATTTTTAATCTCCATCTAACAACGTTATGAATCATTAATGAGGGAGTAGGTCAAACTATAGAAAAAGTCAAAGGAGAATAAAATATATTTTAAAGACAACGACTTGAAACATGTCTGGTTATTTGACAAAAGTGTTGGGAGAGACGACTTGGCCGCCATTTCCGGCCGGTGAACCTTCCCACCACGTTCACTTGGGAGTTCATCAAAAGTAAACCATCGACATGGACAATCTAGGATTTCTTTATTATTATATTCATAAGTTTTTTTTTTAATTATGTATTTAATAAAATTTTGATAACTTAACAAGTTTTTTTTAAAAATTAATAACCCTATTAAATATATATTTAAAATTCAATAAATATAGTATGTGCATGTCCTAAATTCAATTAATTTTTATTAAATTAAAATATAATATAATAGATTTATAAATTAATAATTAAAAGACTTTGCCCATTATAATTTATTCCAAGTCAAAGCAATAACGAGGAAGTAGCCATTATTACCTCATCCACTTGCTTCAATACTCAAAAGTCAACTTGTACAATTGCTTCTAGTTTTTCTTGCATTTACACAAAATCGATGTAAACCCTTTTCTAAAAATCATATTGTTCTAATTTTTTATATTTGCCTGACCGAAACTAATACAAGCAAATCGGAGGACGAACGTCCCATTCTTTTCCTTCTTTATGTTGAGGATTATTTGGAGATTTAGTGGAAGATCGTGAGTTTATAAGTGAGAAATAATATCAATATGAGACCTTCTGAGAAAGCTCATAATAAGGTGATGAGAGCTTATGCTCAAAGTGGACGATTCTAACACTTTGTTTGGGTTTTTTCGGAGAGAAAAACAAAGACGTAGACGTGGGTAAAGGTTAACAACGCGGAGAAAATGGAATCAAAGGAGAAAAATGAGGAAGTAGTAGGCAAGTTGGTGTTATTTTTTTGGTCTAACTTATGATGTGAACAAAGACCTGTTTGTTTGAATATTCAAAGATGTTTTTTACACGTATTGAAGTTCCATGTGGAAACAAAAAGCAATGGGCATTTTTTGGGGTATAAAAGGTGAACCAGTTAGAGATATTAAATTCATTACCAAGATCAGCAAAAATGGAAGCTAAAATGGGTACCAAAATTGCTTCTTTTGTGCTCAGAGTTCTCACCTTCGTCTTCCTCTTAATTTCCATCATAGTTCTTGGAACCACTTCCAAGACCGTAGGAGATGACAAGGTTCAATTCCATAATGTCAATTCCTATAGGTATTTATTTATTTATTTCCATTTATTTTCATTTATTTCTATTGGGTTTCATGATTTATTTATTATTATTATTAAATTTGAAAAAAAAAAAAAATCAAAATTTGAAGGTTTGCAATGGCGACGATCATCATTGGAGGAGCATTCAACCTCTTGCAAATAGCCTTAGCCCTTTATCGTCTCATAACCAAAACCGACGGCGGTGTTTTATTCGACTTCTTCGGTGATAAGGTTTTGTCGTACTTTCTGCTGGCGGGAGCGGCGGCGGCGCTGGGTTCTACCATAGACTTGAAGGCCAATATTGACTGGTTGAACTCGTTTTTCGACCAAGGCAACGCCGCTGCGGCTCTTCTTCTTCTTGCTTTCCTATGCAGTGCTATAGTTTCTGTTCTTTCCTCTTTTGCCCTTTCTCACAAACCTACTTAG

