Carg02338 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg02338
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	cystinosin homolog
Location	Carg_Chr15: 1879022 .. 1881792 (+)
RNA-Seq Expression	Carg02338
Synteny	Carg02338

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAAAAAGAAGAAGAAAAAAAAGAAAGGACAGGAAAATTTGCGAGGGAAGAACTTCCCGGTGGAATCTCCCATTGATCAGGCGGTAACCATCCGCCTCGTCGTTTTCTCTGCTTCTGAATTTCATCATCGGCTAACGAATCGAATTTCAGGTTGCGACGATAAATTTCTCATTCTTCTTTGTTTCATCTGCCTTTAGCCGAAACAAGGAAACGAAGAAAAATGGCGTCGTGGAATTCGATTCCTCTCGAGATTACCTACGAAGTACTGGGATGGTTCGCCTTCGTTTCCTGGTCCATCAGTTTCTATCCTCAAGTCATCTTAAATTTCCGTAGGAAGAGGTACTTCTCGTTGATTTCCATTTGTTTATTCCATCCGCCTTCTTCTTCTTCTTCCTGATTTTTCATTGTTTTTTGTGTTATGTAGTGTCGTTGGACTGAATTTTGATTTCGTGCTGTTAAACTTGACTAAGCACTCGACATATTTGATATATAACGCCTCGCTTTACTTCAGCTCCGCTGTTCAGAAACAGTACTTTGAGAAGTATGGTTTCGATCAGGTTTTGGACTTCTTCGACTCTGCTTCCTTTTCCTTTTTGTTTTCTCGTTGTGTGTGATCGTGCGGTCATGTTTTGATGTTGGAATTAATTGTTGCATCGGTGGATATATCTCGCCTTTATTGCCGGTTTTATTGTGTTGAACTTAAAATTAACAAACATAATTTGCCTATAAAATCTGGATGTTTAAGTTTGGATTCAATGTCCTGCTACTTGGTGTGAATTTGTGTGTGTTAGTGTTTAACATCTCCACATGTTAGATGGAACTATTTCACATTGTAATTGTTGAGGATTATTGGGATTGGGAGTGTCCCACATTGGTTAATTTAGTGGAAGATCATGGGTTTATAAGTGAGGAATACTAACTCCATTGGTATGAGGCCTTTTGGGGAAGCCCAAAGCAAAGCCATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTGTGGAGAGTCGTGATTCCTAACATGGTATCAGAGCCATGCCCTAAACCTAACCATGCCAATAGAATCCTCAAATATCGAACAAAGAATGGCGAGCCTCGAAGGTGTAGTCAAAAGTGACTAACATGTCGAACAAATGGTGTACTTTGTTCGAGGGCTCCAGAGAAAGGAGTCGAGCCTCAATTAAGGGGAGGCTATTCGAGAGCTCCATAAGCCTCAGGGGAGGCTTATAGTGTGCTTTGTTCGAGGGGAGGTGTTGAGGATTATTGGGATTGGGAGTGTCCCACATTGGTTAATTTAGTGGAAGATCATGGGTTTATAAGTGAGGAATACTAACTCCATTGGTATGAGGCCTTTTGGGGAAGCCCAAAGCAAAGCCATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTGTGGAGAGTCGTGATTCCTAACAGTAATCTCGATCTAGAACTGTGTTTGATACTGTAAATTTTTGTTAATTTCTTCTTACACGTTAGTATATTAACTACAGGTATTCCACTTCATTTGTTAATTTCTCCTTCTACCTTTGTATTTTCTGCTGATCTTGTTGATTTTCTTGGATCTTTCAGATGATACCTGTTGCTGCAAATGATGTTGCTTTCTCTATACACGCTGTTCTGTTGACGGCAATCACGTTGTTCCAAATTGTGATCTATGATGTTAGCCGTTTGGAATTCTTCTCTAAGTTTATATTTGTTCTATCCGCTGGTCTATTTAAAGATTGATTTTGCTAACATATATTAATTACTTTTACTTTTGCAGCGTGGAACTCAGATAGTTTCCAAAATATCGCTTACAATTGTGACTGTCGTATGGATATTTGCTGCAATTTGTTTCTTCATTGGTCTGTCTAGCCAGTCTTGGCTCTGGTTAATCTCCATATTCAAGTAAGGGGGTTCTTAAGAAGTTTCCTGAATGGTGATATTTTATTGGTTGACACTTTAAATCTTTGATCTATTATGTACTGAATCGGTCGAGTCAACACCTATGACAGGAATTCTATAATACCTTATAACTTATAAGGATGTGAAAAGCTACCACAAAATTTTAAAAAATCTGATTGGTGTTTGTCCTAGTACGTAAATTGTTGAATTTCTTTGCTACATAATAACTTCCCAAAAAGAGAGATTCTACATTTAAACACTTTTATTTGCAATATCCTAGCTATAGGTTTCTAGGAAAGCACGGGATACCTTTTCCTGTTATTCAACCAGATATAGTGGTGCAAGAATCAAGATTTGCTTCTTCAAACTCTTTTCGGCCATAAACTCTGGAAAATGTTTCTTTGATAATGACAGATTTTCCCCCTTTAAGAACTTTGATGATGACAGTTGAAGATGTCTTTTTAGGAAATTTCCCAGAGAGAAATTGTAGTGGCTTTGAGAATAGGTGGAAATGTAATGGTTAGATCCTAGAAAAGGTCATGTTTTGGGTTATTAGATGGACTTCTTTGATTGCATCCTTGTGTATTTATCTTCCTCTTGGATTCTATATTTTATTTGGATACGAAATGAAATCACTTCATTAATAGCTAGTAGAAGGACAGGTGCAATATCTGCTCTGCCAGAAACGGGGATGAATCTACACTAACCAAGTTGTATACTTATATAGTACATGAAATGAGGAAAAACCTTTGGATAAAGCACTGCAACTAGATCATACATGATGCGAGATAGTTCTTTGGGGGTTATCTTTTTTAATTCTGTCTTGGATGTATTGAAACTCCCATAAGCAGAAGATCCA