mRNA sequence

ATGGAAAGTTCAAGTGGTGCAAGGGTGACTTGTCTTATATTGAGAATCCTAACCTTCGTGTTTCTATTCATCTCCTTCTTCGTTCTCGTCACCACGTCTCAAACCGTTGAATCGGTAAAGTTTCATTTCAACAACTTCCATACATATAGGTACGTGCTTGCTACAATCATCATCGGGATCGTATTCAACCTCTTGCAAATCGCATTCTCCCTCTTTAATATCGTTAAAGGTGCAACCGGTACCATACTATTTGATTTCTTTGGCGACAAGTTTTTGTCATACCTTCTAGCCACAGGCACAGCAGCTGGGTTTGGACTATCAGTGGATTTGCAAACATTAGACCCAGATGACTACTTTGGAACGTTCTTCGACAAGGCATATGCGGCCTCGGCTCTTCTTTTGTTCGCAGTTCTCACCTTCGTCTTCCTCTTAATTTCCATCATAGTTCTTGGAACCACTTCCAAGACCGTAGGAGATGACAAGGTTCAATTCCATAATGTCAATTCCTATAGGTTTGCAATGGCGACGATCATCATTGGAGGAGCATTCAACCTCTTGCAAATAGCCTTAGCCCTTTATCGTCTCATAACCAAAACCGACGGCGGTGTTTTATTCGACTTCTTCGGTGATAAGGTTTTGTCGTACTTTCTGCTGGCGGGAGCGGCGGCGGCGCTGGGTTCTACCATAGACTTGAAGGCCAATATTGACTGGTTGAACTCGTTTTTCGACCAAGGCAACGCCGCTGCGGCTCTTCTTCTTCTTGCTTTCCTATGCAGTGCTATAGTTTCTGTTCTTTCCTCTTTTGCCCTTTCTCACAAACCTACTTAG

Coding sequence (CDS)

ATGGAAAGTTCAAGTGGTGCAAGGGTGACTTGTCTTATATTGAGAATCCTAACCTTCGTGTTTCTATTCATCTCCTTCTTCGTTCTCGTCACCACGTCTCAAACCGTTGAATCGGTAAAGTTTCATTTCAACAACTTCCATACATATAGGTACGTGCTTGCTACAATCATCATCGGGATCGTATTCAACCTCTTGCAAATCGCATTCTCCCTCTTTAATATCGTTAAAGGTGCAACCGGTACCATACTATTTGATTTCTTTGGCGACAAGTTTTTGTCATACCTTCTAGCCACAGGCACAGCAGCTGGGTTTGGACTATCAGTGGATTTGCAAACATTAGACCCAGATGACTACTTTGGAACGTTCTTCGACAAGGCATATGCGGCCTCGGCTCTTCTTTTGTTCGCAGTTCTCACCTTCGTCTTCCTCTTAATTTCCATCATAGTTCTTGGAACCACTTCCAAGACCGTAGGAGATGACAAGGTTCAATTCCATAATGTCAATTCCTATAGGTTTGCAATGGCGACGATCATCATTGGAGGAGCATTCAACCTCTTGCAAATAGCCTTAGCCCTTTATCGTCTCATAACCAAAACCGACGGCGGTGTTTTATTCGACTTCTTCGGTGATAAGGTTTTGTCGTACTTTCTGCTGGCGGGAGCGGCGGCGGCGCTGGGTTCTACCATAGACTTGAAGGCCAATATTGACTGGTTGAACTCGTTTTTCGACCAAGGCAACGCCGCTGCGGCTCTTCTTCTTCTTGCTTTCCTATGCAGTGCTATAGTTTCTGTTCTTTCCTCTTTTGCCCTTTCTCACAAACCTACTTAG

Protein sequence

MESSSGARVTCLILRILTFVFLFISFFVLVTTSQTVESVKFHFNNFHTYRYVLATIIIGIVFNLLQIAFSLFNIVKGATGTILFDFFGDKFLSYLLATGTAAGFGLSVDLQTLDPDDYFGTFFDKAYAASALLLFAVLTFVFLLISIIVLGTTSKTVGDDKVQFHNVNSYRFAMATIIIGGAFNLLQIALALYRLITKTDGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLKANIDWLNSFFDQGNAAAALLLLAFLCSAIVSVLSSFALSHKPT

Homology

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BLAST of Carg02352 vs. NCBI nr
Match: KAG7016238.1 (CASP-like protein 4D1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 512.3 bits (1318), Expect = 2.6e-141
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0


Query: 1   MESSSGARVTCLILRILTFVFLFISFFVLVTTSQTVESVKFHFNNFHTYRYVLATIIIGI 60
           MESSSGARVTCLILRILTFVFLFISFFVLVTTSQTVESVKFHFNNFHTYRYVLATIIIGI
Sbjct: 1   MESSSGARVTCLILRILTFVFLFISFFVLVTTSQTVESVKFHFNNFHTYRYVLATIIIGI 60

Query: 61  VFNLLQIAFSLFNIVKGATGTILFDFFGDKFLSYLLATGTAAGFGLSVDLQTLDPDDYFG 120
           VFNLLQIAFSLFNIVKGATGTILFDFFGDKFLSYLLATGTAAGFGLSVDLQTLDPDDYFG
Sbjct: 61  VFNLLQIAFSLFNIVKGATGTILFDFFGDKFLSYLLATGTAAGFGLSVDLQTLDPDDYFG 120