mRNA sequence

AGAAAAAGAAGAAGAAAAAAAAGAAAGGACAGGAAAATTTGCGAGGGAAGAACTTCCCGGTGGAATCTCCCATTGATCAGGCGGTAACCATCCGCCTCGTCGTTTTCTCTGCTTCTGAATTTCATCATCGGCTAACGAATCGAATTTCAGGTTGCGACGATAAATTTCTCATTCTTCTTTGTTTCATCTGCCTTTAGCCGAAACAAGGAAACGAAGAAAAATGGCGTCGTGGAATTCGATTCCTCTCGAGATTACCTACGAAGTACTGGGATGGTTCGCCTTCGTTTCCTGGTCCATCAGTTTCTATCCTCAAGTCATCTTAAATTTCCGTAGGAAGAGTGTCGTTGGACTGAATTTTGATTTCGTGCTGTTAAACTTGACTAAGCACTCGACATATTTGATATATAACGCCTCGCTTTACTTCAGCTCCGCTGTTCAGAAACAGTACTTTGAGAAGTATGGTTTCGATCAGATGATACCTGTTGCTGCAAATGATGTTGCTTTCTCTATACACGCTGTTCTGTTGACGGCAATCACGTTGTTCCAAATTGTGATCTATGATCGTGGAACTCAGATAGTTTCCAAAATATCGCTTACAATTGTGACTGTCGTATGGATATTTGCTGCAATTTGTTTCTTCATTGGTCTGTCTAGCCAGTCTTGGCTCTGGTTAATCTCCATATTCAAGTAAGGGGGTTCTTAAGAAGTTTCCTGAATGGTGATATTTTATTGGTTGACACTTTAAATCTTTGATCTATTATGTACTGAATCGGTCGAGTCAACACCTATGACAGGAATTCTATAATACCTTATAACTTATAAGGATGTGAAAAGCTACCACAAAATTTTAAAAAATCTGATTGGTGTTTGTCCTAGTACGTAAATTGTTGAATTTCTTTGCTACATAATAACTTCCCAAAAAGAGAGATTCTACATTTAAACACTTTTATTTGCAATATCCTAGCTATAGGTTTCTAGGAAAGCACGGGATACCTTTTCCTGTTATTCAACCAGATATAGTGGTGCAAGAATCAAGATTTGCTTCTTCAAACTCTTTTCGGCCATAAACTCTGGAAAATGTTTCTTTGATAATGACAGATTTTCCCCCTTTAAGAACTTTGATGATGACAGTTGAAGATGTCTTTTTAGGAAATTTCCCAGAGAGAAATTGTAGTGGCTTTGAGAATAGGTGGAAATGTAATGGTTAGATCCTAGAAAAGGTCATGTTTTGGGTTATTAGATGGACTTCTTTGATTGCATCCTTGTGTATTTATCTTCCTCTTGGATTCTATATTTTATTTGGATACGAAATGAAATCACTTCATTAATAGCTAGTAGAAGGACAGGTGCAATATCTGCTCTGCCAGAAACGGGGATGAATCTACACTAACCAAGTTGTATACTTATATAGTACATGAAATGAGGAAAAACCTTTGGATAAAGCACTGCAACTAGATCATACATGATGCGAGATAGTTCTTTGGGGGTTATCTTTTTTAATTCTGTCTTGGATGTATTGAAACTCCCATAAGCAGAAGATCCA