Query: 121 TFFDKAYAASALLLFAVLTFVFLLISIIVLGTTSKTVGDDKVQFHNVNSYRFAMATIIIG 180
           TFFDKAYAASALLLFAVLTFVFLLISIIVLGTTSKTVGDDKVQFHNVNSYRFAMATIIIG
Sbjct: 121 TFFDKAYAASALLLFAVLTFVFLLISIIVLGTTSKTVGDDKVQFHNVNSYRFAMATIIIG 180

Query: 181 GAFNLLQIALALYRLITKTDGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLKANIDWLNS 240
           GAFNLLQIALALYRLITKTDGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLKANIDWLNS
Sbjct: 181 GAFNLLQIALALYRLITKTDGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLKANIDWLNS 240

Query: 241 FFDQGNAAAALLLLAFLCSAIVSVLSSFALSHKPT 276
           FFDQGNAAAALLLLAFLCSAIVSVLSSFALSHKPT
Sbjct: 241 FFDQGNAAAALLLLAFLCSAIVSVLSSFALSHKPT 275

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BLAST of Carg02352 vs. NCBI nr
Match: XP_023551429.1 (CASP-like protein 4D1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 260.0 bits (663), Expect = 2.3e-65
Identity = 139/145 (95.86%), Postives = 141/145 (97.24%), Query Frame = 0


Query: 131 ALLLFAVLTFVFLLISIIVLGTTSKTVGDDKVQFHNVNSYRFAMATIIIGGAFNLLQIAL 190
           A  +  VLTFVFLLISIIVLGTTSKTVGDDKVQFHNVNSYRFAMATIIIGGAFNLLQIAL
Sbjct: 10  ASFVLRVLTFVFLLISIIVLGTTSKTVGDDKVQFHNVNSYRFAMATIIIGGAFNLLQIAL 69

Query: 191 ALYRLITKTDGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLKANIDWLNSFFDQGNAAAA 250
           ALYRLITKTDGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLKANIDWLNSFFDQGNAAAA
Sbjct: 70  ALYRLITKTDGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLKANIDWLNSFFDQGNAAAA 129

Query: 251 LLLLAFLCSAIVSVLSSFALSHKPT 276
           LLLLAFLCSA+VSVLSSFALSHKPT
Sbjct: 130 LLLLAFLCSAVVSVLSSFALSHKPT 154

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BLAST of Carg02352 vs. NCBI nr
Match: XP_022992769.1 (CASP-like protein 4D1 [Cucurbita maxima])

HSP 1 Score: 257.3 bits (656), Expect = 1.5e-64
Identity = 137/145 (94.48%), Postives = 141/145 (97.24%), Query Frame = 0


Query: 131 ALLLFAVLTFVFLLISIIVLGTTSKTVGDDKVQFHNVNSYRFAMATIIIGGAFNLLQIAL 190
           A  +  VLTFVFLLISIIVLGTTSKTVGD+K+QFHNVNSYRFAMATIIIGGAFNLLQIAL
Sbjct: 10  ASFVLRVLTFVFLLISIIVLGTTSKTVGDEKLQFHNVNSYRFAMATIIIGGAFNLLQIAL 69

Query: 191 ALYRLITKTDGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLKANIDWLNSFFDQGNAAAA 250
           ALYRLITKTDGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLKANIDWLNSFFDQGNAAAA
Sbjct: 70  ALYRLITKTDGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLKANIDWLNSFFDQGNAAAA 129

Query: 251 LLLLAFLCSAIVSVLSSFALSHKPT 276
           LLLLAFLCSA+VSVLSSFALSHKPT
Sbjct: 130 LLLLAFLCSAVVSVLSSFALSHKPT 154

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BLAST of Carg02352 vs. NCBI nr
Match: XP_022939465.1 (CASP-like protein 4D1 [Cucurbita moschata])

HSP 1 Score: 256.1 bits (653), Expect = 3.3e-64
Identity = 137/145 (94.48%), Postives = 141/145 (97.24%), Query Frame = 0


Query: 131 ALLLFAVLTFVFLLISIIVLGTTSKTVGDDKVQFHNVNSYRFAMATIIIGGAFNLLQIAL 190
           A  +  VLTFVFLLISIIVLGTTSKTVGD+K+QFHNVNSYRFAMATIIIGGAFNLLQIAL
Sbjct: 10  ASFVLRVLTFVFLLISIIVLGTTSKTVGDEKLQFHNVNSYRFAMATIIIGGAFNLLQIAL 69

Query: 191 ALYRLITKTDGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLKANIDWLNSFFDQGNAAAA 250
           ALYRLITK+DGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLKANIDWLNSFFDQGNAAAA
Sbjct: 70  ALYRLITKSDGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLKANIDWLNSFFDQGNAAAA 129

Query: 251 LLLLAFLCSAIVSVLSSFALSHKPT 276
           LLLLAFLCSAIVSVLSSFALSHKPT
Sbjct: 130 LLLLAFLCSAIVSVLSSFALSHKPT 154

[Close]

BLAST of Carg02352 vs. NCBI nr
Match: XP_023549516.1 (CASP-like protein 4D1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 253.1 bits (645), Expect = 2.8e-63
Identity = 141/155 (90.97%), Postives = 143/155 (92.26%), Query Frame = 0


Query: 1   MESSSGARVTCLILRILTFVFLFISFFVLVTTSQTVESVKFHFNNFHTYRYVLATIIIGI 60
           ME+SSGARVTCLILRILTFVFLFISFFVLVTTSQTVESVKFHFNNFHTYRYVLATIIIGI
Sbjct: 1   MENSSGARVTCLILRILTFVFLFISFFVLVTTSQTVESVKFHFNNFHTYRYVLATIIIGI 60

Query: 61  VFNLLQIAFSLFNIVKGATGTILFDFFGDKFLSYLLATGTAAGFGLSVDLQTLDPDDYFG 120
           VFNLLQIAFSLFNIVKGATGTILFDFFGDKFLSYLLATGTAAGFGLSVDLQTLDPDDYFG
Sbjct: 61  VFNLLQIAFSLFNIVKGATGTILFDFFGDKFLSYLLATGTAAGFGLSVDLQTLDPDDYFG 120

Query: 121 TFFDKAYAASALLLFAVLTFVFLLISIIVLGTTSK 156
           TFFDKAYAASALLLFA   F    +SII     SK
Sbjct: 121 TFFDKAYAASALLLFAF--FCSAAVSIISSFALSK 153

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BLAST of Carg02352 vs. ExPASy Swiss-Prot
Match: C6T1Z6 (CASP-like protein 4D1 OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 2.0e-27
Identity = 69/151 (45.70%), Postives = 109/151 (72.19%), Query Frame = 0


Query: 130 SALLLFAVLTFVFLLISIIVLGTTSKTVGDD--KVQFHNVNSYRFAMATIIIGGAFNLLQ 189
           + LLL  VLTFVFLLI++I++    +T  +   +++F+++ +YR+ ++TIIIG A+NLLQ
Sbjct: 19  TVLLLLRVLTFVFLLIALILIAIVKQTDDETGVEIKFNDIYAYRYMISTIIIGFAYNLLQ 78

Query: 190 IALALYRLIT-----KTDGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLKANIDWLNSFF 249
           +AL+++ +++       DGG LFDFFGDK++SY L++G+AA  G T++L   +   NSF 
Sbjct: 79  MALSIFTVVSGNRVLSGDGGYLFDFFGDKIISYLLISGSAAGFGVTVELGRGVP-SNSFM 138

Query: 250 DQGNAAAALLLLAFLCSAIVSVLSSFALSHK 274
           D+ NA+A+LLL+AFL +A+ S  +SFAL  K
Sbjct: 139 DKANASASLLLIAFLFTAVASTFTSFALPKK 168

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BLAST of Carg02352 vs. ExPASy Swiss-Prot
Match: B9SXY8 (CASP-like protein 4D1 OS=Ricinus communis OX=3988 GN=RCOM_1206790 PE=3 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.6e-24
Identity = 68/152 (44.74%), Postives = 102/152 (67.11%), Query Frame = 0


Query: 131 ALLLFAVLTFVFLLISIIVLGTTSKTVGDD----KVQFHNVNSYRFAMATIIIGGAFNLL 190
           A L+  +LTF+FL+ S+++L T + T+  D    KV F +V +YR+ +ATI+IG A+ +L
Sbjct: 13  AALILRILTFIFLIASLVILTTNTATLELDLVEVKVHFKDVYAYRYMLATIVIGLAYTVL 72