Coding sequence (CDS)

ATGGCGTCGTGGAATTCGATTCCTCTCGAGATTACCTACGAAGTACTGGGATGGTTCGCCTTCGTTTCCTGGTCCATCAGTTTCTATCCTCAAGTCATCTTAAATTTCCGTAGGAAGAGTGTCGTTGGACTGAATTTTGATTTCGTGCTGTTAAACTTGACTAAGCACTCGACATATTTGATATATAACGCCTCGCTTTACTTCAGCTCCGCTGTTCAGAAACAGTACTTTGAGAAGTATGGTTTCGATCAGATGATACCTGTTGCTGCAAATGATGTTGCTTTCTCTATACACGCTGTTCTGTTGACGGCAATCACGTTGTTCCAAATTGTGATCTATGATCGTGGAACTCAGATAGTTTCCAAAATATCGCTTACAATTGTGACTGTCGTATGGATATTTGCTGCAATTTGTTTCTTCATTGGTCTGTCTAGCCAGTCTTGGCTCTGGTTAATCTCCATATTCAAGTAA

Protein sequence

MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYLIYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIVSKISLTIVTVVWIFAAICFFIGLSSQSWLWLISIFK

Homology

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BLAST of Carg02338 vs. NCBI nr
Match: KAG7016224.1 (Cystinosin-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 302.0 bits (772), Expect = 3.0e-78
Identity = 156/156 (100.00%), Postives = 156/156 (100.00%), Query Frame = 0


Query: 1   MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL 60
           MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL
Sbjct: 1   MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL 60

Query: 61  IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV 120
           IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV
Sbjct: 61  IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV 120

Query: 121 SKISLTIVTVVWIFAAICFFIGLSSQSWLWLISIFK 157
           SKISLTIVTVVWIFAAICFFIGLSSQSWLWLISIFK
Sbjct: 121 SKISLTIVTVVWIFAAICFFIGLSSQSWLWLISIFK 156

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BLAST of Carg02338 vs. NCBI nr
Match: XP_022939850.1 (cystinosin homolog [Cucurbita moschata] >XP_022939851.1 cystinosin homolog [Cucurbita moschata] >XP_022939852.1 cystinosin homolog [Cucurbita moschata])

HSP 1 Score: 299.7 bits (766), Expect = 1.5e-77
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 0


Query: 1   MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL 60
           MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL
Sbjct: 1   MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL 60

Query: 61  IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV 120
           IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV
Sbjct: 61  IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV 120

Query: 121 SKISLTIVTVVWIFAAICFFIGLSSQSWLWLISIF 156
           SKISLTIVTVVWIFAAICFFIGLSSQSWLWLISIF
Sbjct: 121 SKISLTIVTVVWIFAAICFFIGLSSQSWLWLISIF 155

[Close]

BLAST of Carg02338 vs. NCBI nr
Match: XP_022992807.1 (cystinosin homolog [Cucurbita maxima] >XP_022992808.1 cystinosin homolog [Cucurbita maxima] >XP_022992809.1 cystinosin homolog [Cucurbita maxima])

HSP 1 Score: 297.7 bits (761), Expect = 5.7e-77
Identity = 154/155 (99.35%), Postives = 154/155 (99.35%), Query Frame = 0


Query: 1   MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL 60
           MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL
Sbjct: 1   MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL 60

Query: 61  IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV 120
           IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV
Sbjct: 61  IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV 120

Query: 121 SKISLTIVTVVWIFAAICFFIGLSSQSWLWLISIF 156
           SKISLTIVTVVWIFAAICFFIGLSS SWLWLISIF
Sbjct: 121 SKISLTIVTVVWIFAAICFFIGLSSHSWLWLISIF 155