Query: 191 QIALALYRLIT-----KTDGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLK---ANIDWL 250
           QIA  LY + T       DG + FDFFGDKV+SY L+ GAAA   ST D+K   +     
Sbjct: 73  QIAFTLYYVATGNRMMSGDGNLAFDFFGDKVISYILVTGAAAGFASTKDIKPVFSGSGDF 132

Query: 251 NSFFDQGNAAAALLLLAFLCSAIVSVLSSFAL 271
           ++F ++G A+A+LLL+ F+C+A++SV SS+AL
Sbjct: 133 DAFINKGYASASLLLIGFVCTAVLSVFSSYAL 164

[Close]

BLAST of Carg02352 vs. ExPASy Swiss-Prot
Match: B9NBE5 (CASP-like protein 4D1 OS=Populus trichocarpa OX=3694 GN=POPTR_0010s21230g PE=2 SV=2)

HSP 1 Score: 110.2 bits (274), Expect = 3.9e-23
Identity = 67/153 (43.79%), Postives = 99/153 (64.71%), Query Frame = 0


Query: 133 LLFAVLTFVFLLISIIVLGTTSKT----VGDDKVQFHNVNSYRFAMATIIIGGAFNLLQI 192
           L   VLTF FL++S++++ T + T    + + KV+  +  SYR+ +A I  G  + +LQI
Sbjct: 24  LFLRVLTFAFLMVSLVIMTTNTGTIEIGIDEFKVRSKDFYSYRYMLAAIAFGLTYTILQI 83

Query: 193 ALALYRLI------TKTDGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLKANIDWL--NS 252
           AL L  +       T  DG ++FDF+GDKV+SY L  GAAAA G+T +LK  +  L  + 
Sbjct: 84  ALTLNHISKRNGAQTSGDGNLVFDFYGDKVVSYILATGAAAAFGATKELKTQLAGLGGDK 143

Query: 253 FFDQGNAAAALLLLAFLCSAIVSVLSSFALSHK 274
           FF++G A+A+LLLL F+C+AI+SV SS+AL  K
Sbjct: 144 FFNKGYASASLLLLGFVCTAILSVFSSYALPKK 176

[Close]

BLAST of Carg02352 vs. ExPASy Swiss-Prot
Match: A1XGB4 (CASP-like protein PIMP1 OS=Capsicum annuum OX=4072 GN=PIMP1 PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.9e-22
Identity = 66/143 (46.15%), Postives = 94/143 (65.73%), Query Frame = 0


Query: 12  LILRILTFVFLFISFFVLVTTSQTVES----VKFHFNNFHTYRYVLATIIIGIVFNLLQI 71
           LI+RILT + L ISF V+ T +QTV +    VK  F +F+ YRY++AT+IIG+ + LLQI
Sbjct: 15  LIVRILTLICLLISFIVIATNNQTVSTVAGDVKIKFKDFYAYRYLIATVIIGMAYTLLQI 74

Query: 72  AFSLFNIVK----GATGTILFDFFGDKFLSYLLATGTAAGFGLSVDLQTLDPDDYFGTFF 131
           AFS+  +      G  G +LFDF+GDKF+SY L TG AA FG++ DL+ L+  D +  F 
Sbjct: 75  AFSISLLTTGNRIGGEGFLLFDFYGDKFISYFLVTGAAASFGMTQDLKQLEGSDNYSKFL 134

Query: 132 DKAYAASALLL----FAVLTFVF 143
           + + AA++L L    FAV + +F
Sbjct: 135 NTSNAAASLCLIGFFFAVASSIF 157

[Close]

BLAST of Carg02352 vs. ExPASy Swiss-Prot
Match: Q56X75 (CASP-like protein 4D2 OS=Arabidopsis thaliana OX=3702 GN=At2g39518 PE=2 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.0e-19
Identity = 61/151 (40.40%), Postives = 93/151 (61.59%), Query Frame = 0


Query: 8   RVTCLILRILTFVFLFISFFVLVTTSQTVES----VKFHFNNFHTYRYVLATIIIGIVFN 67
           +V  L+LR+LT VFL I+  +L T S T+ S    +KFHF + + YRY+L+  +IG+V+ 
Sbjct: 14  KVLLLLLRVLTGVFLVIALIILSTNSVTIVSQGSALKFHFKDVYAYRYMLSAAVIGLVYA 73

Query: 68  LLQIAFSLFNIVKGATGTILF--DFFGDKFLSYLLATGTAAGFGLSVDLQ-------TLD 127
           ++Q+ F++     G      +  DF+GDK +SYL+ATG+AAGFG++ DL+        LD
Sbjct: 74  VIQLFFTISEFATGVKNPFNYQLDFYGDKLISYLVATGSAAGFGVTKDLKDTFLALVALD 133

Query: 128 PDDYFGTFFDKAYAASALLLFAVLTFVFLLI 146
             D    FF K YA+++LLLFA +    L +
Sbjct: 134 STDPVDKFFSKGYASASLLLFAFICLAVLSV 164

[Close]

BLAST of Carg02352 vs. ExPASy TrEMBL
Match: A0A6J1JYE7 (CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111489007 PE=3 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 7.3e-65
Identity = 137/145 (94.48%), Postives = 141/145 (97.24%), Query Frame = 0


Query: 131 ALLLFAVLTFVFLLISIIVLGTTSKTVGDDKVQFHNVNSYRFAMATIIIGGAFNLLQIAL 190
           A  +  VLTFVFLLISIIVLGTTSKTVGD+K+QFHNVNSYRFAMATIIIGGAFNLLQIAL
Sbjct: 10  ASFVLRVLTFVFLLISIIVLGTTSKTVGDEKLQFHNVNSYRFAMATIIIGGAFNLLQIAL 69

Query: 191 ALYRLITKTDGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLKANIDWLNSFFDQGNAAAA 250
           ALYRLITKTDGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLKANIDWLNSFFDQGNAAAA
Sbjct: 70  ALYRLITKTDGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLKANIDWLNSFFDQGNAAAA 129

Query: 251 LLLLAFLCSAIVSVLSSFALSHKPT 276
           LLLLAFLCSA+VSVLSSFALSHKPT
Sbjct: 130 LLLLAFLCSAVVSVLSSFALSHKPT 154

[Close]

BLAST of Carg02352 vs. ExPASy TrEMBL
Match: A0A6J1FMS6 (CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111445361 PE=3 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 1.6e-64
Identity = 137/145 (94.48%), Postives = 141/145 (97.24%), Query Frame = 0


Query: 131 ALLLFAVLTFVFLLISIIVLGTTSKTVGDDKVQFHNVNSYRFAMATIIIGGAFNLLQIAL 190
           A  +  VLTFVFLLISIIVLGTTSKTVGD+K+QFHNVNSYRFAMATIIIGGAFNLLQIAL
Sbjct: 10  ASFVLRVLTFVFLLISIIVLGTTSKTVGDEKLQFHNVNSYRFAMATIIIGGAFNLLQIAL 69

Query: 191 ALYRLITKTDGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLKANIDWLNSFFDQGNAAAA 250
           ALYRLITK+DGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLKANIDWLNSFFDQGNAAAA
Sbjct: 70  ALYRLITKSDGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLKANIDWLNSFFDQGNAAAA 129

Query: 251 LLLLAFLCSAIVSVLSSFALSHKPT 276
           LLLLAFLCSAIVSVLSSFALSHKPT
Sbjct: 130 LLLLAFLCSAIVSVLSSFALSHKPT 154

[Close]

BLAST of Carg02352 vs. ExPASy TrEMBL
Match: A0A6J1JQU5 (CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111489006 PE=3 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 2.3e-63
Identity = 140/155 (90.32%), Postives = 143/155 (92.26%), Query Frame = 0


Query: 1   MESSSGARVTCLILRILTFVFLFISFFVLVTTSQTVESVKFHFNNFHTYRYVLATIIIGI 60
           ME+SSGARVTCLILRILTFVFLFISFFVLVTTSQTVESVKFHFNNFHTYRYVLATIIIGI
Sbjct: 1   MENSSGARVTCLILRILTFVFLFISFFVLVTTSQTVESVKFHFNNFHTYRYVLATIIIGI 60

Query: 61  VFNLLQIAFSLFNIVKGATGTILFDFFGDKFLSYLLATGTAAGFGLSVDLQTLDPDDYFG 120
           VFNLLQIAFSLFNIVKGATGTILFDFFGDKFLSYLLATGTAAGFGLSVDLQTLDPDDYFG
Sbjct: 61  VFNLLQIAFSLFNIVKGATGTILFDFFGDKFLSYLLATGTAAGFGLSVDLQTLDPDDYFG 120

Query: 121 TFFDKAYAASALLLFAVLTFVFLLISIIVLGTTSK 156
           TFFDKAYAASALLLFA   F    +SI+     SK
Sbjct: 121 TFFDKAYAASALLLFAF--FCSAAVSILSSFALSK 153