[Close]

BLAST of Carg02338 vs. NCBI nr
Match: XP_023550396.1 (cystinosin homolog [Cucurbita pepo subsp. pepo] >XP_023550397.1 cystinosin homolog [Cucurbita pepo subsp. pepo] >XP_023550398.1 cystinosin homolog [Cucurbita pepo subsp. pepo] >KAG6578686.1 Cystinosin-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 297.7 bits (761), Expect = 5.7e-77
Identity = 154/155 (99.35%), Postives = 154/155 (99.35%), Query Frame = 0


Query: 1   MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL 60
           MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL
Sbjct: 1   MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL 60

Query: 61  IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV 120
           IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV
Sbjct: 61  IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV 120

Query: 121 SKISLTIVTVVWIFAAICFFIGLSSQSWLWLISIF 156
           SKISLTIVTVVWIFAAICFFIGLSS SWLWLISIF
Sbjct: 121 SKISLTIVTVVWIFAAICFFIGLSSHSWLWLISIF 155

[Close]

BLAST of Carg02338 vs. NCBI nr
Match: XP_038890971.1 (cystinosin homolog [Benincasa hispida])

HSP 1 Score: 295.4 bits (755), Expect = 2.8e-76
Identity = 151/155 (97.42%), Postives = 154/155 (99.35%), Query Frame = 0


Query: 1   MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL 60
           MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL
Sbjct: 1   MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL 60

Query: 61  IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV 120
           IYNASLYFSSAVQKQYFEKYG+DQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV
Sbjct: 61  IYNASLYFSSAVQKQYFEKYGYDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV 120

Query: 121 SKISLTIVTVVWIFAAICFFIGLSSQSWLWLISIF 156
           SK+SLTIVTVVW+FAAICFFIGLSS SWLWLISIF
Sbjct: 121 SKVSLTIVTVVWLFAAICFFIGLSSHSWLWLISIF 155

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BLAST of Carg02338 vs. ExPASy Swiss-Prot
Match: P57758 (Cystinosin homolog OS=Arabidopsis thaliana OX=3702 GN=At5g40670 PE=2 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 2.4e-62
Identity = 117/155 (75.48%), Postives = 137/155 (88.39%), Query Frame = 0


Query: 1   MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL 60
           MASWNSIPLEI+YE++GW AF SWSISFYPQ+ILNFRR+SVVGLNFDFV+LNLTKHS+Y+
Sbjct: 1   MASWNSIPLEISYEIVGWIAFASWSISFYPQLILNFRRRSVVGLNFDFVMLNLTKHSSYM 60

Query: 61  IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV 120
           IYN  LYFS  +QKQYF+ YG  +MIPVAANDVAFSIHAV++TA+TLFQI IY+RG Q V
Sbjct: 61  IYNVCLYFSPVIQKQYFDTYGDKEMIPVAANDVAFSIHAVVMTAVTLFQIFIYERGPQKV 120

Query: 121 SKISLTIVTVVWIFAAICFFIGLSSQSWLWLISIF 156
           S++++ IV VVW FAAICFFI L + SWLWLISIF
Sbjct: 121 SRLAIGIVVVVWGFAAICFFIALPTHSWLWLISIF 155

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BLAST of Carg02338 vs. ExPASy Swiss-Prot
Match: Q54WT7 (Cystinosin homolog OS=Dictyostelium discoideum OX=44689 GN=ctns PE=3 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.2e-32
Identity = 69/148 (46.62%), Postives = 104/148 (70.27%), Query Frame = 0


Query: 9   LEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYLIYNASLYF 68
           L I   ++GW  F  WS+SFYPQVILNFR+K+V+GL+FDF+L N+T ++ Y ++N+ LYF
Sbjct: 4   LSIISIIIGWIYFACWSLSFYPQVILNFRKKNVIGLSFDFLLFNITGYACYSVFNSVLYF 63

Query: 69  SSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIVSKISLTIV 128
              V+ +Y++KYG    IPV  +D+AF+IH  +LTAIT+ Q  IYDRG Q  SK+ + I 
Sbjct: 64  DKLVKNEYYDKYG--PPIPVQQSDIAFAIHGFVLTAITIIQCFIYDRGNQKNSKLGIGIA 123

Query: 129 TVVWIFAAICFFIGLSSQ-SWLWLISIF 156
           T++W+   +   +G S+  +WLW+I+ +
Sbjct: 124 TLIWVSLIVMTILGFSNVFTWLWVINYY 149

[Close]

BLAST of Carg02338 vs. ExPASy Swiss-Prot
Match: A7MB63 (Cystinosin OS=Bos taurus OX=9913 GN=CTNS PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 4.7e-26
Identity = 62/148 (41.89%), Postives = 101/148 (68.24%), Query Frame = 0


Query: 9   LEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYLIYNASLYF 68
           + I  +V+GW  FV+WS+SFYPQVI N+RRKSVVGL+FDFV+LNL     Y ++N  L++
Sbjct: 123 VSIINQVIGWIYFVAWSVSFYPQVITNWRRKSVVGLSFDFVVLNLMGFVAYSVFNIGLFW 182

Query: 69  SSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIVSKISLTIV 128
             ++++Q+  KY  + + PV +NDV FS+HAV LT + + Q ++Y+RG+Q VS ++++ +
Sbjct: 183 VPSIKEQFLLKYP-NGVNPVDSNDVFFSLHAVALTLVVIVQCLLYERGSQRVSWLAISFL 242

Query: 129 TVVWIFAAICFFI-GLSSQSWLWLISIF 156
            + W+F  I   +  + + +WL  +  F
Sbjct: 243 VLSWLFTLIALIMAAVGATTWLQFLFCF 269

[Close]

BLAST of Carg02338 vs. ExPASy Swiss-Prot
Match: O60931 (Cystinosin OS=Homo sapiens OX=9606 GN=CTNS PE=1 SV=2)

HSP 1 Score: 116.7 bits (291), Expect = 2.4e-25
Identity = 65/151 (43.05%), Postives = 98/151 (64.90%), Query Frame = 0


Query: 6   SIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYLIYNAS 65
           S  + I  +V+GW  FV+WSISFYPQVI+N+RRKSV+GL+FDFV LNLT    Y ++N  
Sbjct: 120 SSAISIINQVIGWIYFVAWSISFYPQVIMNWRRKSVIGLSFDFVALNLTGFVAYSVFNIG 179

Query: 66  LYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIVSKISL 125
           L +   +++Q+  KY  + + PV +NDV FS+HAV+LT I + Q  +Y+RG Q VS  ++
Sbjct: 180 LLWVPYIKEQFLLKYP-NGVNPVNSNDVFFSLHAVVLTLIIIVQCCLYERGGQRVSWPAI 239

Query: 126 TIVTVVWIFAAICFFI-GLSSQSWLWLISIF 156
             + + W+FA +   +  +   +WL  +  F
Sbjct: 240 GFLVLAWLFAFVTMIVAAVGVTTWLQFLFCF 269

[Close]

BLAST of Carg02338 vs. ExPASy Swiss-Prot
Match: P57757 (Cystinosin OS=Mus musculus OX=10090 GN=Ctns PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.3e-23
Identity = 60/148 (40.54%), Postives = 93/148 (62.84%), Query Frame = 0


Query: 9   LEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYLIYNASLYF 68
           + I  +V+GW  F++WS+SFYPQVI N+RRKSV+GL+FDF+ LNLT    Y ++N  L +
Sbjct: 123 VSIINQVIGWIYFMAWSVSFYPQVIQNWRRKSVIGLSFDFLALNLTGFVAYSVFNIGLLW 182

Query: 69  SSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIVSKISLTIV 128
              +Q+++  KY  + + PV +ND  FS+HAV LT I + Q  +Y+RG Q VS  S+  +
Sbjct: 183 VPYIQEEFLLKYP-NGVNPVDSNDAFFSLHAVALTLIVILQCCLYERGNQRVSWPSIGFL 242

Query: 129 TVVWIFAAICFFI-GLSSQSWLWLISIF 156
            + W+F  +   +  +   +WL  +  F
Sbjct: 243 VLAWLFVLVTMIVAAVGITTWLQFLFCF 269

[Close]

BLAST of Carg02338 vs. ExPASy TrEMBL
Match: A0A6J1FNX3 (cystinosin homolog OS=Cucurbita moschata OX=3662 GN=LOC111445597 PE=4 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 7.2e-78
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 0


Query: 1   MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL 60
           MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL
Sbjct: 1   MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL 60

Query: 61  IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV 120
           IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV
Sbjct: 61  IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV 120

Query: 121 SKISLTIVTVVWIFAAICFFIGLSSQSWLWLISIF 156
           SKISLTIVTVVWIFAAICFFIGLSSQSWLWLISIF
Sbjct: 121 SKISLTIVTVVWIFAAICFFIGLSSQSWLWLISIF 155

[Close]

BLAST of Carg02338 vs. ExPASy TrEMBL
Match: A0A6J1K0A0 (cystinosin homolog OS=Cucurbita maxima OX=3661 GN=LOC111489029 PE=4 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 2.8e-77
Identity = 154/155 (99.35%), Postives = 154/155 (99.35%), Query Frame = 0


Query: 1   MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL 60
           MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL
Sbjct: 1   MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL 60

Query: 61  IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV 120
           IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV
Sbjct: 61  IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV 120

Query: 121 SKISLTIVTVVWIFAAICFFIGLSSQSWLWLISIF 156
           SKISLTIVTVVWIFAAICFFIGLSS SWLWLISIF
Sbjct: 121 SKISLTIVTVVWIFAAICFFIGLSSHSWLWLISIF 155

[Close]

BLAST of Carg02338 vs. ExPASy TrEMBL
Match: A0A0A0KNW7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G517770 PE=4 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 7.5e-75
Identity = 148/155 (95.48%), Postives = 153/155 (98.71%), Query Frame = 0


Query: 1   MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL 60
           MASWNSIPLEITY+VLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL
Sbjct: 1   MASWNSIPLEITYQVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL 60

Query: 61  IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV 120
           IYNASLYFSSAVQKQYF+KYG+DQMIPVAA+DVAFSIHAVLLTAITLFQ+VIYDRGTQIV
Sbjct: 61  IYNASLYFSSAVQKQYFDKYGYDQMIPVAASDVAFSIHAVLLTAITLFQVVIYDRGTQIV 120

Query: 121 SKISLTIVTVVWIFAAICFFIGLSSQSWLWLISIF 156
           SKISLTIV VVWIFAAICFFIGLSS SWLWLISIF
Sbjct: 121 SKISLTIVIVVWIFAAICFFIGLSSHSWLWLISIF 155

[Close]

BLAST of Carg02338 vs. ExPASy TrEMBL
Match: A0A5D3DGP9 (Cystinosin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold311G00130 PE=4 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 9.8e-75
Identity = 149/155 (96.13%), Postives = 151/155 (97.42%), Query Frame = 0


Query: 1   MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL 60
           MASWNSIPLEI Y+VLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL
Sbjct: 1   MASWNSIPLEIIYQVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL 60

Query: 61  IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV 120
           IYNASLYFSSAVQKQYFEKYG+DQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV
Sbjct: 61  IYNASLYFSSAVQKQYFEKYGYDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV 120

Query: 121 SKISLTIVTVVWIFAAICFFIGLSSQSWLWLISIF 156
           SKISLTIV  VWIFAAICFFIGLSS SWLWLISIF
Sbjct: 121 SKISLTIVIAVWIFAAICFFIGLSSHSWLWLISIF 155

[Close]

BLAST of Carg02338 vs. ExPASy TrEMBL
Match: A0A1S3C8Y4 (cystinosin homolog OS=Cucumis melo OX=3656 GN=LOC103498135 PE=4 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 9.8e-75
Identity = 149/155 (96.13%), Postives = 151/155 (97.42%), Query Frame = 0


Query: 1   MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL 60
           MASWNSIPLEI Y+VLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL
Sbjct: 1   MASWNSIPLEIIYQVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL 60

Query: 61  IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV 120
           IYNASLYFSSAVQKQYFEKYG+DQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV
Sbjct: 61  IYNASLYFSSAVQKQYFEKYGYDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV 120

Query: 121 SKISLTIVTVVWIFAAICFFIGLSSQSWLWLISIF 156
           SKISLTIV  VWIFAAICFFIGLSS SWLWLISIF
Sbjct: 121 SKISLTIVIAVWIFAAICFFIGLSSHSWLWLISIF 155

[Close]

BLAST of Carg02338 vs. TAIR 10
Match: AT5G40670.1 (PQ-loop repeat family protein / transmembrane family protein )

HSP 1 Score: 239.6 bits (610), Expect = 1.7e-63
Identity = 117/155 (75.48%), Postives = 137/155 (88.39%), Query Frame = 0


Query: 1   MASWNSIPLEITYEVLGWFAFVSWSISFYPQVILNFRRKSVVGLNFDFVLLNLTKHSTYL 60
           MASWNSIPLEI+YE++GW AF SWSISFYPQ+ILNFRR+SVVGLNFDFV+LNLTKHS+Y+
Sbjct: 1   MASWNSIPLEISYEIVGWIAFASWSISFYPQLILNFRRRSVVGLNFDFVMLNLTKHSSYM 60

Query: 61  IYNASLYFSSAVQKQYFEKYGFDQMIPVAANDVAFSIHAVLLTAITLFQIVIYDRGTQIV 120
           IYN  LYFS  +QKQYF+ YG  +MIPVAANDVAFSIHAV++TA+TLFQI IY+RG Q V
Sbjct: 61  IYNVCLYFSPVIQKQYFDTYGDKEMIPVAANDVAFSIHAVVMTAVTLFQIFIYERGPQKV 120

Query: 121 SKISLTIVTVVWIFAAICFFIGLSSQSWLWLISIF 156
           S++++ IV VVW FAAICFFI L + SWLWLISIF
Sbjct: 121 SRLAIGIVVVVWGFAAICFFIALPTHSWLWLISIF 155

The following BLAST results are available for this feature:

BLAST of Carg02338 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7016224.1	3.0e-78	100.00	Cystinosin-like protein [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022939850.1	1.5e-77	100.00	cystinosin homolog [Cucurbita moschata] >XP_022939851.1 cystinosin homolog [Cucu...	[more]
XP_022992807.1	5.7e-77	99.35	cystinosin homolog [Cucurbita maxima] >XP_022992808.1 cystinosin homolog [Cucurb...	[more]
XP_023550396.1	5.7e-77	99.35	cystinosin homolog [Cucurbita pepo subsp. pepo] >XP_023550397.1 cystinosin homol...	[more]
XP_038890971.1	2.8e-76	97.42	cystinosin homolog [Benincasa hispida]	[more]

BLAST of Carg02338 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P57758	2.4e-62	75.48	Cystinosin homolog OS=Arabidopsis thaliana OX=3702 GN=At5g40670 PE=2 SV=1	[more]
Q54WT7	1.2e-32	46.62	Cystinosin homolog OS=Dictyostelium discoideum OX=44689 GN=ctns PE=3 SV=1	[more]
A7MB63	4.7e-26	41.89	Cystinosin OS=Bos taurus OX=9913 GN=CTNS PE=2 SV=1	[more]
O60931	2.4e-25	43.05	Cystinosin OS=Homo sapiens OX=9606 GN=CTNS PE=1 SV=2	[more]
P57757	1.3e-23	40.54	Cystinosin OS=Mus musculus OX=10090 GN=Ctns PE=1 SV=1	[more]

BLAST of Carg02338 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FNX3	7.2e-78	100.00	cystinosin homolog OS=Cucurbita moschata OX=3662 GN=LOC111445597 PE=4 SV=1	[more]
A0A6J1K0A0	2.8e-77	99.35	cystinosin homolog OS=Cucurbita maxima OX=3661 GN=LOC111489029 PE=4 SV=1	[more]
A0A0A0KNW7	7.5e-75	95.48	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G517770 PE=4 SV=1	[more]
A0A5D3DGP9	9.8e-75	96.13	Cystinosin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3C8Y4	9.8e-75	96.13	cystinosin homolog OS=Cucumis melo OX=3656 GN=LOC103498135 PE=4 SV=1	[more]

BLAST of Carg02338 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G40670.1	1.7e-63	75.48	PQ-loop repeat family protein / transmembrane family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006603	PQ-loop repeat	SMART	SM00679	ctns	coord: 26..57 e-value: 2.5E-7 score: 40.3
IPR006603	PQ-loop repeat	PFAM	PF04193	PQ-loop	coord: 14..69 e-value: 1.7E-19 score: 69.3
None	No IPR available	GENE3D	1.20.1280.290		coord: 12..122 e-value: 2.7E-11 score: 45.4
None	No IPR available	PANTHER	PTHR13131:SF11	LYSOSOMAL CYSTINE TRANSPORTER FAMILY PROTEIN	coord: 1..155
IPR005282	Lysosomal cystine transporter	PANTHER	PTHR13131	CYSTINOSIN	coord: 1..155

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg02338-RA	Carg02338-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015811	L-cystine transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005774	vacuolar membrane
molecular_function	GO:0015184	L-cystine transmembrane transporter activity