[Close]

BLAST of Carg02352 vs. ExPASy TrEMBL
Match: A0A6J1FGY1 (CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111445381 PE=3 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 4.0e-63
Identity = 140/155 (90.32%), Postives = 142/155 (91.61%), Query Frame = 0


Query: 1   MESSSGARVTCLILRILTFVFLFISFFVLVTTSQTVESVKFHFNNFHTYRYVLATIIIGI 60
           MESSSGARVTCLILRILTFVFLFISFFVLVTTSQTVESVKFHFNNFHTYRYVLATIIIG+
Sbjct: 1   MESSSGARVTCLILRILTFVFLFISFFVLVTTSQTVESVKFHFNNFHTYRYVLATIIIGV 60

Query: 61  VFNLLQIAFSLFNIVKGATGTILFDFFGDKFLSYLLATGTAAGFGLSVDLQTLDPDDYFG 120
           VFNLLQIAFSL NIVKGATGTILFDFFGDKFLSYLLATGTAAGFGLSVDLQTLDPDDYFG
Sbjct: 61  VFNLLQIAFSLLNIVKGATGTILFDFFGDKFLSYLLATGTAAGFGLSVDLQTLDPDDYFG 120

Query: 121 TFFDKAYAASALLLFAVLTFVFLLISIIVLGTTSK 156
           TFFDKAYAASALLLFA   F    +SII     SK
Sbjct: 121 TFFDKAYAASALLLFAF--FCSAAVSIISSFALSK 153

[Close]

BLAST of Carg02352 vs. ExPASy TrEMBL
Match: A0A1S3C911 (CASP-like protein OS=Cucumis melo OX=3656 GN=LOC103498157 PE=3 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 3.3e-57
Identity = 121/144 (84.03%), Postives = 135/144 (93.75%), Query Frame = 0


Query: 131 ALLLFAVLTFVFLLISIIVLGTTSKTVGDDKVQFHNVNSYRFAMATIIIGGAFNLLQIAL 190
           A  +  VLTFVFLL+SIIVLGT SKT+G+D+V FHNVNSYR+AMATIIIGGAFNLLQIAL
Sbjct: 10  ASFVLRVLTFVFLLVSIIVLGTNSKTIGNDEVHFHNVNSYRYAMATIIIGGAFNLLQIAL 69

Query: 191 ALYRLITKTDGGVLFDFFGDKVLSYFLLAGAAAALGSTIDLKANIDWLNSFFDQGNAAAA 250
           ALYRL+TKTDG +LFDF+GDKVLSYFLLAGAAAALGS++DLKAN+D LNSFFDQGNAAAA
Sbjct: 70  ALYRLVTKTDGSILFDFYGDKVLSYFLLAGAAAALGSSVDLKANMDMLNSFFDQGNAAAA 129

Query: 251 LLLLAFLCSAIVSVLSSFALSHKP 275
           LLLLAFLCSAI+SVLSS ALS+KP
Sbjct: 130 LLLLAFLCSAIISVLSSLALSNKP 153

[Close]

BLAST of Carg02352 vs. TAIR 10
Match: AT2G39518.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 97.8 bits (242), Expect = 1.4e-20
Identity = 61/151 (40.40%), Postives = 93/151 (61.59%), Query Frame = 0


Query: 8   RVTCLILRILTFVFLFISFFVLVTTSQTVES----VKFHFNNFHTYRYVLATIIIGIVFN 67
           +V  L+LR+LT VFL I+  +L T S T+ S    +KFHF + + YRY+L+  +IG+V+ 
Sbjct: 14  KVLLLLLRVLTGVFLVIALIILSTNSVTIVSQGSALKFHFKDVYAYRYMLSAAVIGLVYA 73

Query: 68  LLQIAFSLFNIVKGATGTILF--DFFGDKFLSYLLATGTAAGFGLSVDLQ-------TLD 127
           ++Q+ F++     G      +  DF+GDK +SYL+ATG+AAGFG++ DL+        LD
Sbjct: 74  VIQLFFTISEFATGVKNPFNYQLDFYGDKLISYLVATGSAAGFGVTKDLKDTFLALVALD 133

Query: 128 PDDYFGTFFDKAYAASALLLFAVLTFVFLLI 146
             D    FF K YA+++LLLFA +    L +
Sbjct: 134 STDPVDKFFSKGYASASLLLFAFICLAVLSV 164

[Close]

BLAST of Carg02352 vs. TAIR 10
Match: AT2G39530.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 94.7 bits (234), Expect = 1.2e-19
Identity = 61/151 (40.40%), Postives = 92/151 (60.93%), Query Frame = 0


Query: 8   RVTCLILRILTFVFLFISFFVLVTTSQTVE----SVKFHFNNFHTYRYVLATIIIGIVFN 67
           R   L+LR+LT  FL I+  ++ T + T+E    S+K  FN+ + YRY+L+  +IG+V+ 
Sbjct: 14  RTVLLLLRVLTAAFLLITVVLISTNTVTLEISSTSIKLPFNDVYAYRYMLSAAVIGLVYA 73

Query: 68  LLQIAFSLFNIVKGATG--TILFDFFGDKFLSYLLATGTAAGFGLSVDLQ-------TLD 127
           ++Q+  ++     G T   T  FDF+GDK +SYLLATG+AAGFG+S DL+         D
Sbjct: 74  VVQLFLTISQFATGKTHPLTYQFDFYGDKVISYLLATGSAAGFGVSKDLKDTYIALIEFD 133

Query: 128 PDDYFGTFFDKAYAASALLLFAVLTFVFLLI 146
             D    FF K YA+++LLLFA ++   L +
Sbjct: 134 STDPVDKFFSKGYASASLLLFAFVSLAVLSV 164

The following BLAST results are available for this feature:

BLAST of Carg02352 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7016238.1	2.6e-141	100.00	CASP-like protein 4D1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023551429.1	2.3e-65	95.86	CASP-like protein 4D1 [Cucurbita pepo subsp. pepo]	[more]
XP_022992769.1	1.5e-64	94.48	CASP-like protein 4D1 [Cucurbita maxima]	[more]
XP_022939465.1	3.3e-64	94.48	CASP-like protein 4D1 [Cucurbita moschata]	[more]
XP_023549516.1	2.8e-63	90.97	CASP-like protein 4D1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg02352 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C6T1Z6	2.0e-27	45.70	CASP-like protein 4D1 OS=Glycine max OX=3847 PE=2 SV=1	[more]
B9SXY8	1.6e-24	44.74	CASP-like protein 4D1 OS=Ricinus communis OX=3988 GN=RCOM_1206790 PE=3 SV=1	[more]
B9NBE5	3.9e-23	43.79	CASP-like protein 4D1 OS=Populus trichocarpa OX=3694 GN=POPTR_0010s21230g PE=2 S...	[more]
A1XGB4	1.9e-22	46.15	CASP-like protein PIMP1 OS=Capsicum annuum OX=4072 GN=PIMP1 PE=2 SV=1	[more]
Q56X75	2.0e-19	40.40	CASP-like protein 4D2 OS=Arabidopsis thaliana OX=3702 GN=At2g39518 PE=2 SV=1	[more]

BLAST of Carg02352 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JYE7	7.3e-65	94.48	CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111489007 PE=3 SV=1	[more]
A0A6J1FMS6	1.6e-64	94.48	CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111445361 PE=3 SV=1	[more]
A0A6J1JQU5	2.3e-63	90.32	CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111489006 PE=3 SV=1	[more]
A0A6J1FGY1	4.0e-63	90.32	CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111445381 PE=3 SV=1	[more]
A0A1S3C911	3.3e-57	84.03	CASP-like protein OS=Cucumis melo OX=3656 GN=LOC103498157 PE=3 SV=1	[more]

BLAST of Carg02352 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39518.1	1.4e-20	40.40	Uncharacterised protein family (UPF0497)	[more]
AT2G39530.1	1.2e-19	40.40	Uncharacterised protein family (UPF0497)	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006702	Casparian strip membrane protein domain	PFAM	PF04535	DUF588	coord: 6..138 e-value: 5.7E-20 score: 71.6
None	No IPR available	PANTHER	PTHR11615	NITRATE, FORMATE, IRON DEHYDROGENASE	coord: 137..273
None	No IPR available	PANTHER	PTHR11615	NITRATE, FORMATE, IRON DEHYDROGENASE	coord: 5..137
None	No IPR available	PANTHER	PTHR11615:SF148	CASP-LIKE PROTEIN 4D2	coord: 137..273
None	No IPR available	PANTHER	PTHR11615:SF148	CASP-LIKE PROTEIN 4D2	coord: 5..137

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg02352-RA	Carg02352-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding